Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1197280 | KEH20050.1 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 7.17e-39 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1197280 | A0A072U2M1 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 3.42e-39 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1083300 | Msa1197280 | 0.801370 | 9.295281e-49 | -8.615850e-47 |
| Msa1197280 | Msa1250740 | 0.977365 | 1.234812e-143 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1197280 | MtrunA17_Chr8g0365951 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 6.74e-43 | 143 |
| Msa1197280 | MtrunA17_Chr5g0420931 | 79.167 | 72 | 15 | 0 | 3 | 74 | 119 | 190 | 1.79e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTGCAACCTCCTCTAGTC+AGG | 0.344591 | 8_1:-47510525 | Msa1197280:CDS |
| TGTTGCTATTGAAAATATAT+CGG | 0.363372 | 8_1:-47510485 | Msa1197280:CDS |
| CCACATGCTCTTGGCTCAGC+TGG | 0.376293 | 8_1:+47510550 | None:intergenic |
| TTCCTTTGTCTCCGACGACC+AGG | 0.432037 | 8_1:-47510358 | Msa1197280:CDS |
| CACAGCTCTGTGGCTTTCAC+TGG | 0.440184 | 8_1:+47510400 | None:intergenic |
| ATAACTCCAGAGTATAAGAT+TGG | 0.472140 | 8_1:-47510454 | Msa1197280:CDS |
| TGCACACAACCACATGCTCT+TGG | 0.481329 | 8_1:+47510541 | None:intergenic |
| TCCATGCCAATCTTATACTC+TGG | 0.493128 | 8_1:+47510448 | None:intergenic |
| TAAGATTGGCATGGAACCAT+TGG | 0.519535 | 8_1:-47510440 | Msa1197280:CDS |
| CTATTGAAAATATATCGGTT+CGG | 0.523106 | 8_1:-47510480 | Msa1197280:CDS |
| AGCTCTGTGGCTTTCACTGG+AGG | 0.537597 | 8_1:+47510403 | None:intergenic |
| TAGCAACATAAGGAGCAACT+TGG | 0.581394 | 8_1:+47510500 | None:intergenic |
| ACCCTGGTCGTCGGAGACAA+AGG | 0.583585 | 8_1:+47510356 | None:intergenic |
| TGTGCAACCTCCTCTAGTCA+GGG | 0.599233 | 8_1:-47510524 | Msa1197280:CDS |
| TCCTTTGTCTCCGACGACCA+GGG | 0.602253 | 8_1:-47510357 | Msa1197280:CDS |
| CCAGCTGAGCCAAGAGCATG+TGG | 0.608796 | 8_1:-47510550 | Msa1197280:CDS |
| GCAACTTGGTCCCTGACTAG+AGG | 0.627611 | 8_1:+47510514 | None:intergenic |
| TAAGAATTTACCCTGGTCGT+CGG | 0.629821 | 8_1:+47510347 | None:intergenic |
| TTTATCTCGCCACAGCTCTG+TGG | 0.632710 | 8_1:+47510390 | None:intergenic |
| TCCAGAGTATAAGATTGGCA+TGG | 0.639026 | 8_1:-47510449 | Msa1197280:CDS |
| CAGTGAAAGCCACAGAGCTG+TGG | 0.639575 | 8_1:-47510399 | Msa1197280:CDS |
| ACTTGGTCCCTGACTAGAGG+AGG | 0.659564 | 8_1:+47510517 | None:intergenic |
| TTACAGATAAGAATTTACCC+TGG | 0.684073 | 8_1:+47510340 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTCAATAGCAACATA+AGG | + | chr8_1:47510423-47510442 | None:intergenic | 20.0% |
| !!! | TGTTGCTATTGAAAATATAT+CGG | - | chr8_1:47510425-47510444 | Msa1197280:CDS | 20.0% |
| !! | CTATTGAAAATATATCGGTT+CGG | - | chr8_1:47510430-47510449 | Msa1197280:CDS | 25.0% |
| ATAACTCCAGAGTATAAGAT+TGG | - | chr8_1:47510456-47510475 | Msa1197280:CDS | 30.0% | |
| !! | GGTGTCTTCATTTTTACCAA+TGG | + | chr8_1:47510489-47510508 | None:intergenic | 35.0% |
| TAGCAACATAAGGAGCAACT+TGG | + | chr8_1:47510413-47510432 | None:intergenic | 40.0% | |
| TCCAGAGTATAAGATTGGCA+TGG | - | chr8_1:47510461-47510480 | Msa1197280:CDS | 40.0% | |
| TCCATGCCAATCTTATACTC+TGG | + | chr8_1:47510465-47510484 | None:intergenic | 40.0% | |
| TAAGATTGGCATGGAACCAT+TGG | - | chr8_1:47510470-47510489 | Msa1197280:CDS | 40.0% | |
| TGCACACAACCACATGCTCT+TGG | + | chr8_1:47510372-47510391 | None:intergenic | 50.0% | |
| TGTGCAACCTCCTCTAGTCA+GGG | - | chr8_1:47510386-47510405 | Msa1197280:CDS | 50.0% | |
| TTTATCTCGCCACAGCTCTG+TGG | + | chr8_1:47510523-47510542 | None:intergenic | 50.0% | |
| GTGTGCAACCTCCTCTAGTC+AGG | - | chr8_1:47510385-47510404 | Msa1197280:CDS | 55.0% | |
| ACTTGGTCCCTGACTAGAGG+AGG | + | chr8_1:47510396-47510415 | None:intergenic | 55.0% | |
| GCAACTTGGTCCCTGACTAG+AGG | + | chr8_1:47510399-47510418 | None:intergenic | 55.0% | |
| AGCTCTGTGGCTTTCACTGG+AGG | + | chr8_1:47510510-47510529 | None:intergenic | 55.0% | |
| CACAGCTCTGTGGCTTTCAC+TGG | + | chr8_1:47510513-47510532 | None:intergenic | 55.0% | |
| CAGTGAAAGCCACAGAGCTG+TGG | - | chr8_1:47510511-47510530 | Msa1197280:CDS | 55.0% | |
| TTCCTTTGTCTCCGACGACC+AGG | - | chr8_1:47510552-47510571 | Msa1197280:CDS | 55.0% | |
| TCCTTTGTCTCCGACGACCA+GGG | - | chr8_1:47510553-47510572 | Msa1197280:CDS | 55.0% | |
| CCACATGCTCTTGGCTCAGC+TGG | + | chr8_1:47510363-47510382 | None:intergenic | 60.0% | |
| ! | CCAGCTGAGCCAAGAGCATG+TGG | - | chr8_1:47510360-47510379 | Msa1197280:CDS | 60.0% |
| ACCCTGGTCGTCGGAGACAA+AGG | + | chr8_1:47510557-47510576 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 47510354 | 47510578 | 47510354 | ID=Msa1197280;Name=Msa1197280 |
| chr8_1 | mRNA | 47510354 | 47510578 | 47510354 | ID=Msa1197280-mRNA-1;Parent=Msa1197280;Name=Msa1197280-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|74 |
| chr8_1 | exon | 47510354 | 47510578 | 47510354 | ID=Msa1197280-mRNA-1:exon:15092;Parent=Msa1197280-mRNA-1 |
| chr8_1 | CDS | 47510354 | 47510578 | 47510354 | ID=Msa1197280-mRNA-1:cds;Parent=Msa1197280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
