AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1320130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1320130 MtrunA17_Chr8g0351241 99.531 213 1 0 1 213 1 213 1.20e-160 442
Msa1320130 MtrunA17_Chr7g0246291 57.653 196 80 2 9 203 5 198 8.80e-81 239
Msa1320130 MtrunA17_Chr7g0246611 57.653 196 80 2 9 203 5 198 8.80e-81 239
Msa1320130 MtrunA17_Chr7g0246621 56.938 209 77 3 1 205 1 200 4.32e-78 233
Msa1320130 MtrunA17_Chr7g0246261 58.173 208 74 4 1 204 1 199 1.37e-77 231
Msa1320130 MtrunA17_Chr7g0246281 57.812 192 76 2 15 205 1 188 3.67e-73 220
Msa1320130 MtrunA17_Chr7g0246601 59.231 130 47 2 76 205 5 128 7.27e-47 151
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1320130 AT3G18030.1 78.894 199 41 1 13 211 9 206 5.86e-124 349
Msa1320130 AT1G48605.1 76.923 195 44 1 14 208 2 195 6.40e-118 333

Find 83 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTACAACAACTTTGATCTT+AGG 0.238461 8_4:-24400222 Msa1320130:three_prime_UTR
CCAACTGAACTCACAGGTTC+TGG 0.249432 8_4:+24402185 None:intergenic
AGGTTAGCTTGTGGGGATTA+TGG 0.262541 8_4:-24400449 Msa1320130:CDS
GAGCCTTACAGTAGAGTAAA+TGG 0.285370 8_4:+24400400 None:intergenic
TTGGCCTGGTGGTATTGGTT+TGG 0.293364 8_4:+24403631 None:intergenic
ATTATAAGAGGGAAGACTTT+TGG 0.297280 8_4:+24403612 None:intergenic
TGGCCTGGTGGTATTGGTTT+GGG 0.298566 8_4:+24403632 None:intergenic
GAGTAAAATCCGAGGCTTCC+TGG 0.300240 8_4:+24402133 None:intergenic
GTTCCACATAAAAGTGTTCA+TGG 0.333551 8_4:+24400544 None:intergenic
CTCACCCTCTGAGACATAAA+AGG 0.344105 8_4:+24400163 None:intergenic
GGTTTGTATACACACCCTTT+TGG 0.357670 8_4:-24400141 Msa1320130:three_prime_UTR
AAGATCTTTGTTGAAATCTC+TGG 0.366216 8_4:-24402223 Msa1320130:five_prime_UTR
TTTGTCATTGTTTCACTGAA+TGG 0.370610 8_4:-24402070 Msa1320130:CDS
TGTTTGCTGTTGTAGATTGC+TGG 0.375544 8_4:-24400641 Msa1320130:intron
TCACATGTATCGTGCGAGCC+TGG 0.385273 8_4:-24400598 Msa1320130:CDS
CAAGCTAACCTCTTTGTAAC+AGG 0.386122 8_4:+24400461 None:intergenic
GTGGTGTGTAATAGTGTAAA+TGG 0.407317 8_4:-24400200 Msa1320130:three_prime_UTR
AAAATAGCATCGACTTATCA+AGG 0.416965 8_4:+24400283 None:intergenic
AGTAAAATCCGAGGCTTCCT+GGG 0.419242 8_4:+24402134 None:intergenic
TTGTCATTGTTTCACTGAAT+GGG 0.424119 8_4:-24402069 Msa1320130:CDS
TTCACATTGAGCTTCGCAAA+TGG 0.429618 8_4:-24401917 Msa1320130:CDS
TACACGACCAAATATCCAAA+AGG 0.439002 8_4:+24400126 None:intergenic
TAAATATCCACCATTCAAAC+AGG 0.444612 8_4:+24402245 None:intergenic
CGTATACAAAATCACATCCT+TGG 0.455438 8_4:+24401986 None:intergenic
TGTATACGGACGACGATGAA+TGG 0.456117 8_4:-24401971 Msa1320130:CDS
CAACTGAACTCACAGGTTCT+GGG 0.458227 8_4:+24402186 None:intergenic
TTGTTTCCTTGTTTGTAGAA+TGG 0.460477 8_4:-24400068 Msa1320130:three_prime_UTR
CCATTGTCGGCAAACACTCT+TGG 0.468148 8_4:-24401873 Msa1320130:CDS
CAAAACACGACACACGAGAC+GGG 0.469256 8_4:-24403527 Msa1320130:five_prime_UTR
TTACAAAGAGGTTAGCTTGT+GGG 0.472912 8_4:-24400457 Msa1320130:CDS
TCAAGTATGAATGGTTTACT+AGG 0.475295 8_4:-24400319 Msa1320130:three_prime_UTR
CTACCATTTACTCTACTGTA+AGG 0.476278 8_4:-24400403 Msa1320130:CDS
AACCACTGTTCAAGTATGAA+TGG 0.476353 8_4:-24400328 Msa1320130:three_prime_UTR
TCACATTGAGCTTCGCAAAT+GGG 0.477326 8_4:-24401916 Msa1320130:CDS
CACATGTATCGTGCGAGCCT+GGG 0.485764 8_4:-24400597 Msa1320130:CDS
CTCATCTCCATTGACGAGCT+TGG 0.486057 8_4:-24400500 Msa1320130:CDS
ACAGTAGAGTAAATGGTAGA+AGG 0.491124 8_4:+24400407 None:intergenic
ACTTGAAACTCCTGTTTGAA+TGG 0.492773 8_4:-24402255 Msa1320130:five_prime_UTR
ATGGCTGGTGCAACAAAGAA+TGG 0.494922 8_4:+24400563 None:intergenic
AGAAATACCAAGCTCGTCAA+TGG 0.495582 8_4:+24400493 None:intergenic
TCGCAAATGGGCTGATATCA+TGG 0.497396 8_4:-24401904 Msa1320130:CDS
AAACTGGTTGAAGTAATTTG+AGG 0.501499 8_4:+24400045 None:intergenic
CCAAGAGTGTTTGCCGACAA+TGG 0.502597 8_4:+24401873 None:intergenic
CATGGTCATTGCTCCATTGT+CGG 0.504477 8_4:-24401886 Msa1320130:CDS
TTGAAATCTCTGGTTGATGA+TGG 0.505971 8_4:-24402213 Msa1320130:exon
GTATACAAAATCACATCCTT+GGG 0.508904 8_4:+24401987 None:intergenic
CACATAAAAGTGTTCATGGC+TGG 0.518632 8_4:+24400548 None:intergenic
TACAAAGAGGTTAGCTTGTG+GGG 0.520289 8_4:-24400456 Msa1320130:CDS
GACACACGAGACGGGAAACA+AGG 0.528142 8_4:-24403519 Msa1320130:five_prime_UTR
CCAGAACCTGTGAGTTCAGT+TGG 0.528188 8_4:-24402185 Msa1320130:CDS
ATGAGATGCCGCTCTGTGAA+AGG 0.531219 8_4:+24400518 None:intergenic
ACACGACCAAATATCCAAAA+GGG 0.534142 8_4:+24400127 None:intergenic
GAATGGCTTGCTATAGTCCC+AGG 0.538623 8_4:+24400580 None:intergenic
TGCTGTTGTAGATTGCTGGA+GGG 0.541864 8_4:-24400637 Msa1320130:intron
ACGACGATGAATGGTCTAGT+TGG 0.542274 8_4:-24401962 Msa1320130:CDS
GCTTGTGGGGATTATGGCAA+TGG 0.544428 8_4:-24400443 Msa1320130:CDS
ACAACAACTTTGATCTTAGG+TGG 0.545975 8_4:-24400219 Msa1320130:three_prime_UTR
GGATTATGGCAATGGTGCAA+TGG 0.551856 8_4:-24400435 Msa1320130:CDS
ACAAAACACGACACACGAGA+CGG 0.565541 8_4:-24403528 Msa1320130:five_prime_UTR
AAATAGCATCGACTTATCAA+GGG 0.569320 8_4:+24400284 None:intergenic
TGAACTCACAGGTTCTGGGC+TGG 0.571240 8_4:+24402190 None:intergenic
TCAAAGGCTCAGCAAGGTAA+CGG 0.571549 8_4:-24400368 Msa1320130:CDS
TGGTCTAGTTGGAAGAAACT+AGG 0.573969 8_4:-24401951 Msa1320130:CDS
TCATTCCACCTGTTACAAAG+AGG 0.579111 8_4:-24400469 Msa1320130:CDS
TGAAACTCCTGTTTGAATGG+TGG 0.582594 8_4:-24402252 Msa1320130:five_prime_UTR
GCTAACCTCTTTGTAACAGG+TGG 0.589007 8_4:+24400464 None:intergenic
TTGAGGCCATTCTACAAACA+AGG 0.593358 8_4:+24400062 None:intergenic
TTCTCTCCAACTGAACTCAC+AGG 0.597337 8_4:+24402179 None:intergenic
AACCATTCATACTTGAACAG+TGG 0.599364 8_4:+24400326 None:intergenic
GTCGGCAAACACTCTTGGCA+AGG 0.605734 8_4:-24401868 Msa1320130:intron
TTGCTGTTGTAGATTGCTGG+AGG 0.607972 8_4:-24400638 Msa1320130:intron
TGAAAGAACAGCGATTCCCA+AGG 0.623450 8_4:-24402003 Msa1320130:CDS
TATGAGTCAAAGGCTCAGCA+AGG 0.625267 8_4:-24400374 Msa1320130:CDS
TACTAGTGGAAGTGTTGCTG+CGG 0.634094 8_4:-24402108 Msa1320130:CDS
TGACCTCAAACAAAAGCGAA+AGG 0.641701 8_4:+24403476 None:intergenic
GTTACAAAGAGGTTAGCTTG+TGG 0.645490 8_4:-24400458 Msa1320130:CDS
GTAAAATCCGAGGCTTCCTG+GGG 0.650776 8_4:+24402135 None:intergenic
AGACCCAAACCAATACCACC+AGG 0.652445 8_4:-24403635 Msa1320130:five_prime_UTR
ACACACGAGACGGGAAACAA+GGG 0.667567 8_4:-24403518 Msa1320130:five_prime_UTR
AATAGCATCGACTTATCAAG+GGG 0.669762 8_4:+24400285 None:intergenic
CTAGTAGCGAGTAAAATCCG+AGG 0.704126 8_4:+24402125 None:intergenic
GGAACAATCCTTTCACAGAG+CGG 0.724970 8_4:-24400526 Msa1320130:CDS
TAAAATCCGAGGCTTCCTGG+GGG 0.763745 8_4:+24402136 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTCTTTTCT+TGG - chr8_4:24400097-24400116 Msa1320130:three_prime_UTR 10.0%
!!! AATTTATATTTTAATAACCA+AGG - chr8_4:24401300-24401319 Msa1320130:intron 10.0%
!!! TCTTTTTGTAAATTATAAGA+GGG + chr8_4:24400076-24400095 None:intergenic 15.0%
!!! ATCTTTTTGTAAATTATAAG+AGG + chr8_4:24400077-24400096 None:intergenic 15.0%
!!! TGATTTTCTGAAATTATTAT+AGG - chr8_4:24401160-24401179 Msa1320130:intron 15.0%
!! TATATTCTATCTACTTATAA+AGG - chr8_4:24401861-24401880 Msa1320130:intron 15.0%
!! TTAGAAATTGTAAAAGAAAA+TGG + chr8_4:24401966-24401985 None:intergenic 15.0%
!!! AATGTTTTTAGAAAGTTAAT+AGG - chr8_4:24402169-24402188 Msa1320130:CDS 15.0%
!!! GGAAAATGTATTTTGATTTT+AGG - chr8_4:24400340-24400359 Msa1320130:exon 20.0%
!!! TTAACAATGCTTGTATATTT+TGG - chr8_4:24400856-24400875 Msa1320130:intron 20.0%
!! AGTTTATATTTCAGTTTAAC+AGG - chr8_4:24400921-24400940 Msa1320130:intron 20.0%
!! TTCAATGTAATCATATTTGT+CGG + chr8_4:24401135-24401154 None:intergenic 20.0%
!!! CAGTATAGAATATACATTTT+AGG - chr8_4:24401238-24401257 Msa1320130:intron 20.0%
!!! ATAGAATATACATTTTAGGT+TGG - chr8_4:24401242-24401261 Msa1320130:intron 20.0%
!!! TAGAATATACATTTTAGGTT+GGG - chr8_4:24401243-24401262 Msa1320130:intron 20.0%
!! TTGAATATAGATGAATCTAA+TGG + chr8_4:24401280-24401299 None:intergenic 20.0%
!! TTCAGTTTAAATTACTAACA+AGG + chr8_4:24402123-24402142 None:intergenic 20.0%
!! TGTTAGTTAGATTTCTATTT+GGG + chr8_4:24402504-24402523 None:intergenic 20.0%
!! TTGTTAGTTAGATTTCTATT+TGG + chr8_4:24402505-24402524 None:intergenic 20.0%
!! TGTTAGGATTATAGAATAAA+GGG + chr8_4:24402908-24402927 None:intergenic 20.0%
!! ATGTTAGGATTATAGAATAA+AGG + chr8_4:24402909-24402928 None:intergenic 20.0%
!! GTAAAAAGACAAATAAAAAC+TGG + chr8_4:24403648-24403667 None:intergenic 20.0%
! TTCTATTACATTACACACTA+TGG + chr8_4:24400569-24400588 None:intergenic 25.0%
!! TTAGAAGTTGGTTTATGATT+TGG - chr8_4:24400983-24401002 Msa1320130:intron 25.0%
! CTTTACATATTACTACTACA+TGG + chr8_4:24401017-24401036 None:intergenic 25.0%
!! TCTGAAATTATTATAGGTGT+CGG - chr8_4:24401166-24401185 Msa1320130:intron 25.0%
!!! ACCAAGGAATCTTTTTTAAA+TGG - chr8_4:24401316-24401335 Msa1320130:intron 25.0%
! TCCATTTAAAAAAGATTCCT+TGG + chr8_4:24401320-24401339 None:intergenic 25.0%
!! ATTTTACGTTACCAATTTGA+AGG - chr8_4:24401381-24401400 Msa1320130:intron 25.0%
! CAAAAAACAAACCTTCAAAT+TGG + chr8_4:24401395-24401414 None:intergenic 25.0%
! GTTGAATTGAAAATGAAAAC+AGG + chr8_4:24401906-24401925 None:intergenic 25.0%
! GCATATATCTTAGTTGTAAT+TGG + chr8_4:24402361-24402380 None:intergenic 25.0%
! TCTATAATCCTAACATATGA+TGG - chr8_4:24402913-24402932 Msa1320130:intron 25.0%
! ATTACAACAACTTTGATCTT+AGG - chr8_4:24403452-24403471 Msa1320130:intron 25.0%
! ATTATAAGAGGGAAGACTTT+TGG + chr8_4:24400065-24400084 None:intergenic 30.0%
!!! TTCCTACACTTTTTCAATGA+TGG - chr8_4:24400307-24400326 Msa1320130:three_prime_UTR 30.0%
! TACCATCATTGAAAAAGTGT+AGG + chr8_4:24400312-24400331 None:intergenic 30.0%
!!! ACTTTTTCAATGATGGTAGA+GGG - chr8_4:24400314-24400333 Msa1320130:three_prime_UTR 30.0%
GGGAAGAACAATGTATTAAT+GGG - chr8_4:24400485-24400504 Msa1320130:CDS 30.0%
AGTGTGTAATGTAATAGAAC+AGG - chr8_4:24400570-24400589 Msa1320130:CDS 30.0%
!!! TAGTCTGTTTTGATTCGATA+AGG + chr8_4:24400622-24400641 None:intergenic 30.0%
! AACTCATGTCATTTAGAAGT+TGG - chr8_4:24400971-24400990 Msa1320130:intron 30.0%
ATTATGTACTGTCCTCTAAA+GGG - chr8_4:24401358-24401377 Msa1320130:intron 30.0%
TAAATATCCACCATTCAAAC+AGG + chr8_4:24401432-24401451 None:intergenic 30.0%
AAGATCTTTGTTGAAATCTC+TGG - chr8_4:24401451-24401470 Msa1320130:intron 30.0%
TTTGTCATTGTTTCACTGAA+TGG - chr8_4:24401604-24401623 Msa1320130:intron 30.0%
TTGTCATTGTTTCACTGAAT+GGG - chr8_4:24401605-24401624 Msa1320130:intron 30.0%
! AAAATGCAAAGACGATTTTG+TGG + chr8_4:24401649-24401668 None:intergenic 30.0%
GTATACAAAATCACATCCTT+GGG + chr8_4:24401690-24401709 None:intergenic 30.0%
! CAAGGATGTGATTTTGTATA+CGG - chr8_4:24401689-24401708 Msa1320130:intron 30.0%
GAAAATGAAAACAGGAAACA+TGG + chr8_4:24401898-24401917 None:intergenic 30.0%
TCTAGAAACAATACAAACAG+TGG + chr8_4:24402030-24402049 None:intergenic 30.0%
CAATTGTGATAGTAAGAAGA+TGG + chr8_4:24402077-24402096 None:intergenic 30.0%
! ATGCCAAAGATACTTTTCTA+AGG - chr8_4:24402377-24402396 Msa1320130:intron 30.0%
! TGCCAAAGATACTTTTCTAA+GGG - chr8_4:24402378-24402397 Msa1320130:intron 30.0%
!! TTCCCTTAGAAAAGTATCTT+TGG + chr8_4:24402383-24402402 None:intergenic 30.0%
ACCTTCTATTACAGAAGAAA+GGG - chr8_4:24402420-24402439 Msa1320130:intron 30.0%
ATTACAGAAGAAAGGGATAA+TGG - chr8_4:24402427-24402446 Msa1320130:intron 30.0%
TTACAGAAGAAAGGGATAAT+GGG - chr8_4:24402428-24402447 Msa1320130:intron 30.0%
!! TTTTGTTGTATTTCCGTTAC+AGG + chr8_4:24402474-24402493 None:intergenic 30.0%
TTACTATGAGTTAGTTAGCT+AGG + chr8_4:24402535-24402554 None:intergenic 30.0%
TACACAACATGTGAAGAATT+CGG + chr8_4:24402769-24402788 None:intergenic 30.0%
GTGTATGTACATCTTATGTA+TGG - chr8_4:24402942-24402961 Msa1320130:intron 30.0%
TCAAGTATGAATGGTTTACT+AGG - chr8_4:24403355-24403374 Msa1320130:intron 30.0%
!! AAATAGCATCGACTTATCAA+GGG + chr8_4:24403393-24403412 None:intergenic 30.0%
!! AAAATAGCATCGACTTATCA+AGG + chr8_4:24403394-24403413 None:intergenic 30.0%
! TTGTTTCCTTGTTTGTAGAA+TGG - chr8_4:24403606-24403625 Msa1320130:five_prime_UTR 30.0%
AAACTGGTTGAAGTAATTTG+AGG + chr8_4:24403632-24403651 None:intergenic 30.0%
!!! CACTTTTTCAATGATGGTAG+AGG - chr8_4:24400313-24400332 Msa1320130:three_prime_UTR 35.0%
CACGCAAATCATCAAAAAGA+GGG + chr8_4:24400422-24400441 None:intergenic 35.0%
ACACGCAAATCATCAAAAAG+AGG + chr8_4:24400423-24400442 None:intergenic 35.0%
TCGTGTGATTAATCGTTAGT+CGG - chr8_4:24400462-24400481 Msa1320130:CDS 35.0%
GGGGAAGAACAATGTATTAA+TGG - chr8_4:24400484-24400503 Msa1320130:CDS 35.0%
!! AACAACAACCAAGCCTTATT+CGG - chr8_4:24400519-24400538 Msa1320130:CDS 35.0%
CGAGATAAAGTCTCTATAAG+AGG + chr8_4:24400893-24400912 None:intergenic 35.0%
AGTTTAACAGGACAAAGAGA+TGG - chr8_4:24400933-24400952 Msa1320130:intron 35.0%
GTACAGTATACTATGAAGCA+TGG - chr8_4:24401080-24401099 Msa1320130:intron 35.0%
TCTATACTGTCTATGAAGCA+CGG + chr8_4:24401227-24401246 None:intergenic 35.0%
CATTATGTACTGTCCTCTAA+AGG - chr8_4:24401357-24401376 Msa1320130:intron 35.0%
GTAACGTAAAATCCCTTTAG+AGG + chr8_4:24401373-24401392 None:intergenic 35.0%
ACTTGAAACTCCTGTTTGAA+TGG - chr8_4:24401419-24401438 Msa1320130:intron 35.0%
!! TTGAAATCTCTGGTTGATGA+TGG - chr8_4:24401461-24401480 Msa1320130:intron 35.0%
CGTATACAAAATCACATCCT+TGG + chr8_4:24401691-24401710 None:intergenic 35.0%
ATAGTAAGAAGATGGAAGAC+TGG + chr8_4:24402069-24402088 None:intergenic 35.0%
!! GCTAACATCATATAACGGAT+AGG + chr8_4:24402249-24402268 None:intergenic 35.0%
GCGTTGCTAACATCATATAA+CGG + chr8_4:24402254-24402273 None:intergenic 35.0%
!! GATACTTTTCTAAGGGAAGA+AGG - chr8_4:24402385-24402404 Msa1320130:intron 35.0%
GACCTTCTATTACAGAAGAA+AGG - chr8_4:24402419-24402438 Msa1320130:intron 35.0%
TCCCTTTCTTCTGTAATAGA+AGG + chr8_4:24402424-24402443 None:intergenic 35.0%
AGTAACATAATTCGTGCACT+AGG - chr8_4:24402550-24402569 Msa1320130:intron 35.0%
AATCAGCGTTATCCTTTCTT+TGG + chr8_4:24402687-24402706 None:intergenic 35.0%
AAAGAAAGGATAACGCTGAT+TGG - chr8_4:24402686-24402705 Msa1320130:intron 35.0%
! ACGCTGATTGGTGAAAAAAA+CGG - chr8_4:24402698-24402717 Msa1320130:intron 35.0%
ACATAATTCGCAATTCGCAT+TGG + chr8_4:24402812-24402831 None:intergenic 35.0%
ACAACTGACCATCATATGTT+AGG + chr8_4:24402924-24402943 None:intergenic 35.0%
!! GTTCCACATAAAAGTGTTCA+TGG + chr8_4:24403133-24403152 None:intergenic 35.0%
!! TTACAAAGAGGTTAGCTTGT+GGG - chr8_4:24403217-24403236 Msa1320130:intron 35.0%
ACAGTAGAGTAAATGGTAGA+AGG + chr8_4:24403270-24403289 None:intergenic 35.0%
CTACCATTTACTCTACTGTA+AGG - chr8_4:24403271-24403290 Msa1320130:intron 35.0%
! AAGGCTCTTTTATGAGTCAA+AGG - chr8_4:24403290-24403309 Msa1320130:intron 35.0%
AACCACTGTTCAAGTATGAA+TGG - chr8_4:24403346-24403365 Msa1320130:intron 35.0%
AACCATTCATACTTGAACAG+TGG + chr8_4:24403351-24403370 None:intergenic 35.0%
!! AATAGCATCGACTTATCAAG+GGG + chr8_4:24403392-24403411 None:intergenic 35.0%
ACAACAACTTTGATCTTAGG+TGG - chr8_4:24403455-24403474 Msa1320130:intron 35.0%
GTGGTGTGTAATAGTGTAAA+TGG - chr8_4:24403474-24403493 Msa1320130:intron 35.0%
! ATTCTCCTTTTATGTCTCAG+AGG - chr8_4:24403506-24403525 Msa1320130:five_prime_UTR 35.0%
! TTCTCCTTTTATGTCTCAGA+GGG - chr8_4:24403507-24403526 Msa1320130:five_prime_UTR 35.0%
! TACACACCCTTTTGGATATT+TGG - chr8_4:24403541-24403560 Msa1320130:five_prime_UTR 35.0%
ACACGACCAAATATCCAAAA+GGG + chr8_4:24403550-24403569 None:intergenic 35.0%
TACACGACCAAATATCCAAA+AGG + chr8_4:24403551-24403570 None:intergenic 35.0%
!! ACTCCTTTCGCTTTTGTTTG+AGG - chr8_4:24400195-24400214 Msa1320130:three_prime_UTR 40.0%
TGACCTCAAACAAAAGCGAA+AGG + chr8_4:24400201-24400220 None:intergenic 40.0%
TTCAATGATGGTAGAGGGTT+AGG - chr8_4:24400319-24400338 Msa1320130:three_prime_UTR 40.0%
CGTGTGATTAATCGTTAGTC+GGG - chr8_4:24400463-24400482 Msa1320130:CDS 40.0%
GTGTGATTAATCGTTAGTCG+GGG - chr8_4:24400464-24400483 Msa1320130:CDS 40.0%
TGTGATTAATCGTTAGTCGG+GGG - chr8_4:24400465-24400484 Msa1320130:CDS 40.0%
ATTCCTGTTTACACTGCAGT+AGG - chr8_4:24400765-24400784 Msa1320130:intron 40.0%
TTTCCTACTGCAGTGTAAAC+AGG + chr8_4:24400771-24400790 None:intergenic 40.0%
TAACAGGACAAAGAGATGGT+AGG - chr8_4:24400937-24400956 Msa1320130:intron 40.0%
TGAAACTCCTGTTTGAATGG+TGG - chr8_4:24401422-24401441 Msa1320130:intron 40.0%
TGGTCTAGTTGGAAGAAACT+AGG - chr8_4:24401723-24401742 Msa1320130:intron 40.0%
TTCACATTGAGCTTCGCAAA+TGG - chr8_4:24401757-24401776 Msa1320130:intron 40.0%
TCACATTGAGCTTCGCAAAT+GGG - chr8_4:24401758-24401777 Msa1320130:intron 40.0%
ACAGGAAACATGGAAGCAAA+CGG + chr8_4:24401888-24401907 None:intergenic 40.0%
CTTGTCGGAACTAAAGCAAT+TGG + chr8_4:24402208-24402227 None:intergenic 40.0%
ACATCGTCACACAAACTTGT+CGG + chr8_4:24402223-24402242 None:intergenic 40.0%
!! GAAGAAAGGGATAATGGGTA+AGG - chr8_4:24402433-24402452 Msa1320130:intron 40.0%
CCTTAGCAACTAACCTGTAA+CGG - chr8_4:24402458-24402477 Msa1320130:intron 40.0%
GAGTTAGTTAGCTAGGTTCA+AGG + chr8_4:24402528-24402547 None:intergenic 40.0%
TATGTAGCAGTACCCGAATT+CGG + chr8_4:24402612-24402631 None:intergenic 40.0%
TTGAGATGCATCAATTCACG+TGG - chr8_4:24402632-24402651 Msa1320130:intron 40.0%
!! TGTTTGCTGTTGTAGATTGC+TGG - chr8_4:24403033-24403052 Msa1320130:intron 40.0%
!! CACATAAAAGTGTTCATGGC+TGG + chr8_4:24403129-24403148 None:intergenic 40.0%
! CAGCCATGAACACTTTTATG+TGG - chr8_4:24403127-24403146 Msa1320130:intron 40.0%
AGAAATACCAAGCTCGTCAA+TGG + chr8_4:24403184-24403203 None:intergenic 40.0%
TCATTCCACCTGTTACAAAG+AGG - chr8_4:24403205-24403224 Msa1320130:intron 40.0%
CAAGCTAACCTCTTTGTAAC+AGG + chr8_4:24403216-24403235 None:intergenic 40.0%
!! GTTACAAAGAGGTTAGCTTG+TGG - chr8_4:24403216-24403235 Msa1320130:intron 40.0%
!! TACAAAGAGGTTAGCTTGTG+GGG - chr8_4:24403218-24403237 Msa1320130:intron 40.0%
GAGCCTTACAGTAGAGTAAA+TGG + chr8_4:24403277-24403296 None:intergenic 40.0%
! GGTTTGTATACACACCCTTT+TGG - chr8_4:24403533-24403552 Msa1320130:five_prime_UTR 40.0%
TTGAGGCCATTCTACAAACA+AGG + chr8_4:24403615-24403634 None:intergenic 40.0%
ACAAAACACGACACACGAGA+CGG - chr8_4:24400146-24400165 Msa1320130:three_prime_UTR 45.0%
!! CAACCAAGCCTTATTCGGAT+CGG - chr8_4:24400524-24400543 Msa1320130:CDS 45.0%
CTATGTAGCCGATCCGAATA+AGG + chr8_4:24400535-24400554 None:intergenic 45.0%
!! AGAACAGGTAAAAAGCGTCG+AGG - chr8_4:24400585-24400604 Msa1320130:CDS 45.0%
GCACAAATCTCACTGTCGAT+AGG + chr8_4:24400654-24400673 None:intergenic 45.0%
! AAGAGAGTGTGAGTCCTACT+AGG - chr8_4:24400712-24400731 Msa1320130:intron 45.0%
! AGAGAGTGTGAGTCCTACTA+GGG - chr8_4:24400713-24400732 Msa1320130:intron 45.0%
AAAGAGATGGTAGGTGGCTT+TGG - chr8_4:24400946-24400965 Msa1320130:intron 45.0%
!! CATATTTGTCGGTGTCGTGT+CGG + chr8_4:24401124-24401143 None:intergenic 45.0%
CAACTGAACTCACAGGTTCT+GGG + chr8_4:24401491-24401510 None:intergenic 45.0%
TTCTCTCCAACTGAACTCAC+AGG + chr8_4:24401498-24401517 None:intergenic 45.0%
AGTAAAATCCGAGGCTTCCT+GGG + chr8_4:24401543-24401562 None:intergenic 45.0%
CTAGTAGCGAGTAAAATCCG+AGG + chr8_4:24401552-24401571 None:intergenic 45.0%
! CGGATTTTACTCGCTACTAG+TGG - chr8_4:24401552-24401571 Msa1320130:intron 45.0%
!! TACTAGTGGAAGTGTTGCTG+CGG - chr8_4:24401566-24401585 Msa1320130:intron 45.0%
TGAAAGAACAGCGATTCCCA+AGG - chr8_4:24401671-24401690 Msa1320130:intron 45.0%
TGTATACGGACGACGATGAA+TGG - chr8_4:24401703-24401722 Msa1320130:intron 45.0%
ACGACGATGAATGGTCTAGT+TGG - chr8_4:24401712-24401731 Msa1320130:intron 45.0%
!! TCGCAAATGGGCTGATATCA+TGG - chr8_4:24401770-24401789 Msa1320130:intron 45.0%
CATGGTCATTGCTCCATTGT+CGG - chr8_4:24401788-24401807 Msa1320130:intron 45.0%
CAGGAAACATGGAAGCAAAC+GGG + chr8_4:24401887-24401906 None:intergenic 45.0%
! AAGAAGATGGAAGACTGGCT+AGG + chr8_4:24402064-24402083 None:intergenic 45.0%
GATGTTAGCAACGCAAGAGT+TGG - chr8_4:24402260-24402279 Msa1320130:five_prime_UTR 45.0%
! CCGTTACAGGTTAGTTGCTA+AGG + chr8_4:24402461-24402480 None:intergenic 45.0%
!! CAATTCGCGAGTACCGAATT+CGG - chr8_4:24402596-24402615 Msa1320130:intron 45.0%
!! AATTCGCGAGTACCGAATTC+GGG - chr8_4:24402597-24402616 Msa1320130:intron 45.0%
TTTGCACAAACTGCCATACC+AGG - chr8_4:24402657-24402676 Msa1320130:intron 45.0%
TTTCTTTGGTACGCCTGGTA+TGG + chr8_4:24402673-24402692 None:intergenic 45.0%
ATACCAGGCGTACCAAAGAA+AGG - chr8_4:24402672-24402691 Msa1320130:intron 45.0%
TATCCTTTCTTTGGTACGCC+TGG + chr8_4:24402678-24402697 None:intergenic 45.0%
! TCGCAATTCGCATTGGTACA+CGG + chr8_4:24402805-24402824 None:intergenic 45.0%
! TTGCTGTTGTAGATTGCTGG+AGG - chr8_4:24403036-24403055 Msa1320130:intron 45.0%
TGCTGTTGTAGATTGCTGGA+GGG - chr8_4:24403037-24403056 Msa1320130:intron 45.0%
ATGGCTGGTGCAACAAAGAA+TGG + chr8_4:24403114-24403133 None:intergenic 45.0%
GGAACAATCCTTTCACAGAG+CGG - chr8_4:24403148-24403167 Msa1320130:intron 45.0%
GCTAACCTCTTTGTAACAGG+TGG + chr8_4:24403213-24403232 None:intergenic 45.0%
!! AGGTTAGCTTGTGGGGATTA+TGG - chr8_4:24403225-24403244 Msa1320130:intron 45.0%
GGATTATGGCAATGGTGCAA+TGG - chr8_4:24403239-24403258 Msa1320130:intron 45.0%
TATGAGTCAAAGGCTCAGCA+AGG - chr8_4:24403300-24403319 Msa1320130:intron 45.0%
TCAAAGGCTCAGCAAGGTAA+CGG - chr8_4:24403306-24403325 Msa1320130:intron 45.0%
!! CTCACCCTCTGAGACATAAA+AGG + chr8_4:24403514-24403533 None:intergenic 45.0%
! CTTTTATGTCTCAGAGGGTG+AGG - chr8_4:24403512-24403531 Msa1320130:five_prime_UTR 45.0%
AGACCCAAACCAATACCACC+AGG - chr8_4:24400039-24400058 Msa1320130:three_prime_UTR 50.0%
!! TGGCCTGGTGGTATTGGTTT+GGG + chr8_4:24400045-24400064 None:intergenic 50.0%
!! TTGGCCTGGTGGTATTGGTT+TGG + chr8_4:24400046-24400065 None:intergenic 50.0%
! GACTTTTGGCCTGGTGGTAT+TGG + chr8_4:24400051-24400070 None:intergenic 50.0%
! AAGAGGGAAGACTTTTGGCC+TGG + chr8_4:24400060-24400079 None:intergenic 50.0%
CAAAACACGACACACGAGAC+GGG - chr8_4:24400147-24400166 Msa1320130:three_prime_UTR 50.0%
ACACACGAGACGGGAAACAA+GGG - chr8_4:24400156-24400175 Msa1320130:three_prime_UTR 50.0%
TAGCCGATCCGAATAAGGCT+TGG + chr8_4:24400530-24400549 None:intergenic 50.0%
! GAGAGTGTGAGTCCTACTAG+GGG - chr8_4:24400714-24400733 Msa1320130:intron 50.0%
! CACTTGAAGCATCCCCTAGT+AGG + chr8_4:24400729-24400748 None:intergenic 50.0%
CTACTAGGGGATGCTTCAAG+TGG - chr8_4:24400727-24400746 Msa1320130:intron 50.0%
CAGGACAAAGAGATGGTAGG+TGG - chr8_4:24400940-24400959 Msa1320130:intron 50.0%
ACGGATACAGACACGTACAC+CGG + chr8_4:24401208-24401227 None:intergenic 50.0%
CCAACTGAACTCACAGGTTC+TGG + chr8_4:24401492-24401511 None:intergenic 50.0%
CCAGAACCTGTGAGTTCAGT+TGG - chr8_4:24401489-24401508 Msa1320130:intron 50.0%
TAAAATCCGAGGCTTCCTGG+GGG + chr8_4:24401541-24401560 None:intergenic 50.0%
GTAAAATCCGAGGCTTCCTG+GGG + chr8_4:24401542-24401561 None:intergenic 50.0%
GAGTAAAATCCGAGGCTTCC+TGG + chr8_4:24401544-24401563 None:intergenic 50.0%
!! CCAAGAGTGTTTGCCGACAA+TGG + chr8_4:24401804-24401823 None:intergenic 50.0%
CCATTGTCGGCAAACACTCT+TGG - chr8_4:24401801-24401820 Msa1320130:intron 50.0%
! CGAATTGGTTCACGCCTTCA+CGG + chr8_4:24402583-24402602 None:intergenic 50.0%
CGAATTCGGTACTCGCGAAT+TGG + chr8_4:24402598-24402617 None:intergenic 50.0%
! TTCGCATTGGTACACGGTAC+AGG + chr8_4:24402799-24402818 None:intergenic 50.0%
!! GAATGGCTTGCTATAGTCCC+AGG + chr8_4:24403097-24403116 None:intergenic 50.0%
ATGAGATGCCGCTCTGTGAA+AGG + chr8_4:24403159-24403178 None:intergenic 50.0%
CTCATCTCCATTGACGAGCT+TGG - chr8_4:24403174-24403193 Msa1320130:intron 50.0%
GCTTGTGGGGATTATGGCAA+TGG - chr8_4:24403231-24403250 Msa1320130:intron 50.0%
! AGGGAAGACTTTTGGCCTGG+TGG + chr8_4:24400057-24400076 None:intergenic 55.0%
GACACACGAGACGGGAAACA+AGG - chr8_4:24400155-24400174 Msa1320130:three_prime_UTR 55.0%
!! TGAACTCACAGGTTCTGGGC+TGG + chr8_4:24401487-24401506 None:intergenic 55.0%
GTCGGCAAACACTCTTGGCA+AGG - chr8_4:24401806-24401825 Msa1320130:intron 55.0%
GAGAGCAAGCTCTTCTGCAC+TGG - chr8_4:24402327-24402346 Msa1320130:intron 55.0%
CACTAGGCAGAGAACCGTGA+AGG - chr8_4:24402566-24402585 Msa1320130:intron 55.0%
TGGTACACGGTACAGGTTCG+CGG + chr8_4:24402792-24402811 None:intergenic 55.0%
TCACATGTATCGTGCGAGCC+TGG - chr8_4:24403076-24403095 Msa1320130:intron 55.0%
CACATGTATCGTGCGAGCCT+GGG - chr8_4:24403077-24403096 Msa1320130:intron 55.0%
GTGTCGGAGTTGGACACGCT+CGG + chr8_4:24401108-24401127 None:intergenic 60.0%
!! TGTCGGTGTCGTGTCGGAGT+TGG + chr8_4:24401118-24401137 None:intergenic 60.0%
CGGCATGTCTGTGTCGTGTC+CGG - chr8_4:24401186-24401205 Msa1320130:intron 60.0%
CAGTGAATGCTGCTGCCCCC+AGG - chr8_4:24401523-24401542 Msa1320130:intron 65.0%
CTGCTGCCCCCAGGAAGCCT+CGG - chr8_4:24401532-24401551 Msa1320130:intron 70.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 24400021 24403675 24400021 ID=Msa1320130;Name=Msa1320130
chr8_4 mRNA 24400021 24403675 24400021 ID=Msa1320130-mRNA-1;Parent=Msa1320130;Name=Msa1320130-mRNA-1;_AED=0.01;_eAED=0.01;_QI=270|1|1|1|0.5|0.33|3|337|213
chr8_4 exon 24400021 24400648 24400021 ID=Msa1320130-mRNA-1:exon:8371;Parent=Msa1320130-mRNA-1
chr8_4 exon 24401869 24402293 24401869 ID=Msa1320130-mRNA-1:exon:8370;Parent=Msa1320130-mRNA-1
chr8_4 exon 24403480 24403675 24403480 ID=Msa1320130-mRNA-1:exon:8369;Parent=Msa1320130-mRNA-1
chr8_4 five_prime_UTR 24403480 24403675 24403480 ID=Msa1320130-mRNA-1:five_prime_utr;Parent=Msa1320130-mRNA-1
chr8_4 five_prime_UTR 24402220 24402293 24402220 ID=Msa1320130-mRNA-1:five_prime_utr;Parent=Msa1320130-mRNA-1
chr8_4 CDS 24401869 24402219 24401869 ID=Msa1320130-mRNA-1:cds;Parent=Msa1320130-mRNA-1
chr8_4 CDS 24400358 24400648 24400358 ID=Msa1320130-mRNA-1:cds;Parent=Msa1320130-mRNA-1
chr8_4 three_prime_UTR 24400021 24400357 24400021 ID=Msa1320130-mRNA-1:three_prime_utr;Parent=Msa1320130-mRNA-1
Gene Sequence

>Msa1320130

ATGATGGCCAGCCCAGAACCTGTGAGTTCAGTTGGAGAGAATATGTCAGTGAATGCTGCTGCCCCCAGGAAGCCTCGGATTTTACTCGCTACTAGTGGAAGTGTTGCTGCGGTTAAATTTGCAAATCTTTGTCATTGTTTCACTGAATGGGCAGAAGTAAGAGCTGTTGCCACAAAATCGTCTTTGCATTTTATTGAAAGAACAGCGATTCCCAAGGATGTGATTTTGTATACGGACGACGATGAATGGTCTAGTTGGAAGAAACTAGGCGATAGCGTGCTTCACATTGAGCTTCGCAAATGGGCTGATATCATGGTCATTGCTCCATTGTCGGCAAACACTCTTGGCAAGATTGCTGGAGGGTTGTGTGACAATCTACTCACATGTATCGTGCGAGCCTGGGACTATAGCAAGCCATTCTTTGTTGCACCAGCCATGAACACTTTTATGTGGAACAATCCTTTCACAGAGCGGCATCTCATCTCCATTGACGAGCTTGGTATTTCTCTCATTCCACCTGTTACAAAGAGGTTAGCTTGTGGGGATTATGGCAATGGTGCAATGGCTGAACCTTCTACCATTTACTCTACTGTAAGGCTCTTTTATGAGTCAAAGGCTCAGCAAGGTAACGGCGATGTTTAG
Protein sequence

>Msa1320130

MMASPEPVSSVGENMSVNAAAPRKPRILLATSGSVAAVKFANLCHCFTEWAEVRAVATKSSLHFIERTAIPKDVILYTDDDEWSSWKKLGDSVLHIELRKWADIMVIAPLSANTLGKIAGGLCDNLLTCIVRAWDYSKPFFVAPAMNTFMWNNPFTERHLISIDELGISLIPPVTKRLACGDYGNGAMAEPSTIYSTVRLFYESKAQQGNGDV*