Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1216070 | A0A072UJT5 | 71.831 | 71 | 19 | 1 | 1 | 71 | 62 | 131 | 2.59e-27 | 106 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1216070 | Msa1301960 | 0.823107 | 1.654924e-53 | -8.615850e-47 |
| Msa0916390 | Msa1216070 | 0.876316 | 1.538539e-68 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTAATGAATTAAGATATT+TGG | 0.189007 | 8_1:+77646682 | Msa1216070:three_prime_UTR |
| TTCCACTTCATCTGGAATAT+TGG | 0.224732 | 8_1:-77646223 | None:intergenic |
| GAATTAAGATATTTGGCTAT+TGG | 0.308390 | 8_1:+77646689 | Msa1216070:three_prime_UTR |
| GATGATTTGCAAAACTTCAT+TGG | 0.315605 | 8_1:+77646198 | Msa1216070:CDS |
| GAGAGTTTATATTTCTCATT+TGG | 0.346364 | 8_1:+77646632 | Msa1216070:three_prime_UTR |
| GGAAGGATTTCCACTTCATC+TGG | 0.370932 | 8_1:-77646231 | None:intergenic |
| ATGGGAGTTCTAAGCAAAAC+AGG | 0.442206 | 8_1:+77646172 | Msa1216070:CDS |
| GTACACTCTTTGTATAATGC+AGG | 0.480888 | 8_1:+77646711 | Msa1216070:three_prime_UTR |
| CTTGAAAGTAACATGGAAGA+TGG | 0.491264 | 8_1:+77646565 | Msa1216070:three_prime_UTR |
| ATCCAATATTCCAGATGAAG+TGG | 0.548791 | 8_1:+77646221 | Msa1216070:CDS |
| TATGCAAATACTAAAGGTAG+TGG | 0.550500 | 8_1:+77646261 | Msa1216070:CDS |
| TATTTGCATACTGTGGTGGA+AGG | 0.554728 | 8_1:-77646248 | None:intergenic |
| CCACAGTATGCAAATACTAA+AGG | 0.557310 | 8_1:+77646255 | Msa1216070:CDS |
| AATCAAAAGTGGCAAAGAGA+AGG | 0.585280 | 8_1:+77646290 | Msa1216070:CDS |
| TTAGTATTTGCATACTGTGG+TGG | 0.594473 | 8_1:-77646252 | None:intergenic |
| AGTGGTAAAAGAATCAAAAG+TGG | 0.595935 | 8_1:+77646279 | Msa1216070:CDS |
| CCTTTAGTATTTGCATACTG+TGG | 0.599993 | 8_1:-77646255 | None:intergenic |
| TGAATGACTTGAAAGTAACA+TGG | 0.656385 | 8_1:+77646558 | Msa1216070:exon |
| ACGAGACTGTGCAAGGCATG+CGG | 0.664920 | 8_1:+77646336 | Msa1216070:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTTTTTTTTTTGTTGA+AGG | + | chr8_1:77646592-77646611 | Msa1216070:three_prime_UTR | 15.0% |
| !!! | AACATTGGAGATTATTTTTT+AGG | - | chr8_1:77646393-77646412 | None:intergenic | 20.0% |
| !!! | AAAAATAATCTCCAATGTTT+TGG | + | chr8_1:77646394-77646413 | Msa1216070:intron | 20.0% |
| !!! | TTTTTCAATTACCAAAACAT+TGG | - | chr8_1:77646408-77646427 | None:intergenic | 20.0% |
| !!! | AAAACTTTAGTTTATGTTTC+TGG | + | chr8_1:77646489-77646508 | Msa1216070:intron | 20.0% |
| !! | GTGTAATGAATTAAGATATT+TGG | + | chr8_1:77646682-77646701 | Msa1216070:three_prime_UTR | 20.0% |
| !!! | ATTATTTTTTAGGACAGTTG+CGG | - | chr8_1:77646383-77646402 | None:intergenic | 25.0% |
| !!! | TAGTTTATGTTTCTGGTTTT+AGG | + | chr8_1:77646496-77646515 | Msa1216070:intron | 25.0% |
| ! | GAGAGTTTATATTTCTCATT+TGG | + | chr8_1:77646632-77646651 | Msa1216070:three_prime_UTR | 25.0% |
| ! | GAATTAAGATATTTGGCTAT+TGG | + | chr8_1:77646689-77646708 | Msa1216070:three_prime_UTR | 25.0% |
| ! | AAACAGTTGGATGATTTTGA+TGG | + | chr8_1:77646153-77646172 | Msa1216070:CDS | 30.0% |
| !! | AACAGTTGGATGATTTTGAT+GGG | + | chr8_1:77646154-77646173 | Msa1216070:CDS | 30.0% |
| ! | GATGATTTGCAAAACTTCAT+TGG | + | chr8_1:77646198-77646217 | Msa1216070:CDS | 30.0% |
| TATGCAAATACTAAAGGTAG+TGG | + | chr8_1:77646261-77646280 | Msa1216070:CDS | 30.0% | |
| AGTGGTAAAAGAATCAAAAG+TGG | + | chr8_1:77646279-77646298 | Msa1216070:CDS | 30.0% | |
| !!! | TTTCTGGTTTTAGGTTTTGA+AGG | + | chr8_1:77646505-77646524 | Msa1216070:intron | 30.0% |
| TGAATGACTTGAAAGTAACA+TGG | + | chr8_1:77646558-77646577 | Msa1216070:exon | 30.0% | |
| !!! | GAAGGAAGAGCTATTTTTTA+TGG | + | chr8_1:77646610-77646629 | Msa1216070:three_prime_UTR | 30.0% |
| !!! | TCAGTGTACTTGTTTTGATT+TGG | + | chr8_1:77646660-77646679 | Msa1216070:three_prime_UTR | 30.0% |
| ATCCAATATTCCAGATGAAG+TGG | + | chr8_1:77646221-77646240 | Msa1216070:CDS | 35.0% | |
| ! | TTCCACTTCATCTGGAATAT+TGG | - | chr8_1:77646226-77646245 | None:intergenic | 35.0% |
| TTAGTATTTGCATACTGTGG+TGG | - | chr8_1:77646255-77646274 | None:intergenic | 35.0% | |
| CCTTTAGTATTTGCATACTG+TGG | - | chr8_1:77646258-77646277 | None:intergenic | 35.0% | |
| CCACAGTATGCAAATACTAA+AGG | + | chr8_1:77646255-77646274 | Msa1216070:CDS | 35.0% | |
| AATCAAAAGTGGCAAAGAGA+AGG | + | chr8_1:77646290-77646309 | Msa1216070:CDS | 35.0% | |
| CTTGAAAGTAACATGGAAGA+TGG | + | chr8_1:77646565-77646584 | Msa1216070:three_prime_UTR | 35.0% | |
| GTACACTCTTTGTATAATGC+AGG | + | chr8_1:77646711-77646730 | Msa1216070:three_prime_UTR | 35.0% | |
| !! | ATGGGAGTTCTAAGCAAAAC+AGG | + | chr8_1:77646172-77646191 | Msa1216070:CDS | 40.0% |
| ! | TATTTGCATACTGTGGTGGA+AGG | - | chr8_1:77646251-77646270 | None:intergenic | 40.0% |
| GGAAGGATTTCCACTTCATC+TGG | - | chr8_1:77646234-77646253 | None:intergenic | 45.0% | |
| GAAAAAGACGAGACTGTGCA+AGG | + | chr8_1:77646329-77646348 | Msa1216070:CDS | 45.0% | |
| ACGAGACTGTGCAAGGCATG+CGG | + | chr8_1:77646336-77646355 | Msa1216070:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 77646147 | 77646757 | 77646147 | ID=Msa1216070;Name=Msa1216070 |
| chr8_1 | mRNA | 77646147 | 77646757 | 77646147 | ID=Msa1216070-mRNA-1;Parent=Msa1216070;Name=Msa1216070-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0.5|1|1|1|2|193|85 |
| chr8_1 | exon | 77646147 | 77646357 | 77646147 | ID=Msa1216070-mRNA-1:exon:26836;Parent=Msa1216070-mRNA-1 |
| chr8_1 | exon | 77646518 | 77646757 | 77646518 | ID=Msa1216070-mRNA-1:exon:26837;Parent=Msa1216070-mRNA-1 |
| chr8_1 | CDS | 77646147 | 77646357 | 77646147 | ID=Msa1216070-mRNA-1:cds;Parent=Msa1216070-mRNA-1 |
| chr8_1 | CDS | 77646518 | 77646564 | 77646518 | ID=Msa1216070-mRNA-1:cds;Parent=Msa1216070-mRNA-1 |
| chr8_1 | three_prime_UTR | 77646565 | 77646757 | 77646565 | ID=Msa1216070-mRNA-1:three_prime_utr;Parent=Msa1216070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
