AlfalfaGEDB | Msa1241950

M. sativa cultivar zhongmu-4 / Msa1241950

KEGG: K19613|K22038
GO: -
PFAM: PF01582.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1241950	XP_013445175.1	83.145	1507	185	7	1	1449	1	1496	0.0	2448

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1241950	sp\|A0A290U7C4\|ROQ1_NICBE	34.736	927	520	22	9	889	12	899	1.68e-136	457

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1241950	A0A072TPQ2	83.145	1507	185	7	1	1449	1	1496	0.0	2448

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1187720	Msa1241950	0.813733	2.204115e-51	-8.615850e-47
Msa1187730	Msa1241950	0.807642	4.574665e-50	-8.615850e-47
Msa1187740	Msa1241950	0.820474	6.730461e-53	-8.615850e-47
Msa1241950	Msa1322370	0.805359	1.386716e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1241950	MtrunA17_Chr8g0354721	83.145	1507	185	7	1	1449	1	1496	2448
Msa1241950	MtrunA17_Chr8g0354711	55.826	1519	540	21	1	1443	1	1464	1502
Msa1241950	MtrunA17_Chr8g0354701	54.970	1519	553	24	1	1443	1	1464	1492
Msa1241950	MtrunA17_Chr8g0354681	67.955	1061	291	10	1	1021	1	1052	1413
Msa1241950	MtrunA17_Chr8g0353371	47.294	1127	508	21	1	1061	1	1107	901
Msa1241950	MtrunA17_Chr6g0482511	47.387	1110	463	18	6	1061	30	1072	890
Msa1241950	MtrunA17_Chr8g0371711	46.818	1100	462	17	7	1061	18	1039	878
Msa1241950	MtrunA17_Chr6g0481011	45.009	1102	484	18	7	1061	21	1047	847
Msa1241950	MtrunA17_Chr6g0480911	46.175	1111	468	23	7	1061	16	1052	846
Msa1241950	MtrunA17_Chr4g0006771	45.714	1015	426	11	7	979	17	948	817

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1241950	AT5G36930.3	33.976	986	555	21	9	942	14	955	1.31e-133	442
Msa1241950	AT5G36930.2	33.976	986	555	21	9	942	17	958	5.16e-133	442
Msa1241950	AT5G36930.4	33.976	986	555	21	9	942	14	955	5.53e-133	441
Msa1241950	AT5G36930.1	33.976	986	555	21	9	942	14	955	5.53e-133	441
Msa1241950	AT5G41750.1	30.478	1109	573	33	9	1052	13	988	2.43e-123	412
Msa1241950	AT5G41750.2	30.478	1109	573	33	9	1052	13	988	2.43e-123	412
Msa1241950	AT5G17680.1	33.333	933	527	22	10	894	19	904	3.36e-123	417
Msa1241950	AT5G17680.1	24.700	417	232	10	597	959	736	1124	1.12e-18	93.6
Msa1241950	AT5G17680.2	33.333	933	528	22	10	894	19	905	7.08e-123	416
Msa1241950	AT5G17680.2	24.700	417	232	10	597	959	737	1125	1.18e-18	93.2

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Find 310 sgRNAs with CRISPR-Local

Find 447 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTATTATTAGAAA+TGG	0.388809	8_2:+25851484	Msa1241950:CDS
AAAACTCTCTATTAGTGGCC+TGG	0.497251	8_2:+25853946	Msa1241950:CDS
AAACTCACATTTAGCATTGG+TGG	0.494777	8_2:-25853299	None:intergenic
AAAGAACAACTAGATGCCAA+AGG	0.647853	8_2:+25854484	Msa1241950:CDS
AAAGATGAAGCGCACATGAA+AGG	0.551017	8_2:+25851403	Msa1241950:CDS
AAAGCCATGTGAGCCAATTC+AGG	0.395479	8_2:+25851633	Msa1241950:CDS
AAAGTGTACGATTAACCTTG+AGG	0.678077	8_2:-25850076	None:intergenic
AAATAACAGAAATTCAAATA+AGG	0.290026	8_2:+25852110	Msa1241950:CDS
AAATCTCTGATAAAGATTGA+AGG	0.545917	8_2:+25850966	Msa1241950:CDS
AAATCTTGAGGAGAGATTCA+AGG	0.523711	8_2:+25849295	Msa1241950:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAGAAGACATCATAGTAAA+AGG	-	chr8_2:25848946-25848965	None:intergenic	30.0%
GATGATAAGGAAATTCGAAA+AGG	+	chr8_2:25849050-25849069	Msa1241950:CDS	30.0%
TTAATTGCCTTAACAAGTGA+TGG	-	chr8_2:25849089-25849108	None:intergenic	30.0%
AAAAACAATGATGGCAATCT+TGG	-	chr8_2:25849117-25849136	None:intergenic	30.0%
GGAAAGTTCATCTAAACAAA+AGG	-	chr8_2:25849162-25849181	None:intergenic	30.0%
TTTCTAACAAGATAAGCCAA+AGG	-	chr8_2:25849427-25849446	None:intergenic	30.0%
GAAATTGTAAGGAGCAATTT+AGG	+	chr8_2:25849546-25849565	Msa1241950:intron	30.0%
AACAACAAAAACCTTCAAAG+TGG	-	chr8_2:25850265-25850284	None:intergenic	30.0%
ATCTCCAACAAATGTATACT+TGG	+	chr8_2:25850330-25850349	Msa1241950:CDS	30.0%
TGAGGTTAAAAGAACATATG+AGG	+	chr8_2:25850554-25850573	Msa1241950:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	25848927	25854765	25848927	ID=Msa1241950;Name=Msa1241950
chr8_2	mRNA	25848927	25854765	25848927	ID=Msa1241950-mRNA-1;Parent=Msa1241950;Name=Msa1241950-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0\|0.16\|0\|0.71\|1\|1\|7\|0\|1449
chr8_2	exon	25848927	25849396	25848927	ID=Msa1241950-mRNA-1:exon:9122;Parent=Msa1241950-mRNA-1
chr8_2	exon	25850028	25850241	25850028	ID=Msa1241950-mRNA-1:exon:9123;Parent=Msa1241950-mRNA-1
chr8_2	exon	25850327	25851124	25850327	ID=Msa1241950-mRNA-1:exon:9124;Parent=Msa1241950-mRNA-1
chr8_2	exon	25851364	25851672	25851364	ID=Msa1241950-mRNA-1:exon:9125;Parent=Msa1241950-mRNA-1
chr8_2	exon	25851821	25852762	25851821	ID=Msa1241950-mRNA-1:exon:9126;Parent=Msa1241950-mRNA-1
chr8_2	exon	25852849	25853317	25852849	ID=Msa1241950-mRNA-1:exon:9127;Parent=Msa1241950-mRNA-1
chr8_2	exon	25853618	25854765	25853618	ID=Msa1241950-mRNA-1:exon:9128;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25848927	25849396	25848927	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25850028	25850241	25850028	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25850327	25851124	25850327	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25851364	25851672	25851364	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25851821	25852762	25851821	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25852849	25853317	25852849	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1
chr8_2	CDS	25853618	25854765	25853618	ID=Msa1241950-mRNA-1:cds;Parent=Msa1241950-mRNA-1

Gene Sequence

>Msa1241950

ATGGCTGGTCGAGGTTCCTTTTACTATGATGTCTTCTTCAGCTTCAGAGGTGAAGACACAAGACTTGGTTTTACAGGAAACCTCTACAGTGCTTTGAGTCAACGAGGAATCCACAGCTTCTTTGATGATAAGGAAATTCGAAAAGGAGATGAAATAACTCCATCACTTGTTAAGGCAATTAAAGAATCCAAGATTGCCATCATTGTTTTTTCTCAGAACTATGCTTTTTCTTCCTTTTGTTTAGATGAACTTTCCACCATTCTAGAGTGCTATGAGGAGAACAAGGGTCAGCTTGTTTTACCTGTTTTCTTTAACGTTGATCCTTCTGATGTACGACACTGCAGAGGAAAATATGGTCAAGCATTGGAAAATCTTGAGGAGAGATTCAAGGGTGACAAGGAGAAACTCAACAAATGGAGGGTTGCTCTACACAAGGCAGCTAGCATAGCTGGCTTCACTTTCAAGAATGGGAATGGATATGAATGTGAATTCATTTGGCGTATCTTCAAGGAAGTCTCCCTCAAGGTTAATCGTACACTTTTACATGTTGCGAATTATCCAGTTGGATTGGAGTCTCGAGTGGATGAAGTAATCTCACGTCTGAATGTTGGCTCTGATGACGTGGTTCACATGGTAGGTATACATGGAATTGGGGGAATTGGTAAAAGTACTATTGCTCGAGCTGTCATCTCCAACAAATGTATACTTGGAGACGAGGATTTTAAGTTGAGAAATGTCAGCAATGGGATGCCAATGATAGAGCAAAGGCTCTGCAGTAAAAAGGTTCTTTTGGTTCTTGATGATGTTGACAAAATAGAGCAGTTGCAGGCTATTGCTGGAGCACCTAATTGGTTTGGTTTGGGTAGCAGAGTAATCATTACAACTCGGGGCTTAGGCTTACTAAAACATCATGAGGTTAAAAGAACATATGAGGTGGAGGAGTTGAATGACAAAGAAGCTCGTGAATTGCTTTGTTGGAATGCTTTCAAAACTGACAAAGTTGATTCAAGATACACCGCCATTTTAAACCGTGCAGTGGGTTATGCTTCTAGGCTTCCGTTGGCTTTGGAAGTAATAGGTTCCAACTTGTTCGGAAAAGGGGACGACGAATGCACACGTACATTAGATCGGTACGACCGATTTCCAGATAAAAAGATCAATGACATACTTAAAGTCAGCTATGATTCCTTGGAGGAAGATGAGGGGAATGTTTTTCTTGACATTGCTTGTTTCTTCAATGGACATAAGTTGGCAGATGTTGAAGATATACTTTCGGCTCATTATGGTGTCTCAGTGAAATATAGCATTGAAGAACTGATTGGAAAATCTCTGATAAAGATTGAAGGTGGTCTTATGACATTACATTACCTGATACAAGACATGGGCAGAGAAATTGTCCGACTTGAATCACCACATGAGCCTGGCAAACGTAGTAGGTTATGGTTTTGTGAAGATGTAGTCCAAGTTTTAAATGAAAATAAGGGAAGTAGCAAAATTCATATCTTAATTCTGGATTTTCCTAAAGATGAAGCGCACATGAAAGGACCGAAAGGTGAAGAAGTAAATTGGGATGGAGAGGCCTTCAAGGAGATGAAGAACCTCAAAACACTTATTATTAGAAATGGTAGTTTCTCCAAAGGCCCCACACATCTTCCAAATAGGTTAAGAGTTTTGAAATGGCAAGGATATTCTTTACCATGTTATTTCCATCCAAAGAAACTTTCCATACTCGAGTTACCTGATAGCTGCTTAAAGCCATGTGAGCCAATTCAGGCAAGTGACTGTGCGACTGCATTTTCACTTTTGACAATTTTGGATTTTAGCAACAGTGAATCTGTAACTGAGATACCTGATGTATCTGGTGTCCGAAATTTAGAAAAATTATCATTTAGACGTTGTGAGAATTTAGCTAAAGTTCATGACTCAGTTGGACGTCTGGGAAAGCTTATGATATTGGATGCTTCTGGTTGCAAGAATCTGAAGACTTTTCCACCCATCATGTTGACCTCTCTTGAACAACTCAATCTTTCACATTGTTCAATTCTTGAGAGTTTCCCAGAAATATTGGGGAAGATGGAAAAAATAACAGAAATTCAAATAAGGGGGAGTCCTATAAAGGAATATCCATTTTCAATTCAAAACCTTGCTCGGCTTCAAAAATTGGAATTGCAAATGTGTGGAATGGTTCAGTTACCTAGTACCATTTTTATGTTGCCTGAACTAAGTTTGATGCATGTTTCAAAATGTCAAGGGTTGGCCTTATCTGAACAAGAAAAATGTGATAAAACGGTGCTCAAGTCTTCAAACGTGAATCATCTTGTTCTCTCAAACTGCAATATATCCAATGAATTTCTTCCAATAGGTCTTAGCTTTTTCTTCAATGTGAAAGATTTAAACCTATCAGGTAATAGTTTCATAACTCTTCATGCATGGATCAAAGAGTGTCATCTTTTGAGGAATCTTAAGATGGATGATTGCAGTCGTCTTCGAGAAATTATAGGGATTCCGAGGAAATTAGAAAGAATTTCTGTGAAAGGATGCATATCCTTGAATTGCTTAGACCTCGGAGTTCTTCCTGAATGCACTGAACAAAGTTGCTTTCTGAAGGAACTGATTTTGGATGACTGCGTGTGTCTTCGAGAAATTACATGGCTTCCATCAAACTTGGACAGTTTTTCTGCAAAAGGATGCACATCCTTGACTTCCCAGATTATTAGCATGTTATGTAATCAGGAATGGGTAGAAGCGGGAAACAAAAAGTTCTTTTTTCCGGGAAAAAAAATTCCAAAGTGGTTCGAGCATCGTACAAGGGGAGGGTCAATGTCTTTCTGGTTTCGTAACAAATTCCCTGCTATTTCTCTCTGTCTTGTTATCGGACACGGGGATGAGAAACATATCAAGGTCAAGTTTAGCCCTCGAGTATGCATCAATGGCAATAAACAGTTGCTTGGCTGTCAGAAAATCTACGAGTTCATGATAGCAACAGATCATGTGCTTTTGCTAAAATTTGAAGATAACGAGGACGTAGTGTTTTCAGATAATGAGTGGAATCACGTGGAGGTGTCATATGCAGATCATATTACCAACAGTGAGGACTCTATCAGAGAGGTGGCTAGCTATTGCGGAATCCATGTATTATATGAACAAACATCTGGTTCGAGGAATGTCCAATTTCATCCTCCACCACCAATGCTAAATGTGAAGAAACCCCCGAAAGATCTGAATACGGTGTTATCTTTGACGCAACCTCAACTTAATGATGGAGATAAGGTCCTAGCCCCTCAACCAATAATTCCAATTAAGAGTCCTTTGGAGGATGTCATAGGAGAAACTTCAAAAAATGGTAGAGAGGATGAAGAAGCTTATGCTATTACAATCCAAAATGACCATCCTTGCATTGAAAGACGTCATGAGCATGATGCTGTCCAACCGGAATCAGTATCCTCTTGTGAAATTCAATCCGAAGAGTCTACGTCTACTTTCAACACTAAAGATTCTGACTCAGATGACCCCTTCGACCGAGTTGGTAGAAAACTCTCTATTAGTGGCCTGGAAGCCACATCTCCTGGTGCGAAATCTAAAGAGTCTTCTTCCAACACTAAAGGGTCACACTCAGATTATGTCATCGACTTAGTTGATAGAAAACCCTCCGTTAATGGCCATATAACCATATCTTCTAGGGCTGCTTCTCTTGGATCAATCAAAGAGGCTATCAATGCCTTGGAGCTGTTAATGGTTAAGGACTTATCTGAAGTCTCTTCTGATCCTGCTACACAGTTTGGACTTCATCAGCTATTGGATGTATTGAATAGAAGGACAGCTGAGGTGCAAGAGGCTATTGGGGAGTTCAAAAGAAAGGCAGTCGCATCATGCCAAGAATTCCAATCCACCGTTGAGTCTGTGAACAAACTGAAGAGTTATGAGAAGCATCTAGTGAGAATTCAGCAGGAGACTGTCACAAGCAAGGGTCGACGGAATGATCTCAAAAACTCAATGAAAGAGATTTCTTTGGTCATCAAGGCTGAAAATAAGAGGAAGAAGGAGCTAGAGGCAGAAATTGATAGTTTGAAAGAACAACTAGATGCCAAAGGAAGAGACCTTGAACAACTTGTGTTGAATCTCAAGAATAAGGAAACAACACTCTCAACATATTCAACCAATTGTGCTTCCTTGAATGATAAAGCTCAAAAGCTATTAGAAGAAGCTGATTTTCTACTTGCTGCTAAAGATGAGGGTGAAGCTGCTGAAGTAAAGCAGAATAGGCTCAAGTCAACCTGGTCCTCTGACATAAATCTGCAGATTAGTAAAATCAAGCATAACATTTTTGGTTCAGTTCACAATGAATGTTGA

Protein sequence

>Msa1241950

MAGRGSFYYDVFFSFRGEDTRLGFTGNLYSALSQRGIHSFFDDKEIRKGDEITPSLVKAIKESKIAIIVFSQNYAFSSFCLDELSTILECYEENKGQLVLPVFFNVDPSDVRHCRGKYGQALENLEERFKGDKEKLNKWRVALHKAASIAGFTFKNGNGYECEFIWRIFKEVSLKVNRTLLHVANYPVGLESRVDEVISRLNVGSDDVVHMVGIHGIGGIGKSTIARAVISNKCILGDEDFKLRNVSNGMPMIEQRLCSKKVLLVLDDVDKIEQLQAIAGAPNWFGLGSRVIITTRGLGLLKHHEVKRTYEVEELNDKEARELLCWNAFKTDKVDSRYTAILNRAVGYASRLPLALEVIGSNLFGKGDDECTRTLDRYDRFPDKKINDILKVSYDSLEEDEGNVFLDIACFFNGHKLADVEDILSAHYGVSVKYSIEELIGKSLIKIEGGLMTLHYLIQDMGREIVRLESPHEPGKRSRLWFCEDVVQVLNENKGSSKIHILILDFPKDEAHMKGPKGEEVNWDGEAFKEMKNLKTLIIRNGSFSKGPTHLPNRLRVLKWQGYSLPCYFHPKKLSILELPDSCLKPCEPIQASDCATAFSLLTILDFSNSESVTEIPDVSGVRNLEKLSFRRCENLAKVHDSVGRLGKLMILDASGCKNLKTFPPIMLTSLEQLNLSHCSILESFPEILGKMEKITEIQIRGSPIKEYPFSIQNLARLQKLELQMCGMVQLPSTIFMLPELSLMHVSKCQGLALSEQEKCDKTVLKSSNVNHLVLSNCNISNEFLPIGLSFFFNVKDLNLSGNSFITLHAWIKECHLLRNLKMDDCSRLREIIGIPRKLERISVKGCISLNCLDLGVLPECTEQSCFLKELILDDCVCLREITWLPSNLDSFSAKGCTSLTSQIISMLCNQEWVEAGNKKFFFPGKKIPKWFEHRTRGGSMSFWFRNKFPAISLCLVIGHGDEKHIKVKFSPRVCINGNKQLLGCQKIYEFMIATDHVLLLKFEDNEDVVFSDNEWNHVEVSYADHITNSEDSIREVASYCGIHVLYEQTSGSRNVQFHPPPPMLNVKKPPKDLNTVLSLTQPQLNDGDKVLAPQPIIPIKSPLEDVIGETSKNGREDEEAYAITIQNDHPCIERRHEHDAVQPESVSSCEIQSEESTSTFNTKDSDSDDPFDRVGRKLSISGLEATSPGAKSKESSSNTKGSHSDYVIDLVDRKPSVNGHITISSRAASLGSIKEAINALELLMVKDLSEVSSDPATQFGLHQLLDVLNRRTAEVQEAIGEFKRKAVASCQEFQSTVESVNKLKSYEKHLVRIQQETVTSKGRRNDLKNSMKEISLVIKAENKRKKELEAEIDSLKEQLDAKGRDLEQLVLNLKNKETTLSTYSTNCASLNDKAQKLLEEADFLLAAKDEGEAAEVKQNRLKSTWSSDINLQISKIKHNIFGSVHNEC*