AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1247390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCGATTGTCACAACTTT+TGG 0.247784 8_2:+40242599 Msa1247390:three_prime_UTR
GGCTAGGCAAGTTATTAAAT+AGG 0.271481 8_2:-40241037 None:intergenic
TTATCATCAACCTCTCCTTA+TGG 0.309062 8_2:+40242765 Msa1247390:three_prime_UTR
TTGAGCACTGTTAGGGTCTA+TGG 0.329793 8_2:-40242625 None:intergenic
AGCTAGTGAGACATATTAAA+AGG 0.335554 8_2:-40242724 None:intergenic
AAAGTGTTTGAGAAAGGTAT+TGG 0.351552 8_2:-40241059 None:intergenic
ATAGCTGCTTGAGCACTGTT+AGG 0.352323 8_2:-40242633 None:intergenic
TTACAAAATGTCTAAAGAAC+GGG 0.367735 8_2:-40242899 None:intergenic
GGGAGACAAGTGAATGAAAT+AGG 0.380265 8_2:-40241002 None:intergenic
TATCATCAACCTCTCCTTAT+GGG 0.387409 8_2:+40242766 Msa1247390:three_prime_UTR
TAGTGTTAATTAGTGTGAAA+TGG 0.390486 8_2:+40242836 Msa1247390:three_prime_UTR
GCTAGGCAAGTTATTAAATA+GGG 0.401242 8_2:-40241036 None:intergenic
TGGGAACGCCTTGAGAGTTC+AGG 0.406573 8_2:-40241498 None:intergenic
ACAACCTAATCTCTCTTTCA+TGG 0.408857 8_2:+40241123 Msa1247390:CDS
AGAAAATTATTATACCTTAT+GGG 0.418461 8_2:-40241517 None:intergenic
GATGGACGAGAGAGAGAAAA+GGG 0.419581 8_2:-40241149 None:intergenic
GTGCTCAAGCAGCTATCATT+AGG 0.423047 8_2:+40242640 Msa1247390:three_prime_UTR
TCTTCTGCTAGACATTGTTA+AGG 0.430216 8_2:-40241447 None:intergenic
AAGTGTTTGAGAAAGGTATT+GGG 0.430347 8_2:-40241058 None:intergenic
AAACGAAAACGTTACCCATA+AGG 0.478677 8_2:-40242780 None:intergenic
AGGATAAAAGTGTTTGAGAA+AGG 0.482084 8_2:-40241065 None:intergenic
AGGTACAAGTTGTTGTAACT+TGG 0.482816 8_2:-40241478 None:intergenic
ACTCTCAAGGCGTTCCCATA+AGG 0.483554 8_2:+40241503 Msa1247390:three_prime_UTR
TTTGAGAAAGGTATTGGGCT+AGG 0.496830 8_2:-40241053 None:intergenic
TGATGGACGAGAGAGAGAAA+AGG 0.497265 8_2:-40241150 None:intergenic
ACAAGTGAATGAAATAGGAA+TGG 0.501907 8_2:-40240997 None:intergenic
TAGAGTCTAGGTAGCTACTT+AGG 0.512562 8_2:-40241276 None:intergenic
ATTGTAGGTTTCAGCTGCTA+AGG 0.515798 8_2:+40242518 Msa1247390:intron
TTCTCAGCTGAGACAAGTGA+TGG 0.516764 8_2:-40241167 None:intergenic
CAAGTGAATGAAATAGGAAT+GGG 0.519289 8_2:-40240996 None:intergenic
TAGCTGCTTGAGCACTGTTA+GGG 0.522205 8_2:-40242632 None:intergenic
TTGAAGGAAACCTAGAGTCT+AGG 0.523348 8_2:-40241288 None:intergenic
GTAGCCATGAAAGAGAGATT+AGG 0.531428 8_2:-40241127 None:intergenic
TAAGTAGCTACCTAGACTCT+AGG 0.537832 8_2:+40241278 Msa1247390:exon
GAGAATGTGTCAGTTGGTGT+TGG 0.544938 8_2:-40241097 None:intergenic
TGTCCAAAAGTTGTGACAAT+CGG 0.545364 8_2:-40242602 None:intergenic
GTTACAAAATGTCTAAAGAA+CGG 0.552336 8_2:-40242900 None:intergenic
GTTGGTGTTGGAAGTTGAAG+AGG 0.564686 8_2:-40241085 None:intergenic
TTAAATAGGGATATTACGTG+AGG 0.596488 8_2:-40241023 None:intergenic
AACTTGTACCTGAACTCTCA+AGG 0.607839 8_2:+40241490 Msa1247390:three_prime_UTR
TGTTGAGAGAATGTGTCAGT+TGG 0.622998 8_2:-40241103 None:intergenic
GAGACATATTAAAAGGCATG+TGG 0.632495 8_2:-40242717 None:intergenic
TAAATAGGGATATTACGTGA+GGG 0.635260 8_2:-40241022 None:intergenic
CAGTGCATGAGACTTCCACT+AGG 0.648690 8_2:-40242874 None:intergenic
AGATCACAAACTTCACCTAG+TGG 0.660347 8_2:+40242859 Msa1247390:three_prime_UTR
TACAAAATGTCTAAAGAACG+GGG 0.685856 8_2:-40242898 None:intergenic
AAAACGTTACCCATAAGGAG+AGG 0.703821 8_2:-40242775 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAAAATTATTATACCTTAT+GGG - chr8_2:40241520-40241539 None:intergenic 15.0%
!! AAGAAAATTATTATACCTTA+TGG - chr8_2:40241521-40241540 None:intergenic 15.0%
!!! AAATCAATTTTATGTGTTTA+GGG - chr8_2:40241621-40241640 None:intergenic 15.0%
!!! AAATTGATTTCTAAATGATA+TGG + chr8_2:40241631-40241650 Msa1247390:intron 15.0%
!!! AAAATGATCTTTAATTTTTC+TGG + chr8_2:40241951-40241970 Msa1247390:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_2:40242073-40242092 Msa1247390:intron 15.0%
!! TTTATTATTGTATGTATTGT+AGG + chr8_2:40242503-40242522 Msa1247390:intron 15.0%
!! AGAAAATTATTATACCTTAT+GGG - chr8_2:40241520-40241539 None:intergenic 15.0%
!! AAGAAAATTATTATACCTTA+TGG - chr8_2:40241521-40241540 None:intergenic 15.0%
!!! AAATCAATTTTATGTGTTTA+GGG - chr8_2:40241621-40241640 None:intergenic 15.0%
!!! AAATTGATTTCTAAATGATA+TGG + chr8_2:40241631-40241650 Msa1247390:intron 15.0%
!!! AAAATGATCTTTAATTTTTC+TGG + chr8_2:40241951-40241970 Msa1247390:intron 15.0%
!!! TAAAAACATATCTTCAATTT+TGG + chr8_2:40242073-40242092 Msa1247390:intron 15.0%
!! TTTATTATTGTATGTATTGT+AGG + chr8_2:40242503-40242522 Msa1247390:intron 15.0%
!!! GAAATCAATTTTATGTGTTT+AGG - chr8_2:40241622-40241641 None:intergenic 20.0%
!!! TTTTGAATGACATAAATTTG+AGG - chr8_2:40241831-40241850 None:intergenic 20.0%
!! TTTATGTCATTCAAAATCAA+TGG + chr8_2:40241835-40241854 Msa1247390:intron 20.0%
!!! GAAATCAATTTTATGTGTTT+AGG - chr8_2:40241622-40241641 None:intergenic 20.0%
!!! TTTTGAATGACATAAATTTG+AGG - chr8_2:40241831-40241850 None:intergenic 20.0%
!! TTTATGTCATTCAAAATCAA+TGG + chr8_2:40241835-40241854 Msa1247390:intron 20.0%
!! TAGACATTGTTAAGGTTTTT+GGG - chr8_2:40241442-40241461 None:intergenic 25.0%
! TAAAAGAGAGAATACAGAAA+AGG - chr8_2:40241545-40241564 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_2:40241979-40241998 None:intergenic 25.0%
!!! AAATTTGTTGTGTTCAAGAA+AGG + chr8_2:40242198-40242217 Msa1247390:intron 25.0%
!!! AATTTGTTGTGTTCAAGAAA+GGG + chr8_2:40242199-40242218 Msa1247390:intron 25.0%
! TAGTGTTAATTAGTGTGAAA+TGG + chr8_2:40242836-40242855 Msa1247390:three_prime_UTR 25.0%
! TTACAAAATGTCTAAAGAAC+GGG - chr8_2:40242902-40242921 None:intergenic 25.0%
! GTTACAAAATGTCTAAAGAA+CGG - chr8_2:40242903-40242922 None:intergenic 25.0%
!! TAGACATTGTTAAGGTTTTT+GGG - chr8_2:40241442-40241461 None:intergenic 25.0%
! TAAAAGAGAGAATACAGAAA+AGG - chr8_2:40241545-40241564 None:intergenic 25.0%
! AGTTCTTGAAATTATTACGT+AGG - chr8_2:40241979-40241998 None:intergenic 25.0%
!!! AAATTTGTTGTGTTCAAGAA+AGG + chr8_2:40242198-40242217 Msa1247390:intron 25.0%
!!! AATTTGTTGTGTTCAAGAAA+GGG + chr8_2:40242199-40242218 Msa1247390:intron 25.0%
! TAGTGTTAATTAGTGTGAAA+TGG + chr8_2:40242836-40242855 Msa1247390:three_prime_UTR 25.0%
! TTACAAAATGTCTAAAGAAC+GGG - chr8_2:40242902-40242921 None:intergenic 25.0%
! GTTACAAAATGTCTAAAGAA+CGG - chr8_2:40242903-40242922 None:intergenic 25.0%
CAAGTGAATGAAATAGGAAT+GGG - chr8_2:40240999-40241018 None:intergenic 30.0%
ACAAGTGAATGAAATAGGAA+TGG - chr8_2:40241000-40241019 None:intergenic 30.0%
TAAATAGGGATATTACGTGA+GGG - chr8_2:40241025-40241044 None:intergenic 30.0%
TTAAATAGGGATATTACGTG+AGG - chr8_2:40241026-40241045 None:intergenic 30.0%
GCTAGGCAAGTTATTAAATA+GGG - chr8_2:40241039-40241058 None:intergenic 30.0%
!! AAGTGTTTGAGAAAGGTATT+GGG - chr8_2:40241061-40241080 None:intergenic 30.0%
! AAAGTGTTTGAGAAAGGTAT+TGG - chr8_2:40241062-40241081 None:intergenic 30.0%
!! AGGATAAAAGTGTTTGAGAA+AGG - chr8_2:40241068-40241087 None:intergenic 30.0%
! AGACATTGTTAAGGTTTTTG+GGG - chr8_2:40241441-40241460 None:intergenic 30.0%
! CTAGACATTGTTAAGGTTTT+TGG - chr8_2:40241443-40241462 None:intergenic 30.0%
GTCAACAAAAAACTTTAGCT+TGG - chr8_2:40242151-40242170 None:intergenic 30.0%
! CTACACACATATTTTATGTG+TGG + chr8_2:40242279-40242298 Msa1247390:intron 30.0%
! ACATATTTTATGTGTGGCTT+TGG + chr8_2:40242285-40242304 Msa1247390:intron 30.0%
ATGATATATGCATTGCAAGT+GGG - chr8_2:40242402-40242421 None:intergenic 30.0%
AATGATATATGCATTGCAAG+TGG - chr8_2:40242403-40242422 None:intergenic 30.0%
! CAACAAATTTTCAGTTGCTA+TGG - chr8_2:40242442-40242461 None:intergenic 30.0%
AGCTAGTGAGACATATTAAA+AGG - chr8_2:40242727-40242746 None:intergenic 30.0%
TACAAAATGTCTAAAGAACG+GGG - chr8_2:40242901-40242920 None:intergenic 30.0%
AAGTGAATGAAATAGGAATG+GGG - chr8_2:40240998-40241017 None:intergenic 30.0%
CAAGTGAATGAAATAGGAAT+GGG - chr8_2:40240999-40241018 None:intergenic 30.0%
ACAAGTGAATGAAATAGGAA+TGG - chr8_2:40241000-40241019 None:intergenic 30.0%
TAAATAGGGATATTACGTGA+GGG - chr8_2:40241025-40241044 None:intergenic 30.0%
TTAAATAGGGATATTACGTG+AGG - chr8_2:40241026-40241045 None:intergenic 30.0%
GCTAGGCAAGTTATTAAATA+GGG - chr8_2:40241039-40241058 None:intergenic 30.0%
!! AAGTGTTTGAGAAAGGTATT+GGG - chr8_2:40241061-40241080 None:intergenic 30.0%
! AAAGTGTTTGAGAAAGGTAT+TGG - chr8_2:40241062-40241081 None:intergenic 30.0%
!! AGGATAAAAGTGTTTGAGAA+AGG - chr8_2:40241068-40241087 None:intergenic 30.0%
! AGACATTGTTAAGGTTTTTG+GGG - chr8_2:40241441-40241460 None:intergenic 30.0%
! CTAGACATTGTTAAGGTTTT+TGG - chr8_2:40241443-40241462 None:intergenic 30.0%
GTCAACAAAAAACTTTAGCT+TGG - chr8_2:40242151-40242170 None:intergenic 30.0%
! CTACACACATATTTTATGTG+TGG + chr8_2:40242279-40242298 Msa1247390:intron 30.0%
! ACATATTTTATGTGTGGCTT+TGG + chr8_2:40242285-40242304 Msa1247390:intron 30.0%
ATGATATATGCATTGCAAGT+GGG - chr8_2:40242402-40242421 None:intergenic 30.0%
AATGATATATGCATTGCAAG+TGG - chr8_2:40242403-40242422 None:intergenic 30.0%
! CAACAAATTTTCAGTTGCTA+TGG - chr8_2:40242442-40242461 None:intergenic 30.0%
AGCTAGTGAGACATATTAAA+AGG - chr8_2:40242727-40242746 None:intergenic 30.0%
TACAAAATGTCTAAAGAACG+GGG - chr8_2:40242901-40242920 None:intergenic 30.0%
GGCTAGGCAAGTTATTAAAT+AGG - chr8_2:40241040-40241059 None:intergenic 35.0%
ACAACCTAATCTCTCTTTCA+TGG + chr8_2:40241123-40241142 Msa1247390:CDS 35.0%
!!! ATTGTTAAGGTTTTTGGGGT+AGG - chr8_2:40241437-40241456 None:intergenic 35.0%
TCTTCTGCTAGACATTGTTA+AGG - chr8_2:40241450-40241469 None:intergenic 35.0%
AGGTACAAGTTGTTGTAACT+TGG - chr8_2:40241481-40241500 None:intergenic 35.0%
!!! ATTTTATGTGTGGCTTTGGT+TGG + chr8_2:40242289-40242308 Msa1247390:intron 35.0%
TTTGTGGGTAAACGAGTTAA+GGG - chr8_2:40242352-40242371 None:intergenic 35.0%
CATCAAAAACTTGCATAGAG+AGG + chr8_2:40242563-40242582 Msa1247390:three_prime_UTR 35.0%
TGTCCAAAAGTTGTGACAAT+CGG - chr8_2:40242605-40242624 None:intergenic 35.0%
! GAGACATATTAAAAGGCATG+TGG - chr8_2:40242720-40242739 None:intergenic 35.0%
TTATCATCAACCTCTCCTTA+TGG + chr8_2:40242765-40242784 Msa1247390:three_prime_UTR 35.0%
TATCATCAACCTCTCCTTAT+GGG + chr8_2:40242766-40242785 Msa1247390:three_prime_UTR 35.0%
AAACGAAAACGTTACCCATA+AGG - chr8_2:40242783-40242802 None:intergenic 35.0%
GGCTAGGCAAGTTATTAAAT+AGG - chr8_2:40241040-40241059 None:intergenic 35.0%
ACAACCTAATCTCTCTTTCA+TGG + chr8_2:40241123-40241142 Msa1247390:CDS 35.0%
!!! ATTGTTAAGGTTTTTGGGGT+AGG - chr8_2:40241437-40241456 None:intergenic 35.0%
TCTTCTGCTAGACATTGTTA+AGG - chr8_2:40241450-40241469 None:intergenic 35.0%
AGGTACAAGTTGTTGTAACT+TGG - chr8_2:40241481-40241500 None:intergenic 35.0%
!!! ATTTTATGTGTGGCTTTGGT+TGG + chr8_2:40242289-40242308 Msa1247390:intron 35.0%
TTTGTGGGTAAACGAGTTAA+GGG - chr8_2:40242352-40242371 None:intergenic 35.0%
CATCAAAAACTTGCATAGAG+AGG + chr8_2:40242563-40242582 Msa1247390:three_prime_UTR 35.0%
TGTCCAAAAGTTGTGACAAT+CGG - chr8_2:40242605-40242624 None:intergenic 35.0%
! GAGACATATTAAAAGGCATG+TGG - chr8_2:40242720-40242739 None:intergenic 35.0%
TTATCATCAACCTCTCCTTA+TGG + chr8_2:40242765-40242784 Msa1247390:three_prime_UTR 35.0%
TATCATCAACCTCTCCTTAT+GGG + chr8_2:40242766-40242785 Msa1247390:three_prime_UTR 35.0%
AAACGAAAACGTTACCCATA+AGG - chr8_2:40242783-40242802 None:intergenic 35.0%
GGGAGACAAGTGAATGAAAT+AGG - chr8_2:40241005-40241024 None:intergenic 40.0%
!! TTTGAGAAAGGTATTGGGCT+AGG - chr8_2:40241056-40241075 None:intergenic 40.0%
TGTTGAGAGAATGTGTCAGT+TGG - chr8_2:40241106-40241125 None:intergenic 40.0%
GTAGCCATGAAAGAGAGATT+AGG - chr8_2:40241130-40241149 None:intergenic 40.0%
TAGAGTCTAGGTAGCTACTT+AGG - chr8_2:40241279-40241298 None:intergenic 40.0%
TAAGTAGCTACCTAGACTCT+AGG + chr8_2:40241278-40241297 Msa1247390:exon 40.0%
TTGAAGGAAACCTAGAGTCT+AGG - chr8_2:40241291-40241310 None:intergenic 40.0%
!!! AAGGTTTTTGGGGTAGGTAT+AGG - chr8_2:40241431-40241450 None:intergenic 40.0%
AACTTGTACCTGAACTCTCA+AGG + chr8_2:40241490-40241509 Msa1247390:three_prime_UTR 40.0%
TAGTGTGTGTTCGTTCTACA+GGG - chr8_2:40241865-40241884 None:intergenic 40.0%
AAACGAGTTAAGGGGATTCA+GGG - chr8_2:40242343-40242362 None:intergenic 40.0%
TAAACGAGTTAAGGGGATTC+AGG - chr8_2:40242344-40242363 None:intergenic 40.0%
TTGTGGGTAAACGAGTTAAG+GGG - chr8_2:40242351-40242370 None:intergenic 40.0%
GTTTGTGGGTAAACGAGTTA+AGG - chr8_2:40242353-40242372 None:intergenic 40.0%
TCGTTTACCCACAAACTTGT+AGG + chr8_2:40242357-40242376 Msa1247390:intron 40.0%
CGTTTACCCACAAACTTGTA+GGG + chr8_2:40242358-40242377 Msa1247390:intron 40.0%
ATTGTAGGTTTCAGCTGCTA+AGG + chr8_2:40242518-40242537 Msa1247390:intron 40.0%
! TGACCGATTGTCACAACTTT+TGG + chr8_2:40242599-40242618 Msa1247390:three_prime_UTR 40.0%
AAAACGTTACCCATAAGGAG+AGG - chr8_2:40242778-40242797 None:intergenic 40.0%
AGATCACAAACTTCACCTAG+TGG + chr8_2:40242859-40242878 Msa1247390:three_prime_UTR 40.0%
GGGAGACAAGTGAATGAAAT+AGG - chr8_2:40241005-40241024 None:intergenic 40.0%
!! TTTGAGAAAGGTATTGGGCT+AGG - chr8_2:40241056-40241075 None:intergenic 40.0%
TGTTGAGAGAATGTGTCAGT+TGG - chr8_2:40241106-40241125 None:intergenic 40.0%
GTAGCCATGAAAGAGAGATT+AGG - chr8_2:40241130-40241149 None:intergenic 40.0%
TAGAGTCTAGGTAGCTACTT+AGG - chr8_2:40241279-40241298 None:intergenic 40.0%
TAAGTAGCTACCTAGACTCT+AGG + chr8_2:40241278-40241297 Msa1247390:exon 40.0%
TTGAAGGAAACCTAGAGTCT+AGG - chr8_2:40241291-40241310 None:intergenic 40.0%
!!! AAGGTTTTTGGGGTAGGTAT+AGG - chr8_2:40241431-40241450 None:intergenic 40.0%
AACTTGTACCTGAACTCTCA+AGG + chr8_2:40241490-40241509 Msa1247390:three_prime_UTR 40.0%
TAGTGTGTGTTCGTTCTACA+GGG - chr8_2:40241865-40241884 None:intergenic 40.0%
AAACGAGTTAAGGGGATTCA+GGG - chr8_2:40242343-40242362 None:intergenic 40.0%
TAAACGAGTTAAGGGGATTC+AGG - chr8_2:40242344-40242363 None:intergenic 40.0%
TTGTGGGTAAACGAGTTAAG+GGG - chr8_2:40242351-40242370 None:intergenic 40.0%
GTTTGTGGGTAAACGAGTTA+AGG - chr8_2:40242353-40242372 None:intergenic 40.0%
TCGTTTACCCACAAACTTGT+AGG + chr8_2:40242357-40242376 Msa1247390:intron 40.0%
CGTTTACCCACAAACTTGTA+GGG + chr8_2:40242358-40242377 Msa1247390:intron 40.0%
ATTGTAGGTTTCAGCTGCTA+AGG + chr8_2:40242518-40242537 Msa1247390:intron 40.0%
! TGACCGATTGTCACAACTTT+TGG + chr8_2:40242599-40242618 Msa1247390:three_prime_UTR 40.0%
AAAACGTTACCCATAAGGAG+AGG - chr8_2:40242778-40242797 None:intergenic 40.0%
AGATCACAAACTTCACCTAG+TGG + chr8_2:40242859-40242878 Msa1247390:three_prime_UTR 40.0%
!! GTTGGTGTTGGAAGTTGAAG+AGG - chr8_2:40241088-40241107 None:intergenic 45.0%
! GAGAATGTGTCAGTTGGTGT+TGG - chr8_2:40241100-40241119 None:intergenic 45.0%
GATGGACGAGAGAGAGAAAA+GGG - chr8_2:40241152-40241171 None:intergenic 45.0%
! TGATGGACGAGAGAGAGAAA+AGG - chr8_2:40241153-40241172 None:intergenic 45.0%
TTCTCAGCTGAGACAAGTGA+TGG - chr8_2:40241170-40241189 None:intergenic 45.0%
GTAGTGTGTGTTCGTTCTAC+AGG - chr8_2:40241866-40241885 None:intergenic 45.0%
! TTGAGCACTGTTAGGGTCTA+TGG - chr8_2:40242628-40242647 None:intergenic 45.0%
! TAGCTGCTTGAGCACTGTTA+GGG - chr8_2:40242635-40242654 None:intergenic 45.0%
! ATAGCTGCTTGAGCACTGTT+AGG - chr8_2:40242636-40242655 None:intergenic 45.0%
GTGCTCAAGCAGCTATCATT+AGG + chr8_2:40242640-40242659 Msa1247390:three_prime_UTR 45.0%
!! GTTGGTGTTGGAAGTTGAAG+AGG - chr8_2:40241088-40241107 None:intergenic 45.0%
! GAGAATGTGTCAGTTGGTGT+TGG - chr8_2:40241100-40241119 None:intergenic 45.0%
GATGGACGAGAGAGAGAAAA+GGG - chr8_2:40241152-40241171 None:intergenic 45.0%
! TGATGGACGAGAGAGAGAAA+AGG - chr8_2:40241153-40241172 None:intergenic 45.0%
TTCTCAGCTGAGACAAGTGA+TGG - chr8_2:40241170-40241189 None:intergenic 45.0%
GTAGTGTGTGTTCGTTCTAC+AGG - chr8_2:40241866-40241885 None:intergenic 45.0%
! TTGAGCACTGTTAGGGTCTA+TGG - chr8_2:40242628-40242647 None:intergenic 45.0%
! TAGCTGCTTGAGCACTGTTA+GGG - chr8_2:40242635-40242654 None:intergenic 45.0%
! ATAGCTGCTTGAGCACTGTT+AGG - chr8_2:40242636-40242655 None:intergenic 45.0%
GTGCTCAAGCAGCTATCATT+AGG + chr8_2:40242640-40242659 Msa1247390:three_prime_UTR 45.0%
!!! TTTTTTTTTTTTTTTTTTGA+AGG - chr8_2:40241307-40241326 None:intergenic 5.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_2:40241933-40241952 None:intergenic 5.0%
!! TTATAATAAATAAATGTTAA+TGG - chr8_2:40242481-40242500 None:intergenic 5.0%
!!! TTTTTTTTTTTTTTTTTTGA+AGG - chr8_2:40241307-40241326 None:intergenic 5.0%
!! TTAAAATTTAATTAATTCTA+AGG - chr8_2:40241933-40241952 None:intergenic 5.0%
!! TTATAATAAATAAATGTTAA+TGG - chr8_2:40242481-40242500 None:intergenic 5.0%
! ACTCTCAAGGCGTTCCCATA+AGG + chr8_2:40241503-40241522 Msa1247390:three_prime_UTR 50.0%
CCTCAGCCCTACAAGTTTGT+GGG - chr8_2:40242367-40242386 None:intergenic 50.0%
CCACAAACTTGTAGGGCTGA+GGG + chr8_2:40242365-40242384 Msa1247390:intron 50.0%
ACTTGTAGGGCTGAGGGATT+CGG + chr8_2:40242371-40242390 Msa1247390:intron 50.0%
CAGTGCATGAGACTTCCACT+AGG - chr8_2:40242877-40242896 None:intergenic 50.0%
! ACTCTCAAGGCGTTCCCATA+AGG + chr8_2:40241503-40241522 Msa1247390:three_prime_UTR 50.0%
CCTCAGCCCTACAAGTTTGT+GGG - chr8_2:40242367-40242386 None:intergenic 50.0%
CCACAAACTTGTAGGGCTGA+GGG + chr8_2:40242365-40242384 Msa1247390:intron 50.0%
ACTTGTAGGGCTGAGGGATT+CGG + chr8_2:40242371-40242390 Msa1247390:intron 50.0%
CAGTGCATGAGACTTCCACT+AGG - chr8_2:40242877-40242896 None:intergenic 50.0%
TGGGAACGCCTTGAGAGTTC+AGG - chr8_2:40241501-40241520 None:intergenic 55.0%
CCCACAAACTTGTAGGGCTG+AGG + chr8_2:40242364-40242383 Msa1247390:intron 55.0%
CCCTCAGCCCTACAAGTTTG+TGG - chr8_2:40242368-40242387 None:intergenic 55.0%
TGGGAACGCCTTGAGAGTTC+AGG - chr8_2:40241501-40241520 None:intergenic 55.0%
CCCACAAACTTGTAGGGCTG+AGG + chr8_2:40242364-40242383 Msa1247390:intron 55.0%
CCCTCAGCCCTACAAGTTTG+TGG - chr8_2:40242368-40242387 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 40240996 40242922 40240996 ID=Msa1247390;Name=Msa1247390
chr8_2 mRNA 40240996 40242922 40240996 ID=Msa1247390-mRNA-1;Parent=Msa1247390;Name=Msa1247390-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.5|1|1|1|2|642|94
chr8_2 exon 40240996 40241524 40240996 ID=Msa1247390-mRNA-1:exon:12326;Parent=Msa1247390-mRNA-1
chr8_2 exon 40242525 40242922 40242525 ID=Msa1247390-mRNA-1:exon:12327;Parent=Msa1247390-mRNA-1
chr8_2 CDS 40240996 40241280 40240996 ID=Msa1247390-mRNA-1:cds;Parent=Msa1247390-mRNA-1
chr8_2 three_prime_UTR 40241281 40241524 40241281 ID=Msa1247390-mRNA-1:three_prime_utr;Parent=Msa1247390-mRNA-1
chr8_2 three_prime_UTR 40242525 40242922 40242525 ID=Msa1247390-mRNA-1:three_prime_utr;Parent=Msa1247390-mRNA-1
Gene Sequence

>Msa1247390

CCCATTCCTATTTCATTCACTTGTCTCCCTCACGTAATATCCCTATTTAATAACTTGCCTAGCCCAATACCTTTCTCAAACACTTTTATCCTCTTCAACTTCCAACACCAACTGACACATTCTCTCAACAACCTAATCTCTCTTTCATGGCTACCCTTTTCTCTCTCTCGTCCATCACTTGTCTCAGCTGAGAAAGAAAAAAAAAAATTACATTTTCTTTTTTTGCATTATTTTTTTCTTTCTTCTCTAAAATATTCACTTTATATAAGAGCTTCATATACCTAA
Protein sequence

>Msa1247390

PIPISFTCLPHVISLFNNLPSPIPFSNTFILFNFQHQLTHSLNNLISLSWLPFSLSRPSLVSAEKEKKKLHFLFLHYFFLSSLKYSLYIRASYT*