AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1204750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204750 MtrunA17_Chr4g0053711 97.880 283 6 0 1 283 263 545 0.0 582
Msa1204750 MtrunA17_Chr8g0337661 43.750 288 126 10 2 276 274 538 1.20e-65 214
Msa1204750 MtrunA17_Chr8g0337651 44.523 283 133 9 2 276 274 540 1.31e-63 209
Msa1204750 MtrunA17_Chr1g0205391 39.011 182 110 1 3 184 226 406 5.31e-33 126
Msa1204750 MtrunA17_Chr3g0101121 34.091 176 107 2 1 176 191 357 3.92e-27 110
Msa1204750 MtrunA17_Chr8g0390621 33.333 192 117 3 28 217 231 413 1.86e-25 105
Msa1204750 MtrunA17_Chr5g0445281 31.183 186 116 3 1 186 216 389 4.50e-22 95.9
Msa1204750 MtrunA17_Chr2g0280141 36.601 153 92 2 31 181 669 818 1.57e-19 88.6
Msa1204750 MtrunA17_Chr5g0434761 34.839 155 92 4 31 182 116 264 8.79e-16 75.5
Msa1204750 MtrunA17_Chr2g0325711 34.375 160 95 4 9 162 663 818 4.34e-14 72.4
Msa1204750 MtrunA17_Chr6g0471251 28.846 156 109 2 25 179 612 766 1.44e-12 67.8
Msa1204750 MtrunA17_Chr2g0325611 30.769 130 89 1 37 166 447 575 8.44e-12 65.5
Msa1204750 MtrunA17_Chr8g0361651 29.710 138 93 3 43 177 268 404 1.89e-11 63.9
Msa1204750 MtrunA17_Chr2g0325661 32.331 133 82 3 43 173 739 865 1.98e-11 64.3
Msa1204750 MtrunA17_Chr2g0325351 31.008 129 88 1 37 165 545 672 3.79e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1204750 AT5G48385.1 50.754 199 93 4 2 199 279 473 4.26e-55 187
Msa1204750 AT3G22440.1 38.043 184 113 1 1 184 229 411 1.40e-32 125
Msa1204750 AT4G14900.1 38.587 184 112 1 1 184 227 409 2.07e-32 125
Msa1204750 AT5G16320.1 30.189 212 121 5 2 203 190 384 9.25e-23 97.8
Msa1204750 AT1G31814.1 28.095 210 131 4 1 199 182 382 2.08e-21 94.0
Msa1204750 AT5G27230.1 29.213 178 120 2 3 179 631 803 4.48e-19 87.4
Msa1204750 AT5G27220.4 22.464 276 191 5 1 270 868 1126 2.36e-13 70.5
Msa1204750 AT5G27220.2 22.464 276 191 5 1 270 868 1126 2.36e-13 70.5
Msa1204750 AT5G27220.1 22.263 274 190 5 3 270 925 1181 1.02e-12 68.9
Msa1204750 AT5G27220.3 22.263 274 190 5 3 270 925 1181 1.02e-12 68.9

Find 81 sgRNAs with CRISPR-Local

Find 224 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTGATACACTCCATAAA+AGG 0.188926 8_1:+60555774 Msa1204750:CDS
CCTGAGCTCTGCCGCTCTAT+TGG 0.259467 8_1:+60555126 Msa1204750:CDS
TGCAGCTTCTTTCTACTTAT+AGG 0.269952 8_1:+60555034 Msa1204750:CDS
TTAGGCCTTGGCCTCTTTGA+TGG 0.295724 8_1:-60555863 None:intergenic
GCTGCTTCAGCTGTCGTTAC+TGG 0.302021 8_1:+60555923 Msa1204750:CDS
ATGTGCAACTGCAGCATAAT+GGG 0.320159 8_1:-60555999 None:intergenic
TTACTTGTGAATTTCTCCTT+TGG 0.330915 8_1:-60555522 None:intergenic
TCTAACTATGGTCGTTATAT+AGG 0.346573 8_1:+60556076 Msa1204750:CDS
CCACTCATTGGCAATTGCCT+TGG 0.351735 8_1:-60554944 None:intergenic
TATGTCCTCAGGAGGGGAAC+AGG 0.354514 8_1:-60555490 None:intergenic
CCAATAGAGCGGCAGAGCTC+AGG 0.384993 8_1:-60555126 None:intergenic
ATTTGGTGACAAAACTCGTT+TGG 0.385535 8_1:+60556315 Msa1204750:three_prime_UTR
GTAAGAGTACACCTGCCTTC+TGG 0.398250 8_1:-60556248 None:intergenic
CATGTGCAACTGCAGCATAA+TGG 0.399358 8_1:-60556000 None:intergenic
GCACATGATTATCAAGTTCC+TGG 0.400769 8_1:+60556016 Msa1204750:CDS
CTTGGCCTCTTTGATGGTTG+AGG 0.414011 8_1:-60555857 None:intergenic
GTAAACTCAAACAAAACAAA+AGG 0.421886 8_1:-60556218 None:intergenic
AGTGCAACTCCTTCTAACTA+TGG 0.435897 8_1:+60556064 Msa1204750:CDS
TGAAGTGTATGTTAAACATT+TGG 0.438652 8_1:+60556298 Msa1204750:three_prime_UTR
TGCTGCCAATGGAAATTCAT+TGG 0.440898 8_1:+60554996 Msa1204750:CDS
CCACAGACTCGACCTACAAT+TGG 0.443526 8_1:-60556359 None:intergenic
AAATGCCTCTCGTTAGGCCT+TGG 0.463163 8_1:-60555875 None:intergenic
AAGGAGAAATTCACAAGTAA+AGG 0.464474 8_1:+60555525 Msa1204750:CDS
TTTGGCTAATACAATGGAGT+TGG 0.465440 8_1:+60556333 Msa1204750:three_prime_UTR
TAACTTGAGCCTCCACTCAT+TGG 0.468007 8_1:-60554956 None:intergenic
CCACTCCGTTCATCTGGTCG+TGG 0.468116 8_1:+60555899 Msa1204750:CDS
TCGAAGCTTTAATCAACAAT+GGG 0.470100 8_1:+60555410 Msa1204750:CDS
CATTTATATCCAATGGTCAC+TGG 0.473360 8_1:+60556270 Msa1204750:three_prime_UTR
AGCTGTCGTTACTGGTAGGC+AGG 0.474011 8_1:+60555931 Msa1204750:CDS
CAAGTCATTTCAGCAATGCT+TGG 0.479348 8_1:-60556143 None:intergenic
CCTGCATATGCTGTCCTAGC+AGG 0.482164 8_1:-60555959 None:intergenic
GGCTTAACTCACAAAGTGCC+AGG 0.482907 8_1:+60555147 Msa1204750:CDS
TTCATTTAACCAGTGACCAT+TGG 0.483090 8_1:-60556279 None:intergenic
ATCGAAGCTTTAATCAACAA+TGG 0.486111 8_1:+60555409 Msa1204750:CDS
ATATAACGACCATAGTTAGA+AGG 0.490939 8_1:-60556073 None:intergenic
TGCTTCCAATGAATTTCCAT+TGG 0.494489 8_1:-60555001 None:intergenic
AGTGGAAAATGCCTCTCGTT+AGG 0.497797 8_1:-60555881 None:intergenic
TGCAGCAAGCTCTTGTGCAT+TGG 0.499876 8_1:-60555699 None:intergenic
TGTCCTCAGGAGGGGAACAG+GGG 0.503282 8_1:-60555488 None:intergenic
TTAGAAGGAGTTGCACTAGC+TGG 0.503631 8_1:-60556058 None:intergenic
ATGTTAATCACATGTAATGA+TGG 0.506830 8_1:-60556120 None:intergenic
AACTCGTTTGGCTAATACAA+TGG 0.512219 8_1:+60556327 Msa1204750:three_prime_UTR
GTAGGCAGGTTCCGCCTGCT+AGG 0.514722 8_1:+60555945 Msa1204750:CDS
ACTTGATACACTCCATAAAA+GGG 0.528929 8_1:+60555775 Msa1204750:CDS
ATGTCCTCAGGAGGGGAACA+GGG 0.536265 8_1:-60555489 None:intergenic
TGGAGTTGGAAACCAATTGT+AGG 0.539364 8_1:+60556347 Msa1204750:three_prime_UTR
ACTCTTACATTTATATCCAA+TGG 0.540339 8_1:+60556263 Msa1204750:three_prime_UTR
TGGAAATTCATTGGAAGCAG+AGG 0.542065 8_1:+60555005 Msa1204750:CDS
CTGGCTGAGTGTACATAGAC+TGG 0.547487 8_1:-60556039 None:intergenic
TAAACTCAAACAAAACAAAA+GGG 0.551279 8_1:-60556217 None:intergenic
CCTGCTAGGACAGCATATGC+AGG 0.551952 8_1:+60555959 Msa1204750:CDS
GAGTGTATCAAGTGGGTACT+CGG 0.553451 8_1:-60555765 None:intergenic
CTTCAGCTGTCGTTACTGGT+AGG 0.554758 8_1:+60555927 Msa1204750:CDS
GACCTATTCTGAGCAACTGC+AGG 0.555204 8_1:-60555099 None:intergenic
AGGCTCAAGTTAGCTAGTGC+AGG 0.565107 8_1:+60554967 Msa1204750:CDS
CTCAACCATCAAAGAGGCCA+AGG 0.565712 8_1:+60555858 Msa1204750:CDS
TTACTATGATACCAGAAGGC+AGG 0.572042 8_1:+60556237 Msa1204750:three_prime_UTR
CATATGCAGGAACTGCTGAC+AGG 0.582210 8_1:+60555972 Msa1204750:CDS
TGTTAATCACATGTAATGAT+GGG 0.591110 8_1:-60556119 None:intergenic
TACCTGCAGTTGCTCAGAAT+AGG 0.593428 8_1:+60555097 Msa1204750:CDS
CCGTGATATTGCTACTGCAA+AGG 0.593436 8_1:+60555558 Msa1204750:CDS
CCAAGGCAATTGCCAATGAG+TGG 0.594398 8_1:+60554944 Msa1204750:CDS
TTGAGGTATGTCCTCAGGAG+GGG 0.596237 8_1:-60555496 None:intergenic
TGAGTGTACATAGACTGGCC+AGG 0.604971 8_1:-60556034 None:intergenic
GTCCTCAGGAGGGGAACAGG+GGG 0.606574 8_1:-60555487 None:intergenic
TTTGAGGTATGTCCTCAGGA+GGG 0.608468 8_1:-60555497 None:intergenic
GCAGGCATTGATGCTGCCAA+TGG 0.610237 8_1:+60554985 Msa1204750:CDS
GAGTTTACTATGATACCAGA+AGG 0.613997 8_1:+60556233 Msa1204750:three_prime_UTR
AACGTCCTCAACCATCAAAG+AGG 0.614792 8_1:+60555852 Msa1204750:CDS
TCACATGTAATGATGGGGTG+TGG 0.615343 8_1:-60556113 None:intergenic
CAGCACCACGACCAGATGAA+CGG 0.637196 8_1:-60555904 None:intergenic
AGGCAATTGCCAATGAGTGG+AGG 0.645365 8_1:+60554947 Msa1204750:CDS
TGCAGTTGCTCAGAATAGGT+CGG 0.649194 8_1:+60555101 Msa1204750:CDS
GATTGCTTCAGAGTTTGATG+AGG 0.655845 8_1:+60555056 Msa1204750:CDS
GCATATGCTGTCCTAGCAGG+CGG 0.659642 8_1:-60555956 None:intergenic
CCTTTGCAGTAGCAATATCA+CGG 0.661287 8_1:-60555558 None:intergenic
TGTGCAACTGCAGCATAATG+GGG 0.673444 8_1:-60555998 None:intergenic
AGAGGCCAAGGCCTAACGAG+AGG 0.675297 8_1:+60555870 Msa1204750:CDS
GTTAATCACATGTAATGATG+GGG 0.686353 8_1:-60556118 None:intergenic
CCACGACCAGATGAACGGAG+TGG 0.714251 8_1:-60555899 None:intergenic
TGTGAGTTAAGCCAATAGAG+CGG 0.723341 8_1:-60555137 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGGAATATTTAAAAAAAAGT+AGG - chr8_1:60556200-60556219 None:intergenic 20.0%
!! TAAACTCAAACAAAACAAAA+GGG - chr8_1:60556220-60556239 None:intergenic 20.0%
!! GGGAATATTTAAAAAAAAGT+AGG - chr8_1:60556200-60556219 None:intergenic 20.0%
!! TAAACTCAAACAAAACAAAA+GGG - chr8_1:60556220-60556239 None:intergenic 20.0%
! TGTTGTGGTATTATATTGTT+AGG + chr8_1:60555329-60555348 Msa1204750:intron 25.0%
! TCTCATTTAATTATTGTTGC+TGG + chr8_1:60555637-60555656 Msa1204750:intron 25.0%
! CTCATTTAATTATTGTTGCT+GGG + chr8_1:60555638-60555657 Msa1204750:intron 25.0%
! TGTTAATCACATGTAATGAT+GGG - chr8_1:60556122-60556141 None:intergenic 25.0%
! ATGTTAATCACATGTAATGA+TGG - chr8_1:60556123-60556142 None:intergenic 25.0%
! AAAAAGTAGGATAGAACTAA+TGG - chr8_1:60556187-60556206 None:intergenic 25.0%
! GTAAACTCAAACAAAACAAA+AGG - chr8_1:60556221-60556240 None:intergenic 25.0%
! ACTCTTACATTTATATCCAA+TGG + chr8_1:60556263-60556282 Msa1204750:three_prime_UTR 25.0%
! TGAAGTGTATGTTAAACATT+TGG + chr8_1:60556298-60556317 Msa1204750:three_prime_UTR 25.0%
! TGTTGTGGTATTATATTGTT+AGG + chr8_1:60555329-60555348 Msa1204750:intron 25.0%
! TCTCATTTAATTATTGTTGC+TGG + chr8_1:60555637-60555656 Msa1204750:intron 25.0%
! CTCATTTAATTATTGTTGCT+GGG + chr8_1:60555638-60555657 Msa1204750:intron 25.0%
! TGTTAATCACATGTAATGAT+GGG - chr8_1:60556122-60556141 None:intergenic 25.0%
! ATGTTAATCACATGTAATGA+TGG - chr8_1:60556123-60556142 None:intergenic 25.0%
! AAAAAGTAGGATAGAACTAA+TGG - chr8_1:60556187-60556206 None:intergenic 25.0%
! GTAAACTCAAACAAAACAAA+AGG - chr8_1:60556221-60556240 None:intergenic 25.0%
! ACTCTTACATTTATATCCAA+TGG + chr8_1:60556263-60556282 Msa1204750:three_prime_UTR 25.0%
! TGAAGTGTATGTTAAACATT+TGG + chr8_1:60556298-60556317 Msa1204750:three_prime_UTR 25.0%
TTATTAAAAGAACACCAACC+TGG - chr8_1:60555168-60555187 None:intergenic 30.0%
!! ATACTTTTCTGCTTTTGTTG+TGG + chr8_1:60555314-60555333 Msa1204750:intron 30.0%
TGTGGTATTATATTGTTAGG+TGG + chr8_1:60555332-60555351 Msa1204750:intron 30.0%
! ATCGAAGCTTTAATCAACAA+TGG + chr8_1:60555409-60555428 Msa1204750:CDS 30.0%
! TCGAAGCTTTAATCAACAAT+GGG + chr8_1:60555410-60555429 Msa1204750:CDS 30.0%
!!! AATTTCTCCTTTGGTTTTTG+AGG - chr8_1:60555516-60555535 None:intergenic 30.0%
TTACTTGTGAATTTCTCCTT+TGG - chr8_1:60555525-60555544 None:intergenic 30.0%
AAGGAGAAATTCACAAGTAA+AGG + chr8_1:60555525-60555544 Msa1204750:CDS 30.0%
! TTTTATGGAGTGTATCAAGT+GGG - chr8_1:60555775-60555794 None:intergenic 30.0%
ACTTGATACACTCCATAAAA+GGG + chr8_1:60555775-60555794 Msa1204750:CDS 30.0%
AGAAAGCAAAGACAGAAAAA+AGG + chr8_1:60555810-60555829 Msa1204750:CDS 30.0%
ATATAACGACCATAGTTAGA+AGG - chr8_1:60556076-60556095 None:intergenic 30.0%
TCTAACTATGGTCGTTATAT+AGG + chr8_1:60556076-60556095 Msa1204750:CDS 30.0%
GTTAATCACATGTAATGATG+GGG - chr8_1:60556121-60556140 None:intergenic 30.0%
TTATTAAAAGAACACCAACC+TGG - chr8_1:60555168-60555187 None:intergenic 30.0%
!! ATACTTTTCTGCTTTTGTTG+TGG + chr8_1:60555314-60555333 Msa1204750:intron 30.0%
TGTGGTATTATATTGTTAGG+TGG + chr8_1:60555332-60555351 Msa1204750:intron 30.0%
! ATCGAAGCTTTAATCAACAA+TGG + chr8_1:60555409-60555428 Msa1204750:CDS 30.0%
! TCGAAGCTTTAATCAACAAT+GGG + chr8_1:60555410-60555429 Msa1204750:CDS 30.0%
!!! AATTTCTCCTTTGGTTTTTG+AGG - chr8_1:60555516-60555535 None:intergenic 30.0%
TTACTTGTGAATTTCTCCTT+TGG - chr8_1:60555525-60555544 None:intergenic 30.0%
AAGGAGAAATTCACAAGTAA+AGG + chr8_1:60555525-60555544 Msa1204750:CDS 30.0%
! TTTTATGGAGTGTATCAAGT+GGG - chr8_1:60555775-60555794 None:intergenic 30.0%
ACTTGATACACTCCATAAAA+GGG + chr8_1:60555775-60555794 Msa1204750:CDS 30.0%
AGAAAGCAAAGACAGAAAAA+AGG + chr8_1:60555810-60555829 Msa1204750:CDS 30.0%
ATATAACGACCATAGTTAGA+AGG - chr8_1:60556076-60556095 None:intergenic 30.0%
TCTAACTATGGTCGTTATAT+AGG + chr8_1:60556076-60556095 Msa1204750:CDS 30.0%
GTTAATCACATGTAATGATG+GGG - chr8_1:60556121-60556140 None:intergenic 30.0%
TGCTTCCAATGAATTTCCAT+TGG - chr8_1:60555004-60555023 None:intergenic 35.0%
TGCAGCTTCTTTCTACTTAT+AGG + chr8_1:60555034-60555053 Msa1204750:CDS 35.0%
AAGACTGCTCATCTAGTATA+GGG - chr8_1:60555241-60555260 None:intergenic 35.0%
TAGATGAGCAGTCTTCTTTA+GGG + chr8_1:60555246-60555265 Msa1204750:intron 35.0%
! CTTTTATGGAGTGTATCAAG+TGG - chr8_1:60555776-60555795 None:intergenic 35.0%
CACTTGATACACTCCATAAA+AGG + chr8_1:60555774-60555793 Msa1204750:CDS 35.0%
!!! CTAGTTGATGAACCCTTTTA+TGG - chr8_1:60555790-60555809 None:intergenic 35.0%
GAGTTTACTATGATACCAGA+AGG + chr8_1:60556233-60556252 Msa1204750:three_prime_UTR 35.0%
CATTTATATCCAATGGTCAC+TGG + chr8_1:60556270-60556289 Msa1204750:three_prime_UTR 35.0%
TTCATTTAACCAGTGACCAT+TGG - chr8_1:60556282-60556301 None:intergenic 35.0%
! ATTTGGTGACAAAACTCGTT+TGG + chr8_1:60556315-60556334 Msa1204750:three_prime_UTR 35.0%
AACTCGTTTGGCTAATACAA+TGG + chr8_1:60556327-60556346 Msa1204750:three_prime_UTR 35.0%
TTTGGCTAATACAATGGAGT+TGG + chr8_1:60556333-60556352 Msa1204750:three_prime_UTR 35.0%
TGCTTCCAATGAATTTCCAT+TGG - chr8_1:60555004-60555023 None:intergenic 35.0%
TGCAGCTTCTTTCTACTTAT+AGG + chr8_1:60555034-60555053 Msa1204750:CDS 35.0%
AAGACTGCTCATCTAGTATA+GGG - chr8_1:60555241-60555260 None:intergenic 35.0%
TAGATGAGCAGTCTTCTTTA+GGG + chr8_1:60555246-60555265 Msa1204750:intron 35.0%
! CTTTTATGGAGTGTATCAAG+TGG - chr8_1:60555776-60555795 None:intergenic 35.0%
CACTTGATACACTCCATAAA+AGG + chr8_1:60555774-60555793 Msa1204750:CDS 35.0%
!!! CTAGTTGATGAACCCTTTTA+TGG - chr8_1:60555790-60555809 None:intergenic 35.0%
GAGTTTACTATGATACCAGA+AGG + chr8_1:60556233-60556252 Msa1204750:three_prime_UTR 35.0%
CATTTATATCCAATGGTCAC+TGG + chr8_1:60556270-60556289 Msa1204750:three_prime_UTR 35.0%
TTCATTTAACCAGTGACCAT+TGG - chr8_1:60556282-60556301 None:intergenic 35.0%
! ATTTGGTGACAAAACTCGTT+TGG + chr8_1:60556315-60556334 Msa1204750:three_prime_UTR 35.0%
AACTCGTTTGGCTAATACAA+TGG + chr8_1:60556327-60556346 Msa1204750:three_prime_UTR 35.0%
TTTGGCTAATACAATGGAGT+TGG + chr8_1:60556333-60556352 Msa1204750:three_prime_UTR 35.0%
TGCTGCCAATGGAAATTCAT+TGG + chr8_1:60554996-60555015 Msa1204750:CDS 40.0%
TGGAAATTCATTGGAAGCAG+AGG + chr8_1:60555005-60555024 Msa1204750:CDS 40.0%
GATTGCTTCAGAGTTTGATG+AGG + chr8_1:60555056-60555075 Msa1204750:CDS 40.0%
TGTGAGTTAAGCCAATAGAG+CGG - chr8_1:60555140-60555159 None:intergenic 40.0%
GTATAGGGGAGATATTTGAC+AGG - chr8_1:60555226-60555245 None:intergenic 40.0%
AGACTGCTCATCTAGTATAG+GGG - chr8_1:60555240-60555259 None:intergenic 40.0%
GAAGACTGCTCATCTAGTAT+AGG - chr8_1:60555242-60555261 None:intergenic 40.0%
CTAGATGAGCAGTCTTCTTT+AGG + chr8_1:60555245-60555264 Msa1204750:intron 40.0%
AGATGAGCAGTCTTCTTTAG+GGG + chr8_1:60555247-60555266 Msa1204750:intron 40.0%
! AGAAAAGTATATGTCACGCG+TGG - chr8_1:60555304-60555323 None:intergenic 40.0%
GGACATACCTCAAAAACCAA+AGG + chr8_1:60555506-60555525 Msa1204750:CDS 40.0%
CCTTTGCAGTAGCAATATCA+CGG - chr8_1:60555561-60555580 None:intergenic 40.0%
GCAAAGACAGAAAAAAGGAG+AGG + chr8_1:60555815-60555834 Msa1204750:CDS 40.0%
AAGACAGAAAAAAGGAGAGG+TGG + chr8_1:60555818-60555837 Msa1204750:CDS 40.0%
ATGTGCAACTGCAGCATAAT+GGG - chr8_1:60556002-60556021 None:intergenic 40.0%
GCACATGATTATCAAGTTCC+TGG + chr8_1:60556016-60556035 Msa1204750:CDS 40.0%
AGTGCAACTCCTTCTAACTA+TGG + chr8_1:60556064-60556083 Msa1204750:CDS 40.0%
CAAGTCATTTCAGCAATGCT+TGG - chr8_1:60556146-60556165 None:intergenic 40.0%
TTACTATGATACCAGAAGGC+AGG + chr8_1:60556237-60556256 Msa1204750:three_prime_UTR 40.0%
TGGAGTTGGAAACCAATTGT+AGG + chr8_1:60556347-60556366 Msa1204750:three_prime_UTR 40.0%
TGCTGCCAATGGAAATTCAT+TGG + chr8_1:60554996-60555015 Msa1204750:CDS 40.0%
TGGAAATTCATTGGAAGCAG+AGG + chr8_1:60555005-60555024 Msa1204750:CDS 40.0%
GATTGCTTCAGAGTTTGATG+AGG + chr8_1:60555056-60555075 Msa1204750:CDS 40.0%
TGTGAGTTAAGCCAATAGAG+CGG - chr8_1:60555140-60555159 None:intergenic 40.0%
GTATAGGGGAGATATTTGAC+AGG - chr8_1:60555226-60555245 None:intergenic 40.0%
AGACTGCTCATCTAGTATAG+GGG - chr8_1:60555240-60555259 None:intergenic 40.0%
GAAGACTGCTCATCTAGTAT+AGG - chr8_1:60555242-60555261 None:intergenic 40.0%
CTAGATGAGCAGTCTTCTTT+AGG + chr8_1:60555245-60555264 Msa1204750:intron 40.0%
AGATGAGCAGTCTTCTTTAG+GGG + chr8_1:60555247-60555266 Msa1204750:intron 40.0%
! AGAAAAGTATATGTCACGCG+TGG - chr8_1:60555304-60555323 None:intergenic 40.0%
GGACATACCTCAAAAACCAA+AGG + chr8_1:60555506-60555525 Msa1204750:CDS 40.0%
CCTTTGCAGTAGCAATATCA+CGG - chr8_1:60555561-60555580 None:intergenic 40.0%
GCAAAGACAGAAAAAAGGAG+AGG + chr8_1:60555815-60555834 Msa1204750:CDS 40.0%
AAGACAGAAAAAAGGAGAGG+TGG + chr8_1:60555818-60555837 Msa1204750:CDS 40.0%
ATGTGCAACTGCAGCATAAT+GGG - chr8_1:60556002-60556021 None:intergenic 40.0%
GCACATGATTATCAAGTTCC+TGG + chr8_1:60556016-60556035 Msa1204750:CDS 40.0%
AGTGCAACTCCTTCTAACTA+TGG + chr8_1:60556064-60556083 Msa1204750:CDS 40.0%
CAAGTCATTTCAGCAATGCT+TGG - chr8_1:60556146-60556165 None:intergenic 40.0%
TTACTATGATACCAGAAGGC+AGG + chr8_1:60556237-60556256 Msa1204750:three_prime_UTR 40.0%
TGGAGTTGGAAACCAATTGT+AGG + chr8_1:60556347-60556366 Msa1204750:three_prime_UTR 40.0%
TAACTTGAGCCTCCACTCAT+TGG - chr8_1:60554959-60554978 None:intergenic 45.0%
TACCTGCAGTTGCTCAGAAT+AGG + chr8_1:60555097-60555116 Msa1204750:CDS 45.0%
TGCAGTTGCTCAGAATAGGT+CGG + chr8_1:60555101-60555120 Msa1204750:CDS 45.0%
! TAACTCACAAAGTGCCAGGT+TGG + chr8_1:60555151-60555170 Msa1204750:intron 45.0%
! TTTGAGGTATGTCCTCAGGA+GGG - chr8_1:60555500-60555519 None:intergenic 45.0%
!!! TTTTGAGGTATGTCCTCAGG+AGG - chr8_1:60555501-60555520 None:intergenic 45.0%
!!! GGTTTTTGAGGTATGTCCTC+AGG - chr8_1:60555504-60555523 None:intergenic 45.0%
CCGTGATATTGCTACTGCAA+AGG + chr8_1:60555558-60555577 Msa1204750:CDS 45.0%
GAGTGTATCAAGTGGGTACT+CGG - chr8_1:60555768-60555787 None:intergenic 45.0%
AACGTCCTCAACCATCAAAG+AGG + chr8_1:60555852-60555871 Msa1204750:CDS 45.0%
AGTGGAAAATGCCTCTCGTT+AGG - chr8_1:60555884-60555903 None:intergenic 45.0%
! CATTTTCCACTCCGTTCATC+TGG + chr8_1:60555893-60555912 Msa1204750:CDS 45.0%
TGTGCAACTGCAGCATAATG+GGG - chr8_1:60556001-60556020 None:intergenic 45.0%
CATGTGCAACTGCAGCATAA+TGG - chr8_1:60556003-60556022 None:intergenic 45.0%
TTAGAAGGAGTTGCACTAGC+TGG - chr8_1:60556061-60556080 None:intergenic 45.0%
! TCACATGTAATGATGGGGTG+TGG - chr8_1:60556116-60556135 None:intergenic 45.0%
TAACTTGAGCCTCCACTCAT+TGG - chr8_1:60554959-60554978 None:intergenic 45.0%
TACCTGCAGTTGCTCAGAAT+AGG + chr8_1:60555097-60555116 Msa1204750:CDS 45.0%
TGCAGTTGCTCAGAATAGGT+CGG + chr8_1:60555101-60555120 Msa1204750:CDS 45.0%
! TAACTCACAAAGTGCCAGGT+TGG + chr8_1:60555151-60555170 Msa1204750:intron 45.0%
! TTTGAGGTATGTCCTCAGGA+GGG - chr8_1:60555500-60555519 None:intergenic 45.0%
!!! TTTTGAGGTATGTCCTCAGG+AGG - chr8_1:60555501-60555520 None:intergenic 45.0%
!!! GGTTTTTGAGGTATGTCCTC+AGG - chr8_1:60555504-60555523 None:intergenic 45.0%
CCGTGATATTGCTACTGCAA+AGG + chr8_1:60555558-60555577 Msa1204750:CDS 45.0%
GAGTGTATCAAGTGGGTACT+CGG - chr8_1:60555768-60555787 None:intergenic 45.0%
AACGTCCTCAACCATCAAAG+AGG + chr8_1:60555852-60555871 Msa1204750:CDS 45.0%
AGTGGAAAATGCCTCTCGTT+AGG - chr8_1:60555884-60555903 None:intergenic 45.0%
! CATTTTCCACTCCGTTCATC+TGG + chr8_1:60555893-60555912 Msa1204750:CDS 45.0%
TGTGCAACTGCAGCATAATG+GGG - chr8_1:60556001-60556020 None:intergenic 45.0%
CATGTGCAACTGCAGCATAA+TGG - chr8_1:60556003-60556022 None:intergenic 45.0%
TTAGAAGGAGTTGCACTAGC+TGG - chr8_1:60556061-60556080 None:intergenic 45.0%
! TCACATGTAATGATGGGGTG+TGG - chr8_1:60556116-60556135 None:intergenic 45.0%
CCACTCATTGGCAATTGCCT+TGG - chr8_1:60554947-60554966 None:intergenic 50.0%
CCAAGGCAATTGCCAATGAG+TGG + chr8_1:60554944-60554963 Msa1204750:CDS 50.0%
AGGCAATTGCCAATGAGTGG+AGG + chr8_1:60554947-60554966 Msa1204750:CDS 50.0%
AGGCTCAAGTTAGCTAGTGC+AGG + chr8_1:60554967-60554986 Msa1204750:CDS 50.0%
GACCTATTCTGAGCAACTGC+AGG - chr8_1:60555102-60555121 None:intergenic 50.0%
! GGCTTAACTCACAAAGTGCC+AGG + chr8_1:60555147-60555166 Msa1204750:CDS 50.0%
GACAGGACAATCCTTCACTG+CGG - chr8_1:60555209-60555228 None:intergenic 50.0%
TTGAGGTATGTCCTCAGGAG+GGG - chr8_1:60555499-60555518 None:intergenic 50.0%
TGCAGCAAGCTCTTGTGCAT+TGG - chr8_1:60555702-60555721 None:intergenic 50.0%
! CTTGGCCTCTTTGATGGTTG+AGG - chr8_1:60555860-60555879 None:intergenic 50.0%
CTCAACCATCAAAGAGGCCA+AGG + chr8_1:60555858-60555877 Msa1204750:CDS 50.0%
TTAGGCCTTGGCCTCTTTGA+TGG - chr8_1:60555866-60555885 None:intergenic 50.0%
AAATGCCTCTCGTTAGGCCT+TGG - chr8_1:60555878-60555897 None:intergenic 50.0%
CTTCAGCTGTCGTTACTGGT+AGG + chr8_1:60555927-60555946 Msa1204750:CDS 50.0%
CATATGCAGGAACTGCTGAC+AGG + chr8_1:60555972-60555991 Msa1204750:CDS 50.0%
! TGAGTGTACATAGACTGGCC+AGG - chr8_1:60556037-60556056 None:intergenic 50.0%
CTGGCTGAGTGTACATAGAC+TGG - chr8_1:60556042-60556061 None:intergenic 50.0%
! GTAAGAGTACACCTGCCTTC+TGG - chr8_1:60556251-60556270 None:intergenic 50.0%
CCACTCATTGGCAATTGCCT+TGG - chr8_1:60554947-60554966 None:intergenic 50.0%
CCAAGGCAATTGCCAATGAG+TGG + chr8_1:60554944-60554963 Msa1204750:CDS 50.0%
AGGCAATTGCCAATGAGTGG+AGG + chr8_1:60554947-60554966 Msa1204750:CDS 50.0%
AGGCTCAAGTTAGCTAGTGC+AGG + chr8_1:60554967-60554986 Msa1204750:CDS 50.0%
GACCTATTCTGAGCAACTGC+AGG - chr8_1:60555102-60555121 None:intergenic 50.0%
! GGCTTAACTCACAAAGTGCC+AGG + chr8_1:60555147-60555166 Msa1204750:CDS 50.0%
GACAGGACAATCCTTCACTG+CGG - chr8_1:60555209-60555228 None:intergenic 50.0%
TTGAGGTATGTCCTCAGGAG+GGG - chr8_1:60555499-60555518 None:intergenic 50.0%
TGCAGCAAGCTCTTGTGCAT+TGG - chr8_1:60555702-60555721 None:intergenic 50.0%
! CTTGGCCTCTTTGATGGTTG+AGG - chr8_1:60555860-60555879 None:intergenic 50.0%
CTCAACCATCAAAGAGGCCA+AGG + chr8_1:60555858-60555877 Msa1204750:CDS 50.0%
TTAGGCCTTGGCCTCTTTGA+TGG - chr8_1:60555866-60555885 None:intergenic 50.0%
AAATGCCTCTCGTTAGGCCT+TGG - chr8_1:60555878-60555897 None:intergenic 50.0%
CTTCAGCTGTCGTTACTGGT+AGG + chr8_1:60555927-60555946 Msa1204750:CDS 50.0%
CATATGCAGGAACTGCTGAC+AGG + chr8_1:60555972-60555991 Msa1204750:CDS 50.0%
! TGAGTGTACATAGACTGGCC+AGG - chr8_1:60556037-60556056 None:intergenic 50.0%
CTGGCTGAGTGTACATAGAC+TGG - chr8_1:60556042-60556061 None:intergenic 50.0%
! GTAAGAGTACACCTGCCTTC+TGG - chr8_1:60556251-60556270 None:intergenic 50.0%
!! GCAGGCATTGATGCTGCCAA+TGG + chr8_1:60554985-60555004 Msa1204750:CDS 55.0%
ACCTGTGAAGTCCGCAGTGA+AGG + chr8_1:60555195-60555214 Msa1204750:intron 55.0%
TCCTTCACTGCGGACTTCAC+AGG - chr8_1:60555199-60555218 None:intergenic 55.0%
ATGTCCTCAGGAGGGGAACA+GGG - chr8_1:60555492-60555511 None:intergenic 55.0%
TATGTCCTCAGGAGGGGAAC+AGG - chr8_1:60555493-60555512 None:intergenic 55.0%
CAGCACCACGACCAGATGAA+CGG - chr8_1:60555907-60555926 None:intergenic 55.0%
GCTGCTTCAGCTGTCGTTAC+TGG + chr8_1:60555923-60555942 Msa1204750:CDS 55.0%
AGCTGTCGTTACTGGTAGGC+AGG + chr8_1:60555931-60555950 Msa1204750:CDS 55.0%
GCATATGCTGTCCTAGCAGG+CGG - chr8_1:60555959-60555978 None:intergenic 55.0%
CCTGCATATGCTGTCCTAGC+AGG - chr8_1:60555962-60555981 None:intergenic 55.0%
CCTGCTAGGACAGCATATGC+AGG + chr8_1:60555959-60555978 Msa1204750:CDS 55.0%
!! GCAGGCATTGATGCTGCCAA+TGG + chr8_1:60554985-60555004 Msa1204750:CDS 55.0%
ACCTGTGAAGTCCGCAGTGA+AGG + chr8_1:60555195-60555214 Msa1204750:intron 55.0%
TCCTTCACTGCGGACTTCAC+AGG - chr8_1:60555199-60555218 None:intergenic 55.0%
ATGTCCTCAGGAGGGGAACA+GGG - chr8_1:60555492-60555511 None:intergenic 55.0%
TATGTCCTCAGGAGGGGAAC+AGG - chr8_1:60555493-60555512 None:intergenic 55.0%
CAGCACCACGACCAGATGAA+CGG - chr8_1:60555907-60555926 None:intergenic 55.0%
GCTGCTTCAGCTGTCGTTAC+TGG + chr8_1:60555923-60555942 Msa1204750:CDS 55.0%
AGCTGTCGTTACTGGTAGGC+AGG + chr8_1:60555931-60555950 Msa1204750:CDS 55.0%
GCATATGCTGTCCTAGCAGG+CGG - chr8_1:60555959-60555978 None:intergenic 55.0%
CCTGCATATGCTGTCCTAGC+AGG - chr8_1:60555962-60555981 None:intergenic 55.0%
CCTGCTAGGACAGCATATGC+AGG + chr8_1:60555959-60555978 Msa1204750:CDS 55.0%
CCAATAGAGCGGCAGAGCTC+AGG - chr8_1:60555129-60555148 None:intergenic 60.0%
CCTGAGCTCTGCCGCTCTAT+TGG + chr8_1:60555126-60555145 Msa1204750:CDS 60.0%
TGTCCTCAGGAGGGGAACAG+GGG - chr8_1:60555491-60555510 None:intergenic 60.0%
AGAGGCCAAGGCCTAACGAG+AGG + chr8_1:60555870-60555889 Msa1204750:CDS 60.0%
CCACGACCAGATGAACGGAG+TGG - chr8_1:60555902-60555921 None:intergenic 60.0%
CCACTCCGTTCATCTGGTCG+TGG + chr8_1:60555899-60555918 Msa1204750:CDS 60.0%
CCAATAGAGCGGCAGAGCTC+AGG - chr8_1:60555129-60555148 None:intergenic 60.0%
CCTGAGCTCTGCCGCTCTAT+TGG + chr8_1:60555126-60555145 Msa1204750:CDS 60.0%
TGTCCTCAGGAGGGGAACAG+GGG - chr8_1:60555491-60555510 None:intergenic 60.0%
AGAGGCCAAGGCCTAACGAG+AGG + chr8_1:60555870-60555889 Msa1204750:CDS 60.0%
CCACGACCAGATGAACGGAG+TGG - chr8_1:60555902-60555921 None:intergenic 60.0%
CCACTCCGTTCATCTGGTCG+TGG + chr8_1:60555899-60555918 Msa1204750:CDS 60.0%
TTCCCCCTGTTCCCCTCCTG+AGG + chr8_1:60555485-60555504 Msa1204750:CDS 65.0%
GTCCTCAGGAGGGGAACAGG+GGG - chr8_1:60555490-60555509 None:intergenic 65.0%
GTAGGCAGGTTCCGCCTGCT+AGG + chr8_1:60555945-60555964 Msa1204750:CDS 65.0%
TTCCCCCTGTTCCCCTCCTG+AGG + chr8_1:60555485-60555504 Msa1204750:CDS 65.0%
GTCCTCAGGAGGGGAACAGG+GGG - chr8_1:60555490-60555509 None:intergenic 65.0%
GTAGGCAGGTTCCGCCTGCT+AGG + chr8_1:60555945-60555964 Msa1204750:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 60554937 60556374 60554937 ID=Msa1204750;Name=Msa1204750
chr8_1 mRNA 60554937 60556374 60554937 ID=Msa1204750-mRNA-1;Parent=Msa1204750;Name=Msa1204750-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.5|0.33|1|1|1|3|239|283
chr8_1 exon 60554937 60555168 60554937 ID=Msa1204750-mRNA-1:exon:19641;Parent=Msa1204750-mRNA-1
chr8_1 exon 60555404 60555579 60555404 ID=Msa1204750-mRNA-1:exon:19642;Parent=Msa1204750-mRNA-1
chr8_1 exon 60555692 60556374 60555692 ID=Msa1204750-mRNA-1:exon:19643;Parent=Msa1204750-mRNA-1
chr8_1 CDS 60554937 60555168 60554937 ID=Msa1204750-mRNA-1:cds;Parent=Msa1204750-mRNA-1
chr8_1 CDS 60555404 60555579 60555404 ID=Msa1204750-mRNA-1:cds;Parent=Msa1204750-mRNA-1
chr8_1 CDS 60555692 60556135 60555692 ID=Msa1204750-mRNA-1:cds;Parent=Msa1204750-mRNA-1
chr8_1 three_prime_UTR 60556136 60556374 60556136 ID=Msa1204750-mRNA-1:three_prime_utr;Parent=Msa1204750-mRNA-1
Gene Sequence

>Msa1204750

CAGCAAGCCAAGGCAATTGCCAATGAGTGGAGGCTCAAGTTAGCTAGTGCAGGCATTGATGCTGCCAATGGAAATTCATTGGAAGCAGAGGCATTCTTGCAGCTTCTTTCTACTTATAGGATTGCTTCAGAGTTTGATGAGGAAGAACTCTGCAAGCTTGTACCTGCAGTTGCTCAGAATAGGTCGGCACCTGAGCTCTGCCGCTCTATTGGCTTAACTCACAAAGTGCCAGCTCTTATCGAAGCTTTAATCAACAATGGGAAACACATTGCAGCTGTACACTTTGTTCAACTCTTCAAGCTTCAAGAAAGCTTTCCCCCTGTTCCCCTCCTGAGGACATACCTCAAAAACCAAAGGAGAAATTCACAAGTAAAGGCTGATAATGTCCGTGATATTGCTACTGCAAAGATTGATGCCAATGCACAAGAGCTTGCTGCACTGAGAATTGTAATCAAGTGCATTGAAGAATATGAGTTCGAATCCGAGTACCCACTTGATACACTCCATAAAAGGGTTCATCAACTAGAGAAAGCAAAGACAGAAAAAAGGAGAGGTGGAGATTTTTTTAAACGTCCTCAACCATCAAAGAGGCCAAGGCCTAACGAGAGGCATTTTCCACTCCGTTCATCTGGTCGTGGTGCTGCTTCAGCTGTCGTTACTGGTAGGCAGGTTCCGCCTGCTAGGACAGCATATGCAGGAACTGCTGACAGGTATCCCCATTATGCTGCAGTTGCACATGATTATCAAGTTCCTGGCCAGTCTATGTACACTCAGCCAGCTAGTGCAACTCCTTCTAACTATGGTCGTTATATAGGTAGTAGTTTGCAATCCACACCCCATCATTACATGTGA
Protein sequence

>Msa1204750

QQAKAIANEWRLKLASAGIDAANGNSLEAEAFLQLLSTYRIASEFDEEELCKLVPAVAQNRSAPELCRSIGLTHKVPALIEALINNGKHIAAVHFVQLFKLQESFPPVPLLRTYLKNQRRNSQVKADNVRDIATAKIDANAQELAALRIVIKCIEEYEFESEYPLDTLHKRVHQLEKAKTEKRRGGDFFKRPQPSKRPRPNERHFPLRSSGRGAASAVVTGRQVPPARTAYAGTADRYPHYAAVAHDYQVPGQSMYTQPASATPSNYGRYIGSSLQSTPHHYM*