AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1327390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327390 MtrunA17_Chr3g0120191 81.818 44 8 0 3 46 26 69 7.07e-21 80.5
Msa1327390 MtrunA17_Chr3g0120841 75.610 41 10 0 3 43 127 167 3.46e-18 78.2
Msa1327390 MtrunA17_Chr3g0120221 83.784 37 6 0 7 43 126 162 2.43e-17 73.2
Msa1327390 MtrunA17_Chr7g0239471 58.537 41 17 0 3 43 122 162 2.35e-12 61.6
Msa1327390 MtrunA17_Chr2g0290321 63.415 41 15 0 3 43 123 163 3.69e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1327390 AT4G16420.2 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1327390 AT4G16420.3 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1327390 AT4G16420.1 63.415 41 15 0 3 43 120 160 1.11e-12 62.8
Msa1327390 AT3G07740.2 53.659 41 19 0 3 43 55 95 1.64e-11 59.3
Msa1327390 AT3G07740.3 53.659 41 19 0 3 43 105 145 1.66e-11 59.3
Msa1327390 AT3G07740.1 53.659 41 19 0 3 43 126 166 1.67e-11 59.3
Msa1327390 AT3G07740.4 53.659 41 19 0 3 43 133 173 1.67e-11 59.3

Find 22 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTAGTGGCATCATATATT+TGG 0.173555 8_4:-44712346 None:intergenic
GAACATTCCCTACAGTAAAA+AGG 0.273505 8_4:-44712221 None:intergenic
GCTATTATCATAATGGGAAT+TGG 0.363985 8_4:+44709868 Msa1327390:CDS
TGTCTAACTCTAGAATTTAG+TGG 0.369382 8_4:-44712360 None:intergenic
TTGCAGAGCTATTATCATAA+TGG 0.371408 8_4:+44709861 None:intergenic
GTCTTAAACAATATAATAGA+TGG 0.385166 8_4:-44712281 None:intergenic
TGCAGAGCTATTATCATAAT+GGG 0.426129 8_4:+44709862 None:intergenic
TGTCAATTGATACCCAAAAC+AGG 0.440009 8_4:-44710013 None:intergenic
TATTTGACACTAAATTTATG+TGG 0.491893 8_4:+44712193 Msa1327390:three_prime_UTR
AAAAGTCTAATATTAAGATC+AGG 0.519983 8_4:-44709984 None:intergenic
GCAGCAACAACTGATTCCTC+TGG 0.530634 8_4:+44712136 Msa1327390:CDS
AAAACTTAAATCTGAACAAG+AGG 0.539019 8_4:+44712383 Msa1327390:three_prime_UTR
AATGGTGTTGCTGAAAATGT+TGG 0.557196 8_4:+44709891 Msa1327390:CDS
ATGGTGTTGCTGAAAATGTT+GGG 0.565724 8_4:+44709892 Msa1327390:CDS
TATCATAATGGGAATTGGAA+TGG 0.568757 8_4:+44709873 Msa1327390:CDS
TCAGATATACAACATTATAG+TGG 0.585833 8_4:-44709941 None:intergenic
CTAATATTAAGATCAGGAAG+TGG 0.589657 8_4:-44709978 None:intergenic
AGATCAGGAAGTGGACGACA+TGG 0.615355 8_4:-44709969 None:intergenic
CTTATACTGAGATAATGTAA+CGG 0.650239 8_4:-44712166 None:intergenic
GTCAATTGATACCCAAAACA+GGG 0.663788 8_4:-44710012 None:intergenic
CTTTAGTATCGATTTCACGA+AGG 0.668239 8_4:+44711046 Msa1327390:intron
ATGTAACGGAGCATGTCCAG+AGG 0.715012 8_4:-44712152 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTGTTTTTGAA+TGG - chr8_4:44711421-44711440 None:intergenic 10.0%
!!! TGTGTTTTTTTTTTTTTTGT+GGG - chr8_4:44710178-44710197 None:intergenic 15.0%
!!! TTGTGTTTTTTTTTTTTTTG+TGG - chr8_4:44710179-44710198 None:intergenic 15.0%
!!! AGTTTGTTTTTAACAAAAAA+TGG - chr8_4:44710300-44710319 None:intergenic 15.0%
!!! TTTAGGAATTTTTGATTTAA+TGG - chr8_4:44710639-44710658 None:intergenic 15.0%
!! AAAAAAAAAGGCAAATATTT+AGG - chr8_4:44710951-44710970 None:intergenic 15.0%
!! CAAATATATAAAAAAGCTAA+AGG - chr8_4:44711155-44711174 None:intergenic 15.0%
!!! ATATCAAAATTTGTTTTTAG+AGG - chr8_4:44711317-44711336 None:intergenic 15.0%
!! ATAAGTTTAGTAATTTCTTA+AGG - chr8_4:44711514-44711533 None:intergenic 15.0%
!!! AATATCAGAGATTATTTTTT+AGG + chr8_4:44711840-44711859 Msa1327390:intron 15.0%
!! AAAAGTCTAATATTAAGATC+AGG - chr8_4:44709987-44710006 None:intergenic 20.0%
!! TTTAATAAGTTGTGATTAAC+AGG - chr8_4:44710063-44710082 None:intergenic 20.0%
!!! AAAAAATGGTGTTTTTTTTG+AGG - chr8_4:44710286-44710305 None:intergenic 20.0%
!! CTAGGTTAGAAATAAAATTT+AGG - chr8_4:44710656-44710675 None:intergenic 20.0%
!!! ATCGTTGTTTTTGAATTTTT+TGG + chr8_4:44710693-44710712 Msa1327390:intron 20.0%
!!! TCGTTGTTTTTGAATTTTTT+GGG + chr8_4:44710694-44710713 Msa1327390:intron 20.0%
!! TCAAATAAACTGTGTAATAT+TGG - chr8_4:44710772-44710791 None:intergenic 20.0%
!!! TTTGATATTTTGACATGATA+TGG + chr8_4:44710806-44710825 Msa1327390:intron 20.0%
!!! TGTAGTTTGATATTTTTTGA+AGG + chr8_4:44710846-44710865 Msa1327390:intron 20.0%
!! AATATAGGAAAAGACAATAT+AGG - chr8_4:44711207-44711226 None:intergenic 20.0%
!! ATCAAATGATGTGATAATAT+AGG - chr8_4:44711222-44711241 None:intergenic 20.0%
!! TGAATTGAATTGATTATGTT+TGG + chr8_4:44711283-44711302 Msa1327390:intron 20.0%
!!! AAAACAAATTTTGATATCAG+TGG + chr8_4:44711320-44711339 Msa1327390:intron 20.0%
!!! AAACAAATTTTGATATCAGT+GGG + chr8_4:44711321-44711340 Msa1327390:intron 20.0%
!!! CTTAAATTGATTTTACACAA+TGG + chr8_4:44711464-44711483 Msa1327390:intron 20.0%
!! TTCTTAAGGCTATTATAAAA+GGG - chr8_4:44711500-44711519 None:intergenic 20.0%
!! TTTCTTAAGGCTATTATAAA+AGG - chr8_4:44711501-44711520 None:intergenic 20.0%
!!! CAAATAAGGAATTAGTATTT+TGG + chr8_4:44711736-44711755 Msa1327390:intron 20.0%
!! TAAGTAAATCTTTCAATTGA+TGG - chr8_4:44711890-44711909 None:intergenic 20.0%
!!! GAAAAAAGGTTTTCTAATTT+TGG + chr8_4:44711927-44711946 Msa1327390:intron 20.0%
!! TATTTGACACTAAATTTATG+TGG + chr8_4:44712193-44712212 Msa1327390:three_prime_UTR 20.0%
!! GTCTTAAACAATATAATAGA+TGG - chr8_4:44712284-44712303 None:intergenic 20.0%
!!! TAATGTGCAATGTTTATTTT+TGG + chr8_4:44712304-44712323 Msa1327390:three_prime_UTR 20.0%
! TCAGATATACAACATTATAG+TGG - chr8_4:44709944-44709963 None:intergenic 25.0%
!!! TGGTTGTTGAAAGTTATTTT+AGG - chr8_4:44710100-44710119 None:intergenic 25.0%
! AAGAGGAAATATTCTTCTAT+TGG - chr8_4:44710221-44710240 None:intergenic 25.0%
! AACGATTGAAGAAAAAGTAA+GGG - chr8_4:44710679-44710698 None:intergenic 25.0%
!!! TATTTTTTGAAGGTTTGAGA+AGG + chr8_4:44710856-44710875 Msa1327390:intron 25.0%
!! TGCAAGTAAGTTCAATTTTA+TGG + chr8_4:44710908-44710927 Msa1327390:intron 25.0%
!! AAAGGCAAATATTTAGGATA+AGG - chr8_4:44710945-44710964 None:intergenic 25.0%
! TTCAAAGCTTAAACTTTCTT+AGG + chr8_4:44710992-44711011 Msa1327390:intron 25.0%
! TCAAAGCTTAAACTTTCTTA+GGG + chr8_4:44710993-44711012 Msa1327390:intron 25.0%
!! TCGTATATGTTTTTCTTTGT+AGG + chr8_4:44711023-44711042 Msa1327390:intron 25.0%
! AAAAGACAATATAGGAAAAG+AGG - chr8_4:44711199-44711218 None:intergenic 25.0%
! TTCTATATATAACGTTGTGT+TGG + chr8_4:44711597-44711616 Msa1327390:intron 25.0%
! TCCTATATAGAAGACAAATA+AGG + chr8_4:44711722-44711741 Msa1327390:intron 25.0%
!! TCCTTATTTGTCTTCTATAT+AGG - chr8_4:44711726-44711745 None:intergenic 25.0%
!!! AATCTTTCAATTGATGGTTT+TGG - chr8_4:44711884-44711903 None:intergenic 25.0%
!!! AACTGTATGATAAAGTATTG+TGG + chr8_4:44711971-44711990 Msa1327390:intron 25.0%
!!! ACTGTATGATAAAGTATTGT+GGG + chr8_4:44711972-44711991 Msa1327390:intron 25.0%
! CTTATACTGAGATAATGTAA+CGG - chr8_4:44712169-44712188 None:intergenic 25.0%
!!! TGCAATGTTTATTTTTGGAT+TGG + chr8_4:44712309-44712328 Msa1327390:three_prime_UTR 25.0%
! ATTTAGTGGCATCATATATT+TGG - chr8_4:44712349-44712368 None:intergenic 25.0%
! GCTATTATCATAATGGGAAT+TGG + chr8_4:44709868-44709887 Msa1327390:CDS 30.0%
! TATCATAATGGGAATTGGAA+TGG + chr8_4:44709873-44709892 Msa1327390:CDS 30.0%
CTAATATTAAGATCAGGAAG+TGG - chr8_4:44709981-44710000 None:intergenic 30.0%
!!! AATGGTGTTTTTTTTGAGGT+GGG - chr8_4:44710282-44710301 None:intergenic 30.0%
!!! AAATGGTGTTTTTTTTGAGG+TGG - chr8_4:44710283-44710302 None:intergenic 30.0%
ATTCAAATTTCCCCCTTAAA+AGG + chr8_4:44710420-44710439 Msa1327390:intron 30.0%
! ATGTTCGTTTGACCTTTTAA+GGG - chr8_4:44710435-44710454 None:intergenic 30.0%
GAAAGAAACAAATTGAGAGA+GGG - chr8_4:44710458-44710477 None:intergenic 30.0%
AGAAAGAAACAAATTGAGAG+AGG - chr8_4:44710459-44710478 None:intergenic 30.0%
CAACGATTGAAGAAAAAGTA+AGG - chr8_4:44710680-44710699 None:intergenic 30.0%
TGATCAGCACGAAAAAAAAA+AGG - chr8_4:44710963-44710982 None:intergenic 30.0%
CAAAGCTTAAACTTTCTTAG+GGG + chr8_4:44710994-44711013 Msa1327390:intron 30.0%
AAAGCTTAAACTTTCTTAGG+GGG + chr8_4:44710995-44711014 Msa1327390:intron 30.0%
CAACAACAACTACTAATACA+TGG - chr8_4:44711245-44711264 None:intergenic 30.0%
!!! AGTAGTTGTTGTTGTTGTTT+TGG + chr8_4:44711251-44711270 Msa1327390:intron 30.0%
!! AATTGATTTTACACAATGGC+TGG + chr8_4:44711468-44711487 Msa1327390:intron 30.0%
TGCTAAGTAACTTGATCATT+CGG + chr8_4:44711535-44711554 Msa1327390:intron 30.0%
! ACTTAGAATGTTTCTGATAG+TGG + chr8_4:44711905-44711924 Msa1327390:intron 30.0%
!! TGTTTCTGATAGTGGAAAAA+AGG + chr8_4:44711913-44711932 Msa1327390:intron 30.0%
!! CTAATTTTGGAGAAAATCGA+TGG + chr8_4:44711940-44711959 Msa1327390:intron 30.0%
!! CTGTATGATAAAGTATTGTG+GGG + chr8_4:44711973-44711992 Msa1327390:intron 30.0%
!!! TAAAGTATTGTGGGGATTTT+AGG + chr8_4:44711981-44712000 Msa1327390:intron 30.0%
!!! TTATTTTTGGATTGGTTCCT+TGG + chr8_4:44712317-44712336 Msa1327390:three_prime_UTR 30.0%
! CATATATTTGGATTTTGCCA+AGG - chr8_4:44712337-44712356 None:intergenic 30.0%
!! TGTCTAACTCTAGAATTTAG+TGG - chr8_4:44712363-44712382 None:intergenic 30.0%
!! AATGGTGTTGCTGAAAATGT+TGG + chr8_4:44709891-44709910 Msa1327390:CDS 35.0%
!! ATGGTGTTGCTGAAAATGTT+GGG + chr8_4:44709892-44709911 Msa1327390:CDS 35.0%
!!! ACTTTTTGAAGCCCTGTTTT+GGG + chr8_4:44710001-44710020 Msa1327390:CDS 35.0%
GTCAATTGATACCCAAAACA+GGG - chr8_4:44710015-44710034 None:intergenic 35.0%
TGTCAATTGATACCCAAAAC+AGG - chr8_4:44710016-44710035 None:intergenic 35.0%
! TCTTTTTCCTTCTCTCTGAA+TGG - chr8_4:44710124-44710143 None:intergenic 35.0%
GCATATACCTCAAGTGAAAT+GGG - chr8_4:44710149-44710168 None:intergenic 35.0%
AGCATATACCTCAAGTGAAA+TGG - chr8_4:44710150-44710169 None:intergenic 35.0%
AAGAAGGAAAAGAGAGAAAG+AGG - chr8_4:44710238-44710257 None:intergenic 35.0%
GGAAAAGATGAAAGCAAAGA+AGG - chr8_4:44710254-44710273 None:intergenic 35.0%
!!! ATGGTGTTTTTTTTGAGGTG+GGG - chr8_4:44710281-44710300 None:intergenic 35.0%
ACTATGTACTGTACAAACAC+AGG + chr8_4:44710317-44710336 Msa1327390:intron 35.0%
ATAGTTCAGAAATTGCAGAG+CGG - chr8_4:44710386-44710405 None:intergenic 35.0%
! TGTTCGTTTGACCTTTTAAG+GGG - chr8_4:44710434-44710453 None:intergenic 35.0%
! GATGTTCGTTTGACCTTTTA+AGG - chr8_4:44710436-44710455 None:intergenic 35.0%
! TCTGTTACTATAGATTGGTC+TGG - chr8_4:44710562-44710581 None:intergenic 35.0%
AGAGCTCTGTTACTATAGAT+TGG - chr8_4:44710567-44710586 None:intergenic 35.0%
AACAGAGCTCTTCTTCATTA+AGG + chr8_4:44710576-44710595 Msa1327390:intron 35.0%
ACAGAGCTCTTCTTCATTAA+GGG + chr8_4:44710577-44710596 Msa1327390:intron 35.0%
TCTTCTTCATTAAGGGTAAG+CGG + chr8_4:44710584-44710603 Msa1327390:intron 35.0%
TTGAAGAAAAAGTAAGGGCT+AGG - chr8_4:44710674-44710693 None:intergenic 35.0%
TACTCAAACACAGCTATACT+GGG - chr8_4:44710736-44710755 None:intergenic 35.0%
TTACTCAAACACAGCTATAC+TGG - chr8_4:44710737-44710756 None:intergenic 35.0%
AGCAACTTTACTAAGCTCAT+AGG - chr8_4:44710888-44710907 None:intergenic 35.0%
!! CTTTAGTATCGATTTCACGA+AGG + chr8_4:44711046-44711065 Msa1327390:intron 35.0%
! TGATTTTTGTTGCAAGAGCA+AGG + chr8_4:44711086-44711105 Msa1327390:CDS 35.0%
ACACTAACATAGAAGAACAG+AGG + chr8_4:44711366-44711385 Msa1327390:intron 35.0%
!! TAACTTGATCATTCGGTGTT+TGG + chr8_4:44711542-44711561 Msa1327390:intron 35.0%
AGTAGATCAGATGCATGATA+TGG - chr8_4:44711626-44711645 None:intergenic 35.0%
ATCATGTGCACAAACAAAGT+AGG - chr8_4:44712016-44712035 None:intergenic 35.0%
GAACATTCCCTACAGTAAAA+AGG - chr8_4:44712224-44712243 None:intergenic 35.0%
!!! GACTTTTTGAAGCCCTGTTT+TGG + chr8_4:44710000-44710019 Msa1327390:CDS 40.0%
TTTCCTTCTCTCTGAATGGT+TGG - chr8_4:44710120-44710139 None:intergenic 40.0%
AAAGAGACCCATTTCACTTG+AGG + chr8_4:44710139-44710158 Msa1327390:intron 40.0%
!!! TTTTTTTTGAGGTGGGGAAG+GGG - chr8_4:44710275-44710294 None:intergenic 40.0%
!!! GTTTTTTTTGAGGTGGGGAA+GGG - chr8_4:44710276-44710295 None:intergenic 40.0%
!!! TGTTTTTTTTGAGGTGGGGA+AGG - chr8_4:44710277-44710296 None:intergenic 40.0%
TAGTTCAGAAATTGCAGAGC+GGG - chr8_4:44710385-44710404 None:intergenic 40.0%
!! GTTCGTTTGACCTTTTAAGG+GGG - chr8_4:44710433-44710452 None:intergenic 40.0%
CGAAAACAAAGCCAACGAAA+AGG - chr8_4:44710526-44710545 None:intergenic 40.0%
CTAACATAGAAGAACAGAGG+AGG + chr8_4:44711369-44711388 Msa1327390:intron 40.0%
!! TCATTCGGTGTTTGGTGTAA+AGG + chr8_4:44711550-44711569 Msa1327390:intron 40.0%
TGCACAAAAGCTATGGAGAA+AGG - chr8_4:44712078-44712097 None:intergenic 40.0%
! TGGTGAAGCCTTTTTACTGT+AGG + chr8_4:44712213-44712232 Msa1327390:three_prime_UTR 40.0%
! GGTGAAGCCTTTTTACTGTA+GGG + chr8_4:44712214-44712233 Msa1327390:three_prime_UTR 40.0%
CAACCAACCATTCAGAGAGA+AGG + chr8_4:44710114-44710133 Msa1327390:intron 45.0%
!!! ACAGGCGTGAGGTTTTGTTT+TGG + chr8_4:44710335-44710354 Msa1327390:intron 45.0%
GAAGAACTCAGCAGTGAAGA+AGG - chr8_4:44710504-44710523 None:intergenic 45.0%
! GCTGAGTTCTTCCTTTTCGT+TGG + chr8_4:44710512-44710531 Msa1327390:intron 45.0%
! TCTCCATAGCTTTTGTGCAC+AGG + chr8_4:44712079-44712098 Msa1327390:intron 45.0%
TAGCCTGTGCACAAAAGCTA+TGG - chr8_4:44712085-44712104 None:intergenic 45.0%
AGATCAGGAAGTGGACGACA+TGG - chr8_4:44709972-44709991 None:intergenic 50.0%
ACTGTACAAACACAGGCGTG+AGG + chr8_4:44710324-44710343 Msa1327390:intron 50.0%
! GCAGCAACAACTGATTCCTC+TGG + chr8_4:44712136-44712155 Msa1327390:CDS 50.0%
! ATGTAACGGAGCATGTCCAG+AGG - chr8_4:44712155-44712174 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 44709867 44712404 44709867 ID=Msa1327390;Name=Msa1327390
chr8_4 mRNA 44709867 44712404 44709867 ID=Msa1327390-mRNA-1;Parent=Msa1327390;Name=Msa1327390-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|0.5|0.33|0.66|0|0.33|3|217|103
chr8_4 exon 44709867 44710022 44709867 ID=Msa1327390-mRNA-1:exon:12469;Parent=Msa1327390-mRNA-1
chr8_4 exon 44711052 44711107 44711052 ID=Msa1327390-mRNA-1:exon:12470;Parent=Msa1327390-mRNA-1
chr8_4 exon 44712088 44712404 44712088 ID=Msa1327390-mRNA-1:exon:12471;Parent=Msa1327390-mRNA-1
chr8_4 CDS 44709867 44710022 44709867 ID=Msa1327390-mRNA-1:cds;Parent=Msa1327390-mRNA-1
chr8_4 CDS 44711052 44711107 44711052 ID=Msa1327390-mRNA-1:cds;Parent=Msa1327390-mRNA-1
chr8_4 CDS 44712088 44712187 44712088 ID=Msa1327390-mRNA-1:cds;Parent=Msa1327390-mRNA-1
chr8_4 three_prime_UTR 44712188 44712404 44712188 ID=Msa1327390-mRNA-1:three_prime_utr;Parent=Msa1327390-mRNA-1
Gene Sequence

>Msa1327390

AGCTATTATCATAATGGGAATTGGAATGGTGTTGCTGAAAATGTTGGGACGAAAAGCAAATCTCAATGTATTGACCACTATAATGTTGTATATCTGAATTCGCCATGTCGTCCACTTCCTGATCTTAATATTAGACTTTTTGAAGCCCTGTTTTGGTATCGATTTCACGAAGGAGTAGCTTGCTACATGCTGATTTTTGTTGCAAGAGCAAGCTTTTGTGCACAGGCTATTCATCAATTTAAGAAAGAGCAAGTTCTCTTGCAGCAACAACTGATTCCTCTGGACATGCTCCGTTACATTATCTCAGTATAA

Protein sequence

>Msa1327390

SYYHNGNWNGVAENVGTKSKSQCIDHYNVVYLNSPCRPLPDLNIRLFEALFWYRFHEGVACYMLIFVARASFCAQAIHQFKKEQVLLQQQLIPLDMLRYIISV*