AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1216180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216180 MtrunA17_Chr4g0065741 90.184 163 13 3 1 161 1 162 5.36e-93 266
Msa1216180 MtrunA17_Chr3g0088511 39.130 92 56 0 69 160 31 122 7.05e-19 77.8
Msa1216180 MtrunA17_Chr1g0198431 44.565 92 50 1 56 147 179 269 2.54e-16 73.9
Msa1216180 MtrunA17_Chr4g0031091 33.962 106 61 1 44 149 16 112 5.12e-16 69.7
Msa1216180 MtrunA17_Chr6g0481071 38.272 81 50 0 70 150 95 175 9.74e-16 70.9
Msa1216180 MtrunA17_Chr4g0061451 39.286 84 50 1 64 147 37 119 1.99e-15 71.2
Msa1216180 MtrunA17_Chr4g0035031 41.558 77 45 0 71 147 9 85 2.10e-15 70.1
Msa1216180 MtrunA17_Chr7g0263001 38.462 104 61 2 46 148 12 113 5.69e-15 67.8
Msa1216180 MtrunA17_Chr7g0266751 34.524 84 55 0 71 154 33 116 6.39e-15 67.8
Msa1216180 MtrunA17_Chr3g0119311 38.961 77 47 0 70 146 36 112 6.42e-15 67.4
Msa1216180 MtrunA17_Chr7g0219021 40.698 86 47 1 68 149 38 123 6.80e-15 69.7
Msa1216180 MtrunA17_Chr3g0121631 38.272 81 49 1 68 148 34 113 7.24e-14 65.1
Msa1216180 MtrunA17_Chr4g0035051 37.662 77 48 0 71 147 9 85 1.13e-13 63.9
Msa1216180 MtrunA17_Chr7g0259641 40.230 87 52 0 71 157 274 360 2.24e-13 66.6
Msa1216180 MtrunA17_Chr4g0035001 37.662 77 48 0 71 147 7 83 4.13e-13 63.5
Msa1216180 MtrunA17_Chr4g0050781 37.179 78 49 0 71 148 8 85 1.21e-12 63.2
Msa1216180 MtrunA17_Chr7g0259671 40.230 87 52 0 71 157 280 366 1.24e-12 64.7
Msa1216180 MtrunA17_Chr3g0086861 34.146 82 53 1 66 147 41 121 1.36e-12 64.3
Msa1216180 MtrunA17_Chr2g0327921 36.585 82 52 0 70 151 7 88 3.16e-12 59.3
Msa1216180 MtrunA17_Chr4g0042151 35.065 77 50 0 72 148 46 122 6.51e-12 60.8
Msa1216180 MtrunA17_Chr2g0278661 37.179 78 49 0 71 148 8 85 6.70e-12 61.6
Msa1216180 MtrunA17_Chr5g0430471 36.782 87 55 0 61 147 30 116 1.03e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1216180 AT5G19030.1 53.012 83 39 0 73 155 80 162 2.93e-25 95.5
Msa1216180 AT4G20030.1 43.529 85 48 0 65 149 35 119 2.36e-20 82.4
Msa1216180 AT5G19030.5 53.846 65 30 0 73 137 80 144 9.27e-19 78.2
Msa1216180 AT5G19030.2 54.839 62 28 0 73 134 80 141 1.08e-17 75.5
Msa1216180 AT3G23830.2 43.373 83 47 0 69 151 34 116 1.15e-17 75.1
Msa1216180 AT3G23830.1 43.373 83 47 0 69 151 34 116 1.15e-17 75.1
Msa1216180 AT4G13850.4 41.250 80 47 0 69 148 34 113 2.68e-17 73.9
Msa1216180 AT4G13850.3 41.772 79 46 0 69 147 34 112 2.90e-17 74.3
Msa1216180 AT4G13850.2 41.772 79 46 0 69 147 34 112 3.56e-17 74.3
Msa1216180 AT4G13850.1 41.772 79 46 0 69 147 34 112 3.99e-17 74.3
Msa1216180 AT5G61030.2 31.579 133 75 3 16 148 2 118 2.19e-15 72.0
Msa1216180 AT5G61030.1 31.579 133 75 3 16 148 2 118 2.19e-15 72.0
Msa1216180 AT1G74230.1 41.860 86 47 2 61 146 27 109 3.60e-15 71.2
Msa1216180 AT3G08000.1 40.187 107 55 2 40 146 20 117 1.47e-14 67.0
Msa1216180 AT4G39260.3 37.662 77 48 0 71 147 7 83 1.68e-14 65.5
Msa1216180 AT3G20930.1 45.455 77 42 0 71 147 283 359 3.92e-14 68.9
Msa1216180 AT4G39260.2 37.662 77 48 0 71 147 7 83 6.11e-14 65.1
Msa1216180 AT4G39260.1 37.662 77 48 0 71 147 7 83 1.50e-13 65.1
Msa1216180 AT5G47320.1 41.772 79 46 0 69 147 30 108 6.04e-13 64.3
Msa1216180 AT5G06210.1 36.709 79 50 0 69 147 33 111 7.97e-13 62.8
Msa1216180 AT1G73530.1 34.783 92 60 0 70 161 77 168 8.42e-13 63.2
Msa1216180 AT3G46020.1 35.443 79 51 0 69 147 6 84 1.36e-12 60.8
Msa1216180 AT2G37510.1 33.333 81 54 0 63 143 27 107 2.04e-12 61.6
Msa1216180 AT3G20930.2 42.683 82 42 1 71 147 283 364 5.25e-12 63.2
Msa1216180 AT2G27330.1 35.443 79 51 0 69 147 20 98 5.50e-12 59.7
Msa1216180 AT5G54580.1 32.710 107 61 2 53 148 28 134 1.37e-11 59.7
Msa1216180 AT5G08695.1 37.179 78 49 0 71 148 228 305 1.44e-11 62.0
Msa1216180 AT4G19610.1 36.364 77 49 0 71 147 295 371 1.49e-11 62.0
Msa1216180 AT3G10400.1 34.066 91 58 1 72 160 59 149 2.18e-11 60.5
Msa1216180 AT3G47120.2 35.227 88 57 0 57 144 23 110 9.42e-11 59.3

Find 38 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACCTTTGATGAAGATTT+TGG 0.183699 8_1:-77800199 None:intergenic
CTCTAGGATTTGCATATATT+TGG 0.190867 8_1:+77801289 Msa1216180:CDS
GATGAAGGAATACTGCAATT+TGG 0.248379 8_1:-77800026 None:intergenic
ATGAAGGAATACTGCAATTT+GGG 0.266658 8_1:-77800025 None:intergenic
AATTTCTAGCATTGTGCCTT+GGG 0.295341 8_1:+77801600 Msa1216180:three_prime_UTR
TTATCAGTTGACATAGTGAT+AGG 0.331423 8_1:+77799951 Msa1216180:five_prime_UTR
CTATATTGAACAGTGTATTA+AGG 0.340770 8_1:+77801767 Msa1216180:three_prime_UTR
GCAGTATTCCTTCATCTGTC+TGG 0.342831 8_1:+77800033 Msa1216180:CDS
GAGGAGGTGGAGCAAAAGTA+TGG 0.370330 8_1:-77800141 None:intergenic
TCATGTCAGTTAAAATTTGT+AGG 0.382544 8_1:-77801515 None:intergenic
AATCTAATGGCACTTGTTGT+TGG 0.399086 8_1:+77799985 Msa1216180:exon
GAATTTCTAGCATTGTGCCT+TGG 0.403850 8_1:+77801599 Msa1216180:three_prime_UTR
TATGTTACAATTGCAAAGCC+TGG 0.436181 8_1:+77801466 Msa1216180:CDS
CAGTATTCCTTCATCTGTCT+GGG 0.458920 8_1:+77800034 Msa1216180:CDS
CACCTGTCACCGTCAGTGAT+TGG 0.480273 8_1:-77801706 None:intergenic
GACAGAAGATTCAGAGGAGG+TGG 0.482508 8_1:-77800154 None:intergenic
GGGTTGTGTTCAAAATCTAA+TGG 0.482538 8_1:+77799972 Msa1216180:exon
TCAAACTCCCAGACAGATGA+AGG 0.490829 8_1:-77800041 None:intergenic
TGCCAATCACTGACGGTGAC+AGG 0.491174 8_1:+77801704 Msa1216180:three_prime_UTR
TCAGCTGCAAAAGAGATGAA+TGG 0.519432 8_1:+77801336 Msa1216180:CDS
TCAACCAAAATCTTCATCAA+AGG 0.523578 8_1:+77800195 Msa1216180:CDS
GAAATTGAAAGTGAAAGAGT+TGG 0.531902 8_1:-77800093 None:intergenic
TATCAGTTGACATAGTGATA+GGG 0.540213 8_1:+77799952 Msa1216180:five_prime_UTR
ATTTCTAGCATTGTGCCTTG+GGG 0.561081 8_1:+77801601 Msa1216180:three_prime_UTR
TGCATATATTTGGTTTGTCG+AGG 0.565736 8_1:+77801299 Msa1216180:CDS
TTTCTAGCATTGTGCCTTGG+GGG 0.582549 8_1:+77801602 Msa1216180:three_prime_UTR
CTGTTCGGTGAAGTTACTCA+AGG 0.583315 8_1:+77801097 Msa1216180:CDS
TTTCGTTAAATACACTTCAG+AGG 0.591937 8_1:-77801065 None:intergenic
GTGGAGCAAAAGTATGGAGA+AGG 0.601093 8_1:-77800135 None:intergenic
TGAAGGAATACTGCAATTTG+GGG 0.609882 8_1:-77800024 None:intergenic
TGGAGAAGGTGCATAGCGAG+TGG 0.615709 8_1:-77800121 None:intergenic
CTAAAAGTGCCAATCACTGA+CGG 0.620131 8_1:+77801697 Msa1216180:three_prime_UTR
TACACTTCAGAGGTTGAGAG+AGG 0.627741 8_1:-77801055 None:intergenic
ACATTCGACAGAAGATTCAG+AGG 0.629820 8_1:-77800160 None:intergenic
TGCAAAAGAGATGAATGGAA+AGG 0.634043 8_1:+77801341 Msa1216180:CDS
AGAAGGTGCATAGCGAGTGG+AGG 0.655325 8_1:-77800118 None:intergenic
GTATTGTATGCTTACTAGAG+TGG 0.660770 8_1:-77801554 None:intergenic
TTCGACAGAAGATTCAGAGG+AGG 0.703143 8_1:-77800157 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGTAATTATAAATATATTT+TGG + chr8_1:77801117-77801136 Msa1216180:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_1:77801118-77801137 Msa1216180:intron 10.0%
!!! AGGTAATTATAAATATATTT+TGG + chr8_1:77801117-77801136 Msa1216180:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_1:77801118-77801137 Msa1216180:intron 10.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_1:77800421-77800440 Msa1216180:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_1:77800422-77800441 Msa1216180:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_1:77800508-77800527 Msa1216180:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_1:77800839-77800858 Msa1216180:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_1:77801230-77801249 Msa1216180:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_1:77801262-77801281 Msa1216180:CDS 15.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_1:77800421-77800440 Msa1216180:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_1:77800422-77800441 Msa1216180:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_1:77800508-77800527 Msa1216180:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_1:77800839-77800858 Msa1216180:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_1:77801230-77801249 Msa1216180:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_1:77801262-77801281 Msa1216180:CDS 15.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_1:77800832-77800851 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_1:77801166-77801185 Msa1216180:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_1:77801261-77801280 Msa1216180:CDS 20.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_1:77800832-77800851 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_1:77801166-77801185 Msa1216180:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_1:77801261-77801280 Msa1216180:CDS 20.0%
!!! CATCAAAGGTAACTTTATTT+TGG + chr8_1:77800209-77800228 Msa1216180:intron 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_1:77800546-77800565 Msa1216180:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_1:77800578-77800597 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_1:77800670-77800689 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_1:77800671-77800690 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_1:77800772-77800791 None:intergenic 25.0%
!!! AATACAACTGTTTTTGTATG+TGG - chr8_1:77800897-77800916 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_1:77800984-77801003 Msa1216180:intron 25.0%
!!! TATTTTGGGTTTTTTCTGAA+AGG + chr8_1:77801132-77801151 Msa1216180:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_1:77801181-77801200 Msa1216180:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_1:77801518-77801537 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_1:77801767-77801786 Msa1216180:three_prime_UTR 25.0%
!!! CATCAAAGGTAACTTTATTT+TGG + chr8_1:77800209-77800228 Msa1216180:intron 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_1:77800546-77800565 Msa1216180:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_1:77800578-77800597 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_1:77800670-77800689 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_1:77800671-77800690 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_1:77800772-77800791 None:intergenic 25.0%
!!! AATACAACTGTTTTTGTATG+TGG - chr8_1:77800897-77800916 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_1:77800984-77801003 Msa1216180:intron 25.0%
!!! TATTTTGGGTTTTTTCTGAA+AGG + chr8_1:77801132-77801151 Msa1216180:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_1:77801181-77801200 Msa1216180:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_1:77801518-77801537 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_1:77801767-77801786 Msa1216180:three_prime_UTR 25.0%
TTATCAGTTGACATAGTGAT+AGG + chr8_1:77799951-77799970 Msa1216180:five_prime_UTR 30.0%
TATCAGTTGACATAGTGATA+GGG + chr8_1:77799952-77799971 Msa1216180:five_prime_UTR 30.0%
ATGAAGGAATACTGCAATTT+GGG - chr8_1:77800028-77800047 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_1:77800096-77800115 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_1:77800192-77800211 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_1:77800195-77800214 Msa1216180:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_1:77800202-77800221 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_1:77800236-77800255 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_1:77800234-77800253 Msa1216180:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_1:77800235-77800254 Msa1216180:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_1:77800242-77800261 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_1:77800243-77800262 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_1:77800370-77800389 Msa1216180:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_1:77800442-77800461 Msa1216180:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_1:77800481-77800500 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_1:77800543-77800562 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_1:77800547-77800566 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_1:77800669-77800688 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_1:77800736-77800755 Msa1216180:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_1:77800779-77800798 Msa1216180:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_1:77800780-77800799 Msa1216180:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_1:77800783-77800802 Msa1216180:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_1:77800823-77800842 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_1:77800824-77800843 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_1:77800911-77800930 Msa1216180:intron 30.0%
TTTCGTTAAATACACTTCAG+AGG - chr8_1:77801068-77801087 None:intergenic 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_1:77801200-77801219 Msa1216180:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_1:77801289-77801308 Msa1216180:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_1:77801400-77801419 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_1:77801433-77801452 Msa1216180:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_1:77801487-77801506 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_1:77801691-77801710 None:intergenic 30.0%
TTATCAGTTGACATAGTGAT+AGG + chr8_1:77799951-77799970 Msa1216180:five_prime_UTR 30.0%
TATCAGTTGACATAGTGATA+GGG + chr8_1:77799952-77799971 Msa1216180:five_prime_UTR 30.0%
ATGAAGGAATACTGCAATTT+GGG - chr8_1:77800028-77800047 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_1:77800096-77800115 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_1:77800192-77800211 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_1:77800195-77800214 Msa1216180:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_1:77800202-77800221 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_1:77800236-77800255 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_1:77800234-77800253 Msa1216180:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_1:77800235-77800254 Msa1216180:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_1:77800242-77800261 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_1:77800243-77800262 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_1:77800370-77800389 Msa1216180:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_1:77800442-77800461 Msa1216180:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_1:77800481-77800500 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_1:77800543-77800562 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_1:77800547-77800566 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_1:77800669-77800688 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_1:77800736-77800755 Msa1216180:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_1:77800779-77800798 Msa1216180:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_1:77800780-77800799 Msa1216180:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_1:77800783-77800802 Msa1216180:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_1:77800823-77800842 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_1:77800824-77800843 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_1:77800911-77800930 Msa1216180:intron 30.0%
TTTCGTTAAATACACTTCAG+AGG - chr8_1:77801068-77801087 None:intergenic 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_1:77801200-77801219 Msa1216180:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_1:77801289-77801308 Msa1216180:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_1:77801400-77801419 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_1:77801433-77801452 Msa1216180:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_1:77801487-77801506 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_1:77801691-77801710 None:intergenic 30.0%
GGGTTGTGTTCAAAATCTAA+TGG + chr8_1:77799972-77799991 Msa1216180:exon 35.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_1:77799985-77800004 Msa1216180:exon 35.0%
TGAAGGAATACTGCAATTTG+GGG - chr8_1:77800027-77800046 None:intergenic 35.0%
GATGAAGGAATACTGCAATT+TGG - chr8_1:77800029-77800048 None:intergenic 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_1:77800237-77800256 None:intergenic 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_1:77800379-77800398 Msa1216180:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_1:77800538-77800557 Msa1216180:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_1:77800969-77800988 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_1:77801014-77801033 Msa1216180:intron 35.0%
!!! ACGAAAGTGTTTTCACTGTT+CGG + chr8_1:77801082-77801101 Msa1216180:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_1:77801299-77801318 Msa1216180:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_1:77801341-77801360 Msa1216180:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_1:77801466-77801485 Msa1216180:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_1:77801557-77801576 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_1:77801600-77801619 Msa1216180:three_prime_UTR 35.0%
GGGTTGTGTTCAAAATCTAA+TGG + chr8_1:77799972-77799991 Msa1216180:exon 35.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_1:77799985-77800004 Msa1216180:exon 35.0%
TGAAGGAATACTGCAATTTG+GGG - chr8_1:77800027-77800046 None:intergenic 35.0%
GATGAAGGAATACTGCAATT+TGG - chr8_1:77800029-77800048 None:intergenic 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_1:77800237-77800256 None:intergenic 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_1:77800379-77800398 Msa1216180:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_1:77800538-77800557 Msa1216180:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_1:77800969-77800988 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_1:77801014-77801033 Msa1216180:intron 35.0%
!!! ACGAAAGTGTTTTCACTGTT+CGG + chr8_1:77801082-77801101 Msa1216180:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_1:77801299-77801318 Msa1216180:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_1:77801341-77801360 Msa1216180:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_1:77801466-77801485 Msa1216180:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_1:77801557-77801576 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_1:77801600-77801619 Msa1216180:three_prime_UTR 35.0%
CAGTATTCCTTCATCTGTCT+GGG + chr8_1:77800034-77800053 Msa1216180:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_1:77800163-77800182 None:intergenic 40.0%
TACTTGTTTCACTGTCTGCA+TGG + chr8_1:77800294-77800313 Msa1216180:intron 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_1:77800665-77800684 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_1:77800693-77800712 Msa1216180:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_1:77800866-77800885 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_1:77800867-77800886 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_1:77800996-77801015 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_1:77801002-77801021 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_1:77801018-77801037 Msa1216180:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_1:77801273-77801292 Msa1216180:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_1:77801336-77801355 Msa1216180:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_1:77801408-77801427 Msa1216180:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_1:77801437-77801456 Msa1216180:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_1:77801599-77801618 Msa1216180:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_1:77801601-77801620 Msa1216180:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_1:77801697-77801716 Msa1216180:three_prime_UTR 40.0%
CAGTATTCCTTCATCTGTCT+GGG + chr8_1:77800034-77800053 Msa1216180:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_1:77800163-77800182 None:intergenic 40.0%
TACTTGTTTCACTGTCTGCA+TGG + chr8_1:77800294-77800313 Msa1216180:intron 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_1:77800665-77800684 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_1:77800693-77800712 Msa1216180:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_1:77800866-77800885 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_1:77800867-77800886 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_1:77800996-77801015 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_1:77801002-77801021 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_1:77801018-77801037 Msa1216180:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_1:77801273-77801292 Msa1216180:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_1:77801336-77801355 Msa1216180:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_1:77801408-77801427 Msa1216180:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_1:77801437-77801456 Msa1216180:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_1:77801599-77801618 Msa1216180:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_1:77801601-77801620 Msa1216180:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_1:77801697-77801716 Msa1216180:three_prime_UTR 40.0%
GCAGTATTCCTTCATCTGTC+TGG + chr8_1:77800033-77800052 Msa1216180:CDS 45.0%
TCAAACTCCCAGACAGATGA+AGG - chr8_1:77800044-77800063 None:intergenic 45.0%
! GTGGAGCAAAAGTATGGAGA+AGG - chr8_1:77800138-77800157 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_1:77800160-77800179 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_1:77800633-77800652 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_1:77800634-77800653 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_1:77800755-77800774 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_1:77800875-77800894 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+TGG - chr8_1:77800959-77800978 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_1:77800965-77800984 None:intergenic 45.0%
TACACTTCAGAGGTTGAGAG+AGG - chr8_1:77801058-77801077 None:intergenic 45.0%
! CTGTTCGGTGAAGTTACTCA+AGG + chr8_1:77801097-77801116 Msa1216180:CDS 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_1:77801417-77801436 Msa1216180:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_1:77801602-77801621 Msa1216180:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_1:77801619-77801638 None:intergenic 45.0%
AATGTGCAAGTGCAGCATTG+GGG - chr8_1:77799926-77799945 None:intergenic 45.0%
GAATGTGCAAGTGCAGCATT+GGG - chr8_1:77799927-77799946 None:intergenic 45.0%
AGAATGTGCAAGTGCAGCAT+TGG - chr8_1:77799928-77799947 None:intergenic 45.0%
GCAGTATTCCTTCATCTGTC+TGG + chr8_1:77800033-77800052 Msa1216180:CDS 45.0%
TCAAACTCCCAGACAGATGA+AGG - chr8_1:77800044-77800063 None:intergenic 45.0%
! GTGGAGCAAAAGTATGGAGA+AGG - chr8_1:77800138-77800157 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_1:77800160-77800179 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_1:77800633-77800652 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_1:77800634-77800653 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_1:77800755-77800774 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_1:77800875-77800894 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+TGG - chr8_1:77800959-77800978 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_1:77800965-77800984 None:intergenic 45.0%
TACACTTCAGAGGTTGAGAG+AGG - chr8_1:77801058-77801077 None:intergenic 45.0%
! CTGTTCGGTGAAGTTACTCA+AGG + chr8_1:77801097-77801116 Msa1216180:CDS 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_1:77801417-77801436 Msa1216180:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_1:77801602-77801621 Msa1216180:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_1:77801619-77801638 None:intergenic 45.0%
GAGGAGGTGGAGCAAAAGTA+TGG - chr8_1:77800144-77800163 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_1:77800157-77800176 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_1:77800632-77800651 Msa1216180:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_1:77800715-77800734 None:intergenic 50.0%
GAGGAGGTGGAGCAAAAGTA+TGG - chr8_1:77800144-77800163 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_1:77800157-77800176 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_1:77800632-77800651 Msa1216180:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_1:77800715-77800734 None:intergenic 50.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_1:77800121-77800140 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_1:77800124-77800143 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_1:77800464-77800483 Msa1216180:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_1:77800628-77800647 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_1:77800629-77800648 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_1:77801028-77801047 Msa1216180:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_1:77801029-77801048 Msa1216180:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_1:77801044-77801063 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_1:77801612-77801631 Msa1216180:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_1:77801704-77801723 Msa1216180:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_1:77801709-77801728 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_1:77800121-77800140 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_1:77800124-77800143 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_1:77800464-77800483 Msa1216180:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_1:77800628-77800647 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_1:77800629-77800648 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_1:77801028-77801047 Msa1216180:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_1:77801029-77801048 Msa1216180:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_1:77801044-77801063 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_1:77801612-77801631 Msa1216180:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_1:77801704-77801723 Msa1216180:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_1:77801709-77801728 None:intergenic 55.0%
AATGCATGCGGGCAGGGGTT+AGG - chr8_1:77800622-77800641 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_1:77800627-77800646 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_1:77800707-77800726 Msa1216180:intron 60.0%
AATGCATGCGGGCAGGGGTT+AGG - chr8_1:77800622-77800641 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_1:77800627-77800646 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_1:77800707-77800726 Msa1216180:intron 60.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_1:77800624-77800643 Msa1216180:intron 65.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_1:77800624-77800643 Msa1216180:intron 65.0%
GCATGCGGGCAGGGGTTAGG+AGG - chr8_1:77800619-77800638 None:intergenic 70.0%
GCATGCGGGCAGGGGTTAGG+AGG - chr8_1:77800619-77800638 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 77799926 77801796 77799926 ID=Msa1216180;Name=Msa1216180
chr8_1 mRNA 77799926 77801796 77799926 ID=Msa1216180-mRNA-1;Parent=Msa1216180;Name=Msa1216180-mRNA-1;_AED=0.03;_eAED=0.03;_QI=65|1|1|1|1|1|4|268|161
chr8_1 exon 77799926 77800216 77799926 ID=Msa1216180-mRNA-1:exon:26879;Parent=Msa1216180-mRNA-1
chr8_1 exon 77801050 77801118 77801050 ID=Msa1216180-mRNA-1:exon:26880;Parent=Msa1216180-mRNA-1
chr8_1 exon 77801256 77801362 77801256 ID=Msa1216180-mRNA-1:exon:26881;Parent=Msa1216180-mRNA-1
chr8_1 exon 77801445 77801796 77801445 ID=Msa1216180-mRNA-1:exon:26882;Parent=Msa1216180-mRNA-1
chr8_1 five_prime_UTR 77799926 77799990 77799926 ID=Msa1216180-mRNA-1:five_prime_utr;Parent=Msa1216180-mRNA-1
chr8_1 CDS 77799991 77800216 77799991 ID=Msa1216180-mRNA-1:cds;Parent=Msa1216180-mRNA-1
chr8_1 CDS 77801050 77801118 77801050 ID=Msa1216180-mRNA-1:cds;Parent=Msa1216180-mRNA-1
chr8_1 CDS 77801256 77801362 77801256 ID=Msa1216180-mRNA-1:cds;Parent=Msa1216180-mRNA-1
chr8_1 CDS 77801445 77801528 77801445 ID=Msa1216180-mRNA-1:cds;Parent=Msa1216180-mRNA-1
chr8_1 three_prime_UTR 77801529 77801796 77801529 ID=Msa1216180-mRNA-1:three_prime_utr;Parent=Msa1216180-mRNA-1
Gene Sequence

>Msa1216180

ATGGCACTTGTTGTTGGTAGTAGTATATGCAAACCCCAAATTGCAGTATTCCTTCATCTGTCTGGGAGTTTGAAGAAACAAACAGTTTCGAAAATCACATTTCCAACTCTTTCACTTTCAATTTCTACCTCCACTCGCTATGCACCTTCTCCATACTTTTGCTCCACCTCCTCTGAATCTTCTGTCGAATGTAATACGCCTTCTTCAACCAAAATCTTCATCAAAGGGCTTCCTCTCTCAACCTCTGAAGTGTATTTAACGAAAGTGTTTTCACTGTTCGGTGAAGTTACTCAAGTCAAGCTTTTGATAGATAAAAAAACTGGGCAGTCTCTAGGATTTGCATATATTTGGTTTGTCGAGGAAGAGTCTGCACAATCAGCTGCAAAAGAGATGAATGGAAAGTTTTTTTATGGCAGGTTTATTTATGTTACAATTGCAAAGCCTGGATCATCAAAAAGTTTGAAGAGAAAAGCCTACAAATTTTAA
Protein sequence

>Msa1216180

MALVVGSSICKPQIAVFLHLSGSLKKQTVSKITFPTLSLSISTSTRYAPSPYFCSTSSESSVECNTPSSTKIFIKGLPLSTSEVYLTKVFSLFGEVTQVKLLIDKKTGQSLGFAYIWFVEEESAQSAAKEMNGKFFYGRFIYVTIAKPGSSKSLKRKAYKF*