Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198210 | KEH20116.1 | 94.937 | 79 | 4 | 0 | 2 | 80 | 277 | 355 | 1.75e-44 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198210 | sp|Q6DYE5|U496K_ARATH | 56.579 | 76 | 30 | 1 | 5 | 80 | 294 | 366 | 8.37e-21 | 87.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198210 | A0A072TSC9 | 94.937 | 79 | 4 | 0 | 2 | 80 | 277 | 355 | 8.37e-45 | 157 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1198210 | Msa1198220 | 0.955877 | 1.098056e-113 | -8.615850e-47 |
| Msa1198210 | Msa1198230 | 0.969626 | 2.123177e-130 | -8.615850e-47 |
| Msa1198210 | Msa1292400 | 0.806875 | 6.649944e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198210 | MtrunA17_Chr8g0367331 | 94.937 | 79 | 4 | 0 | 2 | 80 | 273 | 351 | 1.70e-48 | 157 |
| Msa1198210 | MtrunA17_Chr1g0157041 | 88.608 | 79 | 8 | 1 | 2 | 80 | 267 | 344 | 1.19e-37 | 128 |
| Msa1198210 | MtrunA17_Chr3g0143271 | 65.823 | 79 | 25 | 1 | 4 | 80 | 275 | 353 | 1.94e-28 | 104 |
| Msa1198210 | MtrunA17_Chr1g0202781 | 43.421 | 76 | 42 | 1 | 5 | 80 | 267 | 341 | 4.28e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1198210 | AT1G20180.2 | 56.579 | 76 | 30 | 1 | 5 | 80 | 263 | 335 | 7.11e-22 | 87.0 |
| Msa1198210 | AT1G20180.1 | 56.579 | 76 | 30 | 1 | 5 | 80 | 294 | 366 | 8.51e-22 | 87.0 |
| Msa1198210 | AT3G49070.1 | 46.053 | 76 | 40 | 1 | 5 | 80 | 304 | 378 | 2.05e-13 | 63.9 |
| Msa1198210 | AT3G49070.2 | 46.053 | 76 | 40 | 1 | 5 | 80 | 264 | 338 | 3.80e-13 | 63.2 |
Find 10 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAAATGTGAAATATTGAAT+AGG | 0.374037 | 8_1:+49566809 | Msa1198210:CDS |
| CAACTTGATGCTGCTGCAAA+AGG | 0.382116 | 8_1:+49566670 | Msa1198210:CDS |
| AACTTGATGCTGCTGCAAAA+GGG | 0.438252 | 8_1:+49566671 | Msa1198210:CDS |
| TAAATGTGAAATATTGAATA+GGG | 0.473542 | 8_1:+49566810 | Msa1198210:CDS |
| ACTTGATGCTGCTGCAAAAG+GGG | 0.547139 | 8_1:+49566672 | Msa1198210:CDS |
| CTATGAGTAGGATGGTGAAG+AGG | 0.584575 | 8_1:+49566722 | Msa1198210:CDS |
| ATGATTTGGATACTATGAGT+AGG | 0.598596 | 8_1:+49566710 | Msa1198210:CDS |
| GTTTGTGTGAAGAATAATGT+TGG | 0.598996 | 8_1:+49566784 | Msa1198210:CDS |
| GAAGAGGTTGAATGATGAAG+TGG | 0.627772 | 8_1:+49566738 | Msa1198210:CDS |
| TTTGGATACTATGAGTAGGA+TGG | 0.662243 | 8_1:+49566714 | Msa1198210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATATTGATAAATGATT+TGG | + | chr8_1:49566696-49566715 | Msa1198210:CDS | 10.0% |
| !!! | ATTAAATTAAAATCTTTTAG+TGG | + | chr8_1:49567286-49567305 | Msa1198210:intron | 10.0% |
| !!! | TTTTATATTGATAAATGATT+TGG | + | chr8_1:49566696-49566715 | Msa1198210:CDS | 10.0% |
| !!! | ATTAAATTAAAATCTTTTAG+TGG | + | chr8_1:49567286-49567305 | Msa1198210:intron | 10.0% |
| !! | TAAATGTGAAATATTGAATA+GGG | + | chr8_1:49566810-49566829 | Msa1198210:CDS | 15.0% |
| !! | TTACAGAGATAAATAAATAT+AGG | + | chr8_1:49566953-49566972 | Msa1198210:intron | 15.0% |
| !! | TACAGAGATAAATAAATATA+GGG | + | chr8_1:49566954-49566973 | Msa1198210:intron | 15.0% |
| !!! | TAATTTTTGTTTCTTTTCTT+TGG | + | chr8_1:49567041-49567060 | Msa1198210:intron | 15.0% |
| !! | GAACAAATGATTAAATAAAA+TGG | + | chr8_1:49567356-49567375 | Msa1198210:intron | 15.0% |
| !! | TAAATGTGAAATATTGAATA+GGG | + | chr8_1:49566810-49566829 | Msa1198210:CDS | 15.0% |
| !! | TTACAGAGATAAATAAATAT+AGG | + | chr8_1:49566953-49566972 | Msa1198210:intron | 15.0% |
| !! | TACAGAGATAAATAAATATA+GGG | + | chr8_1:49566954-49566973 | Msa1198210:intron | 15.0% |
| !!! | TAATTTTTGTTTCTTTTCTT+TGG | + | chr8_1:49567041-49567060 | Msa1198210:intron | 15.0% |
| !! | GAACAAATGATTAAATAAAA+TGG | + | chr8_1:49567356-49567375 | Msa1198210:intron | 15.0% |
| !! | GTAAATGTGAAATATTGAAT+AGG | + | chr8_1:49566809-49566828 | Msa1198210:CDS | 20.0% |
| !! | AAATAGAAGAGAAATAAGAA+AGG | + | chr8_1:49567528-49567547 | Msa1198210:intron | 20.0% |
| !! | AATAGAAGAGAAATAAGAAA+GGG | + | chr8_1:49567529-49567548 | Msa1198210:intron | 20.0% |
| !! | GTAAATGTGAAATATTGAAT+AGG | + | chr8_1:49566809-49566828 | Msa1198210:CDS | 20.0% |
| !! | AAATAGAAGAGAAATAAGAA+AGG | + | chr8_1:49567528-49567547 | Msa1198210:intron | 20.0% |
| !! | AATAGAAGAGAAATAAGAAA+GGG | + | chr8_1:49567529-49567548 | Msa1198210:intron | 20.0% |
| ! | AAAGTCACATACAAAATTAG+TGG | + | chr8_1:49567104-49567123 | Msa1198210:intron | 25.0% |
| ! | TTAGCAAATTAATTATGTGC+TGG | - | chr8_1:49567267-49567286 | None:intergenic | 25.0% |
| ! | ATCAATTTGCAAAACAAGAA+GGG | - | chr8_1:49567505-49567524 | None:intergenic | 25.0% |
| ! | AATCAATTTGCAAAACAAGA+AGG | - | chr8_1:49567506-49567525 | None:intergenic | 25.0% |
| !!! | TTCTTGTTTTGCAAATTGAT+TGG | + | chr8_1:49567505-49567524 | Msa1198210:intron | 25.0% |
| !! | AGATGGTTTGAATATTGTTT+TGG | - | chr8_1:49567606-49567625 | None:intergenic | 25.0% |
| ! | AAAGTCACATACAAAATTAG+TGG | + | chr8_1:49567104-49567123 | Msa1198210:intron | 25.0% |
| ! | TTAGCAAATTAATTATGTGC+TGG | - | chr8_1:49567267-49567286 | None:intergenic | 25.0% |
| ! | ATCAATTTGCAAAACAAGAA+GGG | - | chr8_1:49567505-49567524 | None:intergenic | 25.0% |
| ! | AATCAATTTGCAAAACAAGA+AGG | - | chr8_1:49567506-49567525 | None:intergenic | 25.0% |
| !!! | TTCTTGTTTTGCAAATTGAT+TGG | + | chr8_1:49567505-49567524 | Msa1198210:intron | 25.0% |
| !! | AGATGGTTTGAATATTGTTT+TGG | - | chr8_1:49567606-49567625 | None:intergenic | 25.0% |
| ! | ATGATTTGGATACTATGAGT+AGG | + | chr8_1:49566710-49566729 | Msa1198210:CDS | 30.0% |
| TGAAGTGGAACATAGAAAAA+TGG | + | chr8_1:49566753-49566772 | Msa1198210:CDS | 30.0% | |
| GTTTGTGTGAAGAATAATGT+TGG | + | chr8_1:49566784-49566803 | Msa1198210:CDS | 30.0% | |
| TTCTTACCATCAACAAATCT+AGG | + | chr8_1:49566911-49566930 | Msa1198210:intron | 30.0% | |
| AAATATAGGGAAATGCTAAC+CGG | + | chr8_1:49566967-49566986 | Msa1198210:intron | 30.0% | |
| ! | AAAATTAGTGGATCAAGTAG+AGG | + | chr8_1:49567116-49567135 | Msa1198210:intron | 30.0% |
| CGTAGTTCATATCTTTAGTT+CGG | - | chr8_1:49567195-49567214 | None:intergenic | 30.0% | |
| CTAAAGATATGAACTACGTT+TGG | + | chr8_1:49567197-49567216 | Msa1198210:intron | 30.0% | |
| ! | AGCTTGGACACTATTTTATA+CGG | - | chr8_1:49567223-49567242 | None:intergenic | 30.0% |
| TCAATTTGCAAAACAAGAAG+GGG | - | chr8_1:49567504-49567523 | None:intergenic | 30.0% | |
| CAAATTGATGTACGTTTAGA+TGG | - | chr8_1:49567623-49567642 | None:intergenic | 30.0% | |
| CATCTAAACGTACATCAATT+TGG | + | chr8_1:49567621-49567640 | Msa1198210:intron | 30.0% | |
| ! | ATGATTTGGATACTATGAGT+AGG | + | chr8_1:49566710-49566729 | Msa1198210:CDS | 30.0% |
| TGAAGTGGAACATAGAAAAA+TGG | + | chr8_1:49566753-49566772 | Msa1198210:CDS | 30.0% | |
| GTTTGTGTGAAGAATAATGT+TGG | + | chr8_1:49566784-49566803 | Msa1198210:CDS | 30.0% | |
| TTCTTACCATCAACAAATCT+AGG | + | chr8_1:49566911-49566930 | Msa1198210:intron | 30.0% | |
| AAATATAGGGAAATGCTAAC+CGG | + | chr8_1:49566967-49566986 | Msa1198210:intron | 30.0% | |
| ! | AAAATTAGTGGATCAAGTAG+AGG | + | chr8_1:49567116-49567135 | Msa1198210:intron | 30.0% |
| CGTAGTTCATATCTTTAGTT+CGG | - | chr8_1:49567195-49567214 | None:intergenic | 30.0% | |
| CTAAAGATATGAACTACGTT+TGG | + | chr8_1:49567197-49567216 | Msa1198210:intron | 30.0% | |
| ! | AGCTTGGACACTATTTTATA+CGG | - | chr8_1:49567223-49567242 | None:intergenic | 30.0% |
| TCAATTTGCAAAACAAGAAG+GGG | - | chr8_1:49567504-49567523 | None:intergenic | 30.0% | |
| CAAATTGATGTACGTTTAGA+TGG | - | chr8_1:49567623-49567642 | None:intergenic | 30.0% | |
| CATCTAAACGTACATCAATT+TGG | + | chr8_1:49567621-49567640 | Msa1198210:intron | 30.0% | |
| TTTGGATACTATGAGTAGGA+TGG | + | chr8_1:49566714-49566733 | Msa1198210:CDS | 35.0% | |
| !!! | AGATCAAGAGTCTAGCTTTT+TGG | + | chr8_1:49566849-49566868 | Msa1198210:CDS | 35.0% |
| !! | AAGTCTCCTAGATTTGTTGA+TGG | - | chr8_1:49566920-49566939 | None:intergenic | 35.0% |
| CTACGTATATACCTCTTCTA+GGG | - | chr8_1:49567077-49567096 | None:intergenic | 35.0% | |
| ACTACGTATATACCTCTTCT+AGG | - | chr8_1:49567078-49567097 | None:intergenic | 35.0% | |
| AACAATCATCTCTTTCTCCA+CGG | + | chr8_1:49567454-49567473 | Msa1198210:intron | 35.0% | |
| CAATTTGCAAAACAAGAAGG+GGG | - | chr8_1:49567503-49567522 | None:intergenic | 35.0% | |
| TTTGGATACTATGAGTAGGA+TGG | + | chr8_1:49566714-49566733 | Msa1198210:CDS | 35.0% | |
| !!! | AGATCAAGAGTCTAGCTTTT+TGG | + | chr8_1:49566849-49566868 | Msa1198210:CDS | 35.0% |
| !! | AAGTCTCCTAGATTTGTTGA+TGG | - | chr8_1:49566920-49566939 | None:intergenic | 35.0% |
| CTACGTATATACCTCTTCTA+GGG | - | chr8_1:49567077-49567096 | None:intergenic | 35.0% | |
| ACTACGTATATACCTCTTCT+AGG | - | chr8_1:49567078-49567097 | None:intergenic | 35.0% | |
| AACAATCATCTCTTTCTCCA+CGG | + | chr8_1:49567454-49567473 | Msa1198210:intron | 35.0% | |
| CAATTTGCAAAACAAGAAGG+GGG | - | chr8_1:49567503-49567522 | None:intergenic | 35.0% | |
| AACTTGATGCTGCTGCAAAA+GGG | + | chr8_1:49566671-49566690 | Msa1198210:CDS | 40.0% | |
| GAAGAGGTTGAATGATGAAG+TGG | + | chr8_1:49566738-49566757 | Msa1198210:CDS | 40.0% | |
| !!! | GTCTAGCTTTTTGGATCAGT+TGG | + | chr8_1:49566858-49566877 | Msa1198210:CDS | 40.0% |
| TACTTCCTTAACCTGTACTC+CGG | - | chr8_1:49566996-49567015 | None:intergenic | 40.0% | |
| !!! | CTTGTTGAGCACTGCTTTTT+GGG | + | chr8_1:49567241-49567260 | Msa1198210:intron | 40.0% |
| ! | CTTGTGTCATTAGAGTGCAT+TGG | + | chr8_1:49567388-49567407 | Msa1198210:intron | 40.0% |
| CATTGGAAACATGTACCTCT+AGG | + | chr8_1:49567405-49567424 | Msa1198210:intron | 40.0% | |
| TCGAACTAGTACATGCTTAG+CGG | - | chr8_1:49567568-49567587 | None:intergenic | 40.0% | |
| AACTTGATGCTGCTGCAAAA+GGG | + | chr8_1:49566671-49566690 | Msa1198210:CDS | 40.0% | |
| GAAGAGGTTGAATGATGAAG+TGG | + | chr8_1:49566738-49566757 | Msa1198210:CDS | 40.0% | |
| !!! | GTCTAGCTTTTTGGATCAGT+TGG | + | chr8_1:49566858-49566877 | Msa1198210:CDS | 40.0% |
| TACTTCCTTAACCTGTACTC+CGG | - | chr8_1:49566996-49567015 | None:intergenic | 40.0% | |
| !!! | CTTGTTGAGCACTGCTTTTT+GGG | + | chr8_1:49567241-49567260 | Msa1198210:intron | 40.0% |
| ! | CTTGTGTCATTAGAGTGCAT+TGG | + | chr8_1:49567388-49567407 | Msa1198210:intron | 40.0% |
| CATTGGAAACATGTACCTCT+AGG | + | chr8_1:49567405-49567424 | Msa1198210:intron | 40.0% | |
| TCGAACTAGTACATGCTTAG+CGG | - | chr8_1:49567568-49567587 | None:intergenic | 40.0% | |
| CAACTTGATGCTGCTGCAAA+AGG | + | chr8_1:49566670-49566689 | Msa1198210:CDS | 45.0% | |
| ACTTGATGCTGCTGCAAAAG+GGG | + | chr8_1:49566672-49566691 | Msa1198210:CDS | 45.0% | |
| CTATGAGTAGGATGGTGAAG+AGG | + | chr8_1:49566722-49566741 | Msa1198210:CDS | 45.0% | |
| ACTTCCTTAACCTGTACTCC+GGG | - | chr8_1:49566995-49567014 | None:intergenic | 45.0% | |
| GTTCTTCTAGACCCTAGAAG+AGG | + | chr8_1:49567063-49567082 | Msa1198210:intron | 45.0% | |
| AAAGCAGTGCTCAACAAGCT+TGG | - | chr8_1:49567239-49567258 | None:intergenic | 45.0% | |
| !! | GCTTGTTGAGCACTGCTTTT+TGG | + | chr8_1:49567240-49567259 | Msa1198210:intron | 45.0% |
| AAAACAAGAAGGGGGTCACT+GGG | - | chr8_1:49567495-49567514 | None:intergenic | 45.0% | |
| CAACTTGATGCTGCTGCAAA+AGG | + | chr8_1:49566670-49566689 | Msa1198210:CDS | 45.0% | |
| ACTTGATGCTGCTGCAAAAG+GGG | + | chr8_1:49566672-49566691 | Msa1198210:CDS | 45.0% | |
| CTATGAGTAGGATGGTGAAG+AGG | + | chr8_1:49566722-49566741 | Msa1198210:CDS | 45.0% | |
| ACTTCCTTAACCTGTACTCC+GGG | - | chr8_1:49566995-49567014 | None:intergenic | 45.0% | |
| GTTCTTCTAGACCCTAGAAG+AGG | + | chr8_1:49567063-49567082 | Msa1198210:intron | 45.0% | |
| AAAGCAGTGCTCAACAAGCT+TGG | - | chr8_1:49567239-49567258 | None:intergenic | 45.0% | |
| !! | GCTTGTTGAGCACTGCTTTT+TGG | + | chr8_1:49567240-49567259 | Msa1198210:intron | 45.0% |
| AAAACAAGAAGGGGGTCACT+GGG | - | chr8_1:49567495-49567514 | None:intergenic | 45.0% | |
| TGCACGACAACCATGGTTGA+CGG | - | chr8_1:49567431-49567450 | None:intergenic | 50.0% | |
| TGTTGCATGCACGACAACCA+TGG | - | chr8_1:49567438-49567457 | None:intergenic | 50.0% | |
| CAAAACAAGAAGGGGGTCAC+TGG | - | chr8_1:49567496-49567515 | None:intergenic | 50.0% | |
| TGCACGACAACCATGGTTGA+CGG | - | chr8_1:49567431-49567450 | None:intergenic | 50.0% | |
| TGTTGCATGCACGACAACCA+TGG | - | chr8_1:49567438-49567457 | None:intergenic | 50.0% | |
| CAAAACAAGAAGGGGGTCAC+TGG | - | chr8_1:49567496-49567515 | None:intergenic | 50.0% | |
| TTAACCTGTACTCCGGGCAC+CGG | - | chr8_1:49566989-49567008 | None:intergenic | 55.0% | |
| TACCTCTAGGCCGTCAACCA+TGG | + | chr8_1:49567418-49567437 | Msa1198210:intron | 55.0% | |
| AACCATGGTTGACGGCCTAG+AGG | - | chr8_1:49567423-49567442 | None:intergenic | 55.0% | |
| !! | GGACAAGGAAGGTGTTACCG+TGG | - | chr8_1:49567474-49567493 | None:intergenic | 55.0% |
| TAGTACATGCTTAGCGGCGC+GGG | - | chr8_1:49567562-49567581 | None:intergenic | 55.0% | |
| CTAGTACATGCTTAGCGGCG+CGG | - | chr8_1:49567563-49567582 | None:intergenic | 55.0% | |
| TTAACCTGTACTCCGGGCAC+CGG | - | chr8_1:49566989-49567008 | None:intergenic | 55.0% | |
| TACCTCTAGGCCGTCAACCA+TGG | + | chr8_1:49567418-49567437 | Msa1198210:intron | 55.0% | |
| AACCATGGTTGACGGCCTAG+AGG | - | chr8_1:49567423-49567442 | None:intergenic | 55.0% | |
| !! | GGACAAGGAAGGTGTTACCG+TGG | - | chr8_1:49567474-49567493 | None:intergenic | 55.0% |
| TAGTACATGCTTAGCGGCGC+GGG | - | chr8_1:49567562-49567581 | None:intergenic | 55.0% | |
| CTAGTACATGCTTAGCGGCG+CGG | - | chr8_1:49567563-49567582 | None:intergenic | 55.0% | |
| GGGAAATGCTAACCGGTGCC+CGG | + | chr8_1:49566974-49566993 | Msa1198210:intron | 60.0% | |
| GGTGCCCGGAGTACAGGTTA+AGG | + | chr8_1:49566988-49567007 | Msa1198210:intron | 60.0% | |
| AGAAGGGGGTCACTGGGACA+AGG | - | chr8_1:49567489-49567508 | None:intergenic | 60.0% | |
| GGGAAATGCTAACCGGTGCC+CGG | + | chr8_1:49566974-49566993 | Msa1198210:intron | 60.0% | |
| GGTGCCCGGAGTACAGGTTA+AGG | + | chr8_1:49566988-49567007 | Msa1198210:intron | 60.0% | |
| AGAAGGGGGTCACTGGGACA+AGG | - | chr8_1:49567489-49567508 | None:intergenic | 60.0% | |
| CTAACCGGTGCCCGGAGTAC+AGG | + | chr8_1:49566982-49567001 | Msa1198210:intron | 65.0% | |
| GGGGGTCACTGGGACAAGGA+AGG | - | chr8_1:49567485-49567504 | None:intergenic | 65.0% | |
| CTAACCGGTGCCCGGAGTAC+AGG | + | chr8_1:49566982-49567001 | Msa1198210:intron | 65.0% | |
| GGGGGTCACTGGGACAAGGA+AGG | - | chr8_1:49567485-49567504 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 49566658 | 49567675 | 49566658 | ID=Msa1198210;Name=Msa1198210 |
| chr8_1 | mRNA | 49566658 | 49567675 | 49566658 | ID=Msa1198210-mRNA-1;Parent=Msa1198210;Name=Msa1198210-mRNA-1;_AED=0.47;_eAED=0.48;_QI=0|0|0|0.5|0|0|2|0|81 |
| chr8_1 | exon | 49566658 | 49566897 | 49566658 | ID=Msa1198210-mRNA-1:exon:15557;Parent=Msa1198210-mRNA-1 |
| chr8_1 | exon | 49567670 | 49567675 | 49567670 | ID=Msa1198210-mRNA-1:exon:15558;Parent=Msa1198210-mRNA-1 |
| chr8_1 | CDS | 49566658 | 49566897 | 49566658 | ID=Msa1198210-mRNA-1:cds;Parent=Msa1198210-mRNA-1 |
| chr8_1 | CDS | 49567670 | 49567675 | 49567670 | ID=Msa1198210-mRNA-1:cds;Parent=Msa1198210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
