AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1225930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225930 MtrunA17_Chr3g0101321 92.771 415 30 0 1 415 506 920 0.0 790
Msa1225930 MtrunA17_Chr3g0112511 54.479 413 184 3 1 413 505 913 4.49e-134 407
Msa1225930 MtrunA17_Chr3g0101271 54.815 405 172 4 1 400 488 886 3.58e-127 388
Msa1225930 MtrunA17_Chr3g0101241 54.815 405 172 4 1 400 488 886 1.03e-126 387
Msa1225930 MtrunA17_Chr3g0100731 50.000 414 202 4 1 413 269 678 1.94e-122 370
Msa1225930 MtrunA17_Chr3g0101201 52.542 413 178 9 1 400 513 920 6.75e-117 362
Msa1225930 MtrunA17_Chr3g0100791 50.000 396 190 4 1 394 49 438 3.60e-114 341
Msa1225930 MtrunA17_Chr3g0100701 51.613 403 183 7 1 401 344 736 1.02e-113 349
Msa1225930 MtrunA17_Chr3g0101331 53.627 386 165 7 1 374 522 905 1.46e-113 353
Msa1225930 MtrunA17_Chr3g0080741 44.365 417 216 3 1 405 518 930 1.06e-110 347
Msa1225930 MtrunA17_Chr1g0189281 52.267 375 167 7 1 366 2 373 2.48e-110 329
Msa1225930 MtrunA17_Chr3g0080601 46.341 410 204 5 1 398 515 920 2.78e-109 343
Msa1225930 MtrunA17_Chr3g0101251 50.242 414 186 6 1 400 475 882 8.92e-109 340
Msa1225930 MtrunA17_Chr3g0080631 44.844 417 214 4 1 405 471 883 7.98e-108 338
Msa1225930 MtrunA17_Chr3g0100851 49.495 396 192 4 1 394 390 779 1.82e-107 334
Msa1225930 MtrunA17_Chr3g0080771 46.117 412 205 6 1 400 516 922 2.31e-107 338
Msa1225930 MtrunA17_Chr3g0100831 47.952 415 205 6 1 411 509 916 2.43e-107 337
Msa1225930 MtrunA17_Chr3g0101391 50.130 385 180 8 1 375 196 578 4.31e-107 327
Msa1225930 MtrunA17_Chr3g0080791 47.573 412 199 5 1 400 516 922 3.19e-106 335
Msa1225930 MtrunA17_Chr3g0101221 49.517 414 191 8 1 401 515 923 2.10e-105 332
Msa1225930 MtrunA17_Chr3g0100961 48.309 414 193 9 1 411 514 909 4.17e-105 331
Msa1225930 MtrunA17_Chr3g0094301 45.122 410 209 4 1 398 516 921 1.20e-104 330
Msa1225930 MtrunA17_Chr7g0229761 45.122 410 209 4 1 398 516 921 1.20e-104 330
Msa1225930 MtrunA17_Chr3g0101231 50.361 415 188 8 1 401 519 929 8.51e-104 328
Msa1225930 MtrunA17_Chr3g0101361 48.364 428 188 7 1 399 344 767 1.86e-103 323
Msa1225930 MtrunA17_Chr1g0189351 50.891 393 170 8 1 374 511 899 3.62e-103 326
Msa1225930 MtrunA17_Chr3g0080721 43.066 411 216 5 1 398 252 657 4.38e-103 320
Msa1225930 MtrunA17_Chr3g0080751 42.857 413 220 4 1 401 517 925 8.68e-101 320
Msa1225930 MtrunA17_Chr5g0444301 52.646 378 167 5 1 370 71 444 7.45e-100 305
Msa1225930 MtrunA17_Chr3g0101011 46.845 412 197 10 1 398 528 931 3.33e-98 313
Msa1225930 MtrunA17_Chr3g0101181 49.630 405 186 7 1 389 519 921 5.62e-98 312
Msa1225930 MtrunA17_Chr3g0081391 42.579 411 219 5 1 398 515 921 2.17e-94 303
Msa1225930 MtrunA17_Chr3g0080731 42.195 410 221 4 1 398 516 921 8.96e-94 301
Msa1225930 MtrunA17_Chr1g0151481 44.681 423 216 8 1 410 517 934 2.20e-91 295
Msa1225930 MtrunA17_Chr3g0081191 41.289 419 227 6 1 405 511 924 8.94e-91 293
Msa1225930 MtrunA17_Chr3g0080651 43.765 409 215 5 1 398 432 836 1.07e-90 292
Msa1225930 MtrunA17_Chr3g0081341 41.748 412 223 4 1 399 579 986 1.54e-88 289
Msa1225930 MtrunA17_Chr3g0081371 42.029 414 220 8 1 399 520 928 2.18e-87 285
Msa1225930 MtrunA17_Chr3g0081211 43.309 411 216 6 1 398 514 920 2.92e-87 284
Msa1225930 MtrunA17_Chr3g0081361 41.019 412 225 6 1 398 520 927 2.49e-86 283
Msa1225930 MtrunA17_Chr3g0100801 47.962 319 148 3 1 318 470 771 3.92e-80 262
Msa1225930 MtrunA17_Chr3g0100911 58.009 231 96 1 116 346 1 230 5.30e-80 246
Msa1225930 MtrunA17_Chr3g0081181 40.389 411 228 4 1 398 499 905 5.35e-73 245
Msa1225930 MtrunA17_Chr5g0409991 35.581 430 247 11 5 413 499 919 2.30e-66 227
Msa1225930 MtrunA17_Chr5g0410021 35.581 430 247 11 5 413 501 921 1.25e-65 225
Msa1225930 MtrunA17_Chr3g0084361 48.148 243 122 3 156 398 1 239 1.82e-65 210
Msa1225930 MtrunA17_Chr3g0100761 52.466 223 104 1 1 221 471 693 4.92e-64 218
Msa1225930 MtrunA17_Chr3g0100971 52.466 223 104 1 1 221 511 733 7.89e-64 218
Msa1225930 MtrunA17_Chr5g0409971 36.175 434 247 11 1 413 500 924 1.77e-63 219
Msa1225930 MtrunA17_Chr3g0100681 47.328 262 129 5 1 259 327 582 9.22e-63 212
Msa1225930 MtrunA17_Chr5g0410001 35.267 431 250 14 1 411 500 921 9.98e-63 217
Msa1225930 MtrunA17_Chr5g0410031 36.176 387 224 10 46 414 548 929 5.51e-59 207
Msa1225930 MtrunA17_Chr3g0081201 41.429 210 119 3 192 400 2 208 1.31e-44 154
Msa1225930 MtrunA17_Chr1g0149071 52.410 166 76 3 156 320 289 452 2.23e-39 146
Msa1225930 MtrunA17_Chr3g0101161 48.990 198 85 4 206 399 2 187 1.09e-37 135
Msa1225930 MtrunA17_Chr2g0299001 30.411 365 227 12 47 400 575 923 7.30e-33 131
Msa1225930 MtrunA17_Chr1g0189321 51.493 134 57 3 1 128 108 239 7.46e-30 115
Msa1225930 MtrunA17_Chr1g0189331 56.364 110 46 2 163 271 1 109 7.98e-27 103
Msa1225930 MtrunA17_Chr7g0219851 28.226 372 247 9 45 401 571 937 1.59e-25 109
Msa1225930 MtrunA17_Chr1g0197241 29.936 314 207 5 71 374 1223 1533 5.18e-25 108
Msa1225930 MtrunA17_Chr5g0404741 31.395 344 214 10 65 399 576 906 2.08e-24 105
Msa1225930 MtrunA17_Chr3g0100981 45.000 140 75 1 265 404 4 141 2.17e-23 95.1
Msa1225930 MtrunA17_Chr3g0100771 45.000 140 75 1 265 404 4 141 2.17e-23 95.1
Msa1225930 MtrunA17_Chr1g0160871 40.881 159 74 6 123 269 1 151 3.97e-23 94.7
Msa1225930 MtrunA17_Chr1g0197201 29.518 332 205 8 71 377 1021 1348 9.16e-23 101
Msa1225930 MtrunA17_Chr1g0197181 27.907 344 212 9 71 384 182 519 1.33e-22 99.8
Msa1225930 MtrunA17_Chr1g0189341 51.515 99 46 1 289 387 1 97 1.22e-21 89.7
Msa1225930 MtrunA17_Chr3g0084351 41.228 114 67 0 1 114 441 554 1.23e-21 97.1
Msa1225930 MtrunA17_Chr3g0100691 46.617 133 69 1 265 397 4 134 1.46e-21 90.1
Msa1225930 MtrunA17_Chr3g0101211 45.098 102 56 0 1 102 302 403 1.57e-19 89.7
Msa1225930 MtrunA17_Chr4g0037421 27.764 407 241 18 2 370 561 952 1.23e-16 82.4
Msa1225930 MtrunA17_Chr5g0433771 25.337 371 212 11 37 401 543 854 4.03e-16 80.9
Msa1225930 MtrunA17_Chr3g0085451 27.577 388 243 17 11 370 485 862 1.10e-14 76.3
Msa1225930 MtrunA17_Chr3g0081841 40.196 102 58 1 194 295 383 481 1.22e-14 75.5
Msa1225930 MtrunA17_Chr3g0082791 27.152 302 199 11 11 292 510 810 2.54e-14 75.1
Msa1225930 MtrunA17_Chr7g0257541 25.840 387 211 17 22 395 534 857 9.14e-14 73.6
Msa1225930 MtrunA17_Chr7g0248221 26.359 368 213 10 30 395 530 841 9.34e-14 73.6
Msa1225930 MtrunA17_Chr3g0085801 29.741 232 142 9 59 274 532 758 1.25e-13 73.2
Msa1225930 MtrunA17_Chr8g0352831 28.912 294 173 12 10 273 517 804 1.48e-13 72.8
Msa1225930 MtrunA17_Chr5g0410351 28.383 303 185 10 58 328 530 832 1.61e-13 72.8
Msa1225930 MtrunA17_Chr3g0087731 25.887 282 185 8 11 273 302 578 1.73e-13 72.4
Msa1225930 MtrunA17_Chr3g0085561 27.692 260 158 10 48 283 530 783 2.27e-13 72.4
Msa1225930 MtrunA17_Chr5g0410301 26.829 328 206 13 58 356 531 853 2.84e-13 72.0
Msa1225930 MtrunA17_Chr5g0410261 27.211 294 187 9 58 328 562 851 5.06e-13 71.2
Msa1225930 MtrunA17_Chr5g0410481 27.410 332 206 12 58 356 520 849 6.88e-13 70.9
Msa1225930 MtrunA17_Chr8g0344071 26.284 331 196 8 75 381 846 1152 9.58e-13 70.5
Msa1225930 MtrunA17_Chr5g0446611 27.684 354 192 19 19 355 515 821 1.01e-12 70.1
Msa1225930 MtrunA17_Chr3g0084051 29.339 242 158 7 11 240 509 749 1.47e-12 69.7
Msa1225930 MtrunA17_Chr3g0085331 32.000 150 94 4 48 192 561 707 2.36e-12 69.3
Msa1225930 MtrunA17_Chr3g0087581 29.054 296 178 11 5 274 487 776 3.32e-12 68.6
Msa1225930 MtrunA17_Chr3g0085471 32.278 158 102 4 37 192 543 697 4.16e-12 68.6
Msa1225930 MtrunA17_Chr3g0089211 28.452 239 151 8 50 273 601 834 4.91e-12 68.2
Msa1225930 MtrunA17_Chr4g0058541 29.717 212 140 5 74 284 228 431 6.05e-12 67.4
Msa1225930 MtrunA17_Chr5g0430341 24.654 434 249 18 12 404 44 440 6.45e-12 67.4
Msa1225930 MtrunA17_Chr3g0089251 29.289 239 149 9 50 273 583 816 6.59e-12 67.8
Msa1225930 MtrunA17_Chr5g0410161 26.549 339 209 12 58 356 677 1015 9.21e-12 67.4
Msa1225930 MtrunA17_Chr3g0087541 26.557 305 190 12 17 292 518 817 9.55e-12 67.4
Msa1225930 MtrunA17_Chr5g0415481 25.945 397 250 19 12 375 73 458 1.14e-11 66.2
Msa1225930 MtrunA17_Chr5g0431051 26.238 404 245 19 12 386 501 880 1.53e-11 66.6
Msa1225930 MtrunA17_Chr5g0407461 23.235 340 182 5 70 399 275 545 1.64e-11 66.2
Msa1225930 MtrunA17_Chr3g0087701 26.269 335 174 12 5 292 489 797 2.03e-11 66.2
Msa1225930 MtrunA17_Chr5g0429961 26.540 422 253 20 12 404 500 893 2.20e-11 65.9
Msa1225930 MtrunA17_Chr3g0082881 29.304 273 154 12 60 299 551 817 2.61e-11 65.9
Msa1225930 MtrunA17_Chr8g0370111 24.913 289 158 5 86 374 280 509 3.08e-11 65.1
Msa1225930 MtrunA17_Chr5g0414161 26.730 318 207 12 24 322 5 315 3.40e-11 64.7
Msa1225930 MtrunA17_Chr3g0089441 26.282 312 192 13 87 367 98 402 3.82e-11 65.1
Msa1225930 MtrunA17_Chr1g0183231 25.176 425 237 17 9 377 97 496 4.78e-11 65.1
Msa1225930 MtrunA17_Chr3g0085651 29.097 299 172 12 5 274 496 783 5.58e-11 64.7
Msa1225930 MtrunA17_Chr5g0415471 28.086 324 197 16 12 307 507 822 6.04e-11 64.7
Msa1225930 MtrunA17_Chr3g0085501 34.074 135 85 3 59 192 576 707 6.76e-11 64.7
Msa1225930 MtrunA17_Chr3g0085711 28.402 169 116 3 59 223 122 289 8.73e-11 63.9
Msa1225930 MtrunA17_Chr3g0091121 25.610 328 205 12 72 368 188 507 8.88e-11 63.9
Msa1225930 MtrunA17_Chr5g0413811 25.240 416 247 14 12 395 280 663 9.91e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1225930 AT3G07040.1 28.726 369 243 7 42 401 555 912 1.03e-34 137
Msa1225930 AT5G48620.6 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G48620.1 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G48620.4 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G48620.2 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G48620.3 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G48620.5 28.682 387 248 11 28 400 531 903 1.39e-27 115
Msa1225930 AT5G43470.3 27.792 403 259 13 13 400 518 903 1.45e-25 110
Msa1225930 AT5G43470.2 27.792 403 259 13 13 400 518 903 1.45e-25 110
Msa1225930 AT5G43470.4 27.792 403 259 13 13 400 518 903 1.45e-25 110
Msa1225930 AT5G43470.1 27.792 403 259 13 13 400 518 903 1.45e-25 110
Msa1225930 AT5G35450.2 27.671 365 238 10 48 401 544 893 5.71e-24 105
Msa1225930 AT5G35450.1 27.671 365 238 10 48 401 544 893 5.71e-24 105
Msa1225930 AT1G58400.1 25.419 358 247 8 51 400 550 895 9.39e-22 98.6
Msa1225930 AT1G58400.3 25.419 358 247 8 51 400 560 905 1.05e-21 98.2
Msa1225930 AT1G58400.2 25.419 358 247 8 51 400 560 905 1.05e-21 98.2
Msa1225930 AT1G59780.2 27.164 335 215 9 73 400 569 881 7.02e-20 92.8
Msa1225930 AT1G59780.1 27.164 335 215 9 73 400 574 886 7.13e-20 92.8
Msa1225930 AT1G53350.1 25.849 383 262 9 28 400 531 901 9.53e-20 92.4
Msa1225930 AT1G59620.2 23.724 392 264 8 15 400 377 739 5.31e-19 89.7
Msa1225930 AT1G58390.1 26.393 341 222 9 69 400 581 901 1.41e-18 88.6
Msa1225930 AT1G58390.2 26.393 341 222 9 69 400 581 901 1.41e-18 88.6
Msa1225930 AT1G58410.1 25.824 364 242 11 48 400 551 897 1.42e-18 88.6
Msa1225930 AT1G58410.4 25.824 364 242 11 48 400 551 897 1.42e-18 88.6
Msa1225930 AT1G58410.2 25.824 364 242 11 48 400 551 897 1.42e-18 88.6
Msa1225930 AT1G58410.3 25.824 364 242 11 48 400 519 865 1.98e-18 88.2
Msa1225930 AT1G59124.2 23.799 458 238 14 43 401 551 996 9.32e-14 73.9
Msa1225930 AT1G58807.1 23.799 458 238 14 43 401 551 996 9.32e-14 73.9
Msa1225930 AT3G46710.1 27.891 294 177 10 45 322 531 805 9.58e-14 73.9
Msa1225930 AT1G27170.2 29.592 294 194 4 82 374 752 1033 2.31e-13 72.8
Msa1225930 AT1G27170.1 29.592 294 194 4 82 374 752 1033 3.07e-13 72.4
Msa1225930 AT1G59218.1 24.742 388 218 16 69 401 681 1049 6.56e-12 68.2
Msa1225930 AT1G59218.2 24.742 388 218 16 69 401 681 1049 6.56e-12 68.2
Msa1225930 AT1G59218.3 24.742 388 218 16 69 401 681 1049 6.56e-12 68.2
Msa1225930 AT1G58848.1 24.742 388 218 16 69 401 681 1049 6.56e-12 68.2
Msa1225930 AT1G58848.2 24.742 388 218 16 69 401 681 1049 6.56e-12 68.2
Msa1225930 AT2G19330.1 29.279 222 125 6 77 297 88 278 3.11e-11 65.1
Msa1225930 AT1G69550.1 30.743 296 175 12 74 356 934 1212 9.40e-11 64.7

Find 144 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGGATTCCAATTTAAT+TGG 0.106217 8_1:+91613178 Msa1225930:five_prime_UTR
AGCTTCAAGTTCTCAGTATT+TGG 0.114999 8_1:+91614573 Msa1225930:CDS
ACAGATTGTTCTTCAGTTAA+AGG 0.117375 8_1:-91612962 None:intergenic
CTCTTTAATCCCAATTAAAT+TGG 0.143314 8_1:-91613188 None:intergenic
CCATTGGATCATCTGTTATT+TGG 0.144805 8_1:-91614310 None:intergenic
AACAAATTCTAATGATTTAA+TGG 0.158680 8_1:+91613581 Msa1225930:CDS
AGATCATGTTTGTTGTATTT+TGG 0.159990 8_1:+91613250 Msa1225930:five_prime_UTR
TTGAAGCAGTTAAGAGAATT+AGG 0.182632 8_1:+91614047 Msa1225930:CDS
CTAAGCTTGCTAATCTCTTT+TGG 0.213372 8_1:-91613882 None:intergenic
CATCCAAAGTCTCTAGGTTT+TGG 0.224491 8_1:-91613833 None:intergenic
GACTCAATTTCATAGTCTTC+TGG 0.225664 8_1:-91613280 None:intergenic
ATCAAATTGCCTATATTTCT+AGG 0.246259 8_1:-91613744 None:intergenic
TGGAGCATGCCAATGGATTT+TGG 0.256432 8_1:-91613813 None:intergenic
GATATATCAATGAGCGCTTT+TGG 0.261544 8_1:-91613028 None:intergenic
AGTTATTGATTCAAGATTCT+TGG 0.269381 8_1:+91615218 Msa1225930:three_prime_UTR
CATTGGATCATCTGTTATTT+GGG 0.283580 8_1:-91614309 None:intergenic
CCAGTTTAATTATACATAAC+TGG 0.307205 8_1:+91615443 Msa1225930:three_prime_UTR
TCTCAATCATACAGTATAGA+AGG 0.317497 8_1:+91612662 Msa1225930:five_prime_UTR
TAATATATGATTTGTTGTTC+TGG 0.320910 8_1:+91615383 Msa1225930:three_prime_UTR
ACTGACTGTCTTCACTAATA+TGG 0.331270 8_1:-91613521 None:intergenic
ATCTCTACGATTAACATTGA+TGG 0.337298 8_1:+91613420 Msa1225930:five_prime_UTR
GCTTCAAGTTCTCAGTATTT+GGG 0.337826 8_1:+91614574 Msa1225930:CDS
CCAGTTATGTATAATTAAAC+TGG 0.346044 8_1:-91615443 None:intergenic
GATTCTGTTCCTAGAAATAT+AGG 0.349646 8_1:+91613735 Msa1225930:CDS
AGAGTTGATTAATAGAAGTT+TGG 0.355272 8_1:+91613392 Msa1225930:five_prime_UTR
ATTAAATTGAAGGATGGTAT+TGG 0.360658 8_1:+91613942 Msa1225930:CDS
GCTGTAATTGATAATCAAAA+TGG 0.362248 8_1:+91612854 Msa1225930:five_prime_UTR
TGTTGTCTCTGTGGTGGGAA+TGG 0.364889 8_1:+91612550 Msa1225930:five_prime_UTR
GTACTGTCTTTCTCTAATAA+TGG 0.365278 8_1:+91615161 Msa1225930:three_prime_UTR
CTTCGAGATCTTAGACTGTA+TGG 0.366172 8_1:+91614218 Msa1225930:CDS
ATGCTTTGCACAAATTCATT+TGG 0.366795 8_1:-91614602 None:intergenic
TATTGAAAGCTCACATGTTC+GGG 0.370732 8_1:+91613608 Msa1225930:CDS
ACTCAATTTCATAGTCTTCT+GGG 0.375980 8_1:-91613279 None:intergenic
AATGTCTTTCATTAAATTGA+AGG 0.379051 8_1:+91613932 Msa1225930:CDS
ACAAGGATTCCAATTTAATT+GGG 0.388590 8_1:+91613179 Msa1225930:five_prime_UTR
GTATTGAAAGCTCACATGTT+CGG 0.394830 8_1:+91613607 Msa1225930:CDS
GTTGTCTCTGTGGTGGGAAT+GGG 0.398274 8_1:+91612551 Msa1225930:five_prime_UTR
ACGGCGGTATTGTTTACTTA+AGG 0.399757 8_1:-91613547 None:intergenic
AGAAGTAGTAGAACTATTCC+AGG 0.402773 8_1:+91614013 Msa1225930:CDS
TGTGGTGGGAATGGGAGGGC+AGG 0.410113 8_1:+91612559 Msa1225930:five_prime_UTR
GTCTCTGTGGTGGGAATGGG+AGG 0.413310 8_1:+91612554 Msa1225930:five_prime_UTR
ATCTTGAATCAATAACTTTG+AGG 0.418464 8_1:-91615212 None:intergenic
TGTGGAGTTGTTTAATAAGA+AGG 0.426046 8_1:+91612982 Msa1225930:five_prime_UTR
GCCTCAGTATACTACGGAAA+TGG 0.434100 8_1:+91612724 Msa1225930:five_prime_UTR
GTTAATTGGCAGGTGGTGCC+TGG 0.435844 8_1:+91615063 Msa1225930:intron
CACTTGTTGTCACTCTATAT+CGG 0.439018 8_1:+91614353 Msa1225930:CDS
AAATCTTGTTGAGTTAAAGT+TGG 0.439534 8_1:+91614280 Msa1225930:CDS
TAGATATTTCTAATTTGTGC+AGG 0.440127 8_1:+91615475 Msa1225930:three_prime_UTR
GACACTTTGATTGTCGTGTA+TGG 0.441332 8_1:+91612630 Msa1225930:five_prime_UTR
TTTGATAGCAAGGAGGTTAG+TGG 0.443884 8_1:+91612608 Msa1225930:five_prime_UTR
AAACATGATCTAAGATAGTA+AGG 0.449915 8_1:-91613238 None:intergenic
GCTCCAAAACCTAGAGACTT+TGG 0.453614 8_1:+91613830 Msa1225930:CDS
GGTAGGTACAGTCTTGAGTT+TGG 0.462876 8_1:-91614531 None:intergenic
CTCTTAGAAAGCTCGAGTTA+TGG 0.469545 8_1:+91614501 Msa1225930:CDS
AAGGGTTTGTGAAAAGGAAA+AGG 0.470584 8_1:+91613332 Msa1225930:five_prime_UTR
CAAAGCATTGCTCCAAATGA+AGG 0.475354 8_1:+91614617 Msa1225930:CDS
GAAACTACTTGCAGCAAAAG+AGG 0.477642 8_1:+91612774 Msa1225930:five_prime_UTR
AGGGTTTGTGAAAAGGAAAA+GGG 0.482559 8_1:+91613333 Msa1225930:five_prime_UTR
TAGCTGAAGGGTTTGTGAAA+AGG 0.483374 8_1:+91613326 Msa1225930:five_prime_UTR
CTAGAGAAAGATTGATCGGC+TGG 0.496540 8_1:+91612502 Msa1225930:five_prime_UTR
CTTGAAGCTTCTCTAAGTGT+TGG 0.497520 8_1:-91614559 None:intergenic
CTAGAGACTTTGGATGTAAG+AGG 0.500048 8_1:+91613840 Msa1225930:CDS
ATTGGCAGGTGGTGCCTGGT+TGG 0.504456 8_1:+91615067 Msa1225930:intron
CTCTTACATCCAAAGTCTCT+AGG 0.508935 8_1:-91613839 None:intergenic
ATCGGCCACCATGGTTATGA+AGG 0.512185 8_1:+91614371 Msa1225930:CDS
AGTACACTTGGAGCAATCAC+TGG 0.513368 8_1:-91615093 None:intergenic
TATTTCTAGGAACAGAATCA+AGG 0.515266 8_1:-91613731 None:intergenic
ATTGATCGGCTGGTTGGAAA+AGG 0.516058 8_1:+91612512 Msa1225930:five_prime_UTR
TATACTACGGAAATGGATAG+AGG 0.518537 8_1:+91612731 Msa1225930:five_prime_UTR
TGATCCTTTGTCAATGATGA+TGG 0.519128 8_1:-91614471 None:intergenic
AAACTAAAGGAACTAACCAT+TGG 0.519283 8_1:+91614428 Msa1225930:CDS
CGCACTGTTGTCTCTGTGGT+GGG 0.519704 8_1:+91612545 Msa1225930:five_prime_UTR
TACAAAGGGCACATGCTGAA+TGG 0.521415 8_1:-91614654 None:intergenic
AGAAAGATTGATCGGCTGGT+TGG 0.521429 8_1:+91612506 Msa1225930:five_prime_UTR
CAACTCATGCACTTTGACAA+AGG 0.521741 8_1:-91612936 None:intergenic
CGAACAGATTTGCCACCAGC+AGG 0.528516 8_1:-91614686 None:intergenic
CTTCGTCAGTGGGTAGCTGA+AGG 0.529366 8_1:+91613313 Msa1225930:five_prime_UTR
GCAGGATAAAACTCTACAAA+GGG 0.529597 8_1:-91614668 None:intergenic
CCACTAGCAATTGTTGCCAT+CGG 0.530538 8_1:+91613082 Msa1225930:five_prime_UTR
ATGGAGGAAATTGATGTTGT+GGG 0.530838 8_1:+91612468 Msa1225930:five_prime_UTR
CAAATGAAGGAAAAGAGCAC+TGG 0.533037 8_1:+91614630 Msa1225930:CDS
AAAATGCAATCGAGTGGAAG+AGG 0.536610 8_1:+91613128 Msa1225930:five_prime_UTR
CTTCTCTAAGTGTTGGATGC+CGG 0.536980 8_1:-91614552 None:intergenic
AGTCTAAGATCTCGAAGCAT+TGG 0.537507 8_1:-91614212 None:intergenic
TGTAGCTTTGGAATCCACTC+TGG 0.545672 8_1:-91614257 None:intergenic
GTGGTGGGAATGGGAGGGCA+GGG 0.546309 8_1:+91612560 Msa1225930:five_prime_UTR
GCAATCACTGGAAGCCAACC+AGG 0.547086 8_1:-91615081 None:intergenic
TGGAGGGTTTCACAAACTAA+AGG 0.549692 8_1:+91614415 Msa1225930:CDS
TTCGTCAGTGGGTAGCTGAA+GGG 0.551594 8_1:+91613314 Msa1225930:five_prime_UTR
CAGTATAGAAGGGTTGTTGA+GGG 0.552234 8_1:+91612673 Msa1225930:five_prime_UTR
ACTCAATGAGATGCAACACT+TGG 0.553831 8_1:+91614115 Msa1225930:CDS
TCTTTCATTAAATTGAAGGA+TGG 0.554087 8_1:+91613936 Msa1225930:CDS
CCAAATAACAGATGATCCAA+TGG 0.554575 8_1:+91614310 Msa1225930:CDS
AAAAGCCTTCCAAAATCCAT+TGG 0.555015 8_1:+91613804 Msa1225930:CDS
GCGCACTGTTGTCTCTGTGG+TGG 0.556696 8_1:+91612544 Msa1225930:five_prime_UTR
CTAAGATCTCGAAGCATTGG+TGG 0.559163 8_1:-91614209 None:intergenic
GTCTTTGCCTCAGTATACTA+CGG 0.562009 8_1:+91612718 Msa1225930:five_prime_UTR
TCCTCCATGTGAACCAATCG+AGG 0.564468 8_1:-91612453 None:intergenic
AGAAAGCTACTACATCTTCG+AGG 0.572349 8_1:+91613903 Msa1225930:CDS
AATTTGTTTCTATTGATAGA+CGG 0.573415 8_1:-91613566 None:intergenic
TTCATACATGATACATTACA+AGG 0.574701 8_1:-91615263 None:intergenic
GCAGTTAAGAGAATTAGGCT+TGG 0.577236 8_1:+91614052 Msa1225930:CDS
CATGGAGGAAATTGATGTTG+TGG 0.577832 8_1:+91612467 Msa1225930:five_prime_UTR
TACTTGAGGACAAAGAACTA+AGG 0.578702 8_1:+91614710 Msa1225930:CDS
TTTAACTGAAGAACAATCTG+TGG 0.580854 8_1:+91612964 Msa1225930:five_prime_UTR
TTCGAGATCTTAGACTGTAT+GGG 0.581887 8_1:+91614219 Msa1225930:CDS
GAGTCTAGAGAAAGATTGAT+CGG 0.583252 8_1:+91612498 Msa1225930:five_prime_UTR
CAAGGAAGATGAATTTCCAA+TGG 0.583376 8_1:-91614444 None:intergenic
ATTTCTAGGAACAGAATCAA+GGG 0.586905 8_1:-91613730 None:intergenic
CTCAATCATACAGTATAGAA+GGG 0.588942 8_1:+91612663 Msa1225930:five_prime_UTR
TCTCTGTGGTGGGAATGGGA+GGG 0.589635 8_1:+91612555 Msa1225930:five_prime_UTR
GTTCGTCTTAGAAGAGAGCA+TGG 0.594697 8_1:+91614074 Msa1225930:CDS
TAAAGATAAAAGACCTCCGA+TGG 0.596937 8_1:-91613098 None:intergenic
AGCAGGATAAAACTCTACAA+AGG 0.597809 8_1:-91614669 None:intergenic
GCAAATCTGTTCGATACTTG+AGG 0.601466 8_1:+91614696 Msa1225930:CDS
AACTTTCACCTTCATAACCA+TGG 0.601685 8_1:-91614379 None:intergenic
AACGGTTGTACAAGTACACT+TGG 0.606675 8_1:-91615105 None:intergenic
GTCAATGATGATGGAACACA+AGG 0.609161 8_1:-91614462 None:intergenic
AGTTAGAGAAGCTGCCAGAG+TGG 0.609725 8_1:+91614243 Msa1225930:CDS
TGTTCCATCATCATTGACAA+AGG 0.609943 8_1:+91614467 Msa1225930:CDS
CCCTCGATTGGTTCACATGG+AGG 0.612781 8_1:+91612452 Msa1225930:five_prime_UTR
CTAAGATACTACTTCGTCAG+TGG 0.613003 8_1:+91613302 Msa1225930:five_prime_UTR
ACAGTATAGAAGGGTTGTTG+AGG 0.614945 8_1:+91612672 Msa1225930:five_prime_UTR
GGGTTTGTGAAAAGGAAAAG+GGG 0.616412 8_1:+91613334 Msa1225930:five_prime_UTR
AAACTCAAGACTGTACCTAC+CGG 0.622920 8_1:+91614533 Msa1225930:CDS
TGACCCTCGATTGGTTCACA+TGG 0.623455 8_1:+91612449 Msa1225930:five_prime_UTR
TAAATGGTATGACCCTCGAT+TGG 0.626326 8_1:+91612440 Msa1225930:five_prime_UTR
TTGGAACAGACATGCCGCAA+CGG 0.626521 8_1:-91615123 None:intergenic
CTAGCAATTGTTGCCATCGG+AGG 0.628134 8_1:+91613085 Msa1225930:five_prime_UTR
AGAGCGCACTGTTGTCTCTG+TGG 0.628766 8_1:+91612541 Msa1225930:five_prime_UTR
TAAGATACTACTTCGTCAGT+GGG 0.630857 8_1:+91613303 Msa1225930:five_prime_UTR
ATACTACGGAAATGGATAGA+GGG 0.636806 8_1:+91612732 Msa1225930:five_prime_UTR
TCTAAGTGTTGGATGCCGGT+AGG 0.638966 8_1:-91614548 None:intergenic
TCACTCTATATCGGCCACCA+TGG 0.646259 8_1:+91614362 Msa1225930:CDS
TCTACTCTTAGAACTAAACA+AGG 0.646635 8_1:+91613162 Msa1225930:five_prime_UTR
GAAGACATTAGAAGAAGTTG+CGG 0.654596 8_1:+91613356 Msa1225930:five_prime_UTR
CCTCCATGTGAACCAATCGA+GGG 0.658471 8_1:-91612452 None:intergenic
TCCATTTCCGTAGTATACTG+AGG 0.663072 8_1:-91612725 None:intergenic
CCGATGGCAACAATTGCTAG+TGG 0.670331 8_1:-91613082 None:intergenic
TAGAGGGTCATTAATCGATG+AGG 0.673153 8_1:+91612748 Msa1225930:five_prime_UTR
GTGTACTTGTACAACCGTTG+CGG 0.677191 8_1:+91615109 Msa1225930:three_prime_UTR
TTGTTTCTATTGATAGACGG+CGG 0.697782 8_1:-91613563 None:intergenic
GTAAGATATTTATAACAACA+AGG 0.706788 8_1:+91612879 Msa1225930:five_prime_UTR
TTTCACCTTCATAACCATGG+TGG 0.730892 8_1:-91614376 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAATTCTAATGATTTAA+TGG + chr8_1:91613581-91613600 Msa1225930:CDS 15.0%
!!! AATATGTATTGATAATCATT+TGG - chr8_1:91614751-91614770 None:intergenic 15.0%
!!! AGTAATAATTTGTTGTTAAT+TGG + chr8_1:91615049-91615068 Msa1225930:intron 15.0%
!!! GTACATTATTATTTTTTCAT+TGG - chr8_1:91615145-91615164 None:intergenic 15.0%
!!! TTTTCTTATTCTAAAAGAAA+AGG - chr8_1:91615350-91615369 None:intergenic 15.0%
!! GTAAGATATTTATAACAACA+AGG + chr8_1:91612879-91612898 Msa1225930:five_prime_UTR 20.0%
!! GATATTGTTAAAAAATGCAA+AGG + chr8_1:91613055-91613074 Msa1225930:five_prime_UTR 20.0%
!!! AATTTGTTTCTATTGATAGA+CGG - chr8_1:91613569-91613588 None:intergenic 20.0%
!! AATGTCTTTCATTAAATTGA+AGG + chr8_1:91613932-91613951 Msa1225930:CDS 20.0%
!!! GAAAGTTTGTATTTTCAAAA+TGG + chr8_1:91614395-91614414 Msa1225930:CDS 20.0%
!! TGATTATCAATACATATTCT+TGG + chr8_1:91614753-91614772 Msa1225930:intron 20.0%
!!! ATATTCTTGGTAATATGTTT+TGG + chr8_1:91614766-91614785 Msa1225930:intron 20.0%
!!! TAATATATGATTTGTTGTTC+TGG + chr8_1:91615383-91615402 Msa1225930:three_prime_UTR 20.0%
!!! TTGTTGTTTTTGATGATGTA+TGG + chr8_1:91612801-91612820 Msa1225930:five_prime_UTR 25.0%
!! GCTGTAATTGATAATCAAAA+TGG + chr8_1:91612854-91612873 Msa1225930:five_prime_UTR 25.0%
! AACAAGGATTCCAATTTAAT+TGG + chr8_1:91613178-91613197 Msa1225930:five_prime_UTR 25.0%
! ACAAGGATTCCAATTTAATT+GGG + chr8_1:91613179-91613198 Msa1225930:five_prime_UTR 25.0%
! CTCTTTAATCCCAATTAAAT+TGG - chr8_1:91613191-91613210 None:intergenic 25.0%
!! AAACATGATCTAAGATAGTA+AGG - chr8_1:91613241-91613260 None:intergenic 25.0%
!!! AGATCATGTTTGTTGTATTT+TGG + chr8_1:91613250-91613269 Msa1225930:five_prime_UTR 25.0%
!! AGAGTTGATTAATAGAAGTT+TGG + chr8_1:91613392-91613411 Msa1225930:five_prime_UTR 25.0%
!! GATGATCTTTGAAAAATTTG+AGG + chr8_1:91613482-91613501 Msa1225930:exon 25.0%
! ATCAAATTGCCTATATTTCT+AGG - chr8_1:91613747-91613766 None:intergenic 25.0%
! TCTTTCATTAAATTGAAGGA+TGG + chr8_1:91613936-91613955 Msa1225930:CDS 25.0%
! ATTAAATTGAAGGATGGTAT+TGG + chr8_1:91613942-91613961 Msa1225930:CDS 25.0%
! AAATCTTGTTGAGTTAAAGT+TGG + chr8_1:91614280-91614299 Msa1225930:CDS 25.0%
!!! ATATATTTTAGCGATTCCAT+TGG - chr8_1:91614329-91614348 None:intergenic 25.0%
!! AGTTTGTATTTTCAAAATGG+AGG + chr8_1:91614398-91614417 Msa1225930:CDS 25.0%
!! GTTTGTATTTTCAAAATGGA+GGG + chr8_1:91614399-91614418 Msa1225930:CDS 25.0%
! GCGAATAGAGAAATTAATTT+AGG - chr8_1:91614891-91614910 None:intergenic 25.0%
!!! ATAATTTGTTGTTAATTGGC+AGG + chr8_1:91615053-91615072 Msa1225930:intron 25.0%
! ATCTTGAATCAATAACTTTG+AGG - chr8_1:91615215-91615234 None:intergenic 25.0%
!!! AGTTATTGATTCAAGATTCT+TGG + chr8_1:91615218-91615237 Msa1225930:three_prime_UTR 25.0%
! TTCATACATGATACATTACA+AGG - chr8_1:91615266-91615285 None:intergenic 25.0%
! CCAGTTATGTATAATTAAAC+TGG - chr8_1:91615446-91615465 None:intergenic 25.0%
! CCAGTTTAATTATACATAAC+TGG + chr8_1:91615443-91615462 Msa1225930:three_prime_UTR 25.0%
! CTTGCTATCAAAAACATTCT+TGG - chr8_1:91612600-91612619 None:intergenic 30.0%
!! CAAGAATGTTTTTGATAGCA+AGG + chr8_1:91612598-91612617 Msa1225930:five_prime_UTR 30.0%
TCTCAATCATACAGTATAGA+AGG + chr8_1:91612662-91612681 Msa1225930:five_prime_UTR 30.0%
CTCAATCATACAGTATAGAA+GGG + chr8_1:91612663-91612682 Msa1225930:five_prime_UTR 30.0%
! ATGTATGGAACATGCATTTT+TGG + chr8_1:91612816-91612835 Msa1225930:five_prime_UTR 30.0%
ACAGATTGTTCTTCAGTTAA+AGG - chr8_1:91612965-91612984 None:intergenic 30.0%
TTTAACTGAAGAACAATCTG+TGG + chr8_1:91612964-91612983 Msa1225930:five_prime_UTR 30.0%
TGTGGAGTTGTTTAATAAGA+AGG + chr8_1:91612982-91613001 Msa1225930:five_prime_UTR 30.0%
TCTACTCTTAGAACTAAACA+AGG + chr8_1:91613162-91613181 Msa1225930:five_prime_UTR 30.0%
ACTCAATTTCATAGTCTTCT+GGG - chr8_1:91613282-91613301 None:intergenic 30.0%
ATCTCTACGATTAACATTGA+TGG + chr8_1:91613420-91613439 Msa1225930:five_prime_UTR 30.0%
! ATTTCTAGGAACAGAATCAA+GGG - chr8_1:91613733-91613752 None:intergenic 30.0%
!! TATTTCTAGGAACAGAATCA+AGG - chr8_1:91613734-91613753 None:intergenic 30.0%
GATTCTGTTCCTAGAAATAT+AGG + chr8_1:91613735-91613754 Msa1225930:CDS 30.0%
TTGAAGCAGTTAAGAGAATT+AGG + chr8_1:91614047-91614066 Msa1225930:CDS 30.0%
! TCAACAAGATTTTGTAGCTT+TGG - chr8_1:91614272-91614291 None:intergenic 30.0%
! CATTGGATCATCTGTTATTT+GGG - chr8_1:91614312-91614331 None:intergenic 30.0%
AAACTAAAGGAACTAACCAT+TGG + chr8_1:91614428-91614447 Msa1225930:CDS 30.0%
ATGCTTTGCACAAATTCATT+TGG - chr8_1:91614605-91614624 None:intergenic 30.0%
GGTTCTATAATCAAACAAAC+TGG + chr8_1:91614787-91614806 Msa1225930:intron 30.0%
TCCAAAAGAAAAGTAAAGTG+AGG - chr8_1:91614913-91614932 None:intergenic 30.0%
! ACTACAACAAAAAGCTTTTG+AGG - chr8_1:91614992-91615011 None:intergenic 30.0%
GTACTGTCTTTCTCTAATAA+TGG + chr8_1:91615161-91615180 Msa1225930:three_prime_UTR 30.0%
GAGGTTTCAAAAAAAAACTG+AGG - chr8_1:91615196-91615215 None:intergenic 30.0%
!!! TGAGAGCTTTTTTTCTTTCA+AGG + chr8_1:91615294-91615313 Msa1225930:three_prime_UTR 30.0%
ATGGAGGAAATTGATGTTGT+GGG + chr8_1:91612468-91612487 Msa1225930:five_prime_UTR 35.0%
GAGTCTAGAGAAAGATTGAT+CGG + chr8_1:91612498-91612517 Msa1225930:five_prime_UTR 35.0%
!! GAATGTTTTTGATAGCAAGG+AGG + chr8_1:91612601-91612620 Msa1225930:five_prime_UTR 35.0%
TATACTACGGAAATGGATAG+AGG + chr8_1:91612731-91612750 Msa1225930:five_prime_UTR 35.0%
ATACTACGGAAATGGATAGA+GGG + chr8_1:91612732-91612751 Msa1225930:five_prime_UTR 35.0%
! GATATATCAATGAGCGCTTT+TGG - chr8_1:91613031-91613050 None:intergenic 35.0%
TAAAGATAAAAGACCTCCGA+TGG - chr8_1:91613101-91613120 None:intergenic 35.0%
GAGATAAAAATGCAATCGAG+TGG + chr8_1:91613122-91613141 Msa1225930:five_prime_UTR 35.0%
GACTCAATTTCATAGTCTTC+TGG - chr8_1:91613283-91613302 None:intergenic 35.0%
TAAGATACTACTTCGTCAGT+GGG + chr8_1:91613303-91613322 Msa1225930:five_prime_UTR 35.0%
AAGGGTTTGTGAAAAGGAAA+AGG + chr8_1:91613332-91613351 Msa1225930:five_prime_UTR 35.0%
AGGGTTTGTGAAAAGGAAAA+GGG + chr8_1:91613333-91613352 Msa1225930:five_prime_UTR 35.0%
GAAGACATTAGAAGAAGTTG+CGG + chr8_1:91613356-91613375 Msa1225930:five_prime_UTR 35.0%
ACTGACTGTCTTCACTAATA+TGG - chr8_1:91613524-91613543 None:intergenic 35.0%
!! TTGTTTCTATTGATAGACGG+CGG - chr8_1:91613566-91613585 None:intergenic 35.0%
GTATTGAAAGCTCACATGTT+CGG + chr8_1:91613607-91613626 Msa1225930:CDS 35.0%
TATTGAAAGCTCACATGTTC+GGG + chr8_1:91613608-91613627 Msa1225930:CDS 35.0%
AAAAGCCTTCCAAAATCCAT+TGG + chr8_1:91613804-91613823 Msa1225930:CDS 35.0%
! TAATCTCTTTTGGCATCTCA+TGG - chr8_1:91613875-91613894 None:intergenic 35.0%
!!! CTAAGCTTGCTAATCTCTTT+TGG - chr8_1:91613885-91613904 None:intergenic 35.0%
AGAAGTAGTAGAACTATTCC+AGG + chr8_1:91614013-91614032 Msa1225930:CDS 35.0%
TTCGAGATCTTAGACTGTAT+GGG + chr8_1:91614219-91614238 Msa1225930:CDS 35.0%
CCATTGGATCATCTGTTATT+TGG - chr8_1:91614313-91614332 None:intergenic 35.0%
CCAAATAACAGATGATCCAA+TGG + chr8_1:91614310-91614329 Msa1225930:CDS 35.0%
!! CACTTGTTGTCACTCTATAT+CGG + chr8_1:91614353-91614372 Msa1225930:CDS 35.0%
AACTTTCACCTTCATAACCA+TGG - chr8_1:91614382-91614401 None:intergenic 35.0%
CAAGGAAGATGAATTTCCAA+TGG - chr8_1:91614447-91614466 None:intergenic 35.0%
TGTTCCATCATCATTGACAA+AGG + chr8_1:91614467-91614486 Msa1225930:CDS 35.0%
TGATCCTTTGTCAATGATGA+TGG - chr8_1:91614474-91614493 None:intergenic 35.0%
! AGCTTCAAGTTCTCAGTATT+TGG + chr8_1:91614573-91614592 Msa1225930:CDS 35.0%
! GCTTCAAGTTCTCAGTATTT+GGG + chr8_1:91614574-91614593 Msa1225930:CDS 35.0%
GCAGGATAAAACTCTACAAA+GGG - chr8_1:91614671-91614690 None:intergenic 35.0%
AGCAGGATAAAACTCTACAA+AGG - chr8_1:91614672-91614691 None:intergenic 35.0%
!! TTTGTAGAGTTTTATCCTGC+TGG + chr8_1:91614671-91614690 Msa1225930:CDS 35.0%
TACTTGAGGACAAAGAACTA+AGG + chr8_1:91614710-91614729 Msa1225930:CDS 35.0%
!! GCCTCACTTTACTTTTCTTT+TGG + chr8_1:91614909-91614928 Msa1225930:intron 35.0%
!! ATTTGTTGTTAATTGGCAGG+TGG + chr8_1:91615056-91615075 Msa1225930:intron 35.0%
TAAATGGTATGACCCTCGAT+TGG + chr8_1:91612440-91612459 Msa1225930:five_prime_UTR 40.0%
CATGGAGGAAATTGATGTTG+TGG + chr8_1:91612467-91612486 Msa1225930:five_prime_UTR 40.0%
! TTTGATAGCAAGGAGGTTAG+TGG + chr8_1:91612608-91612627 Msa1225930:five_prime_UTR 40.0%
GACACTTTGATTGTCGTGTA+TGG + chr8_1:91612630-91612649 Msa1225930:five_prime_UTR 40.0%
ACAGTATAGAAGGGTTGTTG+AGG + chr8_1:91612672-91612691 Msa1225930:five_prime_UTR 40.0%
CAGTATAGAAGGGTTGTTGA+GGG + chr8_1:91612673-91612692 Msa1225930:five_prime_UTR 40.0%
GTCTTTGCCTCAGTATACTA+CGG + chr8_1:91612718-91612737 Msa1225930:five_prime_UTR 40.0%
TCCATTTCCGTAGTATACTG+AGG - chr8_1:91612728-91612747 None:intergenic 40.0%
TAGAGGGTCATTAATCGATG+AGG + chr8_1:91612748-91612767 Msa1225930:five_prime_UTR 40.0%
GAAACTACTTGCAGCAAAAG+AGG + chr8_1:91612774-91612793 Msa1225930:five_prime_UTR 40.0%
CAACTCATGCACTTTGACAA+AGG - chr8_1:91612939-91612958 None:intergenic 40.0%
AAAATGCAATCGAGTGGAAG+AGG + chr8_1:91613128-91613147 Msa1225930:five_prime_UTR 40.0%
CTAAGATACTACTTCGTCAG+TGG + chr8_1:91613302-91613321 Msa1225930:five_prime_UTR 40.0%
TAGCTGAAGGGTTTGTGAAA+AGG + chr8_1:91613326-91613345 Msa1225930:five_prime_UTR 40.0%
GGGTTTGTGAAAAGGAAAAG+GGG + chr8_1:91613334-91613353 Msa1225930:five_prime_UTR 40.0%
ACGGCGGTATTGTTTACTTA+AGG - chr8_1:91613550-91613569 None:intergenic 40.0%
! CATCCAAAGTCTCTAGGTTT+TGG - chr8_1:91613836-91613855 None:intergenic 40.0%
CTCTTACATCCAAAGTCTCT+AGG - chr8_1:91613842-91613861 None:intergenic 40.0%
CTAGAGACTTTGGATGTAAG+AGG + chr8_1:91613840-91613859 Msa1225930:CDS 40.0%
AGAAAGCTACTACATCTTCG+AGG + chr8_1:91613903-91613922 Msa1225930:CDS 40.0%
!!! GCTTCAACTTTTTTAGCTCC+TGG - chr8_1:91614034-91614053 None:intergenic 40.0%
GCAGTTAAGAGAATTAGGCT+TGG + chr8_1:91614052-91614071 Msa1225930:CDS 40.0%
ACTCAATGAGATGCAACACT+TGG + chr8_1:91614115-91614134 Msa1225930:CDS 40.0%
! AGTCTAAGATCTCGAAGCAT+TGG - chr8_1:91614215-91614234 None:intergenic 40.0%
CTTCGAGATCTTAGACTGTA+TGG + chr8_1:91614218-91614237 Msa1225930:CDS 40.0%
TTTCACCTTCATAACCATGG+TGG - chr8_1:91614379-91614398 None:intergenic 40.0%
TGGAGGGTTTCACAAACTAA+AGG + chr8_1:91614415-91614434 Msa1225930:CDS 40.0%
!! GTCAATGATGATGGAACACA+AGG - chr8_1:91614465-91614484 None:intergenic 40.0%
CTCTTAGAAAGCTCGAGTTA+TGG + chr8_1:91614501-91614520 Msa1225930:CDS 40.0%
AAACTCAAGACTGTACCTAC+CGG + chr8_1:91614533-91614552 Msa1225930:CDS 40.0%
CTTGAAGCTTCTCTAAGTGT+TGG - chr8_1:91614562-91614581 None:intergenic 40.0%
!! CAAAGCATTGCTCCAAATGA+AGG + chr8_1:91614617-91614636 Msa1225930:CDS 40.0%
! CAGTGCTCTTTTCCTTCATT+TGG - chr8_1:91614632-91614651 None:intergenic 40.0%
! CAAATGAAGGAAAAGAGCAC+TGG + chr8_1:91614630-91614649 Msa1225930:CDS 40.0%
GCAAATCTGTTCGATACTTG+AGG + chr8_1:91614696-91614715 Msa1225930:CDS 40.0%
GAGGACAAAGAACTAAGGTA+TGG + chr8_1:91614715-91614734 Msa1225930:intron 40.0%
TATAGTTCAGTCGACTGTAG+AGG + chr8_1:91614828-91614847 Msa1225930:intron 40.0%
ATTTCTCTGCTCAGTGTGAA+AGG - chr8_1:91615015-91615034 None:intergenic 40.0%
AACGGTTGTACAAGTACACT+TGG - chr8_1:91615108-91615127 None:intergenic 40.0%
CTAGAGAAAGATTGATCGGC+TGG + chr8_1:91612502-91612521 Msa1225930:five_prime_UTR 45.0%
AGAAAGATTGATCGGCTGGT+TGG + chr8_1:91612506-91612525 Msa1225930:five_prime_UTR 45.0%
! ATTGATCGGCTGGTTGGAAA+AGG + chr8_1:91612512-91612531 Msa1225930:five_prime_UTR 45.0%
GCCTCAGTATACTACGGAAA+TGG + chr8_1:91612724-91612743 Msa1225930:five_prime_UTR 45.0%
CCACTAGCAATTGTTGCCAT+CGG + chr8_1:91613082-91613101 Msa1225930:five_prime_UTR 45.0%
!! GCATGCCAATGGATTTTGGA+AGG - chr8_1:91613812-91613831 None:intergenic 45.0%
!! TGGAGCATGCCAATGGATTT+TGG - chr8_1:91613816-91613835 None:intergenic 45.0%
!!! TAGGTTTTGGAGCATGCCAA+TGG - chr8_1:91613823-91613842 None:intergenic 45.0%
GCTCCAAAACCTAGAGACTT+TGG + chr8_1:91613830-91613849 Msa1225930:CDS 45.0%
GTTCGTCTTAGAAGAGAGCA+TGG + chr8_1:91614074-91614093 Msa1225930:CDS 45.0%
!! CTAAGATCTCGAAGCATTGG+TGG - chr8_1:91614212-91614231 None:intergenic 45.0%
!! TGTAGCTTTGGAATCCACTC+TGG - chr8_1:91614260-91614279 None:intergenic 45.0%
GGTAGGTACAGTCTTGAGTT+TGG - chr8_1:91614534-91614553 None:intergenic 45.0%
!! CTTCTCTAAGTGTTGGATGC+CGG - chr8_1:91614555-91614574 None:intergenic 45.0%
! TACAAAGGGCACATGCTGAA+TGG - chr8_1:91614657-91614676 None:intergenic 45.0%
!! GTAGAGTTTTATCCTGCTGG+TGG + chr8_1:91614674-91614693 Msa1225930:CDS 45.0%
TCAGTCGACTGTAGAGGATT+CGG + chr8_1:91614834-91614853 Msa1225930:intron 45.0%
AGTACACTTGGAGCAATCAC+TGG - chr8_1:91615096-91615115 None:intergenic 45.0%
GTGTACTTGTACAACCGTTG+CGG + chr8_1:91615109-91615128 Msa1225930:three_prime_UTR 45.0%
! TGACCCTCGATTGGTTCACA+TGG + chr8_1:91612449-91612468 Msa1225930:five_prime_UTR 50.0%
CCTCCATGTGAACCAATCGA+GGG - chr8_1:91612455-91612474 None:intergenic 50.0%
TCCTCCATGTGAACCAATCG+AGG - chr8_1:91612456-91612475 None:intergenic 50.0%
! TGTTGTCTCTGTGGTGGGAA+TGG + chr8_1:91612550-91612569 Msa1225930:five_prime_UTR 50.0%
! GTTGTCTCTGTGGTGGGAAT+GGG + chr8_1:91612551-91612570 Msa1225930:five_prime_UTR 50.0%
CCGATGGCAACAATTGCTAG+TGG - chr8_1:91613085-91613104 None:intergenic 50.0%
CTAGCAATTGTTGCCATCGG+AGG + chr8_1:91613085-91613104 Msa1225930:five_prime_UTR 50.0%
TTCGTCAGTGGGTAGCTGAA+GGG + chr8_1:91613314-91613333 Msa1225930:five_prime_UTR 50.0%
AGTTAGAGAAGCTGCCAGAG+TGG + chr8_1:91614243-91614262 Msa1225930:CDS 50.0%
TCACTCTATATCGGCCACCA+TGG + chr8_1:91614362-91614381 Msa1225930:CDS 50.0%
ATCGGCCACCATGGTTATGA+AGG + chr8_1:91614371-91614390 Msa1225930:CDS 50.0%
!! TCTAAGTGTTGGATGCCGGT+AGG - chr8_1:91614551-91614570 None:intergenic 50.0%
TTGGAACAGACATGCCGCAA+CGG - chr8_1:91615126-91615145 None:intergenic 50.0%
! CCCTCGATTGGTTCACATGG+AGG + chr8_1:91612452-91612471 Msa1225930:five_prime_UTR 55.0%
AGAGCGCACTGTTGTCTCTG+TGG + chr8_1:91612541-91612560 Msa1225930:five_prime_UTR 55.0%
CGCACTGTTGTCTCTGTGGT+GGG + chr8_1:91612545-91612564 Msa1225930:five_prime_UTR 55.0%
! TCTCTGTGGTGGGAATGGGA+GGG + chr8_1:91612555-91612574 Msa1225930:five_prime_UTR 55.0%
CTTCGTCAGTGGGTAGCTGA+AGG + chr8_1:91613313-91613332 Msa1225930:five_prime_UTR 55.0%
!! TCGGGCGCTGCTTGTTTTCA+CGG + chr8_1:91613626-91613645 Msa1225930:CDS 55.0%
CGAACAGATTTGCCACCAGC+AGG - chr8_1:91614689-91614708 None:intergenic 55.0%
GTTAATTGGCAGGTGGTGCC+TGG + chr8_1:91615063-91615082 Msa1225930:intron 55.0%
GCAATCACTGGAAGCCAACC+AGG - chr8_1:91615084-91615103 None:intergenic 55.0%
GCGCACTGTTGTCTCTGTGG+TGG + chr8_1:91612544-91612563 Msa1225930:five_prime_UTR 60.0%
! GTCTCTGTGGTGGGAATGGG+AGG + chr8_1:91612554-91612573 Msa1225930:five_prime_UTR 60.0%
ATTGGCAGGTGGTGCCTGGT+TGG + chr8_1:91615067-91615086 Msa1225930:intron 60.0%
! TGTGGTGGGAATGGGAGGGC+AGG + chr8_1:91612559-91612578 Msa1225930:five_prime_UTR 65.0%
! GTGGTGGGAATGGGAGGGCA+GGG + chr8_1:91612560-91612579 Msa1225930:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 91612433 91615494 91612433 ID=Msa1225930;Name=Msa1225930
chr8_1 mRNA 91612433 91615494 91612433 ID=Msa1225930-mRNA-1;Parent=Msa1225930;Name=Msa1225930-mRNA-1;_AED=0.00;_eAED=0.00;_QI=1050|0|0.5|1|0|0.5|2|420|415
chr8_1 exon 91612433 91614730 91612433 ID=Msa1225930-mRNA-1:exon:33393;Parent=Msa1225930-mRNA-1
chr8_1 exon 91615075 91615494 91615075 ID=Msa1225930-mRNA-1:exon:33394;Parent=Msa1225930-mRNA-1
chr8_1 five_prime_UTR 91612433 91613482 91612433 ID=Msa1225930-mRNA-1:five_prime_utr;Parent=Msa1225930-mRNA-1
chr8_1 CDS 91613483 91614730 91613483 ID=Msa1225930-mRNA-1:cds;Parent=Msa1225930-mRNA-1
chr8_1 three_prime_UTR 91615075 91615494 91615075 ID=Msa1225930-mRNA-1:three_prime_utr;Parent=Msa1225930-mRNA-1
Gene Sequence

>Msa1225930

ATGATCTTTGAAAAATTTGAGGATTTAAGTTTTTGCTGCCATATTAGTGAAGACAGTCAGTCATCCTTAAGTAAACAATACCGCCGTCTATCAATAGAAACAAATTCTAATGATTTAATGGCAAGTATTGAAAGCTCACATGTTCGGGCGCTGCTTGTTTTCACGGATAAAGAAATAACTGAAGACTTTGCAAATAAAATCACTACAAAATTCAAGTTGTTGAAAGTGCTTGATTTTAAAGATACATCCCTTGATTCTGTTCCTAGAAATATAGGCAATTTGATTCACTTGAAGTACTTAAGCTTTAGAAACACAGATATAAAAAGCCTTCCAAAATCCATTGGCATGCTCCAAAACCTAGAGACTTTGGATGTAAGAGGTACAGATATCCATGAGATGCCAAAAGAGATTAGCAAGCTTAGAAAGCTACTACATCTTCGAGGTTATACAATGTCTTTCATTAAATTGAAGGATGGTATTGGAGACATGACATCTCTACAATCATTGCGATATGTTTTATTAGATGCAGAAGAAGTAGTAGAACTATTCCAGGAGCTAAAAAAGTTGAAGCAGTTAAGAGAATTAGGCTTGGTTCGTCTTAGAAGAGAGCATGGAAGTGTTCTATCTTCTTTACTCAATGAGATGCAACACTTGGAGAAACTACATATTCGATCACAATCAACAAACACAAATGAAGTCAATATTGATTTACATTTGATTTCATGTCCACCAATGCTTCGAGATCTTAGACTGTATGGGAAGTTAGAGAAGCTGCCAGAGTGGATTCCAAAGCTACAAAATCTTGTTGAGTTAAAGTTGGAATTCACCCAAATAACAGATGATCCAATGGAATCGCTAAAATATATGCAACACTTGTTGTCACTCTATATCGGCCACCATGGTTATGAAGGTGAAAGTTTGTATTTTCAAAATGGAGGGTTTCACAAACTAAAGGAACTAACCATTGGAAATTCATCTTCCTTGTGTTCCATCATCATTGACAAAGGATCATTGTGTTCTCTTAGAAAGCTCGAGTTATGGCGTAACACCAAACTCAAGACTGTACCTACCGGCATCCAACACTTAGAGAAGCTTCAAGTTCTCAGTATTTGGGATATGCCAAATGAATTTGTGCAAAGCATTGCTCCAAATGAAGGAAAAGAGCACTGGACCATTCAGCATGTGCCCTTTGTAGAGTTTTATCCTGCTGGTGGCAAATCTGTTCGATACTTGAGGACAAAGAACTAA
Protein sequence

>Msa1225930

MIFEKFEDLSFCCHISEDSQSSLSKQYRRLSIETNSNDLMASIESSHVRALLVFTDKEITEDFANKITTKFKLLKVLDFKDTSLDSVPRNIGNLIHLKYLSFRNTDIKSLPKSIGMLQNLETLDVRGTDIHEMPKEISKLRKLLHLRGYTMSFIKLKDGIGDMTSLQSLRYVLLDAEEVVELFQELKKLKQLRELGLVRLRREHGSVLSSLLNEMQHLEKLHIRSQSTNTNEVNIDLHLISCPPMLRDLRLYGKLEKLPEWIPKLQNLVELKLEFTQITDDPMESLKYMQHLLSLYIGHHGYEGESLYFQNGGFHKLKELTIGNSSSLCSIIIDKGSLCSLRKLELWRNTKLKTVPTGIQHLEKLQVLSIWDMPNEFVQSIAPNEGKEHWTIQHVPFVEFYPAGGKSVRYLRTKN*