AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1314300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1314300 MtrunA17_Chr8g0343451 91.875 320 22 3 1 318 1 318 0.0 561
Msa1314300 MtrunA17_Chr2g0329811 50.737 339 124 10 7 318 23 345 2.10e-86 263
Msa1314300 MtrunA17_Chr2g0300261 48.404 188 86 6 111 287 17 204 2.97e-45 154
Msa1314300 MtrunA17_Chr2g0292411 62.222 90 33 1 138 227 85 173 6.04e-33 120
Msa1314300 MtrunA17_Chr3g0103391 53.774 106 45 2 118 222 124 226 6.54e-32 121
Msa1314300 MtrunA17_Chr8g0352251 44.444 171 73 7 130 280 115 283 5.51e-31 118
Msa1314300 MtrunA17_Chr7g0267581 47.500 120 44 4 112 227 122 226 4.20e-26 105
Msa1314300 MtrunA17_Chr1g0177091 47.899 119 43 4 112 226 120 223 4.35e-26 105
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1314300 AT3G14020.2 45.130 308 128 14 4 294 17 300 2.26e-57 187
Msa1314300 AT3G14020.1 45.130 308 128 14 4 294 17 300 2.26e-57 187
Msa1314300 AT1G72830.1 37.576 330 175 9 4 316 23 338 4.02e-51 172
Msa1314300 AT1G72830.2 37.462 331 175 10 4 316 23 339 2.82e-49 167
Msa1314300 AT1G54160.1 48.889 225 97 7 17 230 24 241 5.04e-48 163
Msa1314300 AT1G17590.3 37.920 327 161 12 7 316 25 326 8.39e-47 160
Msa1314300 AT1G17590.2 37.920 327 161 12 7 316 25 326 8.39e-47 160
Msa1314300 AT1G17590.4 37.920 327 161 12 7 316 25 326 8.39e-47 160
Msa1314300 AT1G17590.1 37.920 327 161 12 7 316 25 326 8.39e-47 160
Msa1314300 AT1G72830.3 34.043 329 163 9 4 316 23 313 1.89e-37 136
Msa1314300 AT1G30500.2 52.632 114 53 1 118 231 52 164 1.59e-35 127
Msa1314300 AT1G30500.1 50.877 114 51 1 118 231 52 160 2.78e-34 124
Msa1314300 AT2G34720.1 47.059 119 53 2 115 227 44 158 5.62e-31 115
Msa1314300 AT5G12840.4 46.032 126 64 3 104 229 112 233 2.29e-30 116
Msa1314300 AT5G12840.2 46.032 126 64 3 104 229 112 233 2.29e-30 116
Msa1314300 AT5G12840.5 46.032 126 64 3 104 229 113 234 2.62e-30 116
Msa1314300 AT5G12840.3 46.032 126 64 3 104 229 113 234 2.62e-30 116
Msa1314300 AT5G12840.1 46.032 126 64 3 104 229 113 234 2.62e-30 116
Msa1314300 AT2G34720.2 49.123 114 48 2 120 227 14 123 6.70e-30 112
Msa1314300 AT2G34720.3 49.123 114 48 2 120 227 14 123 6.70e-30 112
Msa1314300 AT3G20910.1 45.802 131 43 4 106 222 105 221 3.44e-29 114
Msa1314300 AT5G06510.1 51.351 111 40 5 117 226 93 190 2.02e-24 100
Msa1314300 AT5G06510.5 51.351 111 40 5 117 226 93 190 2.02e-24 100
Msa1314300 AT5G06510.2 51.351 111 40 5 117 226 93 190 2.02e-24 100
Msa1314300 AT5G06510.6 51.351 111 40 5 117 226 93 190 2.02e-24 100
Msa1314300 AT3G05690.1 46.667 135 50 6 109 235 90 210 3.54e-24 100
Msa1314300 AT5G06510.3 51.351 111 40 5 117 226 44 141 3.86e-24 98.6
Msa1314300 AT5G06510.4 54.369 103 33 5 125 226 6 95 7.75e-24 96.3
Msa1314300 AT5G06510.7 54.369 103 33 5 125 226 6 95 7.75e-24 96.3
Msa1314300 AT3G05690.2 46.667 135 50 6 109 235 127 247 8.84e-24 99.8

Find 89 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTATTATTCAAGAATA+AGG 0.217766 8_4:-13214315 Msa1314300:three_prime_UTR
TTGTTATACTGGACAGAAAT+TGG 0.227693 8_4:+13216019 None:intergenic
TACTTTGCCTCCTTCTCAAC+TGG 0.301003 8_4:-13215912 Msa1314300:CDS
AACTGGTGGTCAATCTTATC+TGG 0.313777 8_4:-13216780 Msa1314300:CDS
TGCTTTGCATTCACGTATAT+TGG 0.351246 8_4:+13214947 None:intergenic
ACATTATTCCGTGATCCATA+TGG 0.396988 8_4:+13215565 None:intergenic
CAATGCTTGTTGCATTCCAT+TGG 0.402633 8_4:-13214386 Msa1314300:three_prime_UTR
CATATATACTTACAAGTGAT+TGG 0.407417 8_4:+13215882 None:intergenic
ATCTTATCTGGAAGTTGGTT+CGG 0.412123 8_4:-13216768 Msa1314300:CDS
GGTTCTTCACAAAGATTAAG+AGG 0.415954 8_4:+13214968 None:intergenic
CAGGTCATCTGCAGGAAGTC+AGG 0.433122 8_4:-13215939 Msa1314300:CDS
TTTGTAGCCATGAAAATGTT+TGG 0.434600 8_4:-13214264 Msa1314300:three_prime_UTR
TTCCATTGGGAAGTCATCCT+TGG 0.442295 8_4:-13214372 Msa1314300:three_prime_UTR
TGAAGTCGGATTCATGTTGT+TGG 0.453276 8_4:+13214527 None:intergenic
ATTGAGTAGAAGAAGAATCT+TGG 0.456059 8_4:+13216804 None:intergenic
AAACATTAAAGCCATGGTCT+TGG 0.460398 8_4:+13214683 None:intergenic
TGTTCAACACTTAACGAAAC+CGG 0.461471 8_4:-13215986 Msa1314300:CDS
CATGCTGAGACATACTCTAA+TGG 0.462998 8_4:-13215601 Msa1314300:CDS
AAGCAGAGGAATTGTTATAC+TGG 0.467814 8_4:+13216008 None:intergenic
GGCCTACTAGCAGCTCCATA+TGG 0.471924 8_4:-13215580 Msa1314300:CDS
TCTTCTTCTACTCAATCAAC+TGG 0.473460 8_4:-13216797 Msa1314300:CDS
GTGGAAATGTGCTGGAATCA+AGG 0.474923 8_4:-13214636 Msa1314300:CDS
TGGTTACCCTTCTCATATGC+AGG 0.475880 8_4:-13214497 Msa1314300:CDS
CTAATCATGTCTCTCACATA+AGG 0.480287 8_4:+13214419 None:intergenic
TTGAAGAGAGCTAGAGGCTC+TGG 0.481702 8_4:-13214733 Msa1314300:CDS
GCCGATGCCGGTGGTGGTGG+TGG 0.483222 8_4:-13214466 Msa1314300:CDS
TGGCGCGACTCATGTAGATA+TGG 0.485664 8_4:+13214766 None:intergenic
TCAGCCGATGCCGGTGGTGG+TGG 0.489227 8_4:-13214469 Msa1314300:CDS
CGAACGGGAGGCATTCCTAC+TGG 0.492795 8_4:+13215001 None:intergenic
CAATGAAGCACTCATGGCCA+TGG 0.498100 8_4:-13216946 Msa1314300:CDS
GGTCAATCTTATCTGGAAGT+TGG 0.499258 8_4:-13216773 Msa1314300:CDS
CAGTAGGAATGCCTCCCGTT+CGG 0.506402 8_4:-13215000 Msa1314300:CDS
TTGTCAGCCGATGCCGGTGG+TGG 0.509405 8_4:-13214472 Msa1314300:CDS
AATGCTTGTTGCATTCCATT+GGG 0.516081 8_4:-13214385 Msa1314300:three_prime_UTR
ATTCCGACCACCACCACCAC+CGG 0.525135 8_4:+13214459 None:intergenic
CAGGATTTGTCAGCCGATGC+CGG 0.529122 8_4:-13214478 Msa1314300:CDS
TTAATGTTTCCACCACTACT+CGG 0.534943 8_4:-13214669 Msa1314300:CDS
TCTCTCACATAAGGACAGAT+AGG 0.538450 8_4:+13214428 None:intergenic
ACCTTCCACACTCTTTCCGC+CGG 0.539251 8_4:+13215967 None:intergenic
TCACAAAGATTAAGAGGCAA+AGG 0.539813 8_4:+13214974 None:intergenic
AGAGGCAAAGGAATCCGAAC+GGG 0.545487 8_4:+13214986 None:intergenic
ATGGCTTGTTGAAAATCAAA+TGG 0.546725 8_4:-13214174 Msa1314300:three_prime_UTR
TGGAAATGTGCTGGAATCAA+GGG 0.553488 8_4:-13214635 Msa1314300:CDS
GAAGTTGGTTCGGAACAATC+CGG 0.553531 8_4:-13216758 Msa1314300:intron
AGAGTGTGGAAGGTATCATC+AGG 0.556743 8_4:-13215958 Msa1314300:CDS
GACAAATCCTGCATATGAGA+AGG 0.557039 8_4:+13214490 None:intergenic
AATGAAGCACTCATGGCCAT+GGG 0.561056 8_4:-13216945 Msa1314300:CDS
GGAAAACAATGAAGCACTCA+TGG 0.561079 8_4:-13216952 None:intergenic
CGAAACCGGCGGAAAGAGTG+TGG 0.561166 8_4:-13215972 Msa1314300:CDS
ATCCATATGGAGCTGCTAGT+AGG 0.561398 8_4:+13215578 None:intergenic
GAATCCGAGTGGAAATGTGC+TGG 0.564823 8_4:-13214644 Msa1314300:CDS
CTCTCTTCAATGCATGCAGA+TGG 0.566697 8_4:+13214746 None:intergenic
CGGATTCAGCCGAGTAGTGG+TGG 0.574639 8_4:+13214660 None:intergenic
GGTATCATCAGGTCATCTGC+AGG 0.575460 8_4:-13215947 Msa1314300:CDS
ACTCGGATTCAGCCGAGTAG+TGG 0.577382 8_4:+13214657 None:intergenic
ACTTGTGACGTCGGAACAAG+TGG 0.585137 8_4:+13214573 None:intergenic
TTAGAGTATGTCTCAGCATG+AGG 0.588380 8_4:+13215604 None:intergenic
TCAAATGGTGTTGAATGTGT+AGG 0.593408 8_4:-13214159 Msa1314300:three_prime_UTR
CAAGTAGAAAAGTATAGAGA+TGG 0.594749 8_4:-13214607 Msa1314300:CDS
TCTTCTACTCAATCAACTGG+TGG 0.595236 8_4:-13216794 Msa1314300:CDS
GTTAAGTGTTGAACAAGCAG+AGG 0.603154 8_4:+13215994 None:intergenic
ACCGGCGGAAAGAGTGTGGA+AGG 0.603635 8_4:-13215968 Msa1314300:CDS
TTGTGATCCAGTTGAGAAGG+AGG 0.605135 8_4:+13215905 None:intergenic
GTTAATCATGCTGAGCCAGT+AGG 0.605706 8_4:-13215016 Msa1314300:CDS
TGGTGGAAACATTAAAGCCA+TGG 0.608658 8_4:+13214677 None:intergenic
GAATCCGACTTCAGATCAAG+TGG 0.610066 8_4:-13214517 Msa1314300:CDS
AAGAGAGCTAGAGGCTCTGG+TGG 0.614598 8_4:-13214730 Msa1314300:CDS
CATGCATTGAAGAGAGCTAG+AGG 0.616928 8_4:-13214739 Msa1314300:CDS
ACATAATTCAAAATGAGCCA+AGG 0.618165 8_4:+13214355 None:intergenic
GATTCCAGCACATTTCCACT+CGG 0.619314 8_4:+13214640 None:intergenic
TCTTGAAATTGAAAACCTAG+TGG 0.627398 8_4:+13216827 None:intergenic
ACAAATCCTGCATATGAGAA+GGG 0.629662 8_4:+13214491 None:intergenic
GATTTGTCAGCCGATGCCGG+TGG 0.632484 8_4:-13214475 Msa1314300:CDS
TGGTTGTGATCCAGTTGAGA+AGG 0.633407 8_4:+13215902 None:intergenic
TAGCAGCTCCATATGGATCA+CGG 0.633412 8_4:-13215573 Msa1314300:CDS
GGCAAAGGAATCCGAACGGG+AGG 0.635841 8_4:+13214989 None:intergenic
AGCCAAGGATGACTTCCCAA+TGG 0.637029 8_4:+13214370 None:intergenic
TAGAGTATGTCTCAGCATGA+GGG 0.637829 8_4:+13215605 None:intergenic
ATTAGAAGCACTTGTGACGT+CGG 0.640775 8_4:+13214564 None:intergenic
GTCTACGTCTCATAATAGCA+TGG 0.645173 8_4:+13214921 None:intergenic
GTAACCACTTGATCTGAAGT+CGG 0.645350 8_4:+13214513 None:intergenic
AAGAGGCAAAGGAATCCGAA+CGG 0.660223 8_4:+13214985 None:intergenic
GACGTCGGAACAAGTGGTTG+TGG 0.668249 8_4:+13214579 None:intergenic
TGAAGCACTCATGGCCATGG+GGG 0.672419 8_4:-13216943 Msa1314300:CDS
TGCAACAAGTCTAAACCACT+AGG 0.672944 8_4:-13216842 Msa1314300:CDS
ATGAAGCACTCATGGCCATG+GGG 0.681773 8_4:-13216944 Msa1314300:CDS
TCAACACTTAACGAAACCGG+CGG 0.689738 8_4:-13215983 Msa1314300:CDS
ACTACTCGGCTGAATCCGAG+TGG 0.691698 8_4:-13214655 Msa1314300:CDS
CTCACATAAGGACAGATAGG+CGG 0.718305 8_4:+13214431 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTAAGTTTTTTCCTATA+CGG + chr8_4:13214961-13214980 None:intergenic 15.0%
!! ATTTAATTACGAATAGTTAC+CGG + chr8_4:13214359-13214378 None:intergenic 20.0%
!!! AAATTCTATCTAAAATCTGT+TGG - chr8_4:13214647-13214666 Msa1314300:CDS 20.0%
!!! AATTCTATCTAAAATCTGTT+GGG - chr8_4:13214648-13214667 Msa1314300:CDS 20.0%
!! AATATTTGCAAGAAAAACTA+TGG - chr8_4:13215596-13215615 Msa1314300:CDS 20.0%
!! ATCATATTTATGTCTTTATC+TGG - chr8_4:13215620-13215639 Msa1314300:CDS 20.0%
!! TGTATGTATATTTCAGAATT+CGG + chr8_4:13216039-13216058 None:intergenic 20.0%
!! CTTCTTATTATTCAAGAATA+AGG - chr8_4:13216780-13216799 Msa1314300:CDS 20.0%
! AAAGTAGATTATTAGAGATG+TGG - chr8_4:13214441-13214460 Msa1314300:CDS 25.0%
! GAATTACATCAAGTCTAAAT+AGG + chr8_4:13214504-13214523 None:intergenic 25.0%
! CTGAAAATGAGTTTATAAGT+GGG - chr8_4:13214751-13214770 Msa1314300:CDS 25.0%
! AATGAAGTGATACAAATTTG+TGG - chr8_4:13214887-13214906 Msa1314300:CDS 25.0%
! AATACTCATCAATATGCATT+AGG + chr8_4:13215046-13215065 None:intergenic 25.0%
! CATATATACTTACAAGTGAT+TGG + chr8_4:13215216-13215235 None:intergenic 25.0%
!!! TACAAAGTGTTGATAAATAG+AGG + chr8_4:13215332-13215351 None:intergenic 25.0%
!!! TTAATTTGTGATACGCTATT+TGG + chr8_4:13215355-13215374 None:intergenic 25.0%
!!! TTGCAAATATTTTTGACACA+AGG + chr8_4:13215587-13215606 None:intergenic 25.0%
!! TTGTATGTTTCTGATATTTG+AGG - chr8_4:13215651-13215670 Msa1314300:intron 25.0%
! AGAATTCGTTCAAATTCAAA+AGG + chr8_4:13215832-13215851 None:intergenic 25.0%
!! GAAGTGTTTATAGATTCTAT+TGG - chr8_4:13215946-13215965 Msa1314300:CDS 25.0%
! TGTTTATAGATTCTATTGGT+TGG - chr8_4:13215950-13215969 Msa1314300:CDS 25.0%
!!! ATCAAGCTATATTTTTGTCT+AGG - chr8_4:13216890-13216909 Msa1314300:CDS 25.0%
! AGTTAGATTTTCTGACATAG+AGG + chr8_4:13214206-13214225 None:intergenic 30.0%
TCTTGAAATTGAAAACCTAG+TGG + chr8_4:13214271-13214290 None:intergenic 30.0%
ATTGAGTAGAAGAAGAATCT+TGG + chr8_4:13214294-13214313 None:intergenic 30.0%
TTAAGAGTCACACAATTGAT+GGG - chr8_4:13214721-13214740 Msa1314300:CDS 30.0%
! CCACTTATAAACTCATTTTC+AGG + chr8_4:13214753-13214772 None:intergenic 30.0%
CCTGAAAATGAGTTTATAAG+TGG - chr8_4:13214750-13214769 Msa1314300:CDS 30.0%
TAATTCAACCCCTACAAAAT+AGG + chr8_4:13214798-13214817 None:intergenic 30.0%
! TATTTTGTAGGGGTTGAATT+AGG - chr8_4:13214797-13214816 Msa1314300:intron 30.0%
TGAGTACTCTCTTTGAATTT+GGG + chr8_4:13214828-13214847 None:intergenic 30.0%
AACTAGTATTAGAAAGCTGT+TGG - chr8_4:13214993-13215012 Msa1314300:CDS 30.0%
TATTGATGAGTATTTGCTCT+AGG - chr8_4:13215052-13215071 Msa1314300:intron 30.0%
TTGTTATACTGGACAGAAAT+TGG + chr8_4:13215079-13215098 None:intergenic 30.0%
GTATATATGTGTTTGTGTGT+TGG - chr8_4:13215227-13215246 Msa1314300:intron 30.0%
! GTAGCGGAATTTGAATAAAT+TGG + chr8_4:13215380-13215399 None:intergenic 30.0%
! TTTCTTATAGTTTGCCTTTC+AGG - chr8_4:13215454-13215473 Msa1314300:intron 30.0%
AACTTAACACATACATAAGG+CGG + chr8_4:13215696-13215715 None:intergenic 30.0%
CCAAACTTAACACATACATA+AGG + chr8_4:13215699-13215718 None:intergenic 30.0%
CCTTATGTATGTGTTAAGTT+TGG - chr8_4:13215696-13215715 Msa1314300:intron 30.0%
AAAAAGAGAAACACCTTTGT+AGG - chr8_4:13215778-13215797 Msa1314300:intron 30.0%
! AATAGCATGTTTTCCTACAA+AGG + chr8_4:13215794-13215813 None:intergenic 30.0%
! GCTAAGTAAAAGGCATAAAT+AGG + chr8_4:13215920-13215939 None:intergenic 30.0%
! CTTCTAAGAAGCTAAGTAAA+AGG + chr8_4:13215930-13215949 None:intergenic 30.0%
AGAACAAACTCGTCAAAAAT+CGG - chr8_4:13216221-13216240 Msa1314300:intron 30.0%
! TTTCTGTTTCCTTTTCTCTA+AGG - chr8_4:13216257-13216276 Msa1314300:intron 30.0%
! TTCTGTTTCCTTTTCTCTAA+GGG - chr8_4:13216258-13216277 Msa1314300:intron 30.0%
ATAAATCTCCCTTAGAGAAA+AGG + chr8_4:13216269-13216288 None:intergenic 30.0%
! CAAGTAGAAAAGTATAGAGA+TGG - chr8_4:13216488-13216507 Msa1314300:intron 30.0%
ACATAATTCAAAATGAGCCA+AGG + chr8_4:13216743-13216762 None:intergenic 30.0%
TTTGTAGCCATGAAAATGTT+TGG - chr8_4:13216831-13216850 Msa1314300:CDS 30.0%
! TCTTTAGCCAAACATTTTCA+TGG + chr8_4:13216841-13216860 None:intergenic 30.0%
! TTGATTTTCAACAAGCCATT+TGG + chr8_4:13216920-13216939 None:intergenic 30.0%
!! ATGGCTTGTTGAAAATCAAA+TGG - chr8_4:13216921-13216940 Msa1314300:CDS 30.0%
TCTTCTTCTACTCAATCAAC+TGG - chr8_4:13214298-13214317 Msa1314300:three_prime_UTR 35.0%
! ATCTTATCTGGAAGTTGGTT+CGG - chr8_4:13214327-13214346 Msa1314300:three_prime_UTR 35.0%
ACAAGAGTCAAATGCAGATA+TGG + chr8_4:13214387-13214406 None:intergenic 35.0%
AGAAAACATGATATCCACGA+AGG + chr8_4:13214621-13214640 None:intergenic 35.0%
GTTAAGAGTCACACAATTGA+TGG - chr8_4:13214720-13214739 Msa1314300:CDS 35.0%
! ACCTTACAAGCCTATTTTGT+AGG - chr8_4:13214785-13214804 Msa1314300:intron 35.0%
! CCTTACAAGCCTATTTTGTA+GGG - chr8_4:13214786-13214805 Msa1314300:intron 35.0%
! CTTACAAGCCTATTTTGTAG+GGG - chr8_4:13214787-13214806 Msa1314300:intron 35.0%
GTGAGTACTCTCTTTGAATT+TGG + chr8_4:13214829-13214848 None:intergenic 35.0%
AAGCAGAGGAATTGTTATAC+TGG + chr8_4:13215090-13215109 None:intergenic 35.0%
TGTTCAACACTTAACGAAAC+CGG - chr8_4:13215109-13215128 Msa1314300:intron 35.0%
! ACAGTAAGTGTTGTCAATTG+TGG - chr8_4:13215260-13215279 Msa1314300:intron 35.0%
ACATTATTCCGTGATCCATA+TGG + chr8_4:13215533-13215552 None:intergenic 35.0%
GTTCGTTTGAGTAAATGTTG+CGG - chr8_4:13215885-13215904 Msa1314300:intron 35.0%
TCAAGTAACTCAATGGCATT+TGG + chr8_4:13215990-13216009 None:intergenic 35.0%
TGGTACATCAAGTAACTCAA+TGG + chr8_4:13215997-13216016 None:intergenic 35.0%
TTGAGTTACTTGATGTACCA+TGG - chr8_4:13215997-13216016 Msa1314300:CDS 35.0%
CATGCTATAAAAATCTTGCC+AGG - chr8_4:13216057-13216076 Msa1314300:intron 35.0%
TCACAAAGATTAAGAGGCAA+AGG + chr8_4:13216124-13216143 None:intergenic 35.0%
GGTTCTTCACAAAGATTAAG+AGG + chr8_4:13216130-13216149 None:intergenic 35.0%
TGCTTTGCATTCACGTATAT+TGG + chr8_4:13216151-13216170 None:intergenic 35.0%
AAACATTAAAGCCATGGTCT+TGG + chr8_4:13216415-13216434 None:intergenic 35.0%
TTAATGTTTCCACCACTACT+CGG - chr8_4:13216426-13216445 Msa1314300:intron 35.0%
ACAAATCCTGCATATGAGAA+GGG + chr8_4:13216607-13216626 None:intergenic 35.0%
CTAATCATGTCTCTCACATA+AGG + chr8_4:13216679-13216698 None:intergenic 35.0%
! AATGCTTGTTGCATTCCATT+GGG - chr8_4:13216710-13216729 Msa1314300:intron 35.0%
! TCAAATGGTGTTGAATGTGT+AGG - chr8_4:13216936-13216955 Msa1314300:CDS 35.0%
TGCAACAAGTCTAAACCACT+AGG - chr8_4:13214253-13214272 Msa1314300:three_prime_UTR 40.0%
TCTTCTACTCAATCAACTGG+TGG - chr8_4:13214301-13214320 Msa1314300:three_prime_UTR 40.0%
AACTGGTGGTCAATCTTATC+TGG - chr8_4:13214315-13214334 Msa1314300:three_prime_UTR 40.0%
GGTCAATCTTATCTGGAAGT+TGG - chr8_4:13214322-13214341 Msa1314300:three_prime_UTR 40.0%
! TTTTCTCTCTTCTACCTTCG+TGG - chr8_4:13214604-13214623 Msa1314300:CDS 40.0%
!! ATGTCTAGTCTTGGATGCAA+GGG - chr8_4:13214691-13214710 Msa1314300:CDS 40.0%
CCCTACAAAATAGGCTTGTA+AGG + chr8_4:13214789-13214808 None:intergenic 40.0%
CTCTGCATTGAATACCGTAT+AGG - chr8_4:13214944-13214963 Msa1314300:CDS 40.0%
!! GTTAAGTGTTGAACAAGCAG+AGG + chr8_4:13215104-13215123 None:intergenic 40.0%
AGCGACACTATAACATTGGA+AGG + chr8_4:13215305-13215324 None:intergenic 40.0%
CGATAGCGACACTATAACAT+TGG + chr8_4:13215309-13215328 None:intergenic 40.0%
TAGAGTATGTCTCAGCATGA+GGG + chr8_4:13215493-13215512 None:intergenic 40.0%
TTAGAGTATGTCTCAGCATG+AGG + chr8_4:13215494-13215513 None:intergenic 40.0%
CATGCTGAGACATACTCTAA+TGG - chr8_4:13215494-13215513 Msa1314300:intron 40.0%
GTGATAGACTGAATTCACCA+TGG + chr8_4:13216017-13216036 None:intergenic 40.0%
GTCTACGTCTCATAATAGCA+TGG + chr8_4:13216177-13216196 None:intergenic 40.0%
TGGTGGAAACATTAAAGCCA+TGG + chr8_4:13216421-13216440 None:intergenic 40.0%
TGGAAATGTGCTGGAATCAA+GGG - chr8_4:13216460-13216479 Msa1314300:intron 40.0%
!! ATTAGAAGCACTTGTGACGT+CGG + chr8_4:13216534-13216553 None:intergenic 40.0%
TGAAGTCGGATTCATGTTGT+TGG + chr8_4:13216571-13216590 None:intergenic 40.0%
! GTAACCACTTGATCTGAAGT+CGG + chr8_4:13216585-13216604 None:intergenic 40.0%
GACAAATCCTGCATATGAGA+AGG + chr8_4:13216608-13216627 None:intergenic 40.0%
TCTCTCACATAAGGACAGAT+AGG + chr8_4:13216670-13216689 None:intergenic 40.0%
! CAATGCTTGTTGCATTCCAT+TGG - chr8_4:13216709-13216728 Msa1314300:intron 40.0%
!! TTTTGTCTAGGACTGCCAAA+TGG - chr8_4:13216902-13216921 Msa1314300:CDS 40.0%
!! GAAGTTGGTTCGGAACAATC+CGG - chr8_4:13214337-13214356 Msa1314300:three_prime_UTR 45.0%
CTAAAATCTGTTGGGCCACT+CGG - chr8_4:13214656-13214675 Msa1314300:CDS 45.0%
TAAAATCTGTTGGGCCACTC+GGG - chr8_4:13214657-13214676 Msa1314300:CDS 45.0%
TGCATCCAAGACTAGACATC+TGG + chr8_4:13214690-13214709 None:intergenic 45.0%
!! GATGTCTAGTCTTGGATGCA+AGG - chr8_4:13214690-13214709 Msa1314300:CDS 45.0%
!! TGTCTAGTCTTGGATGCAAG+GGG - chr8_4:13214692-13214711 Msa1314300:CDS 45.0%
GTCACACAATTGATGGGATG+TGG - chr8_4:13214727-13214746 Msa1314300:CDS 45.0%
TCAACACTTAACGAAACCGG+CGG - chr8_4:13215112-13215131 Msa1314300:intron 45.0%
!! AGAGTGTGGAAGGTATCATC+AGG - chr8_4:13215137-13215156 Msa1314300:intron 45.0%
TACTTTGCCTCCTTCTCAAC+TGG - chr8_4:13215183-13215202 Msa1314300:intron 45.0%
TTGTGATCCAGTTGAGAAGG+AGG + chr8_4:13215193-13215212 None:intergenic 45.0%
TGGTTGTGATCCAGTTGAGA+AGG + chr8_4:13215196-13215215 None:intergenic 45.0%
ACTAAAACGCTGTAGCGTAG+CGG + chr8_4:13215396-13215415 None:intergenic 45.0%
GAATGCAACATGAGCCTGAA+AGG + chr8_4:13215471-13215490 None:intergenic 45.0%
ATCCATATGGAGCTGCTAGT+AGG + chr8_4:13215520-13215539 None:intergenic 45.0%
TAGCAGCTCCATATGGATCA+CGG - chr8_4:13215522-13215541 Msa1314300:intron 45.0%
CGTTTGAGTAAATGTTGCGG+TGG - chr8_4:13215888-13215907 Msa1314300:intron 45.0%
GTTAATCATGCTGAGCCAGT+AGG - chr8_4:13216079-13216098 Msa1314300:intron 45.0%
AAGAGGCAAAGGAATCCGAA+CGG + chr8_4:13216113-13216132 None:intergenic 45.0%
CTCTCTTCAATGCATGCAGA+TGG + chr8_4:13216352-13216371 None:intergenic 45.0%
CATGCATTGAAGAGAGCTAG+AGG - chr8_4:13216356-13216375 Msa1314300:intron 45.0%
ACCAAAAAGCTCCAAGACCA+TGG - chr8_4:13216401-13216420 Msa1314300:intron 45.0%
GATTCCAGCACATTTCCACT+CGG + chr8_4:13216458-13216477 None:intergenic 45.0%
GTGGAAATGTGCTGGAATCA+AGG - chr8_4:13216459-13216478 Msa1314300:intron 45.0%
GAATCCGACTTCAGATCAAG+TGG - chr8_4:13216578-13216597 Msa1314300:intron 45.0%
TGGTTACCCTTCTCATATGC+AGG - chr8_4:13216598-13216617 Msa1314300:intron 45.0%
CTCACATAAGGACAGATAGG+CGG + chr8_4:13216667-13216686 None:intergenic 45.0%
TTCCATTGGGAAGTCATCCT+TGG - chr8_4:13216723-13216742 Msa1314300:intron 45.0%
! ATGAAGCACTCATGGCCATG+GGG - chr8_4:13214151-13214170 Msa1314300:three_prime_UTR 50.0%
GATTCAGAACAAGTCCCCCA+TGG + chr8_4:13214169-13214188 None:intergenic 50.0%
! TGGGGGACTTGTTCTGAATC+TGG - chr8_4:13214169-13214188 Msa1314300:three_prime_UTR 50.0%
ACGCTCCAGATGTCTAGTCT+TGG - chr8_4:13214682-13214701 Msa1314300:CDS 50.0%
GGTATCATCAGGTCATCTGC+AGG - chr8_4:13215148-13215167 Msa1314300:intron 50.0%
CTGGCTCAGCATGATTAACC+TGG + chr8_4:13216078-13216097 None:intergenic 50.0%
AGAGGCAAAGGAATCCGAAC+GGG + chr8_4:13216112-13216131 None:intergenic 50.0%
TGGCGCGACTCATGTAGATA+TGG + chr8_4:13216332-13216351 None:intergenic 50.0%
TTGAAGAGAGCTAGAGGCTC+TGG - chr8_4:13216362-13216381 Msa1314300:intron 50.0%
!! GCCATGGTCTTGGAGCTTTT+TGG + chr8_4:13216405-13216424 None:intergenic 50.0%
GAATCCGAGTGGAAATGTGC+TGG - chr8_4:13216451-13216470 Msa1314300:intron 50.0%
ACTTGTGACGTCGGAACAAG+TGG + chr8_4:13216525-13216544 None:intergenic 50.0%
AGCCAAGGATGACTTCCCAA+TGG + chr8_4:13216728-13216747 None:intergenic 50.0%
! TGAAGCACTCATGGCCATGG+GGG - chr8_4:13214152-13214171 Msa1314300:three_prime_UTR 55.0%
ACCTTCCACACTCTTTCCGC+CGG + chr8_4:13215131-13215150 None:intergenic 55.0%
CAGGTCATCTGCAGGAAGTC+AGG - chr8_4:13215156-13215175 Msa1314300:intron 55.0%
GGCCTACTAGCAGCTCCATA+TGG - chr8_4:13215515-13215534 Msa1314300:intron 55.0%
CAGTAGGAATGCCTCCCGTT+CGG - chr8_4:13216095-13216114 Msa1314300:intron 55.0%
! AAGAGAGCTAGAGGCTCTGG+TGG - chr8_4:13216365-13216384 Msa1314300:intron 55.0%
ACTCGGATTCAGCCGAGTAG+TGG + chr8_4:13216441-13216460 None:intergenic 55.0%
ACTACTCGGCTGAATCCGAG+TGG - chr8_4:13216440-13216459 Msa1314300:intron 55.0%
GACGTCGGAACAAGTGGTTG+TGG + chr8_4:13216519-13216538 None:intergenic 55.0%
CAGGATTTGTCAGCCGATGC+CGG - chr8_4:13216617-13216636 Msa1314300:intron 55.0%
CGAAACCGGCGGAAAGAGTG+TGG - chr8_4:13215123-13215142 Msa1314300:intron 60.0%
! ACCGGCGGAAAGAGTGTGGA+AGG - chr8_4:13215127-13215146 Msa1314300:intron 60.0%
!! CGAACGGGAGGCATTCCTAC+TGG + chr8_4:13216097-13216116 None:intergenic 60.0%
GGCAAAGGAATCCGAACGGG+AGG + chr8_4:13216109-13216128 None:intergenic 60.0%
CGGATTCAGCCGAGTAGTGG+TGG + chr8_4:13216438-13216457 None:intergenic 60.0%
GATTTGTCAGCCGATGCCGG+TGG - chr8_4:13216620-13216639 Msa1314300:intron 60.0%
ATTCCGACCACCACCACCAC+CGG + chr8_4:13216639-13216658 None:intergenic 60.0%
! CATCTGGAGCGTGACCCGAG+TGG + chr8_4:13214674-13214693 None:intergenic 65.0%
TTGTCAGCCGATGCCGGTGG+TGG - chr8_4:13216623-13216642 Msa1314300:intron 65.0%
ACCACCACCACCACCGGCAT+CGG + chr8_4:13216633-13216652 None:intergenic 65.0%
!! ATGCCGGTGGTGGTGGTGGT+CGG - chr8_4:13216633-13216652 Msa1314300:intron 65.0%
! TCAGCCGATGCCGGTGGTGG+TGG - chr8_4:13216626-13216645 Msa1314300:intron 70.0%
!! GCCGATGCCGGTGGTGGTGG+TGG - chr8_4:13216629-13216648 Msa1314300:intron 75.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 13214151 13216966 13214151 ID=Msa1314300;Name=Msa1314300
chr8_4 mRNA 13214151 13216966 13214151 ID=Msa1314300-mRNA-1;Parent=Msa1314300;Name=Msa1314300-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.75|0.6|1|1|1|5|281|318
chr8_4 exon 13214151 13214788 13214151 ID=Msa1314300-mRNA-1:exon:4950;Parent=Msa1314300-mRNA-1
chr8_4 exon 13214871 13215038 13214871 ID=Msa1314300-mRNA-1:exon:4949;Parent=Msa1314300-mRNA-1
chr8_4 exon 13215567 13215641 13215567 ID=Msa1314300-mRNA-1:exon:4948;Parent=Msa1314300-mRNA-1
chr8_4 exon 13215895 13216043 13215895 ID=Msa1314300-mRNA-1:exon:4947;Parent=Msa1314300-mRNA-1
chr8_4 exon 13216759 13216966 13216759 ID=Msa1314300-mRNA-1:exon:4946;Parent=Msa1314300-mRNA-1
chr8_4 CDS 13216759 13216966 13216759 ID=Msa1314300-mRNA-1:cds;Parent=Msa1314300-mRNA-1
chr8_4 CDS 13215895 13216043 13215895 ID=Msa1314300-mRNA-1:cds;Parent=Msa1314300-mRNA-1
chr8_4 CDS 13215567 13215641 13215567 ID=Msa1314300-mRNA-1:cds;Parent=Msa1314300-mRNA-1
chr8_4 CDS 13214871 13215038 13214871 ID=Msa1314300-mRNA-1:cds;Parent=Msa1314300-mRNA-1
chr8_4 CDS 13214432 13214788 13214432 ID=Msa1314300-mRNA-1:cds;Parent=Msa1314300-mRNA-1
chr8_4 three_prime_UTR 13214151 13214431 13214151 ID=Msa1314300-mRNA-1:three_prime_utr;Parent=Msa1314300-mRNA-1
Gene Sequence

>Msa1314300

ATGAAGCACTCATGGCCATGGGGGACTTGTTCTGAATCTGGTGTGCAACAATCCTCTATGTCAGAAAATCTAACTTTGAATATGAGTGTTTTGCAACAAGAATGCAACAAGTCTAAACCACTAGGTTTTCAATTTCAAGACCAAGATTCTTCTTCTACTCAATCAACTGGTGGTCAATCTTATCTGGAAGTTGGTTCGGAACAATCCGGTCCAATTTCTGTCCAGTATAACAATTCCTCTGCTTGTTCAACACTTAACGAAACCGGCGGAAAGAGTGTGGAAGGTATCATCAGGTCATCTGCAGGAAGTCAGGATTTTACTTTGCCTCCTTCTCAACTGGATCACAACCAATCACTTGCTCATGTTGCATTCCCTCATGCTGAGACATACTCTAATGGCCTACTAGCAGCTCCATATGGATCACGGAATAATGTTAATCATGCTGAGCCAGTAGGAATGCCTCCCGTTCGGATTCCTTTGCCTCTTAATCTTTGTGAAGAACCAATATACGTGAATGCAAAGCAGTACCATGCTATTATGAGACGTAGACAGTATCGTGCAAAGCTCGAAGCACAGAACAAACTCGTCAAAAATCGGAAACCATATCTACATGAGTCGCGCCATCTGCATGCATTGAAGAGAGCTAGAGGCTCTGGTGGACGTTTTCTAAATACCAAAAAGCTCCAAGACCATGGCTTTAATGTTTCCACCACTACTCGGCTGAATCCGAGTGGAAATGTGCTGGAATCAAGGGTACATCAAGTAGAAAAGTATAGAGATGGTGCTTCCACAACCACTTGTTCCGACGTCACAAGTGCTTCTAATAGTGACGATATGTTCCAACAACATGAATCCGACTTCAGATCAAGTGGTTACCCTTCTCATATGCAGGATTTGTCAGCCGATGCCGGTGGTGGTGGTGGTCGGAATCAACACCGCCTATCTGTCCTTATGTGA
Protein sequence

>Msa1314300

MKHSWPWGTCSESGVQQSSMSENLTLNMSVLQQECNKSKPLGFQFQDQDSSSTQSTGGQSYLEVGSEQSGPISVQYNNSSACSTLNETGGKSVEGIIRSSAGSQDFTLPPSQLDHNQSLAHVAFPHAETYSNGLLAAPYGSRNNVNHAEPVGMPPVRIPLPLNLCEEPIYVNAKQYHAIMRRRQYRAKLEAQNKLVKNRKPYLHESRHLHALKRARGSGGRFLNTKKLQDHGFNVSTTTRLNPSGNVLESRVHQVEKYRDGASTTTCSDVTSASNSDDMFQQHESDFRSSGYPSHMQDLSADAGGGGGRNQHRLSVLM*