AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1197660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1197660 MtrunA17_Chr8g0366581 100.000 232 0 0 1 232 1 232 1.28e-177 486
Msa1197660 MtrunA17_Chr5g0411151 85.897 234 29 3 1 231 1 233 1.04e-147 411
Msa1197660 MtrunA17_Chr5g0395181 70.370 216 54 4 19 233 7 213 3.45e-108 310
Msa1197660 MtrunA17_Chr4g0040071 72.414 203 52 4 31 232 13 212 5.92e-104 299
Msa1197660 MtrunA17_Chr1g0179781 55.340 206 73 5 33 229 7 202 3.22e-73 221
Msa1197660 MtrunA17_Chr7g0265291 58.333 204 71 5 33 229 7 203 1.99e-71 216
Msa1197660 MtrunA17_Chr2g0308401 49.265 136 59 4 34 159 5 140 5.70e-39 132
Msa1197660 MtrunA17_Chr4g0020811 47.794 136 61 3 34 159 7 142 8.01e-39 132
Msa1197660 MtrunA17_Chr4g0065041 48.031 127 65 1 33 159 8 133 4.69e-37 130
Msa1197660 MtrunA17_Chr2g0297251 34.685 222 118 5 33 228 37 257 6.25e-37 130
Msa1197660 MtrunA17_Chr2g0320521 34.052 232 109 6 34 228 7 231 2.09e-36 128
Msa1197660 MtrunA17_Chr7g0230511 32.922 243 117 5 30 228 6 246 2.49e-36 128
Msa1197660 MtrunA17_Chr2g0320561 46.715 137 63 4 34 160 5 141 6.36e-32 117
Msa1197660 MtrunA17_Chr2g0320551 43.382 136 67 3 34 159 5 140 4.33e-31 112
Msa1197660 MtrunA17_Chr4g0052451 43.902 82 40 1 85 160 11 92 3.33e-20 82.0
Msa1197660 MtrunA17_Chr8g0392151 33.951 162 82 6 19 159 1 158 1.17e-16 76.3
Msa1197660 MtrunA17_Chr8g0386511 29.193 161 99 4 72 228 4 153 1.41e-15 71.6
Msa1197660 MtrunA17_Chr5g0434331 27.670 206 105 9 29 228 39 206 1.17e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1197660 AT4G17900.1 73.391 233 52 5 1 229 1 227 2.72e-122 347
Msa1197660 AT4G17900.2 72.906 203 46 4 30 229 17 213 6.39e-104 300
Msa1197660 AT1G32700.1 71.495 214 51 5 24 232 2 210 7.31e-102 294
Msa1197660 AT1G32700.2 71.023 176 42 4 61 232 1 171 2.53e-78 233
Msa1197660 AT5G46710.1 52.521 238 90 8 1 228 1 225 6.94e-78 234
Msa1197660 AT1G21000.2 50.209 239 82 4 26 228 6 243 6.90e-77 232
Msa1197660 AT1G21000.1 50.209 239 82 4 26 228 9 246 8.38e-77 232
Msa1197660 AT1G76590.1 50.877 228 80 4 33 228 18 245 2.11e-74 226
Msa1197660 AT5G46710.3 52.830 212 84 6 26 228 5 209 3.23e-72 219
Msa1197660 AT5G46710.2 59.627 161 57 3 1 159 1 155 1.71e-66 203
Msa1197660 AT1G43000.2 46.083 217 101 4 26 228 9 223 2.64e-62 194
Msa1197660 AT1G43000.1 46.083 217 101 4 26 228 2 216 3.53e-62 194
Msa1197660 AT2G27930.1 44.712 208 83 5 33 230 4 189 3.93e-53 170
Msa1197660 AT2G27930.4 44.712 208 83 5 33 230 4 189 3.93e-53 170
Msa1197660 AT2G27930.3 55.474 137 51 2 33 159 4 140 8.81e-49 158
Msa1197660 AT2G27930.2 55.474 137 51 2 33 159 4 140 8.81e-49 158
Msa1197660 AT2G12646.3 48.819 127 64 1 33 159 3 128 2.65e-37 131
Msa1197660 AT2G12646.1 48.819 127 64 1 33 159 6 131 2.82e-37 131
Msa1197660 AT2G12646.2 48.819 127 64 1 33 159 6 131 2.82e-37 131
Msa1197660 AT3G60670.1 37.949 195 105 3 28 221 2 181 2.09e-36 129
Msa1197660 AT1G31040.1 34.513 226 116 5 33 228 19 242 2.23e-34 123
Msa1197660 AT2G27930.5 45.455 154 53 4 86 230 61 192 5.89e-34 120
Msa1197660 AT2G27930.6 45.455 154 53 4 86 230 61 192 5.89e-34 120
Msa1197660 AT2G01818.1 34.579 214 115 9 35 228 13 221 3.75e-22 91.3
Msa1197660 AT2G01818.2 33.987 153 81 7 92 228 9 157 9.85e-11 58.9

Find 88 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGATCATCGTGCTATTC+AGG 0.214126 8_1:-48342198 Msa1197660:intron
AGGAATTCCCTTTCTTCTTT+TGG 0.234776 8_1:+48340631 None:intergenic
TGTATGTGTGTGTTGTTTAT+TGG 0.237468 8_1:+48343208 None:intergenic
AGTAAGTTATATGTAAGTTT+TGG 0.253096 8_1:-48340083 Msa1197660:three_prime_UTR
GAGCTGACTTGTTCTTCTTC+TGG 0.262316 8_1:+48340780 None:intergenic
TAGGGGAGTTCGACTCATTA+AGG 0.273487 8_1:+48340539 None:intergenic
CCTTGCACTGTTGATAATAT+AGG 0.282719 8_1:+48341599 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8_1:-48341665 Msa1197660:CDS
GATACTATAGTTTGGTAAAA+AGG 0.304774 8_1:-48340188 Msa1197660:three_prime_UTR
ATCTATATTAAAGGCGAAAT+CGG 0.315157 8_1:+48340382 None:intergenic
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8_1:-48341489 Msa1197660:CDS
GTATGTGTGTGTTGTTTATT+GGG 0.334443 8_1:+48343209 None:intergenic
TAGTGTTTGTTTGTGTATAT+AGG 0.343461 8_1:-48340261 Msa1197660:three_prime_UTR
GCCATCTTGAAGACTTTGAT+TGG 0.367238 8_1:+48343131 None:intergenic
AACTATGAATTCTAGATTGT+TGG 0.377091 8_1:-48340818 Msa1197660:intron
GTCATTACCATCTTTAACTT+AGG 0.380794 8_1:+48340129 None:intergenic
TTCATACCATGATGTGATTA+GGG 0.385029 8_1:-48341664 Msa1197660:CDS
GCACTCTTTACAAATATAGA+TGG 0.392130 8_1:-48340021 Msa1197660:three_prime_UTR
AGTGGTTGTTCAATCAAAAC+TGG 0.394347 8_1:+48340450 None:intergenic
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8_1:-48341630 Msa1197660:CDS
AGTATACTAGTCCTCCCATT+GGG 0.414569 8_1:+48340600 None:intergenic
TATTGTTTGGATTGTATGAA+TGG 0.420599 8_1:-48342251 Msa1197660:CDS
TGAGTGCAATATGTATTGTT+TGG 0.429253 8_1:-48342264 Msa1197660:CDS
TGGAAGACTCAAGAACATTA+AGG 0.435594 8_1:-48340705 Msa1197660:CDS
ATCTGTGAGATACTATAGTT+TGG 0.437513 8_1:-48340196 Msa1197660:three_prime_UTR
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8_1:-48342364 Msa1197660:CDS
CTTTGGACCTAAGTTAAAGA+TGG 0.456443 8_1:-48340136 Msa1197660:three_prime_UTR
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8_1:-48340725 Msa1197660:CDS
TCACTTAGTCGAGACATTAG+TGG 0.460633 8_1:+48340432 None:intergenic
AAGAAACAGTTAATAATCAT+AGG 0.470126 8_1:-48340577 Msa1197660:three_prime_UTR
TAATGTTCTTGAGTCTTCCA+TGG 0.472553 8_1:+48340708 None:intergenic
CATTCACGCAGTTGGAACAT+TGG 0.478234 8_1:-48340499 Msa1197660:three_prime_UTR
GCAAGGAATCTCTAACTGTA+GGG 0.483417 8_1:+48340521 None:intergenic
GGTGATCATCCGATCAAGAC+TGG 0.484391 8_1:-48340478 Msa1197660:three_prime_UTR
GAATAGCACGATGATCTTTG+TGG 0.487973 8_1:+48342201 None:intergenic
AGGGGAGTTCGACTCATTAA+GGG 0.493268 8_1:+48340540 None:intergenic
GATGGTAATGACAATAGTAT+AGG 0.493475 8_1:-48340118 Msa1197660:three_prime_UTR
TGCGGGATCCCTTGACTGAA+GGG 0.498524 8_1:-48340408 Msa1197660:three_prime_UTR
ATTCCTCATCGTGCCCCAAT+GGG 0.509643 8_1:-48340614 Msa1197660:CDS
ATTCCTTGCATTCACGCAGT+TGG 0.509800 8_1:-48340507 Msa1197660:three_prime_UTR
CAGATCGGCCAAAAGAAGAA+AGG 0.510241 8_1:-48340639 Msa1197660:CDS
CCTCAGCCAAGGCCCGGAAA+AGG 0.511324 8_1:-48341555 Msa1197660:CDS
AAGAAACGCTCTTTGAGAAG+AGG 0.515700 8_1:+48342323 None:intergenic
TTGTGATGAATGTGAGAAAG+TGG 0.518718 8_1:+48343178 None:intergenic
AACGACAGCACTTCCCGCAC+TGG 0.520737 8_1:+48340286 None:intergenic
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8_1:-48341481 Msa1197660:intron
CCTATATTATCAACAGTGCA+AGG 0.522886 8_1:-48341599 Msa1197660:CDS
GCCGATCTGTAATTGACTGT+TGG 0.527628 8_1:+48340653 None:intergenic
GAGAGGCCTCAGCCAAGGCC+CGG 0.529902 8_1:-48341561 Msa1197660:CDS
GTAATGACAATAGTATAGGT+TGG 0.530750 8_1:-48340114 Msa1197660:three_prime_UTR
AATTCCTCATCGTGCCCCAA+TGG 0.532814 8_1:-48340615 Msa1197660:CDS
AGATCGGCCAAAAGAAGAAA+GGG 0.533546 8_1:-48340638 Msa1197660:CDS
GTTCCAACTGCGTGAATGCA+AGG 0.534287 8_1:+48340504 None:intergenic
ATCGGATTCCCTTCAGTCAA+GGG 0.544915 8_1:+48340400 None:intergenic
TAGTATACTAGTCCTCCCAT+TGG 0.548247 8_1:+48340599 None:intergenic
CTGGTCTATCCAGTCTTGAT+CGG 0.548273 8_1:+48340469 None:intergenic
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8_1:+48341527 None:intergenic
TGCAAGGAATCTCTAACTGT+AGG 0.563310 8_1:+48340520 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.564886 8_1:+48340659 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8_1:+48341658 None:intergenic
AATCGGATTCCCTTCAGTCA+AGG 0.575945 8_1:+48340399 None:intergenic
GGGTTGTGTTCTTGAATGAG+AGG 0.577373 8_1:-48341578 Msa1197660:CDS
TCCAATCAAAGTCTTCAAGA+TGG 0.579850 8_1:-48343132 Msa1197660:exon
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8_1:-48341640 Msa1197660:CDS
TTGAGAAGAGGTTTCAACCA+TGG 0.588393 8_1:+48342335 None:intergenic
TTGGTGAAAAGGGCCAGTGC+GGG 0.588645 8_1:-48340299 Msa1197660:three_prime_UTR
TGTGATGAATGTGAGAAAGT+GGG 0.593199 8_1:+48343179 None:intergenic
GATCTGTAATTGACTGTTGG+TGG 0.598799 8_1:+48340656 None:intergenic
ATGCGGGATCCCTTGACTGA+AGG 0.599792 8_1:-48340409 Msa1197660:three_prime_UTR
ACTGTTGGTGGTGGTGTCGA+AGG 0.600407 8_1:+48340668 None:intergenic
TGTCTCGACTAAGTGAATGC+GGG 0.600588 8_1:-48340425 Msa1197660:three_prime_UTR
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8_1:-48342352 Msa1197660:CDS
CCTCATCGTGCCCCAATGGG+AGG 0.612946 8_1:-48340611 Msa1197660:CDS
ATGTCTCGACTAAGTGAATG+CGG 0.613597 8_1:-48340426 Msa1197660:three_prime_UTR
CCTCCCATTGGGGCACGATG+AGG 0.616154 8_1:+48340611 None:intergenic
TGAATGAGAGGCCTCAGCCA+AGG 0.624070 8_1:-48341566 Msa1197660:CDS
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8_1:+48341488 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8_1:+48341499 None:intergenic
CAAGGAATCTCTAACTGTAG+GGG 0.637324 8_1:+48340522 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8_1:-48342372 Msa1197660:CDS
ACCAACAGTCAATTACAGAT+CGG 0.643222 8_1:-48340654 Msa1197660:CDS
AGAAGAGGTTTCAACCATGG+TGG 0.644229 8_1:+48342338 None:intergenic
TTCAATGATATCAGACTCAG+AGG 0.648076 8_1:-48340753 Msa1197660:CDS
TTTGGTGAAAAGGGCCAGTG+CGG 0.648558 8_1:-48340300 Msa1197660:three_prime_UTR
TGTGTTTGTTACAGATAAGG+AGG 0.651544 8_1:-48341689 Msa1197660:intron
CTATATTATCAACAGTGCAA+GGG 0.657853 8_1:-48341598 Msa1197660:CDS
GTATACTAGTCCTCCCATTG+GGG 0.661515 8_1:+48340601 None:intergenic
CTGATGATGAGGAAAACAGG+TGG 0.717367 8_1:-48342361 Msa1197660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCAGTTTTATTTTTATTT+AGG - chr8_1:48340488-48340507 Msa1197660:three_prime_UTR 10.0%
!!! ATTTTAGAGAAAAAAAAAAT+TGG - chr8_1:48340877-48340896 Msa1197660:intron 10.0%
!!! TTTTAGAGAAAAAAAAAATT+GGG - chr8_1:48340878-48340897 Msa1197660:intron 10.0%
!!! AAATTTGATAATTTTTTTGA+AGG - chr8_1:48340953-48340972 Msa1197660:intron 10.0%
!!! AATTTGATAATTTTTTTGAA+GGG - chr8_1:48340954-48340973 Msa1197660:intron 10.0%
!!! ATTATTATTACTACTATTTT+GGG + chr8_1:48341953-48341972 None:intergenic 10.0%
!!! TAAACTAGTGTTAAATTTTT+TGG - chr8_1:48340395-48340414 Msa1197660:three_prime_UTR 15.0%
!!! TTATTTGTGTTTTTAAATTC+TGG - chr8_1:48340744-48340763 Msa1197660:CDS 15.0%
!!! TATTTGTGTTTTTAAATTCT+GGG - chr8_1:48340745-48340764 Msa1197660:CDS 15.0%
!! TAAAAATTAAAAACAGCAAA+AGG + chr8_1:48341323-48341342 None:intergenic 15.0%
!!! GATTATTATTACTACTATTT+TGG + chr8_1:48341954-48341973 None:intergenic 15.0%
!!! TTGATCAATATTTTAAATAG+TGG + chr8_1:48342251-48342270 None:intergenic 15.0%
!!! CTATTTAAAATATTGATCAA+TGG - chr8_1:48342251-48342270 Msa1197660:CDS 15.0%
!!! TTTTTTCGTATCTATATTAA+AGG + chr8_1:48342979-48342998 None:intergenic 15.0%
!!! ATGAACTAGTGTTAATTTTT+TGG - chr8_1:48340539-48340558 Msa1197660:three_prime_UTR 20.0%
!!! TGAACTAGTGTTAATTTTTT+GGG - chr8_1:48340540-48340559 Msa1197660:three_prime_UTR 20.0%
!!! TTAGTACTGTTTTTGAATTT+TGG - chr8_1:48340582-48340601 Msa1197660:exon 20.0%
!!! TAGTACTGTTTTTGAATTTT+GGG - chr8_1:48340583-48340602 Msa1197660:exon 20.0%
!!! CATTATTTTATGAGTTAACT+TGG - chr8_1:48340841-48340860 Msa1197660:intron 20.0%
!!! TTATTTGTGATTTTGAATTG+TGG - chr8_1:48340920-48340939 Msa1197660:intron 20.0%
!!! TATTTGTGATTTTGAATTGT+GGG - chr8_1:48340921-48340940 Msa1197660:intron 20.0%
!! AATACAATAAATGTGTCAAA+GGG + chr8_1:48341253-48341272 None:intergenic 20.0%
!! AACAATAAATGGGAATTTAT+GGG + chr8_1:48341359-48341378 None:intergenic 20.0%
!! AATAATTGAATTGATTGTAG+TGG + chr8_1:48341488-48341507 None:intergenic 20.0%
!!! ATTTGTTTTGATTGCAATTT+TGG - chr8_1:48342058-48342077 Msa1197660:intron 20.0%
!! TATTATAGACTATTCTTATG+AGG + chr8_1:48342137-48342156 None:intergenic 20.0%
!!! TCTTGTATTTTAGAGAATTA+CGG + chr8_1:48342172-48342191 None:intergenic 20.0%
!!! CAATATTTTAAATAGTGGAT+TGG + chr8_1:48342246-48342265 None:intergenic 20.0%
!! ATAATCATGAATGCTAATAT+AGG - chr8_1:48342344-48342363 Msa1197660:CDS 20.0%
!! ATGACAAAATTTCAAATGTT+GGG + chr8_1:48342401-48342420 None:intergenic 20.0%
!! AATGACAAAATTTCAAATGT+TGG + chr8_1:48342402-48342421 None:intergenic 20.0%
!! AATTGAATTGAATGCAAAAA+GGG + chr8_1:48342435-48342454 None:intergenic 20.0%
!! AAATTGAATTGAATGCAAAA+AGG + chr8_1:48342436-48342455 None:intergenic 20.0%
!! AAGAAACAGTTAATAATCAT+AGG - chr8_1:48342772-48342791 Msa1197660:intron 20.0%
!! TATAGATACGAAAAAATCTA+AGG - chr8_1:48342983-48343002 Msa1197660:intron 20.0%
!! AAATATGCAAAAAGTTTCTT+TGG - chr8_1:48343196-48343215 Msa1197660:five_prime_UTR 20.0%
!!! AGTAAGTTATATGTAAGTTT+TGG - chr8_1:48343266-48343285 Msa1197660:intron 20.0%
!!! GTAAGTTATATGTAAGTTTT+GGG - chr8_1:48343267-48343286 Msa1197660:intron 20.0%
!!! TAAGTTATATGTAAGTTTTG+GGG - chr8_1:48343268-48343287 Msa1197660:intron 20.0%
! AAGTTTATAACTATTTGAGG+CGG + chr8_1:48340065-48340084 None:intergenic 25.0%
! GTGAAGTTTATAACTATTTG+AGG + chr8_1:48340068-48340087 None:intergenic 25.0%
!!! TTGTTTGTGTTTTTGAATTC+TGG - chr8_1:48340438-48340457 Msa1197660:three_prime_UTR 25.0%
!!! TGTTTGTGTTTTTGAATTCT+GGG - chr8_1:48340439-48340458 Msa1197660:three_prime_UTR 25.0%
!! TTGTTTCGAATGTAATGTTT+TGG - chr8_1:48340717-48340736 Msa1197660:CDS 25.0%
!! TGTTTCGAATGTAATGTTTT+GGG - chr8_1:48340718-48340737 Msa1197660:CDS 25.0%
!! ATTTGTGATTTTGAATTGTG+GGG - chr8_1:48340922-48340941 Msa1197660:intron 25.0%
! TATTGTTTGGATTGTATGAA+TGG - chr8_1:48341098-48341117 Msa1197660:intron 25.0%
! GAATACAATAAATGTGTCAA+AGG + chr8_1:48341254-48341273 None:intergenic 25.0%
! GAACAATAAATGGGAATTTA+TGG + chr8_1:48341360-48341379 None:intergenic 25.0%
! TCTTATTCAAATGGTTAGAA+GGG + chr8_1:48341924-48341943 None:intergenic 25.0%
! ATCTTATTCAAATGGTTAGA+AGG + chr8_1:48341925-48341944 None:intergenic 25.0%
! CATCAAAATCCTTGATTTAA+CGG + chr8_1:48341994-48342013 None:intergenic 25.0%
! AAATTTCAAATGTTGGGTAT+AGG + chr8_1:48342395-48342414 None:intergenic 25.0%
!!! GAAATTTTGTCATTTCAGTA+TGG - chr8_1:48342408-48342427 Msa1197660:intron 25.0%
!!! TGTTTTGACTTTTGAATCTA+TGG - chr8_1:48342488-48342507 Msa1197660:intron 25.0%
! AACTATGAATTCTAGATTGT+TGG - chr8_1:48342531-48342550 Msa1197660:intron 25.0%
! ATCTATATTAAAGGCGAAAT+CGG + chr8_1:48342970-48342989 None:intergenic 25.0%
! TAGTGTTTGTTTGTGTATAT+AGG - chr8_1:48343088-48343107 Msa1197660:CDS 25.0%
! GATACTATAGTTTGGTAAAA+AGG - chr8_1:48343161-48343180 Msa1197660:five_prime_UTR 25.0%
! TATAGTTTGGTAAAAAGGAA+TGG - chr8_1:48343166-48343185 Msa1197660:five_prime_UTR 25.0%
!! TTTTTGTAGAACTTTGAAGG+CGG + chr8_1:48340026-48340045 None:intergenic 30.0%
! AGGTTTTTGTAGAACTTTGA+AGG + chr8_1:48340029-48340048 None:intergenic 30.0%
!! GTATGTGTGTGTTGTTTATT+GGG + chr8_1:48340143-48340162 None:intergenic 30.0%
!! TGTATGTGTGTGTTGTTTAT+TGG + chr8_1:48340144-48340163 None:intergenic 30.0%
!!! TGTTTTTGGGCTTAATTTCA+CGG + chr8_1:48340265-48340284 None:intergenic 30.0%
!! TTTTGGGAATGCAATGATTT+TGG - chr8_1:48340556-48340575 Msa1197660:three_prime_UTR 30.0%
! GTTTCGAATGTAATGTTTTG+GGG - chr8_1:48340719-48340738 Msa1197660:CDS 30.0%
!!! ATAATTTTTTTGAAGGGTGC+TGG - chr8_1:48340960-48340979 Msa1197660:intron 30.0%
TGAGTGCAATATGTATTGTT+TGG - chr8_1:48341085-48341104 Msa1197660:intron 30.0%
CACAACTTTGATGAATTCAA+TGG + chr8_1:48341194-48341213 None:intergenic 30.0%
! GTTTTTCTGACAACAAAAAC+AGG + chr8_1:48341276-48341295 None:intergenic 30.0%
!! AAAAACCATCACTTTTCCTT+TGG - chr8_1:48341290-48341309 Msa1197660:intron 30.0%
ATGGGAATTTATGGGATAAT+TGG + chr8_1:48341351-48341370 None:intergenic 30.0%
ATTATTGTCATTGTTGTGTC+TGG - chr8_1:48341540-48341559 Msa1197660:CDS 30.0%
! AGCTTTTCAATGCAATAACA+TGG - chr8_1:48341605-48341624 Msa1197660:CDS 30.0%
AACAACAGAGATCATTAGAA+AGG + chr8_1:48341636-48341655 None:intergenic 30.0%
TTCATACCATGATGTGATTA+GGG - chr8_1:48341685-48341704 Msa1197660:intron 30.0%
CTATATTATCAACAGTGCAA+GGG - chr8_1:48341751-48341770 Msa1197660:intron 30.0%
CTTATTCAAATGGTTAGAAG+GGG + chr8_1:48341923-48341942 None:intergenic 30.0%
GGGAGTTAATCTTATTCAAA+TGG + chr8_1:48341933-48341952 None:intergenic 30.0%
GTATATAGGAAAAATCCAAC+AGG - chr8_1:48343102-48343121 Msa1197660:CDS 30.0%
ATCTGTGAGATACTATAGTT+TGG - chr8_1:48343153-48343172 Msa1197660:five_prime_UTR 30.0%
! GTCATTACCATCTTTAACTT+AGG + chr8_1:48343223-48343242 None:intergenic 30.0%
! GATGGTAATGACAATAGTAT+AGG - chr8_1:48343231-48343250 Msa1197660:five_prime_UTR 30.0%
GTAATGACAATAGTATAGGT+TGG - chr8_1:48343235-48343254 Msa1197660:five_prime_UTR 30.0%
GCACTCTTTACAAATATAGA+TGG - chr8_1:48343328-48343347 Msa1197660:intron 30.0%
TGTGATGAATGTGAGAAAGT+GGG + chr8_1:48340173-48340192 None:intergenic 35.0%
TTGTGATGAATGTGAGAAAG+TGG + chr8_1:48340174-48340193 None:intergenic 35.0%
TCCAATCAAAGTCTTCAAGA+TGG - chr8_1:48340217-48340236 Msa1197660:three_prime_UTR 35.0%
GTGAAATTAAGCCCAAAAAC+AGG - chr8_1:48340264-48340283 Msa1197660:three_prime_UTR 35.0%
!! ACCATGATTCTCCTGTTTTT+GGG + chr8_1:48340278-48340297 None:intergenic 35.0%
! TACCATGATTCTCCTGTTTT+TGG + chr8_1:48340279-48340298 None:intergenic 35.0%
TGAACACACAATGAGATTCT+TGG + chr8_1:48340656-48340675 None:intergenic 35.0%
CAAATCCAAAGGAAAAGTGA+TGG + chr8_1:48341298-48341317 None:intergenic 35.0%
!! CATCACTTTTCCTTTGGATT+TGG - chr8_1:48341296-48341315 Msa1197660:intron 35.0%
GGTGGTTACGAACAATAAAT+GGG + chr8_1:48341369-48341388 None:intergenic 35.0%
TGGTGGTTACGAACAATAAA+TGG + chr8_1:48341370-48341389 None:intergenic 35.0%
TTGACACCAACATCAAATGT+CGG + chr8_1:48341452-48341471 None:intergenic 35.0%
! GTGTGTGTTTGTTACAGATA+AGG - chr8_1:48341657-48341676 Msa1197660:CDS 35.0%
! TGTGTTTGTTACAGATAAGG+AGG - chr8_1:48341660-48341679 Msa1197660:CDS 35.0%
CTTCATACCATGATGTGATT+AGG - chr8_1:48341684-48341703 Msa1197660:intron 35.0%
! GAATGAGATTCAGAAAGTGT+TGG - chr8_1:48341709-48341728 Msa1197660:intron 35.0%
!! CCTTGCACTGTTGATAATAT+AGG + chr8_1:48341753-48341772 None:intergenic 35.0%
CCTATATTATCAACAGTGCA+AGG - chr8_1:48341750-48341769 Msa1197660:intron 35.0%
! ATAATCCGCTACCAAACTTT+TGG - chr8_1:48341968-48341987 Msa1197660:intron 35.0%
TGATTTAACGGCCAAAAGTT+TGG + chr8_1:48341982-48342001 None:intergenic 35.0%
! AACTTTTGGCCGTTAAATCA+AGG - chr8_1:48341982-48342001 Msa1197660:intron 35.0%
ACCTCAATGTTAATGCATTG+AGG - chr8_1:48342092-48342111 Msa1197660:intron 35.0%
ACCTCAATGCATTAACATTG+AGG + chr8_1:48342096-48342115 None:intergenic 35.0%
TTTCAAGCTAGCACTTAAAG+TGG - chr8_1:48342308-48342327 Msa1197660:CDS 35.0%
TTCAAGCTAGCACTTAAAGT+GGG - chr8_1:48342309-48342328 Msa1197660:CDS 35.0%
! GACTTTTGAATCTATGGCAT+TGG - chr8_1:48342494-48342513 Msa1197660:intron 35.0%
TTCAATGATATCAGACTCAG+AGG - chr8_1:48342596-48342615 Msa1197660:intron 35.0%
TAATGTTCTTGAGTCTTCCA+TGG + chr8_1:48342644-48342663 None:intergenic 35.0%
TGGAAGACTCAAGAACATTA+AGG - chr8_1:48342644-48342663 Msa1197660:intron 35.0%
ACCAACAGTCAATTACAGAT+CGG - chr8_1:48342695-48342714 Msa1197660:intron 35.0%
! AGGAATTCCCTTTCTTCTTT+TGG + chr8_1:48342721-48342740 None:intergenic 35.0%
AGTGGTTGTTCAATCAAAAC+TGG + chr8_1:48342902-48342921 None:intergenic 35.0%
!! CTAGAGCTTTTTGGTGAAAA+GGG - chr8_1:48343040-48343059 Msa1197660:intron 35.0%
TTGGTAAAAAGGAATGGTGT+AGG - chr8_1:48343172-48343191 Msa1197660:five_prime_UTR 35.0%
CTTTGGACCTAAGTTAAAGA+TGG - chr8_1:48343213-48343232 Msa1197660:five_prime_UTR 35.0%
!!! AGTTTTGGGGATGTAAATAG+TGG - chr8_1:48343281-48343300 Msa1197660:intron 35.0%
GCCATCTTGAAGACTTTGAT+TGG + chr8_1:48340221-48340240 None:intergenic 40.0%
!!! ATTTTTTTGAAGGGTGCTGG+AGG - chr8_1:48340963-48340982 Msa1197660:intron 40.0%
TTGAGAAGAGGTTTCAACCA+TGG + chr8_1:48341017-48341036 None:intergenic 40.0%
! AAGAAACGCTCTTTGAGAAG+AGG + chr8_1:48341029-48341048 None:intergenic 40.0%
GAATAGCACGATGATCTTTG+TGG + chr8_1:48341151-48341170 None:intergenic 40.0%
! CAAAGATCATCGTGCTATTC+AGG - chr8_1:48341151-48341170 Msa1197660:intron 40.0%
AGCAAAAGGACCAAATCCAA+AGG + chr8_1:48341309-48341328 None:intergenic 40.0%
!! TTGATGTTGGTGTCAAATGC+CGG - chr8_1:48341456-48341475 Msa1197660:intron 40.0%
CATTCACCCTAATCACATCA+TGG + chr8_1:48341694-48341713 None:intergenic 40.0%
!! CAGAAAGTGTTGGACATAAC+AGG - chr8_1:48341719-48341738 Msa1197660:intron 40.0%
AGAATCGAAAGGAATCGAGA+AGG + chr8_1:48341853-48341872 None:intergenic 40.0%
TCCTTTCGATTCTGTTCTCT+TGG - chr8_1:48341860-48341879 Msa1197660:intron 40.0%
AAGGGGAAGACATTATGTGA+AGG + chr8_1:48341906-48341925 None:intergenic 40.0%
GATCTGTAATTGACTGTTGG+TGG + chr8_1:48342696-48342715 None:intergenic 40.0%
AGATCGGCCAAAAGAAGAAA+GGG - chr8_1:48342711-48342730 Msa1197660:intron 40.0%
AGTATACTAGTCCTCCCATT+GGG + chr8_1:48342752-48342771 None:intergenic 40.0%
TAGTATACTAGTCCTCCCAT+TGG + chr8_1:48342753-48342772 None:intergenic 40.0%
CAAGGAATCTCTAACTGTAG+GGG + chr8_1:48342830-48342849 None:intergenic 40.0%
GCAAGGAATCTCTAACTGTA+GGG + chr8_1:48342831-48342850 None:intergenic 40.0%
TGCAAGGAATCTCTAACTGT+AGG + chr8_1:48342832-48342851 None:intergenic 40.0%
TCACTTAGTCGAGACATTAG+TGG + chr8_1:48342920-48342939 None:intergenic 40.0%
ATGTCTCGACTAAGTGAATG+CGG - chr8_1:48342923-48342942 Msa1197660:intron 40.0%
!! GCTAGAGCTTTTTGGTGAAA+AGG - chr8_1:48343039-48343058 Msa1197660:intron 40.0%
TAGGAAAAATCCAACAGGAG+TGG - chr8_1:48343107-48343126 Msa1197660:CDS 40.0%
!! CTAACTTTTTCCACTCCTGT+TGG + chr8_1:48343120-48343139 None:intergenic 40.0%
CAACAGGAGTGGAAAAAGTT+AGG - chr8_1:48343118-48343137 Msa1197660:exon 40.0%
! CTATTTGAGGCGGTGAAAGA+GGG + chr8_1:48340055-48340074 None:intergenic 45.0%
! ACTATTTGAGGCGGTGAAAG+AGG + chr8_1:48340056-48340075 None:intergenic 45.0%
GCCCAAAAACAGGAGAATCA+TGG - chr8_1:48340274-48340293 Msa1197660:three_prime_UTR 45.0%
AGCTCACACACTCTCATTGT+TGG - chr8_1:48340347-48340366 Msa1197660:three_prime_UTR 45.0%
! GTCCTGATGATGAGGAAAAC+AGG - chr8_1:48340985-48341004 Msa1197660:intron 45.0%
! CACCTGTTTTCCTCATCATC+AGG + chr8_1:48340990-48341009 None:intergenic 45.0%
! CTGATGATGAGGAAAACAGG+TGG - chr8_1:48340988-48341007 Msa1197660:intron 45.0%
AGAAGAGGTTTCAACCATGG+TGG + chr8_1:48341014-48341033 None:intergenic 45.0%
CAATCTAAACCATCGATGCG+TGG + chr8_1:48341390-48341409 None:intergenic 45.0%
TGTCTGACACCAACATGTGT+CGG + chr8_1:48341429-48341448 None:intergenic 45.0%
CAGACACCGACATTTGATGT+TGG - chr8_1:48341443-48341462 Msa1197660:intron 45.0%
TTGATTGTAGTGGCAGACAC+CGG + chr8_1:48341478-48341497 None:intergenic 45.0%
ATGCGACACTGAACTAGACA+CGG + chr8_1:48341569-48341588 None:intergenic 45.0%
GGGTTGTGTTCTTGAATGAG+AGG - chr8_1:48341771-48341790 Msa1197660:intron 45.0%
!!! AGTGTTGGTGACACCTTTTC+CGG + chr8_1:48341810-48341829 None:intergenic 45.0%
GCCAAGAGAACAGAATCGAA+AGG + chr8_1:48341864-48341883 None:intergenic 45.0%
ATTCTGTTCTCTTGGCTGCA+AGG - chr8_1:48341868-48341887 Msa1197660:intron 45.0%
AACGGCCAAAAGTTTGGTAG+CGG + chr8_1:48341976-48341995 None:intergenic 45.0%
!! GAGCTGACTTGTTCTTCTTC+TGG + chr8_1:48342572-48342591 None:intergenic 45.0%
! CTGTAATTGACTGTTGGTGG+TGG + chr8_1:48342693-48342712 None:intergenic 45.0%
GCCGATCTGTAATTGACTGT+TGG + chr8_1:48342699-48342718 None:intergenic 45.0%
CAGATCGGCCAAAAGAAGAA+AGG - chr8_1:48342710-48342729 Msa1197660:intron 45.0%
GTATACTAGTCCTCCCATTG+GGG + chr8_1:48342751-48342770 None:intergenic 45.0%
AGGGGAGTTCGACTCATTAA+GGG + chr8_1:48342812-48342831 None:intergenic 45.0%
TAGGGGAGTTCGACTCATTA+AGG + chr8_1:48342813-48342832 None:intergenic 45.0%
ATTCCTTGCATTCACGCAGT+TGG - chr8_1:48342842-48342861 Msa1197660:intron 45.0%
CATTCACGCAGTTGGAACAT+TGG - chr8_1:48342850-48342869 Msa1197660:intron 45.0%
CTGGTCTATCCAGTCTTGAT+CGG + chr8_1:48342883-48342902 None:intergenic 45.0%
TGTCTCGACTAAGTGAATGC+GGG - chr8_1:48342924-48342943 Msa1197660:intron 45.0%
ATCGGATTCCCTTCAGTCAA+GGG + chr8_1:48342952-48342971 None:intergenic 45.0%
AATCGGATTCCCTTCAGTCA+AGG + chr8_1:48342953-48342972 None:intergenic 45.0%
! GTTCCAGTGCTAGAGCTTTT+TGG - chr8_1:48343031-48343050 Msa1197660:intron 45.0%
! TCACCAAAAAGCTCTAGCAC+TGG + chr8_1:48343037-48343056 None:intergenic 45.0%
TCTAAACCATCGATGCGTGG+TGG + chr8_1:48341387-48341406 None:intergenic 50.0%
ACACCAACATGTGTCGGACA+GGG + chr8_1:48341423-48341442 None:intergenic 50.0%
!!! GTGTTGGTGACACCTTTTCC+GGG + chr8_1:48341809-48341828 None:intergenic 50.0%
CTCGCAGACTTCACAAGTGT+TGG + chr8_1:48341825-48341844 None:intergenic 50.0%
AATTCCTCATCGTGCCCCAA+TGG - chr8_1:48342734-48342753 Msa1197660:intron 50.0%
ATTCCTCATCGTGCCCCAAT+GGG - chr8_1:48342735-48342754 Msa1197660:intron 50.0%
GTTCCAACTGCGTGAATGCA+AGG + chr8_1:48342848-48342867 None:intergenic 50.0%
GGTGATCATCCGATCAAGAC+TGG - chr8_1:48342871-48342890 Msa1197660:intron 50.0%
TTTGGTGAAAAGGGCCAGTG+CGG - chr8_1:48343049-48343068 Msa1197660:intron 50.0%
GAGGCGGTGAAAGAGGGAAT+AGG + chr8_1:48340049-48340068 None:intergenic 55.0%
! TGCTGGAGGTCCTGATGATG+AGG - chr8_1:48340977-48340996 Msa1197660:intron 55.0%
AGGAAAACAGGTGGCCACCA+TGG - chr8_1:48340997-48341016 Msa1197660:intron 55.0%
TCGTAACCACCACGCATCGA+TGG - chr8_1:48341378-48341397 Msa1197660:intron 55.0%
GTTCCCTGTCCGACACATGT+TGG - chr8_1:48341417-48341436 Msa1197660:intron 55.0%
GACACCAACATGTGTCGGAC+AGG + chr8_1:48341424-48341443 None:intergenic 55.0%
TGAATGAGAGGCCTCAGCCA+AGG - chr8_1:48341783-48341802 Msa1197660:intron 55.0%
!! ACTGTTGGTGGTGGTGTCGA+AGG + chr8_1:48342684-48342703 None:intergenic 55.0%
ATGCGGGATCCCTTGACTGA+AGG - chr8_1:48342940-48342959 Msa1197660:intron 55.0%
TGCGGGATCCCTTGACTGAA+GGG - chr8_1:48342941-48342960 Msa1197660:intron 55.0%
TTGGTGAAAAGGGCCAGTGC+GGG - chr8_1:48343050-48343069 Msa1197660:intron 55.0%
! GTGACACCTTTTCCGGGCCT+TGG + chr8_1:48341803-48341822 None:intergenic 60.0%
CACAGCAGCAGTTGCAGCCA+TGG - chr8_1:48342624-48342643 Msa1197660:intron 60.0%
AACGACAGCACTTCCCGCAC+TGG + chr8_1:48343066-48343085 None:intergenic 60.0%
! CCTTTTCCGGGCCTTGGCTG+AGG + chr8_1:48341797-48341816 None:intergenic 65.0%
CCTCAGCCAAGGCCCGGAAA+AGG - chr8_1:48341794-48341813 Msa1197660:intron 65.0%
CCTCCCATTGGGGCACGATG+AGG + chr8_1:48342741-48342760 None:intergenic 65.0%
CCTCATCGTGCCCCAATGGG+AGG - chr8_1:48342738-48342757 Msa1197660:intron 65.0%
GAGAGGCCTCAGCCAAGGCC+CGG - chr8_1:48341788-48341807 Msa1197660:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 48339965 48343406 48339965 ID=Msa1197660;Name=Msa1197660
chr8_1 mRNA 48339965 48343406 48339965 ID=Msa1197660-mRNA-1;Parent=Msa1197660;Name=Msa1197660-mRNA-1;_AED=0.05;_eAED=0.05;_QI=168|1|1|1|0.75|0.6|5|633|233
chr8_1 exon 48339965 48340825 48339965 ID=Msa1197660-mRNA-1:exon:15285;Parent=Msa1197660-mRNA-1
chr8_1 exon 48341482 48341697 48341482 ID=Msa1197660-mRNA-1:exon:15284;Parent=Msa1197660-mRNA-1
chr8_1 exon 48342199 48342396 48342199 ID=Msa1197660-mRNA-1:exon:15283;Parent=Msa1197660-mRNA-1
chr8_1 exon 48343076 48343283 48343076 ID=Msa1197660-mRNA-1:exon:15282;Parent=Msa1197660-mRNA-1
chr8_1 exon 48343387 48343406 48343387 ID=Msa1197660-mRNA-1:exon:15281;Parent=Msa1197660-mRNA-1
chr8_1 five_prime_UTR 48343387 48343406 48343387 ID=Msa1197660-mRNA-1:five_prime_utr;Parent=Msa1197660-mRNA-1
chr8_1 five_prime_UTR 48343136 48343283 48343136 ID=Msa1197660-mRNA-1:five_prime_utr;Parent=Msa1197660-mRNA-1
chr8_1 CDS 48343076 48343135 48343076 ID=Msa1197660-mRNA-1:cds;Parent=Msa1197660-mRNA-1
chr8_1 CDS 48342199 48342396 48342199 ID=Msa1197660-mRNA-1:cds;Parent=Msa1197660-mRNA-1
chr8_1 CDS 48341482 48341697 48341482 ID=Msa1197660-mRNA-1:cds;Parent=Msa1197660-mRNA-1
chr8_1 CDS 48340598 48340825 48340598 ID=Msa1197660-mRNA-1:cds;Parent=Msa1197660-mRNA-1
chr8_1 three_prime_UTR 48339965 48340597 48339965 ID=Msa1197660-mRNA-1:three_prime_utr;Parent=Msa1197660-mRNA-1
Gene Sequence

>Msa1197660

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTTCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGTGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGATAAGGAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAGAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGATTGTTGGAACTTCAATGAATTTCCAGAAGAAGAACAAGTCAGCTCGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTCAAGAACATTAAGGTGCAAAGTTTCACTCCTTCGACACCACCACCAACAGTCAATTACAGATCGGCCAAAAGAAGAAAGGGAATTCCTCATCGTGCCCCAATGGGAGGACTAGTATACTAA
Protein sequence

>Msa1197660

MAFENQDTVREIKPKNRRIMGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSMNFQKKNKSARASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVY*