AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1208440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1208440 MtrunA17_Chr4g0057271 98.513 269 4 0 4 272 194 462 0.0 543
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1208440 AT1G12640.1 72.794 272 70 2 4 272 192 462 1.64e-134 388
Msa1208440 AT1G63050.1 69.853 272 78 2 4 272 195 465 5.39e-128 372

Find 62 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGATCTGGCAGGCCTTCC+AGG 0.216933 8_1:+66552806 None:intergenic
CACAGACCGTTAGTGCTATT+TGG 0.218362 8_1:-66553702 Msa1208440:CDS
CCTATTGTTATCATCCTTCT+TGG 0.234661 8_1:-66552842 Msa1208440:CDS
TTATTCCTGTGTTGGCTTCA+TGG 0.237906 8_1:-66553146 Msa1208440:intron
GATTTGTAGATTTGGAATTT+TGG 0.278800 8_1:-66554579 Msa1208440:intron
GCACCATATGGTGATGGTTT+TGG 0.315250 8_1:+66554546 None:intergenic
CATATCATGAATGGTGCTTC+TGG 0.319810 8_1:-66554431 Msa1208440:CDS
GCTCTGTCCCAACGCGGCTT+TGG 0.320400 8_1:+66554279 None:intergenic
TTCTATTATCATCTCTGGCT+TGG 0.327718 8_1:-66554331 Msa1208440:CDS
CTGGCTTGGGTTTCAGTGGC+TGG 0.334935 8_1:-66554317 Msa1208440:CDS
GTTGGAAATATTATTTCATT+TGG 0.336497 8_1:-66554368 Msa1208440:CDS
CAGTCAGCATTGATGATTGC+TGG 0.350154 8_1:-66553344 Msa1208440:CDS
CATGAAACTCTTGCCTCATA+TGG 0.355168 8_1:-66552893 Msa1208440:CDS
AACTTAGTCAGAGGAAAATA+AGG 0.357630 8_1:+66554465 None:intergenic
TCTATTATCATCTCTGGCTT+GGG 0.358985 8_1:-66554330 Msa1208440:CDS
GTTCTAAGTTATTCCTGTGT+TGG 0.360919 8_1:-66553154 Msa1208440:CDS
CTGTCCCAACGCGGCTTTGG+TGG 0.377191 8_1:+66554282 None:intergenic
GAAGCTTCTATTATCATCTC+TGG 0.389427 8_1:-66554336 Msa1208440:CDS
CTCTCCACCAAAGCCGCGTT+GGG 0.392120 8_1:-66554286 Msa1208440:CDS
CTGTTAAGCCAGACATATAC+TGG 0.392279 8_1:+66554394 None:intergenic
AAAATACACGCTTCCATATG+AGG 0.415312 8_1:+66552880 None:intergenic
CCAAGAAGGATGATAACAAT+AGG 0.436962 8_1:+66552842 None:intergenic
TTATTTCAGGGGTTGTATCC+TGG 0.439877 8_1:-66553386 Msa1208440:intron
TTGGGTTACCAGTATATGTC+TGG 0.449963 8_1:-66554402 Msa1208440:CDS
CCGAACCTTCATATCATGAA+TGG 0.466521 8_1:-66554440 Msa1208440:CDS
GCTCGAAGTGTTGCACCATA+TGG 0.482462 8_1:+66554534 None:intergenic
AAGTTTATAATCTATGACTA+AGG 0.483591 8_1:-66555567 None:intergenic
TGCCAGATCGAAAGCTCAAA+AGG 0.484458 8_1:-66552795 Msa1208440:CDS
ATCTCTGGCTTGGGTTTCAG+TGG 0.486889 8_1:-66554321 Msa1208440:CDS
GCAAGTAACTGAAGAAATCC+TGG 0.496211 8_1:+66553727 None:intergenic
CCATTCATGATATGAAGGTT+CGG 0.506149 8_1:+66554440 None:intergenic
GGACCAAAACCATCACCATA+TGG 0.507670 8_1:-66554549 Msa1208440:CDS
AAGCAAAATTAGCGAACACC+AGG 0.524642 8_1:+66553188 None:intergenic
CCTCTCCACCAAAGCCGCGT+TGG 0.529654 8_1:-66554287 Msa1208440:CDS
ACAAAGAATATAATGTATCC+AGG 0.530643 8_1:+66553368 None:intergenic
ATCCTGGCTTCTTCCCACTC+TGG 0.537883 8_1:+66553743 None:intergenic
AAGCACCATTCATGATATGA+AGG 0.537918 8_1:+66554435 None:intergenic
ATCCAGAGTGGGAAGAAGCC+AGG 0.538068 8_1:-66553745 Msa1208440:CDS
CTTGGTAAAGTGATCAAGCC+TGG 0.542234 8_1:-66552824 Msa1208440:CDS
ATGATAGGCTTATCCAGAGT+GGG 0.543235 8_1:-66553756 Msa1208440:CDS
ACTTGAATATTCCACACAGC+TGG 0.544819 8_1:+66554204 None:intergenic
GCTTGATCACTTTACCAAGA+AGG 0.546312 8_1:+66552828 None:intergenic
CTTCGAGCTCTACTTCAAGC+TGG 0.550167 8_1:-66554519 Msa1208440:CDS
TTCAAACAGTCATTTACAGA+TGG 0.556918 8_1:-66553243 Msa1208440:intron
AAACTCACAATGGCGAAGCC+AGG 0.558480 8_1:+66554176 None:intergenic
GTAAAGTGATCAAGCCTGGA+AGG 0.564430 8_1:-66552820 Msa1208440:CDS
GATGATAACAATAGGTAGAA+CGG 0.584053 8_1:+66552850 None:intergenic
CCAACGCGGCTTTGGTGGAG+AGG 0.585335 8_1:+66554287 None:intergenic
GGAATATTCAAGTCAGCACC+TGG 0.589501 8_1:-66554194 Msa1208440:CDS
CACATGCCAAATAGCACTAA+CGG 0.598601 8_1:+66553696 None:intergenic
TTGATGATTGCTGGTTCCAG+AGG 0.599868 8_1:-66553335 Msa1208440:intron
GTCATTTACAGATGGCAGCA+AGG 0.605840 8_1:-66553235 Msa1208440:CDS
AGTGTTGCACCATATGGTGA+TGG 0.624851 8_1:+66554540 None:intergenic
GGTTCGGTAAACTTAGTCAG+AGG 0.636544 8_1:+66554456 None:intergenic
TATGATAGGCTTATCCAGAG+TGG 0.639089 8_1:-66553757 Msa1208440:CDS
ACAAACCATGAAGCCAACAC+AGG 0.646385 8_1:+66553141 None:intergenic
AAAGCTCAAAAGGAAGAGTG+AGG 0.671201 8_1:-66552785 None:intergenic
AATAGCACTAACGGTCTGTG+TGG 0.678558 8_1:+66553705 None:intergenic
TCAGAGGAAAATAAGGCACG+AGG 0.693182 8_1:+66554472 None:intergenic
AGCAAAATTAGCGAACACCA+GGG 0.693283 8_1:+66553189 None:intergenic
TGTCTGGCTTAACAGCACGT+TGG 0.696548 8_1:-66554386 Msa1208440:CDS
CAGTTGTAATTCCAGCTGTG+TGG 0.697153 8_1:-66554215 Msa1208440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCAGCATAAATTAAATT+GGG + chr8_1:66553131-66553150 None:intergenic 15.0%
!! TTTATATCGGAAAAATAAAA+AGG - chr8_1:66553308-66553327 Msa1208440:intron 15.0%
!! TTATATCGGAAAAATAAAAA+GGG - chr8_1:66553309-66553328 Msa1208440:intron 15.0%
!!! TTTTTGTATAAACGATTTAT+TGG - chr8_1:66554341-66554360 Msa1208440:CDS 15.0%
!!! ATTTTGACATTTTTATTTCA+GGG - chr8_1:66554943-66554962 Msa1208440:intron 15.0%
!! CTTATCAGCATAAATTAAAT+TGG + chr8_1:66553132-66553151 None:intergenic 20.0%
!!! AAGGATAGTCTTTTTTATAT+CGG - chr8_1:66553295-66553314 Msa1208440:intron 20.0%
!! GTTGGAAATATTATTTCATT+TGG - chr8_1:66553973-66553992 Msa1208440:intron 20.0%
!!! GAAGAAAGATTATTTTAACA+TGG + chr8_1:66554368-66554387 None:intergenic 20.0%
!!! TTTTTGTAGATGTTTATGAT+AGG - chr8_1:66554570-66554589 Msa1208440:CDS 20.0%
!!! CTTTCTATTTCTACTATATT+TGG - chr8_1:66554712-66554731 Msa1208440:intron 20.0%
!!! ATATGAATTTTTCTTGTCAA+CGG + chr8_1:66554919-66554938 None:intergenic 20.0%
!!! CATTTTGACATTTTTATTTC+AGG - chr8_1:66554942-66554961 Msa1208440:intron 20.0%
!!! TTTTGACATTTTTATTTCAG+GGG - chr8_1:66554944-66554963 Msa1208440:intron 20.0%
! TATGCTTATACTGTCATTAT+AGG + chr8_1:66552853-66552872 None:intergenic 25.0%
!!! GAAAAATAAAAAGGGCATTT+TGG - chr8_1:66553317-66553336 Msa1208440:intron 25.0%
! TTGATTTAGAAACTGATCAT+GGG + chr8_1:66553441-66553460 None:intergenic 25.0%
!! TTTGATTTAGAAACTGATCA+TGG + chr8_1:66553442-66553461 None:intergenic 25.0%
! ATCAGTTTCTAAATCAAACA+TGG - chr8_1:66553444-66553463 Msa1208440:intron 25.0%
! TTTATTAGTGTTGTCAAATG+TGG - chr8_1:66553540-66553559 Msa1208440:intron 25.0%
! TTGTTAAATAGCAGTTATAG+AGG + chr8_1:66553720-66553739 None:intergenic 25.0%
!!! GATTTGTAGATTTGGAATTT+TGG - chr8_1:66553762-66553781 Msa1208440:CDS 25.0%
! GCTAAAAATGTGGATATATT+AGG - chr8_1:66554083-66554102 Msa1208440:intron 25.0%
! TTAAGTTTCATGCGTAAAAT+TGG + chr8_1:66554232-66554251 None:intergenic 25.0%
! TTTACGCATGAAACTTAAAT+AGG - chr8_1:66554234-66554253 Msa1208440:CDS 25.0%
! TTACGCATGAAACTTAAATA+GGG - chr8_1:66554235-66554254 Msa1208440:CDS 25.0%
! AAATAACTAAAGAACCAAAC+AGG + chr8_1:66554324-66554343 None:intergenic 25.0%
! ACAAAGAATATAATGTATCC+AGG + chr8_1:66554976-66554995 None:intergenic 25.0%
! TAATTCAATATCTATACCTC+TGG + chr8_1:66555025-66555044 None:intergenic 25.0%
!! AGAGGTATAGATATTGAATT+AGG - chr8_1:66555024-66555043 Msa1208440:intron 25.0%
! GTAAATCTGATCATACAAAT+TGG + chr8_1:66555356-66555375 None:intergenic 25.0%
AGTAGTGATAATAATGACAG+TGG - chr8_1:66552944-66552963 Msa1208440:intron 30.0%
AATTAAATTGGGCATGCATT+CGG + chr8_1:66553120-66553139 None:intergenic 30.0%
! ATGCCCAATTTATCATGTTT+TGG - chr8_1:66553185-66553204 Msa1208440:CDS 30.0%
! TGCCCAATTTATCATGTTTT+GGG - chr8_1:66553186-66553205 Msa1208440:CDS 30.0%
ACCCCAAAACATGATAAATT+GGG + chr8_1:66553191-66553210 None:intergenic 30.0%
ATTCGTAAAACGTTAAGTAC+CGG - chr8_1:66553218-66553237 Msa1208440:CDS 30.0%
GTGTTATGTTCTATCTTATC+CGG + chr8_1:66553240-66553259 None:intergenic 30.0%
!! TGGGACAAAAAAGGTATTAA+GGG + chr8_1:66553422-66553441 None:intergenic 30.0%
!! ATGGGACAAAAAAGGTATTA+AGG + chr8_1:66553423-66553442 None:intergenic 30.0%
GTACAAAATGTTGTCAAGTA+AGG + chr8_1:66553630-66553649 None:intergenic 30.0%
ATAGAGGCAAAATATCGTTA+TGG + chr8_1:66553704-66553723 None:intergenic 30.0%
! GTTGTTGTGATTTGTAGATT+TGG - chr8_1:66553754-66553773 Msa1208440:CDS 30.0%
!! ATTTGGAATTTTGGTGCAAA+AGG - chr8_1:66553771-66553790 Msa1208440:intron 30.0%
AACTTAGTCAGAGGAAAATA+AGG + chr8_1:66553879-66553898 None:intergenic 30.0%
TAAAGAACTGAAACTCACAA+TGG + chr8_1:66554178-66554197 None:intergenic 30.0%
! TTCAAGTTCTGGTAATAGTA+TGG - chr8_1:66554274-66554293 Msa1208440:CDS 30.0%
!!! GCTAGATTAGTTTTTCATGT+TGG - chr8_1:66554296-66554315 Msa1208440:CDS 30.0%
! TTCAGCTATAACATCTGTTT+TGG - chr8_1:66554388-66554407 Msa1208440:CDS 30.0%
AAACTATGAAAATCAACTGC+TGG + chr8_1:66554443-66554462 None:intergenic 30.0%
GATGCAAAATTGAAATGTGA+AGG - chr8_1:66554504-66554523 Msa1208440:CDS 30.0%
GAATGCATTTGTAGTTTCTT+CGG - chr8_1:66554777-66554796 Msa1208440:intron 30.0%
TTCAAACAGTCATTTACAGA+TGG - chr8_1:66555098-66555117 Msa1208440:intron 30.0%
! TAATTTTGCTTACACACTTC+TGG - chr8_1:66555165-66555184 Msa1208440:intron 30.0%
!!! TATGGAAGCGTGTATTTTTT+TGG - chr8_1:66555466-66555485 Msa1208440:intron 30.0%
GATGATAACAATAGGTAGAA+CGG + chr8_1:66555494-66555513 None:intergenic 30.0%
! GCCCAATTTATCATGTTTTG+GGG - chr8_1:66553187-66553206 Msa1208440:CDS 35.0%
CACCCCAAAACATGATAAAT+TGG + chr8_1:66553192-66553211 None:intergenic 35.0%
TAGACAAGACAAAACTGTAC+TGG - chr8_1:66553262-66553281 Msa1208440:intron 35.0%
AAAACTGTACTGGAGAAAGA+TGG - chr8_1:66553272-66553291 Msa1208440:intron 35.0%
AACTGATCATGGGACAAAAA+AGG + chr8_1:66553431-66553450 None:intergenic 35.0%
TAAAAACCAAAGAACTCGAC+TGG + chr8_1:66553485-66553504 None:intergenic 35.0%
AATGTGGATAGCAGGAAATA+TGG - chr8_1:66553556-66553575 Msa1208440:intron 35.0%
ATGTGGATAGCAGGAAATAT+GGG - chr8_1:66553557-66553576 Msa1208440:intron 35.0%
TTGTCAAGTAAGGACTATAG+TGG + chr8_1:66553620-66553639 None:intergenic 35.0%
!!! ACTTCAAGCTGGTTTTTGTA+TGG - chr8_1:66553833-66553852 Msa1208440:intron 35.0%
!!! CTTCAAGCTGGTTTTTGTAT+GGG - chr8_1:66553834-66553853 Msa1208440:intron 35.0%
CCATTCATGATATGAAGGTT+CGG + chr8_1:66553904-66553923 None:intergenic 35.0%
AAGCACCATTCATGATATGA+AGG + chr8_1:66553909-66553928 None:intergenic 35.0%
GAAGCTTCTATTATCATCTC+TGG - chr8_1:66554005-66554024 Msa1208440:intron 35.0%
TTCTATTATCATCTCTGGCT+TGG - chr8_1:66554010-66554029 Msa1208440:intron 35.0%
! TCTATTATCATCTCTGGCTT+GGG - chr8_1:66554011-66554030 Msa1208440:intron 35.0%
GTTTCAGTTCTTTACTTGCA+CGG - chr8_1:66554184-66554203 Msa1208440:CDS 35.0%
!!! ACTTGATGGTTTTCAAGTTC+TGG - chr8_1:66554263-66554282 Msa1208440:CDS 35.0%
! TTTTCATGTTGGTACCTGTT+TGG - chr8_1:66554307-66554326 Msa1208440:CDS 35.0%
TTGTTCCTACCCTTTAAACT+AGG - chr8_1:66554478-66554497 Msa1208440:CDS 35.0%
TGCATCTAGCCTAGTTTAAA+GGG + chr8_1:66554490-66554509 None:intergenic 35.0%
TTGCATCTAGCCTAGTTTAA+AGG + chr8_1:66554491-66554510 None:intergenic 35.0%
TTCTTCGGAAAAACCAACAA+AGG - chr8_1:66554792-66554811 Msa1208440:intron 35.0%
ATGACTATCTGTTCCTTTGT+TGG + chr8_1:66554808-66554827 None:intergenic 35.0%
ATCACTCTTGTGAATTGTGA+TGG - chr8_1:66554835-66554854 Msa1208440:intron 35.0%
! GTTCTAAGTTATTCCTGTGT+TGG - chr8_1:66555187-66555206 Msa1208440:intron 35.0%
TTTGTGTGCTGTGTTTGAAT+AGG - chr8_1:66555414-66555433 Msa1208440:intron 35.0%
AAAATACACGCTTCCATATG+AGG + chr8_1:66555464-66555483 None:intergenic 35.0%
CCAAGAAGGATGATAACAAT+AGG + chr8_1:66555502-66555521 None:intergenic 35.0%
CCTATTGTTATCATCCTTCT+TGG - chr8_1:66555499-66555518 Msa1208440:intron 35.0%
AGATGTCCCATGGTTTAGTA+AGG - chr8_1:66553370-66553389 Msa1208440:CDS 40.0%
GATGTCCCATGGTTTAGTAA+GGG - chr8_1:66553371-66553390 Msa1208440:CDS 40.0%
TGTTGTCAAATGTGGATAGC+AGG - chr8_1:66553548-66553567 Msa1208440:intron 40.0%
TGTTTAGATTCCTCTATGCC+AGG - chr8_1:66553678-66553697 Msa1208440:intron 40.0%
CCGAACCTTCATATCATGAA+TGG - chr8_1:66553901-66553920 Msa1208440:intron 40.0%
CATATCATGAATGGTGCTTC+TGG - chr8_1:66553910-66553929 Msa1208440:intron 40.0%
! ATGGTGCTTCTGGAAAAAGT+TGG - chr8_1:66553920-66553939 Msa1208440:intron 40.0%
! TGGTGCTTCTGGAAAAAGTT+GGG - chr8_1:66553921-66553940 Msa1208440:intron 40.0%
TTGGGTTACCAGTATATGTC+TGG - chr8_1:66553939-66553958 Msa1208440:intron 40.0%
CTGTTAAGCCAGACATATAC+TGG + chr8_1:66553950-66553969 None:intergenic 40.0%
TTGGGACAGAGCTAAAAATG+TGG - chr8_1:66554073-66554092 Msa1208440:intron 40.0%
ACTTGAATATTCCACACAGC+TGG + chr8_1:66554140-66554159 None:intergenic 40.0%
TAAATAGGGTGCAGACTTGA+TGG - chr8_1:66554249-66554268 Msa1208440:CDS 40.0%
CTATGAAAATCAACTGCTGG+TGG + chr8_1:66554440-66554459 None:intergenic 40.0%
TCTAGCCTAGTTTAAAGGGT+AGG + chr8_1:66554486-66554505 None:intergenic 40.0%
TATGATAGGCTTATCCAGAG+TGG - chr8_1:66554584-66554603 Msa1208440:intron 40.0%
ATGATAGGCTTATCCAGAGT+GGG - chr8_1:66554585-66554604 Msa1208440:intron 40.0%
GCAAGTAACTGAAGAAATCC+TGG + chr8_1:66554617-66554636 None:intergenic 40.0%
CACATGCCAAATAGCACTAA+CGG + chr8_1:66554648-66554667 None:intergenic 40.0%
TTATTTCAGGGGTTGTATCC+TGG - chr8_1:66554955-66554974 Msa1208440:intron 40.0%
!! CAGTTAATTAACTTGCGCTG+CGG - chr8_1:66555067-66555086 Msa1208440:intron 40.0%
!! AGGGCATTTTTAACTGTTGG+AGG + chr8_1:66555136-66555155 None:intergenic 40.0%
TCCAACAGTTAAAAATGCCC+TGG - chr8_1:66555135-66555154 Msa1208440:intron 40.0%
!! ACCAGGGCATTTTTAACTGT+TGG + chr8_1:66555139-66555158 None:intergenic 40.0%
AGCAAAATTAGCGAACACCA+GGG + chr8_1:66555155-66555174 None:intergenic 40.0%
AAGCAAAATTAGCGAACACC+AGG + chr8_1:66555156-66555175 None:intergenic 40.0%
!! TTATTCCTGTGTTGGCTTCA+TGG - chr8_1:66555195-66555214 Msa1208440:intron 40.0%
CATGAAACTCTTGCCTCATA+TGG - chr8_1:66555448-66555467 Msa1208440:intron 40.0%
GCTTGATCACTTTACCAAGA+AGG + chr8_1:66555516-66555535 None:intergenic 40.0%
! TTCCTTTTGAGCTTTCGATC+TGG + chr8_1:66555551-66555570 None:intergenic 40.0%
CTGTACTGGAGAAAGATGGA+AGG - chr8_1:66553276-66553295 Msa1208440:intron 45.0%
CTAGATCGAAAGATGTCCCA+TGG - chr8_1:66553360-66553379 Msa1208440:CDS 45.0%
CGTGTCCCTTACTAAACCAT+GGG + chr8_1:66553379-66553398 None:intergenic 45.0%
TCGTGTCCCTTACTAAACCA+TGG + chr8_1:66553380-66553399 None:intergenic 45.0%
AGTTGTCCAGTCGAGTTCTT+TGG - chr8_1:66553476-66553495 Msa1208440:intron 45.0%
GCTATGCAACAGTGCTATAG+CGG - chr8_1:66553594-66553613 Msa1208440:intron 45.0%
GGCAAAATATCGTTATGGCC+TGG + chr8_1:66553699-66553718 None:intergenic 45.0%
GGACCAAAACCATCACCATA+TGG - chr8_1:66553792-66553811 Msa1208440:intron 45.0%
!!! GCACCATATGGTGATGGTTT+TGG + chr8_1:66553798-66553817 None:intergenic 45.0%
AGTGTTGCACCATATGGTGA+TGG + chr8_1:66553804-66553823 None:intergenic 45.0%
TCAGAGGAAAATAAGGCACG+AGG + chr8_1:66553872-66553891 None:intergenic 45.0%
! GGTTCGGTAAACTTAGTCAG+AGG + chr8_1:66553888-66553907 None:intergenic 45.0%
!!! TTTTTAGCTCTGTCCCAACG+CGG + chr8_1:66554071-66554090 None:intergenic 45.0%
CAGTTGTAATTCCAGCTGTG+TGG - chr8_1:66554126-66554145 Msa1208440:intron 45.0%
GGAATATTCAAGTCAGCACC+TGG - chr8_1:66554147-66554166 Msa1208440:intron 45.0%
AATAGCACTAACGGTCTGTG+TGG + chr8_1:66554639-66554658 None:intergenic 45.0%
! CACAGACCGTTAGTGCTATT+TGG - chr8_1:66554639-66554658 Msa1208440:intron 45.0%
TACCACGAAACAACTTTGCC+AGG - chr8_1:66554880-66554899 Msa1208440:intron 45.0%
AACGGTACAAAATAGCAGCC+TGG + chr8_1:66554901-66554920 None:intergenic 45.0%
!! CAGTCAGCATTGATGATTGC+TGG - chr8_1:66554997-66555016 Msa1208440:intron 45.0%
!! TTGATGATTGCTGGTTCCAG+AGG - chr8_1:66555006-66555025 Msa1208440:intron 45.0%
GTCATTTACAGATGGCAGCA+AGG - chr8_1:66555106-66555125 Msa1208440:intron 45.0%
ACAAACCATGAAGCCAACAC+AGG + chr8_1:66555203-66555222 None:intergenic 45.0%
CTTGGTAAAGTGATCAAGCC+TGG - chr8_1:66555517-66555536 Msa1208440:intron 45.0%
GTAAAGTGATCAAGCCTGGA+AGG - chr8_1:66555521-66555540 Msa1208440:intron 45.0%
! TTTTGAGCTTTCGATCTGGC+AGG + chr8_1:66555547-66555566 None:intergenic 45.0%
TGCCAGATCGAAAGCTCAAA+AGG - chr8_1:66555546-66555565 Msa1208440:intron 45.0%
ATCGTTATGGCCTGGCATAG+AGG + chr8_1:66553691-66553710 None:intergenic 50.0%
!! GCTCGAAGTGTTGCACCATA+TGG + chr8_1:66553810-66553829 None:intergenic 50.0%
CTTCGAGCTCTACTTCAAGC+TGG - chr8_1:66553822-66553841 Msa1208440:intron 50.0%
! TGTCTGGCTTAACAGCACGT+TGG - chr8_1:66553955-66553974 Msa1208440:intron 50.0%
!! ATCTCTGGCTTGGGTTTCAG+TGG - chr8_1:66554020-66554039 Msa1208440:intron 50.0%
AAACTCACAATGGCGAAGCC+AGG + chr8_1:66554168-66554187 None:intergenic 50.0%
! AGCCTGGCAAAGTTGTTTCG+TGG + chr8_1:66554885-66554904 None:intergenic 50.0%
ATCCAGAGTGGGAAGAAGCC+AGG - chr8_1:66554596-66554615 Msa1208440:intron 55.0%
ATCCTGGCTTCTTCCCACTC+TGG + chr8_1:66554601-66554620 None:intergenic 55.0%
!! CTGGCTTGGGTTTCAGTGGC+TGG - chr8_1:66554024-66554043 Msa1208440:intron 60.0%
CTCTCCACCAAAGCCGCGTT+GGG - chr8_1:66554055-66554074 Msa1208440:intron 60.0%
TTCGATCTGGCAGGCCTTCC+AGG + chr8_1:66555538-66555557 None:intergenic 60.0%
! CCAACGCGGCTTTGGTGGAG+AGG + chr8_1:66554057-66554076 None:intergenic 65.0%
CCTCTCCACCAAAGCCGCGT+TGG - chr8_1:66554054-66554073 Msa1208440:intron 65.0%
! CTGTCCCAACGCGGCTTTGG+TGG + chr8_1:66554062-66554081 None:intergenic 65.0%
GCTCTGTCCCAACGCGGCTT+TGG + chr8_1:66554065-66554084 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 66552787 66555576 66552787 ID=Msa1208440;Name=Msa1208440
chr8_1 mRNA 66552787 66555576 66552787 ID=Msa1208440-mRNA-1;Parent=Msa1208440;Name=Msa1208440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|0.83|1|1|6|0|272
chr8_1 exon 66555568 66555576 66555568 ID=Msa1208440-mRNA-1:exon:21916;Parent=Msa1208440-mRNA-1
chr8_1 exon 66554184 66554592 66554184 ID=Msa1208440-mRNA-1:exon:21915;Parent=Msa1208440-mRNA-1
chr8_1 exon 66553699 66553784 66553699 ID=Msa1208440-mRNA-1:exon:21914;Parent=Msa1208440-mRNA-1
chr8_1 exon 66553336 66553399 66553336 ID=Msa1208440-mRNA-1:exon:21913;Parent=Msa1208440-mRNA-1
chr8_1 exon 66553147 66553256 66553147 ID=Msa1208440-mRNA-1:exon:21912;Parent=Msa1208440-mRNA-1
chr8_1 exon 66552787 66552927 66552787 ID=Msa1208440-mRNA-1:exon:21911;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66555568 66555576 66555568 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66554184 66554592 66554184 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66553699 66553784 66553699 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66553336 66553399 66553336 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66553147 66553256 66553147 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
chr8_1 CDS 66552787 66552927 66552787 ID=Msa1208440-mRNA-1:cds;Parent=Msa1208440-mRNA-1
Gene Sequence

>Msa1208440

ATGACTAAGATTTGGAATTTTGGTGCAAAAGGACCAAAACCATCACCATATGGTGCAACACTTCGAGCTCTACTTCAAGCTGGTTTTTGTATGGGATTGTACTTAAACCTCGTGCCTTATTTTCCTCTGACTAAGTTTACCGAACCTTCATATCATGAATGGTGCTTCTGGAAAAAGTTGGGTTACCAGTATATGTCTGGCTTAACAGCACGTTGGAAATATTATTTCATTTGGTCGATTTCAGAAGCTTCTATTATCATCTCTGGCTTGGGTTTCAGTGGCTGGACTGATTCCTCTCCACCAAAGCCGCGTTGGGACAGAGCTAAAAATGTGGATATATTAGGTGTTGAGTTTGCAAAGAGTGCAGTTGTAATTCCAGCTGTGTGGAATATTCAAGTCAGCACCTGGCTTCGCCATTATGTTTATGATAGGCTTATCCAGAGTGGGAAGAAGCCAGGATTTCTTCAGTTACTTGCCACACAGACCGTTAGTGCTATTTGGCATGGGTTGTATCCTGGATACATTATATTCTTTGTTCAGTCAGCATTGATGATTGCTGGTTCCAGAGTCATTTACAGATGGCAGCAAGGCGTGCCTCCAACAGTTAAAAATGCCCTGGTGTTCGCTAATTTTGCTTACACACTTCTGGTTCTAAGTTATTCCTGTGTTGGCTTCATGGTATTAAGTCTGCATGAAACTCTTGCCTCATATGGAAGCGTGTATTTTTTTGGAACCGTTCTACCTATTGTTATCATCCTTCTTGGTAAAGTGATCAAGCCTGGAAGGCCTGCCAGATCGAAAGCTCAAAAGGAAGAGTGA
Protein sequence

>Msa1208440

MTKIWNFGAKGPKPSPYGATLRALLQAGFCMGLYLNLVPYFPLTKFTEPSYHEWCFWKKLGYQYMSGLTARWKYYFIWSISEASIIISGLGFSGWTDSSPPKPRWDRAKNVDILGVEFAKSAVVIPAVWNIQVSTWLRHYVYDRLIQSGKKPGFLQLLATQTVSAIWHGLYPGYIIFFVQSALMIAGSRVIYRWQQGVPPTVKNALVFANFAYTLLVLSYSCVGFMVLSLHETLASYGSVYFFGTVLPIVIILLGKVIKPGRPARSKAQKEE*