AlfalfaGEDB | Msa1191650

M. sativa cultivar zhongmu-4 / Msa1191650

KEGG: K22374
GO: GO:0003674 GO:0003824 GO:0006518 GO:0006807 GO:0006950 GO:0007154 GO:0008150 GO:0008152 GO:0009058 GO:0009237 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0016491 GO:0016614 GO:0016616 GO:0019184 GO:0019290 GO:0019748 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0033707 GO:0034224 GO:0034641 GO:0042594 GO:0043043 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044550 GO:0050896 GO:0051186 GO:0051188 GO:0051716 GO:0055114 GO:0071496 GO:0071704 GO:0120127 GO:1901564 GO:1901566 GO:1901576 GO:1990641
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1191650	sp\|E7C196\|MER_ERYCB	57.353	68	28	1	1	67	39	106	4.30e-20	84.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1191650	A0A396I9I4	87.097	62	8	0	6	67	46	107	9.37e-31	113

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0506110	Msa1191650	0.813893	2.032629e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1191650	MtrunA17_Chr4g0036291	87.097	62	8	0	6	67	46	107	1.80e-34	113
Msa1191650	MtrunA17_Chr4g0036251	83.871	62	10	0	6	67	40	101	7.84e-34	111
Msa1191650	MtrunA17_Chr4g0036311	83.871	62	10	0	6	67	46	107	8.83e-34	111
Msa1191650	MtrunA17_Chr6g0480011	62.295	61	23	0	7	67	47	107	1.06e-20	82.8
Msa1191650	MtrunA17_Chr6g0479981	56.061	66	29	0	7	72	47	112	7.06e-19	77.8
Msa1191650	MtrunA17_Chr1g0173621	60.000	60	24	0	7	66	50	109	1.65e-17	74.3
Msa1191650	MtrunA17_Chr6g0480021	78.049	41	9	0	7	47	47	87	2.68e-16	70.9
Msa1191650	MtrunA17_Chr3g0121001	48.387	62	32	0	5	66	48	109	1.36e-14	66.2
Msa1191650	MtrunA17_Chr5g0447951	51.613	62	29	1	5	66	47	107	8.86e-13	61.2
Msa1191650	MtrunA17_Chr5g0447961	51.613	62	29	1	5	66	47	107	8.86e-13	61.2
Msa1191650	MtrunA17_Chr5g0447941	51.613	62	29	1	5	66	47	107	1.01e-12	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1191650	AT1G59960.1	53.333	60	28	0	7	66	51	110	1.36e-16	72.0
Msa1191650	AT1G59950.1	53.333	60	28	0	7	66	45	104	1.72e-16	72.0

Find 8 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGTAATCTTTGCTTGAGAA+TGG	0.411031	8_1:-36355533	None:intergenic
TGCATGCTTGTAAGAACTAC+AGG	0.412849	8_1:+36355347	Msa1191650:CDS
AACATCAGTACACGATAACT+TGG	0.492306	8_1:-36355479	None:intergenic
GTGTCAAAAGATTTAGAGCT+AGG	0.520940	8_1:+36355421	Msa1191650:CDS
TCAAGGTCTAGCTCATAACT+TGG	0.536101	8_1:-36355566	None:intergenic
TATAATACTGAAGAACCTCT+AGG	0.593820	8_1:+36355391	Msa1191650:CDS
TTTGACACAGCTTGGCCTAG+AGG	0.596039	8_1:-36355406	None:intergenic
GCATGCTTGTAAGAACTACA+GGG	0.696465	8_1:+36355348	Msa1191650:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATAATACTGAAGAACCTCT+AGG	+	chr8_1:36355391-36355410	Msa1191650:CDS	30.0%
!	CTAAATCTTTTGACACAGCT+TGG	-	chr8_1:36355417-36355436	None:intergenic	35.0%
!	GTGTCAAAAGATTTAGAGCT+AGG	+	chr8_1:36355421-36355440	Msa1191650:CDS	35.0%
	AACATCAGTACACGATAACT+TGG	-	chr8_1:36355482-36355501	None:intergenic	35.0%
	CAGTAATCTTTGCTTGAGAA+TGG	-	chr8_1:36355536-36355555	None:intergenic	35.0%
	TGCATGCTTGTAAGAACTAC+AGG	+	chr8_1:36355347-36355366	Msa1191650:CDS	40.0%
	GCATGCTTGTAAGAACTACA+GGG	+	chr8_1:36355348-36355367	Msa1191650:CDS	40.0%
!	GTTCATCGCGATTTTTAACG+AGG	-	chr8_1:36355447-36355466	None:intergenic	40.0%
!!!	TGAGAATGGTTTTGAGAGCT+GGG	-	chr8_1:36355522-36355541	None:intergenic	40.0%
!!!	TTGAGAATGGTTTTGAGAGC+TGG	-	chr8_1:36355523-36355542	None:intergenic	40.0%
!!	TTTTGAGAGCTGGGAGAACA+AGG	-	chr8_1:36355513-36355532	None:intergenic	45.0%
	TTTGACACAGCTTGGCCTAG+AGG	-	chr8_1:36355409-36355428	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	36355346	36355576	36355346	ID=Msa1191650;Name=Msa1191650
chr8_1	mRNA	36355346	36355576	36355346	ID=Msa1191650-mRNA-1;Parent=Msa1191650;Name=Msa1191650-mRNA-1;_AED=0.55;_eAED=0.55;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|76
chr8_1	exon	36355346	36355576	36355346	ID=Msa1191650-mRNA-1:exon:11997;Parent=Msa1191650-mRNA-1
chr8_1	CDS	36355346	36355576	36355346	ID=Msa1191650-mRNA-1:cds;Parent=Msa1191650-mRNA-1

Gene Sequence	>Msa1191650 ATGCATGCTTGTAAGAACTACAGGGATTTTGATACTGCTTCTATCTATAATACTGAAGAACCTCTAGGCCAAGCTGTGTCAAAAGATTTAGAGCTAGGCCTCGTTAAAAATCGCGATGAACTGTTCGTTACTTCCAAGTTATCGTGTACTGATGTTCAACATGACCTTGTTCTCCCAGCTCTCAAAACCATTCTCAAGCAAAGATTACTGTTTGTTACTTCCAAGTTATGA
Protein sequence	>Msa1191650 MHACKNYRDFDTASIYNTEEPLGQAVSKDLELGLVKNRDELFVTSKLSCTDVQHDLVLPALKTILKQRLLFVTSKL*