Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1232510 | XP_039685435.1 | 96.789 | 218 | 7 | 0 | 1 | 218 | 1 | 218 | 1.79e-154 | 437 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1232510 | sp|O82155|DOF17_ARATH | 56.643 | 143 | 55 | 2 | 5 | 142 | 18 | 158 | 4.02e-45 | 151 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa1232510 | TF | C2C2-Dof |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1133310 | Msa1232510 | 0.885795 | 5.976065e-72 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1232510 | MtrunA17_Chr8g0341671 | 96.789 | 218 | 7 | 0 | 1 | 218 | 1 | 218 | 1.64e-158 | 437 |
| Msa1232510 | MtrunA17_Chr8g0374111 | 56.667 | 120 | 50 | 2 | 15 | 132 | 24 | 143 | 1.92e-36 | 127 |
| Msa1232510 | MtrunA17_Chr7g0251181 | 59.124 | 137 | 24 | 3 | 1 | 137 | 1 | 105 | 2.36e-36 | 124 |
| Msa1232510 | MtrunA17_Chr2g0328021 | 51.145 | 131 | 57 | 3 | 15 | 143 | 57 | 182 | 8.54e-36 | 127 |
| Msa1232510 | MtrunA17_Chr4g0031411 | 61.628 | 86 | 32 | 1 | 15 | 100 | 70 | 154 | 6.34e-32 | 118 |
| Msa1232510 | MtrunA17_Chr8g0374251 | 81.818 | 55 | 10 | 0 | 17 | 71 | 65 | 119 | 1.90e-30 | 114 |
| Msa1232510 | MtrunA17_Chr7g0223311 | 75.385 | 65 | 16 | 0 | 21 | 85 | 78 | 142 | 2.43e-30 | 115 |
| Msa1232510 | MtrunA17_Chr2g0308011 | 50.000 | 122 | 47 | 2 | 20 | 135 | 37 | 150 | 3.77e-30 | 110 |
| Msa1232510 | MtrunA17_Chr4g0047001 | 65.000 | 80 | 26 | 1 | 21 | 98 | 56 | 135 | 4.39e-30 | 114 |
| Msa1232510 | MtrunA17_Chr4g0030051 | 75.000 | 64 | 16 | 0 | 8 | 71 | 29 | 92 | 5.15e-30 | 112 |
| Msa1232510 | MtrunA17_Chr7g0237921 | 60.638 | 94 | 35 | 2 | 13 | 104 | 68 | 161 | 1.65e-29 | 112 |
| Msa1232510 | MtrunA17_Chr4g0061761 | 84.906 | 53 | 8 | 0 | 18 | 70 | 38 | 90 | 2.78e-29 | 111 |
| Msa1232510 | MtrunA17_Chr4g0010151 | 52.679 | 112 | 38 | 3 | 13 | 111 | 74 | 183 | 2.79e-29 | 112 |
| Msa1232510 | MtrunA17_Chr8g0346551 | 54.545 | 88 | 40 | 0 | 17 | 104 | 21 | 108 | 5.72e-29 | 109 |
| Msa1232510 | MtrunA17_Chr5g0411951 | 75.000 | 56 | 14 | 0 | 15 | 70 | 38 | 93 | 1.38e-28 | 109 |
| Msa1232510 | MtrunA17_Chr8g0367161 | 62.821 | 78 | 25 | 1 | 20 | 97 | 32 | 105 | 1.55e-28 | 109 |
| Msa1232510 | MtrunA17_Chr3g0127201 | 71.875 | 64 | 16 | 2 | 15 | 77 | 21 | 83 | 1.10e-27 | 106 |
| Msa1232510 | MtrunA17_Chr1g0188851 | 68.750 | 64 | 20 | 0 | 11 | 74 | 34 | 97 | 1.43e-27 | 106 |
| Msa1232510 | MtrunA17_Chr2g0282511 | 87.755 | 49 | 6 | 0 | 20 | 68 | 45 | 93 | 1.69e-27 | 106 |
| Msa1232510 | MtrunA17_Chr3g0117601 | 76.786 | 56 | 13 | 0 | 15 | 70 | 56 | 111 | 6.14e-27 | 105 |
| Msa1232510 | MtrunA17_Chr2g0326001 | 68.750 | 64 | 20 | 0 | 8 | 71 | 28 | 91 | 8.78e-27 | 103 |
| Msa1232510 | MtrunA17_Chr3g0126201 | 84.000 | 50 | 8 | 0 | 22 | 71 | 53 | 102 | 1.69e-26 | 103 |
| Msa1232510 | MtrunA17_Chr6g0453321 | 60.870 | 69 | 27 | 0 | 20 | 88 | 136 | 204 | 3.79e-26 | 105 |
| Msa1232510 | MtrunA17_Chr4g0047761 | 77.358 | 53 | 12 | 0 | 18 | 70 | 70 | 122 | 6.85e-26 | 102 |
| Msa1232510 | MtrunA17_Chr2g0282441 | 78.431 | 51 | 11 | 0 | 21 | 71 | 26 | 76 | 1.57e-25 | 101 |
| Msa1232510 | MtrunA17_Chr2g0283811 | 67.857 | 56 | 18 | 0 | 17 | 72 | 44 | 99 | 1.93e-25 | 97.4 |
| Msa1232510 | MtrunA17_Chr1g0177251 | 82.000 | 50 | 9 | 0 | 22 | 71 | 14 | 63 | 2.02e-25 | 100 |
| Msa1232510 | MtrunA17_Chr2g0282051 | 67.742 | 62 | 17 | 1 | 9 | 67 | 32 | 93 | 3.97e-25 | 99.4 |
| Msa1232510 | MtrunA17_Chr7g0217581 | 62.857 | 70 | 25 | 1 | 20 | 89 | 232 | 300 | 4.68e-25 | 102 |
| Msa1232510 | MtrunA17_Chr7g0254161 | 35.519 | 183 | 98 | 4 | 20 | 185 | 101 | 280 | 1.04e-24 | 100 |
| Msa1232510 | MtrunA17_Chr3g0092111 | 57.143 | 77 | 33 | 0 | 20 | 96 | 136 | 212 | 1.50e-24 | 100 |
| Msa1232510 | MtrunA17_Chr5g0417601 | 59.211 | 76 | 27 | 1 | 9 | 84 | 133 | 204 | 2.39e-24 | 99.0 |
| Msa1232510 | MtrunA17_Chr8g0355261 | 69.231 | 52 | 16 | 0 | 20 | 71 | 118 | 169 | 5.16e-24 | 98.6 |
| Msa1232510 | MtrunA17_Chr5g0417571 | 62.500 | 64 | 23 | 1 | 8 | 71 | 127 | 189 | 5.27e-24 | 97.4 |
| Msa1232510 | MtrunA17_Chr6g0460731 | 47.561 | 82 | 43 | 0 | 20 | 101 | 96 | 177 | 5.55e-24 | 97.8 |
| Msa1232510 | MtrunA17_Chr4g0042481 | 69.231 | 52 | 16 | 0 | 20 | 71 | 122 | 173 | 1.99e-23 | 97.4 |
| Msa1232510 | MtrunA17_Chr6g0460721 | 45.977 | 87 | 47 | 0 | 20 | 106 | 89 | 175 | 5.15e-23 | 94.7 |
| Msa1232510 | MtrunA17_Chr5g0417551 | 53.409 | 88 | 35 | 1 | 20 | 101 | 127 | 214 | 6.24e-23 | 95.1 |
| Msa1232510 | MtrunA17_Chr5g0417561 | 56.471 | 85 | 29 | 2 | 20 | 96 | 119 | 203 | 2.92e-22 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1232510 | AT1G51700.1 | 56.643 | 143 | 55 | 2 | 5 | 142 | 18 | 158 | 4.09e-46 | 151 |
| Msa1232510 | AT3G21270.1 | 51.007 | 149 | 63 | 4 | 20 | 158 | 29 | 177 | 2.44e-33 | 119 |
| Msa1232510 | AT5G60850.1 | 64.368 | 87 | 27 | 1 | 16 | 102 | 47 | 129 | 9.10e-33 | 120 |
| Msa1232510 | AT3G50410.1 | 73.973 | 73 | 13 | 2 | 10 | 76 | 14 | 86 | 1.10e-32 | 119 |
| Msa1232510 | AT5G66940.1 | 70.423 | 71 | 21 | 0 | 16 | 86 | 28 | 98 | 2.45e-32 | 117 |
| Msa1232510 | AT5G60200.1 | 59.302 | 86 | 33 | 1 | 15 | 98 | 50 | 135 | 1.23e-30 | 114 |
| Msa1232510 | AT1G07640.1 | 73.438 | 64 | 17 | 0 | 15 | 78 | 16 | 79 | 2.69e-30 | 113 |
| Msa1232510 | AT3G45610.1 | 68.831 | 77 | 20 | 3 | 11 | 85 | 32 | 106 | 2.92e-30 | 112 |
| Msa1232510 | AT1G07640.2 | 73.438 | 64 | 17 | 0 | 15 | 78 | 72 | 135 | 5.29e-30 | 114 |
| Msa1232510 | AT5G65590.1 | 69.014 | 71 | 21 | 1 | 16 | 85 | 37 | 107 | 6.02e-30 | 113 |
| Msa1232510 | AT1G07640.3 | 73.438 | 64 | 17 | 0 | 15 | 78 | 80 | 143 | 6.73e-30 | 113 |
| Msa1232510 | AT4G38000.1 | 58.242 | 91 | 33 | 1 | 11 | 101 | 32 | 117 | 2.42e-29 | 110 |
| Msa1232510 | AT2G28810.2 | 75.439 | 57 | 14 | 0 | 15 | 71 | 72 | 128 | 4.12e-29 | 111 |
| Msa1232510 | AT2G28810.1 | 75.439 | 57 | 14 | 0 | 15 | 71 | 89 | 145 | 5.84e-29 | 111 |
| Msa1232510 | AT3G52440.1 | 55.208 | 96 | 39 | 1 | 22 | 113 | 27 | 122 | 1.31e-28 | 108 |
| Msa1232510 | AT3G52440.2 | 55.208 | 96 | 39 | 1 | 22 | 113 | 46 | 141 | 2.25e-28 | 108 |
| Msa1232510 | AT1G21340.1 | 50.980 | 102 | 47 | 1 | 16 | 114 | 31 | 132 | 2.45e-28 | 107 |
| Msa1232510 | AT2G37590.1 | 75.862 | 58 | 13 | 1 | 14 | 71 | 84 | 140 | 4.64e-28 | 108 |
| Msa1232510 | AT3G55370.1 | 39.735 | 151 | 69 | 4 | 14 | 144 | 71 | 219 | 5.25e-28 | 108 |
| Msa1232510 | AT3G55370.3 | 39.610 | 154 | 71 | 4 | 11 | 144 | 113 | 264 | 5.61e-28 | 108 |
| Msa1232510 | AT3G61850.2 | 63.889 | 72 | 19 | 1 | 17 | 88 | 59 | 123 | 5.93e-28 | 107 |
| Msa1232510 | AT3G61850.3 | 63.889 | 72 | 19 | 1 | 17 | 88 | 59 | 123 | 5.93e-28 | 107 |
| Msa1232510 | AT3G55370.2 | 39.735 | 151 | 69 | 4 | 14 | 144 | 71 | 219 | 6.80e-28 | 108 |
| Msa1232510 | AT3G61850.1 | 63.889 | 72 | 19 | 1 | 17 | 88 | 71 | 135 | 7.21e-28 | 107 |
| Msa1232510 | AT3G61850.5 | 63.889 | 72 | 19 | 1 | 17 | 88 | 59 | 123 | 9.12e-28 | 107 |
| Msa1232510 | AT3G61850.4 | 63.889 | 72 | 19 | 1 | 17 | 88 | 71 | 135 | 1.02e-27 | 107 |
| Msa1232510 | AT1G28310.1 | 84.615 | 52 | 8 | 0 | 20 | 71 | 27 | 78 | 1.45e-27 | 107 |
| Msa1232510 | AT1G28310.2 | 84.615 | 52 | 8 | 0 | 20 | 71 | 51 | 102 | 1.88e-27 | 107 |
| Msa1232510 | AT2G28510.1 | 75.806 | 62 | 14 | 1 | 18 | 79 | 46 | 106 | 3.53e-27 | 105 |
| Msa1232510 | AT4G24060.1 | 74.138 | 58 | 14 | 1 | 15 | 71 | 47 | 104 | 1.52e-26 | 104 |
| Msa1232510 | AT5G62940.1 | 76.786 | 56 | 13 | 0 | 15 | 70 | 68 | 123 | 1.72e-26 | 105 |
| Msa1232510 | AT5G02460.1 | 71.667 | 60 | 16 | 1 | 13 | 71 | 87 | 146 | 6.51e-26 | 103 |
| Msa1232510 | AT1G47655.1 | 39.899 | 198 | 101 | 5 | 5 | 192 | 11 | 200 | 7.13e-26 | 100 |
| Msa1232510 | AT4G00940.1 | 72.414 | 58 | 16 | 0 | 17 | 74 | 65 | 122 | 1.64e-25 | 101 |
| Msa1232510 | AT4G00940.3 | 72.414 | 58 | 16 | 0 | 17 | 74 | 65 | 122 | 1.64e-25 | 101 |
| Msa1232510 | AT4G00940.2 | 72.414 | 58 | 16 | 0 | 17 | 74 | 65 | 122 | 1.64e-25 | 101 |
| Msa1232510 | AT2G46590.1 | 76.471 | 51 | 12 | 0 | 17 | 67 | 65 | 115 | 2.25e-25 | 102 |
| Msa1232510 | AT2G46590.2 | 76.471 | 51 | 12 | 0 | 17 | 67 | 77 | 127 | 2.64e-25 | 102 |
| Msa1232510 | AT1G64620.1 | 75.000 | 56 | 13 | 1 | 15 | 69 | 43 | 98 | 3.71e-25 | 101 |
| Msa1232510 | AT4G21050.1 | 80.000 | 50 | 10 | 0 | 22 | 71 | 26 | 75 | 3.98e-25 | 98.2 |
| Msa1232510 | AT2G34140.1 | 73.077 | 52 | 14 | 0 | 20 | 71 | 58 | 109 | 4.29e-25 | 97.1 |
| Msa1232510 | AT1G29160.1 | 73.077 | 52 | 14 | 0 | 20 | 71 | 62 | 113 | 4.33e-25 | 97.1 |
| Msa1232510 | AT1G69570.1 | 73.077 | 52 | 14 | 0 | 20 | 71 | 132 | 183 | 7.39e-25 | 100 |
| Msa1232510 | AT3G47500.1 | 61.644 | 73 | 27 | 1 | 20 | 92 | 110 | 181 | 1.02e-24 | 101 |
| Msa1232510 | AT5G39660.3 | 59.420 | 69 | 28 | 0 | 20 | 88 | 138 | 206 | 1.09e-23 | 98.2 |
| Msa1232510 | AT5G39660.1 | 59.420 | 69 | 28 | 0 | 20 | 88 | 138 | 206 | 1.09e-23 | 98.2 |
| Msa1232510 | AT5G39660.2 | 59.420 | 69 | 28 | 0 | 20 | 88 | 138 | 206 | 1.09e-23 | 98.2 |
| Msa1232510 | AT1G26790.1 | 69.231 | 52 | 16 | 0 | 20 | 71 | 111 | 162 | 2.44e-23 | 96.7 |
| Msa1232510 | AT5G62430.1 | 69.231 | 52 | 16 | 0 | 20 | 71 | 54 | 105 | 6.91e-23 | 94.4 |
| Msa1232510 | AT4G21040.1 | 68.519 | 54 | 17 | 0 | 22 | 75 | 27 | 80 | 7.72e-23 | 92.8 |
| Msa1232510 | AT4G21080.1 | 70.370 | 54 | 16 | 0 | 22 | 75 | 27 | 80 | 2.32e-22 | 91.7 |
| Msa1232510 | AT4G21030.1 | 62.000 | 50 | 19 | 0 | 22 | 71 | 23 | 72 | 3.68e-14 | 68.6 |
Find 60 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGACTAAAGGTGGTGCTTTA+AGG | 0.163122 | 8_2:-8972284 | Msa1232510:CDS |
| TGTTCTTGTTCTGGAAAATT+TGG | 0.207790 | 8_2:+8972411 | None:intergenic |
| ATCTTTCTTGGTTTGCAAAA+TGG | 0.257606 | 8_2:-8971886 | Msa1232510:CDS |
| CTTGCATCAACTGAGCGATT+TGG | 0.269361 | 8_2:-8972051 | Msa1232510:CDS |
| TTCTGGAAAATTTGGTTTCA+TGG | 0.271012 | 8_2:+8972419 | None:intergenic |
| CATTTGAGTTGTTCTTGTTC+TGG | 0.291270 | 8_2:+8972402 | None:intergenic |
| CCCGGTTGTGAATACGGATT+TGG | 0.293449 | 8_2:-8971944 | Msa1232510:CDS |
| GGGGAATCAGAGATCTTTCT+TGG | 0.299700 | 8_2:-8971898 | Msa1232510:CDS |
| CACCCGGGTCAACATGAATC+TGG | 0.303329 | 8_2:+8972109 | None:intergenic |
| TTGCATCAACTGAGCGATTT+GGG | 0.303955 | 8_2:-8972050 | Msa1232510:CDS |
| AAATGGGTCGGGTTTGAAAA+TGG | 0.305819 | 8_2:-8972004 | Msa1232510:CDS |
| GACCCGGGTGAGAGGAATTT+TGG | 0.316050 | 8_2:-8972096 | Msa1232510:CDS |
| TTGAATTCAGACCTTCAAAA+AGG | 0.316361 | 8_2:+8972025 | None:intergenic |
| CATAACCCGGGTGGTAGAAA+TGG | 0.353048 | 8_2:-8971919 | Msa1232510:CDS |
| GGGTTTGAAAATGGTGGAAA+TGG | 0.363325 | 8_2:-8971995 | Msa1232510:CDS |
| TTTGAAGGTCTGAATTCAAA+TGG | 0.368494 | 8_2:-8972021 | Msa1232510:CDS |
| TCATGGGTTGAAATGTTGTT+TGG | 0.390520 | 8_2:+8972436 | None:intergenic |
| ATGGTGGAAATGGGTGAATT+TGG | 0.397617 | 8_2:-8971985 | Msa1232510:CDS |
| CCCAAATCCGTATTCACAAC+CGG | 0.400363 | 8_2:+8971943 | None:intergenic |
| ATAACCCGGGTGGTAGAAAT+GGG | 0.416940 | 8_2:-8971918 | Msa1232510:CDS |
| AGATGGTGTTGTTGAACGTT+TGG | 0.432656 | 8_2:+8972209 | None:intergenic |
| CTGTGACCCGGTTGTGAATA+CGG | 0.450073 | 8_2:-8971950 | Msa1232510:CDS |
| TTTCTTGTTCCACCACCAAC+TGG | 0.453104 | 8_2:+8972255 | None:intergenic |
| CCGGTTGTGAATACGGATTT+GGG | 0.453312 | 8_2:-8971943 | Msa1232510:CDS |
| TGCAAAAGATATTGGACTAA+AGG | 0.473852 | 8_2:-8972297 | Msa1232510:CDS |
| TCTGGAAAATTTGGTTTCAT+GGG | 0.485487 | 8_2:+8972420 | None:intergenic |
| CCACCAAAATTCCTCTCACC+CGG | 0.488376 | 8_2:+8972093 | None:intergenic |
| TACGGATTTGGGTCATAACC+CGG | 0.489454 | 8_2:-8971932 | Msa1232510:CDS |
| TTGAAGGTCTGAATTCAAAT+GGG | 0.494568 | 8_2:-8972020 | Msa1232510:CDS |
| GGTTTGAAAATGGTGGAAAT+GGG | 0.507050 | 8_2:-8971994 | Msa1232510:CDS |
| GGTCTGAATTCAAATGGGTC+GGG | 0.510843 | 8_2:-8972015 | Msa1232510:CDS |
| TTTCTTGGTTTGCAAAATGG+TGG | 0.514745 | 8_2:-8971883 | Msa1232510:CDS |
| AGGTCTGAATTCAAATGGGT+CGG | 0.529995 | 8_2:-8972016 | Msa1232510:CDS |
| AACTGTTGGAATGATAATCA+TGG | 0.536064 | 8_2:-8971856 | Msa1232510:CDS |
| GACCCAGATTCATGTTGACC+CGG | 0.541258 | 8_2:-8972112 | Msa1232510:CDS |
| GGTCAACATGAATCTGGGTC+GGG | 0.542667 | 8_2:+8972115 | None:intergenic |
| GATTCCCCATTTCTACCACC+CGG | 0.543789 | 8_2:+8971913 | None:intergenic |
| ACCCGGGTCAACATGAATCT+GGG | 0.549644 | 8_2:+8972110 | None:intergenic |
| TTCATGTTGACCCGGGTGAG+AGG | 0.558997 | 8_2:-8972104 | Msa1232510:CDS |
| ATCTTGCTATATACACTCCT+AGG | 0.564156 | 8_2:-8971825 | Msa1232510:CDS |
| AAAATGGTGGTTCAAACTGT+TGG | 0.566836 | 8_2:-8971870 | Msa1232510:CDS |
| AGGAACATACCAGTTGGTGG+TGG | 0.578156 | 8_2:-8972264 | Msa1232510:CDS |
| TGAGAATTTGAACTGTGACC+CGG | 0.580599 | 8_2:-8971962 | Msa1232510:CDS |
| GGGTCAACATGAATCTGGGT+CGG | 0.590125 | 8_2:+8972114 | None:intergenic |
| CCAAATCCGTATTCACAACC+GGG | 0.603508 | 8_2:+8971944 | None:intergenic |
| TTAAGGAACATACCAGTTGG+TGG | 0.605626 | 8_2:-8972267 | Msa1232510:CDS |
| GAACTGGTGGTTGATGCTGA+TGG | 0.606565 | 8_2:+8972177 | None:intergenic |
| AAAAGATATTGGACTAAAGG+TGG | 0.614735 | 8_2:-8972294 | Msa1232510:CDS |
| GCTTTAAGGAACATACCAGT+TGG | 0.625252 | 8_2:-8972270 | Msa1232510:CDS |
| ACGGATTTGGGTCATAACCC+GGG | 0.629532 | 8_2:-8971931 | Msa1232510:CDS |
| ACCCAGATTCATGTTGACCC+GGG | 0.637927 | 8_2:-8972111 | Msa1232510:CDS |
| ATTCCCCATTTCTACCACCC+GGG | 0.643173 | 8_2:+8971914 | None:intergenic |
| ATCTACTGATAAGTTGACCT+AGG | 0.648432 | 8_2:+8971808 | None:intergenic |
| CACCAAAATTCCTCTCACCC+GGG | 0.649447 | 8_2:+8972094 | None:intergenic |
| GTTGGAATGATAATCATGGT+TGG | 0.652232 | 8_2:-8971852 | Msa1232510:CDS |
| GCTGATGGAGAAGATGAAGA+TGG | 0.658893 | 8_2:+8972192 | None:intergenic |
| TGGGTCGGGTTTGAAAATGG+TGG | 0.664103 | 8_2:-8972001 | Msa1232510:CDS |
| TAACCCGGGTGGTAGAAATG+GGG | 0.710853 | 8_2:-8971917 | Msa1232510:CDS |
| GTGTTGCTTGATTCACAACG+TGG | 0.731087 | 8_2:+8972378 | None:intergenic |
| GATTTGGGTCATAACCCGGG+TGG | 0.768929 | 8_2:-8971928 | Msa1232510:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTTGCAGTTTTTGCAAAAA+TGG | + | chr8_2:8971944-8971963 | None:intergenic | 25.0% |
| ! | TCTGGAAAATTTGGTTTCAT+GGG | + | chr8_2:8971837-8971856 | None:intergenic | 30.0% |
| ! | TTCTGGAAAATTTGGTTTCA+TGG | + | chr8_2:8971838-8971857 | None:intergenic | 30.0% |
| !! | TGTTCTTGTTCTGGAAAATT+TGG | + | chr8_2:8971846-8971865 | None:intergenic | 30.0% |
| GCAAAAACTGCAAAAGATAT+TGG | - | chr8_2:8971949-8971968 | Msa1232510:CDS | 30.0% | |
| TGCAAAAGATATTGGACTAA+AGG | - | chr8_2:8971957-8971976 | Msa1232510:CDS | 30.0% | |
| AAAAGATATTGGACTAAAGG+TGG | - | chr8_2:8971960-8971979 | Msa1232510:CDS | 30.0% | |
| TTGAATTCAGACCTTCAAAA+AGG | + | chr8_2:8972232-8972251 | None:intergenic | 30.0% | |
| TTTGAAGGTCTGAATTCAAA+TGG | - | chr8_2:8972233-8972252 | Msa1232510:CDS | 30.0% | |
| TTGAAGGTCTGAATTCAAAT+GGG | - | chr8_2:8972234-8972253 | Msa1232510:CDS | 30.0% | |
| !! | ATCTTTCTTGGTTTGCAAAA+TGG | - | chr8_2:8972368-8972387 | Msa1232510:CDS | 30.0% |
| AACTGTTGGAATGATAATCA+TGG | - | chr8_2:8972398-8972417 | Msa1232510:CDS | 30.0% | |
| ! | TCATGGGTTGAAATGTTGTT+TGG | + | chr8_2:8971821-8971840 | None:intergenic | 35.0% |
| !! | CATTTGAGTTGTTCTTGTTC+TGG | + | chr8_2:8971855-8971874 | None:intergenic | 35.0% |
| ! | GGTTTGAAAATGGTGGAAAT+GGG | - | chr8_2:8972260-8972279 | Msa1232510:CDS | 35.0% |
| !! | TTTCTTGGTTTGCAAAATGG+TGG | - | chr8_2:8972371-8972390 | Msa1232510:CDS | 35.0% |
| AAAATGGTGGTTCAAACTGT+TGG | - | chr8_2:8972384-8972403 | Msa1232510:CDS | 35.0% | |
| GTTGGAATGATAATCATGGT+TGG | - | chr8_2:8972402-8972421 | Msa1232510:CDS | 35.0% | |
| ! | ATCTTGCTATATACACTCCT+AGG | - | chr8_2:8972429-8972448 | Msa1232510:CDS | 35.0% |
| ! | CAGTTTTTGCAAAAATGGCG+TGG | + | chr8_2:8971939-8971958 | None:intergenic | 40.0% |
| !! | GCTTTAAGGAACATACCAGT+TGG | - | chr8_2:8971984-8972003 | Msa1232510:CDS | 40.0% |
| TTAAGGAACATACCAGTTGG+TGG | - | chr8_2:8971987-8972006 | Msa1232510:CDS | 40.0% | |
| !! | AGATGGTGTTGTTGAACGTT+TGG | + | chr8_2:8972048-8972067 | None:intergenic | 40.0% |
| ! | AACTTTTGCTGCTGAAGAAC+TGG | + | chr8_2:8972096-8972115 | None:intergenic | 40.0% |
| TTGCATCAACTGAGCGATTT+GGG | - | chr8_2:8972204-8972223 | Msa1232510:CDS | 40.0% | |
| !! | CGATTTGGGAACCTTTTTGA+AGG | - | chr8_2:8972218-8972237 | Msa1232510:CDS | 40.0% |
| AGGTCTGAATTCAAATGGGT+CGG | - | chr8_2:8972238-8972257 | Msa1232510:CDS | 40.0% | |
| AAATGGGTCGGGTTTGAAAA+TGG | - | chr8_2:8972250-8972269 | Msa1232510:CDS | 40.0% | |
| ! | GGGTTTGAAAATGGTGGAAA+TGG | - | chr8_2:8972259-8972278 | Msa1232510:CDS | 40.0% |
| ! | ATGGTGGAAATGGGTGAATT+TGG | - | chr8_2:8972269-8972288 | Msa1232510:CDS | 40.0% |
| TGAGAATTTGAACTGTGACC+CGG | - | chr8_2:8972292-8972311 | Msa1232510:CDS | 40.0% | |
| !! | GTGTTGCTTGATTCACAACG+TGG | + | chr8_2:8971879-8971898 | None:intergenic | 45.0% |
| GGACTAAAGGTGGTGCTTTA+AGG | - | chr8_2:8971970-8971989 | Msa1232510:CDS | 45.0% | |
| TTTCTTGTTCCACCACCAAC+TGG | + | chr8_2:8972002-8972021 | None:intergenic | 45.0% | |
| GCTGATGGAGAAGATGAAGA+TGG | + | chr8_2:8972065-8972084 | None:intergenic | 45.0% | |
| ! | TTTTGCTGCTGAAGAACTGG+TGG | + | chr8_2:8972093-8972112 | None:intergenic | 45.0% |
| CTTGCATCAACTGAGCGATT+TGG | - | chr8_2:8972203-8972222 | Msa1232510:CDS | 45.0% | |
| GGTCTGAATTCAAATGGGTC+GGG | - | chr8_2:8972239-8972258 | Msa1232510:CDS | 45.0% | |
| CCAAATCCGTATTCACAACC+GGG | + | chr8_2:8972313-8972332 | None:intergenic | 45.0% | |
| CCCAAATCCGTATTCACAAC+CGG | + | chr8_2:8972314-8972333 | None:intergenic | 45.0% | |
| CCGGTTGTGAATACGGATTT+GGG | - | chr8_2:8972311-8972330 | Msa1232510:CDS | 45.0% | |
| TACGGATTTGGGTCATAACC+CGG | - | chr8_2:8972322-8972341 | Msa1232510:CDS | 45.0% | |
| ATAACCCGGGTGGTAGAAAT+GGG | - | chr8_2:8972336-8972355 | Msa1232510:CDS | 45.0% | |
| GGGGAATCAGAGATCTTTCT+TGG | - | chr8_2:8972356-8972375 | Msa1232510:CDS | 45.0% | |
| !! | AGGAACATACCAGTTGGTGG+TGG | - | chr8_2:8971990-8972009 | Msa1232510:CDS | 50.0% |
| ! | GAACTGGTGGTTGATGCTGA+TGG | + | chr8_2:8972080-8972099 | None:intergenic | 50.0% |
| GGTCAACATGAATCTGGGTC+GGG | + | chr8_2:8972142-8972161 | None:intergenic | 50.0% | |
| GGGTCAACATGAATCTGGGT+CGG | + | chr8_2:8972143-8972162 | None:intergenic | 50.0% | |
| GACCCAGATTCATGTTGACC+CGG | - | chr8_2:8972142-8972161 | Msa1232510:CDS | 50.0% | |
| ACCCAGATTCATGTTGACCC+GGG | - | chr8_2:8972143-8972162 | Msa1232510:CDS | 50.0% | |
| ACCCGGGTCAACATGAATCT+GGG | + | chr8_2:8972147-8972166 | None:intergenic | 50.0% | |
| CACCAAAATTCCTCTCACCC+GGG | + | chr8_2:8972163-8972182 | None:intergenic | 50.0% | |
| CCACCAAAATTCCTCTCACC+CGG | + | chr8_2:8972164-8972183 | None:intergenic | 50.0% | |
| !! | CGGGTGAGAGGAATTTTGGT+GGG | - | chr8_2:8972162-8972181 | Msa1232510:CDS | 50.0% |
| !! | GGGTGAGAGGAATTTTGGTG+GGG | - | chr8_2:8972163-8972182 | Msa1232510:CDS | 50.0% |
| !! | GGTGAGAGGAATTTTGGTGG+GGG | - | chr8_2:8972164-8972183 | Msa1232510:CDS | 50.0% |
| !! | GTGAGAGGAATTTTGGTGGG+GGG | - | chr8_2:8972165-8972184 | Msa1232510:CDS | 50.0% |
| TGGGTCGGGTTTGAAAATGG+TGG | - | chr8_2:8972253-8972272 | Msa1232510:CDS | 50.0% | |
| CTGTGACCCGGTTGTGAATA+CGG | - | chr8_2:8972304-8972323 | Msa1232510:CDS | 50.0% | |
| CCCGGTTGTGAATACGGATT+TGG | - | chr8_2:8972310-8972329 | Msa1232510:CDS | 50.0% | |
| ACGGATTTGGGTCATAACCC+GGG | - | chr8_2:8972323-8972342 | Msa1232510:CDS | 50.0% | |
| CATAACCCGGGTGGTAGAAA+TGG | - | chr8_2:8972335-8972354 | Msa1232510:CDS | 50.0% | |
| TAACCCGGGTGGTAGAAATG+GGG | - | chr8_2:8972337-8972356 | Msa1232510:CDS | 50.0% | |
| ATTCCCCATTTCTACCACCC+GGG | + | chr8_2:8972343-8972362 | None:intergenic | 50.0% | |
| GATTCCCCATTTCTACCACC+CGG | + | chr8_2:8972344-8972363 | None:intergenic | 50.0% | |
| CACCCGGGTCAACATGAATC+TGG | + | chr8_2:8972148-8972167 | None:intergenic | 55.0% | |
| TTCATGTTGACCCGGGTGAG+AGG | - | chr8_2:8972150-8972169 | Msa1232510:CDS | 55.0% | |
| ! | GACCCGGGTGAGAGGAATTT+TGG | - | chr8_2:8972158-8972177 | Msa1232510:CDS | 55.0% |
| ! | CCGGGTGAGAGGAATTTTGG+TGG | - | chr8_2:8972161-8972180 | Msa1232510:CDS | 55.0% |
| GATTTGGGTCATAACCCGGG+TGG | - | chr8_2:8972326-8972345 | Msa1232510:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 8971810 | 8972466 | 8971810 | ID=Msa1232510;Name=Msa1232510 |
| chr8_2 | mRNA | 8971810 | 8972466 | 8971810 | ID=Msa1232510-mRNA-1;Parent=Msa1232510;Name=Msa1232510-mRNA-1;_AED=0.22;_eAED=0.23;_QI=0|-1|0|1|-1|1|1|0|218 |
| chr8_2 | exon | 8971810 | 8972466 | 8971810 | ID=Msa1232510-mRNA-1:exon:3500;Parent=Msa1232510-mRNA-1 |
| chr8_2 | CDS | 8971810 | 8972466 | 8971810 | ID=Msa1232510-mRNA-1:cds;Parent=Msa1232510-mRNA-1 |
| Gene Sequence |
| Protein sequence |
