AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1200850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200850 MtrunA17_Chr8g0370491 97.935 339 6 1 129 466 22 360 0.0 689
Msa1200850 MtrunA17_Chr1g0199941 90.855 339 30 1 129 466 22 360 0.0 640
Msa1200850 MtrunA17_Chr5g0429771 32.704 159 100 2 135 293 73 224 2.42e-20 91.7
Msa1200850 MtrunA17_Chr5g0429791 42.029 69 39 1 140 208 81 148 6.82e-12 63.9
Msa1200850 MtrunA17_Chr5g0429781 41.791 67 39 0 390 456 68 134 2.16e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1200850 AT2G17570.1 48.817 338 103 5 129 466 28 295 2.86e-102 307
Msa1200850 AT5G60510.1 45.556 180 97 1 126 304 21 200 5.26e-48 166
Msa1200850 AT5G60510.1 55.696 79 35 0 386 464 192 270 1.90e-23 100
Msa1200850 AT5G60500.1 46.341 164 87 1 126 288 20 183 4.34e-45 159
Msa1200850 AT5G60500.1 52.381 84 40 0 381 464 186 269 6.45e-23 98.6
Msa1200850 AT5G58770.1 31.915 141 93 1 140 280 77 214 4.48e-18 85.1
Msa1200850 AT5G58770.1 37.234 94 54 2 365 456 212 302 3.24e-11 64.7
Msa1200850 AT5G58784.1 29.870 154 101 2 140 293 70 216 4.68e-18 85.1
Msa1200850 AT5G58784.1 57.143 49 17 1 403 447 242 290 3.21e-11 64.7
Msa1200850 AT2G23410.1 31.544 149 96 2 140 286 70 214 6.36e-18 84.7
Msa1200850 AT5G58780.1 30.612 147 99 1 140 286 70 213 1.24e-17 84.0
Msa1200850 AT5G58782.1 29.932 147 100 1 140 286 57 200 1.78e-16 80.1
Msa1200850 AT5G58782.2 29.932 147 100 1 140 286 75 218 2.39e-16 80.1
Msa1200850 AT2G23400.2 36.290 124 70 3 140 257 70 190 3.79e-16 77.4
Msa1200850 AT2G23400.1 35.514 107 66 1 140 246 70 173 2.35e-15 77.0

Find 104 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAGCATCGCAAACTGTAAA+AGG 0.229597 8_1:-53821465 None:intergenic
ATGAATGTTTGAACCTCTTT+AGG 0.235814 8_1:-53822143 None:intergenic
CACGATCCATGTAAAGAAAA+TGG 0.242050 8_1:+53822503 Msa1200850:CDS
CCTTGAATCGAAACAACTTT+TGG 0.252546 8_1:+53820558 Msa1200850:CDS
CCAAAAGTTGTTTCGATTCA+AGG 0.264002 8_1:-53820558 None:intergenic
CCTTAGATACTGTTATGAAT+TGG 0.280108 8_1:+53822067 Msa1200850:CDS
AAGAATCTTGCAAGGATAAA+TGG 0.283471 8_1:+53822384 Msa1200850:CDS
ATTTCAGGCCAAAGTACTTT+TGG 0.326293 8_1:-53822836 None:intergenic
AAAGTACTTTGGCCTGAAAT+TGG 0.327946 8_1:+53822839 Msa1200850:CDS
TGAAATTACAAGAATAAATC+AGG 0.333383 8_1:+53822448 Msa1200850:CDS
CCAATTCATAACAGTATCTA+AGG 0.340714 8_1:-53822067 None:intergenic
GACGTTCTGATCAAGATATC+TGG 0.340934 8_1:-53822752 None:intergenic
GGTGTTAGATTACATTTCAT+TGG 0.348743 8_1:+53822227 Msa1200850:CDS
CCATATCTGTATGTGCTTCA+TGG 0.356260 8_1:+53820481 Msa1200850:CDS
TTCATGATTACTGCAAGCTT+TGG 0.370650 8_1:-53822528 None:intergenic
AAAAGTGCAGGAAGTATTAC+AGG 0.371310 8_1:+53821867 Msa1200850:CDS
CTTAGATACTGTTATGAATT+GGG 0.374432 8_1:+53822068 Msa1200850:CDS
AAGTGTCTCAGACCAATTTC+AGG 0.406669 8_1:-53822851 None:intergenic
AGTCGTGTGGGGTGGTGGCT+TGG 0.408843 8_1:+53820625 Msa1200850:CDS
CCATGAAGCACATACAGATA+TGG 0.431029 8_1:-53820481 None:intergenic
ATCAATGCTGAATGCATAAA+CGG 0.436596 8_1:-53822105 None:intergenic
GATCGAGGAGTTGCTTCAAC+AGG 0.436725 8_1:+53822184 Msa1200850:CDS
AAAGAACTCTCTCTTGGTTG+TGG 0.443443 8_1:-53822313 None:intergenic
CATATTGCTTTCATCATGGA+TGG 0.445076 8_1:+53821966 Msa1200850:CDS
CAAAGGTCCGGCTGATAAAA+CGG 0.448720 8_1:-53821937 None:intergenic
TGCAGCAATCCTGACAGGTT+CGG 0.456152 8_1:-53822267 None:intergenic
AGTATTACAGGCCACTTGCT+TGG 0.458501 8_1:+53821879 Msa1200850:CDS
TGTCAGGATTGCTGCAGAAA+AGG 0.460884 8_1:+53822274 Msa1200850:CDS
GGGAGAAGGGGATGGTCATA+AGG 0.461239 8_1:+53822019 Msa1200850:CDS
GTGAAATCACATCTTGTAAC+GGG 0.464158 8_1:+53822627 Msa1200850:CDS
AAGCATCGCAAACTGTAAAA+GGG 0.465563 8_1:-53821464 None:intergenic
AACTATTGACCGAACCTGTC+AGG 0.468521 8_1:+53822258 Msa1200850:CDS
AACATTCATGGAATTGATGC+GGG 0.468870 8_1:+53822157 Msa1200850:CDS
TGTATGCACCAAAAGTACTT+TGG 0.470623 8_1:+53822828 Msa1200850:CDS
AAGAACTCTCTCTTGGTTGT+GGG 0.473682 8_1:-53822312 None:intergenic
TAAAGAGGTTCAAACATTCA+TGG 0.476162 8_1:+53822145 Msa1200850:CDS
GTACGCAAGGAGGAGAAACT+TGG 0.478320 8_1:+53821998 Msa1200850:CDS
CATGAAGAAGGGGAAGGTGC+TGG 0.479786 8_1:+53822545 Msa1200850:CDS
CGTGTGGGGTGGTGGCTTGG+TGG 0.481557 8_1:+53820628 Msa1200850:CDS
AAATTAAAAGAACTCTCTCT+TGG 0.485452 8_1:-53822319 None:intergenic
GAATCATATTGCTTTCATCA+TGG 0.485927 8_1:+53821962 Msa1200850:CDS
ATGATGAAAGCAATATGATT+CGG 0.490273 8_1:-53821960 None:intergenic
GGAGGAGAAACTTGGGAGAA+GGG 0.496488 8_1:+53822006 Msa1200850:CDS
CTGTCAGAAATGAAACTTGA+AGG 0.498611 8_1:-53821832 None:intergenic
CCACCCCACACGACTAAAAG+AGG 0.504272 8_1:-53820617 None:intergenic
ATTACAGGCCACTTGCTTGG+AGG 0.512105 8_1:+53821882 Msa1200850:CDS
GCTTGCAGTAATCATGAAGA+AGG 0.515262 8_1:+53822533 Msa1200850:CDS
AATATGATTCGGCAAAGGTC+CGG 0.517381 8_1:-53821949 None:intergenic
GAAAGCATTATTAATGAATA+CGG 0.521539 8_1:+53822206 Msa1200850:CDS
CTGATCAAGATATCTGGGTC+AGG 0.521825 8_1:-53822746 None:intergenic
ATGGACAAGTTGGGATGGGA+TGG 0.521944 8_1:+53820500 Msa1200850:CDS
TTGGTCTGGATTGTCGGGAG+AGG 0.522305 8_1:+53820577 Msa1200850:CDS
CTCCCGATGCAGAAAAGTGC+AGG 0.528709 8_1:+53821855 Msa1200850:CDS
GATATCTTGATCAGAACGTC+GGG 0.528895 8_1:+53822755 Msa1200850:CDS
GCTTTATTGCAGCAAACTGA+TGG 0.530253 8_1:+53821408 Msa1200850:intron
GAAGGGGATGGTCATAAGGC+TGG 0.530762 8_1:+53822023 Msa1200850:CDS
ATGTGCTTCATGGACAAGTT+GGG 0.534009 8_1:+53820491 Msa1200850:CDS
TTGAAGAGGAATCTTAGCAC+TGG 0.535461 8_1:-53820437 None:intergenic
GCAGCAAACTGATGGAAGAA+AGG 0.539017 8_1:+53821416 Msa1200850:intron
CTTGATCAGAACGTCGGGAG+AGG 0.549689 8_1:+53822760 Msa1200850:CDS
CTTCAGAAGGAAGCCTAAAG+AGG 0.551680 8_1:+53822130 Msa1200850:CDS
AGTAGCGATAATTGCAGTGA+AGG 0.553721 8_1:+53822605 Msa1200850:CDS
AATCAGGGCCCGAAAAGAAA+CGG 0.557633 8_1:+53822464 Msa1200850:CDS
GAGGAGAAACTTGGGAGAAG+GGG 0.558845 8_1:+53822007 Msa1200850:CDS
AGAAACTTGGGAGAAGGGGA+TGG 0.560004 8_1:+53822011 Msa1200850:CDS
TATGTGCTTCATGGACAAGT+TGG 0.561006 8_1:+53820490 Msa1200850:CDS
ACGTTCTGATCAAGATATCT+GGG 0.563021 8_1:-53822751 None:intergenic
AGGAGGAGAAACTTGGGAGA+AGG 0.564524 8_1:+53822005 Msa1200850:CDS
CTTGCAGTAATCATGAAGAA+GGG 0.580142 8_1:+53822534 Msa1200850:CDS
TTAGCACTGGATGGTTGTTG+AGG 0.583923 8_1:-53820424 None:intergenic
ATATTGCTTTCATCATGGAT+GGG 0.586307 8_1:+53821967 Msa1200850:CDS
ATTCGAAACTTTATCTGACG+TGG 0.588225 8_1:-53822423 None:intergenic
TGAAATTGGTCTGAGACACT+TGG 0.588657 8_1:+53822853 Msa1200850:CDS
AATATAAACCTCCAAGCAAG+TGG 0.593519 8_1:-53821890 None:intergenic
AACTCTCTCTTGGTTGTGGG+CGG 0.593794 8_1:-53822309 None:intergenic
AAACATTCATGGAATTGATG+CGG 0.594151 8_1:+53822156 Msa1200850:CDS
GAAACTTTATCTGACGTGGA+TGG 0.594282 8_1:-53822419 None:intergenic
AGAGGAATCTTAGCACTGGA+TGG 0.596482 8_1:-53820433 None:intergenic
TCAGCATTGATAACTTCAGA+AGG 0.597370 8_1:+53822117 Msa1200850:CDS
AGCATCGCAAACTGTAAAAG+GGG 0.599673 8_1:-53821463 None:intergenic
ACACGACACTGAGTTTGAAG+AGG 0.606081 8_1:-53820451 None:intergenic
CTTCATGGACAAGTTGGGAT+GGG 0.611149 8_1:+53820496 Msa1200850:CDS
TGTCAGAAATGAAACTTGAA+GGG 0.614770 8_1:-53821831 None:intergenic
AAAGGCAGCTAAAGTGCTCA+CGG 0.615660 8_1:+53821434 Msa1200850:CDS
GAAATTACAAGAATAAATCA+GGG 0.617698 8_1:+53822449 Msa1200850:CDS
TTGCAGTAATCATGAAGAAG+GGG 0.619261 8_1:+53822535 Msa1200850:CDS
TGCTGTTCAAGAATCTTGCA+AGG 0.626589 8_1:+53822376 Msa1200850:CDS
GGTGAAATCACATCTTGTAA+CGG 0.629874 8_1:+53822626 Msa1200850:CDS
ACGTCAGATAAAGTTTCGAA+TGG 0.632374 8_1:+53822425 Msa1200850:CDS
GCTTCATGGACAAGTTGGGA+TGG 0.632987 8_1:+53820495 Msa1200850:CDS
TACGCAAGGAGGAGAAACTT+GGG 0.634257 8_1:+53821999 Msa1200850:CDS
TCATCATGGATGGGAATCGA+AGG 0.636036 8_1:+53821976 Msa1200850:CDS
AGATATCTTGATCAGAACGT+CGG 0.637734 8_1:+53822754 Msa1200850:CDS
ATGGGAATCGAAGGTACGCA+AGG 0.640679 8_1:+53821985 Msa1200850:CDS
TGAAGAGCAAAAGTACAACC+AGG 0.650657 8_1:+53822664 Msa1200850:CDS
AAAGCAATATGATTCGGCAA+AGG 0.662745 8_1:-53821954 None:intergenic
TGGTCTGAGACACTTGGTGT+GGG 0.663899 8_1:+53822859 Msa1200850:CDS
GGAATCGAAGGTACGCAAGG+AGG 0.664908 8_1:+53821988 Msa1200850:CDS
TTATAGAAGCAATCTCACCC+TGG 0.666787 8_1:-53822682 None:intergenic
TTGGTCTGAGACACTTGGTG+TGG 0.674555 8_1:+53822858 Msa1200850:CDS
AGTAATCATGAAGAAGGGGA+AGG 0.677452 8_1:+53822539 Msa1200850:CDS
ATTGATGCGGGAAAAGATCG+AGG 0.677470 8_1:+53822169 Msa1200850:CDS
GAAGAGCAAAAGTACAACCA+GGG 0.717793 8_1:+53822665 Msa1200850:CDS
GAAGCACATGTATATGACAG+TGG 0.724422 8_1:+53822721 Msa1200850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCAAAAGAAAAAATGA+AGG - chr8_1:53820151-53820170 None:intergenic 15.0%
!! ATATAAATCAAAGAATCAAA+AGG - chr8_1:53821088-53821107 None:intergenic 15.0%
!!! AAAAATTATCATTTGTTGAT+TGG + chr8_1:53821498-53821517 Msa1200850:intron 15.0%
!!! TGTGTATATGTATATTTTTT+CGG + chr8_1:53821705-53821724 Msa1200850:intron 15.0%
!!! AAAGAAGAGACAAATTTTAT+CGG - chr8_1:53820117-53820136 None:intergenic 20.0%
!! AAGAAAAATCATTTGTTCAA+TGG + chr8_1:53821671-53821690 Msa1200850:intron 20.0%
!!! GAAAGCATTATTAATGAATA+CGG + chr8_1:53822206-53822225 Msa1200850:CDS 20.0%
!! TGAAATTACAAGAATAAATC+AGG + chr8_1:53822448-53822467 Msa1200850:CDS 20.0%
!! GAAATTACAAGAATAAATCA+GGG + chr8_1:53822449-53822468 Msa1200850:CDS 20.0%
!!! TTTTCTTTTCCAAGTAAAAA+TGG - chr8_1:53822907-53822926 None:intergenic 20.0%
! AAATTTGTCTCTTCTTTCAA+AGG + chr8_1:53820120-53820139 Msa1200850:intron 25.0%
! TACTAAAGAACAACAAGAAT+TGG - chr8_1:53820175-53820194 None:intergenic 25.0%
! TTCATACTGTATTCACTTAA+TGG + chr8_1:53820286-53820305 Msa1200850:intron 25.0%
! TAAGTTTGATTGAGCATAAT+AGG + chr8_1:53820389-53820408 Msa1200850:intron 25.0%
!!! GATGGTTTTGTTGTTAATTT+TGG + chr8_1:53820518-53820537 Msa1200850:CDS 25.0%
! TATGCATTATAGCTTCAATT+TGG + chr8_1:53820931-53820950 Msa1200850:intron 25.0%
! TATCATTGAAGTATATTCAG+AGG + chr8_1:53820970-53820989 Msa1200850:intron 25.0%
! TTTCATGATCTTTCTCTTTA+TGG + chr8_1:53821050-53821069 Msa1200850:intron 25.0%
! TATCATTGAAGTATATTCAG+AGG + chr8_1:53820970-53820989 Msa1200850:intron 25.0%
! TTTCATGATCTTTCTCTTTA+TGG + chr8_1:53821050-53821069 Msa1200850:intron 25.0%
! AGCAAAATCAAAGAATCAAA+AGG - chr8_1:53821230-53821249 None:intergenic 25.0%
! ATGAAATATTCTTCTCATAC+AGG + chr8_1:53821796-53821815 Msa1200850:intron 25.0%
! ATGATGAAAGCAATATGATT+CGG - chr8_1:53821963-53821982 None:intergenic 25.0%
! CTTAGATACTGTTATGAATT+GGG + chr8_1:53822068-53822087 Msa1200850:CDS 25.0%
! AAATTAAAAGAACTCTCTCT+TGG - chr8_1:53822322-53822341 None:intergenic 25.0%
!! AAATTAAGACCGTTTCTTTT+CGG - chr8_1:53822476-53822495 None:intergenic 25.0%
!!! TTATTTTGATTACGCTGCTT+AGG + chr8_1:53820225-53820244 Msa1200850:intron 30.0%
! CTGAAATGAAGCATTTCAAA+TGG + chr8_1:53820309-53820328 Msa1200850:intron 30.0%
! CTATATTGATGACATGTAAG+TGG + chr8_1:53820745-53820764 Msa1200850:intron 30.0%
AAGTGGTAGTAATTAGTTGT+AGG + chr8_1:53820762-53820781 Msa1200850:intron 30.0%
AAAGAGAAAGATCATGAAAG+AGG - chr8_1:53821049-53821068 None:intergenic 30.0%
AAAGAGAAAGATCATGAAAG+AGG - chr8_1:53821049-53821068 None:intergenic 30.0%
TGTCAGAAATGAAACTTGAA+GGG - chr8_1:53821834-53821853 None:intergenic 30.0%
GAATCATATTGCTTTCATCA+TGG + chr8_1:53821962-53821981 Msa1200850:CDS 30.0%
ATATTGCTTTCATCATGGAT+GGG + chr8_1:53821967-53821986 Msa1200850:CDS 30.0%
CCAATTCATAACAGTATCTA+AGG - chr8_1:53822070-53822089 None:intergenic 30.0%
CCTTAGATACTGTTATGAAT+TGG + chr8_1:53822067-53822086 Msa1200850:CDS 30.0%
ATCAATGCTGAATGCATAAA+CGG - chr8_1:53822108-53822127 None:intergenic 30.0%
ATGAATGTTTGAACCTCTTT+AGG - chr8_1:53822146-53822165 None:intergenic 30.0%
TAAAGAGGTTCAAACATTCA+TGG + chr8_1:53822145-53822164 Msa1200850:CDS 30.0%
AAACATTCATGGAATTGATG+CGG + chr8_1:53822156-53822175 Msa1200850:CDS 30.0%
GGTGTTAGATTACATTTCAT+TGG + chr8_1:53822227-53822246 Msa1200850:CDS 30.0%
!!! GAGAGTTCTTTTAATTTGTG+TGG + chr8_1:53822325-53822344 Msa1200850:CDS 30.0%
AAGAATCTTGCAAGGATAAA+TGG + chr8_1:53822384-53822403 Msa1200850:CDS 30.0%
! AATTAAGACCGTTTCTTTTC+GGG - chr8_1:53822475-53822494 None:intergenic 30.0%
! TTGGTTCCATTTTCTTTACA+TGG - chr8_1:53822512-53822531 None:intergenic 30.0%
! GTCTCAGTAATTTTCTTCTT+TGG + chr8_1:53822786-53822805 Msa1200850:CDS 30.0%
! TTTGAGATTTGGTTTCAACG+AGG + chr8_1:53820070-53820089 Msa1200850:intron 35.0%
! CCAAAAGTTGTTTCGATTCA+AGG - chr8_1:53820561-53820580 None:intergenic 35.0%
! CCTTGAATCGAAACAACTTT+TGG + chr8_1:53820558-53820577 Msa1200850:CDS 35.0%
! AATCGAAACAACTTTTGGTC+TGG + chr8_1:53820563-53820582 Msa1200850:CDS 35.0%
! TGTGCTTACTCTAAAAGTTC+AGG + chr8_1:53820803-53820822 Msa1200850:intron 35.0%
CAAAGCTCAGATATTGATCA+AGG - chr8_1:53821147-53821166 None:intergenic 35.0%
!! TCTATTTTTCATCGAGCGTA+GGG - chr8_1:53821369-53821388 None:intergenic 35.0%
! AAGCATCGCAAACTGTAAAA+GGG - chr8_1:53821467-53821486 None:intergenic 35.0%
! TAAGCATCGCAAACTGTAAA+AGG - chr8_1:53821468-53821487 None:intergenic 35.0%
AGCATCACAAACTGTAAAAC+TGG - chr8_1:53821640-53821659 None:intergenic 35.0%
CTGTCAGAAATGAAACTTGA+AGG - chr8_1:53821835-53821854 None:intergenic 35.0%
!! AAAAGTGCAGGAAGTATTAC+AGG + chr8_1:53821867-53821886 Msa1200850:CDS 35.0%
AATATAAACCTCCAAGCAAG+TGG - chr8_1:53821893-53821912 None:intergenic 35.0%
! AAAGCAATATGATTCGGCAA+AGG - chr8_1:53821957-53821976 None:intergenic 35.0%
CATATTGCTTTCATCATGGA+TGG + chr8_1:53821966-53821985 Msa1200850:CDS 35.0%
!! TCAGCATTGATAACTTCAGA+AGG + chr8_1:53822117-53822136 Msa1200850:CDS 35.0%
AACATTCATGGAATTGATGC+GGG + chr8_1:53822157-53822176 Msa1200850:CDS 35.0%
ATTCGAAACTTTATCTGACG+TGG - chr8_1:53822426-53822445 None:intergenic 35.0%
ACGTCAGATAAAGTTTCGAA+TGG + chr8_1:53822425-53822444 Msa1200850:CDS 35.0%
CACGATCCATGTAAAGAAAA+TGG + chr8_1:53822503-53822522 Msa1200850:CDS 35.0%
TTCATGATTACTGCAAGCTT+TGG - chr8_1:53822531-53822550 None:intergenic 35.0%
CTTGCAGTAATCATGAAGAA+GGG + chr8_1:53822534-53822553 Msa1200850:CDS 35.0%
TTGCAGTAATCATGAAGAAG+GGG + chr8_1:53822535-53822554 Msa1200850:CDS 35.0%
GGTGAAATCACATCTTGTAA+CGG + chr8_1:53822626-53822645 Msa1200850:CDS 35.0%
GTGAAATCACATCTTGTAAC+GGG + chr8_1:53822627-53822646 Msa1200850:CDS 35.0%
ACGTTCTGATCAAGATATCT+GGG - chr8_1:53822754-53822773 None:intergenic 35.0%
AGATATCTTGATCAGAACGT+CGG + chr8_1:53822754-53822773 Msa1200850:CDS 35.0%
!! GTACTTTTGGTGCATACAAA+GGG - chr8_1:53822826-53822845 None:intergenic 35.0%
!! AGTACTTTTGGTGCATACAA+AGG - chr8_1:53822827-53822846 None:intergenic 35.0%
!! TGTATGCACCAAAAGTACTT+TGG + chr8_1:53822828-53822847 Msa1200850:CDS 35.0%
!! ATTTCAGGCCAAAGTACTTT+TGG - chr8_1:53822839-53822858 None:intergenic 35.0%
! AAAGTACTTTGGCCTGAAAT+TGG + chr8_1:53822839-53822858 Msa1200850:CDS 35.0%
!! TCAGAGACACCATTTTTACT+TGG + chr8_1:53822895-53822914 Msa1200850:CDS 35.0%
TTGAAGAGGAATCTTAGCAC+TGG - chr8_1:53820440-53820459 None:intergenic 40.0%
CCATGAAGCACATACAGATA+TGG - chr8_1:53820484-53820503 None:intergenic 40.0%
CCATATCTGTATGTGCTTCA+TGG + chr8_1:53820481-53820500 Msa1200850:CDS 40.0%
TATGTGCTTCATGGACAAGT+TGG + chr8_1:53820490-53820509 Msa1200850:CDS 40.0%
ATGTGCTTCATGGACAAGTT+GGG + chr8_1:53820491-53820510 Msa1200850:CDS 40.0%
! CAACTTTTGGTCTGGATTGT+CGG + chr8_1:53820571-53820590 Msa1200850:CDS 40.0%
! AACTTTTGGTCTGGATTGTC+GGG + chr8_1:53820572-53820591 Msa1200850:CDS 40.0%
! TAGAACCTCTTTTAGTCGTG+TGG + chr8_1:53820612-53820631 Msa1200850:CDS 40.0%
! AGAACCTCTTTTAGTCGTGT+GGG + chr8_1:53820613-53820632 Msa1200850:CDS 40.0%
TTGTAGTGTTTGCTCTTTGC+TGG + chr8_1:53820665-53820684 Msa1200850:intron 40.0%
GAGTAAGCACAGTCAATACA+TGG - chr8_1:53820794-53820813 None:intergenic 40.0%
CAATATCTGAGCTTTGCTCT+AGG + chr8_1:53821151-53821170 Msa1200850:intron 40.0%
!! GTCTATTTTTCATCGAGCGT+AGG - chr8_1:53821370-53821389 None:intergenic 40.0%
! GCTTTATTGCAGCAAACTGA+TGG + chr8_1:53821408-53821427 Msa1200850:intron 40.0%
AGCATCGCAAACTGTAAAAG+GGG - chr8_1:53821466-53821485 None:intergenic 40.0%
!!! ATCTTTGCCGTTTTATCAGC+CGG + chr8_1:53821930-53821949 Msa1200850:CDS 40.0%
! AATATGATTCGGCAAAGGTC+CGG - chr8_1:53821952-53821971 None:intergenic 40.0%
AAGAACTCTCTCTTGGTTGT+GGG - chr8_1:53822315-53822334 None:intergenic 40.0%
AAAGAACTCTCTCTTGGTTG+TGG - chr8_1:53822316-53822335 None:intergenic 40.0%
TGCTGTTCAAGAATCTTGCA+AGG + chr8_1:53822376-53822395 Msa1200850:CDS 40.0%
GAAACTTTATCTGACGTGGA+TGG - chr8_1:53822422-53822441 None:intergenic 40.0%
GCTTGCAGTAATCATGAAGA+AGG + chr8_1:53822533-53822552 Msa1200850:CDS 40.0%
AGTAATCATGAAGAAGGGGA+AGG + chr8_1:53822539-53822558 Msa1200850:CDS 40.0%
AGTAGCGATAATTGCAGTGA+AGG + chr8_1:53822605-53822624 Msa1200850:CDS 40.0%
! TGAAGAGCAAAAGTACAACC+AGG + chr8_1:53822664-53822683 Msa1200850:CDS 40.0%
! GAAGAGCAAAAGTACAACCA+GGG + chr8_1:53822665-53822684 Msa1200850:CDS 40.0%
TTATAGAAGCAATCTCACCC+TGG - chr8_1:53822685-53822704 None:intergenic 40.0%
GAAGCACATGTATATGACAG+TGG + chr8_1:53822721-53822740 Msa1200850:CDS 40.0%
GACGTTCTGATCAAGATATC+TGG - chr8_1:53822755-53822774 None:intergenic 40.0%
GATATCTTGATCAGAACGTC+GGG + chr8_1:53822755-53822774 Msa1200850:CDS 40.0%
AAGTGTCTCAGACCAATTTC+AGG - chr8_1:53822854-53822873 None:intergenic 40.0%
! TGAAATTGGTCTGAGACACT+TGG + chr8_1:53822853-53822872 Msa1200850:CDS 40.0%
TATCGGACGAGAACTAACGT+TGG - chr8_1:53820100-53820119 None:intergenic 45.0%
! TTAGCACTGGATGGTTGTTG+AGG - chr8_1:53820427-53820446 None:intergenic 45.0%
! AGAGGAATCTTAGCACTGGA+TGG - chr8_1:53820436-53820455 None:intergenic 45.0%
ACACGACACTGAGTTTGAAG+AGG - chr8_1:53820454-53820473 None:intergenic 45.0%
! CTTCATGGACAAGTTGGGAT+GGG + chr8_1:53820496-53820515 Msa1200850:CDS 45.0%
! GAACCTCTTTTAGTCGTGTG+GGG + chr8_1:53820614-53820633 Msa1200850:CDS 45.0%
GCAAGCTCAGACATTGATCA+AGG - chr8_1:53821006-53821025 None:intergenic 45.0%
! TCAATGTCTGAGCTTGCTCT+AGG + chr8_1:53821009-53821028 Msa1200850:intron 45.0%
GCAGCAAACTGATGGAAGAA+AGG + chr8_1:53821416-53821435 Msa1200850:intron 45.0%
! AAAGGCAGCTAAAGTGCTCA+CGG + chr8_1:53821434-53821453 Msa1200850:CDS 45.0%
GCTGCAAACTGACGTAAGAA+AGG + chr8_1:53821590-53821609 Msa1200850:intron 45.0%
! TTCCTGCACTTTTCTGCATC+GGG - chr8_1:53821860-53821879 None:intergenic 45.0%
! CTTCCTGCACTTTTCTGCAT+CGG - chr8_1:53821861-53821880 None:intergenic 45.0%
AGTATTACAGGCCACTTGCT+TGG + chr8_1:53821879-53821898 Msa1200850:CDS 45.0%
CAAAGGTCCGGCTGATAAAA+CGG - chr8_1:53821940-53821959 None:intergenic 45.0%
TCATCATGGATGGGAATCGA+AGG + chr8_1:53821976-53821995 Msa1200850:CDS 45.0%
TACGCAAGGAGGAGAAACTT+GGG + chr8_1:53821999-53822018 Msa1200850:CDS 45.0%
CTTCAGAAGGAAGCCTAAAG+AGG + chr8_1:53822130-53822149 Msa1200850:CDS 45.0%
ATTGATGCGGGAAAAGATCG+AGG + chr8_1:53822169-53822188 Msa1200850:CDS 45.0%
AACTATTGACCGAACCTGTC+AGG + chr8_1:53822258-53822277 Msa1200850:CDS 45.0%
! TTTTCTGCAGCAATCCTGAC+AGG - chr8_1:53822275-53822294 None:intergenic 45.0%
TGTCAGGATTGCTGCAGAAA+AGG + chr8_1:53822274-53822293 Msa1200850:CDS 45.0%
AATCAGGGCCCGAAAAGAAA+CGG + chr8_1:53822464-53822483 Msa1200850:CDS 45.0%
CTGATCAAGATATCTGGGTC+AGG - chr8_1:53822749-53822768 None:intergenic 45.0%
! GCTTCATGGACAAGTTGGGA+TGG + chr8_1:53820495-53820514 Msa1200850:CDS 50.0%
! ATGGACAAGTTGGGATGGGA+TGG + chr8_1:53820500-53820519 Msa1200850:CDS 50.0%
ATTACAGGCCACTTGCTTGG+AGG + chr8_1:53821882-53821901 Msa1200850:CDS 50.0%
! ATGGGAATCGAAGGTACGCA+AGG + chr8_1:53821985-53822004 Msa1200850:CDS 50.0%
GTACGCAAGGAGGAGAAACT+TGG + chr8_1:53821998-53822017 Msa1200850:CDS 50.0%
AGGAGGAGAAACTTGGGAGA+AGG + chr8_1:53822005-53822024 Msa1200850:CDS 50.0%
GGAGGAGAAACTTGGGAGAA+GGG + chr8_1:53822006-53822025 Msa1200850:CDS 50.0%
GAGGAGAAACTTGGGAGAAG+GGG + chr8_1:53822007-53822026 Msa1200850:CDS 50.0%
AGAAACTTGGGAGAAGGGGA+TGG + chr8_1:53822011-53822030 Msa1200850:CDS 50.0%
GATCGAGGAGTTGCTTCAAC+AGG + chr8_1:53822184-53822203 Msa1200850:CDS 50.0%
TGCAGCAATCCTGACAGGTT+CGG - chr8_1:53822270-53822289 None:intergenic 50.0%
AACTCTCTCTTGGTTGTGGG+CGG - chr8_1:53822312-53822331 None:intergenic 50.0%
! TTGGTCTGAGACACTTGGTG+TGG + chr8_1:53822858-53822877 Msa1200850:CDS 50.0%
!! TGGTCTGAGACACTTGGTGT+GGG + chr8_1:53822859-53822878 Msa1200850:CDS 50.0%
TTGGTCTGGATTGTCGGGAG+AGG + chr8_1:53820577-53820596 Msa1200850:CDS 55.0%
CCACCCCACACGACTAAAAG+AGG - chr8_1:53820620-53820639 None:intergenic 55.0%
! CCTCTTTTAGTCGTGTGGGG+TGG + chr8_1:53820617-53820636 Msa1200850:CDS 55.0%
! CTTTTAGTCGTGTGGGGTGG+TGG + chr8_1:53820620-53820639 Msa1200850:CDS 55.0%
TTCATCGAGCGTAGGGACGA+CGG - chr8_1:53821362-53821381 None:intergenic 55.0%
! CTCCCGATGCAGAAAAGTGC+AGG + chr8_1:53821855-53821874 Msa1200850:CDS 55.0%
! GGAATCGAAGGTACGCAAGG+AGG + chr8_1:53821988-53822007 Msa1200850:CDS 55.0%
GGGAGAAGGGGATGGTCATA+AGG + chr8_1:53822019-53822038 Msa1200850:CDS 55.0%
GAAGGGGATGGTCATAAGGC+TGG + chr8_1:53822023-53822042 Msa1200850:CDS 55.0%
! CATGAAGAAGGGGAAGGTGC+TGG + chr8_1:53822545-53822564 Msa1200850:CDS 55.0%
CTTGATCAGAACGTCGGGAG+AGG + chr8_1:53822760-53822779 Msa1200850:CDS 55.0%
! AGTCGTGTGGGGTGGTGGCT+TGG + chr8_1:53820625-53820644 Msa1200850:CDS 65.0%
! CGTGTGGGGTGGTGGCTTGG+TGG + chr8_1:53820628-53820647 Msa1200850:CDS 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 53820062 53822940 53820062 ID=Msa1200850;Name=Msa1200850
chr8_1 mRNA 53820062 53822940 53820062 ID=Msa1200850-mRNA-1;Parent=Msa1200850;Name=Msa1200850-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|0.6|1|1|5|0|466
chr8_1 exon 53820062 53820068 53820062 ID=Msa1200850-mRNA-1:exon:17261;Parent=Msa1200850-mRNA-1
chr8_1 exon 53820411 53820649 53820411 ID=Msa1200850-mRNA-1:exon:17262;Parent=Msa1200850-mRNA-1
chr8_1 exon 53821420 53821473 53821420 ID=Msa1200850-mRNA-1:exon:17263;Parent=Msa1200850-mRNA-1
chr8_1 exon 53821818 53821903 53821818 ID=Msa1200850-mRNA-1:exon:17264;Parent=Msa1200850-mRNA-1
chr8_1 exon 53821926 53822940 53821926 ID=Msa1200850-mRNA-1:exon:17265;Parent=Msa1200850-mRNA-1
chr8_1 CDS 53820062 53820068 53820062 ID=Msa1200850-mRNA-1:cds;Parent=Msa1200850-mRNA-1
chr8_1 CDS 53820411 53820649 53820411 ID=Msa1200850-mRNA-1:cds;Parent=Msa1200850-mRNA-1
chr8_1 CDS 53821420 53821473 53821420 ID=Msa1200850-mRNA-1:cds;Parent=Msa1200850-mRNA-1
chr8_1 CDS 53821818 53821903 53821818 ID=Msa1200850-mRNA-1:cds;Parent=Msa1200850-mRNA-1
chr8_1 CDS 53821926 53822940 53821926 ID=Msa1200850-mRNA-1:cds;Parent=Msa1200850-mRNA-1
Gene Sequence

>Msa1200850

ATGAAAGGAATGAGTTATTTCCTCAACAACCATCCAGTGCTAAGATTCCTCTTCAAACTCAGTGTCGTGTCTCATGTCCATATCTGTATGTGCTTCATGGACAAGTTGGGATGGGATGGTTTTGTTGTTAATTTTGGAAGTTGTTTCAGTTTTGCCTTGAATCGAAACAACTTTTGGTCTGGATTGTCGGGAGAGGTTCTGCAGTTTTTAGAACCTCTTTTAGTCGTGTGGGGTGGTGGCTTGGTGCAAACTGATGGAAGAAAGGCAGCTAAAGTGCTCACGGTTTCATCCCCTTTTACAGTTTTGTATTGCACCCTTCAAGTTTCATTTCTGACAGCTCCCGATGCAGAAAAGTGCAGGAAGTATTACAGGCCACTTGCTTGGAGATGCATCTTTGCCGTTTTATCAGCCGGACCTTTGCCGAATCATATTGCTTTCATCATGGATGGGAATCGAAGGTACGCAAGGAGGAGAAACTTGGGAGAAGGGGATGGTCATAAGGCTGGATTTTCAGCTCTATTGTCTGTCCTTAGATACTGTTATGAATTGGGAATTAAGTATGTAACCGTTTATGCATTCAGCATTGATAACTTCAGAAGGAAGCCTAAAGAGGTTCAAACATTCATGGAATTGATGCGGGAAAAGATCGAGGAGTTGCTTCAACAGGAAAGCATTATTAATGAATACGGTGTTAGATTACATTTCATTGGAAACATGCAACTATTGACCGAACCTGTCAGGATTGCTGCAGAAAAGGCTATGAGAGTTACCGCCCACAACCAAGAGAGAGTTCTTTTAATTTGTGTGGCTTATACTTCTCGTGATGAGATTGTGCATGCTGTTCAAGAATCTTGCAAGGATAAATGGAACGAAATTCAACCATCCACGTCAGATAAAGTTTCGAATGGTGAAATTACAAGAATAAATCAGGGCCCGAAAAGAAACGGTCTTAATTTTCATTCTCACGATCCATGTAAAGAAAATGGAACCAAAGCTTGCAGTAATCATGAAGAAGGGGAAGGTGCTGGAGAGAAAGATGCTTTGTTTGAGCAAAACATTGAGAAAAGTAGCGATAATTGCAGTGAAGGTGAAATCACATCTTGTAACGGGAACATTGAAATAACTGAAGAGCAAAAGTACAACCAGGGTGAGATTGCTTCTATAAAACTAGTTGATATTGAGAAGCACATGTATATGACAGTGGCACCTGACCCAGATATCTTGATCAGAACGTCGGGAGAGGCACGTCTCAGTAATTTTCTTCTTTGGCAGACTAGTGCATGCCCTTTGTATGCACCAAAAGTACTTTGGCCTGAAATTGGTCTGAGACACTTGGTGTGGGCAGTATTGAACTTTCAGAGACACCATTTTTACTTGGAAAAGAAAAAGAAACAGTTTTGA
Protein sequence

>Msa1200850

MKGMSYFLNNHPVLRFLFKLSVVSHVHICMCFMDKLGWDGFVVNFGSCFSFALNRNNFWSGLSGEVLQFLEPLLVVWGGGLVQTDGRKAAKVLTVSSPFTVLYCTLQVSFLTAPDAEKCRKYYRPLAWRCIFAVLSAGPLPNHIAFIMDGNRRYARRRNLGEGDGHKAGFSALLSVLRYCYELGIKYVTVYAFSIDNFRRKPKEVQTFMELMREKIEELLQQESIINEYGVRLHFIGNMQLLTEPVRIAAEKAMRVTAHNQERVLLICVAYTSRDEIVHAVQESCKDKWNEIQPSTSDKVSNGEITRINQGPKRNGLNFHSHDPCKENGTKACSNHEEGEGAGEKDALFEQNIEKSSDNCSEGEITSCNGNIEITEEQKYNQGEIASIKLVDIEKHMYMTVAPDPDILIRTSGEARLSNFLLWQTSACPLYAPKVLWPEIGLRHLVWAVLNFQRHHFYLEKKKKQF*