AlfalfaGEDB | Msa1216240

M. sativa cultivar zhongmu-4 / Msa1216240

KEGG: K01061
GO: GO:0005575 GO:0005576
PFAM: PF01738.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1216240	XP_013458180.1	96.266	241	7	1	1	241	55	293	2.49e-166	471

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1216240	sp\|Q9ZT66\|E134_MAIZE	44.379	169	90	2	28	196	55	219	6.79e-45	155

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1216240	Msa1216490	0.890897	6.503018e-74	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1216240	MtrunA17_Chr4g0065821	78.008	241	46	2	1	241	1	234	1.71e-134	378
Msa1216240	MtrunA17_Chr4g0065781	66.805	241	77	2	1	241	1	238	6.62e-109	313
Msa1216240	MtrunA17_Chr4g0065811	97.260	146	4	0	96	241	1	146	3.72e-100	288
Msa1216240	MtrunA17_Chr4g0065791	58.506	241	85	2	1	241	1	226	7.77e-97	282
Msa1216240	MtrunA17_Chr2g0287281	52.466	223	105	1	20	241	22	244	8.71e-78	234
Msa1216240	MtrunA17_Chr4g0065801	94.231	52	1	1	1	52	55	104	5.75e-27	100
Msa1216240	MtrunA17_Chr3g0077171	27.184	206	136	5	43	240	133	332	4.74e-13	67.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1216240	AT3G23600.1	63.071	241	87	1	1	241	1	239	5.79e-115	329
Msa1216240	AT3G23600.2	62.241	241	86	2	1	241	1	236	1.74e-111	320
Msa1216240	AT3G23570.3	58.921	241	97	1	1	241	1	239	2.33e-109	315
Msa1216240	AT3G23570.1	58.921	241	97	1	1	241	1	239	2.33e-109	315
Msa1216240	AT3G23570.2	58.921	241	97	1	1	241	1	239	2.33e-109	315
Msa1216240	AT1G35420.3	24.091	220	151	6	27	236	56	269	6.20e-18	80.9
Msa1216240	AT1G35420.1	24.091	220	151	6	27	236	92	305	9.42e-18	80.9
Msa1216240	AT1G35420.2	24.091	220	151	6	27	236	97	310	1.05e-17	80.9

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Find 60 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAGAGAATGGCTTTGG+TGG	0.467774	8_1:-77877226	None:intergenic
AATATTCCAATTGCTATACT+TGG	0.381640	8_1:+77879080	Msa1216240:CDS
AATTGTTTAATGACCTCTGG+AGG	0.570776	8_1:-77879125	None:intergenic
ACCAATCCAGCAAGATTTGA+TGG	0.410123	8_1:-77879712	None:intergenic
ACGAAGAGCTCATAGAGTGG+AGG	0.675987	8_1:+77879768	Msa1216240:three_prime_UTR
ACTAAATAATTCAGAATATA+AGG	0.268465	8_1:-77879898	None:intergenic
ACTCATTCAAGCTGTTGTGT+TGG	0.565853	8_1:+77878469	Msa1216240:CDS
AGAAACAAAGAGAATGGCTT+TGG	0.446253	8_1:-77877229	None:intergenic
AGAATGGCTTTGGTGGAAAG+AGG	0.487874	8_1:-77877219	None:intergenic
AGAGGCCCATCAAATCTTGC+TGG	0.465174	8_1:+77879706	Msa1216240:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTCAAAGAAACAAAAATGTC+AGG	+	chr8_1:77877093-77877112	Msa1216240:exon	30.0%
TAAAAGAGTTCAGAATTGGT+GGG	-	chr8_1:77877143-77877162	None:intergenic	30.0%
GATATCAGAAACAAAGAGAA+TGG	-	chr8_1:77877238-77877257	None:intergenic	30.0%
GATATGAAGCTCCAATTATA+AGG	+	chr8_1:77877386-77877405	Msa1216240:CDS	30.0%
ATATAAAGTCAACAAGGATG+TGG	-	chr8_1:77877521-77877540	None:intergenic	30.0%
CTACTAAGGATAGCAAAAAT+TGG	-	chr8_1:77877843-77877862	None:intergenic	30.0%
ATTACACTTGCCACATTTAT+AGG	+	chr8_1:77878055-77878074	Msa1216240:intron	30.0%
ATTGAGGCCTTAAAAAGTAA+AGG	+	chr8_1:77878227-77878246	Msa1216240:CDS	30.0%
GAAAACTGCTATATACACAA+AGG	-	chr8_1:77878326-77878345	None:intergenic	30.0%
CATTAAGCTAACACATCTTA+TGG	-	chr8_1:77878765-77878784	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	77877075	77879917	77877075	ID=Msa1216240;Name=Msa1216240
chr8_1	mRNA	77877075	77879917	77877075	ID=Msa1216240-mRNA-1;Parent=Msa1216240;Name=Msa1216240-mRNA-1;_AED=0.44;_eAED=0.44;_QI=33\|1\|1\|1\|1\|1\|7\|163\|241
chr8_1	exon	77877075	77877261	77877075	ID=Msa1216240-mRNA-1:exon:26949;Parent=Msa1216240-mRNA-1
chr8_1	exon	77877386	77877407	77877386	ID=Msa1216240-mRNA-1:exon:26950;Parent=Msa1216240-mRNA-1
chr8_1	exon	77877635	77877761	77877635	ID=Msa1216240-mRNA-1:exon:26951;Parent=Msa1216240-mRNA-1
chr8_1	exon	77878197	77878287	77878197	ID=Msa1216240-mRNA-1:exon:26952;Parent=Msa1216240-mRNA-1
chr8_1	exon	77878438	77878527	77878438	ID=Msa1216240-mRNA-1:exon:26953;Parent=Msa1216240-mRNA-1
chr8_1	exon	77879075	77879175	77879075	ID=Msa1216240-mRNA-1:exon:26954;Parent=Msa1216240-mRNA-1
chr8_1	exon	77879614	77879917	77879614	ID=Msa1216240-mRNA-1:exon:26955;Parent=Msa1216240-mRNA-1
chr8_1	five_prime_UTR	77877075	77877107	77877075	ID=Msa1216240-mRNA-1:five_prime_utr;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77877108	77877261	77877108	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77877386	77877407	77877386	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77877635	77877761	77877635	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77878197	77878287	77878197	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77878438	77878527	77878438	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77879075	77879175	77879075	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	CDS	77879614	77879754	77879614	ID=Msa1216240-mRNA-1:cds;Parent=Msa1216240-mRNA-1
chr8_1	three_prime_UTR	77879755	77879917	77879755	ID=Msa1216240-mRNA-1:three_prime_utr;Parent=Msa1216240-mRNA-1

Gene Sequence

>Msa1216240

ATGTCAGGTCATGCATGCTGCTCTAAGTCTAACCCACCAATTCTGAACTCTTTTAGTGGAGCTGGCCATGTTGAAAAAATTGGTGGTCTTGATGCTTATCTCACTGGCTCTCCTCTTTCCACCAAAGCCATTCTCTTTGTTTCTGATATCTATGGATATGAAGCTCCAATTATAAGGAATCTTGCAGACAAGGTTGCAGCAGCTGGATATTATGTGGTAGTTCCTGACTACTTCTACGGCGATCCCTATGATGATGACTGTGTTGACAGGCCTTTACCTATTTGGATAAAAGACCATGAACCGGACAAAGGTTTTGAAGCTTCACAACCCATAATTGAGGCCTTAAAAAGTAAAGGTGTTTCTGCTATTGGAGCTGCTGGTTATTGTTGGGGTGCTAAGACTGTGTGTGAACTTGGGAAATCCAAACTCATTCAAGCTGTTGTGTTGGCACATCCATCATCTATCACAGTAGAGGACATCAATGGTGTTAATATTCCAATTGCTATACTTGGAGCTGAGCTTGATCCAATTACTCCTCCAGAGGTCATTAAACAATTTGAACAAGTCTTGGCTGCTAAACCTGATGTTGATAGTTTTGTAAAAATATTTCCTAATGTCTCGCATGGCTGGACTATAAGGTATGACACTGAAGATCCAGAAGCTGTGAAGGCTGCAGAAGAGGCCCATCAAATCTTGCTGGATTGGTTTGTTAAATATGTTAAGTGA

Protein sequence

>Msa1216240

MSGHACCSKSNPPILNSFSGAGHVEKIGGLDAYLTGSPLSTKAILFVSDIYGYEAPIIRNLADKVAAAGYYVVVPDYFYGDPYDDDCVDRPLPIWIKDHEPDKGFEASQPIIEALKSKGVSAIGAAGYCWGAKTVCELGKSKLIQAVVLAHPSSITVEDINGVNIPIAILGAELDPITPPEVIKQFEQVLAAKPDVDSFVKIFPNVSHGWTIRYDTEDPEAVKAAEEAHQILLDWFVKYVK*