Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1234570 | XP_003627435.1 | 87.629 | 97 | 11 | 1 | 1 | 96 | 1 | 97 | 5.95e-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1234570 | Msa1315420 | 0.917098 | 8.025541e-86 | -8.615850e-47 |
| Msa1175040 | Msa1234570 | 0.809264 | 2.061730e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1234570 | MtrunA17_Chr8g0345021 | 87.629 | 97 | 11 | 1 | 1 | 96 | 1 | 97 | 5.47e-59 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTTCCTTATGAATAAA+AGG | 0.171857 | 8_2:-11910263 | Msa1234570:three_prime_UTR |
| GGAATCCAGTTTCCAGTCTT+AGG | 0.287752 | 8_2:+11910455 | None:intergenic |
| TAGACACAAACTTATTTCTA+AGG | 0.311347 | 8_2:+11910399 | None:intergenic |
| CCAAATCTCTTCTTCATATC+AGG | 0.317489 | 8_2:-11910914 | Msa1234570:intron |
| ACCTCTCCAAAGTGATTCTC+TGG | 0.376974 | 8_2:+11910434 | None:intergenic |
| CCTGATATGAAGAAGAGATT+TGG | 0.380350 | 8_2:+11910914 | None:intergenic |
| GAGATCCTAAGACTGGAAAC+TGG | 0.415942 | 8_2:-11910460 | Msa1234570:CDS |
| TAACATGAATGAATCAATAT+CGG | 0.448963 | 8_2:-11910516 | Msa1234570:CDS |
| ATAAGGAAATTCTTAAGTAA+AGG | 0.469042 | 8_2:+11910273 | None:intergenic |
| GAATTTCCTTATGAATAAAA+GGG | 0.479917 | 8_2:-11910262 | Msa1234570:three_prime_UTR |
| TGGATTCCAGAGAATCACTT+TGG | 0.493363 | 8_2:-11910440 | Msa1234570:CDS |
| AGATTTGGCAGCATTAATGT+TGG | 0.503666 | 8_2:+11910929 | None:intergenic |
| TGGATGAGAGATCCTAAGAC+TGG | 0.526718 | 8_2:-11910467 | Msa1234570:CDS |
| AGTCTTCACCACCTGCATGT+TGG | 0.574329 | 8_2:+11910538 | None:intergenic |
| TCCAGAGAATCACTTTGGAG+AGG | 0.599206 | 8_2:-11910435 | Msa1234570:CDS |
| GGCAGCATTAATGTTGGACA+TGG | 0.637036 | 8_2:+11910935 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAACAAATTATCAAA+AGG | + | chr8_2:11910437-11910456 | None:intergenic | 10.0% |
| !!! | ATATTTTCCTTTAATTTTTT+TGG | - | chr8_2:11910538-11910557 | Msa1234570:CDS | 10.0% |
| !! | AATATTTAATACAAAATAGA+AGG | + | chr8_2:11910593-11910612 | None:intergenic | 10.0% |
| !! | AAATTTAACAAATTATCAAA+AGG | + | chr8_2:11910437-11910456 | None:intergenic | 10.0% |
| !!! | ATATTTTCCTTTAATTTTTT+TGG | - | chr8_2:11910538-11910557 | Msa1234570:CDS | 10.0% |
| !! | AATATTTAATACAAAATAGA+AGG | + | chr8_2:11910593-11910612 | None:intergenic | 10.0% |
| !!! | ATTTATCGTTGTTTTTTTAA+AGG | + | chr8_2:11910374-11910393 | None:intergenic | 15.0% |
| !! | ATTTGTTTCAAAATTGAATT+TGG | + | chr8_2:11910520-11910539 | None:intergenic | 15.0% |
| !!! | ATTTATCGTTGTTTTTTTAA+AGG | + | chr8_2:11910374-11910393 | None:intergenic | 15.0% |
| !! | ATTTGTTTCAAAATTGAATT+TGG | + | chr8_2:11910520-11910539 | None:intergenic | 15.0% |
| !!! | TTGTTTTGTACTTTTTACAA+TGG | - | chr8_2:11910253-11910272 | Msa1234570:three_prime_UTR | 20.0% |
| !!! | TTTTTATTCTCTAGAAAGAA+AGG | - | chr8_2:11910454-11910473 | Msa1234570:CDS | 20.0% |
| !! | ATGCAAACCAAAAAAATTAA+AGG | + | chr8_2:11910548-11910567 | None:intergenic | 20.0% |
| !! | TAACATGAATGAATCAATAT+CGG | - | chr8_2:11910704-11910723 | Msa1234570:intron | 20.0% |
| !!! | AAATATTTTTCTCTCTTCAT+TGG | + | chr8_2:11910733-11910752 | None:intergenic | 20.0% |
| !!! | ATGAAGAGAGAAAAATATTT+TGG | - | chr8_2:11910733-11910752 | Msa1234570:intron | 20.0% |
| !! | ATAAGGAAATTCTTAAGTAA+AGG | + | chr8_2:11910950-11910969 | None:intergenic | 20.0% |
| !! | AGAATTTCCTTATGAATAAA+AGG | - | chr8_2:11910957-11910976 | Msa1234570:exon | 20.0% |
| !! | GAATTTCCTTATGAATAAAA+GGG | - | chr8_2:11910958-11910977 | Msa1234570:exon | 20.0% |
| !!! | TTGTTTTGTACTTTTTACAA+TGG | - | chr8_2:11910253-11910272 | Msa1234570:three_prime_UTR | 20.0% |
| !!! | TTTTTATTCTCTAGAAAGAA+AGG | - | chr8_2:11910454-11910473 | Msa1234570:CDS | 20.0% |
| !! | ATGCAAACCAAAAAAATTAA+AGG | + | chr8_2:11910548-11910567 | None:intergenic | 20.0% |
| !! | TAACATGAATGAATCAATAT+CGG | - | chr8_2:11910704-11910723 | Msa1234570:intron | 20.0% |
| !!! | AAATATTTTTCTCTCTTCAT+TGG | + | chr8_2:11910733-11910752 | None:intergenic | 20.0% |
| !!! | ATGAAGAGAGAAAAATATTT+TGG | - | chr8_2:11910733-11910752 | Msa1234570:intron | 20.0% |
| !! | ATAAGGAAATTCTTAAGTAA+AGG | + | chr8_2:11910950-11910969 | None:intergenic | 20.0% |
| !! | AGAATTTCCTTATGAATAAA+AGG | - | chr8_2:11910957-11910976 | Msa1234570:exon | 20.0% |
| !! | GAATTTCCTTATGAATAAAA+GGG | - | chr8_2:11910958-11910977 | Msa1234570:exon | 20.0% |
| ! | AAAGTACAAAACAAAAAGTG+AGG | + | chr8_2:11910247-11910266 | None:intergenic | 25.0% |
| !!! | TAGACACAAACTTATTTCTA+AGG | + | chr8_2:11910824-11910843 | None:intergenic | 25.0% |
| !! | TTTGATCCCTTTTATTCATA+AGG | + | chr8_2:11910967-11910986 | None:intergenic | 25.0% |
| ! | AAAGTACAAAACAAAAAGTG+AGG | + | chr8_2:11910247-11910266 | None:intergenic | 25.0% |
| !!! | TAGACACAAACTTATTTCTA+AGG | + | chr8_2:11910824-11910843 | None:intergenic | 25.0% |
| !! | TTTGATCCCTTTTATTCATA+AGG | + | chr8_2:11910967-11910986 | None:intergenic | 25.0% |
| !!! | CAAAAAGTGAGGTTTTTTAG+AGG | + | chr8_2:11910236-11910255 | None:intergenic | 30.0% |
| AGGAAAAAAACACATGAAGA+AGG | + | chr8_2:11910573-11910592 | None:intergenic | 30.0% | |
| !!! | CAAAAAGTGAGGTTTTTTAG+AGG | + | chr8_2:11910236-11910255 | None:intergenic | 30.0% |
| AGGAAAAAAACACATGAAGA+AGG | + | chr8_2:11910573-11910592 | None:intergenic | 30.0% | |
| AGATTTGGCAGCATTAATGT+TGG | + | chr8_2:11910294-11910313 | None:intergenic | 35.0% | |
| CCTGATATGAAGAAGAGATT+TGG | + | chr8_2:11910309-11910328 | None:intergenic | 35.0% | |
| CCAAATCTCTTCTTCATATC+AGG | - | chr8_2:11910306-11910325 | Msa1234570:three_prime_UTR | 35.0% | |
| CAAAAAGTTCAAGGTTGCAA+GGG | - | chr8_2:11910652-11910671 | Msa1234570:intron | 35.0% | |
| AGATTTGGCAGCATTAATGT+TGG | + | chr8_2:11910294-11910313 | None:intergenic | 35.0% | |
| CCTGATATGAAGAAGAGATT+TGG | + | chr8_2:11910309-11910328 | None:intergenic | 35.0% | |
| CCAAATCTCTTCTTCATATC+AGG | - | chr8_2:11910306-11910325 | Msa1234570:three_prime_UTR | 35.0% | |
| CAAAAAGTTCAAGGTTGCAA+GGG | - | chr8_2:11910652-11910671 | Msa1234570:intron | 35.0% | |
| TCTATTGATGTGTAGAGAGC+TGG | + | chr8_2:11910347-11910366 | None:intergenic | 40.0% | |
| CAGTTGCAGCAAAAAGTTCA+AGG | - | chr8_2:11910643-11910662 | Msa1234570:intron | 40.0% | |
| GCAAAAAGTTCAAGGTTGCA+AGG | - | chr8_2:11910651-11910670 | Msa1234570:intron | 40.0% | |
| TGGATTCCAGAGAATCACTT+TGG | - | chr8_2:11910780-11910799 | Msa1234570:intron | 40.0% | |
| TCTATTGATGTGTAGAGAGC+TGG | + | chr8_2:11910347-11910366 | None:intergenic | 40.0% | |
| CAGTTGCAGCAAAAAGTTCA+AGG | - | chr8_2:11910643-11910662 | Msa1234570:intron | 40.0% | |
| GCAAAAAGTTCAAGGTTGCA+AGG | - | chr8_2:11910651-11910670 | Msa1234570:intron | 40.0% | |
| TGGATTCCAGAGAATCACTT+TGG | - | chr8_2:11910780-11910799 | Msa1234570:intron | 40.0% | |
| GGCAGCATTAATGTTGGACA+TGG | + | chr8_2:11910288-11910307 | None:intergenic | 45.0% | |
| AGGGACAAAAACCAACATGC+AGG | - | chr8_2:11910671-11910690 | Msa1234570:intron | 45.0% | |
| GACAAAAACCAACATGCAGG+TGG | - | chr8_2:11910674-11910693 | Msa1234570:intron | 45.0% | |
| TGGATGAGAGATCCTAAGAC+TGG | - | chr8_2:11910753-11910772 | Msa1234570:intron | 45.0% | |
| GAGATCCTAAGACTGGAAAC+TGG | - | chr8_2:11910760-11910779 | Msa1234570:intron | 45.0% | |
| GGAATCCAGTTTCCAGTCTT+AGG | + | chr8_2:11910768-11910787 | None:intergenic | 45.0% | |
| ! | TCCAGAGAATCACTTTGGAG+AGG | - | chr8_2:11910785-11910804 | Msa1234570:intron | 45.0% |
| ACCTCTCCAAAGTGATTCTC+TGG | + | chr8_2:11910789-11910808 | None:intergenic | 45.0% | |
| GGCAGCATTAATGTTGGACA+TGG | + | chr8_2:11910288-11910307 | None:intergenic | 45.0% | |
| AGGGACAAAAACCAACATGC+AGG | - | chr8_2:11910671-11910690 | Msa1234570:intron | 45.0% | |
| GACAAAAACCAACATGCAGG+TGG | - | chr8_2:11910674-11910693 | Msa1234570:intron | 45.0% | |
| TGGATGAGAGATCCTAAGAC+TGG | - | chr8_2:11910753-11910772 | Msa1234570:intron | 45.0% | |
| GAGATCCTAAGACTGGAAAC+TGG | - | chr8_2:11910760-11910779 | Msa1234570:intron | 45.0% | |
| GGAATCCAGTTTCCAGTCTT+AGG | + | chr8_2:11910768-11910787 | None:intergenic | 45.0% | |
| ! | TCCAGAGAATCACTTTGGAG+AGG | - | chr8_2:11910785-11910804 | Msa1234570:intron | 45.0% |
| ACCTCTCCAAAGTGATTCTC+TGG | + | chr8_2:11910789-11910808 | None:intergenic | 45.0% | |
| !! | GTATTAAATATTTATATATA+TGG | - | chr8_2:11910601-11910620 | Msa1234570:intron | 5.0% |
| !! | GTATTAAATATTTATATATA+TGG | - | chr8_2:11910601-11910620 | Msa1234570:intron | 5.0% |
| AGTCTTCACCACCTGCATGT+TGG | + | chr8_2:11910685-11910704 | None:intergenic | 50.0% | |
| AGTCTTCACCACCTGCATGT+TGG | + | chr8_2:11910685-11910704 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_2 | gene | 11910227 | 11911015 | 11910227 | ID=Msa1234570;Name=Msa1234570 |
| chr8_2 | mRNA | 11910227 | 11911015 | 11910227 | ID=Msa1234570-mRNA-1;Parent=Msa1234570;Name=Msa1234570-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45|1|1|1|1|1|2|152|96 |
| chr8_2 | exon | 11910227 | 11910613 | 11910227 | ID=Msa1234570-mRNA-1:exon:4681;Parent=Msa1234570-mRNA-1 |
| chr8_2 | exon | 11910915 | 11911015 | 11910915 | ID=Msa1234570-mRNA-1:exon:4680;Parent=Msa1234570-mRNA-1 |
| chr8_2 | five_prime_UTR | 11910971 | 11911015 | 11910971 | ID=Msa1234570-mRNA-1:five_prime_utr;Parent=Msa1234570-mRNA-1 |
| chr8_2 | CDS | 11910915 | 11910970 | 11910915 | ID=Msa1234570-mRNA-1:cds;Parent=Msa1234570-mRNA-1 |
| chr8_2 | CDS | 11910379 | 11910613 | 11910379 | ID=Msa1234570-mRNA-1:cds;Parent=Msa1234570-mRNA-1 |
| chr8_2 | three_prime_UTR | 11910227 | 11910378 | 11910227 | ID=Msa1234570-mRNA-1:three_prime_utr;Parent=Msa1234570-mRNA-1 |
| Gene Sequence |
| Protein sequence |
