AlfalfaGEDB | Msa1200800

M. sativa cultivar zhongmu-4 / Msa1200800

KEGG: K10802|K11295|K11296
GO: GO:0000785 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003700 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006325 GO:0006333 GO:0006355 GO:0006996 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0019219 GO:0019222 GO:0030527 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00505.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1200800	sp\|P40620\|HMGL_VICFA	73.846	130	28	2	3	126	5	134	6.37e-58	179

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1200800	B7FN80	99.296	142	1	0	1	142	1	142	3.43e-94	277

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TFs/TRs:

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Gene ID	Type	Classification
Msa1200800	TR	HMG

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1200800	Msa1294070	0.835379	1.736707e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1200800	MtrunA17_Chr8g0370431	99.296	142	1	0	1	142	1	142	6.61e-98	277
Msa1200800	MtrunA17_Chr5g0407011	67.969	128	38	2	1	126	1	127	5.65e-54	166
Msa1200800	MtrunA17_Chr3g0143991	64.286	112	34	3	3	108	5	116	2.86e-37	124
Msa1200800	MtrunA17_Chr7g0242251	60.606	99	38	1	33	130	47	145	1.90e-30	107
Msa1200800	MtrunA17_Chr1g0212341	44.340	106	50	1	33	138	98	194	5.37e-24	92.4
Msa1200800	MtrunA17_Chr4g0052011	50.000	78	39	0	33	110	60	137	2.00e-22	86.7
Msa1200800	MtrunA17_Chr8g0387931	45.783	83	39	2	33	115	262	338	1.28e-12	63.9
Msa1200800	MtrunA17_Chr1g0155251	43.023	86	48	1	22	107	543	627	7.06e-11	58.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1200800	AT1G20693.2	61.871	139	40	4	3	138	14	142	2.70e-44	142
Msa1200800	AT2G17560.2	56.618	136	53	2	5	138	7	138	1.18e-42	138
Msa1200800	AT2G17560.1	56.618	136	53	2	5	138	7	138	1.18e-42	138
Msa1200800	AT1G20693.3	61.600	125	37	3	3	126	14	128	2.35e-40	132
Msa1200800	AT1G20693.1	61.600	125	37	3	3	126	14	128	2.42e-40	132
Msa1200800	AT2G17560.3	56.296	135	50	3	5	137	7	134	2.77e-40	132
Msa1200800	AT1G20696.1	68.932	103	31	1	25	126	23	125	1.05e-39	130
Msa1200800	AT3G51880.2	64.423	104	33	2	28	130	44	144	2.84e-37	125
Msa1200800	AT3G51880.5	64.423	104	33	2	28	130	44	144	3.60e-37	125
Msa1200800	AT3G51880.1	64.423	104	33	2	28	130	44	144	3.60e-37	125

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Find 56 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGAAGAAAGCACTTGG+AGG	0.558317	8_1:+53747978	None:intergenic
AAAATCCGAAGTCAATGATG+AGG	0.642367	8_1:-53747252	Msa1200800:CDS
AAAGCACTTGGAGGTCTCTT+TGG	0.290758	8_1:+53747987	None:intergenic
AAAGGAGCGAGTTGCTGAAT+CGG	0.487717	8_1:+53748265	None:intergenic
AACATTGACTTTAAGTACAC+AGG	0.550744	8_1:+53746903	None:intergenic
AACGAGAGAGGGGATAAGAA+GGG	0.608282	8_1:+53748352	None:intergenic
AAGCGCAAAGGTGCTGGAGC+TGG	0.461656	8_1:-53748050	Msa1200800:CDS
AATTAGGTCGGAAAAGCTTG+CGG	0.611496	8_1:-53747658	Msa1200800:intron
ACAAGCCTACAACAAGAAAC+TGG	0.447828	8_1:-53747411	Msa1200800:intron
ACAAGGCTCCTTATGCTGCT+AGG	0.511412	8_1:-53747469	Msa1200800:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCAATCAAATAACTTCACA+CGG	-	chr8_1:53746861-53746880	Msa1200800:three_prime_UTR	30.0%
AGAGAAAACGCAAAGAAAAA+AGG	+	chr8_1:53746953-53746972	None:intergenic	30.0%
TTGGAGAAGAAAAAATCGAA+CGG	+	chr8_1:53747173-53747192	None:intergenic	30.0%
GAAGTTTAGGGAAAAATTGT+TGG	-	chr8_1:53748294-53748313	Msa1200800:five_prime_UTR	30.0%
ACCAATCAAATAACTTCACA+CGG	-	chr8_1:53746861-53746880	Msa1200800:three_prime_UTR	30.0%
AGAGAAAACGCAAAGAAAAA+AGG	+	chr8_1:53746953-53746972	None:intergenic	30.0%
TTGGAGAAGAAAAAATCGAA+CGG	+	chr8_1:53747173-53747192	None:intergenic	30.0%
GAAGTTTAGGGAAAAATTGT+TGG	-	chr8_1:53748294-53748313	Msa1200800:five_prime_UTR	30.0%
TCCGTGTGAAGTTATTTGAT+TGG	+	chr8_1:53746865-53746884	None:intergenic	35.0%
GTAAACGAGATTTCTTACAG+CGG	+	chr8_1:53747565-53747584	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	53746833	53748534	53746833	ID=Msa1200800;Name=Msa1200800
chr8_1	mRNA	53746833	53748534	53746833	ID=Msa1200800-mRNA-1;Parent=Msa1200800;Name=Msa1200800-mRNA-1;_AED=0.06;_eAED=0.06;_QI=281\|1\|1\|1\|1\|1\|7\|274\|142
chr8_1	exon	53746833	53747139	53746833	ID=Msa1200800-mRNA-1:exon:17238;Parent=Msa1200800-mRNA-1
chr8_1	exon	53747241	53747309	53747241	ID=Msa1200800-mRNA-1:exon:17237;Parent=Msa1200800-mRNA-1
chr8_1	exon	53747412	53747492	53747412	ID=Msa1200800-mRNA-1:exon:17236;Parent=Msa1200800-mRNA-1
chr8_1	exon	53747630	53747674	53747630	ID=Msa1200800-mRNA-1:exon:17235;Parent=Msa1200800-mRNA-1
chr8_1	exon	53747801	53747858	53747801	ID=Msa1200800-mRNA-1:exon:17234;Parent=Msa1200800-mRNA-1
chr8_1	exon	53747975	53748076	53747975	ID=Msa1200800-mRNA-1:exon:17233;Parent=Msa1200800-mRNA-1
chr8_1	exon	53748213	53748534	53748213	ID=Msa1200800-mRNA-1:exon:17232;Parent=Msa1200800-mRNA-1
chr8_1	five_prime_UTR	53748254	53748534	53748254	ID=Msa1200800-mRNA-1:five_prime_utr;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53748213	53748253	53748213	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747975	53748076	53747975	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747801	53747858	53747801	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747630	53747674	53747630	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747412	53747492	53747412	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747241	53747309	53747241	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	CDS	53747107	53747139	53747107	ID=Msa1200800-mRNA-1:cds;Parent=Msa1200800-mRNA-1
chr8_1	three_prime_UTR	53746833	53747106	53746833	ID=Msa1200800-mRNA-1:three_prime_utr;Parent=Msa1200800-mRNA-1

Gene Sequence

>Msa1200800

ATGCCGAAAGTTAAAGCTGATTCCAAACCCGCTGATAACCGCTTGAAGCGCAAAGGTGCTGGAGCTGGAAGGAAACAGACGAAGAAAGCAGCGAAGGATCCTAACAAGCCAAAGAGACCTCCAAGTGCTTTCTTCGTTTTCATGTCCGATTTTAGAGAACAGTATAAGAAAGATCATCCTAACAACAAATCCGTTGCCGCTGTCGGAAAAGCTTGCGGTGAAGCATGGAAATCGATGTCTGAAGAAGACAAGGCTCCTTATGCTGCTAGGGCATTGAAGAAGAAAGAAGAGTATGAAGTTGCTACACAAGCCTACAACAAGAAACTGGAGGGAAAAGACGAGGAAGATGGTTCTGACAAGTCAAAATCCGAAGTCAATGATGAGGATGAAGATGAGGAGGATGAGGATGATGATGAAGATGATGACTGA

Protein sequence

>Msa1200800

MPKVKADSKPADNRLKRKGAGAGRKQTKKAAKDPNKPKRPPSAFFVFMSDFREQYKKDHPNNKSVAAVGKACGEAWKSMSEEDKAPYAARALKKKEEYEVATQAYNKKLEGKDEEDGSDKSKSEVNDEDEDEEDEDDDEDDD*