AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1291990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291990 MtrunA17_Chr8g0366581 100.000 232 0 0 1 232 1 232 1.28e-177 486
Msa1291990 MtrunA17_Chr5g0411151 85.897 234 29 3 1 231 1 233 1.04e-147 411
Msa1291990 MtrunA17_Chr5g0395181 70.370 216 54 4 19 233 7 213 3.45e-108 310
Msa1291990 MtrunA17_Chr4g0040071 72.414 203 52 4 31 232 13 212 5.92e-104 299
Msa1291990 MtrunA17_Chr1g0179781 55.340 206 73 5 33 229 7 202 3.22e-73 221
Msa1291990 MtrunA17_Chr7g0265291 58.333 204 71 5 33 229 7 203 1.99e-71 216
Msa1291990 MtrunA17_Chr2g0308401 49.265 136 59 4 34 159 5 140 5.70e-39 132
Msa1291990 MtrunA17_Chr4g0020811 47.794 136 61 3 34 159 7 142 8.01e-39 132
Msa1291990 MtrunA17_Chr4g0065041 48.031 127 65 1 33 159 8 133 4.69e-37 130
Msa1291990 MtrunA17_Chr2g0297251 34.685 222 118 5 33 228 37 257 6.25e-37 130
Msa1291990 MtrunA17_Chr2g0320521 34.052 232 109 6 34 228 7 231 2.09e-36 128
Msa1291990 MtrunA17_Chr7g0230511 32.922 243 117 5 30 228 6 246 2.49e-36 128
Msa1291990 MtrunA17_Chr2g0320561 46.715 137 63 4 34 160 5 141 6.36e-32 117
Msa1291990 MtrunA17_Chr2g0320551 43.382 136 67 3 34 159 5 140 4.33e-31 112
Msa1291990 MtrunA17_Chr4g0052451 43.902 82 40 1 85 160 11 92 3.33e-20 82.0
Msa1291990 MtrunA17_Chr8g0392151 33.951 162 82 6 19 159 1 158 1.17e-16 76.3
Msa1291990 MtrunA17_Chr8g0386511 29.193 161 99 4 72 228 4 153 1.41e-15 71.6
Msa1291990 MtrunA17_Chr5g0434331 27.670 206 105 9 29 228 39 206 1.17e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291990 AT4G17900.1 73.391 233 52 5 1 229 1 227 2.72e-122 347
Msa1291990 AT4G17900.2 72.906 203 46 4 30 229 17 213 6.39e-104 300
Msa1291990 AT1G32700.1 71.495 214 51 5 24 232 2 210 7.31e-102 294
Msa1291990 AT1G32700.2 71.023 176 42 4 61 232 1 171 2.53e-78 233
Msa1291990 AT5G46710.1 52.521 238 90 8 1 228 1 225 6.94e-78 234
Msa1291990 AT1G21000.2 50.209 239 82 4 26 228 6 243 6.90e-77 232
Msa1291990 AT1G21000.1 50.209 239 82 4 26 228 9 246 8.38e-77 232
Msa1291990 AT1G76590.1 50.877 228 80 4 33 228 18 245 2.11e-74 226
Msa1291990 AT5G46710.3 52.830 212 84 6 26 228 5 209 3.23e-72 219
Msa1291990 AT5G46710.2 59.627 161 57 3 1 159 1 155 1.71e-66 203
Msa1291990 AT1G43000.2 46.083 217 101 4 26 228 9 223 2.64e-62 194
Msa1291990 AT1G43000.1 46.083 217 101 4 26 228 2 216 3.53e-62 194
Msa1291990 AT2G27930.1 44.712 208 83 5 33 230 4 189 3.93e-53 170
Msa1291990 AT2G27930.4 44.712 208 83 5 33 230 4 189 3.93e-53 170
Msa1291990 AT2G27930.3 55.474 137 51 2 33 159 4 140 8.81e-49 158
Msa1291990 AT2G27930.2 55.474 137 51 2 33 159 4 140 8.81e-49 158
Msa1291990 AT2G12646.3 48.819 127 64 1 33 159 3 128 2.65e-37 131
Msa1291990 AT2G12646.1 48.819 127 64 1 33 159 6 131 2.82e-37 131
Msa1291990 AT2G12646.2 48.819 127 64 1 33 159 6 131 2.82e-37 131
Msa1291990 AT3G60670.1 37.949 195 105 3 28 221 2 181 2.09e-36 129
Msa1291990 AT1G31040.1 34.513 226 116 5 33 228 19 242 2.23e-34 123
Msa1291990 AT2G27930.5 45.455 154 53 4 86 230 61 192 5.89e-34 120
Msa1291990 AT2G27930.6 45.455 154 53 4 86 230 61 192 5.89e-34 120
Msa1291990 AT2G01818.1 34.579 214 115 9 35 228 13 221 3.75e-22 91.3
Msa1291990 AT2G01818.2 33.987 153 81 7 92 228 9 157 9.85e-11 58.9

Find 92 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAATTCCCTTTCTTCTTT+TGG 0.193854 8_3:+47642626 None:intergenic
CAAAGATCATCGTGCTATTC+AGG 0.214126 8_3:-47644141 Msa1291990:intron
TGTATGTGTGTGTTGTTTAT+TGG 0.237468 8_3:+47645151 None:intergenic
AGTAAGTTATATGTAAGTTT+TGG 0.253096 8_3:-47642079 Msa1291990:three_prime_UTR
GGGCTGACTTGTTCTTCTTC+TGG 0.253596 8_3:+47642775 None:intergenic
TAGGGGAGTTCGACTCATTA+AGG 0.273487 8_3:+47642534 None:intergenic
CCTTGCACTGTTGATAATAT+AGG 0.282719 8_3:+47643577 None:intergenic
CTTCATACCATGATGTGATT+AGG 0.304121 8_3:-47643643 Msa1291990:CDS
GATACTATAGTTTGGTAAAA+AGG 0.304774 8_3:-47642184 Msa1291990:three_prime_UTR
AGTGGTTGTTCGATCAAAAT+TGG 0.313432 8_3:+47642445 None:intergenic
TCCTTTCGATTCTGTTCTCT+TGG 0.334164 8_3:-47643467 Msa1291990:CDS
GTATGTGTGTGTTGTTTATT+GGG 0.334443 8_3:+47645152 None:intergenic
TAGTGTTTGTTTGTGTATAT+AGG 0.343461 8_3:-47642256 Msa1291990:three_prime_UTR
GCCATCTTGAAGACTTTGAT+TGG 0.367238 8_3:+47645074 None:intergenic
AACTATGAATTCTAGATTGT+TGG 0.377091 8_3:-47642813 Msa1291990:intron
GTCATTACCATCTTTAACTT+AGG 0.380794 8_3:+47642125 None:intergenic
TTCATACCATGATGTGATTA+GGG 0.385029 8_3:-47643642 Msa1291990:CDS
GCACTCTTTACAAATATAGA+TGG 0.392130 8_3:-47642017 Msa1291990:three_prime_UTR
CAGAAAGTGTTGGACATAAC+AGG 0.395592 8_3:-47643608 Msa1291990:CDS
ACTGTTGGTGGTGGTGTTGA+AGG 0.411165 8_3:+47642663 None:intergenic
AGTATACTAGTCCTCCCATT+GGG 0.414569 8_3:+47642595 None:intergenic
TATTGTTTGGATTGTATGAA+TGG 0.420599 8_3:-47644194 Msa1291990:CDS
TGAGTGCAATATGTATTGTT+TGG 0.429253 8_3:-47644207 Msa1291990:CDS
TGGAAGACTGAAGAACATTA+AGG 0.432710 8_3:-47642700 Msa1291990:CDS
ATCTGTGAGATACTATAGTT+TGG 0.437513 8_3:-47642192 Msa1291990:three_prime_UTR
GTCCTGATGATGAGGAAAAC+AGG 0.450549 8_3:-47644307 Msa1291990:CDS
CTTTGGACCTAAGTTAAAGA+TGG 0.456443 8_3:-47642132 Msa1291990:three_prime_UTR
CACAGCAGCAGTTGCAGCCA+TGG 0.460151 8_3:-47642720 Msa1291990:CDS
TCACTTAGTCGAGACATTAG+TGG 0.460633 8_3:+47642427 None:intergenic
TAATGTTCTTCAGTCTTCCA+TGG 0.466799 8_3:+47642703 None:intergenic
CAGATCAGCCAAAAGAAGAA+AGG 0.467780 8_3:-47642634 Msa1291990:CDS
TGAGGCGGTGAAAGAGGGAA+TGG 0.468772 8_3:+47645251 None:intergenic
AAGAAACAGTTAATAATCAT+AGG 0.470126 8_3:-47642572 Msa1291990:three_prime_UTR
CATTCACGCAGTTGGAACAT+TGG 0.478234 8_3:-47642494 Msa1291990:three_prime_UTR
GGTGATCATCCGATCAAGAC+TGG 0.484391 8_3:-47642473 Msa1291990:three_prime_UTR
GTGAAGTTTATAACTATTTG+AGG 0.486326 8_3:+47645233 None:intergenic
GAATAGCACGATGATCTTTG+TGG 0.487973 8_3:+47644144 None:intergenic
GCTGATCTGTAATTGACTGT+TGG 0.490334 8_3:+47642648 None:intergenic
GAGGCGGTGAAAGAGGGAAT+GGG 0.492160 8_3:+47645252 None:intergenic
AGGGGAGTTCGACTCATTAA+GGG 0.493268 8_3:+47642535 None:intergenic
GATGGTAATGACAATAGTAT+AGG 0.493475 8_3:-47642114 Msa1291990:three_prime_UTR
ATTTCTTGCATTCACGCAGT+TGG 0.495449 8_3:-47642502 Msa1291990:three_prime_UTR
GCAAGAAATCTCTAACTGTA+GGG 0.495561 8_3:+47642516 None:intergenic
TGCGGGATCCCTTGACTGAA+GGG 0.498524 8_3:-47642403 Msa1291990:three_prime_UTR
AGATCAGCCAAAAGAAGAAA+GGG 0.506864 8_3:-47642633 Msa1291990:CDS
ATTCCTCATCGTGCCCCAAT+GGG 0.509643 8_3:-47642609 Msa1291990:CDS
CCTCAGCCAAGGCCCGGAAA+AGG 0.511324 8_3:-47643533 Msa1291990:CDS
AACGACAGCACTTCCAGCAC+TGG 0.512333 8_3:+47642281 None:intergenic
AAGAAACGCTCTTTGAGAAG+AGG 0.515700 8_3:+47644266 None:intergenic
TTGTGATGAATGTGAGAAAG+TGG 0.518718 8_3:+47645121 None:intergenic
TGTGTTTGATATAGATAAGG+AGG 0.521409 8_3:-47643667 Msa1291990:intron
ATTCTGTTCTCTTGGCTGCA+AGG 0.521419 8_3:-47643459 Msa1291990:intron
CCTATATTATCAACAGTGCA+AGG 0.522886 8_3:-47643577 Msa1291990:CDS
GTCTGATATCATTGAAGCAC+GGG 0.526427 8_3:+47642755 None:intergenic
ACTATTTGAGGCGGTGAAAG+AGG 0.528231 8_3:+47645245 None:intergenic
GAGAGGCCTCAGCCAAGGCC+CGG 0.529902 8_3:-47643539 Msa1291990:CDS
GTAATGACAATAGTATAGGT+TGG 0.530750 8_3:-47642110 Msa1291990:three_prime_UTR
TTGGTGAAAAGGGCCAGTGC+TGG 0.531994 8_3:-47642294 Msa1291990:three_prime_UTR
AATTCCTCATCGTGCCCCAA+TGG 0.532814 8_3:-47642610 Msa1291990:CDS
GATCTGTAATTGACTGTTGG+TGG 0.533120 8_3:+47642651 None:intergenic
CTGTAATTGACTGTTGGTGG+TGG 0.534739 8_3:+47642654 None:intergenic
TGCAAGAAATCTCTAACTGT+AGG 0.539096 8_3:+47642515 None:intergenic
ATCAGATTCCCTTCAGTCAA+GGG 0.544915 8_3:+47642395 None:intergenic
TAGTATACTAGTCCTCCCAT+TGG 0.548247 8_3:+47642594 None:intergenic
CTCGCAGACTTCACAAGTGT+TGG 0.560178 8_3:+47643505 None:intergenic
CATTCACCCTAATCACATCA+TGG 0.571167 8_3:+47643636 None:intergenic
AATCAGATTCCCTTCAGTCA+AGG 0.573649 8_3:+47642394 None:intergenic
TTGGTCTATCCAGTCTTGAT+CGG 0.575600 8_3:+47642464 None:intergenic
GGGTTGTGTTCTTGAATGAG+AGG 0.577373 8_3:-47643556 Msa1291990:CDS
CTATTTGAGGCGGTGAAAGA+GGG 0.577524 8_3:+47645246 None:intergenic
TCCAATCAAAGTCTTCAAGA+TGG 0.579850 8_3:-47645075 Msa1291990:exon
GAATGAGATTCAGAAAGTGT+TGG 0.585916 8_3:-47643618 Msa1291990:CDS
TTGAGAAGAGGTTTCAACCA+TGG 0.588393 8_3:+47644278 None:intergenic
TGTGATGAATGTGAGAAAGT+GGG 0.593199 8_3:+47645122 None:intergenic
ATGCGGGATCCCTTGACTGA+AGG 0.599792 8_3:-47642404 Msa1291990:three_prime_UTR
TGTCTCGACTAAGTGAATGC+GGG 0.600588 8_3:-47642420 Msa1291990:three_prime_UTR
AGGAAAACAGGTGGCCACCA+TGG 0.603560 8_3:-47644295 Msa1291990:CDS
AGTCTGATATCATTGAAGCA+CGG 0.605702 8_3:+47642754 None:intergenic
CCTCATCGTGCCCCAATGGG+AGG 0.612946 8_3:-47642606 Msa1291990:CDS
CAAGAAATCTCTAACTGTAG+GGG 0.613111 8_3:+47642517 None:intergenic
ATGTCTCGACTAAGTGAATG+CGG 0.613597 8_3:-47642421 Msa1291990:three_prime_UTR
CCTCCCATTGGGGCACGATG+AGG 0.616154 8_3:+47642606 None:intergenic
TGAATGAGAGGCCTCAGCCA+AGG 0.624070 8_3:-47643544 Msa1291990:CDS
GCCAAGAGAACAGAATCGAA+AGG 0.631094 8_3:+47643466 None:intergenic
AGAATCGAAAGGAATCGAGA+AGG 0.635045 8_3:+47643477 None:intergenic
TGCTGGAGGTCCTGATGATG+AGG 0.642425 8_3:-47644315 Msa1291990:CDS
AGAAGAGGTTTCAACCATGG+TGG 0.644229 8_3:+47644281 None:intergenic
TTCAATGATATCAGACTCAG+AGG 0.648076 8_3:-47642748 Msa1291990:CDS
CTATATTATCAACAGTGCAA+GGG 0.657853 8_3:-47643576 Msa1291990:CDS
GTATACTAGTCCTCCCATTG+GGG 0.661515 8_3:+47642596 None:intergenic
AAGTTTATAACTATTTGAGG+CGG 0.677406 8_3:+47645236 None:intergenic
CTGATGATGAGGAAAACAGG+TGG 0.717367 8_3:-47644304 Msa1291990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAGAGAAAAAAAAAT+TGG - chr8_3:47642827-47642846 Msa1291990:intron 10.0%
!!! ATTTTAGAGAAAAAAAAATT+GGG - chr8_3:47642828-47642847 Msa1291990:intron 10.0%
!!! AAATTTGATAATTTTTTTGA+AGG - chr8_3:47642903-47642922 Msa1291990:intron 10.0%
!!! AATTTGATAATTTTTTTGAA+GGG - chr8_3:47642904-47642923 Msa1291990:intron 10.0%
!!! ATTATTATTACTACTATTTT+GGG + chr8_3:47643868-47643887 None:intergenic 10.0%
!!! TAAACTAGTGTTAATTTTTT+TGG - chr8_3:47642339-47642358 Msa1291990:three_prime_UTR 15.0%
!!! TTATTTGTGTTTTTAAATTC+TGG - chr8_3:47642695-47642714 Msa1291990:CDS 15.0%
!!! TATTTGTGTTTTTAAATTCT+GGG - chr8_3:47642696-47642715 Msa1291990:CDS 15.0%
!!! GATTATTATTACTACTATTT+TGG + chr8_3:47643869-47643888 None:intergenic 15.0%
!!! TTGATCAATATTTTAAATAG+TGG + chr8_3:47644150-47644169 None:intergenic 15.0%
!!! CTATTTAAAATATTGATCAA+TGG - chr8_3:47644150-47644169 Msa1291990:CDS 15.0%
!!! TTTTTTCGTATCTATATTAA+AGG + chr8_3:47644877-47644896 None:intergenic 15.0%
!! CACAAATAATGAAAACAATA+AGG + chr8_3:47642460-47642479 None:intergenic 20.0%
!!! ATGAACTAGTGTTAATTTTT+TGG - chr8_3:47642490-47642509 Msa1291990:three_prime_UTR 20.0%
!!! TGAACTAGTGTTAATTTTTT+GGG - chr8_3:47642491-47642510 Msa1291990:three_prime_UTR 20.0%
!!! TTAGTACTGTTTTTGAATTT+TGG - chr8_3:47642533-47642552 Msa1291990:three_prime_UTR 20.0%
!!! TAGTACTGTTTTTGAATTTT+GGG - chr8_3:47642534-47642553 Msa1291990:three_prime_UTR 20.0%
!!! TTATTTGTGATTTTGAATTG+TGG - chr8_3:47642870-47642889 Msa1291990:intron 20.0%
!!! TATTTGTGATTTTGAATTGT+GGG - chr8_3:47642871-47642890 Msa1291990:intron 20.0%
!! AATTGGAATTTATGGATAAT+TGG + chr8_3:47643297-47643316 None:intergenic 20.0%
!! GAACAATAAATTGGAATTTA+TGG + chr8_3:47643305-47643324 None:intergenic 20.0%
!! AATTCAATTATTGATGTTGA+TGG - chr8_3:47643441-47643460 Msa1291990:intron 20.0%
!!! ATTTTGATGTTTCAATTTGT+TGG - chr8_3:47643920-47643939 Msa1291990:intron 20.0%
!! TATTATAGACTATTCTTATG+AGG + chr8_3:47644055-47644074 None:intergenic 20.0%
!!! TCTTGTATTTTAGAGAATTA+CGG + chr8_3:47644089-47644108 None:intergenic 20.0%
!! ATAATCATGAATGCTAATAT+AGG - chr8_3:47644242-47644261 Msa1291990:CDS 20.0%
!! ATGACAAAATTTCAAATGTT+GGG + chr8_3:47644299-47644318 None:intergenic 20.0%
!! AATGACAAAATTTCAAATGT+TGG + chr8_3:47644300-47644319 None:intergenic 20.0%
!!! GAAATTTTGTCATTTCAATA+TGG - chr8_3:47644306-47644325 Msa1291990:CDS 20.0%
!! AATTGAATTGAATGCAAAAA+GGG + chr8_3:47644333-47644352 None:intergenic 20.0%
!! AAATTGAATTGAATGCAAAA+AGG + chr8_3:47644334-47644353 None:intergenic 20.0%
!! AAGAAACAGTTAATAATCAT+AGG - chr8_3:47644670-47644689 Msa1291990:intron 20.0%
!! TATAGATACGAAAAAATCTA+AGG - chr8_3:47644881-47644900 Msa1291990:intron 20.0%
!! AAATAAGCAAAAAGTTTCTT+TGG - chr8_3:47645093-47645112 Msa1291990:five_prime_UTR 20.0%
!!! AGTAAGTTATATGTAAGTTT+TGG - chr8_3:47645163-47645182 Msa1291990:five_prime_UTR 20.0%
!!! GTAAGTTATATGTAAGTTTT+GGG - chr8_3:47645164-47645183 Msa1291990:five_prime_UTR 20.0%
!!! TAAGTTATATGTAAGTTTTG+GGG - chr8_3:47645165-47645184 Msa1291990:five_prime_UTR 20.0%
! AAGTTTATAACTATTTGAGG+CGG + chr8_3:47642009-47642028 None:intergenic 25.0%
! GTGAAGTTTATAACTATTTG+AGG + chr8_3:47642012-47642031 None:intergenic 25.0%
!!! TGTTTGTGTTTTTTGAATTC+TGG - chr8_3:47642383-47642402 Msa1291990:three_prime_UTR 25.0%
!!! GTTTGTGTTTTTTGAATTCT+GGG - chr8_3:47642384-47642403 Msa1291990:three_prime_UTR 25.0%
!! TTGTTTCGAATGTAATGTTT+TGG - chr8_3:47642668-47642687 Msa1291990:CDS 25.0%
!! TGTTTCGAATGTAATGTTTT+GGG - chr8_3:47642669-47642688 Msa1291990:CDS 25.0%
!! CATTATTTTCTGAGTTAACT+TGG - chr8_3:47642792-47642811 Msa1291990:CDS 25.0%
!! ATTTGTGATTTTGAATTGTG+GGG - chr8_3:47642872-47642891 Msa1291990:intron 25.0%
! TATTGTTTGGATTGTATGAA+TGG - chr8_3:47643048-47643067 Msa1291990:intron 25.0%
! CAAAACATTGATGAAATCAA+TGG + chr8_3:47643137-47643156 None:intergenic 25.0%
! AACACAATAAATGTGTCAAA+GGG + chr8_3:47643199-47643218 None:intergenic 25.0%
!! GTTTTTCTGACAATAATAAC+AGG + chr8_3:47643222-47643241 None:intergenic 25.0%
! TAGAAATTGAAAACAGGAAA+AGG + chr8_3:47643269-47643288 None:intergenic 25.0%
! AAAAACAGAGTTCATTAGAA+AGG + chr8_3:47643553-47643572 None:intergenic 25.0%
! TTGTGTGTTTGATATAGATA+AGG - chr8_3:47643572-47643591 Msa1291990:CDS 25.0%
! TCTTATTCAAATGGTTAGAA+GGG + chr8_3:47643839-47643858 None:intergenic 25.0%
! ATCTTATTCAAATGGTTAGA+AGG + chr8_3:47643840-47643859 None:intergenic 25.0%
!! ATTTGTTCTGATTGCAATTT+TGG - chr8_3:47643973-47643992 Msa1291990:intron 25.0%
! AATTTCAAATGTTGGGTATA+GGG + chr8_3:47644292-47644311 None:intergenic 25.0%
! AAATTTCAAATGTTGGGTAT+AGG + chr8_3:47644293-47644312 None:intergenic 25.0%
! AACTATGAATTCTAGATTGT+TGG - chr8_3:47644429-47644448 Msa1291990:intron 25.0%
! TAGTGTTTGTTTGTGTATAT+AGG - chr8_3:47644986-47645005 Msa1291990:intron 25.0%
! GATACTATAGTTTGGTAAAA+AGG - chr8_3:47645058-47645077 Msa1291990:CDS 25.0%
! TATAGTTTGGTAAAAAGGAA+TGG - chr8_3:47645063-47645082 Msa1291990:exon 25.0%
!! TTTTTGTAGAACTTTGAAGG+CGG + chr8_3:47641970-47641989 None:intergenic 30.0%
!! GTATGTGTGTGTTGTTTATT+GGG + chr8_3:47642093-47642112 None:intergenic 30.0%
!! TGTATGTGTGTGTTGTTTAT+TGG + chr8_3:47642094-47642113 None:intergenic 30.0%
!!! TGTTTTTGGGCTTAATTTCA+CGG + chr8_3:47642215-47642234 None:intergenic 30.0%
!! TTTTGGGAATGCAATGATTT+TGG - chr8_3:47642507-47642526 Msa1291990:three_prime_UTR 30.0%
! GTTTCGAATGTAATGTTTTG+GGG - chr8_3:47642670-47642689 Msa1291990:CDS 30.0%
!!! ATAATTTTTTTGAAGGGTGC+TGG - chr8_3:47642910-47642929 Msa1291990:intron 30.0%
TGAGTGCAATATGTATTGTT+TGG - chr8_3:47643035-47643054 Msa1291990:intron 30.0%
GAACACAATAAATGTGTCAA+AGG + chr8_3:47643200-47643219 None:intergenic 30.0%
!! AAAAACCATCACTTTTCCTT+TGG - chr8_3:47643236-47643255 Msa1291990:intron 30.0%
GTTGTGTAGAAATTGAAAAC+AGG + chr8_3:47643275-47643294 None:intergenic 30.0%
ACACATTCAATCTAAACCAT+TGG + chr8_3:47643342-47643361 None:intergenic 30.0%
! AGCTTTTCAATGCAATAACA+CGG - chr8_3:47643522-47643541 Msa1291990:CDS 30.0%
TGTGTTTGATATAGATAAGG+AGG - chr8_3:47643575-47643594 Msa1291990:CDS 30.0%
TTCATACCATGATGTGATTA+GGG - chr8_3:47643600-47643619 Msa1291990:CDS 30.0%
CTATATTATCAACAGTGCAA+GGG - chr8_3:47643666-47643685 Msa1291990:intron 30.0%
CTTATTCAAATGGTTAGAAG+GGG + chr8_3:47643838-47643857 None:intergenic 30.0%
GGGAGTTAATCTTATTCAAA+TGG + chr8_3:47643848-47643867 None:intergenic 30.0%
TGATTTAACGACCAAAAGTT+TGG + chr8_3:47643897-47643916 None:intergenic 30.0%
! AACTTTTGGTCGTTAAATCA+AGG - chr8_3:47643897-47643916 Msa1291990:intron 30.0%
!!! TGTTTTGACTTTTGAGTCTA+TGG - chr8_3:47644386-47644405 Msa1291990:intron 30.0%
GTATATAGGAAAAATCCAAC+AGG - chr8_3:47645000-47645019 Msa1291990:intron 30.0%
ATCTGTGAGATACTATAGTT+TGG - chr8_3:47645050-47645069 Msa1291990:CDS 30.0%
! GTCATTACCATCTTTAACTT+AGG + chr8_3:47645120-47645139 None:intergenic 30.0%
! GATGGTAATGACAATAGTAT+AGG - chr8_3:47645128-47645147 Msa1291990:five_prime_UTR 30.0%
GTAATGACAATAGTATAGGT+TGG - chr8_3:47645132-47645151 Msa1291990:five_prime_UTR 30.0%
GCACTCTTTACAAATATAGA+TGG - chr8_3:47645225-47645244 Msa1291990:five_prime_UTR 30.0%
! GGGTTTTTGTAGAACTTTGA+AGG + chr8_3:47641973-47641992 None:intergenic 35.0%
TGTGATGAATGTGAGAAAGT+GGG + chr8_3:47642123-47642142 None:intergenic 35.0%
TTGTGATGAATGTGAGAAAG+TGG + chr8_3:47642124-47642143 None:intergenic 35.0%
TCCAATCAAAGTCTTCAAGA+TGG - chr8_3:47642167-47642186 Msa1291990:three_prime_UTR 35.0%
GTGAAATTAAGCCCAAAAAC+AGG - chr8_3:47642214-47642233 Msa1291990:three_prime_UTR 35.0%
!! ACCATGATTCTCCTGTTTTT+GGG + chr8_3:47642228-47642247 None:intergenic 35.0%
! TACCATGATTCTCCTGTTTT+TGG + chr8_3:47642229-47642248 None:intergenic 35.0%
TGAACACACAATGAGATTCT+TGG + chr8_3:47642607-47642626 None:intergenic 35.0%
CAAATCCAAAGGAAAAGTGA+TGG + chr8_3:47643244-47643263 None:intergenic 35.0%
!! CATCACTTTTCCTTTGGATT+TGG - chr8_3:47643242-47643261 Msa1291990:intron 35.0%
GGTGGTTACGAACAATAAAT+TGG + chr8_3:47643314-47643333 None:intergenic 35.0%
GAATTGATTGCAGTTACAAG+TGG + chr8_3:47643426-47643445 None:intergenic 35.0%
CTTCATACCATGATGTGATT+AGG - chr8_3:47643599-47643618 Msa1291990:CDS 35.0%
! GAATGAGATTCAGAAAGTGT+TGG - chr8_3:47643624-47643643 Msa1291990:CDS 35.0%
!! CCTTGCACTGTTGATAATAT+AGG + chr8_3:47643668-47643687 None:intergenic 35.0%
CCTATATTATCAACAGTGCA+AGG - chr8_3:47643665-47643684 Msa1291990:intron 35.0%
TTTAACGACCAAAAGTTTGG+TGG + chr8_3:47643894-47643913 None:intergenic 35.0%
ACCTCTATGTTAATGCATTG+AGG - chr8_3:47644007-47644026 Msa1291990:intron 35.0%
ACCTCAATGCATTAACATAG+AGG + chr8_3:47644011-47644030 None:intergenic 35.0%
GGTCACATCAACTACATTAT+TGG - chr8_3:47644028-47644047 Msa1291990:intron 35.0%
TTTCAAGCTAGCACTTAAAG+TGG - chr8_3:47644206-47644225 Msa1291990:CDS 35.0%
TTCAAGCTAGCACTTAAAGT+GGG - chr8_3:47644207-47644226 Msa1291990:CDS 35.0%
AGTCTGATATCATTGAAGCA+CGG + chr8_3:47644491-47644510 None:intergenic 35.0%
TTCAATGATATCAGACTCAG+AGG - chr8_3:47644494-47644513 Msa1291990:intron 35.0%
TAATGTTCTTCAGTCTTCCA+TGG + chr8_3:47644542-47644561 None:intergenic 35.0%
TGGAAGACTGAAGAACATTA+AGG - chr8_3:47644542-47644561 Msa1291990:intron 35.0%
AGATCAGCCAAAAGAAGAAA+GGG - chr8_3:47644609-47644628 Msa1291990:intron 35.0%
! AGGAATTCCCTTTCTTCTTT+TGG + chr8_3:47644619-47644638 None:intergenic 35.0%
CAAGAAATCTCTAACTGTAG+GGG + chr8_3:47644728-47644747 None:intergenic 35.0%
GCAAGAAATCTCTAACTGTA+GGG + chr8_3:47644729-47644748 None:intergenic 35.0%
TGCAAGAAATCTCTAACTGT+AGG + chr8_3:47644730-47644749 None:intergenic 35.0%
AGTGGTTGTTCGATCAAAAT+TGG + chr8_3:47644800-47644819 None:intergenic 35.0%
!! CTAGAGCTTTTTGGTGAAAA+GGG - chr8_3:47644938-47644957 Msa1291990:intron 35.0%
TTGGTAAAAAGGAATGGTGT+AGG - chr8_3:47645069-47645088 Msa1291990:exon 35.0%
CTTTGGACCTAAGTTAAAGA+TGG - chr8_3:47645110-47645129 Msa1291990:five_prime_UTR 35.0%
!!! AGTTTTGGGGATGTAAATAG+TGG - chr8_3:47645178-47645197 Msa1291990:five_prime_UTR 35.0%
GCCATCTTGAAGACTTTGAT+TGG + chr8_3:47642171-47642190 None:intergenic 40.0%
TTAGCTCACACTCTCATTGT+TGG - chr8_3:47642291-47642310 Msa1291990:three_prime_UTR 40.0%
!!! ATTTTTTTGAAGGGTGCTGG+AGG - chr8_3:47642913-47642932 Msa1291990:intron 40.0%
TTGAGAAGAGGTTTCAACCA+TGG + chr8_3:47642967-47642986 None:intergenic 40.0%
! AAGAAACGCTCTTTGAGAAG+AGG + chr8_3:47642979-47642998 None:intergenic 40.0%
GAATAGCACGATGATCTTTG+TGG + chr8_3:47643101-47643120 None:intergenic 40.0%
! CAAAGATCATCGTGCTATTC+AGG - chr8_3:47643101-47643120 Msa1291990:intron 40.0%
AGGAAAAGGACCAAATCCAA+AGG + chr8_3:47643255-47643274 None:intergenic 40.0%
ACACTAACAACGACATGTGT+CGG + chr8_3:47643374-47643393 None:intergenic 40.0%
CAGACACCAACATCAAATGT+CGG + chr8_3:47643403-47643422 None:intergenic 40.0%
! TTGATGGTCATTGTTGTGTC+TGG - chr8_3:47643457-47643476 Msa1291990:intron 40.0%
CATTCACCCTAATCACATCA+TGG + chr8_3:47643609-47643628 None:intergenic 40.0%
!! CAGAAAGTGTTGGACATAAC+AGG - chr8_3:47643634-47643653 Msa1291990:CDS 40.0%
AGAATCGAAAGGAATCGAGA+AGG + chr8_3:47643768-47643787 None:intergenic 40.0%
TCCTTTCGATTCTGTTCTCT+TGG - chr8_3:47643775-47643794 Msa1291990:intron 40.0%
AAGGGGAAGACATTATGTGA+AGG + chr8_3:47643821-47643840 None:intergenic 40.0%
! ATAATCCGCCACCAAACTTT+TGG - chr8_3:47643883-47643902 Msa1291990:intron 40.0%
!! GACTTTTGAGTCTATGGCAT+TGG - chr8_3:47644392-47644411 Msa1291990:intron 40.0%
GTCTGATATCATTGAAGCAC+GGG + chr8_3:47644490-47644509 None:intergenic 40.0%
GATCTGTAATTGACTGTTGG+TGG + chr8_3:47644594-47644613 None:intergenic 40.0%
GCTGATCTGTAATTGACTGT+TGG + chr8_3:47644597-47644616 None:intergenic 40.0%
CAGATCAGCCAAAAGAAGAA+AGG - chr8_3:47644608-47644627 Msa1291990:intron 40.0%
AGTATACTAGTCCTCCCATT+GGG + chr8_3:47644650-47644669 None:intergenic 40.0%
TAGTATACTAGTCCTCCCAT+TGG + chr8_3:47644651-47644670 None:intergenic 40.0%
ATTTCTTGCATTCACGCAGT+TGG - chr8_3:47644740-47644759 Msa1291990:intron 40.0%
TTGGTCTATCCAGTCTTGAT+CGG + chr8_3:47644781-47644800 None:intergenic 40.0%
TCACTTAGTCGAGACATTAG+TGG + chr8_3:47644818-47644837 None:intergenic 40.0%
ATGTCTCGACTAAGTGAATG+CGG - chr8_3:47644821-47644840 Msa1291990:intron 40.0%
ATCAGATTCCCTTCAGTCAA+GGG + chr8_3:47644850-47644869 None:intergenic 40.0%
AATCAGATTCCCTTCAGTCA+AGG + chr8_3:47644851-47644870 None:intergenic 40.0%
!! GCTAGAGCTTTTTGGTGAAA+AGG - chr8_3:47644937-47644956 Msa1291990:intron 40.0%
TAGGAAAAATCCAACAGGAG+TGG - chr8_3:47645005-47645024 Msa1291990:intron 40.0%
!! CTAACTTTTTCCACTCCTGT+TGG + chr8_3:47645018-47645037 None:intergenic 40.0%
CAACAGGAGTGGAAAAAGTT+AGG - chr8_3:47645016-47645035 Msa1291990:intron 40.0%
! CTATTTGAGGCGGTGAAAGA+GGG + chr8_3:47641999-47642018 None:intergenic 45.0%
! ACTATTTGAGGCGGTGAAAG+AGG + chr8_3:47642000-47642019 None:intergenic 45.0%
GCCCAAAAACAGGAGAATCA+TGG - chr8_3:47642224-47642243 Msa1291990:three_prime_UTR 45.0%
! GTCCTGATGATGAGGAAAAC+AGG - chr8_3:47642935-47642954 Msa1291990:intron 45.0%
! CACCTGTTTTCCTCATCATC+AGG + chr8_3:47642940-47642959 None:intergenic 45.0%
! CTGATGATGAGGAAAACAGG+TGG - chr8_3:47642938-47642957 Msa1291990:intron 45.0%
AGAAGAGGTTTCAACCATGG+TGG + chr8_3:47642964-47642983 None:intergenic 45.0%
CAATCTAAACCATTGGTGCG+AGG + chr8_3:47643335-47643354 None:intergenic 45.0%
CAGACACCGACATTTGATGT+TGG - chr8_3:47643394-47643413 Msa1291990:intron 45.0%
GGGTTGTGTTCTTGAATGAG+AGG - chr8_3:47643686-47643705 Msa1291990:intron 45.0%
!!! AGTGTTGGTGACACCTTTTC+CGG + chr8_3:47643725-47643744 None:intergenic 45.0%
GCCAAGAGAACAGAATCGAA+AGG + chr8_3:47643779-47643798 None:intergenic 45.0%
ATTCTGTTCTCTTGGCTGCA+AGG - chr8_3:47643783-47643802 Msa1291990:intron 45.0%
! AACGACCAAAAGTTTGGTGG+CGG + chr8_3:47643891-47643910 None:intergenic 45.0%
! CTGTAATTGACTGTTGGTGG+TGG + chr8_3:47644591-47644610 None:intergenic 45.0%
GTATACTAGTCCTCCCATTG+GGG + chr8_3:47644649-47644668 None:intergenic 45.0%
AGGGGAGTTCGACTCATTAA+GGG + chr8_3:47644710-47644729 None:intergenic 45.0%
TAGGGGAGTTCGACTCATTA+AGG + chr8_3:47644711-47644730 None:intergenic 45.0%
CATTCACGCAGTTGGAACAT+TGG - chr8_3:47644748-47644767 Msa1291990:intron 45.0%
TGTCTCGACTAAGTGAATGC+GGG - chr8_3:47644822-47644841 Msa1291990:intron 45.0%
! GTTCCAGTGCTAGAGCTTTT+TGG - chr8_3:47644929-47644948 Msa1291990:intron 45.0%
! TCACCAAAAAGCTCTAGCAC+TGG + chr8_3:47644935-47644954 None:intergenic 45.0%
TCTAAACCATTGGTGCGAGG+TGG + chr8_3:47643332-47643351 None:intergenic 50.0%
!!! GTGTTGGTGACACCTTTTCC+GGG + chr8_3:47643724-47643743 None:intergenic 50.0%
CTCGCAGACTTCACAAGTGT+TGG + chr8_3:47643740-47643759 None:intergenic 50.0%
!! GGGCTGACTTGTTCTTCTTC+TGG + chr8_3:47644470-47644489 None:intergenic 50.0%
!! ACTGTTGGTGGTGGTGTTGA+AGG + chr8_3:47644582-47644601 None:intergenic 50.0%
AATTCCTCATCGTGCCCCAA+TGG - chr8_3:47644632-47644651 Msa1291990:intron 50.0%
ATTCCTCATCGTGCCCCAAT+GGG - chr8_3:47644633-47644652 Msa1291990:intron 50.0%
GGTGATCATCCGATCAAGAC+TGG - chr8_3:47644769-47644788 Msa1291990:intron 50.0%
GAGGCGGTGAAAGAGGGAAT+GGG + chr8_3:47641993-47642012 None:intergenic 55.0%
TGAGGCGGTGAAAGAGGGAA+TGG + chr8_3:47641994-47642013 None:intergenic 55.0%
! TGCTGGAGGTCCTGATGATG+AGG - chr8_3:47642927-47642946 Msa1291990:intron 55.0%
AGGAAAACAGGTGGCCACCA+TGG - chr8_3:47642947-47642966 Msa1291990:intron 55.0%
TCGTAACCACCTCGCACCAA+TGG - chr8_3:47643323-47643342 Msa1291990:intron 55.0%
TGAATGAGAGGCCTCAGCCA+AGG - chr8_3:47643698-47643717 Msa1291990:intron 55.0%
ATGCGGGATCCCTTGACTGA+AGG - chr8_3:47644838-47644857 Msa1291990:intron 55.0%
TGCGGGATCCCTTGACTGAA+GGG - chr8_3:47644839-47644858 Msa1291990:intron 55.0%
TTGGTGAAAAGGGCCAGTGC+TGG - chr8_3:47644948-47644967 Msa1291990:intron 55.0%
AACGACAGCACTTCCAGCAC+TGG + chr8_3:47644964-47644983 None:intergenic 55.0%
! GTGACACCTTTTCCGGGCCT+TGG + chr8_3:47643718-47643737 None:intergenic 60.0%
CACAGCAGCAGTTGCAGCCA+TGG - chr8_3:47644522-47644541 Msa1291990:intron 60.0%
! CCTTTTCCGGGCCTTGGCTG+AGG + chr8_3:47643712-47643731 None:intergenic 65.0%
CCTCAGCCAAGGCCCGGAAA+AGG - chr8_3:47643709-47643728 Msa1291990:intron 65.0%
CCTCCCATTGGGGCACGATG+AGG + chr8_3:47644639-47644658 None:intergenic 65.0%
CCTCATCGTGCCCCAATGGG+AGG - chr8_3:47644636-47644655 Msa1291990:intron 65.0%
GAGAGGCCTCAGCCAAGGCC+CGG - chr8_3:47643703-47643722 Msa1291990:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 47641945 47645319 47641945 ID=Msa1291990;Name=Msa1291990
chr8_3 mRNA 47641945 47645319 47641945 ID=Msa1291990-mRNA-1;Parent=Msa1291990;Name=Msa1291990-mRNA-1;_AED=0.02;_eAED=0.02;_QI=241|1|1|1|1|1|4|648|233
chr8_3 exon 47641945 47642820 47641945 ID=Msa1291990-mRNA-1:exon:13273;Parent=Msa1291990-mRNA-1
chr8_3 exon 47643460 47643675 47643460 ID=Msa1291990-mRNA-1:exon:13272;Parent=Msa1291990-mRNA-1
chr8_3 exon 47644142 47644339 47644142 ID=Msa1291990-mRNA-1:exon:13271;Parent=Msa1291990-mRNA-1
chr8_3 exon 47645019 47645319 47645019 ID=Msa1291990-mRNA-1:exon:13270;Parent=Msa1291990-mRNA-1
chr8_3 five_prime_UTR 47645079 47645319 47645079 ID=Msa1291990-mRNA-1:five_prime_utr;Parent=Msa1291990-mRNA-1
chr8_3 CDS 47645019 47645078 47645019 ID=Msa1291990-mRNA-1:cds;Parent=Msa1291990-mRNA-1
chr8_3 CDS 47644142 47644339 47644142 ID=Msa1291990-mRNA-1:cds;Parent=Msa1291990-mRNA-1
chr8_3 CDS 47643460 47643675 47643460 ID=Msa1291990-mRNA-1:cds;Parent=Msa1291990-mRNA-1
chr8_3 CDS 47642593 47642820 47642593 ID=Msa1291990-mRNA-1:cds;Parent=Msa1291990-mRNA-1
chr8_3 three_prime_UTR 47641945 47642592 47641945 ID=Msa1291990-mRNA-1:three_prime_utr;Parent=Msa1291990-mRNA-1
Gene Sequence

>Msa1291990

ATGGCGTTTGAAAATCAAGACACTGTCCGTGAAATTAAGCCCAAAAACAGGAGAATCATGGGTGCTGGAGGTCCTGATGATGAGGAAAACAGGTGGCCACCATGGTTGAAACCTCTTCTCAAAGAGCGTTTCTTTGTTCAATGCAAGTTGCATGCTGATTCACACAAGAGTGAGTGCAATATGTATTGTTTGGATTGTATGAATGGTGCTCTTTGCTCACTCTGTCTCAATTACCACAAAGATCATCGTGCTATTCAGATAAGGAGGTCTTCATACCATGATGTGATTAGGGTGAATGAGATTCAGAAAGTGTTGGACATAACAGGTGTTCAAACCTATATTATCAACAGTGCAAGGGTTGTGTTCTTGAATGAGAGGCCTCAGCCAAGGCCCGGAAAAGGTGTCACCAACACTTGTGAAGTCTGCGAGCGTAGCCTTCTCGATTCCTTTCGATTCTGTTCTCTTGGCTGCAAGATTGTTGGAACTTCAATGAATTTCCAGAAGAAGAACAAGTCAGCCCGTGCTTCAATGATATCAGACTCAGAGGATTCACACAGCAGCAGTTGCAGCCATGGAAGACTGAAGAACATTAAGGTGCAAAGCTTCACTCCTTCAACACCACCACCAACAGTCAATTACAGATCAGCCAAAAGAAGAAAGGGAATTCCTCATCGTGCCCCAATGGGAGGACTAGTATACTAA
Protein sequence

>Msa1291990

MAFENQDTVREIKPKNRRIMGAGGPDDEENRWPPWLKPLLKERFFVQCKLHADSHKSECNMYCLDCMNGALCSLCLNYHKDHRAIQIRRSSYHDVIRVNEIQKVLDITGVQTYIINSARVVFLNERPQPRPGKGVTNTCEVCERSLLDSFRFCSLGCKIVGTSMNFQKKNKSARASMISDSEDSHSSSCSHGRLKNIKVQSFTPSTPPPTVNYRSAKRRKGIPHRAPMGGLVY*