AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1192780


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1192780 MtrunA17_Chr3g0120191 81.818 44 8 0 3 46 26 69 7.07e-21 80.5
Msa1192780 MtrunA17_Chr3g0120841 75.610 41 10 0 3 43 127 167 3.46e-18 78.2
Msa1192780 MtrunA17_Chr3g0120221 83.784 37 6 0 7 43 126 162 2.43e-17 73.2
Msa1192780 MtrunA17_Chr7g0239471 58.537 41 17 0 3 43 122 162 2.35e-12 61.6
Msa1192780 MtrunA17_Chr2g0290321 63.415 41 15 0 3 43 123 163 3.69e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1192780 AT4G16420.2 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1192780 AT4G16420.3 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1192780 AT4G16420.1 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1192780 AT3G07740.2 53.659 41 19 0 3 43 55 95 1.64e-11 59.3
Msa1192780 AT3G07740.3 53.659 41 19 0 3 43 105 145 1.66e-11 59.3
Msa1192780 AT3G07740.1 53.659 41 19 0 3 43 126 166 1.67e-11 59.3
Msa1192780 AT3G07740.4 53.659 41 19 0 3 43 133 173 1.67e-11 59.3

Find 22 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTAGTGGCATCATATATT+TGG 0.173555 8_1:-38737962 None:intergenic
GAACATTCCCTACAGTAAAA+AGG 0.273505 8_1:-38737837 None:intergenic
GCTATTATCATAATGGGAAT+TGG 0.363985 8_1:+38735483 Msa1192780:CDS
TGTCTAACTCTAGAATTTAG+TGG 0.369382 8_1:-38737976 None:intergenic
TTGCAGAGCTATTATCATAA+TGG 0.371408 8_1:+38735476 None:intergenic
GTCTTAAACAATATAATAGA+TGG 0.385166 8_1:-38737897 None:intergenic
TGCAGAGCTATTATCATAAT+GGG 0.426129 8_1:+38735477 None:intergenic
TGTCAATTGATACCCAAAAC+AGG 0.440009 8_1:-38735628 None:intergenic
TATTTGACACTAAATTTATG+TGG 0.491893 8_1:+38737809 Msa1192780:three_prime_UTR
AAAAGTCTAATATTAAGATC+AGG 0.519983 8_1:-38735599 None:intergenic
GCAGCAACAACTGATTCCTC+TGG 0.530634 8_1:+38737752 Msa1192780:CDS
AAAACTTAAATCTGAACAAG+AGG 0.539019 8_1:+38737999 Msa1192780:three_prime_UTR
AATGGTGTTGCTGAAAATGT+TGG 0.557196 8_1:+38735506 Msa1192780:CDS
ATGGTGTTGCTGAAAATGTT+GGG 0.565724 8_1:+38735507 Msa1192780:CDS
TATCATAATGGGAATTGGAA+TGG 0.568757 8_1:+38735488 Msa1192780:CDS
TCAGATATACAACATTATAG+TGG 0.585833 8_1:-38735556 None:intergenic
CTAATATTAAGATCAGGAAG+TGG 0.589657 8_1:-38735593 None:intergenic
AGATCAGGAAGTGGACGACA+TGG 0.615355 8_1:-38735584 None:intergenic
CTTATACTGAGATAATGTAA+CGG 0.650239 8_1:-38737782 None:intergenic
GTCAATTGATACCCAAAACA+GGG 0.663788 8_1:-38735627 None:intergenic
CTTTAGTATCGATTTCACGA+AGG 0.668239 8_1:+38736661 Msa1192780:intron
ATGTAACGGAGCATGTCCAG+AGG 0.715012 8_1:-38737768 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTGTTTTTGAA+TGG - chr8_1:38737036-38737055 None:intergenic 10.0%
!!! TTGTGTTTTTTTTTTTTTGT+GGG - chr8_1:38735794-38735813 None:intergenic 15.0%
!!! TTTGTGTTTTTTTTTTTTTG+TGG - chr8_1:38735795-38735814 None:intergenic 15.0%
!!! AGTTTGTTTTTAACAAAAAA+TGG - chr8_1:38735915-38735934 None:intergenic 15.0%
!!! TTTAGGAATTTTTGATTTAA+TGG - chr8_1:38736254-38736273 None:intergenic 15.0%
!! AAAAAAAAAGGCAAATATTT+AGG - chr8_1:38736566-38736585 None:intergenic 15.0%
!! CAAATATATAAAAAAGCTAA+AGG - chr8_1:38736770-38736789 None:intergenic 15.0%
!!! ATATCAAAATTTGTTTTTAG+AGG - chr8_1:38736932-38736951 None:intergenic 15.0%
!! ATAAGTTTAGTAATTTCTTA+AGG - chr8_1:38737129-38737148 None:intergenic 15.0%
!!! AATATCAGAGATTATTTTTT+AGG + chr8_1:38737456-38737475 Msa1192780:intron 15.0%
!! AAAAGTCTAATATTAAGATC+AGG - chr8_1:38735602-38735621 None:intergenic 20.0%
!! TTTAATAAGTTGTGATTAAC+AGG - chr8_1:38735678-38735697 None:intergenic 20.0%
!!! AAAAAATGGTGTTTTTTTTG+AGG - chr8_1:38735901-38735920 None:intergenic 20.0%
!! CTAGGTTAGAAATAAAATTT+AGG - chr8_1:38736271-38736290 None:intergenic 20.0%
!!! ATCGTTGTTTTTGAATTTTT+TGG + chr8_1:38736308-38736327 Msa1192780:intron 20.0%
!!! TCGTTGTTTTTGAATTTTTT+GGG + chr8_1:38736309-38736328 Msa1192780:intron 20.0%
!! TCAAATAAACTGTGTAATAT+TGG - chr8_1:38736387-38736406 None:intergenic 20.0%
!!! TTTGATATTTTGACATGATA+TGG + chr8_1:38736421-38736440 Msa1192780:intron 20.0%
!!! TGTAGTTTGATATTTTTTGA+AGG + chr8_1:38736461-38736480 Msa1192780:intron 20.0%
!! AATATAGGAAAAGACAATAT+AGG - chr8_1:38736822-38736841 None:intergenic 20.0%
!! ATCAAATGATGTGATAATAT+AGG - chr8_1:38736837-38736856 None:intergenic 20.0%
!! TGAATTGAATTGATTATGTT+TGG + chr8_1:38736898-38736917 Msa1192780:intron 20.0%
!!! AAAACAAATTTTGATATCAG+TGG + chr8_1:38736935-38736954 Msa1192780:intron 20.0%
!!! AAACAAATTTTGATATCAGT+GGG + chr8_1:38736936-38736955 Msa1192780:intron 20.0%
!!! CTTAAATTGATTTTACACAA+TGG + chr8_1:38737079-38737098 Msa1192780:intron 20.0%
!! TTCTTAAGGCTATTATAAAA+GGG - chr8_1:38737115-38737134 None:intergenic 20.0%
!! TTTCTTAAGGCTATTATAAA+AGG - chr8_1:38737116-38737135 None:intergenic 20.0%
!!! CAAATAAGGAATTAGTATTT+TGG + chr8_1:38737351-38737370 Msa1192780:intron 20.0%
!! TAAGTAAATCTTTCAATTGA+TGG - chr8_1:38737506-38737525 None:intergenic 20.0%
!!! GAAAAAAGGTTTTCTAATTT+TGG + chr8_1:38737543-38737562 Msa1192780:intron 20.0%
!! TATTTGACACTAAATTTATG+TGG + chr8_1:38737809-38737828 Msa1192780:three_prime_UTR 20.0%
!! GTCTTAAACAATATAATAGA+TGG - chr8_1:38737900-38737919 None:intergenic 20.0%
!!! TAATGTGCAATGTTTATTTT+TGG + chr8_1:38737920-38737939 Msa1192780:three_prime_UTR 20.0%
! TCAGATATACAACATTATAG+TGG - chr8_1:38735559-38735578 None:intergenic 25.0%
!!! TGGTTGTTGAAAGTTATTTT+AGG - chr8_1:38735716-38735735 None:intergenic 25.0%
! AAGAGGAAATATTCTTCTAT+TGG - chr8_1:38735836-38735855 None:intergenic 25.0%
! AACGATTGAAGAAAAAGTAA+GGG - chr8_1:38736294-38736313 None:intergenic 25.0%
!!! TATTTTTTGAAGGTTTGAGA+AGG + chr8_1:38736471-38736490 Msa1192780:intron 25.0%
!! TGCAAGTAAGTTCAATTTTA+TGG + chr8_1:38736523-38736542 Msa1192780:intron 25.0%
!! AAAGGCAAATATTTAGGATA+AGG - chr8_1:38736560-38736579 None:intergenic 25.0%
! TTCAAAGCTTAAACTTTCTT+AGG + chr8_1:38736607-38736626 Msa1192780:intron 25.0%
! TCAAAGCTTAAACTTTCTTA+GGG + chr8_1:38736608-38736627 Msa1192780:intron 25.0%
!! TCGTATATGTTTTTCTTTGT+AGG + chr8_1:38736638-38736657 Msa1192780:intron 25.0%
! AAAAGACAATATAGGAAAAG+AGG - chr8_1:38736814-38736833 None:intergenic 25.0%
! TTCTATATATAACGTTGTGT+TGG + chr8_1:38737212-38737231 Msa1192780:intron 25.0%
! TCCTATATAGAAGACAAATA+AGG + chr8_1:38737337-38737356 Msa1192780:intron 25.0%
!! TCCTTATTTGTCTTCTATAT+AGG - chr8_1:38737341-38737360 None:intergenic 25.0%
!!! AATCTTTCAATTGATGGTTT+TGG - chr8_1:38737500-38737519 None:intergenic 25.0%
!!! AACTGTATGATAAAGTATTG+TGG + chr8_1:38737587-38737606 Msa1192780:intron 25.0%
!!! ACTGTATGATAAAGTATTGT+GGG + chr8_1:38737588-38737607 Msa1192780:intron 25.0%
! CTTATACTGAGATAATGTAA+CGG - chr8_1:38737785-38737804 None:intergenic 25.0%
!!! TGCAATGTTTATTTTTGGAT+TGG + chr8_1:38737925-38737944 Msa1192780:three_prime_UTR 25.0%
! ATTTAGTGGCATCATATATT+TGG - chr8_1:38737965-38737984 None:intergenic 25.0%
! GCTATTATCATAATGGGAAT+TGG + chr8_1:38735483-38735502 Msa1192780:CDS 30.0%
! TATCATAATGGGAATTGGAA+TGG + chr8_1:38735488-38735507 Msa1192780:CDS 30.0%
CTAATATTAAGATCAGGAAG+TGG - chr8_1:38735596-38735615 None:intergenic 30.0%
!!! AATGGTGTTTTTTTTGAGGT+GGG - chr8_1:38735897-38735916 None:intergenic 30.0%
!!! AAATGGTGTTTTTTTTGAGG+TGG - chr8_1:38735898-38735917 None:intergenic 30.0%
ATTCAAATTTCCCCCTTAAA+AGG + chr8_1:38736035-38736054 Msa1192780:intron 30.0%
! ATGTTCGTTTGACCTTTTAA+GGG - chr8_1:38736050-38736069 None:intergenic 30.0%
GAAAGAAACAAATTGAGAGA+GGG - chr8_1:38736073-38736092 None:intergenic 30.0%
AGAAAGAAACAAATTGAGAG+AGG - chr8_1:38736074-38736093 None:intergenic 30.0%
CAACGATTGAAGAAAAAGTA+AGG - chr8_1:38736295-38736314 None:intergenic 30.0%
TGATCAGCACGAAAAAAAAA+AGG - chr8_1:38736578-38736597 None:intergenic 30.0%
CAAAGCTTAAACTTTCTTAG+GGG + chr8_1:38736609-38736628 Msa1192780:intron 30.0%
AAAGCTTAAACTTTCTTAGG+GGG + chr8_1:38736610-38736629 Msa1192780:intron 30.0%
CAACAACAACTACTAATACA+TGG - chr8_1:38736860-38736879 None:intergenic 30.0%
!!! AGTAGTTGTTGTTGTTGTTT+TGG + chr8_1:38736866-38736885 Msa1192780:intron 30.0%
!! AATTGATTTTACACAATGGC+TGG + chr8_1:38737083-38737102 Msa1192780:intron 30.0%
TGCTAAGTAACTTGATCATT+CGG + chr8_1:38737150-38737169 Msa1192780:intron 30.0%
! ACTTAGAATGTTTCTGATAG+TGG + chr8_1:38737521-38737540 Msa1192780:intron 30.0%
!! TGTTTCTGATAGTGGAAAAA+AGG + chr8_1:38737529-38737548 Msa1192780:intron 30.0%
!! CTAATTTTGGAGAAAATCGA+TGG + chr8_1:38737556-38737575 Msa1192780:intron 30.0%
!! CTGTATGATAAAGTATTGTG+GGG + chr8_1:38737589-38737608 Msa1192780:intron 30.0%
!!! TAAAGTATTGTGGGGATTTT+AGG + chr8_1:38737597-38737616 Msa1192780:intron 30.0%
!!! TTATTTTTGGATTGGTTCCT+TGG + chr8_1:38737933-38737952 Msa1192780:three_prime_UTR 30.0%
! CATATATTTGGATTTTGCCA+AGG - chr8_1:38737953-38737972 None:intergenic 30.0%
!! TGTCTAACTCTAGAATTTAG+TGG - chr8_1:38737979-38737998 None:intergenic 30.0%
!! AATGGTGTTGCTGAAAATGT+TGG + chr8_1:38735506-38735525 Msa1192780:CDS 35.0%
!! ATGGTGTTGCTGAAAATGTT+GGG + chr8_1:38735507-38735526 Msa1192780:CDS 35.0%
!!! ACTTTTTGAAGCCCTGTTTT+GGG + chr8_1:38735616-38735635 Msa1192780:CDS 35.0%
GTCAATTGATACCCAAAACA+GGG - chr8_1:38735630-38735649 None:intergenic 35.0%
TGTCAATTGATACCCAAAAC+AGG - chr8_1:38735631-38735650 None:intergenic 35.0%
! TCTTTTTCCTTCTCTCTGAA+TGG - chr8_1:38735740-38735759 None:intergenic 35.0%
GCATATACCTCAAGTGAAAT+GGG - chr8_1:38735765-38735784 None:intergenic 35.0%
AGCATATACCTCAAGTGAAA+TGG - chr8_1:38735766-38735785 None:intergenic 35.0%
AAGAAGGAAAAGAGAGAAAG+AGG - chr8_1:38735853-38735872 None:intergenic 35.0%
GGAAAAGATGAAAGCAAAGA+AGG - chr8_1:38735869-38735888 None:intergenic 35.0%
!!! ATGGTGTTTTTTTTGAGGTG+GGG - chr8_1:38735896-38735915 None:intergenic 35.0%
ACTATGTACTGTACAAACAC+AGG + chr8_1:38735932-38735951 Msa1192780:intron 35.0%
ATAGTTCAGAAATTGCAGAG+CGG - chr8_1:38736001-38736020 None:intergenic 35.0%
! TGTTCGTTTGACCTTTTAAG+GGG - chr8_1:38736049-38736068 None:intergenic 35.0%
! GATGTTCGTTTGACCTTTTA+AGG - chr8_1:38736051-38736070 None:intergenic 35.0%
! TCTGTTACTATAGATTGGTC+TGG - chr8_1:38736177-38736196 None:intergenic 35.0%
AGAGCTCTGTTACTATAGAT+TGG - chr8_1:38736182-38736201 None:intergenic 35.0%
AACAGAGCTCTTCTTCATTA+AGG + chr8_1:38736191-38736210 Msa1192780:intron 35.0%
ACAGAGCTCTTCTTCATTAA+GGG + chr8_1:38736192-38736211 Msa1192780:intron 35.0%
TCTTCTTCATTAAGGGTAAG+CGG + chr8_1:38736199-38736218 Msa1192780:intron 35.0%
TTGAAGAAAAAGTAAGGGCT+AGG - chr8_1:38736289-38736308 None:intergenic 35.0%
TACTCAAACACAGCTATACT+GGG - chr8_1:38736351-38736370 None:intergenic 35.0%
TTACTCAAACACAGCTATAC+TGG - chr8_1:38736352-38736371 None:intergenic 35.0%
AGCAACTTTACTAAGCTCAT+AGG - chr8_1:38736503-38736522 None:intergenic 35.0%
!! CTTTAGTATCGATTTCACGA+AGG + chr8_1:38736661-38736680 Msa1192780:intron 35.0%
! TGATTTTTGTTGCAAGAGCA+AGG + chr8_1:38736701-38736720 Msa1192780:CDS 35.0%
ACACTAACATAGAAGAACAG+AGG + chr8_1:38736981-38737000 Msa1192780:intron 35.0%
!! TAACTTGATCATTCGGTGTT+TGG + chr8_1:38737157-38737176 Msa1192780:intron 35.0%
AGTAGATCAGATGCATGATA+TGG - chr8_1:38737241-38737260 None:intergenic 35.0%
ATCATGTGCACAAACAAAGT+AGG - chr8_1:38737632-38737651 None:intergenic 35.0%
GAACATTCCCTACAGTAAAA+AGG - chr8_1:38737840-38737859 None:intergenic 35.0%
!!! GACTTTTTGAAGCCCTGTTT+TGG + chr8_1:38735615-38735634 Msa1192780:CDS 40.0%
TTTCCTTCTCTCTGAATGGT+TGG - chr8_1:38735736-38735755 None:intergenic 40.0%
AAAGAGACCCATTTCACTTG+AGG + chr8_1:38735755-38735774 Msa1192780:intron 40.0%
!!! TTTTTTTTGAGGTGGGGAAG+GGG - chr8_1:38735890-38735909 None:intergenic 40.0%
!!! GTTTTTTTTGAGGTGGGGAA+GGG - chr8_1:38735891-38735910 None:intergenic 40.0%
!!! TGTTTTTTTTGAGGTGGGGA+AGG - chr8_1:38735892-38735911 None:intergenic 40.0%
TAGTTCAGAAATTGCAGAGC+GGG - chr8_1:38736000-38736019 None:intergenic 40.0%
!! GTTCGTTTGACCTTTTAAGG+GGG - chr8_1:38736048-38736067 None:intergenic 40.0%
CGAAAACAAAGCCAACGAAA+AGG - chr8_1:38736141-38736160 None:intergenic 40.0%
CTAACATAGAAGAACAGAGG+AGG + chr8_1:38736984-38737003 Msa1192780:intron 40.0%
!! TCATTCGGTGTTTGGTGTAA+AGG + chr8_1:38737165-38737184 Msa1192780:intron 40.0%
TGCACAAAAGCTATGGAGAA+AGG - chr8_1:38737694-38737713 None:intergenic 40.0%
! TGGTGAAGCCTTTTTACTGT+AGG + chr8_1:38737829-38737848 Msa1192780:three_prime_UTR 40.0%
! GGTGAAGCCTTTTTACTGTA+GGG + chr8_1:38737830-38737849 Msa1192780:three_prime_UTR 40.0%
CAACCAACCATTCAGAGAGA+AGG + chr8_1:38735730-38735749 Msa1192780:intron 45.0%
!!! ACAGGCGTGAGGTTTTGTTT+TGG + chr8_1:38735950-38735969 Msa1192780:intron 45.0%
GAAGAACTCAGCAGTGAAGA+AGG - chr8_1:38736119-38736138 None:intergenic 45.0%
! GCTGAGTTCTTCCTTTTCGT+TGG + chr8_1:38736127-38736146 Msa1192780:intron 45.0%
! TCTCCATAGCTTTTGTGCAC+AGG + chr8_1:38737695-38737714 Msa1192780:intron 45.0%
TAGCCTGTGCACAAAAGCTA+TGG - chr8_1:38737701-38737720 None:intergenic 45.0%
AGATCAGGAAGTGGACGACA+TGG - chr8_1:38735587-38735606 None:intergenic 50.0%
ACTGTACAAACACAGGCGTG+AGG + chr8_1:38735939-38735958 Msa1192780:intron 50.0%
! GCAGCAACAACTGATTCCTC+TGG + chr8_1:38737752-38737771 Msa1192780:CDS 50.0%
! ATGTAACGGAGCATGTCCAG+AGG - chr8_1:38737771-38737790 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 38735482 38738020 38735482 ID=Msa1192780;Name=Msa1192780
chr8_1 mRNA 38735482 38738020 38735482 ID=Msa1192780-mRNA-1;Parent=Msa1192780;Name=Msa1192780-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0.5|0.33|0.66|0|0.33|3|217|103
chr8_1 exon 38735482 38735637 38735482 ID=Msa1192780-mRNA-1:exon:12662;Parent=Msa1192780-mRNA-1
chr8_1 exon 38736667 38736722 38736667 ID=Msa1192780-mRNA-1:exon:12663;Parent=Msa1192780-mRNA-1
chr8_1 exon 38737704 38738020 38737704 ID=Msa1192780-mRNA-1:exon:12664;Parent=Msa1192780-mRNA-1
chr8_1 CDS 38735482 38735637 38735482 ID=Msa1192780-mRNA-1:cds;Parent=Msa1192780-mRNA-1
chr8_1 CDS 38736667 38736722 38736667 ID=Msa1192780-mRNA-1:cds;Parent=Msa1192780-mRNA-1
chr8_1 CDS 38737704 38737803 38737704 ID=Msa1192780-mRNA-1:cds;Parent=Msa1192780-mRNA-1
chr8_1 three_prime_UTR 38737804 38738020 38737804 ID=Msa1192780-mRNA-1:three_prime_utr;Parent=Msa1192780-mRNA-1
Gene Sequence

>Msa1192780

AGCTATTATCATAATGGGAATTGGAATGGTGTTGCTGAAAATGTTGGGACGAAAAGCAAATCTCAATGTATTGACCACTATAATGTTGTATATCTGAATTCGCCATGTCGTCCACTTCCTGATCTTAATATTAGACTTTTTGAAGCCCTGTTTTGGTATCGATTTCACGAAGGAGTAGCTTGCTACATGCTGATTTTTGTTGCAAGAGCAAGCTTTTGTGCACAGGCTATTCATCAATTTAAGAAAGAGCAAGTTCTCTTGCAGCAACAACTGATTCCTCTGGACATGCTCCGTTACATTATCTCAGTATAA

Protein sequence

>Msa1192780

SYYHNGNWNGVAENVGTKSKSQCIDHYNVVYLNSPCRPLPDLNIRLFEALFWYRFHEGVACYMLIFVARASFCAQAIHQFKKEQVLLQQQLIPLDMLRYIISV*