AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1247650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1247650 MtrunA17_Chr8g0362671 77.778 117 24 2 6 120 15 131 2.57e-58 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 43 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGACGGCGATGGCTTTA+AGG 0.159670 8_2:+40758056 Msa1247650:CDS
TATAGAATGAACGTACTTAA+TGG 0.254199 8_2:+40758130 Msa1247650:exon
TTACATATCAGTAATACTTT+AGG 0.280945 8_2:-40758428 None:intergenic
CAAGAAACAGCTTAATAAAA+AGG 0.299718 8_2:+40757912 Msa1247650:CDS
AGAGAAGTGTTTGTAAGATT+TGG 0.302917 8_2:-40758509 None:intergenic
TACATATCAGTAATACTTTA+GGG 0.314829 8_2:-40758427 None:intergenic
ACAACAGCCTCCAAACAAAA+TGG 0.355141 8_2:+40758038 Msa1247650:CDS
GGTATATTTAACAATCCTTC+AGG 0.357992 8_2:-40757868 None:intergenic
ACCCTTCCATAGGAATTAAT+AGG 0.369668 8_2:-40758473 None:intergenic
ATGGACGGCGATGGCTTTAA+GGG 0.372258 8_2:+40758057 Msa1247650:CDS
CAACAAGTTTGGTTGATGTT+TGG 0.372722 8_2:-40758008 None:intergenic
AGCTGTTTCTTGGTCTTCAA+TGG 0.399678 8_2:-40757901 None:intergenic
TTCTTGGTCTTCAATGGTGT+TGG 0.407736 8_2:-40757895 None:intergenic
TGACCCACGTGCGATGGCAT+GGG 0.410175 8_2:+40758326 Msa1247650:three_prime_UTR
TTGTGACTCTTCAACAAGTT+TGG 0.410442 8_2:-40758019 None:intergenic
TGAATCTCACATAAAATAGT+AGG 0.427610 8_2:+40758201 Msa1247650:three_prime_UTR
CTGACCCACGTGCGATGGCA+TGG 0.434048 8_2:+40758325 Msa1247650:three_prime_UTR
GTCTTCAATGGTGTTGGCAT+TGG 0.435534 8_2:-40757889 None:intergenic
ACACTTCCTATTAATTCCTA+TGG 0.456644 8_2:+40758467 Msa1247650:three_prime_UTR
TTCCTATTAATTCCTATGGA+AGG 0.465675 8_2:+40758471 Msa1247650:three_prime_UTR
CCCTTCTGACCCACGTGCGA+TGG 0.474866 8_2:+40758320 Msa1247650:three_prime_UTR
GCCATCGCACGTGGGTCAGA+AGG 0.477624 8_2:-40758321 None:intergenic
TCCTATTAATTCCTATGGAA+GGG 0.496799 8_2:+40758472 Msa1247650:three_prime_UTR
CCTATGGGTGTCGCCATGGC+GGG 0.498523 8_2:+40756813 None:intergenic
TAGAAAGCAGGTAGATAAGA+TGG 0.505727 8_2:+40757843 Msa1247650:CDS
TATCCCCATGCCATCGCACG+TGG 0.519207 8_2:-40758330 None:intergenic
TTTACTCAATAAAAGAGGGA+GGG 0.546202 8_2:+40758237 Msa1247650:three_prime_UTR
AGGGACAAATTACAAGAGCT+AGG 0.555935 8_2:-40758408 None:intergenic
ACACTATAAGCAATTTGTGA+AGG 0.563084 8_2:-40756840 None:intergenic
GACCCACGTGCGATGGCATG+GGG 0.566110 8_2:+40758327 Msa1247650:three_prime_UTR
CCATCGCACGTGGGTCAGAA+GGG 0.578348 8_2:-40758320 None:intergenic
ATGTTGATTAATAACAGTCA+TGG 0.580097 8_2:-40756890 None:intergenic
CACTATAAGCAATTTGTGAA+GGG 0.582848 8_2:-40756839 None:intergenic
CCAAACAAAATGGACGGCGA+TGG 0.583460 8_2:+40758048 Msa1247650:CDS
GATCAGTACACCACATGTTG+TGG 0.596807 8_2:-40758377 None:intergenic
GCTCATTGCAGGTAGAAAGC+AGG 0.600091 8_2:+40757831 Msa1247650:intron
TTGTGAAGGGATGCCCGCCA+TGG 0.610784 8_2:-40756826 None:intergenic
CAGCCTCCAAACAAAATGGA+CGG 0.632337 8_2:+40758042 Msa1247650:CDS
GTAGATAAGATGGTGCCTGA+AGG 0.644164 8_2:+40757853 Msa1247650:CDS
GTCATGGAGGAACAGTGTGA+CGG 0.649085 8_2:-40756874 None:intergenic
TGTCAATTTGCCACAACATG+TGG 0.659668 8_2:+40758367 Msa1247650:three_prime_UTR
TTGATTAATAACAGTCATGG+AGG 0.665353 8_2:-40756887 None:intergenic
ATCCCCATGCCATCGCACGT+GGG 0.708409 8_2:-40758329 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTACTCAATAAAAGA+GGG + chr8_2:40758233-40758252 Msa1247650:three_prime_UTR 15.0%
!!! TAATTTTACTCAATAAAAGA+GGG + chr8_2:40758233-40758252 Msa1247650:three_prime_UTR 15.0%
!!! AGTATTTGGTTCTTTATTAA+TGG + chr8_2:40756951-40756970 Msa1247650:intron 20.0%
!!! GTATTTGGTTCTTTATTAAT+GGG + chr8_2:40756952-40756971 Msa1247650:intron 20.0%
!!! AAAACTCTTACAATTGTTTT+TGG + chr8_2:40756975-40756994 Msa1247650:intron 20.0%
!! TAACAAAAGAAAAGTTGAAA+AGG - chr8_2:40757096-40757115 None:intergenic 20.0%
!!! TTTCTTTTGTTATAAGTGAT+CGG + chr8_2:40757104-40757123 Msa1247650:intron 20.0%
!! TGGTTAAATTATAGACTATA+TGG - chr8_2:40757187-40757206 None:intergenic 20.0%
!!! TTATTTTTGTATTAACCCTT+CGG - chr8_2:40757363-40757382 None:intergenic 20.0%
!! TAATTGAATATAATCCACAT+AGG - chr8_2:40757748-40757767 None:intergenic 20.0%
!!! ATTCAATTTTGAAACAAAAG+TGG + chr8_2:40757945-40757964 Msa1247650:CDS 20.0%
!!! GTAATTTTACTCAATAAAAG+AGG + chr8_2:40758232-40758251 Msa1247650:three_prime_UTR 20.0%
!! TACATATCAGTAATACTTTA+GGG - chr8_2:40758430-40758449 None:intergenic 20.0%
!! TTACATATCAGTAATACTTT+AGG - chr8_2:40758431-40758450 None:intergenic 20.0%
!!! AGTATTTGGTTCTTTATTAA+TGG + chr8_2:40756951-40756970 Msa1247650:intron 20.0%
!!! GTATTTGGTTCTTTATTAAT+GGG + chr8_2:40756952-40756971 Msa1247650:intron 20.0%
!!! AAAACTCTTACAATTGTTTT+TGG + chr8_2:40756975-40756994 Msa1247650:intron 20.0%
!! TAACAAAAGAAAAGTTGAAA+AGG - chr8_2:40757096-40757115 None:intergenic 20.0%
!!! TTTCTTTTGTTATAAGTGAT+CGG + chr8_2:40757104-40757123 Msa1247650:intron 20.0%
!! TGGTTAAATTATAGACTATA+TGG - chr8_2:40757187-40757206 None:intergenic 20.0%
!!! TTATTTTTGTATTAACCCTT+CGG - chr8_2:40757363-40757382 None:intergenic 20.0%
!! TAATTGAATATAATCCACAT+AGG - chr8_2:40757748-40757767 None:intergenic 20.0%
!!! ATTCAATTTTGAAACAAAAG+TGG + chr8_2:40757945-40757964 Msa1247650:CDS 20.0%
!!! GTAATTTTACTCAATAAAAG+AGG + chr8_2:40758232-40758251 Msa1247650:three_prime_UTR 20.0%
!! TACATATCAGTAATACTTTA+GGG - chr8_2:40758430-40758449 None:intergenic 20.0%
!! TTACATATCAGTAATACTTT+AGG - chr8_2:40758431-40758450 None:intergenic 20.0%
!! ATGTTGATTAATAACAGTCA+TGG - chr8_2:40756893-40756912 None:intergenic 25.0%
!! TGATTAACTTCTAGTTAACT+CGG + chr8_2:40756913-40756932 Msa1247650:intron 25.0%
! TTTCAACTGAAATGAGTATT+TGG + chr8_2:40756937-40756956 Msa1247650:intron 25.0%
! AACTAATTTAAATTCACACG+AGG + chr8_2:40757036-40757055 Msa1247650:intron 25.0%
!!! TTCTTTTGTTATAAGTGATC+GGG + chr8_2:40757105-40757124 Msa1247650:intron 25.0%
!! AACTTCAGTTAAGAATCTTT+TGG + chr8_2:40757234-40757253 Msa1247650:intron 25.0%
!! ACTTCAGTTAAGAATCTTTT+GGG + chr8_2:40757235-40757254 Msa1247650:intron 25.0%
!! TTTAGTCTGGAATTGAATTT+GGG - chr8_2:40757265-40757284 None:intergenic 25.0%
!!! TTTTAGTCTGGAATTGAATT+TGG - chr8_2:40757266-40757285 None:intergenic 25.0%
!!! GATGAAAAATTGTTTTAGTC+TGG - chr8_2:40757278-40757297 None:intergenic 25.0%
!!! CTAAAGTGCATTATTGATTT+TGG - chr8_2:40757604-40757623 None:intergenic 25.0%
! GCTTCATATTATTCCATATT+GGG + chr8_2:40757779-40757798 Msa1247650:intron 25.0%
!!! CTTTTTATTAAGCTGTTTCT+TGG - chr8_2:40757914-40757933 None:intergenic 25.0%
! CAAGAAACAGCTTAATAAAA+AGG + chr8_2:40757912-40757931 Msa1247650:CDS 25.0%
! ATTAATCTTCTGCAGAAAAA+TGG + chr8_2:40758081-40758100 Msa1247650:CDS 25.0%
! TTAATCTTCTGCAGAAAAAT+GGG + chr8_2:40758082-40758101 Msa1247650:CDS 25.0%
! TATAGAATGAACGTACTTAA+TGG + chr8_2:40758130-40758149 Msa1247650:exon 25.0%
! TGAATCTCACATAAAATAGT+AGG + chr8_2:40758201-40758220 Msa1247650:three_prime_UTR 25.0%
!! ATGTTGATTAATAACAGTCA+TGG - chr8_2:40756893-40756912 None:intergenic 25.0%
!! TGATTAACTTCTAGTTAACT+CGG + chr8_2:40756913-40756932 Msa1247650:intron 25.0%
! TTTCAACTGAAATGAGTATT+TGG + chr8_2:40756937-40756956 Msa1247650:intron 25.0%
! AACTAATTTAAATTCACACG+AGG + chr8_2:40757036-40757055 Msa1247650:intron 25.0%
!!! TTCTTTTGTTATAAGTGATC+GGG + chr8_2:40757105-40757124 Msa1247650:intron 25.0%
!! AACTTCAGTTAAGAATCTTT+TGG + chr8_2:40757234-40757253 Msa1247650:intron 25.0%
!! ACTTCAGTTAAGAATCTTTT+GGG + chr8_2:40757235-40757254 Msa1247650:intron 25.0%
!! TTTAGTCTGGAATTGAATTT+GGG - chr8_2:40757265-40757284 None:intergenic 25.0%
!!! TTTTAGTCTGGAATTGAATT+TGG - chr8_2:40757266-40757285 None:intergenic 25.0%
!!! GATGAAAAATTGTTTTAGTC+TGG - chr8_2:40757278-40757297 None:intergenic 25.0%
!!! CTAAAGTGCATTATTGATTT+TGG - chr8_2:40757604-40757623 None:intergenic 25.0%
! GCTTCATATTATTCCATATT+GGG + chr8_2:40757779-40757798 Msa1247650:intron 25.0%
!!! CTTTTTATTAAGCTGTTTCT+TGG - chr8_2:40757914-40757933 None:intergenic 25.0%
! CAAGAAACAGCTTAATAAAA+AGG + chr8_2:40757912-40757931 Msa1247650:CDS 25.0%
! ATTAATCTTCTGCAGAAAAA+TGG + chr8_2:40758081-40758100 Msa1247650:CDS 25.0%
! TTAATCTTCTGCAGAAAAAT+GGG + chr8_2:40758082-40758101 Msa1247650:CDS 25.0%
! TATAGAATGAACGTACTTAA+TGG + chr8_2:40758130-40758149 Msa1247650:exon 25.0%
! TGAATCTCACATAAAATAGT+AGG + chr8_2:40758201-40758220 Msa1247650:three_prime_UTR 25.0%
CACTATAAGCAATTTGTGAA+GGG - chr8_2:40756842-40756861 None:intergenic 30.0%
ACACTATAAGCAATTTGTGA+AGG - chr8_2:40756843-40756862 None:intergenic 30.0%
TTGATTAATAACAGTCATGG+AGG - chr8_2:40756890-40756909 None:intergenic 30.0%
! GATTAACTTCTAGTTAACTC+GGG + chr8_2:40756914-40756933 Msa1247650:intron 30.0%
ACGAGGAACTGTTAATTAAA+GGG + chr8_2:40757053-40757072 Msa1247650:intron 30.0%
GTCTATAATTTAACCAAGTG+TGG + chr8_2:40757191-40757210 Msa1247650:intron 30.0%
ATGACAACTATCTTTGTGAT+TGG + chr8_2:40757567-40757586 Msa1247650:intron 30.0%
!!! AAGTGCATTATTGATTTTGG+AGG - chr8_2:40757601-40757620 None:intergenic 30.0%
TAGAACAGTCTAATTACACA+TGG - chr8_2:40757697-40757716 None:intergenic 30.0%
CGCTTCATATTATTCCATAT+TGG + chr8_2:40757778-40757797 Msa1247650:intron 30.0%
AGCACAGTAATGATATTTGA+GGG - chr8_2:40757814-40757833 None:intergenic 30.0%
GGTATATTTAACAATCCTTC+AGG - chr8_2:40757871-40757890 None:intergenic 30.0%
! TTTTACTCAATAAAAGAGGG+AGG + chr8_2:40758236-40758255 Msa1247650:three_prime_UTR 30.0%
TTTACTCAATAAAAGAGGGA+GGG + chr8_2:40758237-40758256 Msa1247650:three_prime_UTR 30.0%
ACACTTCCTATTAATTCCTA+TGG + chr8_2:40758467-40758486 Msa1247650:three_prime_UTR 30.0%
TTCCTATTAATTCCTATGGA+AGG + chr8_2:40758471-40758490 Msa1247650:three_prime_UTR 30.0%
TCCTATTAATTCCTATGGAA+GGG + chr8_2:40758472-40758491 Msa1247650:three_prime_UTR 30.0%
!! AGAGAAGTGTTTGTAAGATT+TGG - chr8_2:40758512-40758531 None:intergenic 30.0%
CACTATAAGCAATTTGTGAA+GGG - chr8_2:40756842-40756861 None:intergenic 30.0%
ACACTATAAGCAATTTGTGA+AGG - chr8_2:40756843-40756862 None:intergenic 30.0%
TTGATTAATAACAGTCATGG+AGG - chr8_2:40756890-40756909 None:intergenic 30.0%
! GATTAACTTCTAGTTAACTC+GGG + chr8_2:40756914-40756933 Msa1247650:intron 30.0%
ACGAGGAACTGTTAATTAAA+GGG + chr8_2:40757053-40757072 Msa1247650:intron 30.0%
GTCTATAATTTAACCAAGTG+TGG + chr8_2:40757191-40757210 Msa1247650:intron 30.0%
ATGACAACTATCTTTGTGAT+TGG + chr8_2:40757567-40757586 Msa1247650:intron 30.0%
!!! AAGTGCATTATTGATTTTGG+AGG - chr8_2:40757601-40757620 None:intergenic 30.0%
TAGAACAGTCTAATTACACA+TGG - chr8_2:40757697-40757716 None:intergenic 30.0%
CGCTTCATATTATTCCATAT+TGG + chr8_2:40757778-40757797 Msa1247650:intron 30.0%
AGCACAGTAATGATATTTGA+GGG - chr8_2:40757814-40757833 None:intergenic 30.0%
GGTATATTTAACAATCCTTC+AGG - chr8_2:40757871-40757890 None:intergenic 30.0%
! TTTTACTCAATAAAAGAGGG+AGG + chr8_2:40758236-40758255 Msa1247650:three_prime_UTR 30.0%
TTTACTCAATAAAAGAGGGA+GGG + chr8_2:40758237-40758256 Msa1247650:three_prime_UTR 30.0%
ACACTTCCTATTAATTCCTA+TGG + chr8_2:40758467-40758486 Msa1247650:three_prime_UTR 30.0%
TTCCTATTAATTCCTATGGA+AGG + chr8_2:40758471-40758490 Msa1247650:three_prime_UTR 30.0%
TCCTATTAATTCCTATGGAA+GGG + chr8_2:40758472-40758491 Msa1247650:three_prime_UTR 30.0%
!! AGAGAAGTGTTTGTAAGATT+TGG - chr8_2:40758512-40758531 None:intergenic 30.0%
CACGAGGAACTGTTAATTAA+AGG + chr8_2:40757052-40757071 Msa1247650:intron 35.0%
ATAATGCGTAGTTGAGCATA+TGG + chr8_2:40757420-40757439 Msa1247650:intron 35.0%
TAATGCGTAGTTGAGCATAT+GGG + chr8_2:40757421-40757440 Msa1247650:intron 35.0%
ACCAATGCATCATCAAACAA+GGG - chr8_2:40757471-40757490 None:intergenic 35.0%
GACACAGTATATCAACAACA+AGG - chr8_2:40757518-40757537 None:intergenic 35.0%
TTGTCATAGTCACAAGTCAA+TGG - chr8_2:40757554-40757573 None:intergenic 35.0%
!!! TGCATTATTGATTTTGGAGG+AGG - chr8_2:40757598-40757617 None:intergenic 35.0%
TATTATTCCATATTGGGACG+AGG + chr8_2:40757785-40757804 Msa1247650:intron 35.0%
GAGCACAGTAATGATATTTG+AGG - chr8_2:40757815-40757834 None:intergenic 35.0%
TAGAAAGCAGGTAGATAAGA+TGG + chr8_2:40757843-40757862 Msa1247650:CDS 35.0%
! CAACAAGTTTGGTTGATGTT+TGG - chr8_2:40758011-40758030 None:intergenic 35.0%
TTGTGACTCTTCAACAAGTT+TGG - chr8_2:40758022-40758041 None:intergenic 35.0%
ACCCTTCCATAGGAATTAAT+AGG - chr8_2:40758476-40758495 None:intergenic 35.0%
! TCATTTTCTAACCCTTCCAT+AGG - chr8_2:40758486-40758505 None:intergenic 35.0%
CACGAGGAACTGTTAATTAA+AGG + chr8_2:40757052-40757071 Msa1247650:intron 35.0%
ATAATGCGTAGTTGAGCATA+TGG + chr8_2:40757420-40757439 Msa1247650:intron 35.0%
TAATGCGTAGTTGAGCATAT+GGG + chr8_2:40757421-40757440 Msa1247650:intron 35.0%
ACCAATGCATCATCAAACAA+GGG - chr8_2:40757471-40757490 None:intergenic 35.0%
GACACAGTATATCAACAACA+AGG - chr8_2:40757518-40757537 None:intergenic 35.0%
TTGTCATAGTCACAAGTCAA+TGG - chr8_2:40757554-40757573 None:intergenic 35.0%
!!! TGCATTATTGATTTTGGAGG+AGG - chr8_2:40757598-40757617 None:intergenic 35.0%
TATTATTCCATATTGGGACG+AGG + chr8_2:40757785-40757804 Msa1247650:intron 35.0%
GAGCACAGTAATGATATTTG+AGG - chr8_2:40757815-40757834 None:intergenic 35.0%
TAGAAAGCAGGTAGATAAGA+TGG + chr8_2:40757843-40757862 Msa1247650:CDS 35.0%
! CAACAAGTTTGGTTGATGTT+TGG - chr8_2:40758011-40758030 None:intergenic 35.0%
TTGTGACTCTTCAACAAGTT+TGG - chr8_2:40758022-40758041 None:intergenic 35.0%
ACCCTTCCATAGGAATTAAT+AGG - chr8_2:40758476-40758495 None:intergenic 35.0%
! TCATTTTCTAACCCTTCCAT+AGG - chr8_2:40758486-40758505 None:intergenic 35.0%
TGGAGAAGATGAGAAAGCTT+TGG + chr8_2:40756995-40757014 Msa1247650:intron 40.0%
GGAGAAGATGAGAAAGCTTT+GGG + chr8_2:40756996-40757015 Msa1247650:intron 40.0%
TGATAATACGGAGTTCAGTC+GGG - chr8_2:40757316-40757335 None:intergenic 40.0%
CTGATAATACGGAGTTCAGT+CGG - chr8_2:40757317-40757336 None:intergenic 40.0%
! AGGGGCTTTGACTGATAATA+CGG - chr8_2:40757328-40757347 None:intergenic 40.0%
!! TCCCTTGTTTGATGATGCAT+TGG + chr8_2:40757467-40757486 Msa1247650:intron 40.0%
GACCAATGCATCATCAAACA+AGG - chr8_2:40757472-40757491 None:intergenic 40.0%
CTGATCTTCTCTCCTTACTA+TGG + chr8_2:40757720-40757739 Msa1247650:intron 40.0%
TCCTTACTATGGAACCTATG+TGG + chr8_2:40757731-40757750 Msa1247650:intron 40.0%
TCCACATAGGTTCCATAGTA+AGG - chr8_2:40757735-40757754 None:intergenic 40.0%
ATCATTACTGTGCTCATTGC+AGG + chr8_2:40757820-40757839 Msa1247650:intron 40.0%
! TTCTTGGTCTTCAATGGTGT+TGG - chr8_2:40757898-40757917 None:intergenic 40.0%
!! AGCTGTTTCTTGGTCTTCAA+TGG - chr8_2:40757904-40757923 None:intergenic 40.0%
ACAACAGCCTCCAAACAAAA+TGG + chr8_2:40758038-40758057 Msa1247650:CDS 40.0%
ATCTTCTGCAGAAAAATGGG+AGG + chr8_2:40758085-40758104 Msa1247650:CDS 40.0%
TGTCAATTTGCCACAACATG+TGG + chr8_2:40758367-40758386 Msa1247650:three_prime_UTR 40.0%
AGGGACAAATTACAAGAGCT+AGG - chr8_2:40758411-40758430 None:intergenic 40.0%
TGGAGAAGATGAGAAAGCTT+TGG + chr8_2:40756995-40757014 Msa1247650:intron 40.0%
GGAGAAGATGAGAAAGCTTT+GGG + chr8_2:40756996-40757015 Msa1247650:intron 40.0%
TGATAATACGGAGTTCAGTC+GGG - chr8_2:40757316-40757335 None:intergenic 40.0%
CTGATAATACGGAGTTCAGT+CGG - chr8_2:40757317-40757336 None:intergenic 40.0%
! AGGGGCTTTGACTGATAATA+CGG - chr8_2:40757328-40757347 None:intergenic 40.0%
!! TCCCTTGTTTGATGATGCAT+TGG + chr8_2:40757467-40757486 Msa1247650:intron 40.0%
GACCAATGCATCATCAAACA+AGG - chr8_2:40757472-40757491 None:intergenic 40.0%
CTGATCTTCTCTCCTTACTA+TGG + chr8_2:40757720-40757739 Msa1247650:intron 40.0%
TCCTTACTATGGAACCTATG+TGG + chr8_2:40757731-40757750 Msa1247650:intron 40.0%
TCCACATAGGTTCCATAGTA+AGG - chr8_2:40757735-40757754 None:intergenic 40.0%
ATCATTACTGTGCTCATTGC+AGG + chr8_2:40757820-40757839 Msa1247650:intron 40.0%
! TTCTTGGTCTTCAATGGTGT+TGG - chr8_2:40757898-40757917 None:intergenic 40.0%
!! AGCTGTTTCTTGGTCTTCAA+TGG - chr8_2:40757904-40757923 None:intergenic 40.0%
ACAACAGCCTCCAAACAAAA+TGG + chr8_2:40758038-40758057 Msa1247650:CDS 40.0%
ATCTTCTGCAGAAAAATGGG+AGG + chr8_2:40758085-40758104 Msa1247650:CDS 40.0%
TGTCAATTTGCCACAACATG+TGG + chr8_2:40758367-40758386 Msa1247650:three_prime_UTR 40.0%
AGGGACAAATTACAAGAGCT+AGG - chr8_2:40758411-40758430 None:intergenic 40.0%
TGTATTAACCCTTCGGTTCC+CGG - chr8_2:40757356-40757375 None:intergenic 45.0%
GTAGATAAGATGGTGCCTGA+AGG + chr8_2:40757853-40757872 Msa1247650:CDS 45.0%
!! GTCTTCAATGGTGTTGGCAT+TGG - chr8_2:40757892-40757911 None:intergenic 45.0%
CAGCCTCCAAACAAAATGGA+CGG + chr8_2:40758042-40758061 Msa1247650:CDS 45.0%
GTGGGTCAGAAGGGAAAAAA+AGG - chr8_2:40758314-40758333 None:intergenic 45.0%
GATCAGTACACCACATGTTG+TGG - chr8_2:40758380-40758399 None:intergenic 45.0%
TGTATTAACCCTTCGGTTCC+CGG - chr8_2:40757356-40757375 None:intergenic 45.0%
GTAGATAAGATGGTGCCTGA+AGG + chr8_2:40757853-40757872 Msa1247650:CDS 45.0%
!! GTCTTCAATGGTGTTGGCAT+TGG - chr8_2:40757892-40757911 None:intergenic 45.0%
CAGCCTCCAAACAAAATGGA+CGG + chr8_2:40758042-40758061 Msa1247650:CDS 45.0%
GTGGGTCAGAAGGGAAAAAA+AGG - chr8_2:40758314-40758333 None:intergenic 45.0%
GATCAGTACACCACATGTTG+TGG - chr8_2:40758380-40758399 None:intergenic 45.0%
GTCATGGAGGAACAGTGTGA+CGG - chr8_2:40756877-40756896 None:intergenic 50.0%
CACTTGAGTCAAGCCACACT+TGG - chr8_2:40757207-40757226 None:intergenic 50.0%
CAAGTGTGGCTTGACTCAAG+TGG + chr8_2:40757205-40757224 Msa1247650:intron 50.0%
TAATACGGAGTTCAGTCGGG+AGG - chr8_2:40757313-40757332 None:intergenic 50.0%
GCGTAGTTGAGCATATGGGT+AGG + chr8_2:40757425-40757444 Msa1247650:intron 50.0%
AGGGATTCCTCGTCCCAATA+TGG - chr8_2:40757795-40757814 None:intergenic 50.0%
GCTCATTGCAGGTAGAAAGC+AGG + chr8_2:40757831-40757850 Msa1247650:intron 50.0%
!! TCGCCGTCCATTTTGTTTGG+AGG - chr8_2:40758048-40758067 None:intergenic 50.0%
! CCATCGCCGTCCATTTTGTT+TGG - chr8_2:40758051-40758070 None:intergenic 50.0%
CCAAACAAAATGGACGGCGA+TGG + chr8_2:40758048-40758067 Msa1247650:CDS 50.0%
! AATGGACGGCGATGGCTTTA+AGG + chr8_2:40758056-40758075 Msa1247650:CDS 50.0%
!! ATGGACGGCGATGGCTTTAA+GGG + chr8_2:40758057-40758076 Msa1247650:CDS 50.0%
GTCATGGAGGAACAGTGTGA+CGG - chr8_2:40756877-40756896 None:intergenic 50.0%
CACTTGAGTCAAGCCACACT+TGG - chr8_2:40757207-40757226 None:intergenic 50.0%
CAAGTGTGGCTTGACTCAAG+TGG + chr8_2:40757205-40757224 Msa1247650:intron 50.0%
TAATACGGAGTTCAGTCGGG+AGG - chr8_2:40757313-40757332 None:intergenic 50.0%
GCGTAGTTGAGCATATGGGT+AGG + chr8_2:40757425-40757444 Msa1247650:intron 50.0%
AGGGATTCCTCGTCCCAATA+TGG - chr8_2:40757795-40757814 None:intergenic 50.0%
GCTCATTGCAGGTAGAAAGC+AGG + chr8_2:40757831-40757850 Msa1247650:intron 50.0%
!! TCGCCGTCCATTTTGTTTGG+AGG - chr8_2:40758048-40758067 None:intergenic 50.0%
! CCATCGCCGTCCATTTTGTT+TGG - chr8_2:40758051-40758070 None:intergenic 50.0%
CCAAACAAAATGGACGGCGA+TGG + chr8_2:40758048-40758067 Msa1247650:CDS 50.0%
! AATGGACGGCGATGGCTTTA+AGG + chr8_2:40758056-40758075 Msa1247650:CDS 50.0%
!! ATGGACGGCGATGGCTTTAA+GGG + chr8_2:40758057-40758076 Msa1247650:CDS 50.0%
TTAACCCTTCGGTTCCCGGA+GGG - chr8_2:40757352-40757371 None:intergenic 55.0%
ATTAACCCTTCGGTTCCCGG+AGG - chr8_2:40757353-40757372 None:intergenic 55.0%
TTAACCCTTCGGTTCCCGGA+GGG - chr8_2:40757352-40757371 None:intergenic 55.0%
ATTAACCCTTCGGTTCCCGG+AGG - chr8_2:40757353-40757372 None:intergenic 55.0%
CAGTCAAAGCCCCTTCCCTC+CGG + chr8_2:40757334-40757353 Msa1247650:intron 60.0%
AGTCAAAGCCCCTTCCCTCC+GGG + chr8_2:40757335-40757354 Msa1247650:intron 60.0%
CCATCGCACGTGGGTCAGAA+GGG - chr8_2:40758323-40758342 None:intergenic 60.0%
TGACCCACGTGCGATGGCAT+GGG + chr8_2:40758326-40758345 Msa1247650:three_prime_UTR 60.0%
ATCCCCATGCCATCGCACGT+GGG - chr8_2:40758332-40758351 None:intergenic 60.0%
TATCCCCATGCCATCGCACG+TGG - chr8_2:40758333-40758352 None:intergenic 60.0%
CAGTCAAAGCCCCTTCCCTC+CGG + chr8_2:40757334-40757353 Msa1247650:intron 60.0%
AGTCAAAGCCCCTTCCCTCC+GGG + chr8_2:40757335-40757354 Msa1247650:intron 60.0%
CCATCGCACGTGGGTCAGAA+GGG - chr8_2:40758323-40758342 None:intergenic 60.0%
TGACCCACGTGCGATGGCAT+GGG + chr8_2:40758326-40758345 Msa1247650:three_prime_UTR 60.0%
ATCCCCATGCCATCGCACGT+GGG - chr8_2:40758332-40758351 None:intergenic 60.0%
TATCCCCATGCCATCGCACG+TGG - chr8_2:40758333-40758352 None:intergenic 60.0%
CTTCGGTTCCCGGAGGGAAG+GGG - chr8_2:40757346-40757365 None:intergenic 65.0%
CCTTCGGTTCCCGGAGGGAA+GGG - chr8_2:40757347-40757366 None:intergenic 65.0%
CCTTCCCTCCGGGAACCGAA+GGG + chr8_2:40757345-40757364 Msa1247650:intron 65.0%
CCCTTCTGACCCACGTGCGA+TGG + chr8_2:40758320-40758339 Msa1247650:three_prime_UTR 65.0%
GCCATCGCACGTGGGTCAGA+AGG - chr8_2:40758324-40758343 None:intergenic 65.0%
CTGACCCACGTGCGATGGCA+TGG + chr8_2:40758325-40758344 Msa1247650:three_prime_UTR 65.0%
GACCCACGTGCGATGGCATG+GGG + chr8_2:40758327-40758346 Msa1247650:three_prime_UTR 65.0%
CTTCGGTTCCCGGAGGGAAG+GGG - chr8_2:40757346-40757365 None:intergenic 65.0%
CCTTCGGTTCCCGGAGGGAA+GGG - chr8_2:40757347-40757366 None:intergenic 65.0%
CCTTCCCTCCGGGAACCGAA+GGG + chr8_2:40757345-40757364 Msa1247650:intron 65.0%
CCCTTCTGACCCACGTGCGA+TGG + chr8_2:40758320-40758339 Msa1247650:three_prime_UTR 65.0%
GCCATCGCACGTGGGTCAGA+AGG - chr8_2:40758324-40758343 None:intergenic 65.0%
CTGACCCACGTGCGATGGCA+TGG + chr8_2:40758325-40758344 Msa1247650:three_prime_UTR 65.0%
GACCCACGTGCGATGGCATG+GGG + chr8_2:40758327-40758346 Msa1247650:three_prime_UTR 65.0%
CCCTTCCCTCCGGGAACCGA+AGG + chr8_2:40757344-40757363 Msa1247650:intron 70.0%
CCCTTCGGTTCCCGGAGGGA+AGG - chr8_2:40757348-40757367 None:intergenic 70.0%
CCCTTCCCTCCGGGAACCGA+AGG + chr8_2:40757344-40757363 Msa1247650:intron 70.0%
CCCTTCGGTTCCCGGAGGGA+AGG - chr8_2:40757348-40757367 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 40756828 40758548 40756828 ID=Msa1247650;Name=Msa1247650
chr8_2 mRNA 40756828 40758548 40756828 ID=Msa1247650-mRNA-1;Parent=Msa1247650;Name=Msa1247650-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0.5|0.5|1|1|2|414|120
chr8_2 exon 40756828 40756897 40756828 ID=Msa1247650-mRNA-1:exon:12420;Parent=Msa1247650-mRNA-1
chr8_2 exon 40757842 40758548 40757842 ID=Msa1247650-mRNA-1:exon:12421;Parent=Msa1247650-mRNA-1
chr8_2 CDS 40756828 40756897 40756828 ID=Msa1247650-mRNA-1:cds;Parent=Msa1247650-mRNA-1
chr8_2 CDS 40757842 40758134 40757842 ID=Msa1247650-mRNA-1:cds;Parent=Msa1247650-mRNA-1
chr8_2 three_prime_UTR 40758135 40758548 40758135 ID=Msa1247650-mRNA-1:three_prime_utr;Parent=Msa1247650-mRNA-1
Gene Sequence

>Msa1247650

ATGGCGGGCATCCCTTCACAAATTGCTTATAGTGTTTGTCAAAAATCCGTCACACTGTTCCTCCATGACTGTAGAAAGCAGGTAGATAAGATGGTGCCTGAAGGATTGTTAAATATACCAATGCCAACACCATTGAAGACCAAGAAACAGCTTAATAAAAAGGTTGCAGATGAATTCAATTTTGAAACAAAAGTGGAGAAAGTGACAGAAGACATGAACAATGTAAAGCAGCAGTACCAAACATCAACCAAACTTGTTGAAGAGTCACAACAGCCTCCAAACAAAATGGACGGCGATGGCTTTAAGGGAATTAATCTTCTGCAGAAAAATGGGAGGAGAGTGTTTATTCGTTCTCGTCTATAG
Protein sequence

>Msa1247650

MAGIPSQIAYSVCQKSVTLFLHDCRKQVDKMVPEGLLNIPMPTPLKTKKQLNKKVADEFNFETKVEKVTEDMNNVKQQYQTSTKLVEESQQPPNKMDGDGFKGINLLQKNGRRVFIRSRL*