AlfalfaGEDB | Msa1219420

M. sativa cultivar zhongmu-4 / Msa1219420

KEGG: K14440
GO: GO:0000018 GO:0000228 GO:0000733 GO:0003674 GO:0003676 GO:0003824 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0008156 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0017111 GO:0019219 GO:0019222 GO:0031297 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031593 GO:0031974 GO:0031981 GO:0032392 GO:0033554 GO:0034641 GO:0034645 GO:0036292 GO:0036310 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045005 GO:0045910 GO:0045934 GO:0046483 GO:0048478 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0051276 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070530 GO:0071103 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090305 GO:0090329 GO:0097159 GO:0097617 GO:0140030 GO:0140097 GO:1901360 GO:1901363 GO:1901576 GO:2000104 GO:2000112 GO:2000113
PFAM: PF00176.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219420	AES92088.2	97.126	696	9	3	1	692	1	689	0.0	1379

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219420	sp\|B2ZFP3\|SMAL1_DANRE	39.928	556	286	10	80	620	238	760	8.36e-114	364

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219420	G7JR21	97.126	696	9	3	1	692	1	689	0.0	1379

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TFs/TRs:

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Gene ID	Type	Classification
Msa1219420	TR	SNF2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1219420	Msa1386420	0.940468	2.216619e-100	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219420	MtrunA17_Chr4g0069451	96.889	675	10	3	1	671	1	668	0.0	1336
Msa1219420	MtrunA17_Chr3g0086111	36.996	273	145	6	168	426	178	437	1.56e-45	175
Msa1219420	MtrunA17_Chr3g0086111	40.559	143	85	0	487	629	548	690	4.59e-27	118
Msa1219420	MtrunA17_Chr5g0394201	26.296	540	330	19	157	658	342	851	8.69e-35	142
Msa1219420	MtrunA17_Chr4g0017211	26.812	552	293	15	168	629	1441	1971	1.07e-34	142
Msa1219420	MtrunA17_Chr3g0095091	25.887	479	296	15	176	614	570	1029	4.15e-33	137
Msa1219420	MtrunA17_Chr5g0442141	25.155	485	292	13	176	614	616	1075	6.73e-33	136
Msa1219420	MtrunA17_Chr4g0066741	23.718	468	317	11	170	614	181	631	2.01e-32	134
Msa1219420	MtrunA17_Chr3g0137091	25.572	481	299	13	177	614	289	753	2.91e-32	134
Msa1219420	MtrunA17_Chr2g0286291	24.204	471	311	14	170	614	184	634	1.64e-31	132

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1219420	AT1G48310.1	68.741	675	196	6	6	671	5	673	0.0	937
Msa1219420	AT1G48310.2	68.289	678	199	6	6	671	5	678	0.0	935
Msa1219420	AT1G48310.3	70.894	615	175	3	6	620	5	615	0.0	896
Msa1219420	AT1G48310.4	68.254	504	156	3	6	509	5	504	0.0	706
Msa1219420	AT5G07810.1	35.363	509	263	11	167	623	194	688	2.81e-81	281
Msa1219420	AT5G19310.2	27.291	491	304	16	158	614	102	573	4.93e-35	142
Msa1219420	AT5G19310.1	27.291	491	304	16	158	614	366	837	5.22e-35	143
Msa1219420	AT3G06010.1	25.885	537	337	17	157	658	382	892	9.10e-35	142
Msa1219420	AT2G13370.1	26.327	471	300	12	177	614	625	1081	2.78e-34	141
Msa1219420	AT2G13370.2	26.327	471	300	12	177	614	625	1081	2.78e-34	141

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Find 148 sgRNAs with CRISPR-Local

Find 711 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCAAAATTCATCTGGA+TGG	0.528270	8_1:+82255196	Msa1219420:CDS
AAAGATGCAAAGTACACTCA+TGG	0.554876	8_1:+82255252	Msa1219420:CDS
AAAGCAAACTGGAAAATTTG+GGG	0.668375	8_1:+82260416	Msa1219420:CDS
AACATTGGTAGTGTTGTTTG+TGG	0.445669	8_1:-82251357	None:intergenic
AACGCATGCAGCTACAGCAA+TGG	0.523209	8_1:-82254755	None:intergenic
AAGAAATTAGTTACTCTTTG+AGG	0.355154	8_1:-82251212	None:intergenic
AAGCAGTTGTTCTCTTTGCC+TGG	0.369977	8_1:-82256476	None:intergenic
AAGGTGTGCCTCCATCAATC+CGG	0.538088	8_1:-82259627	None:intergenic
AAGGTTCTTAAAGACATATC+TGG	0.399289	8_1:+82251664	Msa1219420:CDS
AAGTAGGTGGTCAGCACAGC+TGG	0.442258	8_1:+82261179	Msa1219420:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCCTTCAAAAAAATCAAG+CGG	+	chr8_1:82251046-82251065	Msa1219420:five_prime_UTR	30.0%
TGGCATTCCAAAAAGATTTA+GGG	-	chr8_1:82251597-82251616	None:intergenic	30.0%
TTGGCATTCCAAAAAGATTT+AGG	-	chr8_1:82251598-82251617	None:intergenic	30.0%
AAGGTTCTTAAAGACATATC+TGG	+	chr8_1:82251664-82251683	Msa1219420:CDS	30.0%
CATATCTGGTTATAATGTTC+AGG	+	chr8_1:82251678-82251697	Msa1219420:CDS	30.0%
CTTAAAGTGATAATCTAAGG+AGG	-	chr8_1:82251861-82251880	None:intergenic	30.0%
GGTCTTAAAGTGATAATCTA+AGG	-	chr8_1:82251864-82251883	None:intergenic	30.0%
ATTGTGAAGTAGCTTAATCA+TGG	+	chr8_1:82252462-82252481	Msa1219420:intron	30.0%
TTGTGAAGTAGCTTAATCAT+GGG	+	chr8_1:82252463-82252482	Msa1219420:intron	30.0%
ACAGTCTATTAGCAAAGAAA+AGG	-	chr8_1:82252658-82252677	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	82250987	82261281	82250987	ID=Msa1219420;Name=Msa1219420
chr8_1	mRNA	82250987	82261281	82250987	ID=Msa1219420-mRNA-1;Parent=Msa1219420;Name=Msa1219420-mRNA-1;_AED=0.17;_eAED=0.18;_QI=108\|0.95\|0.91\|1\|1\|1\|23\|0\|701
chr8_1	exon	82250987	82251220	82250987	ID=Msa1219420-mRNA-1:exon:29215;Parent=Msa1219420-mRNA-1
chr8_1	exon	82251302	82251466	82251302	ID=Msa1219420-mRNA-1:exon:29216;Parent=Msa1219420-mRNA-1
chr8_1	exon	82251568	82251699	82251568	ID=Msa1219420-mRNA-1:exon:29217;Parent=Msa1219420-mRNA-1
chr8_1	exon	82253091	82253157	82253091	ID=Msa1219420-mRNA-1:exon:29218;Parent=Msa1219420-mRNA-1
chr8_1	exon	82253285	82253351	82253285	ID=Msa1219420-mRNA-1:exon:29219;Parent=Msa1219420-mRNA-1
chr8_1	exon	82253473	82253539	82253473	ID=Msa1219420-mRNA-1:exon:29220;Parent=Msa1219420-mRNA-1
chr8_1	exon	82254754	82254837	82254754	ID=Msa1219420-mRNA-1:exon:29221;Parent=Msa1219420-mRNA-1
chr8_1	exon	82254921	82254962	82254921	ID=Msa1219420-mRNA-1:exon:29222;Parent=Msa1219420-mRNA-1
chr8_1	exon	82255133	82255288	82255133	ID=Msa1219420-mRNA-1:exon:29223;Parent=Msa1219420-mRNA-1
chr8_1	exon	82256438	82256515	82256438	ID=Msa1219420-mRNA-1:exon:29224;Parent=Msa1219420-mRNA-1
chr8_1	exon	82256928	82256996	82256928	ID=Msa1219420-mRNA-1:exon:29225;Parent=Msa1219420-mRNA-1
chr8_1	exon	82257092	82257157	82257092	ID=Msa1219420-mRNA-1:exon:29226;Parent=Msa1219420-mRNA-1
chr8_1	exon	82257260	82257388	82257260	ID=Msa1219420-mRNA-1:exon:29227;Parent=Msa1219420-mRNA-1
chr8_1	exon	82257479	82257535	82257479	ID=Msa1219420-mRNA-1:exon:29228;Parent=Msa1219420-mRNA-1
chr8_1	exon	82258205	82258294	82258205	ID=Msa1219420-mRNA-1:exon:29229;Parent=Msa1219420-mRNA-1
chr8_1	exon	82258498	82258563	82258498	ID=Msa1219420-mRNA-1:exon:29230;Parent=Msa1219420-mRNA-1
chr8_1	exon	82259462	82259530	82259462	ID=Msa1219420-mRNA-1:exon:29231;Parent=Msa1219420-mRNA-1
chr8_1	exon	82259607	82259711	82259607	ID=Msa1219420-mRNA-1:exon:29232;Parent=Msa1219420-mRNA-1
chr8_1	exon	82259856	82259981	82259856	ID=Msa1219420-mRNA-1:exon:29233;Parent=Msa1219420-mRNA-1
chr8_1	exon	82260248	82260306	82260248	ID=Msa1219420-mRNA-1:exon:29234;Parent=Msa1219420-mRNA-1
chr8_1	exon	82260405	82260441	82260405	ID=Msa1219420-mRNA-1:exon:29235;Parent=Msa1219420-mRNA-1
chr8_1	exon	82260573	82260724	82260573	ID=Msa1219420-mRNA-1:exon:29236;Parent=Msa1219420-mRNA-1
chr8_1	exon	82261185	82261281	82261185	ID=Msa1219420-mRNA-1:exon:29237;Parent=Msa1219420-mRNA-1
chr8_1	five_prime_UTR	82250987	82251094	82250987	ID=Msa1219420-mRNA-1:five_prime_utr;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82251095	82251220	82251095	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82251302	82251466	82251302	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82251568	82251699	82251568	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82253091	82253157	82253091	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82253285	82253351	82253285	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82253473	82253539	82253473	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82254754	82254837	82254754	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82254921	82254962	82254921	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82255133	82255288	82255133	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82256438	82256515	82256438	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82256928	82256996	82256928	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82257092	82257157	82257092	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82257260	82257388	82257260	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82257479	82257535	82257479	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82258205	82258294	82258205	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82258498	82258563	82258498	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82259462	82259530	82259462	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82259607	82259711	82259607	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82259856	82259981	82259856	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82260248	82260306	82260248	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82260405	82260441	82260405	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82260573	82260724	82260573	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1
chr8_1	CDS	82261185	82261281	82261185	ID=Msa1219420-mRNA-1:cds;Parent=Msa1219420-mRNA-1

Gene Sequence

>Msa1219420

ATGGAACCAGAAGACTGGGATTTGAGCGGTGAAGACCTCGATTCTCTTGAAAGAGACGCTTTCCAGAAAATCGCTCAACTCCGCTCCCACCCTTCAAACCCTCCTCCCCCTTCACGTCCTCAAAGAGTTGATGCTTTGCCCCAAGCAGCTGCTAGAGCACTTCCAACGTCGATAAAATCAGCCACAAACAACACTACCAATGTTGAGCATTCTAAAGAATTGACTAAAGTTTCGGTCAAATTTTTTCTCCATTCCACTGGAAACATTGCGGCAAAGTTTCAGTATGATCAGGTAATAGTTGCTGCTTTTAGAAGAATCCCTAAATCTTTTTGGAATGCCAAAGAACGCTTATGGTTGTTCCCTCTCTCCTCTTTGTCAGAAGCCGAAAAGGTTCTTAAAGACATATCTGGTTATAATGTTCAGGTGGAGAACTTGGATCCCTTAGTGCAACGTGCTATTACTGCTGCCACTTCTGTTCCTGATCTTCGAGATCGATATGATAAGATCCCTAGCGATGTTGAATCAAAACTTTTGCCATTTCAGCGAGACGGTATAAGGTTTATATTGCAGCATGGAGGTCGTGCTTTTTTAGCAGATGAAATGGGACTGGGGAAAACGTTGCAGGCCATTGCTGTAGCTGCATGCGTTCGGGATTCGTGGCCTGTTCTCATACTTGCACCATCTGCATTACGTTTGCAATGGGCTTCTATGATTCAACAATGGCTGAATATTCCTTCATCAGATATACTTGTTGTCTTATCTCAAATTGGTGGATCAAATAGAGGAGGTTTCAATATAGTATCTTCAAGTGCCAAAAGCAAAATTCATCTGGATGGACTATTCAACATTATCTCATATGATCTGGTGCCAAAGATGCAAAGTACACTCATGGAGTCTGACTTTAAGGTTGTAATTGCTGATGAGTCACACTTTCTGAAAAATGCCCAGGCAAAGAGAACAACTGCTTCTCTTCCGGTTATAAAGAAAGCACAATATGCAATATTGCTTAGTGGAACTCCTGCTTTATCACGACCAATTGAACTATTCAAACAACTGGAAGCTTTGTATCCTGATGTGTATAAGAATGTTCATGAATATGGTAACCGGTATTGCAAGGGTGGAATGTTTGGAGTTTATCAAGGGGCAAGTAATCATGAGGAATTGCACAATTTGATGAAGGCAACAACGATGATTCGGAGGCTTAAAAAGGATGTTCTTTCTGAACTTCCTGTAAAGCGTAGGCAACAAGTCTTCTTAGACTTGGCCGACAAGGACATGAAGCAAATTAATGCTCTATTTCGTGAGTTGGAGAGGGTAAAGGCCAAAATTAAGGCAGCTAAGTCACAAGAGGAGGCTGAATCACTCAAGTTTTCTAAACAGAATATGATTAACAAGCTTTATACTGATTCTGCGGAAGCCAAGATTCCAGCTGTTCTTGATTATCTTGGAACTGTAATTGAGGCAGGCTGCAAGTTTCTTATATTTGCACACCATCTACCAATGATTGATGCTATACACGAGTTTCTTCTTAAAAAAAAGGTTGCTTGCATCCGGATTGATGGAGGCACACCTTCTGGATCAAGGCAACAATTGGTTACAGAGTTTCAGGAAAAAGATACTATCAAGGCAGCAGTGCTATCTATCAAAGCTGGAGGTGTCGGGTTAACATTGACTGCTGCCAGCACAGTCATCTTTGCAGAATTGTCTTGGACTCCTGGTGATCTAATTCAGGCCGAAGACCGCGTTCATAGAATTGGCCAGGAGTCTTCGGTAAATATATACTACTTGCTTGCCAATGACACAGTTGATGATATCATATGGGACACAGTGCAAAGCAAACTGGAAAATTTGGGGCAGATGCTTGATGGCCATGAGAACACCTTAAAGGTCTCAGATGATCAGCCACCATTGAGTAGCCCTGCAAAGCAAAAAACTCTTGACCATTTTGTCAGAAGATGCGACAACAGCACAGGTGGATTGGAACATCAGTCAACCCCCAAACGTCCCCGGTGGTCAGCACAGCTGGCGACTCTAAACTATGCTGCTGGTGCTGGAAAAGCTGATCGTTTTATCAGATGTTACAATGGTTATGAAGTCTCCCAATAG

Protein sequence

>Msa1219420

MEPEDWDLSGEDLDSLERDAFQKIAQLRSHPSNPPPPSRPQRVDALPQAAARALPTSIKSATNNTTNVEHSKELTKVSVKFFLHSTGNIAAKFQYDQVIVAAFRRIPKSFWNAKERLWLFPLSSLSEAEKVLKDISGYNVQVENLDPLVQRAITAATSVPDLRDRYDKIPSDVESKLLPFQRDGIRFILQHGGRAFLADEMGLGKTLQAIAVAACVRDSWPVLILAPSALRLQWASMIQQWLNIPSSDILVVLSQIGGSNRGGFNIVSSSAKSKIHLDGLFNIISYDLVPKMQSTLMESDFKVVIADESHFLKNAQAKRTTASLPVIKKAQYAILLSGTPALSRPIELFKQLEALYPDVYKNVHEYGNRYCKGGMFGVYQGASNHEELHNLMKATTMIRRLKKDVLSELPVKRRQQVFLDLADKDMKQINALFRELERVKAKIKAAKSQEEAESLKFSKQNMINKLYTDSAEAKIPAVLDYLGTVIEAGCKFLIFAHHLPMIDAIHEFLLKKKVACIRIDGGTPSGSRQQLVTEFQEKDTIKAAVLSIKAGGVGLTLTAASTVIFAELSWTPGDLIQAEDRVHRIGQESSVNIYYLLANDTVDDIIWDTVQSKLENLGQMLDGHENTLKVSDDQPPLSSPAKQKTLDHFVRRCDNSTGGLEHQSTPKRPRWSAQLATLNYAAGAGKADRFIRCYNGYEVSQ*