AlfalfaGEDB | Msa1227180

M. sativa cultivar zhongmu-4 / Msa1227180

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004518 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043207 GO:0043565 GO:0044237 GO:0044238 GO:0046483 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0098542 GO:1901360 GO:1901363
PFAM: PF02577.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227180	XP_024631172.1	90.751	346	11	1	1	346	1	325	0.0	645

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227180	sp\|Q9FWS6\|BBD1_ARATH	61.272	346	109	4	1	344	1	323	5.74e-153	435

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003420	Msa1227180	0.876897	9.688115e-69	-8.615850e-47
Msa0050880	Msa1227180	0.836918	7.060384e-57	-8.615850e-47
Msa0617560	Msa1227180	-0.813537	2.433952e-51	-8.615850e-47
Msa1227060	Msa1227180	0.836544	8.794424e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227180	MtrunA17_Chr1g0155531	90.751	346	11	1	1	346	1	325	0.0	645
Msa1227180	MtrunA17_Chr3g0141831	66.476	349	84	3	1	346	4	322	6.95e-159	447
Msa1227180	MtrunA17_Chr5g0406211	33.333	174	109	4	134	305	141	309	3.92e-23	98.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1227180	AT1G75380.3	61.272	346	109	4	1	344	1	323	5.84e-154	435
Msa1227180	AT1G75380.1	61.272	346	109	4	1	344	1	323	5.84e-154	435
Msa1227180	AT1G75380.4	61.272	346	109	4	1	344	1	323	5.84e-154	435
Msa1227180	AT1G75380.2	61.272	346	109	4	1	344	1	323	5.84e-154	435
Msa1227180	AT1G19660.2	58.333	348	120	3	1	344	1	327	3.74e-146	416
Msa1227180	AT1G19660.1	58.333	348	120	3	1	344	1	327	3.74e-146	416
Msa1227180	AT1G19660.3	63.754	309	108	2	1	305	1	309	7.19e-144	410
Msa1227180	AT5G66050.2	34.314	204	105	6	133	334	49	225	8.60e-23	95.9
Msa1227180	AT5G66050.1	34.314	204	105	6	133	334	143	319	2.98e-22	96.3
Msa1227180	AT5G66050.3	34.314	204	105	6	133	334	143	319	3.44e-22	95.9

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Find 90 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGACATACAAACAATTAT+AGG	0.305774	8_2:-2265342	None:intergenic
AACTTGACCCGCTGTAAATC+CGG	0.439350	8_2:+2263789	None:intergenic
AAGGTTGTAACAGAAGAAGC+TGG	0.619996	8_2:-2263891	None:intergenic
AAGTACTTGGTGTCCAGTGA+TGG	0.563535	8_2:+2266302	Msa1227180:CDS
AAGTACTTGTTGACTTGTAT+TGG	0.406137	8_2:-2266287	None:intergenic
AAGTGACATTGCAGGCCAAA+TGG	0.514058	8_2:+2266497	Msa1227180:intron
AATAATCGACCGTCAAAACT+AGG	0.444954	8_2:-2266365	None:intergenic
AATACAAGTCAACAAGTACT+TGG	0.598418	8_2:+2266289	Msa1227180:CDS
AATTACTCTCATTCCATCAC+TGG	0.485713	8_2:-2266315	None:intergenic
AATTCATCAGTTTCAGCACA+AGG	0.582528	8_2:-2266524	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGATAGTATAAGTGCATGT+AGG	+	chr8_2:2264503-2264522	Msa1227180:intron	30.0%
AGATAGTATAAGTGCATGTA+GGG	+	chr8_2:2264504-2264523	Msa1227180:intron	30.0%
TTGTTAAGCTTTCAACCAAT+TGG	+	chr8_2:2264542-2264561	Msa1227180:intron	30.0%
CTAACACATCTAACATTCAT+TGG	-	chr8_2:2264833-2264852	None:intergenic	30.0%
TTCATAATCAAAATGAGGGA+TGG	+	chr8_2:2265210-2265229	Msa1227180:CDS	30.0%
GACATTCCAGAAAATTACAA+TGG	-	chr8_2:2265442-2265461	None:intergenic	30.0%
TTATATATCTCAGAAGAACG+AGG	-	chr8_2:2265589-2265608	None:intergenic	30.0%
ATCACAACTTCATATAACGT+AGG	-	chr8_2:2265701-2265720	None:intergenic	30.0%
TACGTTATATGAAGTTGTGA+TGG	+	chr8_2:2265700-2265719	Msa1227180:CDS	30.0%
ATGGAGATGATTGACAAAAT+GGG	+	chr8_2:2265719-2265738	Msa1227180:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	2263802	2267331	2263802	ID=Msa1227180;Name=Msa1227180
chr8_2	mRNA	2263802	2267331	2263802	ID=Msa1227180-mRNA-1;Parent=Msa1227180;Name=Msa1227180-mRNA-1;_AED=0.13;_eAED=0.13;_QI=181\|0.9\|0.90\|1\|0.9\|0.81\|11\|246\|346
chr8_2	exon	2263802	2263969	2263802	ID=Msa1227180-mRNA-1:exon:535;Parent=Msa1227180-mRNA-1
chr8_2	exon	2264736	2265034	2264736	ID=Msa1227180-mRNA-1:exon:536;Parent=Msa1227180-mRNA-1
chr8_2	exon	2265184	2265354	2265184	ID=Msa1227180-mRNA-1:exon:537;Parent=Msa1227180-mRNA-1
chr8_2	exon	2265473	2265522	2265473	ID=Msa1227180-mRNA-1:exon:538;Parent=Msa1227180-mRNA-1
chr8_2	exon	2265692	2265748	2265692	ID=Msa1227180-mRNA-1:exon:539;Parent=Msa1227180-mRNA-1
chr8_2	exon	2265838	2265903	2265838	ID=Msa1227180-mRNA-1:exon:540;Parent=Msa1227180-mRNA-1
chr8_2	exon	2266010	2266084	2266010	ID=Msa1227180-mRNA-1:exon:541;Parent=Msa1227180-mRNA-1
chr8_2	exon	2266284	2266402	2266284	ID=Msa1227180-mRNA-1:exon:542;Parent=Msa1227180-mRNA-1
chr8_2	exon	2266511	2266602	2266511	ID=Msa1227180-mRNA-1:exon:543;Parent=Msa1227180-mRNA-1
chr8_2	exon	2266890	2267007	2266890	ID=Msa1227180-mRNA-1:exon:544;Parent=Msa1227180-mRNA-1
chr8_2	exon	2267079	2267331	2267079	ID=Msa1227180-mRNA-1:exon:545;Parent=Msa1227180-mRNA-1
chr8_2	five_prime_UTR	2263802	2263969	2263802	ID=Msa1227180-mRNA-1:five_prime_utr;Parent=Msa1227180-mRNA-1
chr8_2	five_prime_UTR	2264736	2264748	2264736	ID=Msa1227180-mRNA-1:five_prime_utr;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2264749	2265034	2264749	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2265184	2265354	2265184	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2265473	2265522	2265473	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2265692	2265748	2265692	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2265838	2265903	2265838	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2266010	2266084	2266010	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2266284	2266402	2266284	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2266511	2266602	2266511	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2266890	2267007	2266890	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	CDS	2267079	2267085	2267079	ID=Msa1227180-mRNA-1:cds;Parent=Msa1227180-mRNA-1
chr8_2	three_prime_UTR	2267086	2267331	2267086	ID=Msa1227180-mRNA-1:three_prime_utr;Parent=Msa1227180-mRNA-1

Gene Sequence

>Msa1227180

ATGGCTTCTCTTAAAGGACCAGTTTTCTGTCCCTCTATTCATCCTAAAGTTGCAGGGTTCTGCACCCTTCCTATGATTGGACCAATGAATGTTAGATGTGTTAGAACTGAGTTTTGGGGGCTAAGGGAATTTAAGAGGAGTTTCCTTTCTTACCACATAAACACGCGGAAGAGCAATACAGTAGTGCATTGTAGTTTAAATTCGCCCTCGAATGATGGTGGAAGTATGGCAGAAAGTTTCAATGAAAAAGATGAAGATTATGTTAGTTCAAGTGTGATTGAAGCCGTTGAGGTGAAGAGTGGAGCAGATGGTTTCATAATCAAAATGAGGGATGGAAAACACTTGAGATGTGTCCATAACAATCCTCAGGGAGGTCATCTTCCTGATTATTCACCACATCCCGCTATGGTTTTGAAAATGGAAGATGGGTCTGGCTTACTTCTACCTATAATTGTTTTGGAGATGCCAAGCGTCTTACTACTGGCAGCAGTATGCAATGTTTCAATTGCTAGGCCTACGTTATATGAAGTTGTGATGGAGATGATTGACAAAATGGGTTACCAAGTCAGACTTGTCAGAGTTACTAGGAGAGTCGATGAGGCATATTTTGCTCAGTTGTATCTTACCAAGGTTGGAAATGAGGCAGAATGTAGGAGCTTTGACCTTCGACCTTCTGATGCTATCAATATTGCAGTAAGATGCAAGGTGCCAATACAAGTCAACAAGTACTTGGTGTCCAGTGATGGAATGAGAGTAATTGAATCAGGCAAATTGCCAACGCAGATCCCTAGTTTTGACGGTCGATTATTTACTGAAATGGACAAGCCAAATGGTCAACCTTGTGCTGAAACTGATGAATTTAATCTTTTGAACAACATGTTGAAAGCTGTTGATGAAGAGCGTTACGATGATGCCGAATATAAATTGTACTTCTCTGATCTTGCTACTTTCACCTCTCATGTTTCTGATGCAACTCCAGCATTGTGGAGGGACAGGCTTAATCAACATAGGGCTGGTAAAAACATGAAAAGAAGGTCCTGA

Protein sequence

>Msa1227180

MASLKGPVFCPSIHPKVAGFCTLPMIGPMNVRCVRTEFWGLREFKRSFLSYHINTRKSNTVVHCSLNSPSNDGGSMAESFNEKDEDYVSSSVIEAVEVKSGADGFIIKMRDGKHLRCVHNNPQGGHLPDYSPHPAMVLKMEDGSGLLLPIIVLEMPSVLLLAAVCNVSIARPTLYEVVMEMIDKMGYQVRLVRVTRRVDEAYFAQLYLTKVGNEAECRSFDLRPSDAINIAVRCKVPIQVNKYLVSSDGMRVIESGKLPTQIPSFDGRLFTEMDKPNGQPCAETDEFNLLNNMLKAVDEERYDDAEYKLYFSDLATFTSHVSDATPALWRDRLNQHRAGKNMKRRS*