AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1237300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1237300 MtrunA17_Chr8g0348311 93.529 340 18 4 1 338 1 338 5.14e-179 499
Msa1237300 MtrunA17_Chr7g0248691 55.523 344 110 10 1 333 1 312 4.57e-104 308
Msa1237300 MtrunA17_Chr2g0293481 37.338 308 132 9 73 326 159 459 4.14e-48 168
Msa1237300 MtrunA17_Chr1g0202461 68.421 114 33 2 132 244 237 348 2.38e-46 161
Msa1237300 MtrunA17_Chr8g0387501 41.224 245 110 9 74 312 53 269 5.77e-41 144
Msa1237300 MtrunA17_Chr7g0270051 52.586 116 41 3 135 239 24 136 4.00e-33 123
Msa1237300 MtrunA17_Chr2g0284321 58.763 97 33 2 129 219 4 99 8.12e-33 121
Msa1237300 MtrunA17_Chr8g0379461 39.548 177 80 7 136 293 46 214 7.15e-29 111
Msa1237300 MtrunA17_Chr5g0401821 60.241 83 28 1 135 212 174 256 3.08e-28 110
Msa1237300 MtrunA17_Chr2g0319961 56.818 88 32 1 134 215 53 140 6.49e-28 109
Msa1237300 MtrunA17_Chr4g0071521 61.446 83 27 1 135 212 220 302 2.42e-27 109
Msa1237300 MtrunA17_Chr2g0289181 51.648 91 41 2 145 234 4 92 6.02e-27 106
Msa1237300 MtrunA17_Chr1g0147881 58.537 82 29 1 136 212 238 319 1.02e-26 108
Msa1237300 MtrunA17_Chr4g0053281 59.756 82 28 2 136 212 242 323 9.62e-26 105
Msa1237300 MtrunA17_Chr1g0184691 68.421 57 18 0 156 212 294 350 3.93e-23 98.6
Msa1237300 MtrunA17_Chr7g0228941 60.000 60 24 0 153 212 50 109 3.07e-22 90.5
Msa1237300 MtrunA17_Chr3g0139021 46.154 91 43 2 136 220 290 380 3.35e-21 93.6
Msa1237300 MtrunA17_Chr7g0218081 41.667 108 61 2 132 238 386 492 1.92e-20 92.0
Msa1237300 MtrunA17_Chr7g0218081 44.286 70 35 3 151 218 224 291 5.29e-11 63.5
Msa1237300 MtrunA17_Chr1g0194481 49.333 75 36 2 156 229 159 232 1.36e-19 88.2
Msa1237300 MtrunA17_Chr4g0051881 55.072 69 27 2 156 224 179 243 3.43e-19 87.0
Msa1237300 MtrunA17_Chr7g0270831 50.685 73 36 0 153 225 214 286 7.29e-19 87.0
Msa1237300 MtrunA17_Chr4g0051181 43.182 88 47 1 129 216 220 304 1.76e-18 86.3
Msa1237300 MtrunA17_Chr5g0443131 56.667 60 25 1 156 215 158 216 1.92e-18 84.7
Msa1237300 MtrunA17_Chr4g0075201 55.556 63 28 0 153 215 265 327 4.15e-18 85.1
Msa1237300 MtrunA17_Chr5g0418191 36.054 147 63 6 131 247 279 424 5.51e-18 84.3
Msa1237300 MtrunA17_Chr5g0418191 35.156 128 68 7 94 218 99 214 2.32e-11 64.7
Msa1237300 MtrunA17_Chr7g0270191 55.000 60 26 1 156 215 146 204 9.47e-18 82.4
Msa1237300 MtrunA17_Chr2g0288871 52.381 63 30 0 153 215 303 365 1.41e-17 83.6
Msa1237300 MtrunA17_Chr3g0126391 37.500 120 68 3 156 273 134 248 2.21e-17 82.0
Msa1237300 MtrunA17_Chr3g0099641 46.575 73 39 0 153 225 249 321 2.59e-17 82.8
Msa1237300 MtrunA17_Chr7g0238921 48.718 78 37 2 139 214 106 182 3.39e-17 81.3
Msa1237300 MtrunA17_Chr7g0275531 36.842 114 62 3 102 211 69 176 3.41e-17 81.3
Msa1237300 MtrunA17_Chr4g0042831 43.820 89 46 2 153 238 276 363 4.84e-17 81.3
Msa1237300 MtrunA17_Chr4g0042831 38.393 112 49 3 148 259 138 229 5.68e-15 75.1
Msa1237300 MtrunA17_Chr7g0263531 54.412 68 29 2 148 215 355 420 5.31e-17 81.6
Msa1237300 MtrunA17_Chr7g0263531 44.737 76 40 2 150 225 184 257 6.77e-14 72.4
Msa1237300 MtrunA17_Chr4g0001361 44.595 74 41 0 153 226 160 233 6.71e-17 80.1
Msa1237300 MtrunA17_Chr3g0101131 40.594 101 46 3 133 220 380 479 9.83e-17 81.3
Msa1237300 MtrunA17_Chr3g0101131 44.118 68 36 2 148 215 236 301 4.02e-11 63.9
Msa1237300 MtrunA17_Chr2g0302391 39.623 106 59 1 120 225 181 281 9.84e-17 80.9
Msa1237300 MtrunA17_Chr5g0418781 56.140 57 24 1 156 212 125 180 1.18e-16 79.0
Msa1237300 MtrunA17_Chr4g0025051 46.667 75 40 0 151 225 331 405 1.37e-16 80.9
Msa1237300 MtrunA17_Chr7g0216891 40.659 91 51 1 135 225 202 289 1.38e-16 80.9
Msa1237300 MtrunA17_Chr2g0294231 51.667 60 28 1 156 215 204 262 1.63e-16 79.7
Msa1237300 MtrunA17_Chr2g0322391 50.794 63 31 0 153 215 347 409 2.62e-16 79.7
Msa1237300 MtrunA17_Chr5g0445311 49.296 71 32 2 156 223 365 434 2.99e-16 79.7
Msa1237300 MtrunA17_Chr2g0333851 39.024 82 48 1 153 232 167 248 4.00e-16 77.8
Msa1237300 MtrunA17_Chr8g0387981 41.860 86 42 2 134 211 105 190 4.46e-16 78.2
Msa1237300 MtrunA17_Chr4g0074001 39.252 107 58 3 115 219 323 424 6.61e-16 78.6
Msa1237300 MtrunA17_Chr4g0025061 42.353 85 45 2 144 225 347 430 7.39e-16 78.6
Msa1237300 MtrunA17_Chr1g0150801 51.613 62 29 1 152 213 99 159 7.54e-16 74.3
Msa1237300 MtrunA17_Chr7g0249311 39.560 91 50 3 131 220 370 456 9.10e-16 78.2
Msa1237300 MtrunA17_Chr7g0249311 42.667 75 41 2 141 215 165 237 1.47e-12 68.6
Msa1237300 MtrunA17_Chr4g0068541 47.541 61 31 1 156 216 209 268 2.37e-15 76.6
Msa1237300 MtrunA17_Chr4g0060271 52.459 61 29 0 155 215 255 315 3.64e-15 76.6
Msa1237300 MtrunA17_Chr7g0243891 42.222 90 42 2 156 236 375 463 4.47e-15 76.3
Msa1237300 MtrunA17_Chr7g0243891 45.588 68 35 2 148 215 209 274 2.84e-12 67.4
Msa1237300 MtrunA17_Chr6g0485431 49.296 71 33 3 146 215 146 214 4.86e-15 75.1
Msa1237300 MtrunA17_Chr3g0079691 39.604 101 45 3 135 220 460 559 5.44e-15 75.9
Msa1237300 MtrunA17_Chr3g0079691 49.315 73 34 3 154 225 279 349 4.50e-12 67.0
Msa1237300 MtrunA17_Chr7g0249301 43.529 85 43 3 137 218 272 354 6.34e-15 75.1
Msa1237300 MtrunA17_Chr7g0249301 42.105 76 42 2 141 216 48 121 3.50e-13 69.7
Msa1237300 MtrunA17_Chr3g0106491 47.436 78 39 2 156 233 366 441 7.13e-15 75.5
Msa1237300 MtrunA17_Chr4g0004911 50.794 63 30 1 156 218 337 398 7.34e-15 75.5
Msa1237300 MtrunA17_Chr4g0004911 41.791 67 38 1 149 215 178 243 1.12e-11 65.5
Msa1237300 MtrunA17_Chr3g0129321 43.939 66 36 1 152 217 115 179 1.00e-14 72.0
Msa1237300 MtrunA17_Chr5g0447701 47.826 69 35 1 153 221 250 317 1.32e-14 74.3
Msa1237300 MtrunA17_Chr7g0224801 45.902 61 32 1 153 213 91 150 1.87e-14 70.5
Msa1237300 MtrunA17_Chr1g0189391 44.792 96 42 6 142 227 83 177 1.98e-14 73.2
Msa1237300 MtrunA17_Chr8g0383251 46.875 64 33 1 152 215 105 167 2.07e-14 70.9
Msa1237300 MtrunA17_Chr1g0150281 45.614 57 31 0 156 212 141 197 2.45e-14 72.4
Msa1237300 MtrunA17_Chr8g0347251 33.784 148 88 4 150 287 131 278 2.65e-14 72.8
Msa1237300 MtrunA17_Chr1g0150271 46.032 63 34 0 153 215 99 161 5.11e-14 70.9
Msa1237300 MtrunA17_Chr8g0383351 50.877 57 27 1 156 212 161 216 5.16e-14 70.5
Msa1237300 MtrunA17_Chr3g0136961 45.614 57 31 0 156 212 139 195 5.74e-14 71.2
Msa1237300 MtrunA17_Chr3g0129361 50.877 57 27 1 156 212 145 200 7.31e-14 70.1
Msa1237300 MtrunA17_Chr8g0335811 38.947 95 54 4 121 212 117 210 1.40e-13 69.3
Msa1237300 MtrunA17_Chr5g0432161 49.231 65 32 1 156 220 422 485 1.44e-13 71.6
Msa1237300 MtrunA17_Chr7g0244981 44.872 78 39 2 136 211 105 180 1.65e-13 70.1
Msa1237300 MtrunA17_Chr3g0128671 39.286 84 46 2 136 217 111 191 1.03e-12 67.8
Msa1237300 MtrunA17_Chr4g0024391 27.473 182 115 4 57 225 122 299 2.27e-12 67.8
Msa1237300 MtrunA17_Chr8g0386241 35.526 76 47 2 140 214 87 161 4.15e-12 63.9
Msa1237300 MtrunA17_Chr8g0334541 42.308 78 41 2 136 211 86 161 5.33e-12 65.5
Msa1237300 MtrunA17_Chr1g0188941 45.902 61 32 1 156 216 52 111 7.22e-12 61.6
Msa1237300 MtrunA17_Chr7g0244971 39.744 78 43 2 136 211 106 181 1.53e-11 64.3
Msa1237300 MtrunA17_Chr5g0428351 35.922 103 47 3 122 211 145 241 3.47e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1237300 AT4G01250.1 45.988 324 125 9 1 317 1 281 7.35e-78 241
Msa1237300 AT5G52830.1 57.516 153 50 4 134 283 143 283 4.73e-50 171
Msa1237300 AT4G23550.1 43.496 246 103 8 6 243 11 228 3.65e-45 157
Msa1237300 AT1G30650.1 45.581 215 85 7 136 324 188 396 5.51e-43 154
Msa1237300 AT2G34830.1 45.320 203 81 4 136 308 186 388 9.36e-42 150
Msa1237300 AT2G34830.2 45.320 203 81 4 136 308 195 397 1.26e-41 150
Msa1237300 AT5G45050.2 58.559 111 43 1 134 241 1128 1238 4.04e-34 133
Msa1237300 AT5G45050.1 58.559 111 43 1 134 241 1156 1266 4.13e-34 133
Msa1237300 AT5G45050.4 58.559 111 43 1 134 241 1017 1127 4.68e-34 133
Msa1237300 AT1G29280.1 51.282 117 40 3 122 227 31 141 7.67e-33 123
Msa1237300 AT3G58710.1 51.304 115 38 3 132 228 38 152 1.91e-30 117
Msa1237300 AT3G58710.2 52.174 115 36 4 132 228 38 151 1.27e-29 114
Msa1237300 AT2G23320.1 40.764 157 86 3 61 212 143 297 5.55e-27 108
Msa1237300 AT4G24240.1 57.831 83 30 1 135 212 256 338 6.52e-25 103
Msa1237300 AT4G31550.2 56.098 82 31 1 136 212 221 302 7.16e-25 103
Msa1237300 AT2G30590.1 55.952 84 32 1 135 213 288 371 7.21e-25 103
Msa1237300 AT4G31550.1 56.098 82 31 1 136 212 222 303 2.38e-24 101
Msa1237300 AT5G28650.1 56.627 83 31 1 135 212 237 319 7.87e-24 100
Msa1237300 AT3G04670.1 56.627 83 31 1 135 212 237 319 8.96e-24 100
Msa1237300 AT5G45260.1 53.488 86 37 2 143 226 1197 1281 6.85e-23 100
Msa1237300 AT2G24570.1 54.878 82 32 1 136 212 219 300 1.14e-22 97.1
Msa1237300 AT1G29860.1 55.072 69 29 2 156 223 137 204 8.08e-19 85.5
Msa1237300 AT5G46350.1 51.389 72 32 2 156 225 184 254 8.76e-19 86.3
Msa1237300 AT4G18170.1 56.452 62 26 1 156 217 173 233 1.20e-18 85.5
Msa1237300 AT4G04450.2 38.710 124 68 3 124 244 172 290 1.80e-18 86.3
Msa1237300 AT2G04880.2 52.703 74 29 2 153 221 281 353 2.03e-18 86.3
Msa1237300 AT2G04880.2 45.333 75 34 3 151 225 107 174 5.98e-12 67.0
Msa1237300 AT2G04880.1 52.703 74 29 2 153 221 305 377 2.39e-18 86.3
Msa1237300 AT2G04880.1 45.333 75 34 3 151 225 107 174 6.12e-12 67.0
Msa1237300 AT5G49520.1 56.667 60 25 1 156 215 222 280 2.56e-18 85.5
Msa1237300 AT3G62340.1 51.807 83 34 3 138 215 96 177 3.07e-18 84.0
Msa1237300 AT4G04450.1 43.158 95 51 1 153 244 290 384 3.28e-18 85.9
Msa1237300 AT1G62300.1 55.556 63 28 0 153 215 310 372 3.43e-18 85.9
Msa1237300 AT4G22070.4 55.556 63 28 0 153 215 295 357 5.52e-18 85.1
Msa1237300 AT4G22070.3 55.556 63 28 0 153 215 295 357 5.52e-18 85.1
Msa1237300 AT4G22070.2 55.556 63 28 0 153 215 295 357 5.52e-18 85.1
Msa1237300 AT4G22070.1 55.556 63 28 0 153 215 295 357 5.52e-18 85.1
Msa1237300 AT1G68150.1 47.368 76 40 0 155 230 235 310 9.22e-18 83.6
Msa1237300 AT5G15130.2 43.333 90 48 1 136 225 169 255 2.32e-17 83.2
Msa1237300 AT5G15130.1 43.333 90 48 1 136 225 211 297 2.60e-17 83.2
Msa1237300 AT1G13960.2 52.381 63 29 1 153 215 254 315 7.89e-17 80.9
Msa1237300 AT1G13960.2 40.789 76 43 2 140 215 61 134 1.12e-12 68.6
Msa1237300 AT1G69310.4 56.140 57 24 1 156 212 148 203 7.95e-17 80.1
Msa1237300 AT1G69310.2 56.140 57 24 1 156 212 148 203 7.95e-17 80.1
Msa1237300 AT1G69310.1 56.140 57 24 1 156 212 148 203 7.95e-17 80.1
Msa1237300 AT2G03340.1 50.000 68 33 1 153 220 413 479 1.29e-16 81.3
Msa1237300 AT2G03340.1 32.593 135 79 3 150 274 245 377 4.75e-13 70.5
Msa1237300 AT1G13960.1 52.381 63 29 1 153 215 407 468 1.64e-16 80.9
Msa1237300 AT1G13960.1 40.789 76 43 2 140 215 214 287 7.84e-13 69.7
Msa1237300 AT2G25000.2 50.000 60 30 0 156 215 104 163 1.77e-16 77.8
Msa1237300 AT2G25000.4 50.000 60 30 0 156 215 104 163 1.77e-16 77.8
Msa1237300 AT2G21900.1 49.180 61 30 1 156 216 110 169 2.18e-16 77.0
Msa1237300 AT5G26170.1 45.946 74 36 2 139 212 100 169 2.20e-16 76.3
Msa1237300 AT2G25000.3 50.000 60 30 0 156 215 126 185 3.14e-16 77.8
Msa1237300 AT2G38470.1 40.385 104 51 3 156 249 363 465 3.21e-16 79.7
Msa1237300 AT5G07100.2 51.515 66 31 1 153 218 139 203 3.22e-16 77.0
Msa1237300 AT5G07100.2 43.939 66 36 1 150 215 19 83 1.09e-11 63.9
Msa1237300 AT5G07100.4 51.515 66 31 1 153 218 139 203 3.22e-16 77.0
Msa1237300 AT5G07100.4 43.939 66 36 1 150 215 19 83 1.09e-11 63.9
Msa1237300 AT5G07100.5 51.515 66 31 1 153 218 139 203 3.22e-16 77.0
Msa1237300 AT5G07100.5 43.939 66 36 1 150 215 19 83 1.09e-11 63.9
Msa1237300 AT5G07100.3 51.515 66 31 1 153 218 139 203 3.22e-16 77.0
Msa1237300 AT5G07100.3 43.939 66 36 1 150 215 19 83 1.09e-11 63.9
Msa1237300 AT4G39410.1 41.414 99 53 3 137 231 203 300 3.65e-16 78.2
Msa1237300 AT4G01720.1 52.459 61 29 0 155 215 239 299 3.92e-16 79.7
Msa1237300 AT1G66600.1 28.571 231 116 8 87 302 31 227 4.04e-16 77.0
Msa1237300 AT2G25000.1 50.000 60 30 0 156 215 147 206 4.63e-16 77.4
Msa1237300 AT2G37260.1 50.000 86 33 4 134 212 323 405 5.24e-16 79.0
Msa1237300 AT2G37260.1 41.414 99 49 4 150 243 160 254 2.90e-11 64.7
Msa1237300 AT2G37260.2 50.000 86 33 4 134 212 241 323 5.45e-16 78.2
Msa1237300 AT2G37260.2 41.414 99 49 4 150 243 78 172 3.60e-11 63.9
Msa1237300 AT2G37260.3 50.000 86 33 4 134 212 243 325 5.64e-16 78.2
Msa1237300 AT2G37260.3 41.414 99 49 4 150 243 80 174 3.67e-11 63.9
Msa1237300 AT1G80840.1 42.254 71 41 0 156 226 147 217 5.89e-16 77.8
Msa1237300 AT1G18860.1 45.000 80 41 1 136 215 223 299 6.43e-16 79.0
Msa1237300 AT5G07100.1 51.515 66 31 1 153 218 232 296 6.85e-16 77.8
Msa1237300 AT5G07100.1 42.647 68 38 1 148 215 110 176 1.83e-11 64.7
Msa1237300 AT1G69810.1 48.611 72 36 1 155 225 203 274 7.86e-16 78.2
Msa1237300 AT4G26640.7 41.489 94 54 1 127 220 313 405 1.00e-15 78.6
Msa1237300 AT4G26640.2 41.489 94 54 1 127 220 353 445 1.08e-15 78.6
Msa1237300 AT2G47260.1 51.667 60 28 1 156 215 175 233 1.18e-15 77.4
Msa1237300 AT4G26640.1 41.489 94 54 1 127 220 281 373 1.20e-15 78.2
Msa1237300 AT1G69810.2 48.611 72 36 1 155 225 145 216 1.24e-15 77.0
Msa1237300 AT4G26640.5 41.489 94 54 1 127 220 177 269 1.25e-15 77.4
Msa1237300 AT4G26640.6 41.489 94 54 1 127 220 177 269 1.25e-15 77.4
Msa1237300 AT4G26640.4 41.489 94 54 1 127 220 177 269 1.25e-15 77.4
Msa1237300 AT4G26640.3 41.489 94 54 1 127 220 177 269 1.25e-15 77.4
Msa1237300 AT1G80590.1 29.940 167 106 3 119 274 40 206 1.78e-15 75.1
Msa1237300 AT4G11070.2 44.706 85 39 2 135 211 83 167 2.62e-15 75.5
Msa1237300 AT4G11070.1 44.706 85 39 2 135 211 115 199 3.62e-15 75.5
Msa1237300 AT4G30935.1 35.036 137 71 4 153 272 329 464 5.17e-15 76.3
Msa1237300 AT4G31800.2 46.667 60 32 0 156 215 112 171 5.28e-15 73.9
Msa1237300 AT3G01080.2 43.158 95 41 4 133 215 310 403 5.92e-15 75.9
Msa1237300 AT3G01080.2 45.205 73 38 2 151 223 201 271 3.61e-13 70.5
Msa1237300 AT4G31800.1 46.667 60 32 0 156 215 177 236 7.11e-15 74.7
Msa1237300 AT4G26440.1 47.619 84 38 3 156 234 373 455 7.29e-15 75.9
Msa1237300 AT4G26440.1 45.205 73 36 3 154 224 177 247 2.25e-12 68.2
Msa1237300 AT3G01080.1 43.158 95 41 4 133 215 272 365 8.02e-15 75.5
Msa1237300 AT3G01080.1 45.205 73 38 2 151 223 163 233 3.58e-13 70.5
Msa1237300 AT2G40740.1 43.617 94 44 3 127 211 138 231 1.26e-14 73.6
Msa1237300 AT2G40740.3 43.617 94 44 3 127 211 147 240 1.58e-14 73.6
Msa1237300 AT1G66560.1 29.493 217 118 7 87 292 31 223 1.97e-14 72.4
Msa1237300 AT2G46400.1 43.529 85 39 2 136 211 78 162 2.51e-14 72.8
Msa1237300 AT5G56270.1 50.794 63 30 1 156 218 488 549 2.52e-14 74.3
Msa1237300 AT5G56270.1 44.118 68 34 3 156 221 274 339 9.24e-11 63.5
Msa1237300 AT1G64000.1 43.056 72 40 1 156 227 115 185 2.87e-14 70.9
Msa1237300 AT5G13080.1 47.541 61 31 1 153 213 65 124 3.14e-14 69.7
Msa1237300 AT4G23810.1 42.105 95 46 3 125 211 123 216 3.45e-14 72.8
Msa1237300 AT2G40750.1 42.500 80 41 2 137 214 137 213 4.65e-14 72.8
Msa1237300 AT3G01970.1 45.000 60 32 1 153 212 63 121 6.83e-14 68.6
Msa1237300 AT2G44745.1 42.308 78 42 3 137 212 125 201 9.22e-14 70.1
Msa1237300 AT5G41570.1 43.056 72 40 1 156 227 99 169 1.85e-13 68.2
Msa1237300 AT5G43290.1 36.937 111 67 2 153 263 112 219 2.13e-13 70.1
Msa1237300 AT3G56400.1 41.026 78 41 3 136 211 104 178 2.38e-13 70.1
Msa1237300 AT3G01080.3 45.205 73 38 2 151 223 201 271 2.61e-13 70.1
Msa1237300 AT1G55600.1 44.262 61 33 1 155 215 307 366 2.96e-13 70.9
Msa1237300 AT2G30250.1 49.180 61 30 1 156 216 329 388 4.99e-13 69.7
Msa1237300 AT2G46130.1 46.774 62 32 1 153 214 28 88 5.94e-13 65.1
Msa1237300 AT1G66550.1 30.994 171 94 5 89 238 33 200 1.30e-12 67.4
Msa1237300 AT5G24110.1 50.000 58 27 1 156 211 114 171 2.41e-12 67.0
Msa1237300 AT1G66550.2 31.792 173 85 6 89 238 33 195 2.20e-11 63.5
Msa1237300 AT5G01900.1 47.368 57 28 1 156 210 111 167 5.91e-11 62.8

Find 92 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACCGACACTTTAAATTAA+AGG 0.188108 8_2:+16579700 None:intergenic
AGATATGCCTCCACTGATTT+TGG 0.224030 8_2:-16579676 Msa1237300:three_prime_UTR
GACCTTGTCTTTAACGGTTA+TGG 0.227776 8_2:-16581564 Msa1237300:exon
TTAGAGGAATTGGTACATTA+TGG 0.247413 8_2:-16579561 Msa1237300:three_prime_UTR
TAACATTATCACTATACTTT+TGG 0.253143 8_2:+16579492 None:intergenic
TTAAAAGCTAACCTTGGATA+TGG 0.255122 8_2:+16580791 None:intergenic
GGTTGTATATGAGGTGATTT+TGG 0.274712 8_2:+16581224 None:intergenic
TGAATTTGAGTTATCTGATA+TGG 0.292666 8_2:-16580015 Msa1237300:CDS
GTCAGAGAGCAAAGATGATT+TGG 0.299852 8_2:-16580069 Msa1237300:CDS
CTTTCAGACCTTGTCTTTAA+CGG 0.308252 8_2:-16581570 Msa1237300:five_prime_UTR
GGGTCGTGTCGGTGCATTAT+AGG 0.327204 8_2:+16579847 None:intergenic
TTATTAACCAGAAGCTGTTT+TGG 0.328825 8_2:+16581203 None:intergenic
TTTGTACATTACCAAAAGTA+TGG 0.353893 8_2:+16579422 None:intergenic
TAACGGTTATGGAGGTAGAT+TGG 0.366756 8_2:-16581553 Msa1237300:exon
GTGATTGCTAGCCATACTTT+TGG 0.369155 8_2:-16579433 Msa1237300:three_prime_UTR
TCATCTGGTGGTGACTGTTT+TGG 0.371167 8_2:-16579927 Msa1237300:CDS
TATACAACCAAAACAGCTTC+TGG 0.372026 8_2:-16581210 Msa1237300:CDS
ACTCACAGAAATTCTCTTGC+TGG 0.377900 8_2:-16580227 Msa1237300:CDS
ATATGGGCATGGAGGAAATA+TGG 0.384293 8_2:-16580836 Msa1237300:CDS
GGATATGGTGACCCTTTGAT+TGG 0.396288 8_2:+16580806 None:intergenic
AACGGTTATGGAGGTAGATT+GGG 0.399198 8_2:-16581552 Msa1237300:exon
GGAAGATGAGGAGGTGAGGT+TGG 0.401939 8_2:+16581502 None:intergenic
ACTTGACTTGTTTGTTCTGT+AGG 0.409675 8_2:+16581389 None:intergenic
AGAAGCTCTCATCAGACATA+TGG 0.410207 8_2:-16580853 Msa1237300:CDS
GTAACAGATGATTTCTTTGA+AGG 0.418155 8_2:-16579990 Msa1237300:CDS
AGAGCAATACTAGCAGCAAA+AGG 0.441287 8_2:+16579900 None:intergenic
TCAGTGTCCTGTCAGACATA+AGG 0.441359 8_2:+16579826 None:intergenic
GGCTAAGGTGGTTGTTGAGG+TGG 0.445368 8_2:+16581469 None:intergenic
TCTTCAGCAGTGACAGTTTG+TGG 0.449517 8_2:+16580179 None:intergenic
AAGTTCATGCAATTCTTCAA+TGG 0.450245 8_2:+16581328 None:intergenic
GATGATGATTTGAAGAAGAA+AGG 0.460344 8_2:+16581299 None:intergenic
CTTGACTTGTTTGTTCTGTA+GGG 0.462771 8_2:+16581390 None:intergenic
TGTTGAGGTGGTGGAGGAGA+AGG 0.465150 8_2:+16581481 None:intergenic
ATTATAGGTGGCAACATCAT+TGG 0.473554 8_2:+16579862 None:intergenic
TCCATTTCCTATTCTCTACA+AGG 0.475193 8_2:+16579623 None:intergenic
GAGAAGGAAGTAGAAGATAG+AGG 0.477733 8_2:+16581263 None:intergenic
GAATTTCTGTGAGTAGGAGC+AGG 0.480005 8_2:+16580236 None:intergenic
TTACTATAAGATGTTGAAGA+AGG 0.482849 8_2:+16581434 None:intergenic
TACAGATGTAGCAGTTCAAA+AGG 0.483623 8_2:-16580341 Msa1237300:CDS
GGTGGTTGTTGAGGTGGTGG+AGG 0.484588 8_2:+16581475 None:intergenic
TCCTTGTAGAGAATAGGAAA+TGG 0.485041 8_2:-16579624 Msa1237300:three_prime_UTR
AGAGAGCAAAGATGATTTGG+AGG 0.486921 8_2:-16580066 Msa1237300:CDS
GAAGGTTTAGATGAATTGAC+AGG 0.487690 8_2:-16579972 Msa1237300:CDS
AACTAACAATGCTATGTGAA+AGG 0.488846 8_2:-16579529 Msa1237300:three_prime_UTR
TAGGAAGATTCAGCATAAGA+AGG 0.490182 8_2:-16580894 Msa1237300:CDS
GTTCAAAAGGATGTTTAGCT+AGG 0.490492 8_2:-16580328 Msa1237300:CDS
TAAGGTGGTTGTTGAGGTGG+TGG 0.491931 8_2:+16581472 None:intergenic
GGTGGCTAAGGTGGTTGTTG+AGG 0.500842 8_2:+16581466 None:intergenic
ACTAACAATGCTATGTGAAA+GGG 0.509498 8_2:-16579528 Msa1237300:three_prime_UTR
ATCAGAGGCTGAAGAGGAAG+AGG 0.515216 8_2:-16580114 Msa1237300:CDS
TATGTAACTATGAACATTGA+TGG 0.516786 8_2:+16580278 None:intergenic
AGTATTGCTCTTCCTGCTTG+TGG 0.519938 8_2:-16579888 Msa1237300:CDS
CAGTGTCCTGTCAGACATAA+GGG 0.527260 8_2:+16579827 None:intergenic
GGAGGAGAAGGAAGATGAGG+AGG 0.531571 8_2:+16581493 None:intergenic
AGAAGGAAGATGAGGAGGTG+AGG 0.534411 8_2:+16581498 None:intergenic
TTCCTCTTCAGCCTCTGATG+TGG 0.535972 8_2:+16580118 None:intergenic
GAAGCTCTCATCAGACATAT+GGG 0.541248 8_2:-16580852 Msa1237300:CDS
GGTGGAGGAGAAGGAAGATG+AGG 0.548864 8_2:+16581490 None:intergenic
TGTTGATGAGGTGGCTAAGG+TGG 0.549800 8_2:+16581457 None:intergenic
AGGTGTTGATGAGGTGGCTA+AGG 0.558620 8_2:+16581454 None:intergenic
ACACCTTTAATTTAAAGTGT+CGG 0.561273 8_2:-16579703 Msa1237300:three_prime_UTR
ATCCACATCAGAGGCTGAAG+AGG 0.569315 8_2:-16580120 Msa1237300:CDS
CTCCATAACCGTTAAAGACA+AGG 0.576458 8_2:+16581562 None:intergenic
ACACGACCCTTATGTCTGAC+AGG 0.576936 8_2:-16579833 Msa1237300:three_prime_UTR
GAGCAATACTAGCAGCAAAA+GGG 0.577414 8_2:+16579901 None:intergenic
ATTGCTCTTCCTGCTTGTGG+TGG 0.580832 8_2:-16579885 Msa1237300:CDS
GAGGAATTGGTACATTATGG+AGG 0.585375 8_2:-16579558 Msa1237300:three_prime_UTR
TCACACCAAGATCCAAAAGA+AGG 0.586020 8_2:-16581154 Msa1237300:intron
CATCATTGGCCACCACAAGC+AGG 0.589284 8_2:+16579876 None:intergenic
CTTGTCTTTAACGGTTATGG+AGG 0.592755 8_2:-16581561 Msa1237300:exon
GGAAATGATGAATGATGATG+AGG 0.594118 8_2:-16580045 Msa1237300:CDS
GCAAGAGAATTTCTGTGAGT+AGG 0.601696 8_2:+16580230 None:intergenic
GTCAGACATAAGGGTCGTGT+CGG 0.602721 8_2:+16579836 None:intergenic
TAGCTAGGAAACAAGTTGAG+AGG 0.603499 8_2:-16580313 Msa1237300:CDS
TCAAAGGGTCACCATATCCA+AGG 0.606385 8_2:-16580802 Msa1237300:intron
GATCTACATGCTGTAGTCAG+AGG 0.619206 8_2:-16581530 Msa1237300:CDS
ATGTAACTATGAACATTGAT+GGG 0.626118 8_2:+16580279 None:intergenic
ATGTTCATAGTTACATACAC+TGG 0.626605 8_2:-16580272 Msa1237300:CDS
AGTTTCATCATCCACATCAG+AGG 0.630120 8_2:-16580129 Msa1237300:CDS
GTGACAGTTTGTGGTGACAA+TGG 0.637730 8_2:+16580188 None:intergenic
AATTTCTGTGAGTAGGAGCA+GGG 0.638458 8_2:+16580237 None:intergenic
TGAAGAAGGTGTTGATGAGG+TGG 0.640780 8_2:+16581448 None:intergenic
TCAGCTACCACCAAAATCAG+TGG 0.643480 8_2:+16579666 None:intergenic
TCGTGTCGGTGCATTATAGG+TGG 0.649672 8_2:+16579850 None:intergenic
GCTATGTGTCAGTGTCGTGT+CGG 0.659359 8_2:-16579751 Msa1237300:three_prime_UTR
AATGATGAATGATGATGAGG+AGG 0.660009 8_2:-16580042 Msa1237300:CDS
TCTCATCAGACATATGGGCA+TGG 0.674402 8_2:-16580847 Msa1237300:CDS
CATCAGACATATGGGCATGG+AGG 0.678030 8_2:-16580844 Msa1237300:CDS
GCTACCACCAAAATCAGTGG+AGG 0.692900 8_2:+16579669 None:intergenic
TGTTGAAGAAGGTGTTGATG+AGG 0.693091 8_2:+16581445 None:intergenic
TGATGGTGATGAATAAGAGA+AGG 0.705900 8_2:+16581247 None:intergenic
AAAACAGTAGCATCATCTGG+TGG 0.715149 8_2:-16579939 Msa1237300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCAATAAAAATCATATATTT+TGG - chr8_2:16580020-16580039 Msa1237300:CDS 10.0%
!!! TTAATTAAGTTGTTTATTTA+AGG - chr8_2:16580676-16580695 Msa1237300:intron 10.0%
!!! TCAATAAAAATCATATATTT+TGG - chr8_2:16580020-16580039 Msa1237300:CDS 10.0%
!!! TTAATTAAGTTGTTTATTTA+AGG - chr8_2:16580676-16580695 Msa1237300:intron 10.0%
!!! AATTTTCATAATCCTAAATA+TGG + chr8_2:16580065-16580084 None:intergenic 15.0%
!!! AAAGTGTTTTTTCTTTAAAT+AGG + chr8_2:16580390-16580409 None:intergenic 15.0%
!! TCAAATAAGTCAATATAAAT+AGG + chr8_2:16580438-16580457 None:intergenic 15.0%
!!! TAAAAGTTATTTCTATAAGT+TGG + chr8_2:16580649-16580668 None:intergenic 15.0%
!!! AATTTTCATAATCCTAAATA+TGG + chr8_2:16580065-16580084 None:intergenic 15.0%
!!! AAAGTGTTTTTTCTTTAAAT+AGG + chr8_2:16580390-16580409 None:intergenic 15.0%
!! TCAAATAAGTCAATATAAAT+AGG + chr8_2:16580438-16580457 None:intergenic 15.0%
!!! TAAAAGTTATTTCTATAAGT+TGG + chr8_2:16580649-16580668 None:intergenic 15.0%
!!! AACTTTTTTTAGTTTCAAAG+GGG + chr8_2:16579730-16579749 None:intergenic 20.0%
!!! GAACTTTTTTTAGTTTCAAA+GGG + chr8_2:16579731-16579750 None:intergenic 20.0%
!!! AACATTTTTTTACCTTCTTT+TGG + chr8_2:16579939-16579958 None:intergenic 20.0%
!!! GACTTAAATTTTCCATATTT+AGG - chr8_2:16580050-16580069 Msa1237300:CDS 20.0%
!! CATTTATTATTCATGATTTG+AGG - chr8_2:16580134-16580153 Msa1237300:CDS 20.0%
!!! AAATTTTTAAAAGCTAACCT+TGG + chr8_2:16580296-16580315 None:intergenic 20.0%
!!! ATCTATCAAAAAAAGTGTTT+AGG + chr8_2:16580474-16580493 None:intergenic 20.0%
!! ACAAGATAAAAATGGTTTAT+TGG + chr8_2:16580549-16580568 None:intergenic 20.0%
!! TTATGGAAACAAGATAAAAA+TGG + chr8_2:16580557-16580576 None:intergenic 20.0%
!!! TTTCTTGTTTTTGTTTTTTG+AGG - chr8_2:16581494-16581513 Msa1237300:CDS 20.0%
!!! TAACATTATCACTATACTTT+TGG + chr8_2:16581589-16581608 None:intergenic 20.0%
!!! AACTTTTTTTAGTTTCAAAG+GGG + chr8_2:16579730-16579749 None:intergenic 20.0%
!!! GAACTTTTTTTAGTTTCAAA+GGG + chr8_2:16579731-16579750 None:intergenic 20.0%
!!! AACATTTTTTTACCTTCTTT+TGG + chr8_2:16579939-16579958 None:intergenic 20.0%
!!! GACTTAAATTTTCCATATTT+AGG - chr8_2:16580050-16580069 Msa1237300:CDS 20.0%
!! CATTTATTATTCATGATTTG+AGG - chr8_2:16580134-16580153 Msa1237300:CDS 20.0%
!!! AAATTTTTAAAAGCTAACCT+TGG + chr8_2:16580296-16580315 None:intergenic 20.0%
!!! ATCTATCAAAAAAAGTGTTT+AGG + chr8_2:16580474-16580493 None:intergenic 20.0%
!! ACAAGATAAAAATGGTTTAT+TGG + chr8_2:16580549-16580568 None:intergenic 20.0%
!! TTATGGAAACAAGATAAAAA+TGG + chr8_2:16580557-16580576 None:intergenic 20.0%
!!! TTTCTTGTTTTTGTTTTTTG+AGG - chr8_2:16581494-16581513 Msa1237300:CDS 20.0%
!!! TAACATTATCACTATACTTT+TGG + chr8_2:16581589-16581608 None:intergenic 20.0%
! TTACTATAAGATGTTGAAGA+AGG + chr8_2:16579647-16579666 None:intergenic 25.0%
!!! GGAACTTTTTTTAGTTTCAA+AGG + chr8_2:16579732-16579751 None:intergenic 25.0%
!!! TGCATGTTTTTGCTAATTAT+AGG - chr8_2:16580165-16580184 Msa1237300:CDS 25.0%
! ATGTAACTATGAACATTGAT+GGG + chr8_2:16580802-16580821 None:intergenic 25.0%
! TATGTAACTATGAACATTGA+TGG + chr8_2:16580803-16580822 None:intergenic 25.0%
!!! GATGAAACTTGTTTTGTAAA+GGG + chr8_2:16580938-16580957 None:intergenic 25.0%
!!! TGATGAAACTTGTTTTGTAA+AGG + chr8_2:16580939-16580958 None:intergenic 25.0%
!! TGAATTTGAGTTATCTGATA+TGG - chr8_2:16581063-16581082 Msa1237300:intron 25.0%
!! ACACCTTTAATTTAAAGTGT+CGG - chr8_2:16581375-16581394 Msa1237300:CDS 25.0%
!!! TTTTTGTTTTTTGAGGTTAG+AGG - chr8_2:16581501-16581520 Msa1237300:CDS 25.0%
! TTTGTACATTACCAAAAGTA+TGG + chr8_2:16581659-16581678 None:intergenic 25.0%
! TTACTATAAGATGTTGAAGA+AGG + chr8_2:16579647-16579666 None:intergenic 25.0%
!!! GGAACTTTTTTTAGTTTCAA+AGG + chr8_2:16579732-16579751 None:intergenic 25.0%
!!! TGCATGTTTTTGCTAATTAT+AGG - chr8_2:16580165-16580184 Msa1237300:CDS 25.0%
! ATGTAACTATGAACATTGAT+GGG + chr8_2:16580802-16580821 None:intergenic 25.0%
! TATGTAACTATGAACATTGA+TGG + chr8_2:16580803-16580822 None:intergenic 25.0%
!!! GATGAAACTTGTTTTGTAAA+GGG + chr8_2:16580938-16580957 None:intergenic 25.0%
!!! TGATGAAACTTGTTTTGTAA+AGG + chr8_2:16580939-16580958 None:intergenic 25.0%
!! TGAATTTGAGTTATCTGATA+TGG - chr8_2:16581063-16581082 Msa1237300:intron 25.0%
!! ACACCTTTAATTTAAAGTGT+CGG - chr8_2:16581375-16581394 Msa1237300:CDS 25.0%
!!! TTTTTGTTTTTTGAGGTTAG+AGG - chr8_2:16581501-16581520 Msa1237300:CDS 25.0%
! TTTGTACATTACCAAAAGTA+TGG + chr8_2:16581659-16581678 None:intergenic 25.0%
AAGTTCATGCAATTCTTCAA+TGG + chr8_2:16579753-16579772 None:intergenic 30.0%
GATGATGATTTGAAGAAGAA+AGG + chr8_2:16579782-16579801 None:intergenic 30.0%
! TTATTAACCAGAAGCTGTTT+TGG + chr8_2:16579878-16579897 None:intergenic 30.0%
! TTTTACCTTCTTTTGGATCT+TGG + chr8_2:16579932-16579951 None:intergenic 30.0%
TATGGTCAAAAACCAATCAA+AGG - chr8_2:16580260-16580279 Msa1237300:CDS 30.0%
ATGGTCAAAAACCAATCAAA+GGG - chr8_2:16580261-16580280 Msa1237300:CDS 30.0%
TTAAAAGCTAACCTTGGATA+TGG + chr8_2:16580290-16580309 None:intergenic 30.0%
ATGTTCATAGTTACATACAC+TGG - chr8_2:16580806-16580825 Msa1237300:CDS 30.0%
!! TTGTTTTGTAAAGGGTTGTT+GGG + chr8_2:16580930-16580949 None:intergenic 30.0%
GTAACAGATGATTTCTTTGA+AGG - chr8_2:16581088-16581107 Msa1237300:intron 30.0%
!!! TTTTTTGAGGTTAGAGGAAT+TGG - chr8_2:16581507-16581526 Msa1237300:CDS 30.0%
TTAGAGGAATTGGTACATTA+TGG - chr8_2:16581517-16581536 Msa1237300:CDS 30.0%
AACTAACAATGCTATGTGAA+AGG - chr8_2:16581549-16581568 Msa1237300:exon 30.0%
ACTAACAATGCTATGTGAAA+GGG - chr8_2:16581550-16581569 Msa1237300:exon 30.0%
AAGTTCATGCAATTCTTCAA+TGG + chr8_2:16579753-16579772 None:intergenic 30.0%
GATGATGATTTGAAGAAGAA+AGG + chr8_2:16579782-16579801 None:intergenic 30.0%
! TTATTAACCAGAAGCTGTTT+TGG + chr8_2:16579878-16579897 None:intergenic 30.0%
! TTTTACCTTCTTTTGGATCT+TGG + chr8_2:16579932-16579951 None:intergenic 30.0%
TATGGTCAAAAACCAATCAA+AGG - chr8_2:16580260-16580279 Msa1237300:CDS 30.0%
ATGGTCAAAAACCAATCAAA+GGG - chr8_2:16580261-16580280 Msa1237300:CDS 30.0%
TTAAAAGCTAACCTTGGATA+TGG + chr8_2:16580290-16580309 None:intergenic 30.0%
ATGTTCATAGTTACATACAC+TGG - chr8_2:16580806-16580825 Msa1237300:CDS 30.0%
!! TTGTTTTGTAAAGGGTTGTT+GGG + chr8_2:16580930-16580949 None:intergenic 30.0%
GTAACAGATGATTTCTTTGA+AGG - chr8_2:16581088-16581107 Msa1237300:intron 30.0%
!!! TTTTTTGAGGTTAGAGGAAT+TGG - chr8_2:16581507-16581526 Msa1237300:CDS 30.0%
TTAGAGGAATTGGTACATTA+TGG - chr8_2:16581517-16581536 Msa1237300:CDS 30.0%
AACTAACAATGCTATGTGAA+AGG - chr8_2:16581549-16581568 Msa1237300:exon 30.0%
ACTAACAATGCTATGTGAAA+GGG - chr8_2:16581550-16581569 Msa1237300:exon 30.0%
CTTTCAGACCTTGTCTTTAA+CGG - chr8_2:16579508-16579527 Msa1237300:three_prime_UTR 35.0%
!! CTTGACTTGTTTGTTCTGTA+GGG + chr8_2:16579691-16579710 None:intergenic 35.0%
!! ACTTGACTTGTTTGTTCTGT+AGG + chr8_2:16579692-16579711 None:intergenic 35.0%
! TGATGGTGATGAATAAGAGA+AGG + chr8_2:16579834-16579853 None:intergenic 35.0%
!! ATATGAGGTGATTTTGGTGA+TGG + chr8_2:16579851-16579870 None:intergenic 35.0%
! GGTTGTATATGAGGTGATTT+TGG + chr8_2:16579857-16579876 None:intergenic 35.0%
!!! AGCTGTTTTGGTTGTATATG+AGG + chr8_2:16579866-16579885 None:intergenic 35.0%
TATACAACCAAAACAGCTTC+TGG - chr8_2:16579868-16579887 Msa1237300:exon 35.0%
TAGGAAGATTCAGCATAAGA+AGG - chr8_2:16580184-16580203 Msa1237300:CDS 35.0%
!! GTTGTTTTCATTAGCTATCC+TGG + chr8_2:16580585-16580604 None:intergenic 35.0%
TACAGATGTAGCAGTTCAAA+AGG - chr8_2:16580737-16580756 Msa1237300:intron 35.0%
GTTCAAAAGGATGTTTAGCT+AGG - chr8_2:16580750-16580769 Msa1237300:intron 35.0%
!!! CTTGTTTTGTAAAGGGTTGT+TGG + chr8_2:16580931-16580950 None:intergenic 35.0%
! GGAAATGATGAATGATGATG+AGG - chr8_2:16581033-16581052 Msa1237300:intron 35.0%
! AATGATGAATGATGATGAGG+AGG - chr8_2:16581036-16581055 Msa1237300:intron 35.0%
GAAGGTTTAGATGAATTGAC+AGG - chr8_2:16581106-16581125 Msa1237300:intron 35.0%
ATTATAGGTGGCAACATCAT+TGG + chr8_2:16581219-16581238 None:intergenic 35.0%
! GCACCGACACTTTAAATTAA+AGG + chr8_2:16581381-16581400 None:intergenic 35.0%
! AGCTTTTCCTTGTAGAGAAT+AGG - chr8_2:16581448-16581467 Msa1237300:CDS 35.0%
TCCTTGTAGAGAATAGGAAA+TGG - chr8_2:16581454-16581473 Msa1237300:CDS 35.0%
TCCATTTCCTATTCTCTACA+AGG + chr8_2:16581458-16581477 None:intergenic 35.0%
CTTTCAGACCTTGTCTTTAA+CGG - chr8_2:16579508-16579527 Msa1237300:three_prime_UTR 35.0%
!! CTTGACTTGTTTGTTCTGTA+GGG + chr8_2:16579691-16579710 None:intergenic 35.0%
!! ACTTGACTTGTTTGTTCTGT+AGG + chr8_2:16579692-16579711 None:intergenic 35.0%
! TGATGGTGATGAATAAGAGA+AGG + chr8_2:16579834-16579853 None:intergenic 35.0%
!! ATATGAGGTGATTTTGGTGA+TGG + chr8_2:16579851-16579870 None:intergenic 35.0%
! GGTTGTATATGAGGTGATTT+TGG + chr8_2:16579857-16579876 None:intergenic 35.0%
!!! AGCTGTTTTGGTTGTATATG+AGG + chr8_2:16579866-16579885 None:intergenic 35.0%
TATACAACCAAAACAGCTTC+TGG - chr8_2:16579868-16579887 Msa1237300:exon 35.0%
TAGGAAGATTCAGCATAAGA+AGG - chr8_2:16580184-16580203 Msa1237300:CDS 35.0%
!! GTTGTTTTCATTAGCTATCC+TGG + chr8_2:16580585-16580604 None:intergenic 35.0%
TACAGATGTAGCAGTTCAAA+AGG - chr8_2:16580737-16580756 Msa1237300:intron 35.0%
GTTCAAAAGGATGTTTAGCT+AGG - chr8_2:16580750-16580769 Msa1237300:intron 35.0%
!!! CTTGTTTTGTAAAGGGTTGT+TGG + chr8_2:16580931-16580950 None:intergenic 35.0%
! GGAAATGATGAATGATGATG+AGG - chr8_2:16581033-16581052 Msa1237300:intron 35.0%
! AATGATGAATGATGATGAGG+AGG - chr8_2:16581036-16581055 Msa1237300:intron 35.0%
GAAGGTTTAGATGAATTGAC+AGG - chr8_2:16581106-16581125 Msa1237300:intron 35.0%
ATTATAGGTGGCAACATCAT+TGG + chr8_2:16581219-16581238 None:intergenic 35.0%
! GCACCGACACTTTAAATTAA+AGG + chr8_2:16581381-16581400 None:intergenic 35.0%
! AGCTTTTCCTTGTAGAGAAT+AGG - chr8_2:16581448-16581467 Msa1237300:CDS 35.0%
TCCTTGTAGAGAATAGGAAA+TGG - chr8_2:16581454-16581473 Msa1237300:CDS 35.0%
TCCATTTCCTATTCTCTACA+AGG + chr8_2:16581458-16581477 None:intergenic 35.0%
GACCTTGTCTTTAACGGTTA+TGG - chr8_2:16579514-16579533 Msa1237300:three_prime_UTR 40.0%
CTCCATAACCGTTAAAGACA+AGG + chr8_2:16579519-16579538 None:intergenic 40.0%
CTTGTCTTTAACGGTTATGG+AGG - chr8_2:16579517-16579536 Msa1237300:three_prime_UTR 40.0%
TAACGGTTATGGAGGTAGAT+TGG - chr8_2:16579525-16579544 Msa1237300:three_prime_UTR 40.0%
AACGGTTATGGAGGTAGATT+GGG - chr8_2:16579526-16579545 Msa1237300:three_prime_UTR 40.0%
!! TGTTGAAGAAGGTGTTGATG+AGG + chr8_2:16579636-16579655 None:intergenic 40.0%
GAGAAGGAAGTAGAAGATAG+AGG + chr8_2:16579818-16579837 None:intergenic 40.0%
!! TCTTTTGGATCTTGGTGTGA+TGG + chr8_2:16579924-16579943 None:intergenic 40.0%
TCACACCAAGATCCAAAAGA+AGG - chr8_2:16579924-16579943 Msa1237300:CDS 40.0%
AGAAGCTCTCATCAGACATA+TGG - chr8_2:16580225-16580244 Msa1237300:CDS 40.0%
GAAGCTCTCATCAGACATAT+GGG - chr8_2:16580226-16580245 Msa1237300:CDS 40.0%
ATATGGGCATGGAGGAAATA+TGG - chr8_2:16580242-16580261 Msa1237300:CDS 40.0%
TTGTGTCTGTGTGATTCACA+GGG - chr8_2:16580709-16580728 Msa1237300:intron 40.0%
! TAGCTAGGAAACAAGTTGAG+AGG - chr8_2:16580765-16580784 Msa1237300:intron 40.0%
AATTTCTGTGAGTAGGAGCA+GGG + chr8_2:16580844-16580863 None:intergenic 40.0%
GCAAGAGAATTTCTGTGAGT+AGG + chr8_2:16580851-16580870 None:intergenic 40.0%
ACTCACAGAAATTCTCTTGC+TGG - chr8_2:16580851-16580870 Msa1237300:CDS 40.0%
AGTTTCATCATCCACATCAG+AGG - chr8_2:16580949-16580968 Msa1237300:intron 40.0%
GTCAGAGAGCAAAGATGATT+TGG - chr8_2:16581009-16581028 Msa1237300:intron 40.0%
! AGAGAGCAAAGATGATTTGG+AGG - chr8_2:16581012-16581031 Msa1237300:intron 40.0%
! GATGAATTGACAGGTTTTGC+TGG - chr8_2:16581115-16581134 Msa1237300:intron 40.0%
GGAAAAACAGTAGCATCATC+TGG - chr8_2:16581136-16581155 Msa1237300:intron 40.0%
AAAACAGTAGCATCATCTGG+TGG - chr8_2:16581139-16581158 Msa1237300:intron 40.0%
GAGCAATACTAGCAGCAAAA+GGG + chr8_2:16581180-16581199 None:intergenic 40.0%
AGAGCAATACTAGCAGCAAA+AGG + chr8_2:16581181-16581200 None:intergenic 40.0%
!!! AGATATGCCTCCACTGATTT+TGG - chr8_2:16581402-16581421 Msa1237300:CDS 40.0%
GAGGAATTGGTACATTATGG+AGG - chr8_2:16581520-16581539 Msa1237300:CDS 40.0%
! GTGATTGCTAGCCATACTTT+TGG - chr8_2:16581645-16581664 Msa1237300:five_prime_UTR 40.0%
GACCTTGTCTTTAACGGTTA+TGG - chr8_2:16579514-16579533 Msa1237300:three_prime_UTR 40.0%
CTCCATAACCGTTAAAGACA+AGG + chr8_2:16579519-16579538 None:intergenic 40.0%
CTTGTCTTTAACGGTTATGG+AGG - chr8_2:16579517-16579536 Msa1237300:three_prime_UTR 40.0%
TAACGGTTATGGAGGTAGAT+TGG - chr8_2:16579525-16579544 Msa1237300:three_prime_UTR 40.0%
AACGGTTATGGAGGTAGATT+GGG - chr8_2:16579526-16579545 Msa1237300:three_prime_UTR 40.0%
!! TGTTGAAGAAGGTGTTGATG+AGG + chr8_2:16579636-16579655 None:intergenic 40.0%
GAGAAGGAAGTAGAAGATAG+AGG + chr8_2:16579818-16579837 None:intergenic 40.0%
!! TCTTTTGGATCTTGGTGTGA+TGG + chr8_2:16579924-16579943 None:intergenic 40.0%
TCACACCAAGATCCAAAAGA+AGG - chr8_2:16579924-16579943 Msa1237300:CDS 40.0%
AGAAGCTCTCATCAGACATA+TGG - chr8_2:16580225-16580244 Msa1237300:CDS 40.0%
GAAGCTCTCATCAGACATAT+GGG - chr8_2:16580226-16580245 Msa1237300:CDS 40.0%
ATATGGGCATGGAGGAAATA+TGG - chr8_2:16580242-16580261 Msa1237300:CDS 40.0%
TTGTGTCTGTGTGATTCACA+GGG - chr8_2:16580709-16580728 Msa1237300:intron 40.0%
! TAGCTAGGAAACAAGTTGAG+AGG - chr8_2:16580765-16580784 Msa1237300:intron 40.0%
AATTTCTGTGAGTAGGAGCA+GGG + chr8_2:16580844-16580863 None:intergenic 40.0%
GCAAGAGAATTTCTGTGAGT+AGG + chr8_2:16580851-16580870 None:intergenic 40.0%
ACTCACAGAAATTCTCTTGC+TGG - chr8_2:16580851-16580870 Msa1237300:CDS 40.0%
AGTTTCATCATCCACATCAG+AGG - chr8_2:16580949-16580968 Msa1237300:intron 40.0%
GTCAGAGAGCAAAGATGATT+TGG - chr8_2:16581009-16581028 Msa1237300:intron 40.0%
! AGAGAGCAAAGATGATTTGG+AGG - chr8_2:16581012-16581031 Msa1237300:intron 40.0%
! GATGAATTGACAGGTTTTGC+TGG - chr8_2:16581115-16581134 Msa1237300:intron 40.0%
GGAAAAACAGTAGCATCATC+TGG - chr8_2:16581136-16581155 Msa1237300:intron 40.0%
AAAACAGTAGCATCATCTGG+TGG - chr8_2:16581139-16581158 Msa1237300:intron 40.0%
GAGCAATACTAGCAGCAAAA+GGG + chr8_2:16581180-16581199 None:intergenic 40.0%
AGAGCAATACTAGCAGCAAA+AGG + chr8_2:16581181-16581200 None:intergenic 40.0%
!!! AGATATGCCTCCACTGATTT+TGG - chr8_2:16581402-16581421 Msa1237300:CDS 40.0%
GAGGAATTGGTACATTATGG+AGG - chr8_2:16581520-16581539 Msa1237300:CDS 40.0%
! GTGATTGCTAGCCATACTTT+TGG - chr8_2:16581645-16581664 Msa1237300:five_prime_UTR 40.0%
GATCTACATGCTGTAGTCAG+AGG - chr8_2:16579548-16579567 Msa1237300:three_prime_UTR 45.0%
!! TGAAGAAGGTGTTGATGAGG+TGG + chr8_2:16579633-16579652 None:intergenic 45.0%
TCTCATCAGACATATGGGCA+TGG - chr8_2:16580231-16580250 Msa1237300:CDS 45.0%
GGATATGGTGACCCTTTGAT+TGG + chr8_2:16580275-16580294 None:intergenic 45.0%
TCAAAGGGTCACCATATCCA+AGG - chr8_2:16580276-16580295 Msa1237300:CDS 45.0%
CTTGTTTCCATAAGCTCTCC+AGG - chr8_2:16580564-16580583 Msa1237300:intron 45.0%
TAGCTATCCTGGAGAGCTTA+TGG + chr8_2:16580574-16580593 None:intergenic 45.0%
CTTGTGTCTGTGTGATTCAC+AGG - chr8_2:16580708-16580727 Msa1237300:intron 45.0%
TGTGTCTGTGTGATTCACAG+GGG - chr8_2:16580710-16580729 Msa1237300:intron 45.0%
GAATTTCTGTGAGTAGGAGC+AGG + chr8_2:16580845-16580864 None:intergenic 45.0%
GTGACAGTTTGTGGTGACAA+TGG + chr8_2:16580893-16580912 None:intergenic 45.0%
TCTTCAGCAGTGACAGTTTG+TGG + chr8_2:16580902-16580921 None:intergenic 45.0%
!! TCATCTGGTGGTGACTGTTT+TGG - chr8_2:16581151-16581170 Msa1237300:intron 45.0%
AGTATTGCTCTTCCTGCTTG+TGG - chr8_2:16581190-16581209 Msa1237300:CDS 45.0%
CAGTGTCCTGTCAGACATAA+GGG + chr8_2:16581254-16581273 None:intergenic 45.0%
TCAGTGTCCTGTCAGACATA+AGG + chr8_2:16581255-16581274 None:intergenic 45.0%
!!! TATGCCTCCACTGATTTTGG+TGG - chr8_2:16581405-16581424 Msa1237300:CDS 45.0%
TCAGCTACCACCAAAATCAG+TGG + chr8_2:16581415-16581434 None:intergenic 45.0%
GATCTACATGCTGTAGTCAG+AGG - chr8_2:16579548-16579567 Msa1237300:three_prime_UTR 45.0%
!! TGAAGAAGGTGTTGATGAGG+TGG + chr8_2:16579633-16579652 None:intergenic 45.0%
TCTCATCAGACATATGGGCA+TGG - chr8_2:16580231-16580250 Msa1237300:CDS 45.0%
GGATATGGTGACCCTTTGAT+TGG + chr8_2:16580275-16580294 None:intergenic 45.0%
TCAAAGGGTCACCATATCCA+AGG - chr8_2:16580276-16580295 Msa1237300:CDS 45.0%
CTTGTTTCCATAAGCTCTCC+AGG - chr8_2:16580564-16580583 Msa1237300:intron 45.0%
TAGCTATCCTGGAGAGCTTA+TGG + chr8_2:16580574-16580593 None:intergenic 45.0%
CTTGTGTCTGTGTGATTCAC+AGG - chr8_2:16580708-16580727 Msa1237300:intron 45.0%
TGTGTCTGTGTGATTCACAG+GGG - chr8_2:16580710-16580729 Msa1237300:intron 45.0%
GAATTTCTGTGAGTAGGAGC+AGG + chr8_2:16580845-16580864 None:intergenic 45.0%
GTGACAGTTTGTGGTGACAA+TGG + chr8_2:16580893-16580912 None:intergenic 45.0%
TCTTCAGCAGTGACAGTTTG+TGG + chr8_2:16580902-16580921 None:intergenic 45.0%
!! TCATCTGGTGGTGACTGTTT+TGG - chr8_2:16581151-16581170 Msa1237300:intron 45.0%
AGTATTGCTCTTCCTGCTTG+TGG - chr8_2:16581190-16581209 Msa1237300:CDS 45.0%
CAGTGTCCTGTCAGACATAA+GGG + chr8_2:16581254-16581273 None:intergenic 45.0%
TCAGTGTCCTGTCAGACATA+AGG + chr8_2:16581255-16581274 None:intergenic 45.0%
!!! TATGCCTCCACTGATTTTGG+TGG - chr8_2:16581405-16581424 Msa1237300:CDS 45.0%
TCAGCTACCACCAAAATCAG+TGG + chr8_2:16581415-16581434 None:intergenic 45.0%
!!! TTAAATTTGATAAATATTTT+TGG + chr8_2:16580333-16580352 None:intergenic 5.0%
!!! TTAAATTTGATAAATATTTT+TGG + chr8_2:16580333-16580352 None:intergenic 5.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr8_2:16579450-16579469 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr8_2:16579449-16579468 None:intergenic 50.0%
AGAAGGAAGATGAGGAGGTG+AGG + chr8_2:16579583-16579602 None:intergenic 50.0%
TAAGGTGGTTGTTGAGGTGG+TGG + chr8_2:16579609-16579628 None:intergenic 50.0%
! TGTTGATGAGGTGGCTAAGG+TGG + chr8_2:16579624-16579643 None:intergenic 50.0%
!! AGGTGTTGATGAGGTGGCTA+AGG + chr8_2:16579627-16579646 None:intergenic 50.0%
CATCAGACATATGGGCATGG+AGG - chr8_2:16580234-16580253 Msa1237300:CDS 50.0%
ATCCACATCAGAGGCTGAAG+AGG - chr8_2:16580958-16580977 Msa1237300:intron 50.0%
TTCCTCTTCAGCCTCTGATG+TGG + chr8_2:16580963-16580982 None:intergenic 50.0%
ATCAGAGGCTGAAGAGGAAG+AGG - chr8_2:16580964-16580983 Msa1237300:intron 50.0%
ATTGCTCTTCCTGCTTGTGG+TGG - chr8_2:16581193-16581212 Msa1237300:CDS 50.0%
! TCGTGTCGGTGCATTATAGG+TGG + chr8_2:16581231-16581250 None:intergenic 50.0%
GTCAGACATAAGGGTCGTGT+CGG + chr8_2:16581245-16581264 None:intergenic 50.0%
ACACGACCCTTATGTCTGAC+AGG - chr8_2:16581245-16581264 Msa1237300:CDS 50.0%
GCTATGTGTCAGTGTCGTGT+CGG - chr8_2:16581327-16581346 Msa1237300:CDS 50.0%
GCTACCACCAAAATCAGTGG+AGG + chr8_2:16581412-16581431 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr8_2:16579450-16579469 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr8_2:16579449-16579468 None:intergenic 50.0%
AGAAGGAAGATGAGGAGGTG+AGG + chr8_2:16579583-16579602 None:intergenic 50.0%
TAAGGTGGTTGTTGAGGTGG+TGG + chr8_2:16579609-16579628 None:intergenic 50.0%
! TGTTGATGAGGTGGCTAAGG+TGG + chr8_2:16579624-16579643 None:intergenic 50.0%
!! AGGTGTTGATGAGGTGGCTA+AGG + chr8_2:16579627-16579646 None:intergenic 50.0%
CATCAGACATATGGGCATGG+AGG - chr8_2:16580234-16580253 Msa1237300:CDS 50.0%
ATCCACATCAGAGGCTGAAG+AGG - chr8_2:16580958-16580977 Msa1237300:intron 50.0%
TTCCTCTTCAGCCTCTGATG+TGG + chr8_2:16580963-16580982 None:intergenic 50.0%
ATCAGAGGCTGAAGAGGAAG+AGG - chr8_2:16580964-16580983 Msa1237300:intron 50.0%
ATTGCTCTTCCTGCTTGTGG+TGG - chr8_2:16581193-16581212 Msa1237300:CDS 50.0%
! TCGTGTCGGTGCATTATAGG+TGG + chr8_2:16581231-16581250 None:intergenic 50.0%
GTCAGACATAAGGGTCGTGT+CGG + chr8_2:16581245-16581264 None:intergenic 50.0%
ACACGACCCTTATGTCTGAC+AGG - chr8_2:16581245-16581264 Msa1237300:CDS 50.0%
GCTATGTGTCAGTGTCGTGT+CGG - chr8_2:16581327-16581346 Msa1237300:CDS 50.0%
GCTACCACCAAAATCAGTGG+AGG + chr8_2:16581412-16581431 None:intergenic 50.0%
!!! AGAGAGGGCGCTGACTTTTG+AGG + chr8_2:16579435-16579454 None:intergenic 55.0%
GGAAGATGAGGAGGTGAGGT+TGG + chr8_2:16579579-16579598 None:intergenic 55.0%
GGAGGAGAAGGAAGATGAGG+AGG + chr8_2:16579588-16579607 None:intergenic 55.0%
GGTGGAGGAGAAGGAAGATG+AGG + chr8_2:16579591-16579610 None:intergenic 55.0%
!! TGTTGAGGTGGTGGAGGAGA+AGG + chr8_2:16579600-16579619 None:intergenic 55.0%
GGCTAAGGTGGTTGTTGAGG+TGG + chr8_2:16579612-16579631 None:intergenic 55.0%
GGTGGCTAAGGTGGTTGTTG+AGG + chr8_2:16579615-16579634 None:intergenic 55.0%
CATCATTGGCCACCACAAGC+AGG + chr8_2:16581205-16581224 None:intergenic 55.0%
! GGGTCGTGTCGGTGCATTAT+AGG + chr8_2:16581234-16581253 None:intergenic 55.0%
!!! AGAGAGGGCGCTGACTTTTG+AGG + chr8_2:16579435-16579454 None:intergenic 55.0%
GGAAGATGAGGAGGTGAGGT+TGG + chr8_2:16579579-16579598 None:intergenic 55.0%
GGAGGAGAAGGAAGATGAGG+AGG + chr8_2:16579588-16579607 None:intergenic 55.0%
GGTGGAGGAGAAGGAAGATG+AGG + chr8_2:16579591-16579610 None:intergenic 55.0%
!! TGTTGAGGTGGTGGAGGAGA+AGG + chr8_2:16579600-16579619 None:intergenic 55.0%
GGCTAAGGTGGTTGTTGAGG+TGG + chr8_2:16579612-16579631 None:intergenic 55.0%
GGTGGCTAAGGTGGTTGTTG+AGG + chr8_2:16579615-16579634 None:intergenic 55.0%
CATCATTGGCCACCACAAGC+AGG + chr8_2:16581205-16581224 None:intergenic 55.0%
! GGGTCGTGTCGGTGCATTAT+AGG + chr8_2:16581234-16581253 None:intergenic 55.0%
! GGTGGTTGTTGAGGTGGTGG+AGG + chr8_2:16579606-16579625 None:intergenic 60.0%
! GGTGGTTGTTGAGGTGGTGG+AGG + chr8_2:16579606-16579625 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 16579410 16581690 16579410 ID=Msa1237300;Name=Msa1237300
chr8_2 mRNA 16579410 16581690 16579410 ID=Msa1237300-mRNA-1;Parent=Msa1237300;Name=Msa1237300-mRNA-1;_AED=0.01;_eAED=0.01;_QI=123|1|1|1|1|1|3|468|338
chr8_2 exon 16579410 16580370 16579410 ID=Msa1237300-mRNA-1:exon:6223;Parent=Msa1237300-mRNA-1
chr8_2 exon 16580803 16580913 16580803 ID=Msa1237300-mRNA-1:exon:6222;Parent=Msa1237300-mRNA-1
chr8_2 exon 16581155 16581690 16581155 ID=Msa1237300-mRNA-1:exon:6221;Parent=Msa1237300-mRNA-1
chr8_2 five_prime_UTR 16581568 16581690 16581568 ID=Msa1237300-mRNA-1:five_prime_utr;Parent=Msa1237300-mRNA-1
chr8_2 CDS 16581155 16581567 16581155 ID=Msa1237300-mRNA-1:cds;Parent=Msa1237300-mRNA-1
chr8_2 CDS 16580803 16580913 16580803 ID=Msa1237300-mRNA-1:cds;Parent=Msa1237300-mRNA-1
chr8_2 CDS 16579878 16580370 16579878 ID=Msa1237300-mRNA-1:cds;Parent=Msa1237300-mRNA-1
chr8_2 three_prime_UTR 16579410 16579877 16579410 ID=Msa1237300-mRNA-1:three_prime_utr;Parent=Msa1237300-mRNA-1
Gene Sequence

>Msa1237300

ATGGAGGTAGATTGGGATCTACATGCTGTAGTCAGAGGCTATACCAACCTCACCTCCTCATCTTCCTTCTCCTCCACCACCTCAACAACCACCTTAGCCACCTCATCAACACCTTCTTCAACATCTTATAGTAATAATAACTTTTTCTCTATTTACCCTACAGAACAAACAAGTCAAGTTTTTTCACTTTCAAACCCCTTTGAAACTAAAAAAAGTTCCATTGAAGAATTGCATGAACTTTGCAAACCTTTCTTCTTCAAATCATCATCATCATCATCACAACCTCTATCTTCTACTTCCTTCTCTTATTCATCACCATCACCAAAATCACCTCATATACAACCAAAACAGCTTCTGGTTAATAACAAGCTTCATCATGCTGCTTCTGCCATCACACCAAGATCCAAAAGAAGGAAGATTCAGCATAAGAAGGTTTGTGAAGTGCAAGCTGAGAAGCTCTCATCAGACATATGGGCATGGAGGAAATATGGTCAAAAACCAATCAAAGGGTCACCATATCCAAGGGGATATTACAGATGTAGCAGTTCAAAAGGATGTTTAGCTAGGAAACAAGTTGAGAGGAACAAATCAGACCCATCAATGTTCATAGTTACATACACTGGTGAACATAGTCACCCTGCTCCTACTCACAGAAATTCTCTTGCTGGTTCAACAAGACAGAAGCCATTGTCACCACAAACTGTCACTGCTGAAGATTCTTCCCAACAACCCTTTACAAAACAAGTTTCATCATCCACATCAGAGGCTGAAGAGGAAGAGGCTACAACACTCTTAAGTGTAAAGTCAGAGAGCAAAGATGATTTGGAGGAAATGATGAATGATGATGAGGAGGAAAATGAATTTGAGTTATCTGATATGGTTGTAACAGATGATTTCTTTGAAGGTTTAGATGAATTGACAGGTTTTGCTGGAAAAACAGTAGCATCATCTGGTGGTGACTGTTTTGGTGACCCTTTTGCTGCTAGTATTGCTCTTCCTGCTTGTGGTGGCCAATGA
Protein sequence

>Msa1237300

MEVDWDLHAVVRGYTNLTSSSSFSSTTSTTTLATSSTPSSTSYSNNNFFSIYPTEQTSQVFSLSNPFETKKSSIEELHELCKPFFFKSSSSSSQPLSSTSFSYSSPSPKSPHIQPKQLLVNNKLHHAASAITPRSKRRKIQHKKVCEVQAEKLSSDIWAWRKYGQKPIKGSPYPRGYYRCSSSKGCLARKQVERNKSDPSMFIVTYTGEHSHPAPTHRNSLAGSTRQKPLSPQTVTAEDSSQQPFTKQVSSSTSEAEEEEATTLLSVKSESKDDLEEMMNDDEEENEFELSDMVVTDDFFEGLDELTGFAGKTVASSGGDCFGDPFAASIALPACGGQ*