AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1233930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233930 MtrunA17_Chr8g0343651 85.448 268 34 3 1 266 1 265 2.35e-163 455
Msa1233930 MtrunA17_Chr1g0212191 51.045 335 94 4 2 266 3 337 5.26e-112 328
Msa1233930 MtrunA17_Chr1g0146851 31.048 248 153 5 18 257 143 380 5.28e-27 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1233930 AT5G08580.1 58.296 223 91 1 37 257 127 349 1.86e-92 279
Msa1233930 AT5G08580.2 58.296 223 91 1 37 257 127 349 1.86e-92 279
Msa1233930 AT4G27790.2 33.333 210 133 2 40 247 90 294 8.72e-34 125
Msa1233930 AT4G27790.1 33.333 210 133 2 40 247 90 294 4.10e-33 124

Find 77 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTCAATTGCAGGAAATTT+TGG 0.122576 8_2:+11101967 Msa1233930:intron
ATTGAACTGTTGCCTATAAT+TGG 0.126732 8_2:+11101327 Msa1233930:CDS
GTGGGGTCCACATCGATTTC+AGG 0.253730 8_2:-11099878 None:intergenic
CTTCAATTGGTGTTGCTAAT+AGG 0.259324 8_2:+11102144 Msa1233930:three_prime_UTR
CACAACCATTGTTGAAGCTT+TGG 0.271772 8_2:-11100750 None:intergenic
TTTCTATGGGCTCTATGATT+TGG 0.299108 8_2:+11101093 Msa1233930:CDS
GGTCTTTCCACTTCCACTTC+AGG 0.302288 8_2:+11100037 Msa1233930:CDS
AGCAACTAAATTATCGAATT+CGG 0.319533 8_2:+11099670 Msa1233930:five_prime_UTR
TCAACAGATGATGATCCTTT+TGG 0.329012 8_2:+11100514 Msa1233930:intron
GGATGGACTTTCCCAATTAT+AGG 0.336347 8_2:-11101339 None:intergenic
TCTTTATAGGGAAAGAGATA+TGG 0.353737 8_2:+11101039 Msa1233930:intron
CTCCCTCTCTCTGTCTCTGT+TGG 0.383026 8_2:-11099696 None:intergenic
TTTGTTTCCTTCTCTGAGTT+CGG 0.391698 8_2:+11100016 Msa1233930:CDS
TCATCGTAGCAGCAGGGAGC+TGG 0.401414 8_2:+11102079 Msa1233930:three_prime_UTR
CATCGGTAGTCATACAATAA+TGG 0.403446 8_2:+11102108 Msa1233930:three_prime_UTR
TTTAATGCATCAGATGTAGA+TGG 0.414146 8_2:+11100568 Msa1233930:CDS
ACAACCATTGTTGAAGCTTT+GGG 0.417958 8_2:-11100749 None:intergenic
TTGAACTGTTGCCTATAATT+GGG 0.419076 8_2:+11101328 Msa1233930:CDS
TTTGGGTTGTTGCTGTCAGC+CGG 0.421117 8_2:-11100732 None:intergenic
ACATCATTCTGATGATTCAA+TGG 0.432679 8_2:+11101150 Msa1233930:CDS
ATTCGTGAACGGAGACGAAC+TGG 0.437603 8_2:-11099904 None:intergenic
GAAAGAGATATGGACAGAGA+TGG 0.456266 8_2:+11101049 Msa1233930:CDS
TTGAGGATTGTTGTTGGTTG+AGG 0.463177 8_2:-11099792 None:intergenic
AAAGGGTTGAGGATTGTTGT+TGG 0.468893 8_2:-11099798 None:intergenic
CCACATCGATTTCAGGGAAT+AGG 0.469765 8_2:-11099871 None:intergenic
AAAGAGATATGGACAGAGAT+GGG 0.472960 8_2:+11101050 Msa1233930:CDS
ATTGGGAAAGTCCATCCATC+TGG 0.473215 8_2:+11101345 Msa1233930:CDS
ACAAAGTTCATCGTAGCAGC+AGG 0.474882 8_2:+11102072 Msa1233930:three_prime_UTR
ATGCAAAGAAATACATTACT+AGG 0.474990 8_2:-11102304 None:intergenic
AAGAGAACCTGAAGTGGAAG+TGG 0.479410 8_2:-11100044 None:intergenic
ATGAGTTCAATGTTACCAAC+AGG 0.482937 8_2:+11099840 Msa1233930:CDS
GGTTGTGCAAGGAGGAAGTC+TGG 0.485139 8_2:+11100766 Msa1233930:CDS
CATAATAATGTCCAGATGGA+TGG 0.490015 8_2:-11101356 None:intergenic
AGCTGTGCTAAACTCACGTT+GGG 0.496453 8_2:+11102208 Msa1233930:three_prime_UTR
ATCAGTATCAGTATGTTGAA+AGG 0.505257 8_2:-11099816 None:intergenic
TTCAATGGATGCTTCAGCTA+AGG 0.507239 8_2:+11101165 Msa1233930:CDS
TGGGGTCCACATCGATTTCA+GGG 0.507529 8_2:-11099877 None:intergenic
ACAGAGACAGAGAGAGGGAG+GGG 0.511316 8_2:+11099699 Msa1233930:five_prime_UTR
TTGGGTTGTTGCTGTCAGCC+GGG 0.514101 8_2:-11100731 None:intergenic
AGAAGAAAGAGAACCTGAAG+TGG 0.516527 8_2:-11100050 None:intergenic
TCAGTATCAGTATGTTGAAA+GGG 0.520104 8_2:-11099815 None:intergenic
TGTTGGTTGAGGAACGAGAG+AGG 0.526155 8_2:-11099781 None:intergenic
CATGAAATCAGTAAAGTAGT+TGG 0.526159 8_2:+11102178 Msa1233930:three_prime_UTR
CATCGTAGCAGCAGGGAGCT+GGG 0.526673 8_2:+11102080 Msa1233930:three_prime_UTR
AGCCTCATATCATAACCAAA+AGG 0.545851 8_2:-11100529 None:intergenic
CAACAGAGACAGAGAGAGGG+AGG 0.546422 8_2:+11099697 Msa1233930:five_prime_UTR
TGGTTATGATATGAGGCTGT+TGG 0.555595 8_2:+11100534 Msa1233930:CDS
TCAGTATGTTGAAAGGGTTG+AGG 0.572064 8_2:-11099809 None:intergenic
AGCAGAATACTTAATGTCAC+AGG 0.573374 8_2:+11101389 Msa1233930:CDS
ACGGAGACGAACTGGTCAGT+GGG 0.577199 8_2:-11099896 None:intergenic
GTTGGGCAATGATTATGAGC+AGG 0.584943 8_2:+11102225 Msa1233930:three_prime_UTR
TATGAGCAGGTGCAGCGAGT+AGG 0.586193 8_2:+11102238 Msa1233930:three_prime_UTR
CAGGGAGCTGGGACCAACAT+CGG 0.590979 8_2:+11102091 Msa1233930:three_prime_UTR
ATCCAACAGAGACAGAGAGA+GGG 0.593574 8_2:+11099694 Msa1233930:five_prime_UTR
ATCGATTTCAGGGAATAGGC+GGG 0.594459 8_2:-11099867 None:intergenic
TATTGTATGACTACCGATGT+TGG 0.601831 8_2:-11102104 None:intergenic
AACAGAGACAGAGAGAGGGA+GGG 0.603321 8_2:+11099698 Msa1233930:five_prime_UTR
ATTGGTGTTGCTAATAGGTG+TGG 0.605057 8_2:+11102149 Msa1233930:three_prime_UTR
GATTCTACATCGTAGTCAGA+AGG 0.611445 8_2:+11099961 Msa1233930:CDS
GCTTCAACAATGGTTGTGCA+AGG 0.612316 8_2:+11100755 Msa1233930:CDS
GTTGCATAATAATGTCCAGA+TGG 0.612930 8_2:-11101360 None:intergenic
GGAAAGACCGAACTCAGAGA+AGG 0.614168 8_2:-11100023 None:intergenic
GTTTATGATAAGAATCGCGA+TGG 0.617868 8_2:+11099992 Msa1233930:CDS
AACGGAGACGAACTGGTCAG+TGG 0.620182 8_2:-11099897 None:intergenic
CATCGATTTCAGGGAATAGG+CGG 0.620962 8_2:-11099868 None:intergenic
ACAACCCAAAGCTTCAACAA+TGG 0.624076 8_2:+11100745 Msa1233930:CDS
ACATCGTAGTCAGAAGGAGA+TGG 0.624127 8_2:+11099967 Msa1233930:CDS
TACGATGTAGAATCTGTCGT+TGG 0.627152 8_2:-11099952 None:intergenic
CCACTGAGTTAATTCGTGAA+CGG 0.629696 8_2:-11099915 None:intergenic
CCTATTCCCTGAAATCGATG+TGG 0.637700 8_2:+11099871 Msa1233930:CDS
CAGCTGTGCTAAACTCACGT+TGG 0.640745 8_2:+11102207 Msa1233930:three_prime_UTR
CAAAGTTCATCGTAGCAGCA+GGG 0.646105 8_2:+11102073 Msa1233930:three_prime_UTR
AATCCAACAGAGACAGAGAG+AGG 0.656000 8_2:+11099693 Msa1233930:five_prime_UTR
GCATCAGATGTAGATGGTGA+TGG 0.658089 8_2:+11100574 Msa1233930:CDS
CGGAGACGAACTGGTCAGTG+GGG 0.682692 8_2:-11099895 None:intergenic
TCAACAATGGTTGTGCAAGG+AGG 0.686528 8_2:+11100758 Msa1233930:CDS
CCGTTCACGAATTAACTCAG+TGG 0.718749 8_2:+11099915 Msa1233930:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCAAATACATAATATAATT+TGG - chr8_2:11100101-11100120 None:intergenic 10.0%
!!! TCATAATTTTTTTAATTTGA+AGG + chr8_2:11100199-11100218 Msa1233930:intron 10.0%
!!! AAAAATTAGTTTGAATAAAT+AGG - chr8_2:11100687-11100706 None:intergenic 10.0%
!!! TTTCTAAGTTTTCATTTTTT+TGG + chr8_2:11100279-11100298 Msa1233930:intron 15.0%
!! CTTAGAAAATAAAAATACTA+TGG - chr8_2:11100843-11100862 None:intergenic 15.0%
!! AGAGAATTGAAAAATTTAAA+AGG - chr8_2:11100872-11100891 None:intergenic 15.0%
!! ATCTTAAAAAAACATATCAA+TGG + chr8_2:11102325-11102344 Msa1233930:three_prime_UTR 15.0%
!! TCTTAAAAAAACATATCAAT+GGG + chr8_2:11102326-11102345 Msa1233930:three_prime_UTR 15.0%
!! ATTTGGAAACAAGAAAAAAA+AGG - chr8_2:11100084-11100103 None:intergenic 20.0%
!!! TTTAAGTTCTAAGTTGTTTA+CGG + chr8_2:11100136-11100155 Msa1233930:intron 20.0%
!! TGATAATTGAATACATCATA+TGG + chr8_2:11100404-11100423 Msa1233930:intron 20.0%
!!! TAAATTTTTCAATTCTCTGT+TGG + chr8_2:11100874-11100893 Msa1233930:intron 20.0%
!!! AAATTTTTCAATTCTCTGTT+GGG + chr8_2:11100875-11100894 Msa1233930:intron 20.0%
!! TTCCCTATAAAGAAATAAAA+GGG - chr8_2:11101032-11101051 None:intergenic 20.0%
!! TTTCCCTATAAAGAAATAAA+AGG - chr8_2:11101033-11101052 None:intergenic 20.0%
!!! AAGCATTGTAAAAACTAATT+TGG + chr8_2:11101584-11101603 Msa1233930:intron 20.0%
!!! ATTATTTGTTTCTATTTTCC+CGG + chr8_2:11101848-11101867 Msa1233930:intron 20.0%
!!! ATCAATGGGAATATTTTTTA+TGG + chr8_2:11102340-11102359 Msa1233930:three_prime_UTR 20.0%
! AGCAACTAAATTATCGAATT+CGG + chr8_2:11099670-11099689 Msa1233930:five_prime_UTR 25.0%
!!! TTTTAGGGATTCTTAATTTG+TGG + chr8_2:11100168-11100187 Msa1233930:intron 25.0%
!!! TTTAGGGATTCTTAATTTGT+GGG + chr8_2:11100169-11100188 Msa1233930:intron 25.0%
! TTCATGTGACATAATCAATT+TGG + chr8_2:11100362-11100381 Msa1233930:intron 25.0%
! AATTCAATACACAGTCATAA+AGG - chr8_2:11100470-11100489 None:intergenic 25.0%
!!! TGCCCTTTTATTTCTTTATA+GGG + chr8_2:11101027-11101046 Msa1233930:intron 25.0%
!!! AGTTTTAGAGAGTTTTTCTA+TGG + chr8_2:11101079-11101098 Msa1233930:CDS 25.0%
!!! GTTTTAGAGAGTTTTTCTAT+GGG + chr8_2:11101080-11101099 Msa1233930:CDS 25.0%
! CTATATATTGACACAAAGAA+AGG - chr8_2:11101232-11101251 None:intergenic 25.0%
!!! ACAACATCCATTTTTTCTTA+TGG + chr8_2:11101268-11101287 Msa1233930:intron 25.0%
!!! CAACATCCATTTTTTCTTAT+GGG + chr8_2:11101269-11101288 Msa1233930:intron 25.0%
! TCAAAACCCATAAGAAAAAA+TGG - chr8_2:11101278-11101297 None:intergenic 25.0%
!!! TTTTGATTTCAATATGTTGC+AGG + chr8_2:11101292-11101311 Msa1233930:intron 25.0%
!!! AGTTCTTTTAAGTACATGTT+AGG + chr8_2:11101874-11101893 Msa1233930:intron 25.0%
!!! TTTTAAGTACATGTTAGGTA+AGG + chr8_2:11101879-11101898 Msa1233930:intron 25.0%
! TTTAAGTACATGTTAGGTAA+GGG + chr8_2:11101880-11101899 Msa1233930:intron 25.0%
!!! ATACAATAATGGACAAAAAC+TGG + chr8_2:11102119-11102138 Msa1233930:three_prime_UTR 25.0%
! ATGCAAAGAAATACATTACT+AGG - chr8_2:11102307-11102326 None:intergenic 25.0%
TCAGTATCAGTATGTTGAAA+GGG - chr8_2:11099818-11099837 None:intergenic 30.0%
ATCAGTATCAGTATGTTGAA+AGG - chr8_2:11099819-11099838 None:intergenic 30.0%
!! TTAGGGATTCTTAATTTGTG+GGG + chr8_2:11100170-11100189 Msa1233930:intron 30.0%
! TTTTGATCCTTGCAAGAATT+AGG + chr8_2:11100323-11100342 Msa1233930:intron 30.0%
TTTGATCCTTGCAAGAATTA+GGG + chr8_2:11100324-11100343 Msa1233930:intron 30.0%
!! ATCCTTTTGGTTATGATATG+AGG + chr8_2:11100527-11100546 Msa1233930:CDS 30.0%
TTTAATGCATCAGATGTAGA+TGG + chr8_2:11100568-11100587 Msa1233930:CDS 30.0%
!! GTGCCCTTTTATTTCTTTAT+AGG + chr8_2:11101026-11101045 Msa1233930:intron 30.0%
TCTTTATAGGGAAAGAGATA+TGG + chr8_2:11101039-11101058 Msa1233930:intron 30.0%
ACATCATTCTGATGATTCAA+TGG + chr8_2:11101150-11101169 Msa1233930:CDS 30.0%
ATTGAACTGTTGCCTATAAT+TGG + chr8_2:11101327-11101346 Msa1233930:CDS 30.0%
TTGAACTGTTGCCTATAATT+GGG + chr8_2:11101328-11101347 Msa1233930:CDS 30.0%
CAATCATCTCAGTCAAATTA+AGG - chr8_2:11101815-11101834 None:intergenic 30.0%
TTTCTGTAATCTTCAATTGC+AGG + chr8_2:11101958-11101977 Msa1233930:intron 30.0%
! TCTTCAATTGCAGGAAATTT+TGG + chr8_2:11101967-11101986 Msa1233930:intron 30.0%
TGCGAAAAAATTGCAATGAT+AGG - chr8_2:11102014-11102033 None:intergenic 30.0%
!!! TGCAATTTTTTCGCAATATC+AGG + chr8_2:11102019-11102038 Msa1233930:CDS 30.0%
ACAAAAACTGGATCTTCAAT+TGG + chr8_2:11102131-11102150 Msa1233930:three_prime_UTR 30.0%
CATGAAATCAGTAAAGTAGT+TGG + chr8_2:11102178-11102197 Msa1233930:three_prime_UTR 30.0%
ATGAGTTCAATGTTACCAAC+AGG + chr8_2:11099840-11099859 Msa1233930:CDS 35.0%
GTTTATGATAAGAATCGCGA+TGG + chr8_2:11099992-11100011 Msa1233930:CDS 35.0%
TTTGTTTCCTTCTCTGAGTT+CGG + chr8_2:11100016-11100035 Msa1233930:CDS 35.0%
! TCAACAGATGATGATCCTTT+TGG + chr8_2:11100514-11100533 Msa1233930:intron 35.0%
AGCCTCATATCATAACCAAA+AGG - chr8_2:11100532-11100551 None:intergenic 35.0%
TAGATACTGTGGTGAATGAA+CGG + chr8_2:11100657-11100676 Msa1233930:intron 35.0%
ACAACCATTGTTGAAGCTTT+GGG - chr8_2:11100752-11100771 None:intergenic 35.0%
CCAATATTGTGACTTCTTCT+AGG - chr8_2:11100986-11101005 None:intergenic 35.0%
CCTAGAAGAAGTCACAATAT+TGG + chr8_2:11100983-11101002 Msa1233930:intron 35.0%
!! GGAATACCTTTTTAAGAGAC+TGG + chr8_2:11101004-11101023 Msa1233930:intron 35.0%
AAAGAGATATGGACAGAGAT+GGG + chr8_2:11101050-11101069 Msa1233930:CDS 35.0%
TTTCTATGGGCTCTATGATT+TGG + chr8_2:11101093-11101112 Msa1233930:CDS 35.0%
! CAGCTTGACAAAGATTTTGA+CGG + chr8_2:11101199-11101218 Msa1233930:CDS 35.0%
!! AGCTTGACAAAGATTTTGAC+GGG + chr8_2:11101200-11101219 Msa1233930:CDS 35.0%
AAATGGATGTTGTGCTATAG+AGG - chr8_2:11101261-11101280 None:intergenic 35.0%
CATAATAATGTCCAGATGGA+TGG - chr8_2:11101359-11101378 None:intergenic 35.0%
GTTGCATAATAATGTCCAGA+TGG - chr8_2:11101363-11101382 None:intergenic 35.0%
AGCAGAATACTTAATGTCAC+AGG + chr8_2:11101389-11101408 Msa1233930:CDS 35.0%
CAACAGCTACAGTGTAATTA+TGG - chr8_2:11101432-11101451 None:intergenic 35.0%
! GTGTCATTCCTTTCTTACAA+AGG + chr8_2:11101458-11101477 Msa1233930:intron 35.0%
ACAACGATCATTCACAAGTT+CGG - chr8_2:11101487-11101506 None:intergenic 35.0%
! GTTCATAGCCATCATACTTT+TGG - chr8_2:11101641-11101660 None:intergenic 35.0%
AGCAAGCAACTATGTGAATA+GGG + chr8_2:11101666-11101685 Msa1233930:intron 35.0%
!!! CTATCTTACTCGCTACTTTT+TGG - chr8_2:11101696-11101715 None:intergenic 35.0%
TTAACAAGCACTACAACATC+AGG + chr8_2:11101717-11101736 Msa1233930:intron 35.0%
GTTATCATAGCAGCATAGAA+AGG - chr8_2:11101767-11101786 None:intergenic 35.0%
TTCTATGCTGCTATGATAAC+AGG + chr8_2:11101767-11101786 Msa1233930:intron 35.0%
AGGCACAAATTGACAATGAT+GGG + chr8_2:11101787-11101806 Msa1233930:intron 35.0%
TGTACTTAAAAGAACTCGTC+CGG - chr8_2:11101870-11101889 None:intergenic 35.0%
!!! TGAGTCAGTTTTGAGTTGAA+TGG + chr8_2:11102048-11102067 Msa1233930:exon 35.0%
TATTGTATGACTACCGATGT+TGG - chr8_2:11102107-11102126 None:intergenic 35.0%
CATCGGTAGTCATACAATAA+TGG + chr8_2:11102108-11102127 Msa1233930:three_prime_UTR 35.0%
!! CTTCAATTGGTGTTGCTAAT+AGG + chr8_2:11102144-11102163 Msa1233930:three_prime_UTR 35.0%
! TTGAGGATTGTTGTTGGTTG+AGG - chr8_2:11099795-11099814 None:intergenic 40.0%
AAAGGGTTGAGGATTGTTGT+TGG - chr8_2:11099801-11099820 None:intergenic 40.0%
TCAGTATGTTGAAAGGGTTG+AGG - chr8_2:11099812-11099831 None:intergenic 40.0%
CCACTGAGTTAATTCGTGAA+CGG - chr8_2:11099918-11099937 None:intergenic 40.0%
TACGATGTAGAATCTGTCGT+TGG - chr8_2:11099955-11099974 None:intergenic 40.0%
GATTCTACATCGTAGTCAGA+AGG + chr8_2:11099961-11099980 Msa1233930:CDS 40.0%
AGAAGAAAGAGAACCTGAAG+TGG - chr8_2:11100053-11100072 None:intergenic 40.0%
TAAGTTGTTTACGGCAAGCA+AGG + chr8_2:11100145-11100164 Msa1233930:intron 40.0%
! TTTACGGCAAGCAAGGTTTT+AGG + chr8_2:11100152-11100171 Msa1233930:intron 40.0%
! TTACGGCAAGCAAGGTTTTA+GGG + chr8_2:11100153-11100172 Msa1233930:intron 40.0%
TGGTTATGATATGAGGCTGT+TGG + chr8_2:11100534-11100553 Msa1233930:CDS 40.0%
TCATGCACAACTAGATACTG+TGG + chr8_2:11100646-11100665 Msa1233930:intron 40.0%
! GTCTTGTAGTTTTCTTCACC+CGG + chr8_2:11100713-11100732 Msa1233930:intron 40.0%
ACAACCCAAAGCTTCAACAA+TGG + chr8_2:11100745-11100764 Msa1233930:CDS 40.0%
CACAACCATTGTTGAAGCTT+TGG - chr8_2:11100753-11100772 None:intergenic 40.0%
! ATTGTGACTTCTTCTAGGAG+AGG - chr8_2:11100981-11101000 None:intergenic 40.0%
GAAAGAGATATGGACAGAGA+TGG + chr8_2:11101049-11101068 Msa1233930:CDS 40.0%
TTCAATGGATGCTTCAGCTA+AGG + chr8_2:11101165-11101184 Msa1233930:CDS 40.0%
! GGATGGACTTTCCCAATTAT+AGG - chr8_2:11101342-11101361 None:intergenic 40.0%
TACACTGTAGCTGTTGACAA+AGG + chr8_2:11101436-11101455 Msa1233930:intron 40.0%
TTCGGATGCCTTTGTAAGAA+AGG - chr8_2:11101469-11101488 None:intergenic 40.0%
GCGTAGAAATTGACCAAGAT+GGG + chr8_2:11101606-11101625 Msa1233930:intron 40.0%
! CAACAACGCCAAAAGTATGA+TGG + chr8_2:11101630-11101649 Msa1233930:intron 40.0%
GAGCAAGCAACTATGTGAAT+AGG + chr8_2:11101665-11101684 Msa1233930:intron 40.0%
CAGGCACAAATTGACAATGA+TGG + chr8_2:11101786-11101805 Msa1233930:intron 40.0%
GTACTTAAAAGAACTCGTCC+GGG - chr8_2:11101869-11101888 None:intergenic 40.0%
!! ATTGGTGTTGCTAATAGGTG+TGG + chr8_2:11102149-11102168 Msa1233930:three_prime_UTR 40.0%
AATCCAACAGAGACAGAGAG+AGG + chr8_2:11099693-11099712 Msa1233930:five_prime_UTR 45.0%
ATCCAACAGAGACAGAGAGA+GGG + chr8_2:11099694-11099713 Msa1233930:five_prime_UTR 45.0%
! ATCGATTTCAGGGAATAGGC+GGG - chr8_2:11099870-11099889 None:intergenic 45.0%
!! CATCGATTTCAGGGAATAGG+CGG - chr8_2:11099871-11099890 None:intergenic 45.0%
!! CCACATCGATTTCAGGGAAT+AGG - chr8_2:11099874-11099893 None:intergenic 45.0%
CCTATTCCCTGAAATCGATG+TGG + chr8_2:11099871-11099890 Msa1233930:CDS 45.0%
CCGTTCACGAATTAACTCAG+TGG + chr8_2:11099915-11099934 Msa1233930:CDS 45.0%
ACATCGTAGTCAGAAGGAGA+TGG + chr8_2:11099967-11099986 Msa1233930:CDS 45.0%
AAGAGAACCTGAAGTGGAAG+TGG - chr8_2:11100047-11100066 None:intergenic 45.0%
CTCACTCCCTAATTCTTGCA+AGG - chr8_2:11100333-11100352 None:intergenic 45.0%
GCATCAGATGTAGATGGTGA+TGG + chr8_2:11100574-11100593 Msa1233930:CDS 45.0%
GCTTCAACAATGGTTGTGCA+AGG + chr8_2:11100755-11100774 Msa1233930:CDS 45.0%
TCAACAATGGTTGTGCAAGG+AGG + chr8_2:11100758-11100777 Msa1233930:CDS 45.0%
! AGGGCACCAGTCTCTTAAAA+AGG - chr8_2:11101013-11101032 None:intergenic 45.0%
ATTGGGAAAGTCCATCCATC+TGG + chr8_2:11101345-11101364 Msa1233930:CDS 45.0%
GGCGTAGAAATTGACCAAGA+TGG + chr8_2:11101605-11101624 Msa1233930:intron 45.0%
ACAAGCACTACAACATCAGG+TGG + chr8_2:11101720-11101739 Msa1233930:intron 45.0%
ACAAAGTTCATCGTAGCAGC+AGG + chr8_2:11102072-11102091 Msa1233930:three_prime_UTR 45.0%
CAAAGTTCATCGTAGCAGCA+GGG + chr8_2:11102073-11102092 Msa1233930:three_prime_UTR 45.0%
AGCTGTGCTAAACTCACGTT+GGG + chr8_2:11102208-11102227 Msa1233930:three_prime_UTR 45.0%
GTTGGGCAATGATTATGAGC+AGG + chr8_2:11102225-11102244 Msa1233930:three_prime_UTR 45.0%
!!! ATCAATTTTTAAATAAAATA+AGG - chr8_2:11100388-11100407 None:intergenic 5.0%
AACAGAGACAGAGAGAGGGA+GGG + chr8_2:11099698-11099717 Msa1233930:five_prime_UTR 50.0%
! TGTTGGTTGAGGAACGAGAG+AGG - chr8_2:11099784-11099803 None:intergenic 50.0%
!! TGGGGTCCACATCGATTTCA+GGG - chr8_2:11099880-11099899 None:intergenic 50.0%
ATTCGTGAACGGAGACGAAC+TGG - chr8_2:11099907-11099926 None:intergenic 50.0%
GGAAAGACCGAACTCAGAGA+AGG - chr8_2:11100026-11100045 None:intergenic 50.0%
GGTCTTTCCACTTCCACTTC+AGG + chr8_2:11100037-11100056 Msa1233930:CDS 50.0%
!! TTTGGGTTGTTGCTGTCAGC+CGG - chr8_2:11100735-11100754 None:intergenic 50.0%
TTGGCGTTGTTGACCCATCT+TGG - chr8_2:11101622-11101641 None:intergenic 50.0%
CAGCTGTGCTAAACTCACGT+TGG + chr8_2:11102207-11102226 Msa1233930:three_prime_UTR 50.0%
CTCCCTCTCTCTGTCTCTGT+TGG - chr8_2:11099699-11099718 None:intergenic 55.0%
CAACAGAGACAGAGAGAGGG+AGG + chr8_2:11099697-11099716 Msa1233930:five_prime_UTR 55.0%
ACAGAGACAGAGAGAGGGAG+GGG + chr8_2:11099699-11099718 Msa1233930:five_prime_UTR 55.0%
!! GTGGGGTCCACATCGATTTC+AGG - chr8_2:11099881-11099900 None:intergenic 55.0%
ACGGAGACGAACTGGTCAGT+GGG - chr8_2:11099899-11099918 None:intergenic 55.0%
AACGGAGACGAACTGGTCAG+TGG - chr8_2:11099900-11099919 None:intergenic 55.0%
! TTGGGTTGTTGCTGTCAGCC+GGG - chr8_2:11100734-11100753 None:intergenic 55.0%
GGTTGTGCAAGGAGGAAGTC+TGG + chr8_2:11100766-11100785 Msa1233930:CDS 55.0%
ATCCCCGCTAATCGCTTGTG+AGG + chr8_2:11100900-11100919 Msa1233930:intron 55.0%
TCCCCGCTAATCGCTTGTGA+GGG + chr8_2:11100901-11100920 Msa1233930:intron 55.0%
TCCCTCACAAGCGATTAGCG+GGG - chr8_2:11100905-11100924 None:intergenic 55.0%
GTCCCTCACAAGCGATTAGC+GGG - chr8_2:11100906-11100925 None:intergenic 55.0%
CGTCCCTCACAAGCGATTAG+CGG - chr8_2:11100907-11100926 None:intergenic 55.0%
TATGAGCAGGTGCAGCGAGT+AGG + chr8_2:11102238-11102257 Msa1233930:three_prime_UTR 55.0%
GAATAGGCGGGCGATCCTGT+TGG - chr8_2:11099858-11099877 None:intergenic 60.0%
CGGAGACGAACTGGTCAGTG+GGG - chr8_2:11099898-11099917 None:intergenic 60.0%
TCATCGTAGCAGCAGGGAGC+TGG + chr8_2:11102079-11102098 Msa1233930:three_prime_UTR 60.0%
CATCGTAGCAGCAGGGAGCT+GGG + chr8_2:11102080-11102099 Msa1233930:three_prime_UTR 60.0%
CAGGGAGCTGGGACCAACAT+CGG + chr8_2:11102091-11102110 Msa1233930:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 11099656 11102409 11099656 ID=Msa1233930;Name=Msa1233930
chr8_2 mRNA 11099656 11102409 11099656 ID=Msa1233930-mRNA-1;Parent=Msa1233930;Name=Msa1233930-mRNA-1;_AED=0.15;_eAED=0.15;_QI=72|0.8|1|1|1|1|6|343|285
chr8_2 exon 11099656 11100058 11099656 ID=Msa1233930-mRNA-1:exon:4320;Parent=Msa1233930-mRNA-1
chr8_2 exon 11100521 11100623 11100521 ID=Msa1233930-mRNA-1:exon:4321;Parent=Msa1233930-mRNA-1
chr8_2 exon 11100722 11100787 11100722 ID=Msa1233930-mRNA-1:exon:4322;Parent=Msa1233930-mRNA-1
chr8_2 exon 11101048 11101221 11101048 ID=Msa1233930-mRNA-1:exon:4323;Parent=Msa1233930-mRNA-1
chr8_2 exon 11101314 11101410 11101314 ID=Msa1233930-mRNA-1:exon:4324;Parent=Msa1233930-mRNA-1
chr8_2 exon 11101980 11102409 11101980 ID=Msa1233930-mRNA-1:exon:4325;Parent=Msa1233930-mRNA-1
chr8_2 five_prime_UTR 11099656 11099727 11099656 ID=Msa1233930-mRNA-1:five_prime_utr;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11099728 11100058 11099728 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11100521 11100623 11100521 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11100722 11100787 11100722 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11101048 11101221 11101048 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11101314 11101410 11101314 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 CDS 11101980 11102066 11101980 ID=Msa1233930-mRNA-1:cds;Parent=Msa1233930-mRNA-1
chr8_2 three_prime_UTR 11102067 11102409 11102067 ID=Msa1233930-mRNA-1:three_prime_utr;Parent=Msa1233930-mRNA-1
Gene Sequence

>Msa1233930

ATGATGAAATCGATATTTGTTTACACAATAATCACACTTGTTGTGCTCTTTCTCCTCTCTCGTTCCTCAACCAACAACAATCCTCAACCCTTTCAACATACTGATACTGATGATGAGTTCAATGTTACCAACAGGATCGCCCGCCTATTCCCTGAAATCGATGTGGACCCCACTGACCAGTTCGTCTCCGTTCACGAATTAACTCAGTGGAACGTTCATCATGTCCAACGACAGATTCTACATCGTAGTCAGAAGGAGATGGTCGTTTATGATAAGAATCGCGATGGTTTTGTTTCCTTCTCTGAGTTCGGTCTTTCCACTTCCACTTCAGATGATGATCCTTTTGGTTATGATATGAGGCTGTTGGAAGAAGAACATTTTAATGCATCAGATGTAGATGGTGATGGTCTTCTGAACTTATCTGAATTCAATGATTTTCTTCACCCGGCTGACAGCAACAACCCAAAGCTTCAACAATGGTTGTGCAAGGAGGAAGTCTGGGAAAGAGATATGGACAGAGATGGGAAAGTCAGTTTTAGAGAGTTTTTCTATGGGCTCTATGATTTGGTAAGCAACTATGATGAAAAAAGTCACAATGATTCACATCATTCTGATGATTCAATGGATGCTTCAGCTAAGGTTTTGTTTTCTCAGCTTGACAAAGATTTTGACGGGTATTTGTCAGATATTGAACTGTTGCCTATAATTGGGAAAGTCCATCCATCTGGACATTATTATGCAACAAAACAAGCAGAATACTTAATGTCACAGGAAATTTTGGCAAATGAGTCTAGATTTGAATCCTATCATTGCAATTTTTTCGCAATATCAGGTGACAATGAGTCAGTTTTGAGTTGA
Protein sequence

>Msa1233930

MMKSIFVYTIITLVVLFLLSRSSTNNNPQPFQHTDTDDEFNVTNRIARLFPEIDVDPTDQFVSVHELTQWNVHHVQRQILHRSQKEMVVYDKNRDGFVSFSEFGLSTSTSDDDPFGYDMRLLEEEHFNASDVDGDGLLNLSEFNDFLHPADSNNPKLQQWLCKEEVWERDMDRDGKVSFREFFYGLYDLVSNYDEKSHNDSHHSDDSMDASAKVLFSQLDKDFDGYLSDIELLPIIGKVHPSGHYYATKQAEYLMSQEILANESRFESYHCNFFAISGDNESVLS*