AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1195020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1195020 MtrunA17_Chr8g0363921 95.789 285 4 3 1 284 1 278 0.0 545
Msa1195020 MtrunA17_Chr5g0415291 70.046 217 45 6 74 284 37 239 8.54e-96 281
Msa1195020 MtrunA17_Chr7g0234761 53.659 205 73 6 83 275 234 428 1.63e-52 179
Msa1195020 MtrunA17_Chr8g0339091 56.098 164 61 3 83 238 212 372 4.25e-51 175
Msa1195020 MtrunA17_Chr5g0421111 71.930 114 30 1 133 246 343 454 1.97e-47 166
Msa1195020 MtrunA17_Chr8g0365941 54.118 170 68 4 78 246 234 394 4.69e-47 163
Msa1195020 MtrunA17_Chr1g0152131 38.721 297 126 10 5 256 74 359 1.74e-46 161
Msa1195020 MtrunA17_Chr4g0035101 55.484 155 53 2 109 250 85 236 4.66e-45 155
Msa1195020 MtrunA17_Chr3g0144641 60.417 144 44 4 122 253 150 292 7.08e-45 156
Msa1195020 MtrunA17_Chr8g0385911 88.000 75 9 0 154 228 142 216 3.33e-40 142
Msa1195020 MtrunA17_Chr3g0131771 82.716 81 14 0 148 228 138 218 8.83e-40 141
Msa1195020 MtrunA17_Chr7g0258161 72.093 86 24 0 151 236 167 252 1.40e-37 135
Msa1195020 MtrunA17_Chr1g0174161 69.412 85 26 0 154 238 157 241 4.13e-36 131
Msa1195020 MtrunA17_Chr6g0484271 50.000 140 53 3 98 237 113 235 8.78e-34 124
Msa1195020 MtrunA17_Chr4g0074161 59.756 82 32 1 141 222 190 270 4.26e-22 95.1
Msa1195020 MtrunA17_Chr1g0190731 58.974 78 26 2 145 222 186 257 6.12e-20 88.2
Msa1195020 MtrunA17_Chr1g0182791 47.561 82 41 2 159 240 319 398 2.87e-15 75.5
Msa1195020 MtrunA17_Chr2g0329041 38.194 144 72 5 87 219 257 394 3.89e-15 75.1
Msa1195020 MtrunA17_Chr1g0193321 50.000 64 31 1 159 222 464 526 4.52e-15 75.1
Msa1195020 MtrunA17_Chr4g0032951 55.385 65 29 0 157 221 355 419 1.60e-14 73.2
Msa1195020 MtrunA17_Chr1g0174271 48.101 79 36 1 145 223 291 364 1.27e-13 70.5
Msa1195020 MtrunA17_Chr4g0014771 49.231 65 33 0 157 221 118 182 3.63e-13 66.6
Msa1195020 MtrunA17_Chr1g0184841 60.606 66 25 1 157 222 120 184 4.89e-13 67.8
Msa1195020 MtrunA17_Chr7g0258181 44.595 74 41 0 157 230 243 316 4.91e-13 68.2
Msa1195020 MtrunA17_Chr1g0152651 49.231 65 32 1 157 221 274 337 1.93e-12 66.6
Msa1195020 MtrunA17_Chr1g0153241 36.364 110 50 4 114 223 61 150 3.83e-12 65.1
Msa1195020 MtrunA17_Chr3g0134741 35.659 129 69 3 106 221 165 292 4.30e-12 65.5
Msa1195020 MtrunA17_Chr5g0393721 26.991 226 131 6 29 221 63 287 7.68e-12 64.7
Msa1195020 MtrunA17_Chr8g0351881 47.692 65 34 0 157 221 316 380 7.83e-12 65.1
Msa1195020 MtrunA17_Chr7g0262691 46.875 64 33 1 159 222 254 316 1.29e-11 64.7
Msa1195020 MtrunA17_Chr7g0271251 48.438 64 32 1 159 222 434 496 1.88e-11 64.3
Msa1195020 MtrunA17_Chr3g0095391 46.875 64 33 1 159 222 311 373 2.87e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1195020 AT1G59640.1 57.877 292 87 10 1 284 1 264 2.03e-96 285
Msa1195020 AT1G59640.2 56.627 249 73 9 1 242 1 221 3.78e-75 233
Msa1195020 AT5G62610.2 52.041 294 118 12 1 284 1 281 3.92e-72 223
Msa1195020 AT5G62610.1 52.041 294 118 12 1 284 1 281 3.92e-72 223
Msa1195020 AT3G07340.2 58.125 160 45 3 83 242 206 343 6.25e-49 168
Msa1195020 AT3G07340.1 58.125 160 45 3 83 242 206 343 1.09e-48 168
Msa1195020 AT5G48560.2 77.451 102 19 3 142 242 289 387 7.71e-46 160
Msa1195020 AT1G26260.3 55.696 158 64 2 91 247 110 262 8.54e-46 157
Msa1195020 AT5G48560.1 77.451 102 19 3 142 242 289 387 1.06e-45 160
Msa1195020 AT1G26260.5 55.696 158 64 2 91 247 139 291 1.41e-45 157
Msa1195020 AT1G26260.4 55.696 158 64 2 91 247 161 313 2.41e-45 157
Msa1195020 AT1G26260.1 55.696 158 64 2 91 247 161 313 2.41e-45 157
Msa1195020 AT1G26260.6 55.696 158 64 2 91 247 161 313 2.41e-45 157
Msa1195020 AT1G26260.2 55.696 158 64 2 91 247 161 313 2.41e-45 157
Msa1195020 AT1G68920.3 75.758 99 24 0 147 245 292 390 5.80e-45 159
Msa1195020 AT1G68920.1 75.758 99 24 0 147 245 293 391 6.41e-45 158
Msa1195020 AT1G68920.4 75.758 99 24 0 147 245 293 391 6.41e-45 158
Msa1195020 AT1G68920.2 75.758 99 24 0 147 245 293 391 6.41e-45 158
Msa1195020 AT3G23690.1 61.029 136 42 3 96 231 141 265 8.69e-44 153
Msa1195020 AT5G50915.3 56.115 139 49 3 92 228 79 207 1.00e-42 147
Msa1195020 AT5G50915.4 56.115 139 49 3 92 228 79 207 1.00e-42 147
Msa1195020 AT5G50915.1 56.522 138 48 3 92 227 79 206 1.84e-42 147
Msa1195020 AT5G50915.2 56.522 138 48 3 92 227 79 206 1.84e-42 147
Msa1195020 AT1G10120.2 70.833 96 28 0 150 245 199 294 2.74e-42 149
Msa1195020 AT1G10120.1 70.833 96 28 0 150 245 199 294 2.74e-42 149
Msa1195020 AT4G36540.1 52.740 146 67 1 100 243 82 227 8.51e-42 146
Msa1195020 AT4G36540.2 53.472 144 65 1 100 241 82 225 2.06e-41 145
Msa1195020 AT2G18300.2 70.103 97 29 0 147 243 175 271 7.47e-41 144
Msa1195020 AT4G34530.1 82.500 80 14 0 151 230 166 245 1.45e-40 144
Msa1195020 AT2G18300.3 70.103 97 29 0 147 243 175 271 1.56e-40 144
Msa1195020 AT2G18300.1 71.579 95 27 0 147 241 175 269 2.14e-40 143
Msa1195020 AT2G42300.4 69.318 88 27 0 151 238 179 266 2.82e-38 136
Msa1195020 AT3G57800.2 71.591 88 25 0 151 238 198 285 8.43e-38 137
Msa1195020 AT2G42300.1 65.957 94 32 0 151 244 179 272 1.23e-37 135
Msa1195020 AT1G25330.1 48.507 134 60 2 114 245 66 192 3.59e-34 124
Msa1195020 AT1G18400.2 74.667 75 19 0 155 229 143 217 9.04e-34 123
Msa1195020 AT1G18400.1 74.667 75 19 0 155 229 143 217 1.54e-33 123
Msa1195020 AT1G73830.2 69.512 82 25 0 156 237 146 227 2.66e-33 122
Msa1195020 AT1G73830.1 69.512 82 25 0 156 237 146 227 2.93e-33 122
Msa1195020 AT3G57800.1 45.390 141 30 1 151 244 198 338 5.63e-29 114
Msa1195020 AT2G24260.2 63.014 73 26 1 150 222 28 99 1.79e-22 94.0
Msa1195020 AT2G24260.1 63.014 73 26 1 150 222 131 202 1.30e-21 93.2
Msa1195020 AT4G30980.1 68.182 66 20 1 157 222 130 194 5.90e-21 90.9
Msa1195020 AT4G30980.2 68.182 66 20 1 157 222 178 242 8.14e-21 91.3
Msa1195020 AT5G58010.1 61.842 76 28 1 147 222 89 163 8.64e-21 90.1
Msa1195020 AT2G42300.5 78.723 47 10 0 151 197 179 225 2.80e-19 84.7
Msa1195020 AT2G42300.3 78.723 47 10 0 151 197 179 225 2.80e-19 84.7
Msa1195020 AT2G42300.2 80.435 46 9 0 151 196 179 224 3.46e-19 84.7
Msa1195020 AT4G02590.3 49.515 103 46 3 125 222 46 147 1.50e-15 74.7
Msa1195020 AT4G02590.2 49.515 103 46 3 125 222 109 210 2.27e-15 75.1
Msa1195020 AT4G02590.1 49.515 103 46 3 125 222 109 210 2.27e-15 75.1
Msa1195020 AT2G20180.7 51.562 64 30 1 159 222 209 271 5.81e-15 74.7
Msa1195020 AT2G20180.1 51.562 64 30 1 159 222 209 271 5.81e-15 74.7
Msa1195020 AT2G20180.5 51.562 64 30 1 159 222 209 271 5.81e-15 74.7
Msa1195020 AT2G20180.4 51.562 64 30 1 159 222 280 342 7.46e-15 74.7
Msa1195020 AT2G20180.6 51.562 64 30 1 159 222 280 342 7.46e-15 74.7
Msa1195020 AT2G20180.2 51.562 64 30 1 159 222 280 342 7.46e-15 74.7
Msa1195020 AT2G20180.3 51.562 64 30 1 159 222 280 342 7.46e-15 74.7
Msa1195020 AT1G09530.3 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G09530.2 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G09530.5 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G09530.1 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G09530.4 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G09530.6 50.000 64 31 1 159 222 339 401 8.81e-15 74.7
Msa1195020 AT1G03040.1 48.544 103 47 3 125 222 107 208 2.12e-14 72.4
Msa1195020 AT1G03040.3 48.544 103 47 3 125 222 107 208 2.53e-14 72.4
Msa1195020 AT4G28800.1 44.000 75 41 1 160 234 252 325 1.64e-13 70.5
Msa1195020 AT1G03040.2 54.217 83 35 2 142 222 122 203 2.41e-13 69.3
Msa1195020 AT2G20180.8 50.820 61 29 1 159 219 280 339 3.41e-13 69.3
Msa1195020 AT2G42280.1 50.000 64 32 0 157 220 279 342 4.00e-13 69.3
Msa1195020 AT1G51140.1 53.125 64 30 0 157 220 304 367 4.47e-13 68.9
Msa1195020 AT4G28815.2 51.724 58 27 1 160 217 71 127 5.10e-13 67.4
Msa1195020 AT4G09180.1 46.269 67 36 0 155 221 182 248 8.13e-13 67.4
Msa1195020 AT2G42280.3 51.515 66 30 1 157 220 279 344 8.94e-13 68.2
Msa1195020 AT4G28800.2 45.833 72 37 2 160 230 264 334 1.25e-12 68.2
Msa1195020 AT4G28790.1 35.200 125 61 5 159 279 273 381 1.38e-12 67.8
Msa1195020 AT4G28815.1 51.724 58 27 1 160 217 147 203 1.54e-12 67.0
Msa1195020 AT2G46970.1 43.836 73 35 2 155 222 216 287 2.35e-12 67.0
Msa1195020 AT2G46970.2 43.836 73 35 2 155 222 185 256 2.64e-12 67.0
Msa1195020 AT2G46970.3 43.836 73 35 2 155 222 194 265 2.78e-12 67.0
Msa1195020 AT4G33880.1 42.857 84 45 2 140 221 247 329 3.91e-12 66.2
Msa1195020 AT1G35460.1 46.154 65 35 0 157 221 181 245 5.54e-12 65.1
Msa1195020 AT4G00050.2 47.692 65 33 1 157 221 105 168 1.43e-11 64.3
Msa1195020 AT1G27740.1 49.231 65 32 1 157 221 167 230 2.04e-11 63.2
Msa1195020 AT2G14760.1 49.231 65 32 1 157 221 237 300 2.30e-11 63.9
Msa1195020 AT4G00050.1 47.692 65 33 1 157 221 207 270 2.47e-11 63.9
Msa1195020 AT4G00050.3 47.692 65 33 1 157 221 207 270 2.88e-11 63.9
Msa1195020 AT4G36930.1 47.541 61 31 1 163 223 197 256 8.80e-11 62.4

Find 119 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTTCAATTTAGGTTAT+TGG 0.179282 8_1:+42751976 Msa1195020:intron
CCATACCTTTGTGTAGAAAA+TGG 0.194010 8_1:-42753044 None:intergenic
GTGTACTATAGTGGACTTTC+AGG 0.196499 8_1:+42753140 Msa1195020:three_prime_UTR
TGCTTAACACTTACATCTTT+TGG 0.201350 8_1:-42752214 None:intergenic
TTTCTTCAATTTAGGTTATT+GGG 0.256415 8_1:+42751977 Msa1195020:intron
CCGCAGTTTGGTAACGGTTT+AGG 0.282346 8_1:+42750283 Msa1195020:CDS
CAGTTTCCAGCGGCGATTAA+TGG 0.284846 8_1:+42750235 Msa1195020:CDS
TTTAGTTCCTGGTTGTAATA+AGG 0.288451 8_1:+42751757 Msa1195020:CDS
AGTTGAAACCGGTTCGGTTC+GGG 0.304126 8_1:+42750108 Msa1195020:five_prime_UTR
GGCATTGGCACCGCCATTAC+TGG 0.323699 8_1:-42750443 None:intergenic
CTCAATATTGTGTACTATAG+TGG 0.335669 8_1:+42753131 Msa1195020:three_prime_UTR
ATTCTACAGGATTTAGTTCC+TGG 0.338943 8_1:+42751746 Msa1195020:CDS
TTAGGACAGTTTGGTGAGTT+TGG 0.345423 8_1:+42750301 Msa1195020:CDS
AGAGAGAGAGAAAGAGTGTT+AGG 0.354588 8_1:-42749970 None:intergenic
CAACAGGCTGCTTCAATTTG+TGG 0.357110 8_1:+42753082 Msa1195020:three_prime_UTR
GAGTTGAAACCGGTTCGGTT+CGG 0.365096 8_1:+42750107 Msa1195020:five_prime_UTR
CATCGCTCCTGGATCCATTT+CGG 0.370295 8_1:-42750146 None:intergenic
CGTTTAGTTTAGCGGAGATC+TGG 0.375505 8_1:+42750212 Msa1195020:CDS
CAACAAACGTTTGATCTTGC+CGG 0.377074 8_1:+42752784 Msa1195020:CDS
ATTAATGGCGGAGGTGGATT+GGG 0.380316 8_1:+42750250 Msa1195020:CDS
TTGAAGTTATTGAACCGAAA+TGG 0.383029 8_1:+42750132 Msa1195020:exon
ATTACCATCCCGGTTTAAAC+CGG 0.385440 8_1:-42750326 None:intergenic
ACCTGCATATGTAACCATTC+TGG 0.392490 8_1:-42752856 None:intergenic
TGGACAATAAACAGGCTAGA+AGG 0.396885 8_1:+42751699 Msa1195020:intron
CCCTCGTTCATCATCGCTCC+TGG 0.402780 8_1:-42750157 None:intergenic
ACATCCATGTTCGAGCTAGA+AGG 0.403285 8_1:+42751335 Msa1195020:CDS
ACTTCAAACCCGAACCGAAC+CGG 0.408133 8_1:-42750116 None:intergenic
CAGATCTCCGCTAAACTAAA+CGG 0.416576 8_1:-42750211 None:intergenic
GTTGAAGGAGAAGTCAGTTC+AGG 0.418689 8_1:+42751256 Msa1195020:CDS
GGAGCATTTGAAGGATGAAA+TGG 0.421937 8_1:-42752959 None:intergenic
CATACCTTTGTGTAGAAAAT+GGG 0.425858 8_1:-42753043 None:intergenic
AGTTTGGACCCGGTTTAAAC+CGG 0.426792 8_1:+42750317 Msa1195020:CDS
ACGAGGGTTCGTTTCCGAAT+GGG 0.437238 8_1:+42750173 Msa1195020:CDS
GGACTAAGGCGGCCGCAGTT+TGG 0.439857 8_1:+42750271 Msa1195020:CDS
GATTAATGGCGGAGGTGGAT+TGG 0.440550 8_1:+42750249 Msa1195020:CDS
GCGGAGGTGGATTGGGACTA+AGG 0.442001 8_1:+42750257 Msa1195020:CDS
CAGTTTGGTGAGTTTGGACC+CGG 0.446797 8_1:+42750307 Msa1195020:CDS
GATTAATGTTGAGTTGAAAC+CGG 0.448780 8_1:+42750097 Msa1195020:five_prime_UTR
GGAAAACGGACTAAAGCATC+AGG 0.450766 8_1:+42751205 Msa1195020:CDS
CTTGTAGCTTGTGAAACAAA+TGG 0.451137 8_1:-42752811 None:intergenic
GGTTTAAACCGGGATGGTAA+TGG 0.452515 8_1:+42750328 Msa1195020:CDS
TACGACGCCGTTTAGTTTAG+CGG 0.455033 8_1:+42750204 Msa1195020:CDS
TGAAAGGATGAAAATTCTAC+AGG 0.461627 8_1:+42751733 Msa1195020:CDS
GGTTCGGTGCGTGATCTCCT+TGG 0.468651 8_1:-42749949 None:intergenic
GTCGTATTTCCATTCCCATT+CGG 0.470342 8_1:-42750187 None:intergenic
CATCCATGTTCGAGCTAGAA+GGG 0.474849 8_1:+42751336 Msa1195020:CDS
TATTGAACCGAAATGGATCC+AGG 0.476074 8_1:+42750139 Msa1195020:exon
ACCAGAATGGTTACATATGC+AGG 0.483200 8_1:+42752855 Msa1195020:CDS
GGTTCGTTTCCGAATGGGAA+TGG 0.483957 8_1:+42750178 Msa1195020:CDS
GGTAACGGTTTAGGACAGTT+TGG 0.484684 8_1:+42750292 Msa1195020:CDS
TATAATATAGGAGCATTTGA+AGG 0.491142 8_1:-42752968 None:intergenic
AACCATGGTGGTGTTGGTGG+TGG 0.493213 8_1:+42750373 Msa1195020:CDS
ACATGGATGTAATCTTGCTT+AGG 0.502301 8_1:-42751322 None:intergenic
AACAAGAATGAGGGTGGTGA+TGG 0.502949 8_1:+42751229 Msa1195020:CDS
GCGGCGATTAATGGCGGAGG+TGG 0.504274 8_1:+42750244 Msa1195020:CDS
TTGAACCATGGTGGTGTTGG+TGG 0.507286 8_1:+42750370 Msa1195020:CDS
GCAGAGGTTCTTCACCAGAA+TGG 0.515410 8_1:+42752842 Msa1195020:CDS
TCGTGTTTGTTTGTGTAGCA+AGG 0.516464 8_1:+42753276 Msa1195020:three_prime_UTR
ATGTTGAGTTGAAACCGGTT+CGG 0.517549 8_1:+42750102 Msa1195020:five_prime_UTR
TGGATGTAATCTTGCTTAGG+AGG 0.520256 8_1:-42751319 None:intergenic
TGGAGGAAAGGCTTCAATGC+CGG 0.520377 8_1:-42752194 None:intergenic
GGACAATAAACAGGCTAGAA+GGG 0.523957 8_1:+42751700 Msa1195020:intron
GAGAGAAAGAGTGTTAGGTT+CGG 0.525245 8_1:-42749965 None:intergenic
ACTCCTGTCCATTACCATCC+CGG 0.526630 8_1:-42750336 None:intergenic
TTAGGTTATTGGGAAAGCAT+TGG 0.529281 8_1:+42751987 Msa1195020:intron
GTTTGGACCCGGTTTAAACC+GGG 0.537559 8_1:+42750318 Msa1195020:CDS
TCTGGTGAAGAACCTCTGCT+TGG 0.539168 8_1:-42752838 None:intergenic
AAGTGTGTTTCCACCAGTAA+TGG 0.540169 8_1:+42750430 Msa1195020:CDS
CCTCCGCCATTAATCGCCGC+TGG 0.541618 8_1:-42750241 None:intergenic
AGGCGGCCGCAGTTTGGTAA+CGG 0.544003 8_1:+42750277 Msa1195020:CDS
AACGAGGGTTCGTTTCCGAA+TGG 0.545832 8_1:+42750172 Msa1195020:CDS
AAACCGGGATGGTAATGGAC+AGG 0.553598 8_1:+42750333 Msa1195020:CDS
TTACCATCCCGGTTTAAACC+GGG 0.553757 8_1:-42750325 None:intergenic
GCACCATTCTCCACTGTCCA+AGG 0.555247 8_1:+42749932 Msa1195020:five_prime_UTR
GGGAATGAAGAAGTGTCCAT+TGG 0.557216 8_1:-42753023 None:intergenic
TTACATATGCAGGTAGGTGG+TGG 0.558363 8_1:+42752865 Msa1195020:CDS
CTTAAGGTGTTAAAGTCCAA+TGG 0.560546 8_1:+42753007 Msa1195020:three_prime_UTR
AGCATCAGGGAACAAGAATG+AGG 0.561192 8_1:+42751219 Msa1195020:CDS
AGAATGATAGTGATGGAAAA+CGG 0.563521 8_1:+42751191 Msa1195020:intron
TTGTGAAACAAATGGCATGC+CGG 0.566973 8_1:-42752803 None:intergenic
TGCGTGATCTCCTTGGACAG+TGG 0.568345 8_1:-42749942 None:intergenic
CCAGCGGCGATTAATGGCGG+AGG 0.571309 8_1:+42750241 Msa1195020:CDS
GACCCCTTCTAGCTCGAACA+TGG 0.574237 8_1:-42751339 None:intergenic
GAAGGGAAAAGATTAGTGAA+AGG 0.577890 8_1:+42751717 Msa1195020:CDS
ACACTTAGGAGAATCATCTT+CGG 0.578252 8_1:-42750413 None:intergenic
GGTGGTGATGGTAAAGTTGA+AGG 0.578873 8_1:+42751241 Msa1195020:CDS
TTAGGAGGGTCAGAAGGAGG+TGG 0.581023 8_1:-42751304 None:intergenic
TCTCCTTGGACAGTGGAGAA+TGG 0.582146 8_1:-42749935 None:intergenic
GTTAATTCAAGACTCAATAC+CGG 0.586424 8_1:+42752175 Msa1195020:CDS
GTGTTGGTGGTGGTAAGAAG+AGG 0.596088 8_1:+42750383 Msa1195020:CDS
ATCACTTCAGCGCCAAGTGG+AGG 0.597164 8_1:+42752038 Msa1195020:CDS
TCTTGCTTAGGAGGGTCAGA+AGG 0.603501 8_1:-42751310 None:intergenic
AACATTACCTTATTACAACC+AGG 0.621430 8_1:-42751764 None:intergenic
GCATCAGGGAACAAGAATGA+GGG 0.629320 8_1:+42751220 Msa1195020:CDS
TTTCCAGCGGCGATTAATGG+CGG 0.629944 8_1:+42750238 Msa1195020:CDS
GGATGTAATCTTGCTTAGGA+GGG 0.631066 8_1:-42751318 None:intergenic
TCCAGGAGCGATGATGAACG+AGG 0.631852 8_1:+42750156 Msa1195020:CDS
TAATGGCGGTGCCAATGCCG+TGG 0.636011 8_1:+42750447 Msa1195020:CDS
TGCTTAGGAGGGTCAGAAGG+AGG 0.637170 8_1:-42751307 None:intergenic
ATCCATGTTCGAGCTAGAAG+GGG 0.638122 8_1:+42751337 Msa1195020:CDS
GGACCCGGTTTAAACCGGGA+TGG 0.638557 8_1:+42750322 Msa1195020:CDS
GCTACAAGAGAGCCAAGCAG+AGG 0.640109 8_1:+42752826 Msa1195020:CDS
GAGGTGGATTGGGACTAAGG+CGG 0.650298 8_1:+42750260 Msa1195020:CDS
TTACTGGTGGAAACACACTT+AGG 0.651146 8_1:-42750427 None:intergenic
ATTGGCACCGCCATTACTGG+TGG 0.652469 8_1:-42750440 None:intergenic
TGGTTACATATGCAGGTAGG+TGG 0.652770 8_1:+42752862 Msa1195020:CDS
GGAGATCTGGCAGTTTCCAG+CGG 0.658016 8_1:+42750225 Msa1195020:CDS
TCAATCACTTCAGCGCCAAG+TGG 0.659625 8_1:+42752035 Msa1195020:CDS
ATAGAATAGATACCTCCACT+TGG 0.671733 8_1:-42752050 None:intergenic
TACCACCACCAACACCACCA+TGG 0.672358 8_1:-42750375 None:intergenic
TGTGTTTCCACCAGTAATGG+CGG 0.675613 8_1:+42750433 Msa1195020:CDS
TCAGGGAACAAGAATGAGGG+TGG 0.675624 8_1:+42751223 Msa1195020:CDS
AGTCAGTTCAGGAAAGCATG+CGG 0.677155 8_1:+42751267 Msa1195020:CDS
GAATGGTTACATATGCAGGT+AGG 0.689550 8_1:+42752859 Msa1195020:CDS
CCAGGAGCGATGATGAACGA+GGG 0.693487 8_1:+42750157 Msa1195020:CDS
TTACGCGTTCTGCAAGACTG+TGG 0.695452 8_1:-42751375 None:intergenic
GAAAACGGACTAAAGCATCA+GGG 0.713041 8_1:+42751206 Msa1195020:CDS
ATTGTGCAGAATGATAGTGA+TGG 0.726537 8_1:+42751184 Msa1195020:intron
CCTAAACCGTTACCAAACTG+CGG 0.757675 8_1:-42750283 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTTTTTTTAGTTATATT+TGG + chr8_1:42750495-42750514 Msa1195020:intron 10.0%
!!! TATTAAATATATGTATTTTG+GGG + chr8_1:42750566-42750585 Msa1195020:intron 10.0%
!! TTTAATGAATATACTTAAAA+CGG + chr8_1:42750627-42750646 Msa1195020:intron 10.0%
!!! TGTGTTTTTTATTTTATTAT+TGG + chr8_1:42750704-42750723 Msa1195020:intron 10.0%
!! TAACTAAATTATTACAATAA+AGG - chr8_1:42751099-42751118 None:intergenic 10.0%
!! TTAATGGAATTATTATTATT+TGG + chr8_1:42751161-42751180 Msa1195020:intron 10.0%
!!! TCATTTTTGTTTTATAATAT+AGG - chr8_1:42752983-42753002 None:intergenic 10.0%
!!! TTTTTTTAGTTATATTTGGA+AGG + chr8_1:42750499-42750518 Msa1195020:intron 15.0%
!!! GTTTTTAATCATTGTATTTT+TGG + chr8_1:42750660-42750679 Msa1195020:intron 15.0%
!!! TTCACTTTTGATTTTAAAAT+TGG + chr8_1:42750877-42750896 Msa1195020:intron 15.0%
!!! TTAAGGAAAATGTAAATTTT+TGG + chr8_1:42751628-42751647 Msa1195020:intron 15.0%
!! ATAATAATAAACCAAATGAA+AGG + chr8_1:42751851-42751870 Msa1195020:intron 15.0%
!! TAGAAAATATGAGATTATAA+CGG - chr8_1:42751938-42751957 None:intergenic 15.0%
!! AGTTTCAATATAAAAATCAA+GGG - chr8_1:42752241-42752260 None:intergenic 15.0%
!! AAACAAAAATGATATACTTA+AGG + chr8_1:42752991-42753010 Msa1195020:three_prime_UTR 15.0%
!! AGAAAAAAAAATGAATACCA+CGG - chr8_1:42750467-42750486 None:intergenic 20.0%
!!! AGATTTTGCTTCATTTTTTT+TGG + chr8_1:42750752-42750771 Msa1195020:intron 20.0%
!!! GATTTTGCTTCATTTTTTTT+GGG + chr8_1:42750753-42750772 Msa1195020:intron 20.0%
!!! ATTTTTTTTGGGTACTTAAA+AGG + chr8_1:42750764-42750783 Msa1195020:intron 20.0%
!!! AATTTTTCTCCAGAATAATT+AGG - chr8_1:42751076-42751095 None:intergenic 20.0%
!! ATATGTGTATTTGATGTATT+TGG + chr8_1:42751122-42751141 Msa1195020:intron 20.0%
!!! ACTCTTATGTTATTGTTTTT+AGG + chr8_1:42751427-42751446 Msa1195020:intron 20.0%
!!! ATGATGTTAAATCTTGTTTA+AGG + chr8_1:42751611-42751630 Msa1195020:intron 20.0%
!!! AACTGTTATTTTCTTTTCAT+GGG - chr8_1:42751912-42751931 None:intergenic 20.0%
!!! TTTCTTCAATTTAGGTTATT+GGG + chr8_1:42751977-42751996 Msa1195020:intron 20.0%
!!! ATCTATTCTATTTCTGATTT+TGG + chr8_1:42752062-42752081 Msa1195020:intron 20.0%
!! GAGTTTCAATATAAAAATCA+AGG - chr8_1:42752242-42752261 None:intergenic 20.0%
!!! GGAAAATTTTCAAATAAAGA+GGG - chr8_1:42752290-42752309 None:intergenic 20.0%
!!! AGGAAAATTTTCAAATAAAG+AGG - chr8_1:42752291-42752310 None:intergenic 20.0%
!! TATGCAAACTTATTCAAGTT+TGG + chr8_1:42750900-42750919 Msa1195020:intron 25.0%
!! CAATATAAGGACCAAAATTT+TGG + chr8_1:42750955-42750974 Msa1195020:intron 25.0%
! TCATAAAACGGTAGATAATT+GGG - chr8_1:42751001-42751020 None:intergenic 25.0%
! TTGCAATCATTGTCATAAAA+CGG - chr8_1:42751013-42751032 None:intergenic 25.0%
!! TTGATGTATTTGGAATTAGT+CGG + chr8_1:42751132-42751151 Msa1195020:intron 25.0%
! CTAAAAGAGTTGTAAGTAAA+GGG - chr8_1:42751405-42751424 None:intergenic 25.0%
! TCTAAAAGAGTTGTAAGTAA+AGG - chr8_1:42751406-42751425 None:intergenic 25.0%
!!! GTAAATTTTTGGTTTTGTTG+CGG + chr8_1:42751639-42751658 Msa1195020:intron 25.0%
! TCTTGTAATTTCCTTTCATT+TGG - chr8_1:42751865-42751884 None:intergenic 25.0%
!!! CAACTGTTATTTTCTTTTCA+TGG - chr8_1:42751913-42751932 None:intergenic 25.0%
! CATGAAAAGAAAATAACAGT+TGG + chr8_1:42751911-42751930 Msa1195020:intron 25.0%
! ATTGAGACTTTCTTCAATTT+AGG + chr8_1:42751969-42751988 Msa1195020:intron 25.0%
!! CTTTCTTCAATTTAGGTTAT+TGG + chr8_1:42751976-42751995 Msa1195020:intron 25.0%
! GATAAATATACTTGATTGAG+AGG + chr8_1:42752638-42752657 Msa1195020:intron 25.0%
! GAGGATACAGATTAAAAATT+TGG + chr8_1:42752657-42752676 Msa1195020:intron 25.0%
! CATCATTGTTTAAAAAACGT+TGG + chr8_1:42752721-42752740 Msa1195020:intron 25.0%
!! CATTTTATTATTACACGATG+AGG - chr8_1:42752922-42752941 None:intergenic 25.0%
!!! TATAATATAGGAGCATTTGA+AGG - chr8_1:42752971-42752990 None:intergenic 25.0%
!!! CTCTCTCTAGTTTTTTCTTT+CGG + chr8_1:42750007-42750026 Msa1195020:five_prime_UTR 30.0%
!! GATTAATGTTGAGTTGAAAC+CGG + chr8_1:42750097-42750116 Msa1195020:five_prime_UTR 30.0%
TTGAAGTTATTGAACCGAAA+TGG + chr8_1:42750132-42750151 Msa1195020:exon 30.0%
!! TATTTTGGGGAGTGATTAAT+TGG + chr8_1:42750579-42750598 Msa1195020:intron 30.0%
AAATGAAGCAAAATCTTGAG+CGG - chr8_1:42750748-42750767 None:intergenic 30.0%
! TTCACAAACTATTTTGCCTA+TGG + chr8_1:42750828-42750847 Msa1195020:intron 30.0%
GATGTCTTTGCTACAATATA+AGG + chr8_1:42750942-42750961 Msa1195020:intron 30.0%
GTCATAAAACGGTAGATAAT+TGG - chr8_1:42751002-42751021 None:intergenic 30.0%
AATGGTTGTCCTAATTATTC+TGG + chr8_1:42751064-42751083 Msa1195020:intron 30.0%
AATTAGTCGGTGTGATTTAA+TGG + chr8_1:42751145-42751164 Msa1195020:intron 30.0%
! AGAATGATAGTGATGGAAAA+CGG + chr8_1:42751191-42751210 Msa1195020:intron 30.0%
ACTTCTCAATATCAAGAGTT+AGG + chr8_1:42751574-42751593 Msa1195020:intron 30.0%
AACATTCCAATTAGAAACAG+AGG - chr8_1:42751677-42751696 None:intergenic 30.0%
GTTTCTAATTGGAATGTTGA+TGG + chr8_1:42751679-42751698 Msa1195020:intron 30.0%
! AATGTTGATGGACAATAAAC+AGG + chr8_1:42751691-42751710 Msa1195020:intron 30.0%
TGAAAGGATGAAAATTCTAC+AGG + chr8_1:42751733-42751752 Msa1195020:CDS 30.0%
! TTTAGTTCCTGGTTGTAATA+AGG + chr8_1:42751757-42751776 Msa1195020:CDS 30.0%
AACATTACCTTATTACAACC+AGG - chr8_1:42751767-42751786 None:intergenic 30.0%
TGAGAGCAGAAAAAATTAAC+AGG - chr8_1:42751797-42751816 None:intergenic 30.0%
TGCTCTCATATTCACATAAA+CGG + chr8_1:42751809-42751828 Msa1195020:intron 30.0%
GAAAGGAAATTACAAGAAGT+AGG + chr8_1:42751868-42751887 Msa1195020:intron 30.0%
GTTAATTCAAGACTCAATAC+CGG + chr8_1:42752175-42752194 Msa1195020:CDS 30.0%
! TTAACACTTACATCTTTTGG+AGG - chr8_1:42752214-42752233 None:intergenic 30.0%
! TGCTTAACACTTACATCTTT+TGG - chr8_1:42752217-42752236 None:intergenic 30.0%
TGTTGCAAGTATGTATAACA+AGG + chr8_1:42752366-42752385 Msa1195020:intron 30.0%
TGAATAATGTCTGCAAAAGA+TGG - chr8_1:42752397-42752416 None:intergenic 30.0%
TGAACATTGGATTAATCCAA+GGG - chr8_1:42752602-42752621 None:intergenic 30.0%
TTGAACATTGGATTAATCCA+AGG - chr8_1:42752603-42752622 None:intergenic 30.0%
TACAGCTAAAGATTGAACAT+TGG - chr8_1:42752615-42752634 None:intergenic 30.0%
TGGAAAGAGAGAAAAAAACT+AGG - chr8_1:42752757-42752776 None:intergenic 30.0%
!!! TTTTTTCTCTCTTTCCAGTA+TGG + chr8_1:42752760-42752779 Msa1195020:intron 30.0%
CATACCTTTGTGTAGAAAAT+GGG - chr8_1:42753046-42753065 None:intergenic 30.0%
CTCAATATTGTGTACTATAG+TGG + chr8_1:42753131-42753150 Msa1195020:three_prime_UTR 30.0%
!!! CTTTCAGGCTCTTTTTTTTT+TGG + chr8_1:42753155-42753174 Msa1195020:three_prime_UTR 30.0%
!!! TCAAGAGTTGTTTTGAACCA+TGG + chr8_1:42750358-42750377 Msa1195020:CDS 35.0%
ACACTTAGGAGAATCATCTT+CGG - chr8_1:42750416-42750435 None:intergenic 35.0%
AAAAATGAATACCACGGCAT+TGG - chr8_1:42750461-42750480 None:intergenic 35.0%
AATGAAGCAAAATCTTGAGC+GGG - chr8_1:42750747-42750766 None:intergenic 35.0%
! GTTTGAGATTACACTTCCAT+AGG - chr8_1:42750847-42750866 None:intergenic 35.0%
TTCGAACTTCGATGTCTAAA+TGG + chr8_1:42751046-42751065 Msa1195020:intron 35.0%
! ATTGTGCAGAATGATAGTGA+TGG + chr8_1:42751184-42751203 Msa1195020:intron 35.0%
ACATGGATGTAATCTTGCTT+AGG - chr8_1:42751325-42751344 None:intergenic 35.0%
!! TCTATGCCTCTGTTTCTAAT+TGG + chr8_1:42751668-42751687 Msa1195020:intron 35.0%
GAAGGGAAAAGATTAGTGAA+AGG + chr8_1:42751717-42751736 Msa1195020:CDS 35.0%
! ATTCTACAGGATTTAGTTCC+TGG + chr8_1:42751746-42751765 Msa1195020:CDS 35.0%
! TTAGGTTATTGGGAAAGCAT+TGG + chr8_1:42751987-42752006 Msa1195020:intron 35.0%
ATAGAATAGATACCTCCACT+TGG - chr8_1:42752053-42752072 None:intergenic 35.0%
!! ACTTACATCTTTTGGAGGAA+AGG - chr8_1:42752209-42752228 None:intergenic 35.0%
! ATTTTCCTTCTGACAAGACA+TGG + chr8_1:42752303-42752322 Msa1195020:intron 35.0%
ATGAATGATAGCTTCTCACT+AGG + chr8_1:42752498-42752517 Msa1195020:intron 35.0%
TGTTCAATCTTTAGCTGTAG+AGG + chr8_1:42752616-42752635 Msa1195020:intron 35.0%
! CTTGTAGCTTGTGAAACAAA+TGG - chr8_1:42752814-42752833 None:intergenic 35.0%
! CTTAAGGTGTTAAAGTCCAA+TGG + chr8_1:42753007-42753026 Msa1195020:three_prime_UTR 35.0%
! CATTCCCATTTTCTACACAA+AGG + chr8_1:42753039-42753058 Msa1195020:three_prime_UTR 35.0%
CCATACCTTTGTGTAGAAAA+TGG - chr8_1:42753047-42753066 None:intergenic 35.0%
! CCATTTTCTACACAAAGGTA+TGG + chr8_1:42753044-42753063 Msa1195020:three_prime_UTR 35.0%
!!! CAGGCTCTTTTTTTTTTGGT+TGG + chr8_1:42753159-42753178 Msa1195020:three_prime_UTR 35.0%
!! GAGAGAAAGAGTGTTAGGTT+CGG - chr8_1:42749968-42749987 None:intergenic 40.0%
!! AGAGAGAGAGAAAGAGTGTT+AGG - chr8_1:42749973-42749992 None:intergenic 40.0%
!! ATGTTGAGTTGAAACCGGTT+CGG + chr8_1:42750102-42750121 Msa1195020:five_prime_UTR 40.0%
TATTGAACCGAAATGGATCC+AGG + chr8_1:42750139-42750158 Msa1195020:exon 40.0%
GTCGTATTTCCATTCCCATT+CGG - chr8_1:42750190-42750209 None:intergenic 40.0%
CAGATCTCCGCTAAACTAAA+CGG - chr8_1:42750214-42750233 None:intergenic 40.0%
! TTAGGACAGTTTGGTGAGTT+TGG + chr8_1:42750301-42750320 Msa1195020:CDS 40.0%
ATTACCATCCCGGTTTAAAC+CGG - chr8_1:42750329-42750348 None:intergenic 40.0%
!!! AGAGTTGTTTTGAACCATGG+TGG + chr8_1:42750361-42750380 Msa1195020:CDS 40.0%
TTACTGGTGGAAACACACTT+AGG - chr8_1:42750430-42750449 None:intergenic 40.0%
AAGTGTGTTTCCACCAGTAA+TGG + chr8_1:42750430-42750449 Msa1195020:CDS 40.0%
! GAGCGAAGTAGCCAAAATTT+TGG - chr8_1:42750969-42750988 None:intergenic 40.0%
!! GAAAACGGACTAAAGCATCA+GGG + chr8_1:42751206-42751225 Msa1195020:CDS 40.0%
GGATGTAATCTTGCTTAGGA+GGG - chr8_1:42751321-42751340 None:intergenic 40.0%
TGGATGTAATCTTGCTTAGG+AGG - chr8_1:42751322-42751341 None:intergenic 40.0%
TGGACAATAAACAGGCTAGA+AGG + chr8_1:42751699-42751718 Msa1195020:intron 40.0%
GGACAATAAACAGGCTAGAA+GGG + chr8_1:42751700-42751719 Msa1195020:intron 40.0%
! AGTAGGAAAAACGTGCTACT+TGG + chr8_1:42751885-42751904 Msa1195020:intron 40.0%
!! GTAGGAAAAACGTGCTACTT+GGG + chr8_1:42751886-42751905 Msa1195020:intron 40.0%
ACTCACGAGTATAGTAGTTC+TGG + chr8_1:42752334-42752353 Msa1195020:intron 40.0%
! CAAACGTTTGTTGACCATAC+TGG - chr8_1:42752777-42752796 None:intergenic 40.0%
CAACAAACGTTTGATCTTGC+CGG + chr8_1:42752784-42752803 Msa1195020:CDS 40.0%
TTGTGAAACAAATGGCATGC+CGG - chr8_1:42752806-42752825 None:intergenic 40.0%
ACCAGAATGGTTACATATGC+AGG + chr8_1:42752855-42752874 Msa1195020:CDS 40.0%
ACCTGCATATGTAACCATTC+TGG - chr8_1:42752859-42752878 None:intergenic 40.0%
GAATGGTTACATATGCAGGT+AGG + chr8_1:42752859-42752878 Msa1195020:CDS 40.0%
! GGAGCATTTGAAGGATGAAA+TGG - chr8_1:42752962-42752981 None:intergenic 40.0%
! GAGCTGTTACTGTTTTCAAC+AGG + chr8_1:42753066-42753085 Msa1195020:three_prime_UTR 40.0%
!! GTGTACTATAGTGGACTTTC+AGG + chr8_1:42753140-42753159 Msa1195020:three_prime_UTR 40.0%
TCGTGTTTGTTTGTGTAGCA+AGG + chr8_1:42753276-42753295 Msa1195020:three_prime_UTR 40.0%
! TACGACGCCGTTTAGTTTAG+CGG + chr8_1:42750204-42750223 Msa1195020:CDS 45.0%
! CGTTTAGTTTAGCGGAGATC+TGG + chr8_1:42750212-42750231 Msa1195020:CDS 45.0%
! ATTAATGGCGGAGGTGGATT+GGG + chr8_1:42750250-42750269 Msa1195020:CDS 45.0%
CCTAAACCGTTACCAAACTG+CGG - chr8_1:42750286-42750305 None:intergenic 45.0%
! GGTAACGGTTTAGGACAGTT+TGG + chr8_1:42750292-42750311 Msa1195020:CDS 45.0%
AGTTTGGACCCGGTTTAAAC+CGG + chr8_1:42750317-42750336 Msa1195020:CDS 45.0%
TTACCATCCCGGTTTAAACC+GGG - chr8_1:42750328-42750347 None:intergenic 45.0%
GGTTTAAACCGGGATGGTAA+TGG + chr8_1:42750328-42750347 Msa1195020:CDS 45.0%
!! GTTTTGAACCATGGTGGTGT+TGG + chr8_1:42750367-42750386 Msa1195020:CDS 45.0%
TGTGTTTCCACCAGTAATGG+CGG + chr8_1:42750433-42750452 Msa1195020:CDS 45.0%
CAAGTTTGGTCGCGATTTCA+AGG + chr8_1:42750914-42750933 Msa1195020:intron 45.0%
!! GGAAAACGGACTAAAGCATC+AGG + chr8_1:42751205-42751224 Msa1195020:CDS 45.0%
AGCATCAGGGAACAAGAATG+AGG + chr8_1:42751219-42751238 Msa1195020:CDS 45.0%
GCATCAGGGAACAAGAATGA+GGG + chr8_1:42751220-42751239 Msa1195020:CDS 45.0%
AACAAGAATGAGGGTGGTGA+TGG + chr8_1:42751229-42751248 Msa1195020:CDS 45.0%
!! GGTGGTGATGGTAAAGTTGA+AGG + chr8_1:42751241-42751260 Msa1195020:CDS 45.0%
GTTGAAGGAGAAGTCAGTTC+AGG + chr8_1:42751256-42751275 Msa1195020:CDS 45.0%
! AGTCAGTTCAGGAAAGCATG+CGG + chr8_1:42751267-42751286 Msa1195020:CDS 45.0%
ACATCCATGTTCGAGCTAGA+AGG + chr8_1:42751335-42751354 Msa1195020:CDS 45.0%
CATCCATGTTCGAGCTAGAA+GGG + chr8_1:42751336-42751355 Msa1195020:CDS 45.0%
ATCCATGTTCGAGCTAGAAG+GGG + chr8_1:42751337-42751356 Msa1195020:CDS 45.0%
! AGTATTGAACTCTCAGCCCT+TGG + chr8_1:42752583-42752602 Msa1195020:intron 45.0%
TGGTTACATATGCAGGTAGG+TGG + chr8_1:42752862-42752881 Msa1195020:CDS 45.0%
TTACATATGCAGGTAGGTGG+TGG + chr8_1:42752865-42752884 Msa1195020:CDS 45.0%
!! GGGAATGAAGAAGTGTCCAT+TGG - chr8_1:42753026-42753045 None:intergenic 45.0%
CAACAGGCTGCTTCAATTTG+TGG + chr8_1:42753082-42753101 Msa1195020:three_prime_UTR 45.0%
!!! AATATTAAATATATGTATTT+TGG + chr8_1:42750564-42750583 Msa1195020:intron 5.0%
!!! ATATTAAATATATGTATTTT+GGG + chr8_1:42750565-42750584 Msa1195020:intron 5.0%
TCTCCTTGGACAGTGGAGAA+TGG - chr8_1:42749938-42749957 None:intergenic 50.0%
!! GAGTTGAAACCGGTTCGGTT+CGG + chr8_1:42750107-42750126 Msa1195020:five_prime_UTR 50.0%
!! AGTTGAAACCGGTTCGGTTC+GGG + chr8_1:42750108-42750127 Msa1195020:five_prime_UTR 50.0%
ACTTCAAACCCGAACCGAAC+CGG - chr8_1:42750119-42750138 None:intergenic 50.0%
CATCGCTCCTGGATCCATTT+CGG - chr8_1:42750149-42750168 None:intergenic 50.0%
AACGAGGGTTCGTTTCCGAA+TGG + chr8_1:42750172-42750191 Msa1195020:CDS 50.0%
ACGAGGGTTCGTTTCCGAAT+GGG + chr8_1:42750173-42750192 Msa1195020:CDS 50.0%
GGTTCGTTTCCGAATGGGAA+TGG + chr8_1:42750178-42750197 Msa1195020:CDS 50.0%
CAGTTTCCAGCGGCGATTAA+TGG + chr8_1:42750235-42750254 Msa1195020:CDS 50.0%
TTTCCAGCGGCGATTAATGG+CGG + chr8_1:42750238-42750257 Msa1195020:CDS 50.0%
! GATTAATGGCGGAGGTGGAT+TGG + chr8_1:42750249-42750268 Msa1195020:CDS 50.0%
!! CCGCAGTTTGGTAACGGTTT+AGG + chr8_1:42750283-42750302 Msa1195020:CDS 50.0%
! CAGTTTGGTGAGTTTGGACC+CGG + chr8_1:42750307-42750326 Msa1195020:CDS 50.0%
GTTTGGACCCGGTTTAAACC+GGG + chr8_1:42750318-42750337 Msa1195020:CDS 50.0%
!! AAACCGGGATGGTAATGGAC+AGG + chr8_1:42750333-42750352 Msa1195020:CDS 50.0%
ACTCCTGTCCATTACCATCC+CGG - chr8_1:42750339-42750358 None:intergenic 50.0%
! TTGAACCATGGTGGTGTTGG+TGG + chr8_1:42750370-42750389 Msa1195020:CDS 50.0%
!! GTGTTGGTGGTGGTAAGAAG+AGG + chr8_1:42750383-42750402 Msa1195020:CDS 50.0%
TCAGGGAACAAGAATGAGGG+TGG + chr8_1:42751223-42751242 Msa1195020:CDS 50.0%
TCTTGCTTAGGAGGGTCAGA+AGG - chr8_1:42751313-42751332 None:intergenic 50.0%
TTACGCGTTCTGCAAGACTG+TGG - chr8_1:42751378-42751397 None:intergenic 50.0%
!! TGAGTTGAGAAGCGCTGTTG+TGG + chr8_1:42751507-42751526 Msa1195020:intron 50.0%
TCAATCACTTCAGCGCCAAG+TGG + chr8_1:42752035-42752054 Msa1195020:CDS 50.0%
TGGAGGAAAGGCTTCAATGC+CGG - chr8_1:42752197-42752216 None:intergenic 50.0%
GCTAGCCATGTCTTGTCAGA+AGG - chr8_1:42752311-42752330 None:intergenic 50.0%
AAGCTTGCAACCTCCAGTCT+CGG + chr8_1:42752547-42752566 Msa1195020:intron 50.0%
AACCTCCAGTCTCGGCATAA+TGG + chr8_1:42752555-42752574 Msa1195020:intron 50.0%
ACCTCCAGTCTCGGCATAAT+GGG + chr8_1:42752556-42752575 Msa1195020:intron 50.0%
TCTGGTGAAGAACCTCTGCT+TGG - chr8_1:42752841-42752860 None:intergenic 50.0%
GCAGAGGTTCTTCACCAGAA+TGG + chr8_1:42752842-42752861 Msa1195020:CDS 50.0%
GCACCATTCTCCACTGTCCA+AGG + chr8_1:42749932-42749951 Msa1195020:five_prime_UTR 55.0%
TGCGTGATCTCCTTGGACAG+TGG - chr8_1:42749945-42749964 None:intergenic 55.0%
TCCAGGAGCGATGATGAACG+AGG + chr8_1:42750156-42750175 Msa1195020:CDS 55.0%
CCAGGAGCGATGATGAACGA+GGG + chr8_1:42750157-42750176 Msa1195020:CDS 55.0%
GGAGATCTGGCAGTTTCCAG+CGG + chr8_1:42750225-42750244 Msa1195020:CDS 55.0%
!! GAGGTGGATTGGGACTAAGG+CGG + chr8_1:42750260-42750279 Msa1195020:CDS 55.0%
!! AACCATGGTGGTGTTGGTGG+TGG + chr8_1:42750373-42750392 Msa1195020:CDS 55.0%
TACCACCACCAACACCACCA+TGG - chr8_1:42750378-42750397 None:intergenic 55.0%
ATTGGCACCGCCATTACTGG+TGG - chr8_1:42750443-42750462 None:intergenic 55.0%
TTAGGAGGGTCAGAAGGAGG+TGG - chr8_1:42751307-42751326 None:intergenic 55.0%
TGCTTAGGAGGGTCAGAAGG+AGG - chr8_1:42751310-42751329 None:intergenic 55.0%
!! GACCCCTTCTAGCTCGAACA+TGG - chr8_1:42751342-42751361 None:intergenic 55.0%
TTGTGGCTGCGCGACATCAT+AGG + chr8_1:42751524-42751543 Msa1195020:intron 55.0%
ATCACTTCAGCGCCAAGTGG+AGG + chr8_1:42752038-42752057 Msa1195020:CDS 55.0%
! TCCAGTCTCGGCATAATGGG+CGG + chr8_1:42752559-42752578 Msa1195020:intron 55.0%
GCTACAAGAGAGCCAAGCAG+AGG + chr8_1:42752826-42752845 Msa1195020:CDS 55.0%
!! GGTTCGGTGCGTGATCTCCT+TGG - chr8_1:42749952-42749971 None:intergenic 60.0%
CCCTCGTTCATCATCGCTCC+TGG - chr8_1:42750160-42750179 None:intergenic 60.0%
!! GCGGAGGTGGATTGGGACTA+AGG + chr8_1:42750257-42750276 Msa1195020:CDS 60.0%
AGGCGGCCGCAGTTTGGTAA+CGG + chr8_1:42750277-42750296 Msa1195020:CDS 60.0%
GGACCCGGTTTAAACCGGGA+TGG + chr8_1:42750322-42750341 Msa1195020:CDS 60.0%
!! GGCATTGGCACCGCCATTAC+TGG - chr8_1:42750446-42750465 None:intergenic 60.0%
! TAATGGCGGTGCCAATGCCG+TGG + chr8_1:42750447-42750466 Msa1195020:CDS 60.0%
GCCCATTATGCCGAGACTGG+AGG - chr8_1:42752560-42752579 None:intergenic 60.0%
!! CCAGTCTCGGCATAATGGGC+GGG + chr8_1:42752560-42752579 Msa1195020:intron 60.0%
CCTCCGCCATTAATCGCCGC+TGG - chr8_1:42750244-42750263 None:intergenic 65.0%
CCAGCGGCGATTAATGGCGG+AGG + chr8_1:42750241-42750260 Msa1195020:CDS 65.0%
! GCGGCGATTAATGGCGGAGG+TGG + chr8_1:42750244-42750263 Msa1195020:CDS 65.0%
GGACTAAGGCGGCCGCAGTT+TGG + chr8_1:42750271-42750290 Msa1195020:CDS 65.0%
CCCGCCCATTATGCCGAGAC+TGG - chr8_1:42752563-42752582 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 42749880 42753331 42749880 ID=Msa1195020;Name=Msa1195020
chr8_1 mRNA 42749880 42753331 42749880 ID=Msa1195020-mRNA-1;Parent=Msa1195020;Name=Msa1195020-mRNA-1;_AED=0.05;_eAED=0.05;_QI=271|1|1|1|1|1|6|425|284
chr8_1 exon 42749880 42750468 42749880 ID=Msa1195020-mRNA-1:exon:13769;Parent=Msa1195020-mRNA-1
chr8_1 exon 42751193 42751393 42751193 ID=Msa1195020-mRNA-1:exon:13770;Parent=Msa1195020-mRNA-1
chr8_1 exon 42751713 42751778 42751713 ID=Msa1195020-mRNA-1:exon:13771;Parent=Msa1195020-mRNA-1
chr8_1 exon 42751991 42752059 42751991 ID=Msa1195020-mRNA-1:exon:13772;Parent=Msa1195020-mRNA-1
chr8_1 exon 42752151 42752222 42752151 ID=Msa1195020-mRNA-1:exon:13773;Parent=Msa1195020-mRNA-1
chr8_1 exon 42752778 42753331 42752778 ID=Msa1195020-mRNA-1:exon:13774;Parent=Msa1195020-mRNA-1
chr8_1 five_prime_UTR 42749880 42750150 42749880 ID=Msa1195020-mRNA-1:five_prime_utr;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42750151 42750468 42750151 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42751193 42751393 42751193 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42751713 42751778 42751713 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42751991 42752059 42751991 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42752151 42752222 42752151 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 CDS 42752778 42752906 42752778 ID=Msa1195020-mRNA-1:cds;Parent=Msa1195020-mRNA-1
chr8_1 three_prime_UTR 42752907 42753331 42752907 ID=Msa1195020-mRNA-1:three_prime_utr;Parent=Msa1195020-mRNA-1
Gene Sequence

>Msa1195020

ATGGATCCAGGAGCGATGATGAACGAGGGTTCGTTTCCGAATGGGAATGGAAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCGGCGATTAATGGCGGAGGTGGATTGGGACTAAGGCGGCCGCAGTTTGGTAACGGTTTAGGACAGTTTGGTGAGTTTGGACCCGGTTTAAACCGGGATGGTAATGGACAGGAGTCAAGAGTTGTTTTGAACCATGGTGGTGTTGGTGGTGGTAAGAAGAGGCGTGACTCCGAAGATGATTCTCCTAAGTGTGTTTCCACCAGTAATGGCGGTGCCAATGCCGTGAATGATAGTGATGGAAAACGGACTAAAGCATCAGGGAACAAGAATGAGGGTGGTGATGGTAAAGTTGAAGGAGAAGTCAGTTCAGGAAAGCATGCGGAGCAAAGCAGTAAACCACCTCCTTCTGACCCTCCTAAGCAAGATTACATCCATGTTCGAGCTAGAAGGGGTCAAGCTACTGATAGCCACAGTCTTGCAGAACGCGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTACAGGATTTAGTTCCTGGTTGTAATAAGGTTATTGGGAAAGCATTGGTGCTTGATGAGATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGTTTTTATCAATGAAACTTGAAGCAGTTAATTCAAGACTCAATACCGGCATTGAAGCCTTTCCTCCAAAAGATTATGGTCAACAAACGTTTGATCTTGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGTTCTTCACCAGAATGGTTACATATGCAGGTAGGTGGTGGTTTCGAAAGAACGACGTAG
Protein sequence

>Msa1195020

MDPGAMMNEGSFPNGNGNTTPFSLAEIWQFPAAINGGGGLGLRRPQFGNGLGQFGEFGPGLNRDGNGQESRVVLNHGGVGGGKKRRDSEDDSPKCVSTSNGGANAVNDSDGKRTKASGNKNEGGDGKVEGEVSSGKHAEQSSKPPPSDPPKQDYIHVRARRGQATDSHSLAERARREKISERMKILQDLVPGCNKVIGKALVLDEIINYIQSLQRQVEFLSMKLEAVNSRLNTGIEAFPPKDYGQQTFDLAGMPFVSQATREPSRGSSPEWLHMQVGGGFERTT*