AlfalfaGEDB | Msa1225970

M. sativa cultivar zhongmu-4 / Msa1225970

KEGG: K13457
GO: GO:0000166 GO:0002376 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0008219 GO:0009626 GO:0009987 GO:0012501 GO:0016020 GO:0019897 GO:0019898 GO:0023052 GO:0033554 GO:0034050 GO:0036094 GO:0044425 GO:0044459 GO:0044464 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944 GO:0097159 GO:1901265 GO:1901363
PFAM: PF18052.4

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1225970	sp\|Q39214\|RPM1_ARATH	30.720	931	598	17	1	903	1	912	6.44e-129	414

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1225970	A0A072UX30	93.696	920	55	2	1	917	1	920	0.0	1753

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0454880	Msa1225970	0.916717	1.272385e-85	-8.615850e-47
Msa0669990	Msa1225970	0.805267	1.449554e-49	-8.615850e-47
Msa0832490	Msa1225970	0.949702	7.442253e-108	-8.615850e-47
Msa1225930	Msa1225970	0.812463	4.186347e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa1225970	MtrunA17_Chr3g0101321	93.696	920	55	2	1	917	1	920	1753
Msa1225970	MtrunA17_Chr3g0112511	62.743	926	321	9	1	915	1	913	1095
Msa1225970	MtrunA17_Chr3g0101331	61.632	907	315	13	1	876	1	905	1036
Msa1225970	MtrunA17_Chr1g0189351	60.066	904	328	13	1	876	1	899	1013
Msa1225970	MtrunA17_Chr3g0100831	57.328	928	369	12	1	913	1	916	999
Msa1225970	MtrunA17_Chr3g0100961	57.605	927	361	13	1	913	1	909	993
Msa1225970	MtrunA17_Chr3g0100801	57.901	829	279	10	4	820	1	771	891
Msa1225970	MtrunA17_Chr3g0101271	52.620	897	390	10	21	901	8	885	868
Msa1225970	MtrunA17_Chr3g0101241	52.620	897	390	10	21	901	8	885	867
Msa1225970	MtrunA17_Chr3g0080601	50.432	926	427	12	1	900	1	920	865

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1225970	AT3G07040.1	30.720	931	598	17	1	903	1	912	6.55e-130	414
Msa1225970	AT5G48620.6	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G48620.1	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G48620.4	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G48620.2	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G48620.3	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G48620.5	29.756	941	584	28	1	902	1	903	2.08e-100	335
Msa1225970	AT5G35450.2	29.355	930	593	25	1	903	1	893	8.21e-97	325
Msa1225970	AT5G35450.1	29.355	930	593	25	1	903	1	893	8.21e-97	325
Msa1225970	AT5G43470.3	30.000	940	583	29	1	902	1	903	2.47e-96	323

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Find 183 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCCTTCCAAAATCCAT+TGG	0.555015	8_1:+91652681	Msa1225970:CDS
AAAATGCAATCGAGTGGAAG+AGG	0.536610	8_1:+91652004	Msa1225970:CDS
AAACATGATCTAAGATAGTA+AGG	0.449915	8_1:-91652114	None:intergenic
AAACTAAAGGAACTAACCAT+TGG	0.519283	8_1:+91653305	Msa1225970:CDS
AAACTCAAGACTGTACCTAC+CGG	0.622920	8_1:+91653410	Msa1225970:CDS
AAAGAAGCCAATTTGCTTAG+AGG	0.336733	8_1:+91650908	Msa1225970:CDS
AAAGACATTAGAAGAAGTTG+CGG	0.654596	8_1:+91652233	Msa1225970:CDS
AAATCTTGTTGAGTTAAAGT+TGG	0.439534	8_1:+91653157	Msa1225970:CDS
AACAAATTCTAATGATTTAA+TGG	0.158680	8_1:+91652458	Msa1225970:CDS
AACAAGGATTCCAATTTAAT+TGG	0.110890	8_1:+91652054	Msa1225970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAATTTATTTACTCCCAC+TGG	+	chr8_1:91650738-91650757	Msa1225970:five_prime_UTR	30.0%
AAGGAAAGAAGTGAAAGATA+TGG	+	chr8_1:91651247-91651266	Msa1225970:CDS	30.0%
TTGATTCTTGATGTTGGATT+TGG	-	chr8_1:91651276-91651295	None:intergenic	30.0%
GTGGAATACAAAATGCTAAA+TGG	+	chr8_1:91651296-91651315	Msa1225970:CDS	30.0%
CTTGCTTTCAAAAACATTCT+TGG	-	chr8_1:91651473-91651492	None:intergenic	30.0%
TCTCAATCATACAGTATAGA+AGG	+	chr8_1:91651535-91651554	Msa1225970:CDS	30.0%
CTCAATCATACAGTATAGAA+GGG	+	chr8_1:91651536-91651555	Msa1225970:CDS	30.0%
ATTACTATGGAAATGGATAG+AGG	+	chr8_1:91651607-91651626	Msa1225970:CDS	30.0%
TTACTATGGAAATGGATAGA+GGG	+	chr8_1:91651608-91651627	Msa1225970:CDS	30.0%
ACAGATTGTTCTTCAGTTAA+AGG	-	chr8_1:91651841-91651860	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	91650678	91654386	91650678	ID=Msa1225970;Name=Msa1225970
chr8_1	mRNA	91650678	91654386	91650678	ID=Msa1225970-mRNA-1;Parent=Msa1225970;Name=Msa1225970-mRNA-1;_AED=0.28;_eAED=0.28;_QI=176\|0\|0.5\|1\|0\|1\|2\|433\|917
chr8_1	exon	91650678	91653607	91650678	ID=Msa1225970-mRNA-1:exon:33395;Parent=Msa1225970-mRNA-1
chr8_1	exon	91653954	91654386	91653954	ID=Msa1225970-mRNA-1:exon:33396;Parent=Msa1225970-mRNA-1
chr8_1	five_prime_UTR	91650678	91650853	91650678	ID=Msa1225970-mRNA-1:five_prime_utr;Parent=Msa1225970-mRNA-1
chr8_1	CDS	91650854	91653607	91650854	ID=Msa1225970-mRNA-1:cds;Parent=Msa1225970-mRNA-1
chr8_1	three_prime_UTR	91653954	91654386	91653954	ID=Msa1225970-mRNA-1:three_prime_utr;Parent=Msa1225970-mRNA-1

Gene Sequence

>Msa1225970

ATGGCTGAAATGGCTGTGAGTTTGGCTATTCAGCAACTGCTTCCACTGTTAAGGAAAGAAGCCAATTTGCTTAGAGGTATCCACAAGGAATTTGCATACATCAAAGATGAACTTGAAAGCATCCAAGCATTCCTTAAGGACGCCGAAAAAAGAGCTGCAGTTGAAGGAGACAACGTCCGTGAAGGGGTCAAAATATGGGTGAAGCAGGTGATAGGAGTAGCTTTTCGTATAGAAGATATCATAGATGAATATATGATCTATGAACCACGAAAACCTCATCATCCCAAATATCCAGATTCACTCCATAAGATTGTTCAATTGATCAAAACTTTGATCCCTCGCCATCGAATTGGGTCAGAGATTCGAGAAATTAAGTCATCTGTTTGTATGATCAAGGAAAGAAGTGAAAGATATGGCTTCCAAATCCAACATCAAGAATCAAGTGGAATACAAAATGCTAAATGGTATGACCCTCGATTGGTTCACATGGAGGAAATTGATGTTGTGGGATTTCAAGAGTCTAGAGAAAGATTGATCGGCTGGTTGGTAAAAGGAAGAGTAGAGCGCACTGTCGTCTCTGTGGTGGGAATGGGAGGGCAGGGTAAAACCACTCTTGCCAAGAATGTTTTTGAAAGCAAGGAGGTTAGTGGACACTTTGATTGTCGTGTATGGATCACAGTGTCTCAATCATACAGTATAGAAGGGTTGTTGAGGGACATGTTGCGCAAGTTTCACAAGTCTTTGTCTCATGATATTACTATGGAAATGGATAGAGGGTCATTAATCGATGAGGTAAGAAACTACTTGCAGCAAAAGAGGTACGTTGTTGTTTTTGATGATGTATGGAACATGCATTTTTGGAATGAGATTGAATCTGCTGTAATTGATAATAAAAATGGAAGTAAGATATTTATAACAACAAGGAATATAGATGTTGCACTTTCTTGTAAGAGATCTTCGTTTGTCAAAGTGCATGAGTTGCAACCTTTAACTGAAGAACAATCTGTGGAGTTGTTTAATAAGAAGGCATTTCGATCTGACGAATGTTGTCCAAAAGAGCTCATTGATATATCTTGTGATATTGTTAAAAAATGCAAAGGTTTACCACTAGCAATTGTTGCCATCGGAGGTCTTTTATCTTTAAGAGATAAAAATGCAATCGAGTGGAAGAGGTTTTGTGAAAATCTACTCTTAGAGCTAAACAAGGATTCCAATTTAATTGGGATTAAAGAGATTTTAAGATTGAGTTATGATGATTTGCCTTACTATCTTAGATCATGTTTGTTGTATTTTGGATTATACCCAGAAGACTATGAAATTGAGTCTAAGATACTACTTCGTCAGTGGGTAGCTGAAGGGTTTGTGAAAGAGGAAAGGGGAAAGACATTAGAAGAAGTTGCGGAAACTTATTTATCAGAGTTGATTAATAGAAGTTTGGTGCAAATCTCTATGATTAACATTGATGGAAAAGTTAAAAGTTGTCATGTTCATGATCTTTTACGTGTGATGATCTTTGAAAAATTTGAGGATTTAAGTTTTTGTTGCCATATTAGTGAAGACAGTCAGTCATCCTTAAGTAAACAATACCGCCGTCTATCAATAGAAACAAATTCTAATGATTTAATGGCAAGTATTGAAAGCTCACATGTTCGGGCGCTGCTTGTTTTCACGGATAAAGAGATAACTGAAGACTTTGCAAATAAAATCACTACAAAATTCAAGTTGTTGAAAGTGCTTGATTTTAAAGATACATCCCTTGATTCTGTTCCTAGAAATATAGGTAATTTGATTCACTTGAAGTACTTAAGCTTTAGAAACACAGATATAAAAAGCCTTCCAAAATCCATTGGCATGCTCCAAAACCTAGAGACTTTGGATGTAAGAGGTACAGATATCCATGAGATGCCAAAAGAGATTAGCAAGCTTAGAAAGCTACTACATCTTCGAGGTTATACAATGTCTTTCATTAAATTGAAGGATGGTATTGGAGACATGACATCTCTACAATCATTGCGATATGTTTTATTAGATGCAGAAGAAGTAGTAGAGCTATTCCAGGAGCTAAAAAAGTTGAAGCAGTTAAGAGAATTAGGCTTGGTTCGTCTTAGAAGAGAGCATGGAAGTGTTCTATCTTCTTTACTCAATGAGATGCAACACTTGGAGAAACTACATATTCGATCACAATCAACAAACACAAATGAAGTCAATATTGATTTACATTTGATTTCATGTCCACCAATGCTTCGAGATCTTAGACTGTATGGGAAGTTAGAGAAGCTGCCAGAGTGGATTCCAAAGCTACAAAATCTTGTTGAGTTAAAGTTGGAATTCACCCAAATAACAGATGATCCAATGGAATCACTAAAATATATGCAACACTTGCTGTCACTCTATATCGGCCACCATGGTTATGAAGGTGAAAGTTTGTATTTTCAAAATGGAGGGTTTCACAAACTAAAGGAACTAACCATTGGAAATTCATCTTCCTTGTGTTCCATCATCATTGACAAAGGATCATTGTGTTCTCTTAGAAAGCTCGAGTTATGGCGTAACACCAAACTCAAGACTGTACCTACCGGCATCCAACACTTAGAGAAGCTTCAAGTTCTCAGTATTTGGGATATGCCAAATGAATTTGTGCAAAGCATTGCTCCAAATGAAGGAAAAGAGCACTGGACCATTCAGCATGTGCCCTTTGTAGAGTTTTATCCTGCTGGCGGCAAATCTGTTCGATACTTAAGGACAAAGAACTAA

Protein sequence

>Msa1225970

MAEMAVSLAIQQLLPLLRKEANLLRGIHKEFAYIKDELESIQAFLKDAEKRAAVEGDNVREGVKIWVKQVIGVAFRIEDIIDEYMIYEPRKPHHPKYPDSLHKIVQLIKTLIPRHRIGSEIREIKSSVCMIKERSERYGFQIQHQESSGIQNAKWYDPRLVHMEEIDVVGFQESRERLIGWLVKGRVERTVVSVVGMGGQGKTTLAKNVFESKEVSGHFDCRVWITVSQSYSIEGLLRDMLRKFHKSLSHDITMEMDRGSLIDEVRNYLQQKRYVVVFDDVWNMHFWNEIESAVIDNKNGSKIFITTRNIDVALSCKRSSFVKVHELQPLTEEQSVELFNKKAFRSDECCPKELIDISCDIVKKCKGLPLAIVAIGGLLSLRDKNAIEWKRFCENLLLELNKDSNLIGIKEILRLSYDDLPYYLRSCLLYFGLYPEDYEIESKILLRQWVAEGFVKEERGKTLEEVAETYLSELINRSLVQISMINIDGKVKSCHVHDLLRVMIFEKFEDLSFCCHISEDSQSSLSKQYRRLSIETNSNDLMASIESSHVRALLVFTDKEITEDFANKITTKFKLLKVLDFKDTSLDSVPRNIGNLIHLKYLSFRNTDIKSLPKSIGMLQNLETLDVRGTDIHEMPKEISKLRKLLHLRGYTMSFIKLKDGIGDMTSLQSLRYVLLDAEEVVELFQELKKLKQLRELGLVRLRREHGSVLSSLLNEMQHLEKLHIRSQSTNTNEVNIDLHLISCPPMLRDLRLYGKLEKLPEWIPKLQNLVELKLEFTQITDDPMESLKYMQHLLSLYIGHHGYEGESLYFQNGGFHKLKELTIGNSSSLCSIIIDKGSLCSLRKLELWRNTKLKTVPTGIQHLEKLQVLSIWDMPNEFVQSIAPNEGKEHWTIQHVPFVEFYPAGGKSVRYLRTKN*