AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1239540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239540 MtrunA17_Chr8g0351241 99.531 213 1 0 1 213 1 213 1.20e-160 442
Msa1239540 MtrunA17_Chr7g0246291 57.653 196 80 2 9 203 5 198 8.80e-81 239
Msa1239540 MtrunA17_Chr7g0246611 57.653 196 80 2 9 203 5 198 8.80e-81 239
Msa1239540 MtrunA17_Chr7g0246621 56.938 209 77 3 1 205 1 200 4.32e-78 233
Msa1239540 MtrunA17_Chr7g0246261 58.173 208 74 4 1 204 1 199 1.37e-77 231
Msa1239540 MtrunA17_Chr7g0246281 57.812 192 76 2 15 205 1 188 3.67e-73 220
Msa1239540 MtrunA17_Chr7g0246601 59.231 130 47 2 76 205 5 128 7.27e-47 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1239540 AT3G18030.1 78.894 199 41 1 13 211 9 206 5.86e-124 349
Msa1239540 AT1G48605.1 76.923 195 44 1 14 208 2 195 6.40e-118 333

Find 83 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAACAACTTTGATCTT+AGG 0.238461 8_2:-21353007 Msa1239540:three_prime_UTR
CCAACTGAACTCACAGGTTC+TGG 0.249432 8_2:+21354970 None:intergenic
AGGTTAGCTTGTGGGGATTA+TGG 0.262541 8_2:-21353234 Msa1239540:CDS
GAGCCTTACAGTAGAGTAAA+TGG 0.285370 8_2:+21353185 None:intergenic
ATTATAAGAGGGAAGACTTT+TGG 0.296026 8_2:+21356369 None:intergenic
GAGTAAAATCCGAGGCTTCC+TGG 0.300240 8_2:+21354918 None:intergenic
TTGGCCCGGTGGTATTGGTT+TGG 0.321281 8_2:+21356388 None:intergenic
GTTCCACATAAAAGTGTTCA+TGG 0.333551 8_2:+21353329 None:intergenic
CTCACCCTCTGAGACATAAA+AGG 0.344105 8_2:+21352948 None:intergenic
TGGCCCGGTGGTATTGGTTT+GGG 0.345500 8_2:+21356389 None:intergenic
GGTTTGTATACACACCCTTT+TGG 0.357670 8_2:-21352926 Msa1239540:three_prime_UTR
AAGATCTTTGTTGAAATCTC+TGG 0.366216 8_2:-21355008 Msa1239540:five_prime_UTR
TTTGTCATTGTTTCACTGAA+TGG 0.370610 8_2:-21354855 Msa1239540:CDS
TGTTTGCTGTTGTAGATTGC+TGG 0.375544 8_2:-21353426 Msa1239540:intron
TCACATGTATCGTGCGAGCC+TGG 0.385273 8_2:-21353383 Msa1239540:CDS
CAAGCTAACCTCTTTGTAAC+AGG 0.386122 8_2:+21353246 None:intergenic
GTGGTGTGTAATAGTGTAAA+TGG 0.407317 8_2:-21352985 Msa1239540:three_prime_UTR
AAAATAGCATCGACTTATCA+AGG 0.416965 8_2:+21353068 None:intergenic
AGTAAAATCCGAGGCTTCCT+GGG 0.419242 8_2:+21354919 None:intergenic
TTGTCATTGTTTCACTGAAT+GGG 0.424119 8_2:-21354854 Msa1239540:CDS
TTCACATTGAGCTTCGCAAA+TGG 0.429618 8_2:-21354702 Msa1239540:CDS
TACACGACCAAATATCCAAA+AGG 0.439002 8_2:+21352911 None:intergenic
TAAATATCCACCATTCAAAC+AGG 0.444612 8_2:+21355030 None:intergenic
CGTATACAAAATCACATCCT+TGG 0.455438 8_2:+21354771 None:intergenic
TGTATACGGACGACGATGAA+TGG 0.456117 8_2:-21354756 Msa1239540:CDS
CAACTGAACTCACAGGTTCT+GGG 0.458227 8_2:+21354971 None:intergenic
TTGTTTCCTTGTTTGTAGAA+TGG 0.460477 8_2:-21352853 Msa1239540:three_prime_UTR
CCATTGTCGGCAAACACTCT+TGG 0.468148 8_2:-21354658 Msa1239540:CDS
CAAAACACGACACACGAGAC+GGG 0.469256 8_2:-21356288 Msa1239540:five_prime_UTR
TTACAAAGAGGTTAGCTTGT+GGG 0.472912 8_2:-21353242 Msa1239540:CDS
TCAAGTATGAATGGTTTACT+AGG 0.475295 8_2:-21353104 Msa1239540:three_prime_UTR
CTACCATTTACTCTACTGTA+AGG 0.476278 8_2:-21353188 Msa1239540:CDS
AACCACTGTTCAAGTATGAA+TGG 0.476353 8_2:-21353113 Msa1239540:three_prime_UTR
TCACATTGAGCTTCGCAAAT+GGG 0.477326 8_2:-21354701 Msa1239540:CDS
CACATGTATCGTGCGAGCCT+GGG 0.485764 8_2:-21353382 Msa1239540:CDS
CTCATCTCCATTGACGAGCT+TGG 0.486057 8_2:-21353285 Msa1239540:CDS
ACAGTAGAGTAAATGGTAGA+AGG 0.491124 8_2:+21353192 None:intergenic
ACTTGAAACTCCTGTTTGAA+TGG 0.492773 8_2:-21355040 Msa1239540:five_prime_UTR
ATGGCTGGTGCAACAAAGAA+TGG 0.494922 8_2:+21353348 None:intergenic
AGAAATACCAAGCTCGTCAA+TGG 0.495582 8_2:+21353278 None:intergenic
TCGCAAATGGGCTGATATCA+TGG 0.497396 8_2:-21354689 Msa1239540:CDS
AAACTGGTTGAAGTAATTTG+AGG 0.501499 8_2:+21352830 None:intergenic
CCAAGAGTGTTTGCCGACAA+TGG 0.502597 8_2:+21354658 None:intergenic
CATGGTCATTGCTCCATTGT+CGG 0.504477 8_2:-21354671 Msa1239540:CDS
TTGAAATCTCTGGTTGATGA+TGG 0.505971 8_2:-21354998 Msa1239540:exon
GTATACAAAATCACATCCTT+GGG 0.508904 8_2:+21354772 None:intergenic
CACATAAAAGTGTTCATGGC+TGG 0.518632 8_2:+21353333 None:intergenic
TACAAAGAGGTTAGCTTGTG+GGG 0.520289 8_2:-21353241 Msa1239540:CDS
CCAGAACCTGTGAGTTCAGT+TGG 0.528188 8_2:-21354970 Msa1239540:CDS
ATGAGATGCCGCTCTGTGAA+AGG 0.531219 8_2:+21353303 None:intergenic
ACACGACCAAATATCCAAAA+GGG 0.534142 8_2:+21352912 None:intergenic
GAATGGCTTGCTATAGTCCC+AGG 0.538623 8_2:+21353365 None:intergenic
TGCTGTTGTAGATTGCTGGA+GGG 0.541864 8_2:-21353422 Msa1239540:intron
ACGACGATGAATGGTCTAGT+TGG 0.542274 8_2:-21354747 Msa1239540:CDS
GCTTGTGGGGATTATGGCAA+TGG 0.544428 8_2:-21353228 Msa1239540:CDS
ACAACAACTTTGATCTTAGG+TGG 0.545975 8_2:-21353004 Msa1239540:three_prime_UTR
GGATTATGGCAATGGTGCAA+TGG 0.551856 8_2:-21353220 Msa1239540:CDS
ACAAAACACGACACACGAGA+CGG 0.565541 8_2:-21356289 Msa1239540:five_prime_UTR
AAGACCCAAACCAATACCAC+CGG 0.568681 8_2:-21356393 Msa1239540:five_prime_UTR
AAATAGCATCGACTTATCAA+GGG 0.569320 8_2:+21353069 None:intergenic
TGAACTCACAGGTTCTGGGC+TGG 0.571240 8_2:+21354975 None:intergenic
TCAAAGGCTCAGCAAGGTAA+CGG 0.571549 8_2:-21353153 Msa1239540:CDS
TGGTCTAGTTGGAAGAAACT+AGG 0.573969 8_2:-21354736 Msa1239540:CDS
TCATTCCACCTGTTACAAAG+AGG 0.579111 8_2:-21353254 Msa1239540:CDS
TGAAACTCCTGTTTGAATGG+TGG 0.582594 8_2:-21355037 Msa1239540:five_prime_UTR
GCTAACCTCTTTGTAACAGG+TGG 0.589007 8_2:+21353249 None:intergenic
TTGAGGCCATTCTACAAACA+AGG 0.593358 8_2:+21352847 None:intergenic
ACACACGAGACGGGAAACAA+AGG 0.596022 8_2:-21356279 Msa1239540:five_prime_UTR
TTCTCTCCAACTGAACTCAC+AGG 0.597337 8_2:+21354964 None:intergenic
AACCATTCATACTTGAACAG+TGG 0.599364 8_2:+21353111 None:intergenic
GTCGGCAAACACTCTTGGCA+AGG 0.605734 8_2:-21354653 Msa1239540:intron
TTGCTGTTGTAGATTGCTGG+AGG 0.607972 8_2:-21353423 Msa1239540:intron
TGACCTCAAACAAAAGCGAA+AGG 0.615486 8_2:+21356237 None:intergenic
TGAAAGAACAGCGATTCCCA+AGG 0.623450 8_2:-21354788 Msa1239540:CDS
TATGAGTCAAAGGCTCAGCA+AGG 0.625267 8_2:-21353159 Msa1239540:CDS
TACTAGTGGAAGTGTTGCTG+CGG 0.634094 8_2:-21354893 Msa1239540:CDS
GTTACAAAGAGGTTAGCTTG+TGG 0.645490 8_2:-21353243 Msa1239540:CDS
GTAAAATCCGAGGCTTCCTG+GGG 0.650776 8_2:+21354920 None:intergenic
AGACCCAAACCAATACCACC+GGG 0.663843 8_2:-21356392 Msa1239540:five_prime_UTR
AATAGCATCGACTTATCAAG+GGG 0.669762 8_2:+21353070 None:intergenic
CTAGTAGCGAGTAAAATCCG+AGG 0.704126 8_2:+21354910 None:intergenic
GGAACAATCCTTTCACAGAG+CGG 0.724970 8_2:-21353311 Msa1239540:CDS
TAAAATCCGAGGCTTCCTGG+GGG 0.763745 8_2:+21354921 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATATTTTAATAACCA+AGG - chr8_2:21354056-21354075 Msa1239540:intron 10.0%
!!! TCTTTTTGTAAATTATAAGA+GGG + chr8_2:21352860-21352879 None:intergenic 15.0%
!!! ATCTTTTTGTAAATTATAAG+AGG + chr8_2:21352861-21352880 None:intergenic 15.0%
!!! AGATTTTTTTTTTCTTTTCT+TGG - chr8_2:21352877-21352896 Msa1239540:three_prime_UTR 15.0%
!!! TGATTTTCTGAAATTATTAT+AGG - chr8_2:21353916-21353935 Msa1239540:intron 15.0%
!! TATATTCTATCTACTTATAA+AGG - chr8_2:21354617-21354636 Msa1239540:intron 15.0%
!! TTAGAAATTGTAAAAGAAAA+TGG + chr8_2:21354722-21354741 None:intergenic 15.0%
!!! AATGTTTTTAGAAAGTTAAT+AGG - chr8_2:21354925-21354944 Msa1239540:CDS 15.0%
!!! GGAAAATGTATTTTGATTTT+AGG - chr8_2:21353120-21353139 Msa1239540:three_prime_UTR 20.0%
!!! TTAACAATGCTTGTATATTT+TGG - chr8_2:21353636-21353655 Msa1239540:intron 20.0%
!! AGTTTATATTTCAGTTTAAC+AGG - chr8_2:21353701-21353720 Msa1239540:intron 20.0%
!! TTCAATGTAATCATATTTGT+CGG + chr8_2:21353891-21353910 None:intergenic 20.0%
!!! CAGTATAGAATATACATTTT+AGG - chr8_2:21353994-21354013 Msa1239540:intron 20.0%
!!! ATAGAATATACATTTTAGGT+TGG - chr8_2:21353998-21354017 Msa1239540:intron 20.0%
!!! TAGAATATACATTTTAGGTT+GGG - chr8_2:21353999-21354018 Msa1239540:intron 20.0%
!! TTGAATATAGATGAATCTAA+TGG + chr8_2:21354036-21354055 None:intergenic 20.0%
!! TTCAGTTTAAATTACTAACA+AGG + chr8_2:21354879-21354898 None:intergenic 20.0%
!! TGTTAGTTAGATTTCTATTT+GGG + chr8_2:21355260-21355279 None:intergenic 20.0%
!! TTGTTAGTTAGATTTCTATT+TGG + chr8_2:21355261-21355280 None:intergenic 20.0%
!! TGTTAGGATTATAGAATAAA+GGG + chr8_2:21355664-21355683 None:intergenic 20.0%
!! ATGTTAGGATTATAGAATAA+AGG + chr8_2:21355665-21355684 None:intergenic 20.0%
!! GTAAAAAGACAAATAAAAAC+TGG + chr8_2:21356404-21356423 None:intergenic 20.0%
! TTCTATTACATTACACACTA+TGG + chr8_2:21353349-21353368 None:intergenic 25.0%
!! TTAGAAGTTGGTTTATGATT+TGG - chr8_2:21353763-21353782 Msa1239540:intron 25.0%
! CTTTACATATTACTACTACA+TGG + chr8_2:21353797-21353816 None:intergenic 25.0%
!! TCTGAAATTATTATAGGTGT+CGG - chr8_2:21353922-21353941 Msa1239540:intron 25.0%
!!! ACCAAGGAATCTTTTTTAAA+TGG - chr8_2:21354072-21354091 Msa1239540:intron 25.0%
! TCCATTTAAAAAAGATTCCT+TGG + chr8_2:21354076-21354095 None:intergenic 25.0%
!! ATTTTACGTTACCAATTTGA+AGG - chr8_2:21354137-21354156 Msa1239540:intron 25.0%
! CAAAAAACAAACCTTCAAAT+TGG + chr8_2:21354151-21354170 None:intergenic 25.0%
! GTTGAATTGAAAATGAAAAC+AGG + chr8_2:21354662-21354681 None:intergenic 25.0%
! GCATATATCTTAGTTGTAAT+TGG + chr8_2:21355117-21355136 None:intergenic 25.0%
! ACCTTCTATTACAAAAGAAA+GGG - chr8_2:21355176-21355195 Msa1239540:intron 25.0%
! ATTACAAAAGAAAGGGATAA+TGG - chr8_2:21355183-21355202 Msa1239540:intron 25.0%
! TTACAAAAGAAAGGGATAAT+GGG - chr8_2:21355184-21355203 Msa1239540:intron 25.0%
! TCTATAATCCTAACATATGA+TGG - chr8_2:21355669-21355688 Msa1239540:intron 25.0%
! ATTACAACAACTTTGATCTT+AGG - chr8_2:21356208-21356227 Msa1239540:intron 25.0%
! ATTATAAGAGGGAAGACTTT+TGG + chr8_2:21352849-21352868 None:intergenic 30.0%
!!! TTCCTACACTTTTTCAATGA+TGG - chr8_2:21353087-21353106 Msa1239540:three_prime_UTR 30.0%
! TACCATCATTGAAAAAGTGT+AGG + chr8_2:21353092-21353111 None:intergenic 30.0%
!!! ACTTTTTCAATGATGGTAGA+GGG - chr8_2:21353094-21353113 Msa1239540:three_prime_UTR 30.0%
GGGAAGAACAATGTATTAAT+GGG - chr8_2:21353265-21353284 Msa1239540:CDS 30.0%
AGTGTGTAATGTAATAGAAC+AGG - chr8_2:21353350-21353369 Msa1239540:CDS 30.0%
!!! TAGTCTGTTTTGATTCGATA+AGG + chr8_2:21353402-21353421 None:intergenic 30.0%
CGAGATAAAATCTCTATAAG+AGG + chr8_2:21353673-21353692 None:intergenic 30.0%
! AACTCATGTCATTTAGAAGT+TGG - chr8_2:21353751-21353770 Msa1239540:intron 30.0%
ATTATGTACTGTCCTCTAAA+GGG - chr8_2:21354114-21354133 Msa1239540:intron 30.0%
TAAATATCCACCATTCAAAC+AGG + chr8_2:21354188-21354207 None:intergenic 30.0%
AAGATCTTTGTTGAAATCTC+TGG - chr8_2:21354207-21354226 Msa1239540:intron 30.0%
TTTGTCATTGTTTCACTGAA+TGG - chr8_2:21354360-21354379 Msa1239540:intron 30.0%
TTGTCATTGTTTCACTGAAT+GGG - chr8_2:21354361-21354380 Msa1239540:intron 30.0%
! AAAATGCAAAGACGATTTTG+TGG + chr8_2:21354405-21354424 None:intergenic 30.0%
GTATACAAAATCACATCCTT+GGG + chr8_2:21354446-21354465 None:intergenic 30.0%
! CAAGGATGTGATTTTGTATA+CGG - chr8_2:21354445-21354464 Msa1239540:intron 30.0%
GAAAATGAAAACAGGAAACA+TGG + chr8_2:21354654-21354673 None:intergenic 30.0%
TCTAGAAACAATACAAACAG+TGG + chr8_2:21354786-21354805 None:intergenic 30.0%
CAATTGTGATAGTAAGAAGA+TGG + chr8_2:21354833-21354852 None:intergenic 30.0%
! ATGCCAAAGATACTTTTCTA+AGG - chr8_2:21355133-21355152 Msa1239540:intron 30.0%
! TGCCAAAGATACTTTTCTAA+GGG - chr8_2:21355134-21355153 Msa1239540:intron 30.0%
!! TTCCCTTAGAAAAGTATCTT+TGG + chr8_2:21355139-21355158 None:intergenic 30.0%
GACCTTCTATTACAAAAGAA+AGG - chr8_2:21355175-21355194 Msa1239540:intron 30.0%
! TCCCTTTCTTTTGTAATAGA+AGG + chr8_2:21355180-21355199 None:intergenic 30.0%
!! TTTTGTTGTATTTCCGTTAC+AGG + chr8_2:21355230-21355249 None:intergenic 30.0%
TTACTATGAGTTAGTTAGCT+AGG + chr8_2:21355291-21355310 None:intergenic 30.0%
TACACAACATGTGAAGAATT+CGG + chr8_2:21355525-21355544 None:intergenic 30.0%
GTGTATGTACATCTTATGTA+TGG - chr8_2:21355698-21355717 Msa1239540:intron 30.0%
TCAAGTATGAATGGTTTACT+AGG - chr8_2:21356111-21356130 Msa1239540:intron 30.0%
!! AAATAGCATCGACTTATCAA+GGG + chr8_2:21356149-21356168 None:intergenic 30.0%
!! AAAATAGCATCGACTTATCA+AGG + chr8_2:21356150-21356169 None:intergenic 30.0%
! TTGTTTCCTTGTTTGTAGAA+TGG - chr8_2:21356362-21356381 Msa1239540:five_prime_UTR 30.0%
AAACTGGTTGAAGTAATTTG+AGG + chr8_2:21356388-21356407 None:intergenic 30.0%
!!! CACTTTTTCAATGATGGTAG+AGG - chr8_2:21353093-21353112 Msa1239540:three_prime_UTR 35.0%
CACGCAAATCATCAAAAAGA+GGG + chr8_2:21353202-21353221 None:intergenic 35.0%
ACACGCAAATCATCAAAAAG+AGG + chr8_2:21353203-21353222 None:intergenic 35.0%
TCGTGTGATTAATCGTTAGT+CGG - chr8_2:21353242-21353261 Msa1239540:CDS 35.0%
GGGGAAGAACAATGTATTAA+TGG - chr8_2:21353264-21353283 Msa1239540:CDS 35.0%
!! AACAACAACCAAGCCTTATT+CGG - chr8_2:21353299-21353318 Msa1239540:CDS 35.0%
AGTTTAACAGGACAAAGAGA+TGG - chr8_2:21353713-21353732 Msa1239540:intron 35.0%
CTTTCAGAACATATGAAGCA+TGG - chr8_2:21353836-21353855 Msa1239540:intron 35.0%
TCTATACTGTCTATGAAGCA+CGG + chr8_2:21353983-21354002 None:intergenic 35.0%
CATTATGTACTGTCCTCTAA+AGG - chr8_2:21354113-21354132 Msa1239540:intron 35.0%
GTAACGTAAAATCCCTTTAG+AGG + chr8_2:21354129-21354148 None:intergenic 35.0%
ACTTGAAACTCCTGTTTGAA+TGG - chr8_2:21354175-21354194 Msa1239540:intron 35.0%
!! TTGAAATCTCTGGTTGATGA+TGG - chr8_2:21354217-21354236 Msa1239540:intron 35.0%
CGTATACAAAATCACATCCT+TGG + chr8_2:21354447-21354466 None:intergenic 35.0%
ATAGTAAGAAGATGGAAGAC+TGG + chr8_2:21354825-21354844 None:intergenic 35.0%
!! GCTAACATCATATAACGGAT+AGG + chr8_2:21355005-21355024 None:intergenic 35.0%
GCGTTGCTAACATCATATAA+CGG + chr8_2:21355010-21355029 None:intergenic 35.0%
!! GATACTTTTCTAAGGGAAGA+AGG - chr8_2:21355141-21355160 Msa1239540:intron 35.0%
!! AAAGAAAGGGATAATGGGTA+AGG - chr8_2:21355189-21355208 Msa1239540:intron 35.0%
AGTAACATAATTCGTGCACT+AGG - chr8_2:21355306-21355325 Msa1239540:intron 35.0%
AATCAGCGTTATCCTTTCTT+TGG + chr8_2:21355443-21355462 None:intergenic 35.0%
AAAGAAAGGATAACGCTGAT+TGG - chr8_2:21355442-21355461 Msa1239540:intron 35.0%
! ACGCTGATTGGTGAAAAAAA+CGG - chr8_2:21355454-21355473 Msa1239540:intron 35.0%
ACATAATTCGCAATTCGCAT+TGG + chr8_2:21355568-21355587 None:intergenic 35.0%
ACAACTGACCATCATATGTT+AGG + chr8_2:21355680-21355699 None:intergenic 35.0%
!! GTTCCACATAAAAGTGTTCA+TGG + chr8_2:21355889-21355908 None:intergenic 35.0%
!! TTACAAAGAGGTTAGCTTGT+GGG - chr8_2:21355973-21355992 Msa1239540:intron 35.0%
ACAGTAGAGTAAATGGTAGA+AGG + chr8_2:21356026-21356045 None:intergenic 35.0%
CTACCATTTACTCTACTGTA+AGG - chr8_2:21356027-21356046 Msa1239540:intron 35.0%
! AAGGCTCTTTTATGAGTCAA+AGG - chr8_2:21356046-21356065 Msa1239540:intron 35.0%
AACCACTGTTCAAGTATGAA+TGG - chr8_2:21356102-21356121 Msa1239540:intron 35.0%
AACCATTCATACTTGAACAG+TGG + chr8_2:21356107-21356126 None:intergenic 35.0%
!! AATAGCATCGACTTATCAAG+GGG + chr8_2:21356148-21356167 None:intergenic 35.0%
ACAACAACTTTGATCTTAGG+TGG - chr8_2:21356211-21356230 Msa1239540:intron 35.0%
GTGGTGTGTAATAGTGTAAA+TGG - chr8_2:21356230-21356249 Msa1239540:intron 35.0%
! ATTCTCCTTTTATGTCTCAG+AGG - chr8_2:21356262-21356281 Msa1239540:five_prime_UTR 35.0%
! TTCTCCTTTTATGTCTCAGA+GGG - chr8_2:21356263-21356282 Msa1239540:five_prime_UTR 35.0%
! TACACACCCTTTTGGATATT+TGG - chr8_2:21356297-21356316 Msa1239540:five_prime_UTR 35.0%
ACACGACCAAATATCCAAAA+GGG + chr8_2:21356306-21356325 None:intergenic 35.0%
TACACGACCAAATATCCAAA+AGG + chr8_2:21356307-21356326 None:intergenic 35.0%
!! ACTCCTTTCGCTTTTGTTTG+AGG - chr8_2:21352975-21352994 Msa1239540:three_prime_UTR 40.0%
TGACCTCAAACAAAAGCGAA+AGG + chr8_2:21352981-21353000 None:intergenic 40.0%
TTCAATGATGGTAGAGGGTT+AGG - chr8_2:21353099-21353118 Msa1239540:three_prime_UTR 40.0%
CGTGTGATTAATCGTTAGTC+GGG - chr8_2:21353243-21353262 Msa1239540:CDS 40.0%
GTGTGATTAATCGTTAGTCG+GGG - chr8_2:21353244-21353263 Msa1239540:CDS 40.0%
TGTGATTAATCGTTAGTCGG+GGG - chr8_2:21353245-21353264 Msa1239540:CDS 40.0%
ATTCCTGTTTACACTGCAGT+AGG - chr8_2:21353545-21353564 Msa1239540:intron 40.0%
TTTCCTACTGCAGTGTAAAC+AGG + chr8_2:21353551-21353570 None:intergenic 40.0%
TAACAGGACAAAGAGATGGT+AGG - chr8_2:21353717-21353736 Msa1239540:intron 40.0%
TGAAACTCCTGTTTGAATGG+TGG - chr8_2:21354178-21354197 Msa1239540:intron 40.0%
TGGTCTAGTTGGAAGAAACT+AGG - chr8_2:21354479-21354498 Msa1239540:intron 40.0%
TTCACATTGAGCTTCGCAAA+TGG - chr8_2:21354513-21354532 Msa1239540:intron 40.0%
TCACATTGAGCTTCGCAAAT+GGG - chr8_2:21354514-21354533 Msa1239540:intron 40.0%
ACAGGAAACATGGAAGCAAA+CGG + chr8_2:21354644-21354663 None:intergenic 40.0%
CTTGTCGGAACTAAAGCAAT+TGG + chr8_2:21354964-21354983 None:intergenic 40.0%
ACATCGTCACACAAACTTGT+CGG + chr8_2:21354979-21354998 None:intergenic 40.0%
CCTTAGCAACTAACCTGTAA+CGG - chr8_2:21355214-21355233 Msa1239540:intron 40.0%
GAGTTAGTTAGCTAGGTTCA+AGG + chr8_2:21355284-21355303 None:intergenic 40.0%
TATGTAGCAGTACCCGAATT+CGG + chr8_2:21355368-21355387 None:intergenic 40.0%
TTGAGATGCATCAATTCACG+TGG - chr8_2:21355388-21355407 Msa1239540:intron 40.0%
!! TGTTTGCTGTTGTAGATTGC+TGG - chr8_2:21355789-21355808 Msa1239540:intron 40.0%
!! CACATAAAAGTGTTCATGGC+TGG + chr8_2:21355885-21355904 None:intergenic 40.0%
! CAGCCATGAACACTTTTATG+TGG - chr8_2:21355883-21355902 Msa1239540:intron 40.0%
AGAAATACCAAGCTCGTCAA+TGG + chr8_2:21355940-21355959 None:intergenic 40.0%
TCATTCCACCTGTTACAAAG+AGG - chr8_2:21355961-21355980 Msa1239540:intron 40.0%
CAAGCTAACCTCTTTGTAAC+AGG + chr8_2:21355972-21355991 None:intergenic 40.0%
!! GTTACAAAGAGGTTAGCTTG+TGG - chr8_2:21355972-21355991 Msa1239540:intron 40.0%
!! TACAAAGAGGTTAGCTTGTG+GGG - chr8_2:21355974-21355993 Msa1239540:intron 40.0%
GAGCCTTACAGTAGAGTAAA+TGG + chr8_2:21356033-21356052 None:intergenic 40.0%
! GGTTTGTATACACACCCTTT+TGG - chr8_2:21356289-21356308 Msa1239540:five_prime_UTR 40.0%
TTGAGGCCATTCTACAAACA+AGG + chr8_2:21356371-21356390 None:intergenic 40.0%
AAGACCCAAACCAATACCAC+CGG - chr8_2:21352822-21352841 Msa1239540:three_prime_UTR 45.0%
ACAAAACACGACACACGAGA+CGG - chr8_2:21352926-21352945 Msa1239540:three_prime_UTR 45.0%
!! CAACCAAGCCTTATTCGGAT+CGG - chr8_2:21353304-21353323 Msa1239540:CDS 45.0%
CTATGTAGCCGATCCGAATA+AGG + chr8_2:21353315-21353334 None:intergenic 45.0%
!! AGAACAGGTAAAAAGCGTCG+AGG - chr8_2:21353365-21353384 Msa1239540:CDS 45.0%
GCACAAATCTCACTGTCGAT+AGG + chr8_2:21353434-21353453 None:intergenic 45.0%
! AAGAGAGTGTGAGTCCTACT+AGG - chr8_2:21353492-21353511 Msa1239540:intron 45.0%
! AGAGAGTGTGAGTCCTACTA+GGG - chr8_2:21353493-21353512 Msa1239540:intron 45.0%
AAAGAGATGGTAGGTGGCTT+TGG - chr8_2:21353726-21353745 Msa1239540:intron 45.0%
!! CATATTTGTCGGTGTCGTGT+CGG + chr8_2:21353880-21353899 None:intergenic 45.0%
CAACTGAACTCACAGGTTCT+GGG + chr8_2:21354247-21354266 None:intergenic 45.0%
TTCTCTCCAACTGAACTCAC+AGG + chr8_2:21354254-21354273 None:intergenic 45.0%
AGTAAAATCCGAGGCTTCCT+GGG + chr8_2:21354299-21354318 None:intergenic 45.0%
CTAGTAGCGAGTAAAATCCG+AGG + chr8_2:21354308-21354327 None:intergenic 45.0%
! CGGATTTTACTCGCTACTAG+TGG - chr8_2:21354308-21354327 Msa1239540:intron 45.0%
!! TACTAGTGGAAGTGTTGCTG+CGG - chr8_2:21354322-21354341 Msa1239540:intron 45.0%
TGAAAGAACAGCGATTCCCA+AGG - chr8_2:21354427-21354446 Msa1239540:intron 45.0%
TGTATACGGACGACGATGAA+TGG - chr8_2:21354459-21354478 Msa1239540:intron 45.0%
ACGACGATGAATGGTCTAGT+TGG - chr8_2:21354468-21354487 Msa1239540:intron 45.0%
!! TCGCAAATGGGCTGATATCA+TGG - chr8_2:21354526-21354545 Msa1239540:intron 45.0%
CATGGTCATTGCTCCATTGT+CGG - chr8_2:21354544-21354563 Msa1239540:intron 45.0%
CAGGAAACATGGAAGCAAAC+GGG + chr8_2:21354643-21354662 None:intergenic 45.0%
! AAGAAGATGGAAGACTGGCT+AGG + chr8_2:21354820-21354839 None:intergenic 45.0%
GATGTTAGCAACGCAAGAGT+TGG - chr8_2:21355016-21355035 Msa1239540:five_prime_UTR 45.0%
! CCGTTACAGGTTAGTTGCTA+AGG + chr8_2:21355217-21355236 None:intergenic 45.0%
!! CAATTCGCGAGTACCGAATT+CGG - chr8_2:21355352-21355371 Msa1239540:intron 45.0%
!! AATTCGCGAGTACCGAATTC+GGG - chr8_2:21355353-21355372 Msa1239540:intron 45.0%
TTTGCACAAACTGCCATACC+AGG - chr8_2:21355413-21355432 Msa1239540:intron 45.0%
TTTCTTTGGTACGCCTGGTA+TGG + chr8_2:21355429-21355448 None:intergenic 45.0%
ATACCAGGCGTACCAAAGAA+AGG - chr8_2:21355428-21355447 Msa1239540:intron 45.0%
TATCCTTTCTTTGGTACGCC+TGG + chr8_2:21355434-21355453 None:intergenic 45.0%
! TCGCAATTCGCATTGGTACA+CGG + chr8_2:21355561-21355580 None:intergenic 45.0%
! TTGCTGTTGTAGATTGCTGG+AGG - chr8_2:21355792-21355811 Msa1239540:intron 45.0%
TGCTGTTGTAGATTGCTGGA+GGG - chr8_2:21355793-21355812 Msa1239540:intron 45.0%
ATGGCTGGTGCAACAAAGAA+TGG + chr8_2:21355870-21355889 None:intergenic 45.0%
GGAACAATCCTTTCACAGAG+CGG - chr8_2:21355904-21355923 Msa1239540:intron 45.0%
GCTAACCTCTTTGTAACAGG+TGG + chr8_2:21355969-21355988 None:intergenic 45.0%
!! AGGTTAGCTTGTGGGGATTA+TGG - chr8_2:21355981-21356000 Msa1239540:intron 45.0%
GGATTATGGCAATGGTGCAA+TGG - chr8_2:21355995-21356014 Msa1239540:intron 45.0%
TATGAGTCAAAGGCTCAGCA+AGG - chr8_2:21356056-21356075 Msa1239540:intron 45.0%
TCAAAGGCTCAGCAAGGTAA+CGG - chr8_2:21356062-21356081 Msa1239540:intron 45.0%
!! CTCACCCTCTGAGACATAAA+AGG + chr8_2:21356270-21356289 None:intergenic 45.0%
! CTTTTATGTCTCAGAGGGTG+AGG - chr8_2:21356268-21356287 Msa1239540:five_prime_UTR 45.0%
AGACCCAAACCAATACCACC+GGG - chr8_2:21352823-21352842 Msa1239540:three_prime_UTR 50.0%
! AAGAGGGAAGACTTTTGGCC+CGG + chr8_2:21352844-21352863 None:intergenic 50.0%
CAAAACACGACACACGAGAC+GGG - chr8_2:21352927-21352946 Msa1239540:three_prime_UTR 50.0%
ACACACGAGACGGGAAACAA+AGG - chr8_2:21352936-21352955 Msa1239540:three_prime_UTR 50.0%
TAGCCGATCCGAATAAGGCT+TGG + chr8_2:21353310-21353329 None:intergenic 50.0%
! GAGAGTGTGAGTCCTACTAG+GGG - chr8_2:21353494-21353513 Msa1239540:intron 50.0%
! CACTTGAAGCATCCCCTAGT+AGG + chr8_2:21353509-21353528 None:intergenic 50.0%
CTACTAGGGGATGCTTCAAG+TGG - chr8_2:21353507-21353526 Msa1239540:intron 50.0%
CAGGACAAAGAGATGGTAGG+TGG - chr8_2:21353720-21353739 Msa1239540:intron 50.0%
ACGGATACAGACACGTACAC+CGG + chr8_2:21353964-21353983 None:intergenic 50.0%
CCAACTGAACTCACAGGTTC+TGG + chr8_2:21354248-21354267 None:intergenic 50.0%
CCAGAACCTGTGAGTTCAGT+TGG - chr8_2:21354245-21354264 Msa1239540:intron 50.0%
TAAAATCCGAGGCTTCCTGG+GGG + chr8_2:21354297-21354316 None:intergenic 50.0%
GTAAAATCCGAGGCTTCCTG+GGG + chr8_2:21354298-21354317 None:intergenic 50.0%
GAGTAAAATCCGAGGCTTCC+TGG + chr8_2:21354300-21354319 None:intergenic 50.0%
!! CCAAGAGTGTTTGCCGACAA+TGG + chr8_2:21354560-21354579 None:intergenic 50.0%
CCATTGTCGGCAAACACTCT+TGG - chr8_2:21354557-21354576 Msa1239540:intron 50.0%
! CGAATTGGTTCACGCCTTCA+CGG + chr8_2:21355339-21355358 None:intergenic 50.0%
CGAATTCGGTACTCGCGAAT+TGG + chr8_2:21355354-21355373 None:intergenic 50.0%
! TTCGCATTGGTACACGGTAC+AGG + chr8_2:21355555-21355574 None:intergenic 50.0%
!! GAATGGCTTGCTATAGTCCC+AGG + chr8_2:21355853-21355872 None:intergenic 50.0%
ATGAGATGCCGCTCTGTGAA+AGG + chr8_2:21355915-21355934 None:intergenic 50.0%
CTCATCTCCATTGACGAGCT+TGG - chr8_2:21355930-21355949 Msa1239540:intron 50.0%
GCTTGTGGGGATTATGGCAA+TGG - chr8_2:21355987-21356006 Msa1239540:intron 50.0%
!! TGGCCCGGTGGTATTGGTTT+GGG + chr8_2:21352829-21352848 None:intergenic 55.0%
!! TTGGCCCGGTGGTATTGGTT+TGG + chr8_2:21352830-21352849 None:intergenic 55.0%
! GACTTTTGGCCCGGTGGTAT+TGG + chr8_2:21352835-21352854 None:intergenic 55.0%
!! TGAACTCACAGGTTCTGGGC+TGG + chr8_2:21354243-21354262 None:intergenic 55.0%
GTCGGCAAACACTCTTGGCA+AGG - chr8_2:21354562-21354581 Msa1239540:intron 55.0%
GAGAGCAAGCTCTTCTGCAC+TGG - chr8_2:21355083-21355102 Msa1239540:intron 55.0%
CACTAGGCAGAGAACCGTGA+AGG - chr8_2:21355322-21355341 Msa1239540:intron 55.0%
TGGTACACGGTACAGGTTCG+CGG + chr8_2:21355548-21355567 None:intergenic 55.0%
TCACATGTATCGTGCGAGCC+TGG - chr8_2:21355832-21355851 Msa1239540:intron 55.0%
CACATGTATCGTGCGAGCCT+GGG - chr8_2:21355833-21355852 Msa1239540:intron 55.0%
! AGGGAAGACTTTTGGCCCGG+TGG + chr8_2:21352841-21352860 None:intergenic 60.0%
GTGTCGGAGTTGGACACGCT+CGG + chr8_2:21353864-21353883 None:intergenic 60.0%
!! TGTCGGTGTCGTGTCGGAGT+TGG + chr8_2:21353874-21353893 None:intergenic 60.0%
CACGTACACCGGACACGACA+CGG + chr8_2:21353953-21353972 None:intergenic 60.0%
CGGCATGTCCGTGTCGTGTC+CGG - chr8_2:21353942-21353961 Msa1239540:intron 65.0%
CAGTGAATGCTGCTGCCCCC+AGG - chr8_2:21354279-21354298 Msa1239540:intron 65.0%
CTGCTGCCCCCAGGAAGCCT+CGG - chr8_2:21354288-21354307 Msa1239540:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 21352806 21356431 21352806 ID=Msa1239540;Name=Msa1239540
chr8_2 mRNA 21352806 21356431 21352806 ID=Msa1239540-mRNA-1;Parent=Msa1239540;Name=Msa1239540-mRNA-1;_AED=0.01;_eAED=0.01;_QI=265|1|1|1|0.5|0.33|3|337|213
chr8_2 exon 21352806 21353433 21352806 ID=Msa1239540-mRNA-1:exon:7640;Parent=Msa1239540-mRNA-1
chr8_2 exon 21354654 21355078 21354654 ID=Msa1239540-mRNA-1:exon:7639;Parent=Msa1239540-mRNA-1
chr8_2 exon 21356241 21356431 21356241 ID=Msa1239540-mRNA-1:exon:7638;Parent=Msa1239540-mRNA-1
chr8_2 five_prime_UTR 21356241 21356431 21356241 ID=Msa1239540-mRNA-1:five_prime_utr;Parent=Msa1239540-mRNA-1
chr8_2 five_prime_UTR 21355005 21355078 21355005 ID=Msa1239540-mRNA-1:five_prime_utr;Parent=Msa1239540-mRNA-1
chr8_2 CDS 21354654 21355004 21354654 ID=Msa1239540-mRNA-1:cds;Parent=Msa1239540-mRNA-1
chr8_2 CDS 21353143 21353433 21353143 ID=Msa1239540-mRNA-1:cds;Parent=Msa1239540-mRNA-1
chr8_2 three_prime_UTR 21352806 21353142 21352806 ID=Msa1239540-mRNA-1:three_prime_utr;Parent=Msa1239540-mRNA-1
Gene Sequence

>Msa1239540

ATGATGGCCAGCCCAGAACCTGTGAGTTCAGTTGGAGAGAATATGTCAGTGAATGCTGCTGCCCCCAGGAAGCCTCGGATTTTACTCGCTACTAGTGGAAGTGTTGCTGCGGTTAAATTTGCAAATCTTTGTCATTGTTTCACTGAATGGGCAGAAGTAAGAGCTGTTGCCACAAAATCGTCTTTGCATTTTATTGAAAGAACAGCGATTCCCAAGGATGTGATTTTGTATACGGACGACGATGAATGGTCTAGTTGGAAGAAACTAGGCGATAGCGTGCTTCACATTGAGCTTCGCAAATGGGCTGATATCATGGTCATTGCTCCATTGTCGGCAAACACTCTTGGCAAGATTGCTGGAGGGTTGTGTGACAATCTACTCACATGTATCGTGCGAGCCTGGGACTATAGCAAGCCATTCTTTGTTGCACCAGCCATGAACACTTTTATGTGGAACAATCCTTTCACAGAGCGGCATCTCATCTCCATTGACGAGCTTGGTATTTCTCTCATTCCACCTGTTACAAAGAGGTTAGCTTGTGGGGATTATGGCAATGGTGCAATGGCTGAACCTTCTACCATTTACTCTACTGTAAGGCTCTTTTATGAGTCAAAGGCTCAGCAAGGTAACGGCGATGTTTAG
Protein sequence

>Msa1239540

MMASPEPVSSVGENMSVNAAAPRKPRILLATSGSVAAVKFANLCHCFTEWAEVRAVATKSSLHFIERTAIPKDVILYTDDDEWSSWKKLGDSVLHIELRKWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYSKPFFVAPAMNTFMWNNPFTERHLISIDELGISLIPPVTKRLACGDYGNGAMAEPSTIYSTVRLFYESKAQQGNGDV*