Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1250740 | KEH20050.1 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 7.17e-39 | 141 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1250740 | A0A072U2M1 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 3.42e-39 | 141 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1197280 | Msa1250740 | 0.977365 | 1.234812e-143 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1250740 | MtrunA17_Chr8g0365951 | 88.889 | 72 | 8 | 0 | 3 | 74 | 204 | 275 | 6.74e-43 | 143 |
| Msa1250740 | MtrunA17_Chr5g0420931 | 79.167 | 72 | 15 | 0 | 3 | 74 | 119 | 190 | 1.79e-39 | 132 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTGCAACCTCCTCTAGTC+AGG | 0.344591 | 8_2:+46481084 | Msa1250740:CDS |
| TGTTGCTATTGAAAATATAT+CGG | 0.363372 | 8_2:+46481124 | Msa1250740:CDS |
| CCACATGCTCTTGGCTCAGC+TGG | 0.376293 | 8_2:-46481059 | None:intergenic |
| TTCCTTTGTCTCCGACGACC+AGG | 0.432037 | 8_2:+46481251 | Msa1250740:CDS |
| CACAGCTCTGTGGCTTTCAC+TGG | 0.440184 | 8_2:-46481209 | None:intergenic |
| ATAACTCCAGAGTATAAGAT+TGG | 0.472140 | 8_2:+46481155 | Msa1250740:CDS |
| TGCACACAACCACATGCTCT+TGG | 0.481329 | 8_2:-46481068 | None:intergenic |
| TCCATGCCAATCTTATACTC+TGG | 0.493128 | 8_2:-46481161 | None:intergenic |
| TAAGATTGGCATGGAACCAT+TGG | 0.519535 | 8_2:+46481169 | Msa1250740:CDS |
| CTATTGAAAATATATCGGTT+CGG | 0.523106 | 8_2:+46481129 | Msa1250740:CDS |
| AGCTCTGTGGCTTTCACTGG+AGG | 0.537597 | 8_2:-46481206 | None:intergenic |
| TAGCAACATAAGGAGCAACT+TGG | 0.581394 | 8_2:-46481109 | None:intergenic |
| ACCCTGGTCGTCGGAGACAA+AGG | 0.583585 | 8_2:-46481253 | None:intergenic |
| TGTGCAACCTCCTCTAGTCA+GGG | 0.599233 | 8_2:+46481085 | Msa1250740:CDS |
| TCCTTTGTCTCCGACGACCA+GGG | 0.602253 | 8_2:+46481252 | Msa1250740:CDS |
| CCAGCTGAGCCAAGAGCATG+TGG | 0.608796 | 8_2:+46481059 | Msa1250740:CDS |
| GCAACTTGGTCCCTGACTAG+AGG | 0.627611 | 8_2:-46481095 | None:intergenic |
| TAAGAATTTACCCTGGTCGT+CGG | 0.629821 | 8_2:-46481262 | None:intergenic |
| TTTATCTCGCCACAGCTCTG+TGG | 0.632710 | 8_2:-46481219 | None:intergenic |
| TCCAGAGTATAAGATTGGCA+TGG | 0.639026 | 8_2:+46481160 | Msa1250740:CDS |
| CAGTGAAAGCCACAGAGCTG+TGG | 0.639575 | 8_2:+46481210 | Msa1250740:CDS |
| ACTTGGTCCCTGACTAGAGG+AGG | 0.659564 | 8_2:-46481092 | None:intergenic |
| TTACAGATAAGAATTTACCC+TGG | 0.684073 | 8_2:-46481269 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTTCAATAGCAACATA+AGG | - | chr8_2:46481122-46481141 | None:intergenic | 20.0% |
| !!! | TGTTGCTATTGAAAATATAT+CGG | + | chr8_2:46481124-46481143 | Msa1250740:CDS | 20.0% |
| !! | CTATTGAAAATATATCGGTT+CGG | + | chr8_2:46481129-46481148 | Msa1250740:CDS | 25.0% |
| ATAACTCCAGAGTATAAGAT+TGG | + | chr8_2:46481155-46481174 | Msa1250740:CDS | 30.0% | |
| !! | GGTGTCTTCATTTTTACCAA+TGG | - | chr8_2:46481188-46481207 | None:intergenic | 35.0% |
| TAGCAACATAAGGAGCAACT+TGG | - | chr8_2:46481112-46481131 | None:intergenic | 40.0% | |
| TCCAGAGTATAAGATTGGCA+TGG | + | chr8_2:46481160-46481179 | Msa1250740:CDS | 40.0% | |
| TCCATGCCAATCTTATACTC+TGG | - | chr8_2:46481164-46481183 | None:intergenic | 40.0% | |
| TAAGATTGGCATGGAACCAT+TGG | + | chr8_2:46481169-46481188 | Msa1250740:CDS | 40.0% | |
| TGCACACAACCACATGCTCT+TGG | - | chr8_2:46481071-46481090 | None:intergenic | 50.0% | |
| TGTGCAACCTCCTCTAGTCA+GGG | + | chr8_2:46481085-46481104 | Msa1250740:CDS | 50.0% | |
| TTTATCTCGCCACAGCTCTG+TGG | - | chr8_2:46481222-46481241 | None:intergenic | 50.0% | |
| GTGTGCAACCTCCTCTAGTC+AGG | + | chr8_2:46481084-46481103 | Msa1250740:CDS | 55.0% | |
| ACTTGGTCCCTGACTAGAGG+AGG | - | chr8_2:46481095-46481114 | None:intergenic | 55.0% | |
| GCAACTTGGTCCCTGACTAG+AGG | - | chr8_2:46481098-46481117 | None:intergenic | 55.0% | |
| AGCTCTGTGGCTTTCACTGG+AGG | - | chr8_2:46481209-46481228 | None:intergenic | 55.0% | |
| CACAGCTCTGTGGCTTTCAC+TGG | - | chr8_2:46481212-46481231 | None:intergenic | 55.0% | |
| CAGTGAAAGCCACAGAGCTG+TGG | + | chr8_2:46481210-46481229 | Msa1250740:CDS | 55.0% | |
| TTCCTTTGTCTCCGACGACC+AGG | + | chr8_2:46481251-46481270 | Msa1250740:CDS | 55.0% | |
| TCCTTTGTCTCCGACGACCA+GGG | + | chr8_2:46481252-46481271 | Msa1250740:CDS | 55.0% | |
| CCACATGCTCTTGGCTCAGC+TGG | - | chr8_2:46481062-46481081 | None:intergenic | 60.0% | |
| ! | CCAGCTGAGCCAAGAGCATG+TGG | + | chr8_2:46481059-46481078 | Msa1250740:CDS | 60.0% |
| ACCCTGGTCGTCGGAGACAA+AGG | - | chr8_2:46481256-46481275 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 46481053 | 46481277 | 46481053 | ID=Msa1250740;Name=Msa1250740 |
| chr8_2 | mRNA | 46481053 | 46481277 | 46481053 | ID=Msa1250740-mRNA-1;Parent=Msa1250740;Name=Msa1250740-mRNA-1;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|74 |
| chr8_2 | exon | 46481053 | 46481277 | 46481053 | ID=Msa1250740-mRNA-1:exon:13967;Parent=Msa1250740-mRNA-1 |
| chr8_2 | CDS | 46481053 | 46481277 | 46481053 | ID=Msa1250740-mRNA-1:cds;Parent=Msa1250740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
