Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0916390 | XP_024642367.1 | 76.056 | 71 | 17 | 0 | 1 | 71 | 123 | 193 | 2.61e-31 | 119 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014710 | Msa0916390 | 0.828336 | 9.516599e-55 | -8.615850e-47 |
| Msa0020410 | Msa0916390 | 0.803901 | 2.794339e-49 | -8.615850e-47 |
| Msa0021210 | Msa0916390 | 0.802180 | 6.339670e-49 | -8.615850e-47 |
| Msa0114430 | Msa0916390 | 0.800389 | 1.474900e-48 | -8.615850e-47 |
| Msa0120340 | Msa0916390 | 0.809269 | 2.057260e-50 | -8.615850e-47 |
| Msa0620960 | Msa0916390 | 0.821353 | 4.225213e-53 | -8.615850e-47 |
| Msa0626220 | Msa0916390 | 0.817214 | 3.703407e-52 | -8.615850e-47 |
| Msa0777230 | Msa0916390 | 0.806723 | 7.163489e-50 | -8.615850e-47 |
| Msa0791240 | Msa0916390 | 0.811505 | 6.768951e-51 | -8.615850e-47 |
| Msa0820850 | Msa0916390 | 0.805546 | 1.267329e-49 | -8.615850e-47 |
| Msa0405370 | Msa0916390 | 0.853331 | 2.565398e-61 | -8.615850e-47 |
| Msa0303150 | Msa0916390 | 0.802340 | 5.877230e-49 | -8.615850e-47 |
| Msa0359030 | Msa0916390 | 0.800767 | 1.234792e-48 | -8.615850e-47 |
| Msa0860030 | Msa0916390 | -0.817726 | 2.840066e-52 | -8.615850e-47 |
| Msa0861130 | Msa0916390 | 0.803537 | 3.324700e-49 | -8.615850e-47 |
| Msa0864470 | Msa0916390 | -0.815571 | 8.633498e-52 | -8.615850e-47 |
| Msa0916390 | Msa1082500 | 0.808443 | 3.089312e-50 | -8.615850e-47 |
| Msa0916390 | Msa1216070 | 0.876316 | 1.538539e-68 | -8.615850e-47 |
| Msa0916390 | Msa1233890 | 0.848152 | 7.350765e-60 | -8.615850e-47 |
| Msa0916390 | Msa1237980 | 0.800119 | 1.673604e-48 | -8.615850e-47 |
| Msa0916390 | Msa1301960 | 0.845713 | 3.419535e-59 | -8.615850e-47 |
| Msa0916390 | Msa1410100 | 0.805497 | 1.297346e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTAATGAATTAAGATATT+TGG | 0.189007 | 6_2:+36324465 | Msa0916390:three_prime_UTR |
| TTCCACTTCATCTGGAATAT+TGG | 0.224732 | 6_2:-36324004 | None:intergenic |
| GAATTAAGATATTTGGCTAT+TGG | 0.308390 | 6_2:+36324472 | Msa0916390:three_prime_UTR |
| GAGAGTTTATATTTCTCATT+TGG | 0.346364 | 6_2:+36324415 | Msa0916390:three_prime_UTR |
| GGAAGGATTTCCACTTCATC+TGG | 0.370932 | 6_2:-36324012 | None:intergenic |
| ATGGGAGTTCTAAGCAAAAC+AGG | 0.442206 | 6_2:+36323953 | Msa0916390:CDS |
| CCTTTAGTATTTGCATATTG+TGG | 0.479904 | 6_2:-36324036 | None:intergenic |
| CTTGAAAGTAACATGGAAGA+TGG | 0.491264 | 6_2:+36324346 | Msa0916390:three_prime_UTR |
| GATGATTTGCAAAACTTCAT+CGG | 0.510032 | 6_2:+36323979 | Msa0916390:CDS |
| TATTTGCATATTGTGGTGGA+AGG | 0.535502 | 6_2:-36324029 | None:intergenic |
| ATCCAATATTCCAGATGAAG+TGG | 0.544557 | 6_2:+36324002 | Msa0916390:CDS |
| CCACAATATGCAAATACTAA+AGG | 0.550490 | 6_2:+36324036 | Msa0916390:CDS |
| TATGCAAATACTAAAGGTAG+TGG | 0.550500 | 6_2:+36324042 | Msa0916390:CDS |
| AATCAAAAGTGGCAAAGAGA+AGG | 0.585280 | 6_2:+36324071 | Msa0916390:CDS |
| TTAGTATTTGCATATTGTGG+TGG | 0.586973 | 6_2:-36324033 | None:intergenic |
| AGTGGTAAAAGAATCAAAAG+TGG | 0.595935 | 6_2:+36324060 | Msa0916390:CDS |
| ACGAGACTGTGCAAGGCTTG+CGG | 0.628515 | 6_2:+36324117 | Msa0916390:CDS |
| TGAATGACTTGAAAGTAACA+TGG | 0.656385 | 6_2:+36324339 | Msa0916390:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTTTTGTTGA+AGG | + | chr6_2:36324375-36324394 | Msa0916390:three_prime_UTR | 10.0% |
| !!! | AACATTGGAGATTATTTTTT+AGG | - | chr6_2:36324174-36324193 | None:intergenic | 20.0% |
| !!! | AAAAATAATCTCCAATGTTT+TGG | + | chr6_2:36324175-36324194 | Msa0916390:intron | 20.0% |
| !!! | TTTTTCAATTACCAAAACAT+TGG | - | chr6_2:36324189-36324208 | None:intergenic | 20.0% |
| !!! | AAAACTTTAGTTTATGTTTC+TGG | + | chr6_2:36324270-36324289 | Msa0916390:intron | 20.0% |
| !! | GTGTAATGAATTAAGATATT+TGG | + | chr6_2:36324465-36324484 | Msa0916390:three_prime_UTR | 20.0% |
| !!! | ATTATTTTTTAGGACAGTTG+CGG | - | chr6_2:36324164-36324183 | None:intergenic | 25.0% |
| !!! | TAGTTTATGTTTCTGGTTTT+AGG | + | chr6_2:36324277-36324296 | Msa0916390:intron | 25.0% |
| ! | GAGAGTTTATATTTCTCATT+TGG | + | chr6_2:36324415-36324434 | Msa0916390:three_prime_UTR | 25.0% |
| ! | GAATTAAGATATTTGGCTAT+TGG | + | chr6_2:36324472-36324491 | Msa0916390:three_prime_UTR | 25.0% |
| ! | AAACAGTTGGATGATTTTGA+TGG | + | chr6_2:36323934-36323953 | Msa0916390:CDS | 30.0% |
| !! | AACAGTTGGATGATTTTGAT+GGG | + | chr6_2:36323935-36323954 | Msa0916390:CDS | 30.0% |
| ! | GATGATTTGCAAAACTTCAT+CGG | + | chr6_2:36323979-36323998 | Msa0916390:CDS | 30.0% |
| TTAGTATTTGCATATTGTGG+TGG | - | chr6_2:36324036-36324055 | None:intergenic | 30.0% | |
| CCTTTAGTATTTGCATATTG+TGG | - | chr6_2:36324039-36324058 | None:intergenic | 30.0% | |
| CCACAATATGCAAATACTAA+AGG | + | chr6_2:36324036-36324055 | Msa0916390:CDS | 30.0% | |
| TATGCAAATACTAAAGGTAG+TGG | + | chr6_2:36324042-36324061 | Msa0916390:CDS | 30.0% | |
| AGTGGTAAAAGAATCAAAAG+TGG | + | chr6_2:36324060-36324079 | Msa0916390:CDS | 30.0% | |
| !!! | TTTCTGGTTTTAGGTTTTGA+AGG | + | chr6_2:36324286-36324305 | Msa0916390:intron | 30.0% |
| TGAATGACTTGAAAGTAACA+TGG | + | chr6_2:36324339-36324358 | Msa0916390:exon | 30.0% | |
| !!! | GAAGGAAGAGCTATTTTTTA+TGG | + | chr6_2:36324393-36324412 | Msa0916390:three_prime_UTR | 30.0% |
| !!! | TCAGTGTACTTGTTTTGATT+TGG | + | chr6_2:36324443-36324462 | Msa0916390:three_prime_UTR | 30.0% |
| ATCCAATATTCCAGATGAAG+TGG | + | chr6_2:36324002-36324021 | Msa0916390:CDS | 35.0% | |
| ! | TTCCACTTCATCTGGAATAT+TGG | - | chr6_2:36324007-36324026 | None:intergenic | 35.0% |
| ! | TATTTGCATATTGTGGTGGA+AGG | - | chr6_2:36324032-36324051 | None:intergenic | 35.0% |
| AATCAAAAGTGGCAAAGAGA+AGG | + | chr6_2:36324071-36324090 | Msa0916390:CDS | 35.0% | |
| CTTGAAAGTAACATGGAAGA+TGG | + | chr6_2:36324346-36324365 | Msa0916390:three_prime_UTR | 35.0% | |
| !! | ATGGGAGTTCTAAGCAAAAC+AGG | + | chr6_2:36323953-36323972 | Msa0916390:CDS | 40.0% |
| GGAAGGATTTCCACTTCATC+TGG | - | chr6_2:36324015-36324034 | None:intergenic | 45.0% | |
| GAAAAAGACGAGACTGTGCA+AGG | + | chr6_2:36324110-36324129 | Msa0916390:CDS | 45.0% | |
| ACGAGACTGTGCAAGGCTTG+CGG | + | chr6_2:36324117-36324136 | Msa0916390:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 36323928 | 36324540 | 36323928 | ID=Msa0916390;Name=Msa0916390 |
| chr6_2 | mRNA | 36323928 | 36324540 | 36323928 | ID=Msa0916390-mRNA-1;Parent=Msa0916390;Name=Msa0916390-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0.5|1|1|1|2|195|85 |
| chr6_2 | exon | 36323928 | 36324138 | 36323928 | ID=Msa0916390-mRNA-1:exon:6296;Parent=Msa0916390-mRNA-1 |
| chr6_2 | exon | 36324299 | 36324540 | 36324299 | ID=Msa0916390-mRNA-1:exon:6297;Parent=Msa0916390-mRNA-1 |
| chr6_2 | CDS | 36323928 | 36324138 | 36323928 | ID=Msa0916390-mRNA-1:cds;Parent=Msa0916390-mRNA-1 |
| chr6_2 | CDS | 36324299 | 36324345 | 36324299 | ID=Msa0916390-mRNA-1:cds;Parent=Msa0916390-mRNA-1 |
| chr6_2 | three_prime_UTR | 36324346 | 36324540 | 36324346 | ID=Msa0916390-mRNA-1:three_prime_utr;Parent=Msa0916390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
