AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1211720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211720 MtrunA17_Chr1g0183651 60.920 87 26 1 10 88 384 470 7.67e-29 108
Msa1211720 MtrunA17_Chr1g0187581 52.326 86 33 1 11 88 383 468 2.39e-23 93.2
Msa1211720 MtrunA17_Chr1g0167351 71.186 59 17 0 29 87 403 461 2.52e-23 93.2
Msa1211720 MtrunA17_Chr1g0167311 47.059 85 37 1 11 87 381 465 2.69e-19 81.6
Msa1211720 MtrunA17_Chr7g0261491 49.367 79 39 1 11 88 397 475 1.35e-18 79.7
Msa1211720 MtrunA17_Chr3g0135951 52.542 59 28 0 29 87 394 452 5.58e-14 66.6
Msa1211720 MtrunA17_Chr2g0299781 49.153 59 30 0 29 87 413 471 5.32e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1211720 AT1G03920.2 62.069 58 22 0 31 88 317 374 3.08e-20 84.7
Msa1211720 AT1G03920.1 62.069 58 22 0 31 88 430 487 3.39e-20 84.7
Msa1211720 AT1G03920.3 62.069 58 22 0 31 88 430 487 3.39e-20 84.7
Msa1211720 AT2G20470.3 45.349 86 39 1 11 88 388 473 6.26e-20 84.0
Msa1211720 AT2G20470.2 45.349 86 39 1 11 88 388 473 8.30e-20 83.6
Msa1211720 AT2G20470.1 45.349 86 39 1 11 88 388 473 8.30e-20 83.6
Msa1211720 AT3G23310.1 43.023 86 41 1 11 88 388 473 2.61e-17 76.6
Msa1211720 AT4G14350.2 55.000 60 27 0 29 88 411 470 5.29e-17 75.5
Msa1211720 AT4G14350.1 55.000 60 27 0 29 88 411 470 5.29e-17 75.5
Msa1211720 AT4G14350.4 55.000 60 27 0 29 88 411 470 5.29e-17 75.5
Msa1211720 AT4G14350.3 55.000 60 27 0 29 88 411 470 5.66e-17 75.5
Msa1211720 AT4G33080.1 52.727 55 26 0 33 87 399 453 1.51e-13 65.9
Msa1211720 AT4G33080.2 52.727 55 26 0 33 87 399 453 1.52e-13 65.9
Msa1211720 AT1G30640.3 37.209 86 46 1 11 88 297 382 2.54e-13 65.1
Msa1211720 AT1G30640.2 37.209 86 46 1 11 88 390 475 2.90e-13 65.1
Msa1211720 AT1G30640.1 37.209 86 46 1 11 88 390 475 2.90e-13 65.1
Msa1211720 AT2G19400.1 51.786 56 27 0 31 86 409 464 1.68e-12 62.8
Msa1211720 AT5G09890.1 43.750 64 36 0 29 92 397 460 4.46e-11 58.9
Msa1211720 AT5G09890.2 43.750 64 36 0 29 92 397 460 4.47e-11 58.9

Find 46 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAGATTCTATTCCTTAAA+AGG 0.201477 8_1:-71467363 Msa1211720:three_prime_UTR
AGAAAGAGCAATTCATTTCT+TGG 0.212059 8_1:-71467725 Msa1211720:three_prime_UTR
TTTAGTACAAATTTCATTGT+AGG 0.302422 8_1:-71467644 Msa1211720:three_prime_UTR
AAGTTAAGCAAAACCATAAA+AGG 0.318810 8_1:-71467157 Msa1211720:three_prime_UTR
TTTAATTTGTGTACAGTGAT+TGG 0.319427 8_1:+71467310 None:intergenic
AGAGAAAGGTAAAGGACTTA+TGG 0.367920 8_1:-71470906 Msa1211720:CDS
TTGATTACACTAAAAGTGTC+TGG 0.376547 8_1:-71467236 Msa1211720:three_prime_UTR
CCTTCTTGAAATAATTCTGC+AGG 0.394500 8_1:-71466999 Msa1211720:three_prime_UTR
CTCATTCATCACCATTCTCA+TGG 0.407114 8_1:-71467045 Msa1211720:three_prime_UTR
TCTTTGACAGAAGGTGCGTT+TGG 0.417155 8_1:-71467998 Msa1211720:CDS
GGTTCTTCAAAGATGTTGAA+TGG 0.424378 8_1:-71469453 Msa1211720:CDS
CTTAGACTCAAAAGAGATAG+TGG 0.426474 8_1:+71467929 None:intergenic
GAGAAGTTATTCCATGAGAA+TGG 0.467919 8_1:+71467034 None:intergenic
CCTGCAGAATTATTTCAAGA+AGG 0.468444 8_1:+71466999 None:intergenic
GAAATGAATTGCTCTTTCTA+CGG 0.477666 8_1:+71467729 None:intergenic
CTCAAATAATTGATCATGTA+CGG 0.482023 8_1:+71467833 None:intergenic
ATTTCAGGCTATGAATCAAT+CGG 0.482997 8_1:-71468059 Msa1211720:intron
TTTAAAGAAAGAAAAGGAAA+AGG 0.483766 8_1:-71467464 Msa1211720:three_prime_UTR
GTTCTTCAAAGATGTTGAAT+GGG 0.484282 8_1:-71469452 Msa1211720:CDS
AGTGCAGATTTAGCCCATGC+TGG 0.489959 8_1:-71467578 Msa1211720:three_prime_UTR
GCATTCCCCTTCAGGCTCAT+CGG 0.496953 8_1:-71469474 Msa1211720:intron
TTTGTACCTGAGGTCAATGA+TGG 0.506108 8_1:-71469403 Msa1211720:CDS
CCTTCTGTCAAAGAGTACTT+AGG 0.508116 8_1:+71468007 None:intergenic
AGACAAGGACTTACAGAGAA+AGG 0.514463 8_1:-71470920 Msa1211720:CDS
CAAGGAAAGCAAGGAAGACA+AGG 0.522757 8_1:-71470935 Msa1211720:CDS
AAATCACTGTGCATAAAGAA+AGG 0.528749 8_1:-71467383 Msa1211720:three_prime_UTR
AAAAGCTGGTCCACGGAGGA+AGG 0.532164 8_1:-71469224 Msa1211720:intron
TCTATAGTTATTATGGAGCA+AGG 0.533021 8_1:-71470953 None:intergenic
GTTGTAGCTTGCAAGGAGAA+AGG 0.536980 8_1:-71470874 Msa1211720:CDS
CTTCTTGAAATAATTCTGCA+GGG 0.543153 8_1:-71466998 Msa1211720:three_prime_UTR
ACTCGACCGTCGTCAAAAGC+TGG 0.544275 8_1:-71469238 Msa1211720:CDS
CAAGGTGGTTGTAGCTTGCA+AGG 0.544564 8_1:-71470881 Msa1211720:CDS
ATTATGGAGCAAGGAAAGCA+AGG 0.553902 8_1:-71470944 Msa1211720:CDS
CAATTGTATGAATGTACGAC+AGG 0.562104 8_1:+71467673 None:intergenic
GTGCAGATTTAGCCCATGCT+GGG 0.563067 8_1:-71467577 Msa1211720:three_prime_UTR
TCTAATCCATCATTGACCTC+AGG 0.569047 8_1:+71469397 None:intergenic
GGACTTACAGAGAAAGGTAA+AGG 0.569274 8_1:-71470914 Msa1211720:CDS
AAGGATCAAGAAGAGATGAA+AGG 0.581058 8_1:-71467445 Msa1211720:three_prime_UTR
CCTAAGTACTCTTTGACAGA+AGG 0.601478 8_1:-71468007 Msa1211720:CDS
TGAAGCTGCATTTGTACCTG+AGG 0.608164 8_1:-71469413 Msa1211720:CDS
TGATTACACTAAAAGTGTCT+GGG 0.611357 8_1:-71467235 Msa1211720:three_prime_UTR
CGTCGTCAAAAGCTGGTCCA+CGG 0.611833 8_1:-71469231 Msa1211720:CDS
GGTAAAGGACTTATGGAGCA+AGG 0.644992 8_1:-71470899 Msa1211720:CDS
TCAAAGATTACCTTCCTCCG+TGG 0.688761 8_1:+71469214 None:intergenic
CGTCAAAAGCTGGTCCACGG+AGG 0.743654 8_1:-71469228 Msa1211720:CDS
AAAGGACTTATGGAGCAAGG+TGG 0.753797 8_1:-71470896 Msa1211720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATACTTAATGAATAAATT+TGG - chr8_1:71469662-71469681 Msa1211720:intron 10.0%
!!! ATAACATTTTATTCAATATA+GGG + chr8_1:71469743-71469762 None:intergenic 10.0%
!!! TTTTTTTTTAAGCTTTGATT+TGG - chr8_1:71467132-71467151 Msa1211720:three_prime_UTR 15.0%
!! AAATGATAGAAAAAAAAAAG+TGG - chr8_1:71467515-71467534 Msa1211720:three_prime_UTR 15.0%
!!! TTCTACTATTTTCATATAAT+TGG - chr8_1:71467638-71467657 Msa1211720:three_prime_UTR 15.0%
!! TATAAAAAAGAAAACAATGA+CGG + chr8_1:71467931-71467950 None:intergenic 15.0%
!!! TTTTTATATCTAATATGTGT+TGG - chr8_1:71467943-71467962 Msa1211720:three_prime_UTR 15.0%
!! CCTTAAAAAAAAATAAAAAG+AGG + chr8_1:71468092-71468111 None:intergenic 15.0%
!!! CCTCTTTTTATTTTTTTTTA+AGG - chr8_1:71468089-71468108 Msa1211720:intron 15.0%
!! AAAAAATATGTCAATTTCTT+TGG + chr8_1:71468234-71468253 None:intergenic 15.0%
!! ATATCTATACAAAATAAGAA+AGG + chr8_1:71468596-71468615 None:intergenic 15.0%
!!! GTACTTTTAAATTACTTATA+TGG - chr8_1:71468885-71468904 Msa1211720:intron 15.0%
!! AATTACTTATATGGAAATTA+TGG - chr8_1:71468894-71468913 Msa1211720:intron 15.0%
!! CAATAAATTTATTCATATCA+TGG + chr8_1:71469197-71469216 None:intergenic 15.0%
!! CATGATATGAATAAATTTAT+TGG - chr8_1:71469195-71469214 Msa1211720:intron 15.0%
!!! CATAACATTTTATTCAATAT+AGG + chr8_1:71469744-71469763 None:intergenic 15.0%
!!! TAATTTATTTGGTTTATTTC+AGG - chr8_1:71469846-71469865 Msa1211720:intron 15.0%
!!! AAAAATAATCTTTCCTTTTA+AGG + chr8_1:71470573-71470592 None:intergenic 15.0%
!!! ATCATTTTCTATCATTATAA+AGG - chr8_1:71470711-71470730 Msa1211720:intron 15.0%
!! AAAAAAAAAATAACAGGTAC+AGG + chr8_1:71467476-71467495 None:intergenic 20.0%
!!! TTTTTTTTTCTATCATTTGC+AGG + chr8_1:71467513-71467532 None:intergenic 20.0%
!! GCTACAAATACTAAAAAAAA+CGG + chr8_1:71467721-71467740 None:intergenic 20.0%
!! TATCTATTATAACTGCTTAA+AGG + chr8_1:71467743-71467762 None:intergenic 20.0%
!!! TAGTTTTAATGAACATACAT+TGG - chr8_1:71468156-71468175 Msa1211720:intron 20.0%
!!! AGTTTTAATGAACATACATT+GGG - chr8_1:71468157-71468176 Msa1211720:intron 20.0%
!! AATTTCTTTGGATAAGTATT+AGG + chr8_1:71468222-71468241 None:intergenic 20.0%
!! TTTCACCTATAAATAACTTA+AGG - chr8_1:71468252-71468271 Msa1211720:intron 20.0%
!! AGTTTCCTTAAGTTATTTAT+AGG + chr8_1:71468260-71468279 None:intergenic 20.0%
!! AAAAAATGTCATCTTTACAT+GGG + chr8_1:71468345-71468364 None:intergenic 20.0%
!! AAAGGTAAAATGACAAAAAA+TGG + chr8_1:71468639-71468658 None:intergenic 20.0%
!! CCTTATATTGCAAAAATTTA+TGG + chr8_1:71468832-71468851 None:intergenic 20.0%
!!! CCATAAATTTTTGCAATATA+AGG - chr8_1:71468829-71468848 Msa1211720:intron 20.0%
!! GGCAATAAAATTTCTTATAT+GGG + chr8_1:71469096-71469115 None:intergenic 20.0%
!! GAATAAATTTATTGGTATTG+CGG - chr8_1:71469203-71469222 Msa1211720:intron 20.0%
!!! AATTTTTGGTAAACGATTTT+TGG - chr8_1:71469314-71469333 Msa1211720:intron 20.0%
!! CTTATAGATTAATAAGCATA+AGG - chr8_1:71469491-71469510 Msa1211720:intron 20.0%
!! TTGTTAATCTTAGATCTTTA+TGG - chr8_1:71469521-71469540 Msa1211720:intron 20.0%
!! TGTTAATCTTAGATCTTTAT+GGG - chr8_1:71469522-71469541 Msa1211720:intron 20.0%
!! AAGAAATAAATCCATGTAAT+AGG - chr8_1:71469564-71469583 Msa1211720:intron 20.0%
!! CTTAATGAATAAATTTGGAA+TGG - chr8_1:71469667-71469686 Msa1211720:intron 20.0%
!! ATAAATTAGCATAAGGATAA+AGG + chr8_1:71469834-71469853 None:intergenic 20.0%
!! TATCCTTATGCTAATTTATT+TGG - chr8_1:71469835-71469854 Msa1211720:intron 20.0%
!! AAACCAAATAAATTAGCATA+AGG + chr8_1:71469841-71469860 None:intergenic 20.0%
!! TTTAGTACAAATTTCATTGT+AGG - chr8_1:71470276-71470295 Msa1211720:intron 20.0%
!!! GATTGTTTTAAAGAAAGAAA+AGG - chr8_1:71470450-71470469 Msa1211720:intron 20.0%
!! TTTAAAGAAAGAAAAGGAAA+AGG - chr8_1:71470456-71470475 Msa1211720:intron 20.0%
!!! TTTTAATACACATCCTTTTA+TGG + chr8_1:71470779-71470798 None:intergenic 20.0%
! TATGAGATATAAGTGCAAAT+TGG + chr8_1:71467183-71467202 None:intergenic 25.0%
! TTTATGTTCAGATAAACTTG+CGG + chr8_1:71467336-71467355 None:intergenic 25.0%
! AAAAAGTGGAGTTAAAAGAA+TGG - chr8_1:71467529-71467548 Msa1211720:three_prime_UTR 25.0%
! TTAAAAGAATGGTCAAAGAT+AGG - chr8_1:71467540-71467559 Msa1211720:three_prime_UTR 25.0%
! AGCAGTTATAATAGATATGA+TGG - chr8_1:71467746-71467765 Msa1211720:three_prime_UTR 25.0%
! GTAGTAAGAATGTTGTAATT+TGG - chr8_1:71467790-71467809 Msa1211720:three_prime_UTR 25.0%
! TGAAGAACACTAAAGTAAAA+AGG - chr8_1:71467830-71467849 Msa1211720:three_prime_UTR 25.0%
! TCATAAATAAGATTCCTGTA+TGG - chr8_1:71467859-71467878 Msa1211720:three_prime_UTR 25.0%
! AATGATGAAACAAAAACATG+TGG - chr8_1:71468045-71468064 Msa1211720:CDS 25.0%
! ATGATGAAACAAAAACATGT+GGG - chr8_1:71468046-71468065 Msa1211720:CDS 25.0%
!!! GTTTTAATGAACATACATTG+GGG - chr8_1:71468158-71468177 Msa1211720:intron 25.0%
! GAAAAAATGTCATCTTTACA+TGG + chr8_1:71468346-71468365 None:intergenic 25.0%
!!! GAATTTTGAAAAGTTTGATG+AGG - chr8_1:71468552-71468571 Msa1211720:intron 25.0%
! GTGTTTGAATAACCTATTAT+TGG - chr8_1:71468773-71468792 Msa1211720:intron 25.0%
! TCATCATATCAACCAATAAT+AGG + chr8_1:71468788-71468807 None:intergenic 25.0%
! GGGCAATAAAATTTCTTATA+TGG + chr8_1:71469097-71469116 None:intergenic 25.0%
! AAATCTCCCTAAAAACAAAA+AGG + chr8_1:71469121-71469140 None:intergenic 25.0%
! GTCATCAAATTATATTGATG+TGG - chr8_1:71469336-71469355 Msa1211720:intron 25.0%
! TCAAATTATATTGATGTGGT+TGG - chr8_1:71469340-71469359 Msa1211720:intron 25.0%
! TAGATCTTTATGGGATTATT+TGG - chr8_1:71469531-71469550 Msa1211720:intron 25.0%
! GTGAATTAAAAACCATTACA+AGG - chr8_1:71469598-71469617 Msa1211720:intron 25.0%
!! AAAAAGTGATTCTTCTTTTG+TGG - chr8_1:71469766-71469785 Msa1211720:intron 25.0%
!!! CTATTTCTTGACTTTTATTG+TGG - chr8_1:71469950-71469969 Msa1211720:intron 25.0%
! CTCAAATAATTGATCATGTA+CGG + chr8_1:71470090-71470109 None:intergenic 25.0%
!! GTGAAAGTTTTCTTCTTTAT+TGG - chr8_1:71470424-71470443 Msa1211720:intron 25.0%
!!! TTTAATTTGTGTACAGTGAT+TGG + chr8_1:71470613-71470632 None:intergenic 25.0%
! AAGTTAAGCAAAACCATAAA+AGG - chr8_1:71470763-71470782 Msa1211720:intron 25.0%
! TCTATAACTGAAGCAAAAAA+TGG - chr8_1:71470808-71470827 Msa1211720:intron 25.0%
TTACTTTAGTGTTCTTCACA+AGG + chr8_1:71467828-71467847 None:intergenic 30.0%
AAGAGGTAATAGATAGAAGA+GGG + chr8_1:71468075-71468094 None:intergenic 30.0%
AAAGAGGTAATAGATAGAAG+AGG + chr8_1:71468076-71468095 None:intergenic 30.0%
! TTTTTCTTCCATTTCAGCTA+TGG - chr8_1:71468360-71468379 Msa1211720:intron 30.0%
CAACATAACTATAACAAGAG+TGG + chr8_1:71468427-71468446 None:intergenic 30.0%
GTTCTTCAAAGATGTTGAAT+GGG - chr8_1:71468468-71468487 Msa1211720:intron 30.0%
TATAACTATAAACCACAGTC+AGG - chr8_1:71469022-71469041 Msa1211720:intron 30.0%
ATAACTATAAACCACAGTCA+GGG - chr8_1:71469023-71469042 Msa1211720:intron 30.0%
AGCTAGTGTGTATGTTAAAT+GGG - chr8_1:71469229-71469248 Msa1211720:CDS 30.0%
TAACCTGTTTCGTATGAAAA+GGG - chr8_1:71469253-71469272 Msa1211720:CDS 30.0%
TACCAAAAATTGAGACATTG+AGG + chr8_1:71469305-71469324 None:intergenic 30.0%
GTTCATAAATGCCTATTACA+TGG + chr8_1:71469578-71469597 None:intergenic 30.0%
TGTTTAACTCTACCTTGTAA+TGG + chr8_1:71469613-71469632 None:intergenic 30.0%
TCAATATAGGGTCAATTGTA+GGG + chr8_1:71469731-71469750 None:intergenic 30.0%
TTCAATATAGGGTCAATTGT+AGG + chr8_1:71469732-71469751 None:intergenic 30.0%
ATTTCAGGCTATGAATCAAT+CGG - chr8_1:71469861-71469880 Msa1211720:intron 30.0%
! TTTCTTGACTTTTATTGTGG+TGG - chr8_1:71469953-71469972 Msa1211720:intron 30.0%
GAAATGAATTGCTCTTTCTA+CGG + chr8_1:71470194-71470213 None:intergenic 30.0%
AGAAAGAGCAATTCATTTCT+TGG - chr8_1:71470195-71470214 Msa1211720:intron 30.0%
!!! AGCTCACATCTATTTTTTCA+TGG - chr8_1:71470222-71470241 Msa1211720:intron 30.0%
AAACAAAAATTTCCCAGCAT+GGG + chr8_1:71470358-71470377 None:intergenic 30.0%
AAAACAAAAATTTCCCAGCA+TGG + chr8_1:71470359-71470378 None:intergenic 30.0%
AAATCACTGTGCATAAAGAA+AGG - chr8_1:71470537-71470556 Msa1211720:intron 30.0%
AGGAGATTCTATTCCTTAAA+AGG - chr8_1:71470557-71470576 Msa1211720:intron 30.0%
!! TTGATTACACTAAAAGTGTC+TGG - chr8_1:71470684-71470703 Msa1211720:intron 30.0%
!! TGATTACACTAAAAGTGTCT+GGG - chr8_1:71470685-71470704 Msa1211720:intron 30.0%
AAAAAATGGAGTTCAAACAC+TGG - chr8_1:71470822-71470841 Msa1211720:intron 30.0%
CTTCTTGAAATAATTCTGCA+GGG - chr8_1:71470922-71470941 Msa1211720:CDS 30.0%
AGAGAAAGGTAAAGGACTTA+TGG - chr8_1:71467014-71467033 Msa1211720:three_prime_UTR 35.0%
ACGACTGTGAAAAATAGAGA+GGG + chr8_1:71467111-71467130 None:intergenic 35.0%
AACGACTGTGAAAAATAGAG+AGG + chr8_1:71467112-71467131 None:intergenic 35.0%
! ATTTGGAACAGAACCTTCAA+TGG - chr8_1:71467149-71467168 Msa1211720:three_prime_UTR 35.0%
ATAAGTGCAAATTGGAAGTC+TGG + chr8_1:71467175-71467194 None:intergenic 35.0%
AAAGAATGGTCAAAGATAGG+AGG - chr8_1:71467543-71467562 Msa1211720:three_prime_UTR 35.0%
ACAGTTGAGAGACTTTACTA+AGG - chr8_1:71467582-71467601 Msa1211720:three_prime_UTR 35.0%
ATAGTAGAAGCAACCTACTA+CGG + chr8_1:71467627-71467646 None:intergenic 35.0%
TCCTGTATGGAATTCATCAT+AGG - chr8_1:71467872-71467891 Msa1211720:three_prime_UTR 35.0%
TCCTATGATGAATTCCATAC+AGG + chr8_1:71467876-71467895 None:intergenic 35.0%
!! CAATGACGGATTATAGATCA+TGG + chr8_1:71467917-71467936 None:intergenic 35.0%
ATTTGTTTGTTTGCCAGCAA+TGG - chr8_1:71467969-71467988 Msa1211720:exon 35.0%
CTCAAAATCATATCCATTGC+TGG + chr8_1:71467985-71468004 None:intergenic 35.0%
!!! TTTTGAGTTTAGAGAGCTAG+AGG - chr8_1:71467998-71468017 Msa1211720:CDS 35.0%
ACACATTTCTATTGCATCCA+TGG - chr8_1:71468393-71468412 Msa1211720:intron 35.0%
AACTATAACAAGAGTGGAAG+AGG + chr8_1:71468421-71468440 None:intergenic 35.0%
GGTTCTTCAAAGATGTTGAA+TGG - chr8_1:71468467-71468486 Msa1211720:intron 35.0%
CACTAATTCGACAGATAACT+TGG + chr8_1:71468756-71468775 None:intergenic 35.0%
TATGCCTTGTAGTTGTTATG+AGG - chr8_1:71468979-71468998 Msa1211720:intron 35.0%
TTCACCTCATAACAACTACA+AGG + chr8_1:71468986-71469005 None:intergenic 35.0%
GTAGTTGTTATGAGGTGAAT+TGG - chr8_1:71468987-71469006 Msa1211720:intron 35.0%
GCAAGACATTTCCTACTATA+TGG + chr8_1:71469049-71469068 None:intergenic 35.0%
!!! TTGCCCTCCTTTTTGTTTTT+AGG - chr8_1:71469111-71469130 Msa1211720:intron 35.0%
!!! TGCCCTCCTTTTTGTTTTTA+GGG - chr8_1:71469112-71469131 Msa1211720:intron 35.0%
CTCCCTAAAAACAAAAAGGA+GGG + chr8_1:71469117-71469136 None:intergenic 35.0%
TCTCCCTAAAAACAAAAAGG+AGG + chr8_1:71469118-71469137 None:intergenic 35.0%
CAGCTAGTGTGTATGTTAAA+TGG - chr8_1:71469228-71469247 Msa1211720:CDS 35.0%
CTAACCTGTTTCGTATGAAA+AGG - chr8_1:71469252-71469271 Msa1211720:CDS 35.0%
CAAAAATTGAGACATTGAGG+TGG + chr8_1:71469302-71469321 None:intergenic 35.0%
! CACCTCAATGTCTCAATTTT+TGG - chr8_1:71469300-71469319 Msa1211720:intron 35.0%
TGATGTGGTTGGATATGTAT+TGG - chr8_1:71469351-71469370 Msa1211720:intron 35.0%
TCCTAACTTTCATTCTTGCA+CGG - chr8_1:71469418-71469437 Msa1211720:CDS 35.0%
GTTTGATCAAGAGTAAGAGA+AGG + chr8_1:71469709-71469728 None:intergenic 35.0%
CTTAGACTCAAAAGAGATAG+TGG + chr8_1:71469994-71470013 None:intergenic 35.0%
CAATTGTATGAATGTACGAC+AGG + chr8_1:71470250-71470269 None:intergenic 35.0%
AAGGATCAAGAAGAGATGAA+AGG - chr8_1:71470475-71470494 Msa1211720:intron 35.0%
GAGAAGTTATTCCATGAGAA+TGG + chr8_1:71470889-71470908 None:intergenic 35.0%
CCTGCAGAATTATTTCAAGA+AGG + chr8_1:71470924-71470943 None:intergenic 35.0%
CCTTCTTGAAATAATTCTGC+AGG - chr8_1:71470921-71470940 Msa1211720:CDS 35.0%
TCTGCAGGGAAAACAAAAAA+AGG - chr8_1:71470936-71470955 Msa1211720:CDS 35.0%
AGACAAGGACTTACAGAGAA+AGG - chr8_1:71467000-71467019 Msa1211720:three_prime_UTR 40.0%
GGACTTACAGAGAAAGGTAA+AGG - chr8_1:71467006-71467025 Msa1211720:three_prime_UTR 40.0%
CGACTGTGAAAAATAGAGAG+GGG + chr8_1:71467110-71467129 None:intergenic 40.0%
TGGTCAAAGATAGGAGGAAA+AGG - chr8_1:71467549-71467568 Msa1211720:three_prime_UTR 40.0%
CTTCCAAATACTACCGTAGT+AGG - chr8_1:71467611-71467630 Msa1211720:three_prime_UTR 40.0%
CAACCTACTACGGTAGTATT+TGG + chr8_1:71467617-71467636 None:intergenic 40.0%
GAGGAAGCAATTGTTATGCA+AGG - chr8_1:71468017-71468036 Msa1211720:CDS 40.0%
GAAATAATCCAATCCGAGAG+AGG + chr8_1:71468189-71468208 None:intergenic 40.0%
TCCATTTCAGCTATGGTGTA+TGG - chr8_1:71468367-71468386 Msa1211720:intron 40.0%
CCATTTCAGCTATGGTGTAT+GGG - chr8_1:71468368-71468387 Msa1211720:intron 40.0%
TTTGTACCTGAGGTCAATGA+TGG - chr8_1:71468517-71468536 Msa1211720:intron 40.0%
TCTAATCCATCATTGACCTC+AGG + chr8_1:71468526-71468545 None:intergenic 40.0%
! ATTTTACCTTTTGCACCTGC+AGG - chr8_1:71468648-71468667 Msa1211720:intron 40.0%
TGGTGAAAATATCCAGCAGA+GGG + chr8_1:71468736-71468755 None:intergenic 40.0%
TTGGTGAAAATATCCAGCAG+AGG + chr8_1:71468737-71468756 None:intergenic 40.0%
GTTGTTATGAGGTGAATTGG+TGG - chr8_1:71468990-71469009 Msa1211720:intron 40.0%
TCCGTGCAAGAATGAAAGTT+AGG + chr8_1:71469422-71469441 None:intergenic 40.0%
!! CCTTCTGTCAAAGAGTACTT+AGG + chr8_1:71469916-71469935 None:intergenic 40.0%
CCTAAGTACTCTTTGACAGA+AGG - chr8_1:71469913-71469932 Msa1211720:intron 40.0%
CTCATTCATCACCATTCTCA+TGG - chr8_1:71470875-71470894 Msa1211720:CDS 40.0%
CAAGGAAAGCAAGGAAGACA+AGG - chr8_1:71466985-71467004 Msa1211720:three_prime_UTR 45.0%
GGTAAAGGACTTATGGAGCA+AGG - chr8_1:71467021-71467040 Msa1211720:three_prime_UTR 45.0%
AAAGGACTTATGGAGCAAGG+TGG - chr8_1:71467024-71467043 Msa1211720:three_prime_UTR 45.0%
! GTTGTAGCTTGCAAGGAGAA+AGG - chr8_1:71467046-71467065 Msa1211720:three_prime_UTR 45.0%
ATTGGAAGTCTGGCCATTGA+AGG + chr8_1:71467165-71467184 None:intergenic 45.0%
! CATTGGGGTTTTTCCTCTCT+CGG - chr8_1:71468173-71468192 Msa1211720:intron 45.0%
CCCATACACCATAGCTGAAA+TGG + chr8_1:71468371-71468390 None:intergenic 45.0%
GTTATGTTGCATTCCCCTTC+AGG - chr8_1:71468438-71468457 Msa1211720:intron 45.0%
TGAAGCTGCATTTGTACCTG+AGG - chr8_1:71468507-71468526 Msa1211720:intron 45.0%
TCAAAGATTACCTTCCTCCG+TGG + chr8_1:71468709-71468728 None:intergenic 45.0%
ATCTTTGATGTTCCCTCTGC+TGG - chr8_1:71468721-71468740 Msa1211720:intron 45.0%
! CTGATGGACAAGCAAGTGAA+AGG + chr8_1:71468948-71468967 None:intergenic 45.0%
TCACTTGCTTGTCCATCAGT+AGG - chr8_1:71468949-71468968 Msa1211720:intron 45.0%
! AGGTGAATTGGTGGTTGAGA+AGG - chr8_1:71468999-71469018 Msa1211720:intron 45.0%
TGTCTTGCTAACTCTCGAAC+TGG - chr8_1:71469061-71469080 Msa1211720:intron 45.0%
! GCACCCTTTTCATACGAAAC+AGG + chr8_1:71469259-71469278 None:intergenic 45.0%
! TCTTTGACAGAAGGTGCGTT+TGG - chr8_1:71469922-71469941 Msa1211720:intron 45.0%
!! TATTAAAAAAAAAAAATAAC+AGG + chr8_1:71467482-71467501 None:intergenic 5.0%
! CAAGGTGGTTGTAGCTTGCA+AGG - chr8_1:71467039-71467058 Msa1211720:three_prime_UTR 50.0%
! GGGTTTTTCCTCTCTCGGAT+TGG - chr8_1:71468178-71468197 Msa1211720:intron 50.0%
!! CAAGAGTGGAAGAGGTACCA+TGG + chr8_1:71468413-71468432 None:intergenic 50.0%
AAGAACCGATGAGCCTGAAG+GGG + chr8_1:71468454-71468473 None:intergenic 50.0%
GAAGAACCGATGAGCCTGAA+GGG + chr8_1:71468455-71468474 None:intergenic 50.0%
TGAAGAACCGATGAGCCTGA+AGG + chr8_1:71468456-71468475 None:intergenic 50.0%
! CTTTTGACGACGGTCGAGTT+TGG + chr8_1:71468681-71468700 None:intergenic 50.0%
GCATATCAGCAGCCTACTGA+TGG + chr8_1:71468964-71468983 None:intergenic 50.0%
CTACTATATGGCCCTGACTG+TGG + chr8_1:71469037-71469056 None:intergenic 50.0%
CACAGTCAGGGCCATATAGT+AGG - chr8_1:71469035-71469054 Msa1211720:intron 50.0%
TGCTAACTCTCGAACTGGCT+CGG - chr8_1:71469066-71469085 Msa1211720:intron 50.0%
! GCATCGACGATGTCTCTATC+TGG + chr8_1:71469459-71469478 None:intergenic 50.0%
AGTGCAGATTTAGCCCATGC+TGG - chr8_1:71470342-71470361 Msa1211720:intron 50.0%
GTGCAGATTTAGCCCATGCT+GGG - chr8_1:71470343-71470362 Msa1211720:intron 50.0%
GCATTCCCCTTCAGGCTCAT+CGG - chr8_1:71468446-71468465 Msa1211720:intron 55.0%
TGTCAGCCTGCAGGTGCAAA+AGG + chr8_1:71468657-71468676 None:intergenic 55.0%
GAGTTTGGTTGTCAGCCTGC+AGG + chr8_1:71468666-71468685 None:intergenic 55.0%
ACTCGACCGTCGTCAAAAGC+TGG - chr8_1:71468682-71468701 Msa1211720:intron 55.0%
! CGTGGACCAGCTTTTGACGA+CGG + chr8_1:71468691-71468710 None:intergenic 55.0%
CGTCGTCAAAAGCTGGTCCA+CGG - chr8_1:71468689-71468708 Msa1211720:intron 55.0%
AAAAGCTGGTCCACGGAGGA+AGG - chr8_1:71468696-71468715 Msa1211720:intron 55.0%
! GCTAACTCTCGAACTGGCTC+GGG - chr8_1:71469067-71469086 Msa1211720:intron 55.0%
CGTCAAAAGCTGGTCCACGG+AGG - chr8_1:71468692-71468711 Msa1211720:intron 60.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 71466979 71470963 71466979 ID=Msa1211720;Name=Msa1211720
chr8_1 mRNA 71466979 71470963 71466979 ID=Msa1211720-mRNA-1;Parent=Msa1211720;Name=Msa1211720-mRNA-1;_AED=0.16;_eAED=0.21;_QI=0|0.33|0.25|1|1|1|4|1003|115
chr8_1 exon 71466979 71468074 71466979 ID=Msa1211720-mRNA-1:exon:24079;Parent=Msa1211720-mRNA-1
chr8_1 exon 71469225 71469272 71469225 ID=Msa1211720-mRNA-1:exon:24078;Parent=Msa1211720-mRNA-1
chr8_1 exon 71469369 71469482 71469369 ID=Msa1211720-mRNA-1:exon:24077;Parent=Msa1211720-mRNA-1
chr8_1 exon 71470871 71470963 71470871 ID=Msa1211720-mRNA-1:exon:24076;Parent=Msa1211720-mRNA-1
chr8_1 CDS 71470871 71470963 71470871 ID=Msa1211720-mRNA-1:cds;Parent=Msa1211720-mRNA-1
chr8_1 CDS 71469369 71469482 71469369 ID=Msa1211720-mRNA-1:cds;Parent=Msa1211720-mRNA-1
chr8_1 CDS 71469225 71469272 71469225 ID=Msa1211720-mRNA-1:cds;Parent=Msa1211720-mRNA-1
chr8_1 CDS 71467982 71468074 71467982 ID=Msa1211720-mRNA-1:cds;Parent=Msa1211720-mRNA-1
chr8_1 three_prime_UTR 71466979 71467981 71466979 ID=Msa1211720-mRNA-1:three_prime_utr;Parent=Msa1211720-mRNA-1
Gene Sequence

>Msa1211720

ATGGAGCAAGGAAAGCAAGGAAGACAAGGACTTACAGAGAAAGGTAAAGGACTTATGGAGCAAGGTGGTTGTAGCTTGCAAGGAGAAAGGCAAGCTCATCGGTTCTTCAAAGATGTTGAATGGGATAAACTATATCAAATTGAAGCTGCATTTGTACCTGAGGTCAATGATGGATTAGATACTATGAATTTTGAAAAGTTTGATGAGGCTGACAACCAAACTCGACCGTCGTCAAAAGCTGGTCCACGGAGGAAGGCTATGAATCAATCGGTGACTAAATCTTGTGAAATACGCGAAAAGCCTAAGTACTCTTTGACAGAAGGTGCGTTTGGCTTTTCTATTTCTTGA
Protein sequence

>Msa1211720

MEQGKQGRQGLTEKGKGLMEQGGCSLQGERQAHRFFKDVEWDKLYQIEAAFVPEVNDGLDTMNFEKFDEADNQTRPSSKAGPRRKAMNQSVTKSCEIREKPKYSLTEGAFGFSIS*