AlfalfaGEDB | Msa1196480

M. sativa cultivar zhongmu-4 / Msa1196480

KEGG: -
GO: GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0050896
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196480	sp\|F4JCN9\|PRE4_ARATH	69.149	94	24	3	1	92	1	91	3.24e-35	118

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196480	A0A396GKL5	98.913	92	1	0	1	92	1	92	8.61e-58	181

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TFs/TRs:

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Gene ID	Type	Classification
Msa1196480	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1196480	Msa1247390	0.815108	1.094392e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196480	MtrunA17_Chr8g0362131	98.913	92	1	0	1	92	1	92	1.66e-61	181
Msa1196480	MtrunA17_Chr5g0416431	82.051	78	13	1	16	92	16	93	1.64e-39	126
Msa1196480	MtrunA17_Chr7g0217141	76.087	92	20	2	1	91	1	91	4.65e-38	122
Msa1196480	MtrunA17_Chr6g0453041	75.000	76	18	1	17	91	15	90	6.74e-32	107
Msa1196480	MtrunA17_Chr3g0093001	71.429	77	19	2	17	92	17	91	2.98e-30	102
Msa1196480	MtrunA17_Chr7g0237771	50.617	81	34	3	1	80	1	76	4.46e-13	59.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1196480	AT3G47710.1	69.149	94	24	3	1	92	1	91	3.30e-36	118
Msa1196480	AT3G28857.1	65.957	94	28	2	1	92	1	92	8.19e-36	117
Msa1196480	AT1G26945.1	78.378	74	15	1	19	91	19	92	1.29e-33	111
Msa1196480	AT5G15160.1	68.000	75	22	1	19	91	18	92	1.51e-31	106
Msa1196480	AT1G74500.1	74.026	77	16	2	17	91	17	91	6.80e-31	104
Msa1196480	AT5G39860.1	66.667	78	24	1	17	92	15	92	8.50e-31	104

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Find 58 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTTACCCATAAGGAG+AGG	0.710641	8_1:-45752873	None:intergenic
AAAGAGGTCCCCATGCAGTT+GGG	0.423561	8_1:+45750698	Msa1196480:five_prime_UTR
AAAGTGTTTGAGAAAGGTAT+TGG	0.351552	8_1:-45750863	None:intergenic
AACTTGTACCTGAACTCTCA+AGG	0.607839	8_1:+45751348	Msa1196480:CDS
AAGAGGTCCCCATGCAGTTG+GGG	0.496582	8_1:+45750699	Msa1196480:five_prime_UTR
AAGTGTTTGAGAAAGGTATT+GGG	0.430347	8_1:-45750862	None:intergenic
ACAACCTAATCTCTCTTTCA+TGG	0.408857	8_1:+45750927	Msa1196480:five_prime_UTR
ACAAGTGAATGAAATAGGAA+TGG	0.501907	8_1:-45750801	None:intergenic
ACACGAAAACGTTACCCATA+AGG	0.478677	8_1:-45752878	None:intergenic
ACATTTCCTTTAACCCCATA+GGG	0.527841	8_1:+45750744	Msa1196480:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAAGTGAATGAAATAGGAAT+GGG	-	chr8_1:45750803-45750822	None:intergenic	30.0%
ACAAGTGAATGAAATAGGAA+TGG	-	chr8_1:45750804-45750823	None:intergenic	30.0%
TAAATAGGGATATTACGTGA+GGG	-	chr8_1:45750829-45750848	None:intergenic	30.0%
TTAAATAGGGATATTACGTG+AGG	-	chr8_1:45750830-45750849	None:intergenic	30.0%
GCTAGGCAAGTTATTAAATA+GGG	-	chr8_1:45750843-45750862	None:intergenic	30.0%
GGATGAAAATGACAATAGTT+TGG	-	chr8_1:45751770-45751789	None:intergenic	30.0%
GTCAACAAAAAACTTTAGCT+TGG	-	chr8_1:45752250-45752269	None:intergenic	30.0%
ATGATATATGCATTGCAAGT+GGG	-	chr8_1:45752501-45752520	None:intergenic	30.0%
AATGATATATGCATTGCAAG+TGG	-	chr8_1:45752502-45752521	None:intergenic	30.0%
AGCTAGTGAGACATATTAAA+AGG	-	chr8_1:45752822-45752841	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	45750691	45752989	45750691	ID=Msa1196480;Name=Msa1196480
chr8_1	mRNA	45750691	45752989	45750691	ID=Msa1196480-mRNA-1;Parent=Msa1196480;Name=Msa1196480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=566\|1\|1\|1\|1\|1\|2\|217\|92
chr8_1	exon	45750691	45751382	45750691	ID=Msa1196480-mRNA-1:exon:14597;Parent=Msa1196480-mRNA-1
chr8_1	exon	45752620	45752989	45752620	ID=Msa1196480-mRNA-1:exon:14598;Parent=Msa1196480-mRNA-1
chr8_1	five_prime_UTR	45750691	45751256	45750691	ID=Msa1196480-mRNA-1:five_prime_utr;Parent=Msa1196480-mRNA-1
chr8_1	CDS	45751257	45751382	45751257	ID=Msa1196480-mRNA-1:cds;Parent=Msa1196480-mRNA-1
chr8_1	CDS	45752620	45752772	45752620	ID=Msa1196480-mRNA-1:cds;Parent=Msa1196480-mRNA-1
chr8_1	three_prime_UTR	45752773	45752989	45752773	ID=Msa1196480-mRNA-1:three_prime_utr;Parent=Msa1196480-mRNA-1

Gene Sequence	>Msa1196480 ATGTCTAGCAGAAGATCTCGTTCAAGACAATCTGGTGTTTCCACTGAGATCTCTGATGCTCAAATTAATGATCTCGTCACCAAGTTACAACAACTTGTACCTGAACTCTCAAGGCGTTCCCATAAGGTTTCAGCTGCTAAGGTATTGCAAGAGACTTGCAATTACATCAAAAACTTGCATAGAGAGGTTGATGATCTTAGTGACCGATTGTCACAACTTTTGGACACCATAGACCCTAACAGTGCTCAAGCAGCTATCATTAGGAGCTTACTTATGTGA
Protein sequence	>Msa1196480 MSSRRSRSRQSGVSTEISDAQINDLVTKLQQLVPELSRRSHKVSAAKVLQETCNYIKNLHREVDDLSDRLSQLLDTIDPNSAQAAIIRSLLM*