AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216430 MtrunA17_Chr4g0065741 90.798 163 13 2 1 162 1 162 1.58e-96 275
Msa1216430 MtrunA17_Chr3g0088511 39.130 92 56 0 70 161 31 122 6.58e-19 77.8
Msa1216430 MtrunA17_Chr1g0198431 44.565 92 50 1 57 148 179 269 2.36e-16 74.3
Msa1216430 MtrunA17_Chr6g0481071 38.272 81 50 0 71 151 95 175 6.92e-16 71.2
Msa1216430 MtrunA17_Chr4g0031091 34.906 106 60 1 45 150 16 112 8.32e-16 69.3
Msa1216430 MtrunA17_Chr7g0263001 38.462 104 61 2 47 149 12 113 1.58e-15 69.3
Msa1216430 MtrunA17_Chr4g0061451 39.286 84 50 1 65 148 37 119 1.98e-15 71.6
Msa1216430 MtrunA17_Chr4g0035031 41.558 77 45 0 72 148 9 85 3.42e-15 69.7
Msa1216430 MtrunA17_Chr7g0219021 40.698 86 47 1 69 150 38 123 5.36e-15 70.1
Msa1216430 MtrunA17_Chr7g0266751 34.524 84 55 0 72 155 33 116 7.14e-15 67.4
Msa1216430 MtrunA17_Chr3g0119311 38.961 77 47 0 71 147 36 112 7.18e-15 67.4
Msa1216430 MtrunA17_Chr3g0121631 38.272 81 49 1 69 149 34 113 5.02e-14 65.5
Msa1216430 MtrunA17_Chr7g0259641 40.230 87 52 0 72 158 274 360 1.30e-13 67.4
Msa1216430 MtrunA17_Chr4g0035051 37.662 77 48 0 72 148 9 85 1.45e-13 63.5
Msa1216430 MtrunA17_Chr4g0035001 37.662 77 48 0 72 148 7 83 5.33e-13 63.2
Msa1216430 MtrunA17_Chr7g0259671 40.230 87 52 0 72 158 280 366 7.84e-13 65.1
Msa1216430 MtrunA17_Chr3g0086861 34.146 82 53 1 67 148 41 121 8.30e-13 65.1
Msa1216430 MtrunA17_Chr4g0050781 37.179 78 49 0 72 149 8 85 1.09e-12 63.2
Msa1216430 MtrunA17_Chr2g0327921 36.585 82 52 0 71 152 7 88 1.71e-12 60.1
Msa1216430 MtrunA17_Chr4g0042151 35.065 77 50 0 73 149 46 122 3.93e-12 61.2
Msa1216430 MtrunA17_Chr2g0278661 37.179 78 49 0 72 149 8 85 5.50e-12 61.6
Msa1216430 MtrunA17_Chr5g0430471 36.782 87 55 0 62 148 30 116 1.00e-11 59.3
Msa1216430 MtrunA17_Chr7g0260761 42.857 77 44 0 72 148 278 354 6.09e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216430 AT5G19030.1 53.012 83 39 0 74 156 80 162 1.19e-25 96.7
Msa1216430 AT4G20030.1 43.529 85 48 0 66 150 35 119 6.31e-20 81.3
Msa1216430 AT5G19030.5 53.846 65 30 0 74 138 80 144 3.38e-19 79.3
Msa1216430 AT5G19030.2 54.839 62 28 0 74 135 80 141 5.90e-18 75.9
Msa1216430 AT3G23830.2 43.373 83 47 0 70 152 34 116 1.16e-17 75.1
Msa1216430 AT3G23830.1 43.373 83 47 0 70 152 34 116 1.16e-17 75.1
Msa1216430 AT4G13850.4 41.250 80 47 0 70 149 34 113 3.13e-17 73.6
Msa1216430 AT4G13850.3 41.772 79 46 0 70 148 34 112 3.35e-17 73.9
Msa1216430 AT4G13850.2 41.772 79 46 0 70 148 34 112 4.12e-17 73.9
Msa1216430 AT4G13850.1 41.772 79 46 0 70 148 34 112 4.61e-17 73.9
Msa1216430 AT5G61030.2 32.331 133 74 3 17 149 2 118 6.81e-16 73.6
Msa1216430 AT5G61030.1 32.331 133 74 3 17 149 2 118 6.81e-16 73.6
Msa1216430 AT1G74230.1 40.206 97 55 2 51 147 16 109 2.40e-15 72.0
Msa1216430 AT4G39260.3 37.662 77 48 0 72 148 7 83 1.32e-14 65.9
Msa1216430 AT3G08000.1 39.252 107 56 1 41 147 20 117 1.97e-14 67.0
Msa1216430 AT3G20930.1 45.455 77 42 0 72 148 283 359 3.99e-14 68.9
Msa1216430 AT4G39260.2 37.662 77 48 0 72 148 7 83 4.14e-14 65.5
Msa1216430 AT5G06210.1 33.333 102 68 0 47 148 10 111 4.45e-14 65.9
Msa1216430 AT4G39260.1 37.662 77 48 0 72 148 7 83 1.02e-13 65.5
Msa1216430 AT5G47320.1 41.772 79 46 0 70 148 30 108 6.39e-13 64.3
Msa1216430 AT1G73530.1 34.783 92 60 0 71 162 77 168 8.82e-13 63.2
Msa1216430 AT3G46020.1 35.443 79 51 0 70 148 6 84 1.02e-12 61.2
Msa1216430 AT2G37510.1 33.333 81 54 0 64 144 27 107 1.11e-12 62.4
Msa1216430 AT3G20930.2 42.683 82 42 1 72 148 283 364 5.71e-12 62.8
Msa1216430 AT5G08695.1 37.179 78 49 0 72 149 228 305 7.54e-12 62.8
Msa1216430 AT2G27330.1 35.443 79 51 0 70 148 20 98 7.73e-12 59.3
Msa1216430 AT4G19610.1 36.364 77 49 0 72 148 295 371 7.73e-12 62.8
Msa1216430 AT3G10400.1 34.066 91 58 1 73 161 59 149 1.79e-11 60.8
Msa1216430 AT5G09880.1 35.165 91 58 1 56 145 250 340 4.10e-11 60.5
Msa1216430 AT3G47120.2 35.227 88 57 0 58 145 23 110 4.16e-11 60.1
Msa1216430 AT5G54580.1 31.776 107 62 2 54 149 28 134 4.51e-11 58.2
Msa1216430 AT3G47120.1 35.227 88 57 0 58 145 23 110 8.69e-11 59.3

Find 39 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACCTTTGATGAAGATTT+TGG 0.183699 8_1:-78186204 None:intergenic
CTCTAGGATTTGCATATATT+TGG 0.190867 8_1:+78187294 Msa1216430:CDS
GATGAAGGAATACTGCAATT+TGG 0.248379 8_1:-78186031 None:intergenic
ATGAAGGAATACTGCAATTT+GGG 0.266658 8_1:-78186030 None:intergenic
AATTTCTAGCATTGTGCCTT+GGG 0.295341 8_1:+78187605 Msa1216430:three_prime_UTR
TTATCAGTTGACATAGTGAT+AGG 0.318156 8_1:+78185953 Msa1216430:five_prime_UTR
CTATATTGAACAGTGTATTA+AGG 0.340770 8_1:+78187772 Msa1216430:three_prime_UTR
GCAGTATTCCTTCATCTGTC+TGG 0.342831 8_1:+78186038 Msa1216430:CDS
TCATGTCAGTTAAAATTTGT+AGG 0.382544 8_1:-78187520 None:intergenic
AATCTAATGGCACTTGTTGT+TGG 0.399086 8_1:+78185987 Msa1216430:exon
GAATTTCTAGCATTGTGCCT+TGG 0.403850 8_1:+78187604 Msa1216430:three_prime_UTR
TATGTTACAATTGCAAAGCC+TGG 0.436181 8_1:+78187471 Msa1216430:CDS
CAGTATTCCTTCATCTGTCT+GGG 0.458920 8_1:+78186039 Msa1216430:CDS
GGGCTGTGTTCAAAATCTAA+TGG 0.465130 8_1:+78185974 Msa1216430:exon
CACCTGTCACCGTCAGTGAT+TGG 0.480273 8_1:-78187711 None:intergenic
GACAGAAGATTCAGAGGAGG+TGG 0.482508 8_1:-78186159 None:intergenic
TCAAACTCCCAGACAGATGA+AGG 0.490829 8_1:-78186046 None:intergenic
TGCCAATCACTGACGGTGAC+AGG 0.491174 8_1:+78187709 Msa1216430:three_prime_UTR
TCAGCTGCAAAAGAGATGAA+TGG 0.519432 8_1:+78187341 Msa1216430:CDS
TCAACCAAAATCTTCATCAA+AGG 0.523578 8_1:+78186200 Msa1216430:CDS
GAAATTGAAAGTGAAAGAGT+TGG 0.531902 8_1:-78186098 None:intergenic
TATCAGTTGACATAGTGATA+GGG 0.532998 8_1:+78185954 Msa1216430:five_prime_UTR
ATTCAGAGGAGGTGGAGCAA+AGG 0.539853 8_1:-78186151 None:intergenic
ATTTCTAGCATTGTGCCTTG+GGG 0.561081 8_1:+78187606 Msa1216430:three_prime_UTR
TGCATATATTTGGTTTGTCG+AGG 0.565736 8_1:+78187304 Msa1216430:CDS
GAGGAGGTGGAGCAAAGGCA+TGG 0.566551 8_1:-78186146 None:intergenic
TTTCTAGCATTGTGCCTTGG+GGG 0.582549 8_1:+78187607 Msa1216430:three_prime_UTR
CTGTTCGGTGAAGTTACTCA+AGG 0.583315 8_1:+78187102 Msa1216430:CDS
TTTCGTTAAATGCACTTCAG+AGG 0.589643 8_1:-78187070 None:intergenic
GTGGAGCAAAGGCATGGAGA+AGG 0.596227 8_1:-78186140 None:intergenic
TGAAGGAATACTGCAATTTG+GGG 0.609882 8_1:-78186029 None:intergenic
CTAAAAGTGCCAATCACTGA+CGG 0.620131 8_1:+78187702 Msa1216430:three_prime_UTR
TGCACTTCAGAGGTTGAGAG+AGG 0.626279 8_1:-78187060 None:intergenic
ACATTCGACAGAAGATTCAG+AGG 0.629820 8_1:-78186165 None:intergenic
TGCAAAAGAGATGAATGGAA+AGG 0.634043 8_1:+78187346 Msa1216430:CDS
TGGAGAAGGTGCATAGCGAG+TGG 0.634968 8_1:-78186126 None:intergenic
AGAAGGTGCATAGCGAGTGG+AGG 0.655325 8_1:-78186123 None:intergenic
GTATTGTATGCTTACTAGAG+TGG 0.660770 8_1:-78187559 None:intergenic
TTCGACAGAAGATTCAGAGG+AGG 0.703143 8_1:-78186162 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGTAATTATAAATATATTT+TGG + chr8_1:78187122-78187141 Msa1216430:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_1:78187123-78187142 Msa1216430:intron 10.0%
!!! AGGTAATTATAAATATATTT+TGG + chr8_1:78187122-78187141 Msa1216430:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_1:78187123-78187142 Msa1216430:intron 10.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_1:78186426-78186445 Msa1216430:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_1:78186427-78186446 Msa1216430:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_1:78186513-78186532 Msa1216430:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_1:78186844-78186863 Msa1216430:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_1:78187235-78187254 Msa1216430:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_1:78187267-78187286 Msa1216430:CDS 15.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_1:78186426-78186445 Msa1216430:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_1:78186427-78186446 Msa1216430:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_1:78186513-78186532 Msa1216430:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_1:78186844-78186863 Msa1216430:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_1:78187235-78187254 Msa1216430:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_1:78187267-78187286 Msa1216430:CDS 15.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_1:78186837-78186856 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_1:78187171-78187190 Msa1216430:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_1:78187266-78187285 Msa1216430:CDS 20.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_1:78186837-78186856 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_1:78187171-78187190 Msa1216430:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_1:78187266-78187285 Msa1216430:CDS 20.0%
!!! CATCAAAGGTAACTTTATTT+TGG + chr8_1:78186214-78186233 Msa1216430:intron 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_1:78186551-78186570 Msa1216430:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_1:78186583-78186602 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_1:78186675-78186694 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_1:78186676-78186695 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_1:78186777-78186796 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_1:78186989-78187008 Msa1216430:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_1:78187186-78187205 Msa1216430:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_1:78187523-78187542 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_1:78187772-78187791 Msa1216430:three_prime_UTR 25.0%
!!! CATCAAAGGTAACTTTATTT+TGG + chr8_1:78186214-78186233 Msa1216430:intron 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_1:78186551-78186570 Msa1216430:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_1:78186583-78186602 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_1:78186675-78186694 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_1:78186676-78186695 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_1:78186777-78186796 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_1:78186989-78187008 Msa1216430:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_1:78187186-78187205 Msa1216430:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_1:78187523-78187542 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_1:78187772-78187791 Msa1216430:three_prime_UTR 25.0%
TTATCAGTTGACATAGTGAT+AGG + chr8_1:78185953-78185972 Msa1216430:five_prime_UTR 30.0%
TATCAGTTGACATAGTGATA+GGG + chr8_1:78185954-78185973 Msa1216430:five_prime_UTR 30.0%
ATGAAGGAATACTGCAATTT+GGG - chr8_1:78186033-78186052 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_1:78186101-78186120 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_1:78186197-78186216 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_1:78186200-78186219 Msa1216430:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_1:78186207-78186226 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_1:78186241-78186260 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_1:78186239-78186258 Msa1216430:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_1:78186240-78186259 Msa1216430:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_1:78186247-78186266 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_1:78186248-78186267 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_1:78186375-78186394 Msa1216430:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_1:78186447-78186466 Msa1216430:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_1:78186486-78186505 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_1:78186548-78186567 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_1:78186552-78186571 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_1:78186674-78186693 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_1:78186741-78186760 Msa1216430:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_1:78186784-78186803 Msa1216430:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_1:78186785-78186804 Msa1216430:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_1:78186788-78186807 Msa1216430:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_1:78186828-78186847 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_1:78186829-78186848 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG - chr8_1:78186902-78186921 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_1:78186916-78186935 Msa1216430:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG + chr8_1:78187137-78187156 Msa1216430:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_1:78187205-78187224 Msa1216430:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_1:78187294-78187313 Msa1216430:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_1:78187405-78187424 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_1:78187438-78187457 Msa1216430:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_1:78187492-78187511 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_1:78187696-78187715 None:intergenic 30.0%
TTATCAGTTGACATAGTGAT+AGG + chr8_1:78185953-78185972 Msa1216430:five_prime_UTR 30.0%
TATCAGTTGACATAGTGATA+GGG + chr8_1:78185954-78185973 Msa1216430:five_prime_UTR 30.0%
ATGAAGGAATACTGCAATTT+GGG - chr8_1:78186033-78186052 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_1:78186101-78186120 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_1:78186197-78186216 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_1:78186200-78186219 Msa1216430:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_1:78186207-78186226 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_1:78186241-78186260 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_1:78186239-78186258 Msa1216430:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_1:78186240-78186259 Msa1216430:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_1:78186247-78186266 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_1:78186248-78186267 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_1:78186375-78186394 Msa1216430:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_1:78186447-78186466 Msa1216430:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_1:78186486-78186505 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_1:78186548-78186567 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_1:78186552-78186571 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_1:78186674-78186693 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_1:78186741-78186760 Msa1216430:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_1:78186784-78186803 Msa1216430:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_1:78186785-78186804 Msa1216430:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_1:78186788-78186807 Msa1216430:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_1:78186828-78186847 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_1:78186829-78186848 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG - chr8_1:78186902-78186921 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_1:78186916-78186935 Msa1216430:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG + chr8_1:78187137-78187156 Msa1216430:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_1:78187205-78187224 Msa1216430:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_1:78187294-78187313 Msa1216430:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_1:78187405-78187424 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_1:78187438-78187457 Msa1216430:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_1:78187492-78187511 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_1:78187696-78187715 None:intergenic 30.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_1:78185987-78186006 Msa1216430:exon 35.0%
TGAAGGAATACTGCAATTTG+GGG - chr8_1:78186032-78186051 None:intergenic 35.0%
GATGAAGGAATACTGCAATT+TGG - chr8_1:78186034-78186053 None:intergenic 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_1:78186242-78186261 None:intergenic 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_1:78186384-78186403 Msa1216430:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_1:78186543-78186562 Msa1216430:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_1:78186974-78186993 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_1:78187019-78187038 Msa1216430:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG - chr8_1:78187073-78187092 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+CGG + chr8_1:78187087-78187106 Msa1216430:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_1:78187304-78187323 Msa1216430:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_1:78187346-78187365 Msa1216430:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_1:78187471-78187490 Msa1216430:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_1:78187562-78187581 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_1:78187605-78187624 Msa1216430:three_prime_UTR 35.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_1:78185987-78186006 Msa1216430:exon 35.0%
TGAAGGAATACTGCAATTTG+GGG - chr8_1:78186032-78186051 None:intergenic 35.0%
GATGAAGGAATACTGCAATT+TGG - chr8_1:78186034-78186053 None:intergenic 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_1:78186242-78186261 None:intergenic 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_1:78186384-78186403 Msa1216430:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_1:78186543-78186562 Msa1216430:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_1:78186974-78186993 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_1:78187019-78187038 Msa1216430:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG - chr8_1:78187073-78187092 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+CGG + chr8_1:78187087-78187106 Msa1216430:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_1:78187304-78187323 Msa1216430:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_1:78187346-78187365 Msa1216430:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_1:78187471-78187490 Msa1216430:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_1:78187562-78187581 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_1:78187605-78187624 Msa1216430:three_prime_UTR 35.0%
GGGCTGTGTTCAAAATCTAA+TGG + chr8_1:78185974-78185993 Msa1216430:exon 40.0%
CAGTATTCCTTCATCTGTCT+GGG + chr8_1:78186039-78186058 Msa1216430:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_1:78186168-78186187 None:intergenic 40.0%
TACTTGTTTCACTGTCTGCA+TGG + chr8_1:78186299-78186318 Msa1216430:intron 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_1:78186670-78186689 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_1:78186698-78186717 Msa1216430:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_1:78186871-78186890 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_1:78186872-78186891 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_1:78187001-78187020 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_1:78187007-78187026 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_1:78187023-78187042 Msa1216430:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_1:78187278-78187297 Msa1216430:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_1:78187341-78187360 Msa1216430:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_1:78187413-78187432 Msa1216430:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_1:78187442-78187461 Msa1216430:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_1:78187604-78187623 Msa1216430:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_1:78187606-78187625 Msa1216430:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_1:78187702-78187721 Msa1216430:three_prime_UTR 40.0%
AATGTGCAAGTGCAACATTG+GGG - chr8_1:78185928-78185947 None:intergenic 40.0%
GAATGTGCAAGTGCAACATT+GGG - chr8_1:78185929-78185948 None:intergenic 40.0%
AGAATGTGCAAGTGCAACAT+TGG - chr8_1:78185930-78185949 None:intergenic 40.0%
GGGCTGTGTTCAAAATCTAA+TGG + chr8_1:78185974-78185993 Msa1216430:exon 40.0%
CAGTATTCCTTCATCTGTCT+GGG + chr8_1:78186039-78186058 Msa1216430:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_1:78186168-78186187 None:intergenic 40.0%
TACTTGTTTCACTGTCTGCA+TGG + chr8_1:78186299-78186318 Msa1216430:intron 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_1:78186670-78186689 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_1:78186698-78186717 Msa1216430:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_1:78186871-78186890 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_1:78186872-78186891 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_1:78187001-78187020 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_1:78187007-78187026 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_1:78187023-78187042 Msa1216430:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_1:78187278-78187297 Msa1216430:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_1:78187341-78187360 Msa1216430:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_1:78187413-78187432 Msa1216430:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_1:78187442-78187461 Msa1216430:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_1:78187604-78187623 Msa1216430:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_1:78187606-78187625 Msa1216430:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_1:78187702-78187721 Msa1216430:three_prime_UTR 40.0%
GCAGTATTCCTTCATCTGTC+TGG + chr8_1:78186038-78186057 Msa1216430:CDS 45.0%
TCAAACTCCCAGACAGATGA+AGG - chr8_1:78186049-78186068 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_1:78186165-78186184 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_1:78186638-78186657 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_1:78186639-78186658 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_1:78186760-78186779 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_1:78186880-78186899 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG - chr8_1:78186964-78186983 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_1:78186970-78186989 None:intergenic 45.0%
! CTGTTCGGTGAAGTTACTCA+AGG + chr8_1:78187102-78187121 Msa1216430:CDS 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_1:78187422-78187441 Msa1216430:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_1:78187607-78187626 Msa1216430:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_1:78187624-78187643 None:intergenic 45.0%
GCAGTATTCCTTCATCTGTC+TGG + chr8_1:78186038-78186057 Msa1216430:CDS 45.0%
TCAAACTCCCAGACAGATGA+AGG - chr8_1:78186049-78186068 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_1:78186165-78186184 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_1:78186638-78186657 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_1:78186639-78186658 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_1:78186760-78186779 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_1:78186880-78186899 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG - chr8_1:78186964-78186983 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_1:78186970-78186989 None:intergenic 45.0%
! CTGTTCGGTGAAGTTACTCA+AGG + chr8_1:78187102-78187121 Msa1216430:CDS 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_1:78187422-78187441 Msa1216430:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_1:78187607-78187626 Msa1216430:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_1:78187624-78187643 None:intergenic 45.0%
ATTCAGAGGAGGTGGAGCAA+AGG - chr8_1:78186154-78186173 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_1:78186162-78186181 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_1:78186637-78186656 Msa1216430:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_1:78186720-78186739 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG - chr8_1:78187063-78187082 None:intergenic 50.0%
ATTCAGAGGAGGTGGAGCAA+AGG - chr8_1:78186154-78186173 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_1:78186162-78186181 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_1:78186637-78186656 Msa1216430:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_1:78186720-78186739 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG - chr8_1:78187063-78187082 None:intergenic 50.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_1:78186126-78186145 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_1:78186129-78186148 None:intergenic 55.0%
! GTGGAGCAAAGGCATGGAGA+AGG - chr8_1:78186143-78186162 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_1:78186469-78186488 Msa1216430:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_1:78186633-78186652 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_1:78186634-78186653 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_1:78187033-78187052 Msa1216430:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_1:78187034-78187053 Msa1216430:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_1:78187049-78187068 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_1:78187617-78187636 Msa1216430:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_1:78187709-78187728 Msa1216430:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_1:78187714-78187733 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_1:78186126-78186145 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_1:78186129-78186148 None:intergenic 55.0%
! GTGGAGCAAAGGCATGGAGA+AGG - chr8_1:78186143-78186162 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_1:78186469-78186488 Msa1216430:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_1:78186633-78186652 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_1:78186634-78186653 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_1:78187033-78187052 Msa1216430:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_1:78187034-78187053 Msa1216430:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_1:78187049-78187068 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_1:78187617-78187636 Msa1216430:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_1:78187709-78187728 Msa1216430:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_1:78187714-78187733 None:intergenic 55.0%
GAGGAGGTGGAGCAAAGGCA+TGG - chr8_1:78186149-78186168 None:intergenic 60.0%
AATGCATGCGGGCAGGGGTT+AGG - chr8_1:78186627-78186646 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_1:78186632-78186651 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_1:78186712-78186731 Msa1216430:intron 60.0%
GAGGAGGTGGAGCAAAGGCA+TGG - chr8_1:78186149-78186168 None:intergenic 60.0%
AATGCATGCGGGCAGGGGTT+AGG - chr8_1:78186627-78186646 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_1:78186632-78186651 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_1:78186712-78186731 Msa1216430:intron 60.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_1:78186629-78186648 Msa1216430:intron 65.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_1:78186629-78186648 Msa1216430:intron 65.0%
GCATGCGGGCAGGGGTTAGG+AGG - chr8_1:78186624-78186643 None:intergenic 70.0%
GCATGCGGGCAGGGGTTAGG+AGG - chr8_1:78186624-78186643 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 78185928 78187801 78185928 ID=Msa1216430;Name=Msa1216430
chr8_1 mRNA 78185928 78187801 78185928 ID=Msa1216430-mRNA-1;Parent=Msa1216430;Name=Msa1216430-mRNA-1;_AED=0.03;_eAED=0.03;_QI=65|1|1|1|1|1|4|268|162
chr8_1 exon 78185928 78186221 78185928 ID=Msa1216430-mRNA-1:exon:27002;Parent=Msa1216430-mRNA-1
chr8_1 exon 78187055 78187123 78187055 ID=Msa1216430-mRNA-1:exon:27003;Parent=Msa1216430-mRNA-1
chr8_1 exon 78187261 78187367 78187261 ID=Msa1216430-mRNA-1:exon:27004;Parent=Msa1216430-mRNA-1
chr8_1 exon 78187450 78187801 78187450 ID=Msa1216430-mRNA-1:exon:27005;Parent=Msa1216430-mRNA-1
chr8_1 five_prime_UTR 78185928 78185992 78185928 ID=Msa1216430-mRNA-1:five_prime_utr;Parent=Msa1216430-mRNA-1
chr8_1 CDS 78185993 78186221 78185993 ID=Msa1216430-mRNA-1:cds;Parent=Msa1216430-mRNA-1
chr8_1 CDS 78187055 78187123 78187055 ID=Msa1216430-mRNA-1:cds;Parent=Msa1216430-mRNA-1
chr8_1 CDS 78187261 78187367 78187261 ID=Msa1216430-mRNA-1:cds;Parent=Msa1216430-mRNA-1
chr8_1 CDS 78187450 78187533 78187450 ID=Msa1216430-mRNA-1:cds;Parent=Msa1216430-mRNA-1
chr8_1 three_prime_UTR 78187534 78187801 78187534 ID=Msa1216430-mRNA-1:three_prime_utr;Parent=Msa1216430-mRNA-1
Gene Sequence

>Msa1216430

ATGGCACTTGTTGTTGGTAGTAGTACTATATGCAAACCCCAAATTGCAGTATTCCTTCATCTGTCTGGGAGTTTGAAGAAACAAACAGTTTCGAAAATCACATTTCCAACTCTTTCACTTTCAATTTCTACCTCCACTCGCTATGCACCTTCTCCATGCCTTTGCTCCACCTCCTCTGAATCTTCTGTCGAATGTAATACGCCTTCTTCAACCAAAATCTTCATCAAAGGGCTTCCTCTCTCAACCTCTGAAGTGCATTTAACGAAAGTGTTTTCACTGTTCGGTGAAGTTACTCAAGTCAAGCTTTTGATAGATAAAAAAACTGGGCAGTCTCTAGGATTTGCATATATTTGGTTTGTCGAGGAAGAGTCTGCACAATCAGCTGCAAAAGAGATGAATGGAAAGTTTTTTTATGGCAGGTTTATTTATGTTACAATTGCAAAGCCTGGATCATCAAAAAGTTTGAAGAGAAAAGCCTACAAATTTTAA
Protein sequence

>Msa1216430

MALVVGSSTICKPQIAVFLHLSGSLKKQTVSKITFPTLSLSISTSTRYAPSPCLCSTSSESSVECNTPSSTKIFIKGLPLSTSEVHLTKVFSLFGEVTQVKLLIDKKTGQSLGFAYIWFVEEESAQSAAKEMNGKFFYGRFIYVTIAKPGSSKSLKRKAYKF*