Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1263850 | XP_003610421.1 | 88.889 | 144 | 10 | 1 | 1 | 144 | 1 | 138 | 6.25e-87 | 260 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1223280 | Msa1263850 | 0.823160 | 1.608289e-53 | -8.615850e-47 |
| Msa0866400 | Msa1263850 | 0.808508 | 2.991621e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1263850 | MtrunA17_Chr4g0074741 | 88.889 | 144 | 10 | 1 | 1 | 144 | 1 | 138 | 5.75e-91 | 260 |
| Msa1263850 | MtrunA17_Chr4g0074761 | 63.636 | 143 | 43 | 3 | 1 | 142 | 1 | 135 | 1.01e-51 | 160 |
| Msa1263850 | MtrunA17_Chr2g0288321 | 46.575 | 146 | 53 | 7 | 1 | 142 | 1 | 125 | 6.85e-26 | 95.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCGGACTTTGAAATCTTTA+AGG | 0.115808 | 8_2:-67205079 | None:intergenic |
| GTCCGAATTGGCTGAATTAT+TGG | 0.221014 | 8_2:+67205095 | Msa1263850:CDS |
| TTAGAAAGGCTTAGAGCTTC+TGG | 0.263068 | 8_2:+67205036 | Msa1263850:CDS |
| AAGAAGATGGCGAATGAATT+AGG | 0.292703 | 8_2:+67205168 | Msa1263850:CDS |
| CTGCAGAACAAGTTCTTTAT+CGG | 0.325370 | 8_2:+67205193 | Msa1263850:CDS |
| GTTTGCAATTTCATTGTCTC+TGG | 0.335371 | 8_2:-67205226 | None:intergenic |
| TTAAGTAATTAACAACATTC+AGG | 0.343461 | 8_2:-67205285 | None:intergenic |
| TCCGAATTGGCTGAATTATT+GGG | 0.361746 | 8_2:+67205096 | Msa1263850:CDS |
| AAAGATTTCAAAGTCCGAAT+TGG | 0.370993 | 8_2:+67205083 | Msa1263850:CDS |
| GAACGGCATGCCTTTGGCTT+CGG | 0.395190 | 8_2:-67204952 | None:intergenic |
| TAGAGTTTGGTGGAGAAGAC+TGG | 0.400668 | 8_2:+67204896 | Msa1263850:CDS |
| GCAGCATCTTCAATAGAGTT+TGG | 0.409289 | 8_2:+67204883 | Msa1263850:CDS |
| TCCCAATAATTCAGCCAATT+CGG | 0.412446 | 8_2:-67205097 | None:intergenic |
| GATGATGAACGGCATGCCTT+TGG | 0.417430 | 8_2:-67204958 | None:intergenic |
| AATAGATCATTCTCTTTCTG+AGG | 0.510132 | 8_2:-67205015 | None:intergenic |
| TTGCAAACAAGCATGATCAC+TGG | 0.527191 | 8_2:+67205241 | Msa1263850:CDS |
| AAGAGAATGATCTATTAGAA+AGG | 0.530405 | 8_2:+67205022 | Msa1263850:CDS |
| ATGCCGTTCATCATCAAGTA+AGG | 0.531383 | 8_2:+67204966 | Msa1263850:CDS |
| AGAGTTTGGTGGAGAAGACT+GGG | 0.535657 | 8_2:+67204897 | Msa1263850:CDS |
| GCATCTTCAATAGAGTTTGG+TGG | 0.560731 | 8_2:+67204886 | Msa1263850:CDS |
| AACAAAGAAGCCGAAGCCAA+AGG | 0.577054 | 8_2:+67204942 | Msa1263850:CDS |
| TCAGGAATAGTCTCTAACAT+CGG | 0.592945 | 8_2:-67205267 | None:intergenic |
| AAAGCAGATGAAGAAGAAGA+TGG | 0.598887 | 8_2:+67205155 | Msa1263850:CDS |
| ACACCTTACTTGATGATGAA+CGG | 0.600253 | 8_2:-67204969 | None:intergenic |
| TTCTGGAGATGCAAGTGGGA+AGG | 0.602037 | 8_2:+67205053 | Msa1263850:CDS |
| AGAGCTTCTGGAGATGCAAG+TGG | 0.611256 | 8_2:+67205048 | Msa1263850:CDS |
| AACTATGGGAAACTGTTGCA+AGG | 0.631074 | 8_2:+67204861 | None:intergenic |
| GAGCTTCTGGAGATGCAAGT+GGG | 0.649801 | 8_2:+67205049 | Msa1263850:CDS |
| GAGTTTGGTGGAGAAGACTG+GGG | 0.758233 | 8_2:+67204898 | Msa1263850:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAGAATGATCTATTAGAA+AGG | + | chr8_2:67205022-67205041 | Msa1263850:CDS | 25.0% |
| AATAGATCATTCTCTTTCTG+AGG | - | chr8_2:67205018-67205037 | None:intergenic | 30.0% | |
| !! | TTCGGACTTTGAAATCTTTA+AGG | - | chr8_2:67205082-67205101 | None:intergenic | 30.0% |
| AAAGATTTCAAAGTCCGAAT+TGG | + | chr8_2:67205083-67205102 | Msa1263850:CDS | 30.0% | |
| !! | ACACCTTACTTGATGATGAA+CGG | - | chr8_2:67204972-67204991 | None:intergenic | 35.0% |
| TCCGAATTGGCTGAATTATT+GGG | + | chr8_2:67205096-67205115 | Msa1263850:CDS | 35.0% | |
| TCCCAATAATTCAGCCAATT+CGG | - | chr8_2:67205100-67205119 | None:intergenic | 35.0% | |
| !! | TTCTTCTTCATCTGCTTTTG+CGG | - | chr8_2:67205153-67205172 | None:intergenic | 35.0% |
| AAAGCAGATGAAGAAGAAGA+TGG | + | chr8_2:67205155-67205174 | Msa1263850:CDS | 35.0% | |
| AAGAAGATGGCGAATGAATT+AGG | + | chr8_2:67205168-67205187 | Msa1263850:CDS | 35.0% | |
| CTGCAGAACAAGTTCTTTAT+CGG | + | chr8_2:67205193-67205212 | Msa1263850:CDS | 35.0% | |
| GTTTGCAATTTCATTGTCTC+TGG | - | chr8_2:67205229-67205248 | None:intergenic | 35.0% | |
| TCAGGAATAGTCTCTAACAT+CGG | - | chr8_2:67205270-67205289 | None:intergenic | 35.0% | |
| GCAGCATCTTCAATAGAGTT+TGG | + | chr8_2:67204883-67204902 | Msa1263850:CDS | 40.0% | |
| GCATCTTCAATAGAGTTTGG+TGG | + | chr8_2:67204886-67204905 | Msa1263850:CDS | 40.0% | |
| ATGCCGTTCATCATCAAGTA+AGG | + | chr8_2:67204966-67204985 | Msa1263850:CDS | 40.0% | |
| TTAGAAAGGCTTAGAGCTTC+TGG | + | chr8_2:67205036-67205055 | Msa1263850:CDS | 40.0% | |
| GTCCGAATTGGCTGAATTAT+TGG | + | chr8_2:67205095-67205114 | Msa1263850:CDS | 40.0% | |
| TTGCAAACAAGCATGATCAC+TGG | + | chr8_2:67205241-67205260 | Msa1263850:CDS | 40.0% | |
| ! | TAGAGTTTGGTGGAGAAGAC+TGG | + | chr8_2:67204896-67204915 | Msa1263850:CDS | 45.0% |
| ! | AGAGTTTGGTGGAGAAGACT+GGG | + | chr8_2:67204897-67204916 | Msa1263850:CDS | 45.0% |
| AACAAAGAAGCCGAAGCCAA+AGG | + | chr8_2:67204942-67204961 | Msa1263850:CDS | 45.0% | |
| ! | GAGTTTGGTGGAGAAGACTG+GGG | + | chr8_2:67204898-67204917 | Msa1263850:CDS | 50.0% |
| GATGATGAACGGCATGCCTT+TGG | - | chr8_2:67204961-67204980 | None:intergenic | 50.0% | |
| ! | AGAGCTTCTGGAGATGCAAG+TGG | + | chr8_2:67205048-67205067 | Msa1263850:CDS | 50.0% |
| ! | GAGCTTCTGGAGATGCAAGT+GGG | + | chr8_2:67205049-67205068 | Msa1263850:CDS | 50.0% |
| ! | TTCTGGAGATGCAAGTGGGA+AGG | + | chr8_2:67205053-67205072 | Msa1263850:CDS | 50.0% |
| ! | GAACGGCATGCCTTTGGCTT+CGG | - | chr8_2:67204955-67204974 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 67204865 | 67205299 | 67204865 | ID=Msa1263850;Name=Msa1263850 |
| chr8_2 | mRNA | 67204865 | 67205299 | 67204865 | ID=Msa1263850-mRNA-1;Parent=Msa1263850;Name=Msa1263850-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|144 |
| chr8_2 | exon | 67204865 | 67205299 | 67204865 | ID=Msa1263850-mRNA-1:exon:22064;Parent=Msa1263850-mRNA-1 |
| chr8_2 | CDS | 67204865 | 67205299 | 67204865 | ID=Msa1263850-mRNA-1:cds;Parent=Msa1263850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
