Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217100 | RHN64206.1 | 29.603 | 277 | 137 | 9 | 445 | 684 | 97 | 352 | 3.71e-20 | 109 |
| Msa1217100 | RHN64206.1 | 92.203 | 808 | 63 | 0 | 1 | 808 | 123 | 930 | 0.0 | 1565 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217100 | sp|Q8RY23|NSH3_ARATH | 65.960 | 802 | 265 | 5 | 1 | 794 | 87 | 888 | 0.0 | 1115 |
| Msa1217100 | sp|Q8RY23|NSH3_ARATH | 30.556 | 324 | 164 | 11 | 430 | 712 | 46 | 349 | 1.24e-28 | 127 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1192120 | Msa1217100 | 0.806747 | 7.079431e-50 | -8.615850e-47 |
| Msa1216760 | Msa1217100 | 0.898517 | 4.909604e-77 | -8.615850e-47 |
| Msa1216960 | Msa1217100 | 0.909081 | 8.448432e-82 | -8.615850e-47 |
| Msa1216970 | Msa1217100 | 0.856329 | 3.463477e-62 | -8.615850e-47 |
| Msa1217080 | Msa1217100 | 0.942166 | 1.162952e-101 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217100 | MtrunA17_Chr4g0066571 | 92.203 | 808 | 63 | 0 | 1 | 808 | 123 | 930 | 0.0 | 1565 |
| Msa1217100 | MtrunA17_Chr4g0066571 | 29.603 | 277 | 137 | 9 | 445 | 684 | 97 | 352 | 3.41e-24 | 109 |
| Msa1217100 | MtrunA17_Chr4g0066541 | 90.494 | 810 | 76 | 1 | 1 | 810 | 85 | 893 | 0.0 | 1535 |
| Msa1217100 | MtrunA17_Chr4g0066541 | 28.532 | 361 | 191 | 12 | 418 | 734 | 32 | 369 | 2.74e-23 | 106 |
| Msa1217100 | MtrunA17_Chr2g0286751 | 78.294 | 797 | 167 | 3 | 1 | 796 | 86 | 877 | 0.0 | 1298 |
| Msa1217100 | MtrunA17_Chr2g0286751 | 31.757 | 296 | 144 | 10 | 445 | 703 | 60 | 334 | 7.79e-26 | 114 |
| Msa1217100 | MtrunA17_Chr2g0286771 | 72.180 | 266 | 73 | 1 | 532 | 796 | 33 | 298 | 3.94e-131 | 393 |
| Msa1217100 | MtrunA17_Chr2g0286761 | 85.000 | 80 | 12 | 0 | 18 | 97 | 22 | 101 | 1.02e-40 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1217100 | AT5G18860.1 | 65.960 | 802 | 265 | 5 | 1 | 794 | 87 | 888 | 0.0 | 1115 |
| Msa1217100 | AT5G18860.1 | 30.556 | 324 | 164 | 11 | 430 | 712 | 46 | 349 | 1.26e-29 | 127 |
| Msa1217100 | AT5G18860.2 | 65.960 | 802 | 265 | 5 | 1 | 794 | 87 | 888 | 0.0 | 1115 |
| Msa1217100 | AT5G18860.2 | 30.556 | 324 | 164 | 11 | 430 | 712 | 46 | 349 | 1.26e-29 | 127 |
| Msa1217100 | AT5G18890.1 | 62.101 | 533 | 197 | 4 | 269 | 799 | 14 | 543 | 0.0 | 687 |
| Msa1217100 | AT5G18890.1 | 29.200 | 250 | 120 | 8 | 1 | 230 | 216 | 428 | 9.74e-19 | 91.3 |
| Msa1217100 | AT5G18870.1 | 65.775 | 187 | 44 | 3 | 1 | 186 | 84 | 251 | 3.54e-80 | 258 |
| Msa1217100 | AT5G18870.1 | 36.325 | 234 | 107 | 9 | 429 | 642 | 42 | 253 | 1.32e-27 | 113 |
Find 204 sgRNAs with CRISPR-Local
Find 1996 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACATCTCCTAGACCAAC+TGG | 0.452684 | 8_1:+79311286 | None:intergenic |
| AAACATAAGCTCCAGAATTC+AGG | 0.350181 | 8_1:-79310725 | Msa1217100:intron |
| AAACCATATGGAATATCTGA+TGG | 0.570401 | 8_1:-79312426 | Msa1217100:CDS |
| AAACGTCCTCCAAGTCCTAC+GGG | 0.525988 | 8_1:+79313859 | None:intergenic |
| AAAGCCATTCCTCATGGGAG+TGG | 0.551840 | 8_1:-79311208 | Msa1217100:CDS |
| AAATGCATCAAAGAATTGTT+CGG | 0.431938 | 8_1:+79312813 | None:intergenic |
| AACCTTTGCCAAATTAGTCA+AGG | 0.520889 | 8_1:+79310759 | None:intergenic |
| AAGAATTATTTCAGGTACAC+CGG | 0.597223 | 8_1:-79310919 | Msa1217100:intron |
| AAGATATTGAATTCTGCATA+AGG | 0.415808 | 8_1:+79313422 | None:intergenic |
| AAGCCATTCCTCATGGGAGT+GGG | 0.497973 | 8_1:-79311207 | Msa1217100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCACACATTTGTGACAT+TGG | + | chr8_1:79309697-79309716 | None:intergenic | 30.0% | |
| ATGACTTACTTTACATGATG+GGG | - | chr8_1:79309841-79309860 | Msa1217100:intron | 30.0% | |
| ACTGTTACCTGATCTATAAT+TGG | + | chr8_1:79309951-79309970 | None:intergenic | 30.0% | |
| AAAAAAAGGCCTATGTAAGT+GGG | + | chr8_1:79310491-79310510 | None:intergenic | 30.0% | |
| TAAAAAAAGGCCTATGTAAG+TGG | + | chr8_1:79310492-79310511 | None:intergenic | 30.0% | |
| CTAATTTGATCATGGTATCA+GGG | - | chr8_1:79310990-79311009 | Msa1217100:intron | 30.0% | |
| AGATGTTGTATTCTGCATAA+GGG | + | chr8_1:79311298-79311317 | None:intergenic | 30.0% | |
| AAGATGTTGTATTCTGCATA+AGG | + | chr8_1:79311299-79311318 | None:intergenic | 30.0% | |
| TATGCAGAATACAACATCTT+TGG | - | chr8_1:79311299-79311318 | Msa1217100:CDS | 30.0% | |
| AATAAAGAATGTGTATGACC+TGG | + | chr8_1:79311343-79311362 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 79309495 | 79333779 | 79309495 | ID=Msa1217100;Name=Msa1217100 |
| chr8_1 | mRNA | 79309495 | 79333779 | 79309495 | ID=Msa1217100-mRNA-1;Parent=Msa1217100;Name=Msa1217100-mRNA-1;_AED=0.31;_eAED=0.31;_QI=238|1|0.92|1|0.83|0.76|13|0|816 |
| chr8_1 | exon | 79309495 | 79309845 | 79309495 | ID=Msa1217100-mRNA-1:exon:27602;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79310017 | 79310322 | 79310017 | ID=Msa1217100-mRNA-1:exon:27601;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79310726 | 79310927 | 79310726 | ID=Msa1217100-mRNA-1:exon:27600;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79311145 | 79311398 | 79311145 | ID=Msa1217100-mRNA-1:exon:27599;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79311666 | 79311863 | 79311666 | ID=Msa1217100-mRNA-1:exon:27598;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79311964 | 79312086 | 79311964 | ID=Msa1217100-mRNA-1:exon:27597;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79312293 | 79312577 | 79312293 | ID=Msa1217100-mRNA-1:exon:27596;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79312723 | 79312899 | 79312723 | ID=Msa1217100-mRNA-1:exon:27595;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79313397 | 79313732 | 79313397 | ID=Msa1217100-mRNA-1:exon:27594;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79313813 | 79313923 | 79313813 | ID=Msa1217100-mRNA-1:exon:27593;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79322721 | 79322900 | 79322721 | ID=Msa1217100-mRNA-1:exon:27592;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79325534 | 79325539 | 79325534 | ID=Msa1217100-mRNA-1:exon:27591;Parent=Msa1217100-mRNA-1 |
| chr8_1 | exon | 79333620 | 79333779 | 79333620 | ID=Msa1217100-mRNA-1:exon:27590;Parent=Msa1217100-mRNA-1 |
| chr8_1 | five_prime_UTR | 79333620 | 79333779 | 79333620 | ID=Msa1217100-mRNA-1:five_prime_utr;Parent=Msa1217100-mRNA-1 |
| chr8_1 | five_prime_UTR | 79325534 | 79325539 | 79325534 | ID=Msa1217100-mRNA-1:five_prime_utr;Parent=Msa1217100-mRNA-1 |
| chr8_1 | five_prime_UTR | 79322829 | 79322900 | 79322829 | ID=Msa1217100-mRNA-1:five_prime_utr;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79322721 | 79322828 | 79322721 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79313813 | 79313923 | 79313813 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79313397 | 79313732 | 79313397 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79312723 | 79312899 | 79312723 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79312293 | 79312577 | 79312293 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79311964 | 79312086 | 79311964 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79311666 | 79311863 | 79311666 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79311145 | 79311398 | 79311145 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79310726 | 79310927 | 79310726 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79310017 | 79310322 | 79310017 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| chr8_1 | CDS | 79309495 | 79309845 | 79309495 | ID=Msa1217100-mRNA-1:cds;Parent=Msa1217100-mRNA-1 |
| Gene Sequence |
>Msa1217100 ATGATGGGGAGGGATGATATTGCTGTTGGAATTGGTGGCGAGGGTGGAATACTGTCAAATGGTACCATACTCCCAAATGTTGGTGGATATCTACCAATAATAGAACAGGGAATGACAACAATAGGAGGTTGCAGATACAGGCAGGCCATTCCCGTAGGACTTGGAGGACGTTTAGATATTGATGCAAATTATGGTATCAGGAAAGCTTTCCTCCCACAGGGAAAAAGGAAATATACACCACTAGAGCAACCAACTGCTCAACAGGTGCTGATTGAAAAAGTGTCTGCAGGTCCCACAACTTTGTTTATGATGGGAGCTCATACAAATGTTGCAATTTTCCTAATGAATAATCCACACCTCAAGAAAAATGTGGTACATATTTATATAATGGGTGGAGGTGTAAGGTCAAGCAATCCAACTGGTTGTTGCCCTAAAAATGCTTCATCTACCTGTGTGCCTCGACAGTGTGGTGACCATGGAAATATGTTTACAGATTATAATACTAACCCTTATGCAGAATTCAATATCTTTGGAGACCCTTTTGCAGCTTACCAGGTGATTCATTCTGGAATTCCAGTTACCCTAGTTCCTCTTGATGCATCAAACACAATCCCCATCACCGAACAATTCTTTGATGCATTTGAAAAGAGTCAAGACACATATGAAGCACAATACTGTTTCAAATCCTTGAAAATATCTCGTGATACTTGGTTCGACAACGAATTCTATTCGAGTTTTTTTTTGTGGGACTCTTTTATGACTGGTGTGGCTATCTCAATCATGAGTAAACCCAATAATCACAAAGGAGATAATGAATTTGCAGAAATGGAGTATATGAACATAACTGTAATTACCTCAAATAAACCATATGGAATATCTGATGGCTCCAATCCATTATTTAATGGCCTAAAAGTTCCCAAGTTCAATCTTGAAAAAGGTGGTGTCCATAGTGGTCATATTCAACAAGGACTCAGAGATCCTCTTTGCTTCGTGGAGAATGGCAAAGGAAAATGTCAGGATGGTTACACAAAAGAGGTAGGTGGTCCAGGCTCAGTGAGAGTACTTGTTGCTACAAAAGCAAAGCCTAACCGAGATGTTGGGAGTTCACTTGACAGGGAATATTTCATAAGATTCTTAGATGTTCTAAAGCAACCACGACAAGCAGGGAGATACAACTTTACTACTCAGTTTCCCTACTACAAAGAAGTAACTTACAAGCCAAACATTCAAAATAAAAAATTGGGAAAACCTGTTGTTTTTGACATGGACATGAGTGCAGGAGATTTTCTAGCTCTATTTTACCTTCTTAAAGTGCCTGTTCAAGTAATCGACCTTAAGGCGATCATTGTGAGTCCTACTGGATGGGCAAATGCAGCAACAATAGACATAGTTTATGACATACTTCACATGATGGGTCGTGATGATATACCAGTTGGTCTAGGAGATGTTTTTGCAATGAATCAGGCAGATCCAATACTCCCAGTCGTTGGAGATTGCAAGTATGTTAAAGCCATTCCTCATGGGAGTGGGGGGTACCTCGATTCTGACACTCTTTATGGTCTGGCTCGACATCTACCTCGCAGCCCGAGAAGGTACACCGGAGAAAAATCGGTGAAGTTTGGGGCTCCACGGGATACAGATCACCCTGAACTTAGACAGCCACTAGCTATGGAAGTTTGGGAGTCTGTATTGCAAACAATGAAACCAGCGTCCAATATTACAGTATTAACTAACGGACCCTTGACTAATTTGGCAAAGGTTCTTTCAATGAAAAACATAAGCTCCAGAATTCAGGAGGTTTATGTAGTGGGGGGACACATCAGCAGGAATACAGAAGACAAAGGAAATGTTTTTTCAGTTCCTTCAAACCAATATGCAGAATTCAACATGTTCCTAGATCCTTTAGCAGCCAAAACAGTGTTCGAATCAGAAGTTAACATCACACTCATTCCACTGAGAATTCAGCGTAGAGTGAGTTCGTTTGCAACGGTTATAGGTAGGCTGAACAGGACAAGAAAAACACCAGAGGTTCTGTTTTCCAAGCGTCTATTGTCAAGCCTACACCGTTTGAAGCAAATTCATAACGGATATCACCATACGGACACATTCCTGGGAGAAATTCTAGGTGCAGTAGTCTTGGCTGAGAAGGCTTCAAGTCTGAAGCCAAAATTTGAGGTGAAGCCCATTAAAGTCCTAGCTAGTGGCATTGAATCAAGTGATGGAAAAATGGTGGTGGATGAGAAACATAGAAAGTTAGTCAGAATATTAAGCAATGTTGAGGAAAAAGCTTATTATAATTTGTATGTAAACAAGCTTGGTGATCAGTATCAGTCAGCTAAGGTAGGAAGCTTTAAAGAACAGACAAGAATGTGGAGACATCCTCGTGATGATAAGCCTAGTGAAGAAAAATGTGCTCAATTCCACGTCTATAAGGCGTTTTATCAAATGTGA |
| Protein sequence |
>Msa1217100 MMGRDDIAVGIGGEGGILSNGTILPNVGGYLPIIEQGMTTIGGCRYRQAIPVGLGGRLDIDANYGIRKAFLPQGKRKYTPLEQPTAQQVLIEKVSAGPTTLFMMGAHTNVAIFLMNNPHLKKNVVHIYIMGGGVRSSNPTGCCPKNASSTCVPRQCGDHGNMFTDYNTNPYAEFNIFGDPFAAYQVIHSGIPVTLVPLDASNTIPITEQFFDAFEKSQDTYEAQYCFKSLKISRDTWFDNEFYSSFFLWDSFMTGVAISIMSKPNNHKGDNEFAEMEYMNITVITSNKPYGISDGSNPLFNGLKVPKFNLEKGGVHSGHIQQGLRDPLCFVENGKGKCQDGYTKEVGGPGSVRVLVATKAKPNRDVGSSLDREYFIRFLDVLKQPRQAGRYNFTTQFPYYKEVTYKPNIQNKKLGKPVVFDMDMSAGDFLALFYLLKVPVQVIDLKAIIVSPTGWANAATIDIVYDILHMMGRDDIPVGLGDVFAMNQADPILPVVGDCKYVKAIPHGSGGYLDSDTLYGLARHLPRSPRRYTGEKSVKFGAPRDTDHPELRQPLAMEVWESVLQTMKPASNITVLTNGPLTNLAKVLSMKNISSRIQEVYVVGGHISRNTEDKGNVFSVPSNQYAEFNMFLDPLAAKTVFESEVNITLIPLRIQRRVSSFATVIGRLNRTRKTPEVLFSKRLLSSLHRLKQIHNGYHHTDTFLGEILGAVVLAEKASSLKPKFEVKPIKVLASGIESSDGKMVVDEKHRKLVRILSNVEEKAYYNLYVNKLGDQYQSAKVGSFKEQTRMWRHPRDDKPSEEKCAQFHVYKAFYQM* |
