AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1196480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1196480 MtrunA17_Chr8g0362131 98.913 92 1 0 1 92 1 92 1.66e-61 181
Msa1196480 MtrunA17_Chr5g0416431 82.051 78 13 1 16 92 16 93 1.64e-39 126
Msa1196480 MtrunA17_Chr7g0217141 76.087 92 20 2 1 91 1 91 4.65e-38 122
Msa1196480 MtrunA17_Chr6g0453041 75.000 76 18 1 17 91 15 90 6.74e-32 107
Msa1196480 MtrunA17_Chr3g0093001 71.429 77 19 2 17 92 17 91 2.98e-30 102
Msa1196480 MtrunA17_Chr7g0237771 50.617 81 34 3 1 80 1 76 4.46e-13 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1196480 AT3G47710.1 69.149 94 24 3 1 92 1 91 3.30e-36 118
Msa1196480 AT3G28857.1 65.957 94 28 2 1 92 1 92 8.19e-36 117
Msa1196480 AT1G26945.1 78.378 74 15 1 19 91 19 92 1.29e-33 111
Msa1196480 AT5G15160.1 68.000 75 22 1 19 91 18 92 1.51e-31 106
Msa1196480 AT1G74500.1 74.026 77 16 2 17 91 17 91 6.80e-31 104
Msa1196480 AT5G39860.1 66.667 78 24 1 17 92 15 92 8.50e-31 104

Find 58 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCGATTGTCACAACTTT+TGG 0.247784 8_1:+45752694 Msa1196480:CDS
GGCTAGGCAAGTTATTAAAT+AGG 0.271481 8_1:-45750841 None:intergenic
TTATCATCAACCTCTCCTTA+TGG 0.309062 8_1:+45752863 Msa1196480:three_prime_UTR
GTATGTCCCTATGGGGTTAA+AGG 0.327120 8_1:-45750750 None:intergenic
TTGAGCACTGTTAGGGTCTA+TGG 0.329793 8_1:-45752720 None:intergenic
CCATAGGGACATACGCAAAA+AGG 0.331547 8_1:+45750759 Msa1196480:five_prime_UTR
AGCTAGTGAGACATATTAAA+AGG 0.335554 8_1:-45752819 None:intergenic
AAAGTGTTTGAGAAAGGTAT+TGG 0.351552 8_1:-45750863 None:intergenic
ATAGCTGCTTGAGCACTGTT+AGG 0.352323 8_1:-45752728 None:intergenic
GGGAGACAAGTGAATGAAAT+AGG 0.380265 8_1:-45750806 None:intergenic
TATCATCAACCTCTCCTTAT+GGG 0.387409 8_1:+45752864 Msa1196480:three_prime_UTR
TAGTGTTAATTAGTGTGAAA+TGG 0.399544 8_1:+45752924 Msa1196480:three_prime_UTR
GCTAGGCAAGTTATTAAATA+GGG 0.401242 8_1:-45750840 None:intergenic
TGGGAACGCCTTGAGAGTTC+AGG 0.406573 8_1:-45751356 None:intergenic
ACAACCTAATCTCTCTTTCA+TGG 0.408857 8_1:+45750927 Msa1196480:five_prime_UTR
AGAAAATTATTATACCTTAT+GGG 0.418461 8_1:-45751375 None:intergenic
GATGGACGAGAGAGAGAAAA+GGG 0.419581 8_1:-45750953 None:intergenic
GTGCTCAAGCAGCTATCATT+AGG 0.423047 8_1:+45752735 Msa1196480:CDS
AAAGAGGTCCCCATGCAGTT+GGG 0.423561 8_1:+45750698 Msa1196480:five_prime_UTR
CATGGGGACCTCTTTGTGTC+AGG 0.427728 8_1:-45750690 None:intergenic
TCTTCTGCTAGACATTGTTA+AGG 0.430216 8_1:-45751249 None:intergenic
AAGTGTTTGAGAAAGGTATT+GGG 0.430347 8_1:-45750862 None:intergenic
TTTGTAGGTTTCAGCTGCTA+AGG 0.463834 8_1:+45752613 Msa1196480:intron
ACACGAAAACGTTACCCATA+AGG 0.478677 8_1:-45752878 None:intergenic
AGATCTCGTTCAAGACAATC+TGG 0.480776 8_1:+45751269 Msa1196480:CDS
AGGATAAAAGTGTTTGAGAA+AGG 0.482084 8_1:-45750869 None:intergenic
AGGTACAAGTTGTTGTAACT+TGG 0.482816 8_1:-45751336 None:intergenic
ACTCTCAAGGCGTTCCCATA+AGG 0.483554 8_1:+45751361 Msa1196480:CDS
AAGAGGTCCCCATGCAGTTG+GGG 0.496582 8_1:+45750699 Msa1196480:five_prime_UTR
TTTGAGAAAGGTATTGGGCT+AGG 0.496830 8_1:-45750857 None:intergenic
TGATGGACGAGAGAGAGAAA+AGG 0.497265 8_1:-45750954 None:intergenic
ACAAGTGAATGAAATAGGAA+TGG 0.501907 8_1:-45750801 None:intergenic
TAGAGTCTAGGTAGCTACTT+AGG 0.512562 8_1:-45751081 None:intergenic
TTCTCAGCTGAGACAAGTGA+TGG 0.516764 8_1:-45750971 None:intergenic
CAAGTGAATGAAATAGGAAT+GGG 0.519289 8_1:-45750800 None:intergenic
TAGCTGCTTGAGCACTGTTA+GGG 0.522205 8_1:-45752727 None:intergenic
TTGAAGGAAACCTAGAGTCT+AGG 0.523348 8_1:-45751093 None:intergenic
CAAAGAGGTCCCCATGCAGT+TGG 0.525978 8_1:+45750697 Msa1196480:five_prime_UTR
ACATTTCCTTTAACCCCATA+GGG 0.527841 8_1:+45750744 Msa1196480:five_prime_UTR
GTAGCCATGAAAGAGAGATT+AGG 0.531428 8_1:-45750931 None:intergenic
GAGGTCCCCATGCAGTTGGG+GGG 0.536452 8_1:+45750701 Msa1196480:five_prime_UTR
TAAGTAGCTACCTAGACTCT+AGG 0.537832 8_1:+45751083 Msa1196480:five_prime_UTR
GAGAATGTGTCAGTTGGTGT+TGG 0.544938 8_1:-45750901 None:intergenic
TGTCCAAAAGTTGTGACAAT+CGG 0.545364 8_1:-45752697 None:intergenic
AGAGGTCCCCATGCAGTTGG+GGG 0.547715 8_1:+45750700 Msa1196480:five_prime_UTR
CACATTTCCTTTAACCCCAT+AGG 0.548119 8_1:+45750743 Msa1196480:five_prime_UTR
TCAGTGCATGAGACTTCCAC+TGG 0.554389 8_1:-45752963 None:intergenic
GTTGGTGTTGGAAGTTGAAG+AGG 0.564686 8_1:-45750889 None:intergenic
AGGTCCCCATGCAGTTGGGG+GGG 0.573436 8_1:+45750702 Msa1196480:five_prime_UTR
TTAAATAGGGATATTACGTG+AGG 0.596488 8_1:-45750827 None:intergenic
AACTTGTACCTGAACTCTCA+AGG 0.607839 8_1:+45751348 Msa1196480:CDS
ATGCTGAGCCTGACACAAAG+AGG 0.615742 8_1:+45750682 None:intergenic
TGTTGAGAGAATGTGTCAGT+TGG 0.622998 8_1:-45750907 None:intergenic
GAGACATATTAAAAGGCATG+TGG 0.632495 8_1:-45752812 None:intergenic
TAAATAGGGATATTACGTGA+GGG 0.635260 8_1:-45750826 None:intergenic
AGATCACAAACTTCATCCAG+TGG 0.666441 8_1:+45752947 Msa1196480:three_prime_UTR
AAAACGTTACCCATAAGGAG+AGG 0.710641 8_1:-45752873 None:intergenic
TTGAGCATCAGAGATCTCAG+TGG 0.738554 8_1:-45751297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GCTTTTTTTTTTTTTTTTGA+AGG - chr8_1:45751112-45751131 None:intergenic 15.0%
!! AGAAAATTATTATACCTTAT+GGG - chr8_1:45751378-45751397 None:intergenic 15.0%
!! AAGAAAATTATTATACCTTA+TGG - chr8_1:45751379-45751398 None:intergenic 15.0%
!!! AAATCAATTTTATGTGTTTA+GGG - chr8_1:45751479-45751498 None:intergenic 15.0%
!!! AAATTGATTTCTAAATGATA+TGG + chr8_1:45751489-45751508 Msa1196480:intron 15.0%
!!! TTTTGAATGAAATAAATTTG+AGG - chr8_1:45751689-45751708 None:intergenic 15.0%
!! TTTATTTCATTCAAAATCAA+TGG + chr8_1:45751693-45751712 Msa1196480:intron 15.0%
!!! AAATGATCTTTAATTTTTTC+TGG + chr8_1:45751810-45751829 Msa1196480:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_1:45751932-45751951 Msa1196480:intron 15.0%
!!! AAATGATCTTTAATTTTTTC+TGG + chr8_1:45751810-45751829 Msa1196480:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_1:45751932-45751951 Msa1196480:intron 15.0%
!!! TGTTTATTGTATTATTTTGT+AGG + chr8_1:45752598-45752617 Msa1196480:intron 15.0%
!!! GCTTTTTTTTTTTTTTTTGA+AGG - chr8_1:45751112-45751131 None:intergenic 15.0%
!! AGAAAATTATTATACCTTAT+GGG - chr8_1:45751378-45751397 None:intergenic 15.0%
!! AAGAAAATTATTATACCTTA+TGG - chr8_1:45751379-45751398 None:intergenic 15.0%
!!! AAATCAATTTTATGTGTTTA+GGG - chr8_1:45751479-45751498 None:intergenic 15.0%
!!! AAATTGATTTCTAAATGATA+TGG + chr8_1:45751489-45751508 Msa1196480:intron 15.0%
!!! TTTTGAATGAAATAAATTTG+AGG - chr8_1:45751689-45751708 None:intergenic 15.0%
!! TTTATTTCATTCAAAATCAA+TGG + chr8_1:45751693-45751712 Msa1196480:intron 15.0%
!!! AAATGATCTTTAATTTTTTC+TGG + chr8_1:45751810-45751829 Msa1196480:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_1:45751932-45751951 Msa1196480:intron 15.0%
!!! AAATGATCTTTAATTTTTTC+TGG + chr8_1:45751810-45751829 Msa1196480:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_1:45751932-45751951 Msa1196480:intron 15.0%
!!! TGTTTATTGTATTATTTTGT+AGG + chr8_1:45752598-45752617 Msa1196480:intron 15.0%
!!! GAAATCAATTTTATGTGTTT+AGG - chr8_1:45751480-45751499 None:intergenic 20.0%
!!! GAAATCAATTTTATGTGTTT+AGG - chr8_1:45751480-45751499 None:intergenic 20.0%
! TAAAAGAGAGAATACAGAAA+AGG - chr8_1:45751403-45751422 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_1:45751838-45751857 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_1:45751838-45751857 None:intergenic 25.0%
!!! AAATTTGTTGTGTTCAAGAA+AGG + chr8_1:45752297-45752316 Msa1196480:intron 25.0%
!!! AATTTGTTGTGTTCAAGAAA+GGG + chr8_1:45752298-45752317 Msa1196480:intron 25.0%
! TAGTGTTAATTAGTGTGAAA+TGG + chr8_1:45752924-45752943 Msa1196480:three_prime_UTR 25.0%
! TAAAAGAGAGAATACAGAAA+AGG - chr8_1:45751403-45751422 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_1:45751838-45751857 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_1:45751838-45751857 None:intergenic 25.0%
!!! AAATTTGTTGTGTTCAAGAA+AGG + chr8_1:45752297-45752316 Msa1196480:intron 25.0%
!!! AATTTGTTGTGTTCAAGAAA+GGG + chr8_1:45752298-45752317 Msa1196480:intron 25.0%
! TAGTGTTAATTAGTGTGAAA+TGG + chr8_1:45752924-45752943 Msa1196480:three_prime_UTR 25.0%
CAAGTGAATGAAATAGGAAT+GGG - chr8_1:45750803-45750822 None:intergenic 30.0%
ACAAGTGAATGAAATAGGAA+TGG - chr8_1:45750804-45750823 None:intergenic 30.0%
TAAATAGGGATATTACGTGA+GGG - chr8_1:45750829-45750848 None:intergenic 30.0%
TTAAATAGGGATATTACGTG+AGG - chr8_1:45750830-45750849 None:intergenic 30.0%
GCTAGGCAAGTTATTAAATA+GGG - chr8_1:45750843-45750862 None:intergenic 30.0%
!! AAGTGTTTGAGAAAGGTATT+GGG - chr8_1:45750865-45750884 None:intergenic 30.0%
! AAAGTGTTTGAGAAAGGTAT+TGG - chr8_1:45750866-45750885 None:intergenic 30.0%
!! AGGATAAAAGTGTTTGAGAA+AGG - chr8_1:45750872-45750891 None:intergenic 30.0%
!!! ATTGTTAAGGTTTTTGAGGT+AGG - chr8_1:45751239-45751258 None:intergenic 30.0%
! AGACATTGTTAAGGTTTTTG+AGG - chr8_1:45751243-45751262 None:intergenic 30.0%
GGATGAAAATGACAATAGTT+TGG - chr8_1:45751770-45751789 None:intergenic 30.0%
GTCAACAAAAAACTTTAGCT+TGG - chr8_1:45752250-45752269 None:intergenic 30.0%
! CTACACACATATTTTATGTG+TGG + chr8_1:45752378-45752397 Msa1196480:intron 30.0%
! ACATATTTTATGTGTGGCTT+TGG + chr8_1:45752384-45752403 Msa1196480:intron 30.0%
ATGATATATGCATTGCAAGT+GGG - chr8_1:45752501-45752520 None:intergenic 30.0%
AATGATATATGCATTGCAAG+TGG - chr8_1:45752502-45752521 None:intergenic 30.0%
! CAACAAATTTTCAGTTGCTA+TGG - chr8_1:45752539-45752558 None:intergenic 30.0%
AGCTAGTGAGACATATTAAA+AGG - chr8_1:45752822-45752841 None:intergenic 30.0%
CAAGTGAATGAAATAGGAAT+GGG - chr8_1:45750803-45750822 None:intergenic 30.0%
ACAAGTGAATGAAATAGGAA+TGG - chr8_1:45750804-45750823 None:intergenic 30.0%
TAAATAGGGATATTACGTGA+GGG - chr8_1:45750829-45750848 None:intergenic 30.0%
TTAAATAGGGATATTACGTG+AGG - chr8_1:45750830-45750849 None:intergenic 30.0%
GCTAGGCAAGTTATTAAATA+GGG - chr8_1:45750843-45750862 None:intergenic 30.0%
!! AAGTGTTTGAGAAAGGTATT+GGG - chr8_1:45750865-45750884 None:intergenic 30.0%
! AAAGTGTTTGAGAAAGGTAT+TGG - chr8_1:45750866-45750885 None:intergenic 30.0%
!! AGGATAAAAGTGTTTGAGAA+AGG - chr8_1:45750872-45750891 None:intergenic 30.0%
!!! ATTGTTAAGGTTTTTGAGGT+AGG - chr8_1:45751239-45751258 None:intergenic 30.0%
! AGACATTGTTAAGGTTTTTG+AGG - chr8_1:45751243-45751262 None:intergenic 30.0%
GGATGAAAATGACAATAGTT+TGG - chr8_1:45751770-45751789 None:intergenic 30.0%
GTCAACAAAAAACTTTAGCT+TGG - chr8_1:45752250-45752269 None:intergenic 30.0%
! CTACACACATATTTTATGTG+TGG + chr8_1:45752378-45752397 Msa1196480:intron 30.0%
! ACATATTTTATGTGTGGCTT+TGG + chr8_1:45752384-45752403 Msa1196480:intron 30.0%
ATGATATATGCATTGCAAGT+GGG - chr8_1:45752501-45752520 None:intergenic 30.0%
AATGATATATGCATTGCAAG+TGG - chr8_1:45752502-45752521 None:intergenic 30.0%
! CAACAAATTTTCAGTTGCTA+TGG - chr8_1:45752539-45752558 None:intergenic 30.0%
AGCTAGTGAGACATATTAAA+AGG - chr8_1:45752822-45752841 None:intergenic 30.0%
ACATTTCCTTTAACCCCATA+GGG + chr8_1:45750744-45750763 Msa1196480:five_prime_UTR 35.0%
GGCTAGGCAAGTTATTAAAT+AGG - chr8_1:45750844-45750863 None:intergenic 35.0%
ACAACCTAATCTCTCTTTCA+TGG + chr8_1:45750927-45750946 Msa1196480:five_prime_UTR 35.0%
!!! AAGGTTTTTGAGGTAGGTAT+AGG - chr8_1:45751233-45751252 None:intergenic 35.0%
TCTTCTGCTAGACATTGTTA+AGG - chr8_1:45751252-45751271 None:intergenic 35.0%
AGGTACAAGTTGTTGTAACT+TGG - chr8_1:45751339-45751358 None:intergenic 35.0%
!!! ATTTTATGTGTGGCTTTGGT+TGG + chr8_1:45752388-45752407 Msa1196480:intron 35.0%
TTTGTGGGTAAACGAGTTAA+GGG - chr8_1:45752451-45752470 None:intergenic 35.0%
CATCAAAAACTTGCATAGAG+AGG + chr8_1:45752658-45752677 Msa1196480:CDS 35.0%
TGTCCAAAAGTTGTGACAAT+CGG - chr8_1:45752700-45752719 None:intergenic 35.0%
! GAGACATATTAAAAGGCATG+TGG - chr8_1:45752815-45752834 None:intergenic 35.0%
TTATCATCAACCTCTCCTTA+TGG + chr8_1:45752863-45752882 Msa1196480:three_prime_UTR 35.0%
TATCATCAACCTCTCCTTAT+GGG + chr8_1:45752864-45752883 Msa1196480:three_prime_UTR 35.0%
ACATTTCCTTTAACCCCATA+GGG + chr8_1:45750744-45750763 Msa1196480:five_prime_UTR 35.0%
GGCTAGGCAAGTTATTAAAT+AGG - chr8_1:45750844-45750863 None:intergenic 35.0%
ACAACCTAATCTCTCTTTCA+TGG + chr8_1:45750927-45750946 Msa1196480:five_prime_UTR 35.0%
!!! AAGGTTTTTGAGGTAGGTAT+AGG - chr8_1:45751233-45751252 None:intergenic 35.0%
TCTTCTGCTAGACATTGTTA+AGG - chr8_1:45751252-45751271 None:intergenic 35.0%
AGGTACAAGTTGTTGTAACT+TGG - chr8_1:45751339-45751358 None:intergenic 35.0%
!!! ATTTTATGTGTGGCTTTGGT+TGG + chr8_1:45752388-45752407 Msa1196480:intron 35.0%
TTTGTGGGTAAACGAGTTAA+GGG - chr8_1:45752451-45752470 None:intergenic 35.0%
CATCAAAAACTTGCATAGAG+AGG + chr8_1:45752658-45752677 Msa1196480:CDS 35.0%
TGTCCAAAAGTTGTGACAAT+CGG - chr8_1:45752700-45752719 None:intergenic 35.0%
! GAGACATATTAAAAGGCATG+TGG - chr8_1:45752815-45752834 None:intergenic 35.0%
TTATCATCAACCTCTCCTTA+TGG + chr8_1:45752863-45752882 Msa1196480:three_prime_UTR 35.0%
TATCATCAACCTCTCCTTAT+GGG + chr8_1:45752864-45752883 Msa1196480:three_prime_UTR 35.0%
CACATTTCCTTTAACCCCAT+AGG + chr8_1:45750743-45750762 Msa1196480:five_prime_UTR 40.0%
! TTTTTGCGTATGTCCCTATG+GGG - chr8_1:45750760-45750779 None:intergenic 40.0%
! CTTTTTGCGTATGTCCCTAT+GGG - chr8_1:45750761-45750780 None:intergenic 40.0%
CATACGCAAAAAGGACAGTA+TGG + chr8_1:45750768-45750787 Msa1196480:five_prime_UTR 40.0%
GGGAGACAAGTGAATGAAAT+AGG - chr8_1:45750809-45750828 None:intergenic 40.0%
!! TTTGAGAAAGGTATTGGGCT+AGG - chr8_1:45750860-45750879 None:intergenic 40.0%
TGTTGAGAGAATGTGTCAGT+TGG - chr8_1:45750910-45750929 None:intergenic 40.0%
GTAGCCATGAAAGAGAGATT+AGG - chr8_1:45750934-45750953 None:intergenic 40.0%
TAGAGTCTAGGTAGCTACTT+AGG - chr8_1:45751084-45751103 None:intergenic 40.0%
TAAGTAGCTACCTAGACTCT+AGG + chr8_1:45751083-45751102 Msa1196480:five_prime_UTR 40.0%
TTGAAGGAAACCTAGAGTCT+AGG - chr8_1:45751096-45751115 None:intergenic 40.0%
AGATCTCGTTCAAGACAATC+TGG + chr8_1:45751269-45751288 Msa1196480:CDS 40.0%
AACTTGTACCTGAACTCTCA+AGG + chr8_1:45751348-45751367 Msa1196480:CDS 40.0%
TAGTGTGTGTTCGTTCTACA+GGG - chr8_1:45751723-45751742 None:intergenic 40.0%
AAACGAGTTAAGGGGATTCA+GGG - chr8_1:45752442-45752461 None:intergenic 40.0%
TAAACGAGTTAAGGGGATTC+AGG - chr8_1:45752443-45752462 None:intergenic 40.0%
TTGTGGGTAAACGAGTTAAG+GGG - chr8_1:45752450-45752469 None:intergenic 40.0%
GTTTGTGGGTAAACGAGTTA+AGG - chr8_1:45752452-45752471 None:intergenic 40.0%
TCGTTTACCCACAAACTTGT+AGG + chr8_1:45752456-45752475 Msa1196480:intron 40.0%
CGTTTACCCACAAACTTGTA+GGG + chr8_1:45752457-45752476 Msa1196480:intron 40.0%
TTTGTAGGTTTCAGCTGCTA+AGG + chr8_1:45752613-45752632 Msa1196480:intron 40.0%
! TGACCGATTGTCACAACTTT+TGG + chr8_1:45752694-45752713 Msa1196480:CDS 40.0%
AAAACGTTACCCATAAGGAG+AGG - chr8_1:45752876-45752895 None:intergenic 40.0%
ACACGAAAACGTTACCCATA+AGG - chr8_1:45752881-45752900 None:intergenic 40.0%
AGATCACAAACTTCATCCAG+TGG + chr8_1:45752947-45752966 Msa1196480:three_prime_UTR 40.0%
CACATTTCCTTTAACCCCAT+AGG + chr8_1:45750743-45750762 Msa1196480:five_prime_UTR 40.0%
! TTTTTGCGTATGTCCCTATG+GGG - chr8_1:45750760-45750779 None:intergenic 40.0%
! CTTTTTGCGTATGTCCCTAT+GGG - chr8_1:45750761-45750780 None:intergenic 40.0%
CATACGCAAAAAGGACAGTA+TGG + chr8_1:45750768-45750787 Msa1196480:five_prime_UTR 40.0%
GGGAGACAAGTGAATGAAAT+AGG - chr8_1:45750809-45750828 None:intergenic 40.0%
!! TTTGAGAAAGGTATTGGGCT+AGG - chr8_1:45750860-45750879 None:intergenic 40.0%
TGTTGAGAGAATGTGTCAGT+TGG - chr8_1:45750910-45750929 None:intergenic 40.0%
GTAGCCATGAAAGAGAGATT+AGG - chr8_1:45750934-45750953 None:intergenic 40.0%
TAGAGTCTAGGTAGCTACTT+AGG - chr8_1:45751084-45751103 None:intergenic 40.0%
TAAGTAGCTACCTAGACTCT+AGG + chr8_1:45751083-45751102 Msa1196480:five_prime_UTR 40.0%
TTGAAGGAAACCTAGAGTCT+AGG - chr8_1:45751096-45751115 None:intergenic 40.0%
AGATCTCGTTCAAGACAATC+TGG + chr8_1:45751269-45751288 Msa1196480:CDS 40.0%
AACTTGTACCTGAACTCTCA+AGG + chr8_1:45751348-45751367 Msa1196480:CDS 40.0%
TAGTGTGTGTTCGTTCTACA+GGG - chr8_1:45751723-45751742 None:intergenic 40.0%
AAACGAGTTAAGGGGATTCA+GGG - chr8_1:45752442-45752461 None:intergenic 40.0%
TAAACGAGTTAAGGGGATTC+AGG - chr8_1:45752443-45752462 None:intergenic 40.0%
TTGTGGGTAAACGAGTTAAG+GGG - chr8_1:45752450-45752469 None:intergenic 40.0%
GTTTGTGGGTAAACGAGTTA+AGG - chr8_1:45752452-45752471 None:intergenic 40.0%
TCGTTTACCCACAAACTTGT+AGG + chr8_1:45752456-45752475 Msa1196480:intron 40.0%
CGTTTACCCACAAACTTGTA+GGG + chr8_1:45752457-45752476 Msa1196480:intron 40.0%
TTTGTAGGTTTCAGCTGCTA+AGG + chr8_1:45752613-45752632 Msa1196480:intron 40.0%
! TGACCGATTGTCACAACTTT+TGG + chr8_1:45752694-45752713 Msa1196480:CDS 40.0%
AAAACGTTACCCATAAGGAG+AGG - chr8_1:45752876-45752895 None:intergenic 40.0%
ACACGAAAACGTTACCCATA+AGG - chr8_1:45752881-45752900 None:intergenic 40.0%
AGATCACAAACTTCATCCAG+TGG + chr8_1:45752947-45752966 Msa1196480:three_prime_UTR 40.0%
GTATGTCCCTATGGGGTTAA+AGG - chr8_1:45750753-45750772 None:intergenic 45.0%
! CCTTTTTGCGTATGTCCCTA+TGG - chr8_1:45750762-45750781 None:intergenic 45.0%
CCATAGGGACATACGCAAAA+AGG + chr8_1:45750759-45750778 Msa1196480:five_prime_UTR 45.0%
!! GTTGGTGTTGGAAGTTGAAG+AGG - chr8_1:45750892-45750911 None:intergenic 45.0%
! GAGAATGTGTCAGTTGGTGT+TGG - chr8_1:45750904-45750923 None:intergenic 45.0%
GATGGACGAGAGAGAGAAAA+GGG - chr8_1:45750956-45750975 None:intergenic 45.0%
! TGATGGACGAGAGAGAGAAA+AGG - chr8_1:45750957-45750976 None:intergenic 45.0%
TTCTCAGCTGAGACAAGTGA+TGG - chr8_1:45750974-45750993 None:intergenic 45.0%
TTGAGCATCAGAGATCTCAG+TGG - chr8_1:45751300-45751319 None:intergenic 45.0%
GTAGTGTGTGTTCGTTCTAC+AGG - chr8_1:45751724-45751743 None:intergenic 45.0%
! TTGAGCACTGTTAGGGTCTA+TGG - chr8_1:45752723-45752742 None:intergenic 45.0%
! TAGCTGCTTGAGCACTGTTA+GGG - chr8_1:45752730-45752749 None:intergenic 45.0%
! ATAGCTGCTTGAGCACTGTT+AGG - chr8_1:45752731-45752750 None:intergenic 45.0%
GTGCTCAAGCAGCTATCATT+AGG + chr8_1:45752735-45752754 Msa1196480:CDS 45.0%
GTATGTCCCTATGGGGTTAA+AGG - chr8_1:45750753-45750772 None:intergenic 45.0%
! CCTTTTTGCGTATGTCCCTA+TGG - chr8_1:45750762-45750781 None:intergenic 45.0%
CCATAGGGACATACGCAAAA+AGG + chr8_1:45750759-45750778 Msa1196480:five_prime_UTR 45.0%
!! GTTGGTGTTGGAAGTTGAAG+AGG - chr8_1:45750892-45750911 None:intergenic 45.0%
! GAGAATGTGTCAGTTGGTGT+TGG - chr8_1:45750904-45750923 None:intergenic 45.0%
GATGGACGAGAGAGAGAAAA+GGG - chr8_1:45750956-45750975 None:intergenic 45.0%
! TGATGGACGAGAGAGAGAAA+AGG - chr8_1:45750957-45750976 None:intergenic 45.0%
TTCTCAGCTGAGACAAGTGA+TGG - chr8_1:45750974-45750993 None:intergenic 45.0%
TTGAGCATCAGAGATCTCAG+TGG - chr8_1:45751300-45751319 None:intergenic 45.0%
GTAGTGTGTGTTCGTTCTAC+AGG - chr8_1:45751724-45751743 None:intergenic 45.0%
! TTGAGCACTGTTAGGGTCTA+TGG - chr8_1:45752723-45752742 None:intergenic 45.0%
! TAGCTGCTTGAGCACTGTTA+GGG - chr8_1:45752730-45752749 None:intergenic 45.0%
! ATAGCTGCTTGAGCACTGTT+AGG - chr8_1:45752731-45752750 None:intergenic 45.0%
GTGCTCAAGCAGCTATCATT+AGG + chr8_1:45752735-45752754 Msa1196480:CDS 45.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_1:45751791-45751810 None:intergenic 5.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_1:45751791-45751810 None:intergenic 5.0%
!! TTATAATAAATAAATGTTAA+TGG - chr8_1:45752578-45752597 None:intergenic 5.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_1:45751791-45751810 None:intergenic 5.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_1:45751791-45751810 None:intergenic 5.0%
!! TTATAATAAATAAATGTTAA+TGG - chr8_1:45752578-45752597 None:intergenic 5.0%
AAAGAGGTCCCCATGCAGTT+GGG + chr8_1:45750698-45750717 Msa1196480:five_prime_UTR 50.0%
! ACTCTCAAGGCGTTCCCATA+AGG + chr8_1:45751361-45751380 Msa1196480:CDS 50.0%
CCTCAGCCCTACAAGTTTGT+GGG - chr8_1:45752466-45752485 None:intergenic 50.0%
CCACAAACTTGTAGGGCTGA+GGG + chr8_1:45752464-45752483 Msa1196480:intron 50.0%
TCAGTGCATGAGACTTCCAC+TGG - chr8_1:45752966-45752985 None:intergenic 50.0%
ATGGGGACCTCTTTGTGTCA+GGG - chr8_1:45750692-45750711 None:intergenic 50.0%
AAAGAGGTCCCCATGCAGTT+GGG + chr8_1:45750698-45750717 Msa1196480:five_prime_UTR 50.0%
! ACTCTCAAGGCGTTCCCATA+AGG + chr8_1:45751361-45751380 Msa1196480:CDS 50.0%
CCTCAGCCCTACAAGTTTGT+GGG - chr8_1:45752466-45752485 None:intergenic 50.0%
CCACAAACTTGTAGGGCTGA+GGG + chr8_1:45752464-45752483 Msa1196480:intron 50.0%
TCAGTGCATGAGACTTCCAC+TGG - chr8_1:45752966-45752985 None:intergenic 50.0%
CAAAGAGGTCCCCATGCAGT+TGG + chr8_1:45750697-45750716 Msa1196480:five_prime_UTR 55.0%
AAGAGGTCCCCATGCAGTTG+GGG + chr8_1:45750699-45750718 Msa1196480:five_prime_UTR 55.0%
TAACACCCCCCCAACTGCAT+GGG - chr8_1:45750710-45750729 None:intergenic 55.0%
TGGGAACGCCTTGAGAGTTC+AGG - chr8_1:45751359-45751378 None:intergenic 55.0%
CCCACAAACTTGTAGGGCTG+AGG + chr8_1:45752463-45752482 Msa1196480:intron 55.0%
CCCTCAGCCCTACAAGTTTG+TGG - chr8_1:45752467-45752486 None:intergenic 55.0%
TGGGGACCTCTTTGTGTCAG+GGG - chr8_1:45750691-45750710 None:intergenic 55.0%
CATGGGGACCTCTTTGTGTC+AGG - chr8_1:45750693-45750712 None:intergenic 55.0%
CAAAGAGGTCCCCATGCAGT+TGG + chr8_1:45750697-45750716 Msa1196480:five_prime_UTR 55.0%
AAGAGGTCCCCATGCAGTTG+GGG + chr8_1:45750699-45750718 Msa1196480:five_prime_UTR 55.0%
TAACACCCCCCCAACTGCAT+GGG - chr8_1:45750710-45750729 None:intergenic 55.0%
TGGGAACGCCTTGAGAGTTC+AGG - chr8_1:45751359-45751378 None:intergenic 55.0%
CCCACAAACTTGTAGGGCTG+AGG + chr8_1:45752463-45752482 Msa1196480:intron 55.0%
CCCTCAGCCCTACAAGTTTG+TGG - chr8_1:45752467-45752486 None:intergenic 55.0%
AGAGGTCCCCATGCAGTTGG+GGG + chr8_1:45750700-45750719 Msa1196480:five_prime_UTR 60.0%
AACACCCCCCCAACTGCATG+GGG - chr8_1:45750709-45750728 None:intergenic 60.0%
GTAACACCCCCCCAACTGCA+TGG - chr8_1:45750711-45750730 None:intergenic 60.0%
AGAGGTCCCCATGCAGTTGG+GGG + chr8_1:45750700-45750719 Msa1196480:five_prime_UTR 60.0%
AACACCCCCCCAACTGCATG+GGG - chr8_1:45750709-45750728 None:intergenic 60.0%
GTAACACCCCCCCAACTGCA+TGG - chr8_1:45750711-45750730 None:intergenic 60.0%
GAGGTCCCCATGCAGTTGGG+GGG + chr8_1:45750701-45750720 Msa1196480:five_prime_UTR 65.0%
AGGTCCCCATGCAGTTGGGG+GGG + chr8_1:45750702-45750721 Msa1196480:five_prime_UTR 65.0%
GAGGTCCCCATGCAGTTGGG+GGG + chr8_1:45750701-45750720 Msa1196480:five_prime_UTR 65.0%
AGGTCCCCATGCAGTTGGGG+GGG + chr8_1:45750702-45750721 Msa1196480:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 45750691 45752989 45750691 ID=Msa1196480;Name=Msa1196480
chr8_1 mRNA 45750691 45752989 45750691 ID=Msa1196480-mRNA-1;Parent=Msa1196480;Name=Msa1196480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=566|1|1|1|1|1|2|217|92
chr8_1 exon 45750691 45751382 45750691 ID=Msa1196480-mRNA-1:exon:14597;Parent=Msa1196480-mRNA-1
chr8_1 exon 45752620 45752989 45752620 ID=Msa1196480-mRNA-1:exon:14598;Parent=Msa1196480-mRNA-1
chr8_1 five_prime_UTR 45750691 45751256 45750691 ID=Msa1196480-mRNA-1:five_prime_utr;Parent=Msa1196480-mRNA-1
chr8_1 CDS 45751257 45751382 45751257 ID=Msa1196480-mRNA-1:cds;Parent=Msa1196480-mRNA-1
chr8_1 CDS 45752620 45752772 45752620 ID=Msa1196480-mRNA-1:cds;Parent=Msa1196480-mRNA-1
chr8_1 three_prime_UTR 45752773 45752989 45752773 ID=Msa1196480-mRNA-1:three_prime_utr;Parent=Msa1196480-mRNA-1
Gene Sequence

>Msa1196480

ATGTCTAGCAGAAGATCTCGTTCAAGACAATCTGGTGTTTCCACTGAGATCTCTGATGCTCAAATTAATGATCTCGTCACCAAGTTACAACAACTTGTACCTGAACTCTCAAGGCGTTCCCATAAGGTTTCAGCTGCTAAGGTATTGCAAGAGACTTGCAATTACATCAAAAACTTGCATAGAGAGGTTGATGATCTTAGTGACCGATTGTCACAACTTTTGGACACCATAGACCCTAACAGTGCTCAAGCAGCTATCATTAGGAGCTTACTTATGTGA
Protein sequence

>Msa1196480

MSSRRSRSRQSGVSTEISDAQINDLVTKLQQLVPELSRRSHKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLDTIDPNSAQAAIIRSLLM*