Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1223280 | G7JJ61 | 89.583 | 144 | 9 | 1 | 1 | 144 | 1 | 138 | 1.28e-87 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1223280 | Msa1263850 | 0.823160 | 1.608289e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1223280 | MtrunA17_Chr4g0074741 | 89.583 | 144 | 9 | 1 | 1 | 144 | 1 | 138 | 2.47e-91 | 261 |
| Msa1223280 | MtrunA17_Chr4g0074761 | 65.035 | 143 | 41 | 3 | 1 | 142 | 1 | 135 | 4.60e-53 | 164 |
| Msa1223280 | MtrunA17_Chr2g0288321 | 47.973 | 148 | 48 | 8 | 1 | 142 | 1 | 125 | 7.73e-27 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGGACTTTGAAATCTTTA+AGG | 0.115808 | 8_1:+87693370 | None:intergenic |
| GTCCGAATTGGCTGAATTAT+TGG | 0.221014 | 8_1:-87693354 | Msa1223280:CDS |
| TTAGAAAGGCTTAGAGCTTC+TGG | 0.263068 | 8_1:-87693413 | Msa1223280:CDS |
| AAGAAGATGGCGAATGAATT+AGG | 0.292703 | 8_1:-87693281 | Msa1223280:CDS |
| CTGCAGAACAAGTTCTTTAT+CGG | 0.325370 | 8_1:-87693256 | Msa1223280:CDS |
| GTTTGCAATTTCATTGTCTC+TGG | 0.335371 | 8_1:+87693223 | None:intergenic |
| CTCAGTAATTAACAACATTC+AGG | 0.361182 | 8_1:+87693164 | None:intergenic |
| TCCGAATTGGCTGAATTATT+GGG | 0.361746 | 8_1:-87693353 | Msa1223280:CDS |
| AAAGATTTCAAAGTCCGAAT+TGG | 0.370993 | 8_1:-87693366 | Msa1223280:CDS |
| GAACGGCATGCCTTTGGCTT+CGG | 0.395190 | 8_1:+87693497 | None:intergenic |
| TGGAGTTTGGTGGAGAAGAC+TGG | 0.395930 | 8_1:-87693553 | Msa1223280:CDS |
| GCAGCATCTTCAATGGAGTT+TGG | 0.400947 | 8_1:-87693566 | Msa1223280:CDS |
| TCCCAATAATTCAGCCAATT+CGG | 0.412446 | 8_1:+87693352 | None:intergenic |
| GATGATGAACGGCATGCCTT+TGG | 0.417430 | 8_1:+87693491 | None:intergenic |
| TTGCAAACAAGCGTGATCAC+TGG | 0.508181 | 8_1:-87693208 | Msa1223280:CDS |
| AATAGATCATTCTCTTTCTG+AGG | 0.510132 | 8_1:+87693434 | None:intergenic |
| TTGCAAGGCAGCATCTTCAA+TGG | 0.513024 | 8_1:-87693573 | Msa1223280:CDS |
| CAAGCGTGATCACTGGAAAC+CGG | 0.513385 | 8_1:-87693201 | Msa1223280:CDS |
| GGAGTTTGGTGGAGAAGACT+GGG | 0.518380 | 8_1:-87693552 | Msa1223280:CDS |
| AAGAGAATGATCTATTAGAA+AGG | 0.530405 | 8_1:-87693427 | Msa1223280:CDS |
| ATGCCGTTCATCATCAAGTA+AGG | 0.531383 | 8_1:-87693483 | Msa1223280:CDS |
| GCATCTTCAATGGAGTTTGG+TGG | 0.547410 | 8_1:-87693563 | Msa1223280:CDS |
| AACAAAGAAGCCGAAGCCAA+AGG | 0.577054 | 8_1:-87693507 | Msa1223280:CDS |
| AAAGCAGATGAAGAAGAAGA+TGG | 0.598887 | 8_1:-87693294 | Msa1223280:CDS |
| ACACCTTACTTGATGATGAA+CGG | 0.600253 | 8_1:+87693480 | None:intergenic |
| TTCTGGAGATGCAAGTGGGA+AGG | 0.602037 | 8_1:-87693396 | Msa1223280:CDS |
| TCAGGAATAGTCTCTAACAC+CGG | 0.606768 | 8_1:+87693182 | None:intergenic |
| AGAGCTTCTGGAGATGCAAG+TGG | 0.611256 | 8_1:-87693401 | Msa1223280:CDS |
| AACTATGGGAAACTGTTGCA+AGG | 0.631074 | 8_1:-87693588 | None:intergenic |
| GAGCTTCTGGAGATGCAAGT+GGG | 0.649801 | 8_1:-87693400 | Msa1223280:CDS |
| GAGTTTGGTGGAGAAGACTG+GGG | 0.753495 | 8_1:-87693551 | Msa1223280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAGAATGATCTATTAGAA+AGG | - | chr8_1:87693329-87693348 | Msa1223280:CDS | 25.0% |
| AATAGATCATTCTCTTTCTG+AGG | + | chr8_1:87693325-87693344 | None:intergenic | 30.0% | |
| !! | TTCGGACTTTGAAATCTTTA+AGG | + | chr8_1:87693389-87693408 | None:intergenic | 30.0% |
| AAAGATTTCAAAGTCCGAAT+TGG | - | chr8_1:87693390-87693409 | Msa1223280:CDS | 30.0% | |
| !! | ACACCTTACTTGATGATGAA+CGG | + | chr8_1:87693279-87693298 | None:intergenic | 35.0% |
| TCCGAATTGGCTGAATTATT+GGG | - | chr8_1:87693403-87693422 | Msa1223280:CDS | 35.0% | |
| TCCCAATAATTCAGCCAATT+CGG | + | chr8_1:87693407-87693426 | None:intergenic | 35.0% | |
| !! | TTCTTCTTCATCTGCTTTTG+CGG | + | chr8_1:87693460-87693479 | None:intergenic | 35.0% |
| AAAGCAGATGAAGAAGAAGA+TGG | - | chr8_1:87693462-87693481 | Msa1223280:CDS | 35.0% | |
| AAGAAGATGGCGAATGAATT+AGG | - | chr8_1:87693475-87693494 | Msa1223280:CDS | 35.0% | |
| CTGCAGAACAAGTTCTTTAT+CGG | - | chr8_1:87693500-87693519 | Msa1223280:CDS | 35.0% | |
| GTTTGCAATTTCATTGTCTC+TGG | + | chr8_1:87693536-87693555 | None:intergenic | 35.0% | |
| ATGCCGTTCATCATCAAGTA+AGG | - | chr8_1:87693273-87693292 | Msa1223280:CDS | 40.0% | |
| TTAGAAAGGCTTAGAGCTTC+TGG | - | chr8_1:87693343-87693362 | Msa1223280:CDS | 40.0% | |
| GTCCGAATTGGCTGAATTAT+TGG | - | chr8_1:87693402-87693421 | Msa1223280:CDS | 40.0% | |
| TCAGGAATAGTCTCTAACAC+CGG | + | chr8_1:87693577-87693596 | None:intergenic | 40.0% | |
| TTGCAAGGCAGCATCTTCAA+TGG | - | chr8_1:87693183-87693202 | Msa1223280:CDS | 45.0% | |
| GCAGCATCTTCAATGGAGTT+TGG | - | chr8_1:87693190-87693209 | Msa1223280:CDS | 45.0% | |
| GCATCTTCAATGGAGTTTGG+TGG | - | chr8_1:87693193-87693212 | Msa1223280:CDS | 45.0% | |
| AACAAAGAAGCCGAAGCCAA+AGG | - | chr8_1:87693249-87693268 | Msa1223280:CDS | 45.0% | |
| TTGCAAACAAGCGTGATCAC+TGG | - | chr8_1:87693548-87693567 | Msa1223280:CDS | 45.0% | |
| ! | TGGAGTTTGGTGGAGAAGAC+TGG | - | chr8_1:87693203-87693222 | Msa1223280:CDS | 50.0% |
| ! | GGAGTTTGGTGGAGAAGACT+GGG | - | chr8_1:87693204-87693223 | Msa1223280:CDS | 50.0% |
| ! | GAGTTTGGTGGAGAAGACTG+GGG | - | chr8_1:87693205-87693224 | Msa1223280:CDS | 50.0% |
| GATGATGAACGGCATGCCTT+TGG | + | chr8_1:87693268-87693287 | None:intergenic | 50.0% | |
| ! | AGAGCTTCTGGAGATGCAAG+TGG | - | chr8_1:87693355-87693374 | Msa1223280:CDS | 50.0% |
| ! | GAGCTTCTGGAGATGCAAGT+GGG | - | chr8_1:87693356-87693375 | Msa1223280:CDS | 50.0% |
| ! | TTCTGGAGATGCAAGTGGGA+AGG | - | chr8_1:87693360-87693379 | Msa1223280:CDS | 50.0% |
| CAAGCGTGATCACTGGAAAC+CGG | - | chr8_1:87693555-87693574 | Msa1223280:CDS | 50.0% | |
| ! | GAACGGCATGCCTTTGGCTT+CGG | + | chr8_1:87693262-87693281 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 87693172 | 87693606 | 87693172 | ID=Msa1223280;Name=Msa1223280 |
| chr8_1 | mRNA | 87693172 | 87693606 | 87693172 | ID=Msa1223280-mRNA-1;Parent=Msa1223280;Name=Msa1223280-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|144 |
| chr8_1 | exon | 87693172 | 87693606 | 87693172 | ID=Msa1223280-mRNA-1:exon:31783;Parent=Msa1223280-mRNA-1 |
| chr8_1 | CDS | 87693172 | 87693606 | 87693172 | ID=Msa1223280-mRNA-1:cds;Parent=Msa1223280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
