AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1233800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233800 MtrunA17_Chr8g0343451 92.500 320 20 3 1 318 1 318 0.0 565
Msa1233800 MtrunA17_Chr2g0329811 50.737 339 124 10 7 318 23 345 2.56e-87 265
Msa1233800 MtrunA17_Chr2g0300261 48.404 188 86 6 111 287 17 204 2.50e-45 154
Msa1233800 MtrunA17_Chr2g0292411 62.222 90 33 1 138 227 85 173 6.78e-33 120
Msa1233800 MtrunA17_Chr3g0103391 53.774 106 45 2 118 222 124 226 5.60e-32 121
Msa1233800 MtrunA17_Chr8g0352251 39.524 210 102 8 91 280 79 283 1.30e-31 119
Msa1233800 MtrunA17_Chr1g0177091 47.899 119 43 4 112 226 120 223 4.39e-26 105
Msa1233800 MtrunA17_Chr7g0267581 67.123 73 22 1 157 227 154 226 4.74e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233800 AT3G14020.2 45.695 302 135 13 4 294 17 300 9.71e-57 186
Msa1233800 AT3G14020.1 45.695 302 135 13 4 294 17 300 9.71e-57 186
Msa1233800 AT1G72830.1 36.970 330 177 9 4 316 23 338 3.11e-50 170
Msa1233800 AT1G72830.2 36.858 331 177 10 4 316 23 339 1.78e-48 166
Msa1233800 AT1G54160.1 48.889 225 97 7 17 230 24 241 6.58e-48 163
Msa1233800 AT1G17590.3 37.309 327 163 12 7 316 25 326 9.14e-46 158
Msa1233800 AT1G17590.2 37.309 327 163 12 7 316 25 326 9.14e-46 158
Msa1233800 AT1G17590.4 37.309 327 163 12 7 316 25 326 9.14e-46 158
Msa1233800 AT1G17590.1 37.309 327 163 12 7 316 25 326 9.14e-46 158
Msa1233800 AT1G72830.3 33.435 329 165 9 4 316 23 313 1.40e-36 134
Msa1233800 AT1G30500.2 52.632 114 53 1 118 231 52 164 1.61e-35 127
Msa1233800 AT1G30500.1 50.877 114 51 1 118 231 52 160 3.32e-34 124
Msa1233800 AT2G34720.1 47.059 119 53 2 115 227 44 158 6.79e-31 115
Msa1233800 AT5G12840.4 46.032 126 64 3 104 229 112 233 2.19e-30 116
Msa1233800 AT5G12840.2 46.032 126 64 3 104 229 112 233 2.19e-30 116
Msa1233800 AT5G12840.5 46.032 126 64 3 104 229 113 234 2.36e-30 116
Msa1233800 AT5G12840.3 46.032 126 64 3 104 229 113 234 2.36e-30 116
Msa1233800 AT5G12840.1 46.032 126 64 3 104 229 113 234 2.36e-30 116
Msa1233800 AT2G34720.2 49.123 114 48 2 120 227 14 123 6.78e-30 112
Msa1233800 AT2G34720.3 49.123 114 48 2 120 227 14 123 6.78e-30 112
Msa1233800 AT3G20910.1 45.802 131 43 4 106 222 105 221 3.04e-29 114
Msa1233800 AT5G06510.1 51.351 111 40 5 117 226 93 190 2.15e-24 100
Msa1233800 AT5G06510.5 51.351 111 40 5 117 226 93 190 2.15e-24 100
Msa1233800 AT5G06510.2 51.351 111 40 5 117 226 93 190 2.15e-24 100
Msa1233800 AT5G06510.6 51.351 111 40 5 117 226 93 190 2.15e-24 100
Msa1233800 AT5G06510.3 51.351 111 40 5 117 226 44 141 3.90e-24 98.2
Msa1233800 AT3G05690.1 46.667 135 50 6 109 235 90 210 6.44e-24 99.4
Msa1233800 AT5G06510.4 54.369 103 33 5 125 226 6 95 7.59e-24 96.3
Msa1233800 AT5G06510.7 54.369 103 33 5 125 226 6 95 7.59e-24 96.3
Msa1233800 AT3G05690.2 46.667 135 50 6 109 235 127 247 1.75e-23 99.0

Find 89 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTATTATTCAAGAATA+AGG 0.217766 8_2:-10798594 Msa1233800:three_prime_UTR
TTGTTATACTGGACAGAAAT+TGG 0.227693 8_2:+10800278 None:intergenic
TACTTTGCCTCCTTCTCAAC+TGG 0.301003 8_2:-10800171 Msa1233800:CDS
AACTGGTGGTCAATCTTATC+TGG 0.313777 8_2:-10801039 Msa1233800:CDS
TGCTTTGCATTCACGTATAT+TGG 0.351246 8_2:+10799226 None:intergenic
ACATTATTCCGTGATCCATA+TGG 0.396988 8_2:+10799824 None:intergenic
CAATGCTTGTTGCATTCCAT+TGG 0.402633 8_2:-10798665 Msa1233800:three_prime_UTR
CATATATACTTACAAGTGAT+TGG 0.407417 8_2:+10800141 None:intergenic
ATCTTATCTGGAAGTTGGTT+CGG 0.412123 8_2:-10801027 Msa1233800:CDS
GGTTCTTCACAAAGATTAAG+AGG 0.415954 8_2:+10799247 None:intergenic
TGTTCAACACTTAGCGAAAC+CGG 0.430982 8_2:-10800245 Msa1233800:CDS
TTAGAGTATGTCTCAGTATG+AGG 0.431391 8_2:+10799863 None:intergenic
CAGGTCATCTGCAGGAAGTC+AGG 0.433122 8_2:-10800198 Msa1233800:CDS
TTTGTAGCCATGAAAATGTT+TGG 0.434600 8_2:-10798543 Msa1233800:three_prime_UTR
TTCCATTGGGAAGTCATCCT+TGG 0.442295 8_2:-10798651 Msa1233800:three_prime_UTR
TGAAGTCGGATTCATGTTGT+TGG 0.453276 8_2:+10798806 None:intergenic
ATTGAGTAGAAGAAGAATCT+TGG 0.456059 8_2:+10801063 None:intergenic
AAACATTAAAGCCATGGTCT+TGG 0.460398 8_2:+10798962 None:intergenic
AAGCAGAGGAATTGTTATAC+TGG 0.467814 8_2:+10800267 None:intergenic
CATACTGAGACATACTCTAA+TGG 0.468153 8_2:-10799860 Msa1233800:CDS
TGGTTACCCTTCTCATATGC+AGG 0.470689 8_2:-10798776 Msa1233800:CDS
GGCCTACTAGCAGCTCCATA+TGG 0.471924 8_2:-10799839 Msa1233800:CDS
TCTTCTTCTACTCAATCAAC+TGG 0.473460 8_2:-10801056 Msa1233800:CDS
GTGGAAATGTGCTGGAATCA+AGG 0.474923 8_2:-10798915 Msa1233800:CDS
CTAATCATGTCTCTCACATA+AGG 0.480287 8_2:+10798698 None:intergenic
TTGAAGAGAGCTAGAGGCTC+TGG 0.481702 8_2:-10799012 Msa1233800:CDS
GCCGATGCCGGTGGTGGTGG+TGG 0.483222 8_2:-10798745 Msa1233800:CDS
TGGCGCGACTCATGTAGATA+TGG 0.485664 8_2:+10799045 None:intergenic
TCAGCCGATGCCGGTGGTGG+TGG 0.489227 8_2:-10798748 Msa1233800:CDS
CGAACGGGAGGCATTCCTAC+TGG 0.492795 8_2:+10799280 None:intergenic
CAATGAAGCACTCATGGCCA+TGG 0.498100 8_2:-10801205 Msa1233800:CDS
GGTCAATCTTATCTGGAAGT+TGG 0.499258 8_2:-10801032 Msa1233800:CDS
CAGTAGGAATGCCTCCCGTT+CGG 0.506402 8_2:-10799279 Msa1233800:CDS
TTGTCAGCCGATGCCGGTGG+TGG 0.509405 8_2:-10798751 Msa1233800:CDS
AATGCTTGTTGCATTCCATT+GGG 0.516081 8_2:-10798664 Msa1233800:three_prime_UTR
ATTCCGACCACCACCACCAC+CGG 0.525135 8_2:+10798738 None:intergenic
CAGGATTTGTCAGCCGATGC+CGG 0.529122 8_2:-10798757 Msa1233800:CDS
TTAATGTTTCCACCACTACT+CGG 0.534943 8_2:-10798948 Msa1233800:CDS
TCTCTCACATAAGGACAGAT+AGG 0.538450 8_2:+10798707 None:intergenic
ACCTTCCACACTCTTTCCGC+CGG 0.539251 8_2:+10800226 None:intergenic
TCACAAAGATTAAGAGGCAA+AGG 0.539813 8_2:+10799253 None:intergenic
AGAGGCAAAGGAATCCGAAC+GGG 0.545487 8_2:+10799265 None:intergenic
ATGGCTTGTTGAAAATCAAA+TGG 0.546725 8_2:-10798453 Msa1233800:three_prime_UTR
TGGAAATGTGCTGGAATCAA+GGG 0.553488 8_2:-10798914 Msa1233800:CDS
GAAGTTGGTTCGGAACAATC+CGG 0.553531 8_2:-10801017 Msa1233800:intron
AGAGTGTGGAAGGTATCATC+AGG 0.556743 8_2:-10800217 Msa1233800:CDS
GACAAATCCTGCATATGAGA+AGG 0.557039 8_2:+10798769 None:intergenic
AATGAAGCACTCATGGCCAT+GGG 0.561056 8_2:-10801204 Msa1233800:CDS
GGAAAACAATGAAGCACTCA+TGG 0.561079 8_2:-10801211 None:intergenic
ATCCATATGGAGCTGCTAGT+AGG 0.561398 8_2:+10799837 None:intergenic
GAATCCGAGTGGAAATGTGC+TGG 0.564823 8_2:-10798923 Msa1233800:CDS
CTCTCTTCAATGCATGCAGA+TGG 0.566697 8_2:+10799025 None:intergenic
CGGATTCAGCCGAGTAGTGG+TGG 0.574639 8_2:+10798939 None:intergenic
GGTATCATCAGGTCATCTGC+AGG 0.575460 8_2:-10800206 Msa1233800:CDS
ACTCGGATTCAGCCGAGTAG+TGG 0.577382 8_2:+10798936 None:intergenic
ACTTGTGACGTCGGAACAAG+TGG 0.585137 8_2:+10798852 None:intergenic
TCAAATGGTGTTGAATGTGT+AGG 0.593408 8_2:-10798438 Msa1233800:three_prime_UTR
CAAGTAGAAAAGTATAGAGA+TGG 0.594749 8_2:-10798886 Msa1233800:CDS
TCTTCTACTCAATCAACTGG+TGG 0.595236 8_2:-10801053 Msa1233800:CDS
CGAAACCGGCGGAAAGAGTG+TGG 0.602019 8_2:-10800231 Msa1233800:CDS
ACCGGCGGAAAGAGTGTGGA+AGG 0.603635 8_2:-10800227 Msa1233800:CDS
TAGAGTATGTCTCAGTATGA+GGG 0.603796 8_2:+10799864 None:intergenic
TTGTGATCCAGTTGAGAAGG+AGG 0.605135 8_2:+10800164 None:intergenic
GTTAATCATGCTGAGCCAGT+AGG 0.605706 8_2:-10799295 Msa1233800:CDS
TGGTGGAAACATTAAAGCCA+TGG 0.608658 8_2:+10798956 None:intergenic
AAGAGAGCTAGAGGCTCTGG+TGG 0.614598 8_2:-10799009 Msa1233800:CDS
CATGCATTGAAGAGAGCTAG+AGG 0.616928 8_2:-10799018 Msa1233800:CDS
ACATAATTCAAAATGAGCCA+AGG 0.618165 8_2:+10798634 None:intergenic
GATTCCAGCACATTTCCACT+CGG 0.619314 8_2:+10798919 None:intergenic
GCTAAGTGTTGAACAAGCAG+AGG 0.624669 8_2:+10800253 None:intergenic
TCTTGAAATTGAAAACCTAG+TGG 0.627398 8_2:+10801086 None:intergenic
ACAAATCCTGCATATGAGAA+GGG 0.629662 8_2:+10798770 None:intergenic
GATTTGTCAGCCGATGCCGG+TGG 0.632484 8_2:-10798754 Msa1233800:CDS
TGGTTGTGATCCAGTTGAGA+AGG 0.633407 8_2:+10800161 None:intergenic
TAGCAGCTCCATATGGATCA+CGG 0.633412 8_2:-10799832 Msa1233800:CDS
GGCAAAGGAATCCGAACGGG+AGG 0.635841 8_2:+10799268 None:intergenic
AGCCAAGGATGACTTCCCAA+TGG 0.637029 8_2:+10798649 None:intergenic
ATTAGAAGCACTTGTGACGT+CGG 0.640775 8_2:+10798843 None:intergenic
GTCTACGTCTCATAATAGCA+TGG 0.645173 8_2:+10799200 None:intergenic
GAATCCGACTTCAGATCATG+TGG 0.653505 8_2:-10798796 Msa1233800:CDS
AAGAGGCAAAGGAATCCGAA+CGG 0.660223 8_2:+10799264 None:intergenic
GTAACCACATGATCTGAAGT+CGG 0.661286 8_2:+10798792 None:intergenic
GACGTCGGAACAAGTGGTTG+TGG 0.668249 8_2:+10798858 None:intergenic
TGAAGCACTCATGGCCATGG+GGG 0.672419 8_2:-10801202 Msa1233800:CDS
TGCAACAAGTCTAAACCACT+AGG 0.672944 8_2:-10801101 Msa1233800:CDS
ATGAAGCACTCATGGCCATG+GGG 0.681773 8_2:-10801203 Msa1233800:CDS
ACTACTCGGCTGAATCCGAG+TGG 0.691698 8_2:-10798934 Msa1233800:CDS
CTCACATAAGGACAGATAGG+CGG 0.718305 8_2:+10798710 None:intergenic
TCAACACTTAGCGAAACCGG+CGG 0.741425 8_2:-10800242 Msa1233800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTAAGTTTTTTCCTATA+CGG + chr8_2:10799240-10799259 None:intergenic 15.0%
!! ATTTAATTACGAATAGTTAC+CGG + chr8_2:10798638-10798657 None:intergenic 20.0%
!!! AAATTCTATCTAAAATCTGT+TGG - chr8_2:10798926-10798945 Msa1233800:CDS 20.0%
!!! AATTCTATCTAAAATCTGTT+GGG - chr8_2:10798927-10798946 Msa1233800:CDS 20.0%
!!! TACAAAGTGTTTATAAATAG+AGG + chr8_2:10799611-10799630 None:intergenic 20.0%
!! AATATTTGCAAGAAAAACTA+TGG - chr8_2:10799875-10799894 Msa1233800:CDS 20.0%
!! ATCATATTTATGTCTTTATC+TGG - chr8_2:10799899-10799918 Msa1233800:intron 20.0%
!! TGTATGTATATTTCAGAATT+CGG + chr8_2:10800298-10800317 None:intergenic 20.0%
!! CTTCTTATTATTCAAGAATA+AGG - chr8_2:10801039-10801058 Msa1233800:CDS 20.0%
! AAAGTAGATTATTAGAGATG+TGG - chr8_2:10798720-10798739 Msa1233800:CDS 25.0%
! GAATTACATCAAGTCTAAAT+AGG + chr8_2:10798783-10798802 None:intergenic 25.0%
! CTGAAAATGAGTTTATAAGT+GGG - chr8_2:10799030-10799049 Msa1233800:CDS 25.0%
! AATGAAGTGATACAAATTTG+TGG - chr8_2:10799166-10799185 Msa1233800:CDS 25.0%
! AATACTCATCAATATGCATT+AGG + chr8_2:10799325-10799344 None:intergenic 25.0%
! CATATATACTTACAAGTGAT+TGG + chr8_2:10799495-10799514 None:intergenic 25.0%
!!! TTAATTTGTGATACGCTATT+TGG + chr8_2:10799634-10799653 None:intergenic 25.0%
!!! TTGCAAATATTTTTGACACA+AGG + chr8_2:10799866-10799885 None:intergenic 25.0%
!! TTGTATGTTTCTGATATTTG+AGG - chr8_2:10799930-10799949 Msa1233800:intron 25.0%
! AGAATTCGTTCAAATTCAAA+AGG + chr8_2:10800111-10800130 None:intergenic 25.0%
!! GAAGTGTTTATAGATTCTAT+TGG - chr8_2:10800205-10800224 Msa1233800:CDS 25.0%
! TGTTTATAGATTCTATTGGT+TGG - chr8_2:10800209-10800228 Msa1233800:CDS 25.0%
!!! ATCAAGCTATATTTTTGTCT+AGG - chr8_2:10801149-10801168 Msa1233800:CDS 25.0%
! AGTTAGATTTTCTGACATAG+AGG + chr8_2:10798485-10798504 None:intergenic 30.0%
TCTTGAAATTGAAAACCTAG+TGG + chr8_2:10798550-10798569 None:intergenic 30.0%
ATTGAGTAGAAGAAGAATCT+TGG + chr8_2:10798573-10798592 None:intergenic 30.0%
TTAAGAGTCACACAATTGAT+GGG - chr8_2:10799000-10799019 Msa1233800:CDS 30.0%
! CCACTTATAAACTCATTTTC+AGG + chr8_2:10799032-10799051 None:intergenic 30.0%
CCTGAAAATGAGTTTATAAG+TGG - chr8_2:10799029-10799048 Msa1233800:CDS 30.0%
TAATTCAACCCCTACAAAAT+AGG + chr8_2:10799077-10799096 None:intergenic 30.0%
! TATTTTGTAGGGGTTGAATT+AGG - chr8_2:10799076-10799095 Msa1233800:intron 30.0%
TGAGTACTCTCTTTGAATTT+GGG + chr8_2:10799107-10799126 None:intergenic 30.0%
AACTAGTATTAGAAAGCTGT+TGG - chr8_2:10799272-10799291 Msa1233800:CDS 30.0%
TATTGATGAGTATTTGCTCT+AGG - chr8_2:10799331-10799350 Msa1233800:intron 30.0%
TTGTTATACTGGACAGAAAT+TGG + chr8_2:10799358-10799377 None:intergenic 30.0%
GTATATATGTGTTTGTGTGT+TGG - chr8_2:10799506-10799525 Msa1233800:intron 30.0%
! GTAGCGGAATTTGAATAAAT+TGG + chr8_2:10799659-10799678 None:intergenic 30.0%
! TTTCTTATAGTTTGCCTTTC+AGG - chr8_2:10799733-10799752 Msa1233800:intron 30.0%
AACTTAACACATACATAAGG+CGG + chr8_2:10799975-10799994 None:intergenic 30.0%
CCAAACTTAACACATACATA+AGG + chr8_2:10799978-10799997 None:intergenic 30.0%
CCTTATGTATGTGTTAAGTT+TGG - chr8_2:10799975-10799994 Msa1233800:intron 30.0%
AAAAAGAGAAACACCTTTGT+AGG - chr8_2:10800057-10800076 Msa1233800:intron 30.0%
! AATAGCATGTTTTCCTACAA+AGG + chr8_2:10800073-10800092 None:intergenic 30.0%
! GCTAAGTAAAAGGCATAAAT+AGG + chr8_2:10800179-10800198 None:intergenic 30.0%
! CTTCTAAGAAGCTAAGTAAA+AGG + chr8_2:10800189-10800208 None:intergenic 30.0%
AGAACAAACTCGTCAAAAAT+CGG - chr8_2:10800480-10800499 Msa1233800:intron 30.0%
! TTTCTGTTTCCTTTTCTCTA+AGG - chr8_2:10800516-10800535 Msa1233800:intron 30.0%
! TTCTGTTTCCTTTTCTCTAA+GGG - chr8_2:10800517-10800536 Msa1233800:intron 30.0%
ATAAATCTCCCTTAGAGAAA+AGG + chr8_2:10800528-10800547 None:intergenic 30.0%
! CAAGTAGAAAAGTATAGAGA+TGG - chr8_2:10800747-10800766 Msa1233800:intron 30.0%
ACATAATTCAAAATGAGCCA+AGG + chr8_2:10801002-10801021 None:intergenic 30.0%
TTTGTAGCCATGAAAATGTT+TGG - chr8_2:10801090-10801109 Msa1233800:CDS 30.0%
! TCTTTAGCCAAACATTTTCA+TGG + chr8_2:10801100-10801119 None:intergenic 30.0%
! TTGATTTTCAACAAGCCATT+TGG + chr8_2:10801179-10801198 None:intergenic 30.0%
!! ATGGCTTGTTGAAAATCAAA+TGG - chr8_2:10801180-10801199 Msa1233800:CDS 30.0%
TCTTCTTCTACTCAATCAAC+TGG - chr8_2:10798577-10798596 Msa1233800:three_prime_UTR 35.0%
! ATCTTATCTGGAAGTTGGTT+CGG - chr8_2:10798606-10798625 Msa1233800:three_prime_UTR 35.0%
ACAAGAGTCAAATGCAGATA+TGG + chr8_2:10798666-10798685 None:intergenic 35.0%
AGAAAACATGATATCCACGA+AGG + chr8_2:10798900-10798919 None:intergenic 35.0%
GTTAAGAGTCACACAATTGA+TGG - chr8_2:10798999-10799018 Msa1233800:CDS 35.0%
! ACCTTACAAGCCTATTTTGT+AGG - chr8_2:10799064-10799083 Msa1233800:intron 35.0%
! CCTTACAAGCCTATTTTGTA+GGG - chr8_2:10799065-10799084 Msa1233800:intron 35.0%
! CTTACAAGCCTATTTTGTAG+GGG - chr8_2:10799066-10799085 Msa1233800:intron 35.0%
GTGAGTACTCTCTTTGAATT+TGG + chr8_2:10799108-10799127 None:intergenic 35.0%
AAGCAGAGGAATTGTTATAC+TGG + chr8_2:10799369-10799388 None:intergenic 35.0%
! ACAGTAAGTGTTGTCAATTG+TGG - chr8_2:10799539-10799558 Msa1233800:intron 35.0%
TAGAGTATGTCTCAGTATGA+GGG + chr8_2:10799772-10799791 None:intergenic 35.0%
TTAGAGTATGTCTCAGTATG+AGG + chr8_2:10799773-10799792 None:intergenic 35.0%
CATACTGAGACATACTCTAA+TGG - chr8_2:10799773-10799792 Msa1233800:intron 35.0%
ACATTATTCCGTGATCCATA+TGG + chr8_2:10799812-10799831 None:intergenic 35.0%
TCAAGTAACTCAATGGCATT+TGG + chr8_2:10800249-10800268 None:intergenic 35.0%
TGGTACATCAAGTAACTCAA+TGG + chr8_2:10800256-10800275 None:intergenic 35.0%
TTGAGTTACTTGATGTACCA+TGG - chr8_2:10800256-10800275 Msa1233800:CDS 35.0%
CATGCTATAAAAATCTTGCC+AGG - chr8_2:10800316-10800335 Msa1233800:intron 35.0%
TCACAAAGATTAAGAGGCAA+AGG + chr8_2:10800383-10800402 None:intergenic 35.0%
GGTTCTTCACAAAGATTAAG+AGG + chr8_2:10800389-10800408 None:intergenic 35.0%
TGCTTTGCATTCACGTATAT+TGG + chr8_2:10800410-10800429 None:intergenic 35.0%
AAACATTAAAGCCATGGTCT+TGG + chr8_2:10800674-10800693 None:intergenic 35.0%
TTAATGTTTCCACCACTACT+CGG - chr8_2:10800685-10800704 Msa1233800:intron 35.0%
ACAAATCCTGCATATGAGAA+GGG + chr8_2:10800866-10800885 None:intergenic 35.0%
CTAATCATGTCTCTCACATA+AGG + chr8_2:10800938-10800957 None:intergenic 35.0%
! AATGCTTGTTGCATTCCATT+GGG - chr8_2:10800969-10800988 Msa1233800:intron 35.0%
! TCAAATGGTGTTGAATGTGT+AGG - chr8_2:10801195-10801214 Msa1233800:CDS 35.0%
TGCAACAAGTCTAAACCACT+AGG - chr8_2:10798532-10798551 Msa1233800:three_prime_UTR 40.0%
TCTTCTACTCAATCAACTGG+TGG - chr8_2:10798580-10798599 Msa1233800:three_prime_UTR 40.0%
AACTGGTGGTCAATCTTATC+TGG - chr8_2:10798594-10798613 Msa1233800:three_prime_UTR 40.0%
GGTCAATCTTATCTGGAAGT+TGG - chr8_2:10798601-10798620 Msa1233800:three_prime_UTR 40.0%
! TTTTCTCTCTTCTACCTTCG+TGG - chr8_2:10798883-10798902 Msa1233800:CDS 40.0%
!! ATGTCTAGTCTTGGATGCAA+GGG - chr8_2:10798970-10798989 Msa1233800:CDS 40.0%
CCCTACAAAATAGGCTTGTA+AGG + chr8_2:10799068-10799087 None:intergenic 40.0%
CTCTGCATTGAATACCGTAT+AGG - chr8_2:10799223-10799242 Msa1233800:CDS 40.0%
TGTTCAACACTTAGCGAAAC+CGG - chr8_2:10799388-10799407 Msa1233800:intron 40.0%
AGCGACACTATAACATTGGA+AGG + chr8_2:10799584-10799603 None:intergenic 40.0%
CGATAGCGACACTATAACAT+TGG + chr8_2:10799588-10799607 None:intergenic 40.0%
GTGATAGACAGAATTCACCA+TGG + chr8_2:10800276-10800295 None:intergenic 40.0%
GTCTACGTCTCATAATAGCA+TGG + chr8_2:10800436-10800455 None:intergenic 40.0%
TGGTGGAAACATTAAAGCCA+TGG + chr8_2:10800680-10800699 None:intergenic 40.0%
TGGAAATGTGCTGGAATCAA+GGG - chr8_2:10800719-10800738 Msa1233800:intron 40.0%
!! ATTAGAAGCACTTGTGACGT+CGG + chr8_2:10800793-10800812 None:intergenic 40.0%
TGAAGTCGGATTCATGTTGT+TGG + chr8_2:10800830-10800849 None:intergenic 40.0%
! GTAACCACATGATCTGAAGT+CGG + chr8_2:10800844-10800863 None:intergenic 40.0%
GACAAATCCTGCATATGAGA+AGG + chr8_2:10800867-10800886 None:intergenic 40.0%
TCTCTCACATAAGGACAGAT+AGG + chr8_2:10800929-10800948 None:intergenic 40.0%
! CAATGCTTGTTGCATTCCAT+TGG - chr8_2:10800968-10800987 Msa1233800:intron 40.0%
!! TTTTGTCTAGGACTGCCAAA+TGG - chr8_2:10801161-10801180 Msa1233800:CDS 40.0%
!! GAAGTTGGTTCGGAACAATC+CGG - chr8_2:10798616-10798635 Msa1233800:three_prime_UTR 45.0%
CTAAAATCTGTTGGGCCACT+CGG - chr8_2:10798935-10798954 Msa1233800:CDS 45.0%
TAAAATCTGTTGGGCCACTC+GGG - chr8_2:10798936-10798955 Msa1233800:CDS 45.0%
TGCATCCAAGACTAGACATC+TGG + chr8_2:10798969-10798988 None:intergenic 45.0%
!! GATGTCTAGTCTTGGATGCA+AGG - chr8_2:10798969-10798988 Msa1233800:CDS 45.0%
!! TGTCTAGTCTTGGATGCAAG+GGG - chr8_2:10798971-10798990 Msa1233800:CDS 45.0%
GTCACACAATTGATGGGATG+TGG - chr8_2:10799006-10799025 Msa1233800:CDS 45.0%
!! GCTAAGTGTTGAACAAGCAG+AGG + chr8_2:10799383-10799402 None:intergenic 45.0%
!! AGAGTGTGGAAGGTATCATC+AGG - chr8_2:10799416-10799435 Msa1233800:intron 45.0%
TACTTTGCCTCCTTCTCAAC+TGG - chr8_2:10799462-10799481 Msa1233800:intron 45.0%
TTGTGATCCAGTTGAGAAGG+AGG + chr8_2:10799472-10799491 None:intergenic 45.0%
TGGTTGTGATCCAGTTGAGA+AGG + chr8_2:10799475-10799494 None:intergenic 45.0%
ACTAAAACGCTGTAGCGTAG+CGG + chr8_2:10799675-10799694 None:intergenic 45.0%
GAATGCAACATGAGCCTGAA+AGG + chr8_2:10799750-10799769 None:intergenic 45.0%
ATCCATATGGAGCTGCTAGT+AGG + chr8_2:10799799-10799818 None:intergenic 45.0%
TAGCAGCTCCATATGGATCA+CGG - chr8_2:10799801-10799820 Msa1233800:intron 45.0%
GTTAATCATGCTGAGCCAGT+AGG - chr8_2:10800338-10800357 Msa1233800:intron 45.0%
AAGAGGCAAAGGAATCCGAA+CGG + chr8_2:10800372-10800391 None:intergenic 45.0%
CTCTCTTCAATGCATGCAGA+TGG + chr8_2:10800611-10800630 None:intergenic 45.0%
CATGCATTGAAGAGAGCTAG+AGG - chr8_2:10800615-10800634 Msa1233800:intron 45.0%
ACCAAAAAGCTCCAAGACCA+TGG - chr8_2:10800660-10800679 Msa1233800:intron 45.0%
GATTCCAGCACATTTCCACT+CGG + chr8_2:10800717-10800736 None:intergenic 45.0%
GTGGAAATGTGCTGGAATCA+AGG - chr8_2:10800718-10800737 Msa1233800:intron 45.0%
GAATCCGACTTCAGATCATG+TGG - chr8_2:10800837-10800856 Msa1233800:intron 45.0%
TGGTTACCCTTCTCATATGC+AGG - chr8_2:10800857-10800876 Msa1233800:intron 45.0%
CTCACATAAGGACAGATAGG+CGG + chr8_2:10800926-10800945 None:intergenic 45.0%
TTCCATTGGGAAGTCATCCT+TGG - chr8_2:10800982-10801001 Msa1233800:intron 45.0%
! ATGAAGCACTCATGGCCATG+GGG - chr8_2:10798430-10798449 Msa1233800:three_prime_UTR 50.0%
GATTCAGAACAAGTCCCCCA+TGG + chr8_2:10798448-10798467 None:intergenic 50.0%
! TGGGGGACTTGTTCTGAATC+TGG - chr8_2:10798448-10798467 Msa1233800:three_prime_UTR 50.0%
ACGCTCCAGATGTCTAGTCT+TGG - chr8_2:10798961-10798980 Msa1233800:CDS 50.0%
TCAACACTTAGCGAAACCGG+CGG - chr8_2:10799391-10799410 Msa1233800:intron 50.0%
GGTATCATCAGGTCATCTGC+AGG - chr8_2:10799427-10799446 Msa1233800:intron 50.0%
CTGGCTCAGCATGATTAACC+TGG + chr8_2:10800337-10800356 None:intergenic 50.0%
AGAGGCAAAGGAATCCGAAC+GGG + chr8_2:10800371-10800390 None:intergenic 50.0%
TGGCGCGACTCATGTAGATA+TGG + chr8_2:10800591-10800610 None:intergenic 50.0%
TTGAAGAGAGCTAGAGGCTC+TGG - chr8_2:10800621-10800640 Msa1233800:intron 50.0%
!! GCCATGGTCTTGGAGCTTTT+TGG + chr8_2:10800664-10800683 None:intergenic 50.0%
GAATCCGAGTGGAAATGTGC+TGG - chr8_2:10800710-10800729 Msa1233800:intron 50.0%
ACTTGTGACGTCGGAACAAG+TGG + chr8_2:10800784-10800803 None:intergenic 50.0%
AGCCAAGGATGACTTCCCAA+TGG + chr8_2:10800987-10801006 None:intergenic 50.0%
! TGAAGCACTCATGGCCATGG+GGG - chr8_2:10798431-10798450 Msa1233800:three_prime_UTR 55.0%
ACCTTCCACACTCTTTCCGC+CGG + chr8_2:10799410-10799429 None:intergenic 55.0%
CAGGTCATCTGCAGGAAGTC+AGG - chr8_2:10799435-10799454 Msa1233800:intron 55.0%
GGCCTACTAGCAGCTCCATA+TGG - chr8_2:10799794-10799813 Msa1233800:intron 55.0%
CAGTAGGAATGCCTCCCGTT+CGG - chr8_2:10800354-10800373 Msa1233800:intron 55.0%
! AAGAGAGCTAGAGGCTCTGG+TGG - chr8_2:10800624-10800643 Msa1233800:intron 55.0%
ACTCGGATTCAGCCGAGTAG+TGG + chr8_2:10800700-10800719 None:intergenic 55.0%
ACTACTCGGCTGAATCCGAG+TGG - chr8_2:10800699-10800718 Msa1233800:intron 55.0%
GACGTCGGAACAAGTGGTTG+TGG + chr8_2:10800778-10800797 None:intergenic 55.0%
CAGGATTTGTCAGCCGATGC+CGG - chr8_2:10800876-10800895 Msa1233800:intron 55.0%
CGAAACCGGCGGAAAGAGTG+TGG - chr8_2:10799402-10799421 Msa1233800:intron 60.0%
! ACCGGCGGAAAGAGTGTGGA+AGG - chr8_2:10799406-10799425 Msa1233800:intron 60.0%
!! CGAACGGGAGGCATTCCTAC+TGG + chr8_2:10800356-10800375 None:intergenic 60.0%
GGCAAAGGAATCCGAACGGG+AGG + chr8_2:10800368-10800387 None:intergenic 60.0%
CGGATTCAGCCGAGTAGTGG+TGG + chr8_2:10800697-10800716 None:intergenic 60.0%
GATTTGTCAGCCGATGCCGG+TGG - chr8_2:10800879-10800898 Msa1233800:intron 60.0%
ATTCCGACCACCACCACCAC+CGG + chr8_2:10800898-10800917 None:intergenic 60.0%
! CATCTGGAGCGTGACCCGAG+TGG + chr8_2:10798953-10798972 None:intergenic 65.0%
TTGTCAGCCGATGCCGGTGG+TGG - chr8_2:10800882-10800901 Msa1233800:intron 65.0%
ACCACCACCACCACCGGCAT+CGG + chr8_2:10800892-10800911 None:intergenic 65.0%
!! ATGCCGGTGGTGGTGGTGGT+CGG - chr8_2:10800892-10800911 Msa1233800:intron 65.0%
! TCAGCCGATGCCGGTGGTGG+TGG - chr8_2:10800885-10800904 Msa1233800:intron 70.0%
!! GCCGATGCCGGTGGTGGTGG+TGG - chr8_2:10800888-10800907 Msa1233800:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 10798430 10801225 10798430 ID=Msa1233800;Name=Msa1233800
chr8_2 mRNA 10798430 10801225 10798430 ID=Msa1233800-mRNA-1;Parent=Msa1233800;Name=Msa1233800-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.75|0.6|1|1|1|5|281|318
chr8_2 exon 10798430 10799067 10798430 ID=Msa1233800-mRNA-1:exon:4218;Parent=Msa1233800-mRNA-1
chr8_2 exon 10799150 10799317 10799150 ID=Msa1233800-mRNA-1:exon:4217;Parent=Msa1233800-mRNA-1
chr8_2 exon 10799826 10799900 10799826 ID=Msa1233800-mRNA-1:exon:4216;Parent=Msa1233800-mRNA-1
chr8_2 exon 10800154 10800302 10800154 ID=Msa1233800-mRNA-1:exon:4215;Parent=Msa1233800-mRNA-1
chr8_2 exon 10801018 10801225 10801018 ID=Msa1233800-mRNA-1:exon:4214;Parent=Msa1233800-mRNA-1
chr8_2 CDS 10801018 10801225 10801018 ID=Msa1233800-mRNA-1:cds;Parent=Msa1233800-mRNA-1
chr8_2 CDS 10800154 10800302 10800154 ID=Msa1233800-mRNA-1:cds;Parent=Msa1233800-mRNA-1
chr8_2 CDS 10799826 10799900 10799826 ID=Msa1233800-mRNA-1:cds;Parent=Msa1233800-mRNA-1
chr8_2 CDS 10799150 10799317 10799150 ID=Msa1233800-mRNA-1:cds;Parent=Msa1233800-mRNA-1
chr8_2 CDS 10798711 10799067 10798711 ID=Msa1233800-mRNA-1:cds;Parent=Msa1233800-mRNA-1
chr8_2 three_prime_UTR 10798430 10798710 10798430 ID=Msa1233800-mRNA-1:three_prime_utr;Parent=Msa1233800-mRNA-1
Gene Sequence

>Msa1233800

ATGAAGCACTCATGGCCATGGGGGACTTGTTCTGAATCTGGTGTGCAACAATCCTCTATGTCAGAAAATCTAACTTTGAATATGAGTGTTTTGCAACAAGAATGCAACAAGTCTAAACCACTAGGTTTTCAATTTCAAGACCAAGATTCTTCTTCTACTCAATCAACTGGTGGTCAATCTTATCTGGAAGTTGGTTCGGAACAATCCGGTCCAATTTCTGTCCAGTATAACAATTCCTCTGCTTGTTCAACACTTAGCGAAACCGGCGGAAAGAGTGTGGAAGGTATCATCAGGTCATCTGCAGGAAGTCAGGATTTTACTTTGCCTCCTTCTCAACTGGATCACAACCAATCACTTGCTCATGTTGCATTCCCTCATACTGAGACATACTCTAATGGCCTACTAGCAGCTCCATATGGATCACGGAATAATGTTAATCATGCTGAGCCAGTAGGAATGCCTCCCGTTCGGATTCCTTTGCCTCTTAATCTTTGTGAAGAACCAATATACGTGAATGCAAAGCAGTACCATGCTATTATGAGACGTAGACAGTATCGTGCAAAGCTCGAAGCACAGAACAAACTCGTCAAAAATCGGAAACCATATCTACATGAGTCGCGCCATCTGCATGCATTGAAGAGAGCTAGAGGCTCTGGTGGACGTTTTCTAAATACCAAAAAGCTCCAAGACCATGGCTTTAATGTTTCCACCACTACTCGGCTGAATCCGAGTGGAAATGTGCTGGAATCAAGGGTACATCAAGTAGAAAAGTATAGAGATGGTGCTTCCACAACCACTTGTTCCGACGTCACAAGTGCTTCTAATAGTGACGACATGTTCCAACAACATGAATCCGACTTCAGATCATGTGGTTACCCTTCTCATATGCAGGATTTGTCAGCCGATGCCGGTGGTGGTGGTGGTCGGAATCAACACCGCCTATCTGTCCTTATGTGA
Protein sequence

>Msa1233800

MKHSWPWGTCSESGVQQSSMSENLTLNMSVLQQECNKSKPLGFQFQDQDSSSTQSTGGQSYLEVGSEQSGPISVQYNNSSACSTLSETGGKSVEGIIRSSAGSQDFTLPPSQLDHNQSLAHVAFPHTETYSNGLLAAPYGSRNNVNHAEPVGMPPVRIPLPLNLCEEPIYVNAKQYHAIMRRRQYRAKLEAQNKLVKNRKPYLHESRHLHALKRARGSGGRFLNTKKLQDHGFNVSTTTRLNPSGNVLESRVHQVEKYRDGASTTTCSDVTSASNSDDMFQQHESDFRSCGYPSHMQDLSADAGGGGGRNQHRLSVLM*