Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1315420 | XP_003627435.1 | 88.660 | 97 | 10 | 1 | 1 | 96 | 1 | 97 | 1.61e-55 | 177 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1315420 | G7L957 | 88.660 | 97 | 10 | 1 | 1 | 96 | 1 | 97 | 7.70e-56 | 177 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026450 | Msa1315420 | 0.827920 | 1.198072e-54 | -8.615850e-47 |
| Msa0653660 | Msa1315420 | 0.805025 | 1.629101e-49 | -8.615850e-47 |
| Msa0667740 | Msa1315420 | 0.804258 | 2.355191e-49 | -8.615850e-47 |
| Msa0145260 | Msa1315420 | 0.822126 | 2.797887e-53 | -8.615850e-47 |
| Msa0165520 | Msa1315420 | 0.817301 | 3.539681e-52 | -8.615850e-47 |
| Msa0195100 | Msa1315420 | 0.844477 | 7.380271e-59 | -8.615850e-47 |
| Msa0202800 | Msa1315420 | 0.852293 | 5.076284e-61 | -8.615850e-47 |
| Msa0230320 | Msa1315420 | 0.841777 | 3.867856e-58 | -8.615850e-47 |
| Msa1234570 | Msa1315420 | 0.917098 | 8.025541e-86 | -8.615850e-47 |
| Msa1315420 | Msa1339590 | 0.817955 | 2.520542e-52 | -8.615850e-47 |
| Msa1315420 | Msa1410630 | 0.843820 | 1.107850e-58 | -8.615850e-47 |
| Msa1315420 | Msa1423780 | 0.822913 | 1.836787e-53 | -8.615850e-47 |
| Msa0389770 | Msa1315420 | 0.863644 | 2.148562e-64 | -8.615850e-47 |
| Msa0461790 | Msa1315420 | 0.802166 | 6.381394e-49 | -8.615850e-47 |
| Msa1000060 | Msa1315420 | 0.813309 | 2.732538e-51 | -8.615850e-47 |
| Msa1023050 | Msa1315420 | 0.836938 | 6.976533e-57 | -8.615850e-47 |
| Msa1047800 | Msa1315420 | 0.808054 | 3.739287e-50 | -8.615850e-47 |
| Msa1091900 | Msa1315420 | 0.824818 | 6.568876e-54 | -8.615850e-47 |
| Msa0245900 | Msa1315420 | 0.809691 | 1.669696e-50 | -8.615850e-47 |
| Msa0284480 | Msa1315420 | 0.803248 | 3.815945e-49 | -8.615850e-47 |
| Msa0308630 | Msa1315420 | 0.841898 | 3.594122e-58 | -8.615850e-47 |
| Msa1175040 | Msa1315420 | 0.850906 | 1.253726e-60 | -8.615850e-47 |
| Msa1185110 | Msa1315420 | 0.804363 | 2.239283e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1315420 | MtrunA17_Chr8g0345021 | 88.660 | 97 | 10 | 1 | 1 | 96 | 1 | 97 | 1.48e-59 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTTCCTTATGAATAAA+AGG | 0.171857 | 8_4:-14843861 | Msa1315420:three_prime_UTR |
| GGAATCCAGTTTCCAGTCTT+AGG | 0.287752 | 8_4:+14844053 | None:intergenic |
| TAGACACAAACTTATTTCTA+AGG | 0.311347 | 8_4:+14843997 | None:intergenic |
| CCAAATCTCTTCTTCATATC+AGG | 0.317489 | 8_4:-14844512 | Msa1315420:intron |
| ACCTCTCCAAAGTGATTCTC+TGG | 0.376974 | 8_4:+14844032 | None:intergenic |
| CCTGATATGAAGAAGAGATT+TGG | 0.380350 | 8_4:+14844512 | None:intergenic |
| GAGATCCTAAGACTGGAAAC+TGG | 0.415942 | 8_4:-14844058 | Msa1315420:CDS |
| TAACATGAATGAATCAATAT+CGG | 0.448963 | 8_4:-14844114 | Msa1315420:CDS |
| ATAAGGAAATTCTTAAGTAA+AGG | 0.469042 | 8_4:+14843871 | None:intergenic |
| GAATTTCCTTATGAATAAAA+GGG | 0.479917 | 8_4:-14843860 | Msa1315420:three_prime_UTR |
| TGGATTCCAGAGAATCACTT+TGG | 0.493363 | 8_4:-14844038 | Msa1315420:CDS |
| AGATTTGGCAGCATTAATGT+TGG | 0.503666 | 8_4:+14844527 | None:intergenic |
| TGGATGAGAGATCCTAAGAC+TGG | 0.526718 | 8_4:-14844065 | Msa1315420:CDS |
| AGTCTTCACCACCTGCATGT+TGG | 0.574329 | 8_4:+14844136 | None:intergenic |
| TCCAGAGAATCACTTTGGAG+AGG | 0.599206 | 8_4:-14844033 | Msa1315420:CDS |
| GGCAGCATTAATGTTGGACA+TGG | 0.637036 | 8_4:+14844533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAACAAATTATCAAA+AGG | + | chr8_4:14844035-14844054 | None:intergenic | 10.0% |
| !!! | ATATTTTCCTTTAATTTTTT+TGG | - | chr8_4:14844136-14844155 | Msa1315420:CDS | 10.0% |
| !! | AATATTTAATACAATATAGA+AGG | + | chr8_4:14844191-14844210 | None:intergenic | 10.0% |
| !! | AAATTTAACAAATTATCAAA+AGG | + | chr8_4:14844035-14844054 | None:intergenic | 10.0% |
| !!! | ATATTTTCCTTTAATTTTTT+TGG | - | chr8_4:14844136-14844155 | Msa1315420:CDS | 10.0% |
| !! | AATATTTAATACAATATAGA+AGG | + | chr8_4:14844191-14844210 | None:intergenic | 10.0% |
| !!! | ATTTATCGTTGTTTTTTTAA+AGG | + | chr8_4:14843972-14843991 | None:intergenic | 15.0% |
| !! | ATTTGTTTCAAAATTGAATT+TGG | + | chr8_4:14844118-14844137 | None:intergenic | 15.0% |
| !!! | ATTTATCGTTGTTTTTTTAA+AGG | + | chr8_4:14843972-14843991 | None:intergenic | 15.0% |
| !! | ATTTGTTTCAAAATTGAATT+TGG | + | chr8_4:14844118-14844137 | None:intergenic | 15.0% |
| !!! | TTGTTTTGTACTTTTTACAA+TGG | - | chr8_4:14843851-14843870 | Msa1315420:three_prime_UTR | 20.0% |
| !!! | TTTTTATTCTCTAGAAAGAA+AGG | - | chr8_4:14844052-14844071 | Msa1315420:CDS | 20.0% |
| !! | ATGCAAACCAAAAAAATTAA+AGG | + | chr8_4:14844146-14844165 | None:intergenic | 20.0% |
| !! | TAACATGAATGAATCAATAT+CGG | - | chr8_4:14844302-14844321 | Msa1315420:intron | 20.0% |
| !!! | AAATATTTTTCTCTCTTCAT+TGG | + | chr8_4:14844331-14844350 | None:intergenic | 20.0% |
| !!! | ATGAAGAGAGAAAAATATTT+TGG | - | chr8_4:14844331-14844350 | Msa1315420:intron | 20.0% |
| !! | ATAAGGAAATTCTTAAGTAA+AGG | + | chr8_4:14844548-14844567 | None:intergenic | 20.0% |
| !! | AGAATTTCCTTATGAATAAA+AGG | - | chr8_4:14844555-14844574 | Msa1315420:exon | 20.0% |
| !! | GAATTTCCTTATGAATAAAA+GGG | - | chr8_4:14844556-14844575 | Msa1315420:exon | 20.0% |
| !!! | TTGTTTTGTACTTTTTACAA+TGG | - | chr8_4:14843851-14843870 | Msa1315420:three_prime_UTR | 20.0% |
| !!! | TTTTTATTCTCTAGAAAGAA+AGG | - | chr8_4:14844052-14844071 | Msa1315420:CDS | 20.0% |
| !! | ATGCAAACCAAAAAAATTAA+AGG | + | chr8_4:14844146-14844165 | None:intergenic | 20.0% |
| !! | TAACATGAATGAATCAATAT+CGG | - | chr8_4:14844302-14844321 | Msa1315420:intron | 20.0% |
| !!! | AAATATTTTTCTCTCTTCAT+TGG | + | chr8_4:14844331-14844350 | None:intergenic | 20.0% |
| !!! | ATGAAGAGAGAAAAATATTT+TGG | - | chr8_4:14844331-14844350 | Msa1315420:intron | 20.0% |
| !! | ATAAGGAAATTCTTAAGTAA+AGG | + | chr8_4:14844548-14844567 | None:intergenic | 20.0% |
| !! | AGAATTTCCTTATGAATAAA+AGG | - | chr8_4:14844555-14844574 | Msa1315420:exon | 20.0% |
| !! | GAATTTCCTTATGAATAAAA+GGG | - | chr8_4:14844556-14844575 | Msa1315420:exon | 20.0% |
| ! | AAAGTACAAAACAAAAAGTG+AGG | + | chr8_4:14843845-14843864 | None:intergenic | 25.0% |
| !!! | TAGACACAAACTTATTTCTA+AGG | + | chr8_4:14844422-14844441 | None:intergenic | 25.0% |
| !! | TTTGATCCCTTTTATTCATA+AGG | + | chr8_4:14844565-14844584 | None:intergenic | 25.0% |
| ! | AAAGTACAAAACAAAAAGTG+AGG | + | chr8_4:14843845-14843864 | None:intergenic | 25.0% |
| !!! | TAGACACAAACTTATTTCTA+AGG | + | chr8_4:14844422-14844441 | None:intergenic | 25.0% |
| !! | TTTGATCCCTTTTATTCATA+AGG | + | chr8_4:14844565-14844584 | None:intergenic | 25.0% |
| !!! | CAAAAAGTGAGGTTTTTTAG+AGG | + | chr8_4:14843834-14843853 | None:intergenic | 30.0% |
| AGGAAAAAAACACATGAAGA+AGG | + | chr8_4:14844171-14844190 | None:intergenic | 30.0% | |
| !!! | CAAAAAGTGAGGTTTTTTAG+AGG | + | chr8_4:14843834-14843853 | None:intergenic | 30.0% |
| AGGAAAAAAACACATGAAGA+AGG | + | chr8_4:14844171-14844190 | None:intergenic | 30.0% | |
| AGATTTGGCAGCATTAATGT+TGG | + | chr8_4:14843892-14843911 | None:intergenic | 35.0% | |
| CCTGATATGAAGAAGAGATT+TGG | + | chr8_4:14843907-14843926 | None:intergenic | 35.0% | |
| CCAAATCTCTTCTTCATATC+AGG | - | chr8_4:14843904-14843923 | Msa1315420:three_prime_UTR | 35.0% | |
| CAAAAAGTTCAAGGTTGCAA+GGG | - | chr8_4:14844250-14844269 | Msa1315420:intron | 35.0% | |
| AGATTTGGCAGCATTAATGT+TGG | + | chr8_4:14843892-14843911 | None:intergenic | 35.0% | |
| CCTGATATGAAGAAGAGATT+TGG | + | chr8_4:14843907-14843926 | None:intergenic | 35.0% | |
| CCAAATCTCTTCTTCATATC+AGG | - | chr8_4:14843904-14843923 | Msa1315420:three_prime_UTR | 35.0% | |
| CAAAAAGTTCAAGGTTGCAA+GGG | - | chr8_4:14844250-14844269 | Msa1315420:intron | 35.0% | |
| TCTATTGATGTGTAGAGAGC+TGG | + | chr8_4:14843945-14843964 | None:intergenic | 40.0% | |
| CAGTTGCAGCAAAAAGTTCA+AGG | - | chr8_4:14844241-14844260 | Msa1315420:intron | 40.0% | |
| GCAAAAAGTTCAAGGTTGCA+AGG | - | chr8_4:14844249-14844268 | Msa1315420:intron | 40.0% | |
| TGGATTCCAGAGAATCACTT+TGG | - | chr8_4:14844378-14844397 | Msa1315420:intron | 40.0% | |
| TCTATTGATGTGTAGAGAGC+TGG | + | chr8_4:14843945-14843964 | None:intergenic | 40.0% | |
| CAGTTGCAGCAAAAAGTTCA+AGG | - | chr8_4:14844241-14844260 | Msa1315420:intron | 40.0% | |
| GCAAAAAGTTCAAGGTTGCA+AGG | - | chr8_4:14844249-14844268 | Msa1315420:intron | 40.0% | |
| TGGATTCCAGAGAATCACTT+TGG | - | chr8_4:14844378-14844397 | Msa1315420:intron | 40.0% | |
| GGCAGCATTAATGTTGGACA+TGG | + | chr8_4:14843886-14843905 | None:intergenic | 45.0% | |
| AGGGACAAAAACCAACATGC+AGG | - | chr8_4:14844269-14844288 | Msa1315420:intron | 45.0% | |
| GACAAAAACCAACATGCAGG+TGG | - | chr8_4:14844272-14844291 | Msa1315420:intron | 45.0% | |
| TGGATGAGAGATCCTAAGAC+TGG | - | chr8_4:14844351-14844370 | Msa1315420:intron | 45.0% | |
| GAGATCCTAAGACTGGAAAC+TGG | - | chr8_4:14844358-14844377 | Msa1315420:intron | 45.0% | |
| GGAATCCAGTTTCCAGTCTT+AGG | + | chr8_4:14844366-14844385 | None:intergenic | 45.0% | |
| ! | TCCAGAGAATCACTTTGGAG+AGG | - | chr8_4:14844383-14844402 | Msa1315420:intron | 45.0% |
| ACCTCTCCAAAGTGATTCTC+TGG | + | chr8_4:14844387-14844406 | None:intergenic | 45.0% | |
| GGCAGCATTAATGTTGGACA+TGG | + | chr8_4:14843886-14843905 | None:intergenic | 45.0% | |
| AGGGACAAAAACCAACATGC+AGG | - | chr8_4:14844269-14844288 | Msa1315420:intron | 45.0% | |
| GACAAAAACCAACATGCAGG+TGG | - | chr8_4:14844272-14844291 | Msa1315420:intron | 45.0% | |
| TGGATGAGAGATCCTAAGAC+TGG | - | chr8_4:14844351-14844370 | Msa1315420:intron | 45.0% | |
| GAGATCCTAAGACTGGAAAC+TGG | - | chr8_4:14844358-14844377 | Msa1315420:intron | 45.0% | |
| GGAATCCAGTTTCCAGTCTT+AGG | + | chr8_4:14844366-14844385 | None:intergenic | 45.0% | |
| ! | TCCAGAGAATCACTTTGGAG+AGG | - | chr8_4:14844383-14844402 | Msa1315420:intron | 45.0% |
| ACCTCTCCAAAGTGATTCTC+TGG | + | chr8_4:14844387-14844406 | None:intergenic | 45.0% | |
| !! | GTATTAAATATTTATATATA+TGG | - | chr8_4:14844199-14844218 | Msa1315420:intron | 5.0% |
| !! | GTATTAAATATTTATATATA+TGG | - | chr8_4:14844199-14844218 | Msa1315420:intron | 5.0% |
| AGTCTTCACCACCTGCATGT+TGG | + | chr8_4:14844283-14844302 | None:intergenic | 50.0% | |
| AGTCTTCACCACCTGCATGT+TGG | + | chr8_4:14844283-14844302 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_4 | gene | 14843825 | 14844613 | 14843825 | ID=Msa1315420;Name=Msa1315420 |
| chr8_4 | mRNA | 14843825 | 14844613 | 14843825 | ID=Msa1315420-mRNA-1;Parent=Msa1315420;Name=Msa1315420-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45|1|1|1|1|1|2|152|96 |
| chr8_4 | exon | 14843825 | 14844211 | 14843825 | ID=Msa1315420-mRNA-1:exon:5644;Parent=Msa1315420-mRNA-1 |
| chr8_4 | exon | 14844513 | 14844613 | 14844513 | ID=Msa1315420-mRNA-1:exon:5643;Parent=Msa1315420-mRNA-1 |
| chr8_4 | five_prime_UTR | 14844569 | 14844613 | 14844569 | ID=Msa1315420-mRNA-1:five_prime_utr;Parent=Msa1315420-mRNA-1 |
| chr8_4 | CDS | 14844513 | 14844568 | 14844513 | ID=Msa1315420-mRNA-1:cds;Parent=Msa1315420-mRNA-1 |
| chr8_4 | CDS | 14843977 | 14844211 | 14843977 | ID=Msa1315420-mRNA-1:cds;Parent=Msa1315420-mRNA-1 |
| chr8_4 | three_prime_UTR | 14843825 | 14843976 | 14843825 | ID=Msa1315420-mRNA-1:three_prime_utr;Parent=Msa1315420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
