AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1248540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1248540 MtrunA17_Chr4g0055401 78.035 173 35 1 2 171 129 301 3.69e-86 266
Msa1248540 MtrunA17_Chr4g0042181 27.607 163 114 2 13 171 170 332 6.37e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1248540 AT4G17616.1 48.193 166 80 2 10 171 135 298 3.71e-38 138
Msa1248540 AT1G03100.1 27.108 166 117 2 10 171 177 342 3.99e-12 63.9

Find 74 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATTGTGAAGACAGTAAT+TGG 0.189776 8_2:+42597557 Msa1248540:CDS
TTCTACATCTGAATTTAAAT+TGG 0.221172 8_2:-42595859 None:intergenic
TTGCGGATTTATAACATTAA+AGG 0.223732 8_2:+42595564 Msa1248540:five_prime_UTR
TGTGGAAATTACTTGATATT+GGG 0.256264 8_2:+42595805 Msa1248540:five_prime_UTR
ATGTGGAAATTACTTGATAT+TGG 0.266059 8_2:+42595804 Msa1248540:five_prime_UTR
TAGTAGTTTGAGGGATAAAA+AGG 0.266433 8_2:+42597625 Msa1248540:CDS
TGATCCATGTAATTTATCTT+TGG 0.299624 8_2:+42597436 Msa1248540:CDS
ACTACAATTAATCTTCATAT+TGG 0.315911 8_2:-42595499 None:intergenic
GGAGGCAGTTAATTCGTCTA+TGG 0.339461 8_2:+42595968 Msa1248540:exon
TCACACAAGCATCAAGAAAA+AGG 0.343795 8_2:-42597691 None:intergenic
AACCTAGGTATAACTGTTGC+AGG 0.355838 8_2:-42597479 None:intergenic
TTGCCGCAAGTTTGATTCAA+TGG 0.373385 8_2:+42595521 Msa1248540:five_prime_UTR
TTTCTAAATGAGTATCTTGA+AGG 0.382168 8_2:+42595366 Msa1248540:five_prime_UTR
AGGTTAAATATCAAAGTATC+TGG 0.384496 8_2:-42597671 None:intergenic
ATCATCATTGATCACTGAAA+TGG 0.387294 8_2:-42595691 None:intergenic
GTCATTCTACCATCTAGTTT+CGG 0.391713 8_2:+42595749 Msa1248540:five_prime_UTR
CTTGACTTTCCGAAACTAGA+TGG 0.401068 8_2:-42595758 None:intergenic
TAAATATTAGTGAAACATTA+CGG 0.422498 8_2:+42595304 Msa1248540:five_prime_UTR
GATGGCACAAACCCTTTGTT+AGG 0.426964 8_2:-42597506 None:intergenic
TGAGCACATGCCTAGGGGTA+TGG 0.429091 8_2:+42595653 Msa1248540:five_prime_UTR
TGTTTGTACTCTCAATCCTT+TGG 0.442113 8_2:-42595143 None:intergenic
AACTCTCACACAATTCTTAG+TGG 0.442170 8_2:+42595197 Msa1248540:five_prime_UTR
TGCCGCAAGTTTGATTCAAT+GGG 0.448344 8_2:+42595522 Msa1248540:five_prime_UTR
TGTACTCTCAATCCTTTGGC+AGG 0.451258 8_2:-42595139 None:intergenic
TTCTTTGTAGTCATGCTTTG+CGG 0.453705 8_2:+42595547 Msa1248540:five_prime_UTR
AATGAGTATCTTGAAGGAAC+AGG 0.455830 8_2:+42595372 Msa1248540:five_prime_UTR
TTGCAGATTTCGGATATGAA+TGG 0.468630 8_2:+42597803 Msa1248540:CDS
GAATTGAAATGTCATATTGA+TGG 0.474600 8_2:+42597845 Msa1248540:CDS
TAACAAGAGGAGGTGTACTT+TGG 0.474858 8_2:-42595281 None:intergenic
AGCAAGAATGTATTCTCTGC+TGG 0.493952 8_2:+42595108 None:intergenic
AACATTACGGCATGCATCTA+CGG 0.494329 8_2:+42595317 Msa1248540:five_prime_UTR
GAGCACATGCCTAGGGGTAT+GGG 0.504100 8_2:+42595654 Msa1248540:five_prime_UTR
TTAGCCAAAGATAAATTACA+TGG 0.509347 8_2:-42597440 None:intergenic
TGATTTATGAGCACATGCCT+AGG 0.520789 8_2:+42595646 Msa1248540:five_prime_UTR
AAGAAGCTCAAAATATTATG+TGG 0.525327 8_2:+42595787 Msa1248540:five_prime_UTR
CTAGGTTGATGCCTAACAAA+GGG 0.525508 8_2:+42597495 Msa1248540:CDS
GACAAATGTCAATGAAAATG+AGG 0.525546 8_2:+42595890 Msa1248540:five_prime_UTR
GTGGCGCCGCATAGAGGTTG+AGG 0.528015 8_2:+42595242 Msa1248540:five_prime_UTR
ATTGAGTTAATGCTGCTGAT+GGG 0.530195 8_2:+42597746 Msa1248540:CDS
GGAAACATGCTTCAGACCAT+TGG 0.541782 8_2:+42595911 Msa1248540:five_prime_UTR
GATTTATGAGCACATGCCTA+GGG 0.541821 8_2:+42595647 Msa1248540:five_prime_UTR
ACTTGATATTGGGGCTGAGA+GGG 0.547189 8_2:+42595815 Msa1248540:five_prime_UTR
GTGGAAATTACTTGATATTG+GGG 0.556011 8_2:+42595806 Msa1248540:five_prime_UTR
TCACTAATATTTATAACAAG+AGG 0.557518 8_2:-42595294 None:intergenic
GATTGAGTTAATGCTGCTGA+TGG 0.559562 8_2:+42597745 Msa1248540:CDS
CAATTCAGATTTCAGACCAA+TGG 0.560880 8_2:-42595927 None:intergenic
GGTATAACTGTTGCAGGAGA+CGG 0.562251 8_2:-42597473 None:intergenic
AGCATTGCGATAATGAATAC+CGG 0.565238 8_2:-42595718 None:intergenic
GAGGCAGTTAATTCGTCTAT+GGG 0.565783 8_2:+42595969 Msa1248540:exon
TGGTTTGAGAGATGAAATGA+TGG 0.567404 8_2:+42597823 Msa1248540:CDS
GAGAGGGTGCGAAAAGGTGT+TGG 0.567841 8_2:+42595831 Msa1248540:five_prime_UTR
GGGGCTGAGAGGGTGCGAAA+AGG 0.580980 8_2:+42595825 Msa1248540:five_prime_UTR
TACTTGATATTGGGGCTGAG+AGG 0.584455 8_2:+42595814 Msa1248540:five_prime_UTR
GAGTATCTTGAAGGAACAGG+TGG 0.588038 8_2:+42595375 Msa1248540:five_prime_UTR
GGTGCGAAAAGGTGTTGGCA+AGG 0.590379 8_2:+42595836 Msa1248540:five_prime_UTR
AAATTCAGATGTAGAAACAA+TGG 0.596835 8_2:+42595866 Msa1248540:five_prime_UTR
TGAGAGTACAAACAATGTGT+TGG 0.597259 8_2:+42595152 Msa1248540:five_prime_UTR
CTAATATTTATAACAAGAGG+AGG 0.599669 8_2:-42595291 None:intergenic
ATCATTGATCACTGAAATGG+AGG 0.600594 8_2:-42595688 None:intergenic
GAATCTTCTCCCATACCCCT+AGG 0.605803 8_2:-42595663 None:intergenic
TGCCCATTGAATCAAACTTG+CGG 0.606774 8_2:-42595524 None:intergenic
CCTAGGTTGATGCCTAACAA+AGG 0.608082 8_2:+42597494 Msa1248540:CDS
GGACAGTAGCTGATGCACAC+TGG 0.613720 8_2:+42597768 Msa1248540:CDS
TTGAGTTAATGCTGCTGATG+GGG 0.623949 8_2:+42597747 Msa1248540:CDS
GTGATCAATGATGATGATGC+CGG 0.629234 8_2:+42595699 Msa1248540:five_prime_UTR
AGGCATGTGCTCATAAATCA+CGG 0.631426 8_2:-42595643 None:intergenic
CCTTTGTTAGGCATCAACCT+AGG 0.631816 8_2:-42597494 None:intergenic
CTGGTATCAAGTCCTGCCAA+AGG 0.634306 8_2:+42595127 Msa1248540:five_prime_UTR
ATAAATTACATGGATCAACA+TGG 0.663527 8_2:-42597430 None:intergenic
CTCCTGCAACAGTTATACCT+AGG 0.666500 8_2:+42597477 Msa1248540:CDS
GCGCCGCATAGAGGTTGAGG+AGG 0.668856 8_2:+42595245 Msa1248540:five_prime_UTR
AGCATTAACTCAATCAGACA+CGG 0.698370 8_2:-42597737 None:intergenic
ATTTATGAGCACATGCCTAG+GGG 0.718468 8_2:+42595648 Msa1248540:five_prime_UTR
ATTCCTCCTCAACCTCTATG+CGG 0.727553 8_2:-42595248 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATATTAGTGAAACATTA+CGG + chr8_2:42595304-42595323 Msa1248540:five_prime_UTR 15.0%
!!! TGATATATTGTAAGTTTTAT+TGG + chr8_2:42596070-42596089 Msa1248540:intron 15.0%
!!! ATAATCTTTTTGGAAATATT+TGG + chr8_2:42596819-42596838 Msa1248540:intron 15.0%
!! TGAAATTTGAAGAAAAATTA+TGG - chr8_2:42597342-42597361 None:intergenic 15.0%
!! TCACTAATATTTATAACAAG+AGG - chr8_2:42595297-42595316 None:intergenic 20.0%
!!! CATATTTTTTATTAGGCTTA+TGG - chr8_2:42595461-42595480 None:intergenic 20.0%
!! TAAGCCTAATAAAAAATATG+TGG + chr8_2:42595461-42595480 Msa1248540:five_prime_UTR 20.0%
!! ACTACAATTAATCTTCATAT+TGG - chr8_2:42595502-42595521 None:intergenic 20.0%
!! TTCTACATCTGAATTTAAAT+TGG - chr8_2:42595862-42595881 None:intergenic 20.0%
!! AAAATAAGCATAAACAACTT+AGG - chr8_2:42596032-42596051 None:intergenic 20.0%
!!! TACTTTTTTTATCCTTTGAT+AGG + chr8_2:42596129-42596148 Msa1248540:intron 20.0%
!! TTAAGTCACTATATAGATAA+TGG + chr8_2:42596227-42596246 Msa1248540:intron 20.0%
!! AATGAAAAATGATAATTCCA+AGG - chr8_2:42596633-42596652 None:intergenic 20.0%
!!! TTTGAAATTCACCTTTTTAA+GGG + chr8_2:42596906-42596925 Msa1248540:intron 20.0%
!! AAATTCCAATAATTGAGAAA+GGG - chr8_2:42597301-42597320 None:intergenic 20.0%
!! AAAATTCCAATAATTGAGAA+AGG - chr8_2:42597302-42597321 None:intergenic 20.0%
!!! TGATTTTAATAGTAGTTTGA+GGG + chr8_2:42597616-42597635 Msa1248540:CDS 20.0%
! CTAATATTTATAACAAGAGG+AGG - chr8_2:42595294-42595313 None:intergenic 25.0%
! TTTCTAAATGAGTATCTTGA+AGG + chr8_2:42595366-42595385 Msa1248540:five_prime_UTR 25.0%
!!! GTCACCACATATTTTTTATT+AGG - chr8_2:42595468-42595487 None:intergenic 25.0%
! TTGCGGATTTATAACATTAA+AGG + chr8_2:42595564-42595583 Msa1248540:five_prime_UTR 25.0%
! ACCAATTCTTCTTAAAAAGA+TGG + chr8_2:42595604-42595623 Msa1248540:five_prime_UTR 25.0%
!!! ACCATCTTTTTAAGAAGAAT+TGG - chr8_2:42595608-42595627 None:intergenic 25.0%
! AAGAAGCTCAAAATATTATG+TGG + chr8_2:42595787-42595806 Msa1248540:five_prime_UTR 25.0%
! ATGTGGAAATTACTTGATAT+TGG + chr8_2:42595804-42595823 Msa1248540:five_prime_UTR 25.0%
! TGTGGAAATTACTTGATATT+GGG + chr8_2:42595805-42595824 Msa1248540:five_prime_UTR 25.0%
! AAATTCAGATGTAGAAACAA+TGG + chr8_2:42595866-42595885 Msa1248540:five_prime_UTR 25.0%
!!! TTGATATATTTTTGCAACAG+AGG + chr8_2:42595947-42595966 Msa1248540:five_prime_UTR 25.0%
! ACTTACAATATATCAACATG+CGG - chr8_2:42596065-42596084 None:intergenic 25.0%
! GTTATTGAATAGAATAGCAA+TGG - chr8_2:42596177-42596196 None:intergenic 25.0%
! AGAGATCTTAAAATTACTGT+TGG + chr8_2:42596297-42596316 Msa1248540:intron 25.0%
!! GGAAGTTTGAAATTATATGA+TGG + chr8_2:42596373-42596392 Msa1248540:intron 25.0%
!! TTGTTCTATATTTGTTGCTT+AGG + chr8_2:42596409-42596428 Msa1248540:intron 25.0%
! TTTAACAAGAAAAGCTCAAA+TGG + chr8_2:42596599-42596618 Msa1248540:intron 25.0%
!! TCATAATGGCATAATCTTTT+TGG + chr8_2:42596809-42596828 Msa1248540:intron 25.0%
!!! GTTTGAAATTCACCTTTTTA+AGG + chr8_2:42596905-42596924 Msa1248540:intron 25.0%
!!! TTGAAATTCACCTTTTTAAG+GGG + chr8_2:42596907-42596926 Msa1248540:intron 25.0%
!!! TTAATGTGAAATTTTGACCT+TGG + chr8_2:42597070-42597089 Msa1248540:intron 25.0%
! ATACTAAAATTCAAATGCCA+AGG - chr8_2:42597090-42597109 None:intergenic 25.0%
!!! TTGGCATTTGAATTTTAGTA+TGG + chr8_2:42597089-42597108 Msa1248540:intron 25.0%
! AAAGAATATTAAGTCGGAAT+GGG + chr8_2:42597144-42597163 Msa1248540:intron 25.0%
! CATTATACATTCATCATCTA+AGG + chr8_2:42597169-42597188 Msa1248540:intron 25.0%
!! TCAAATTTCAAATCGTGTTT+TGG + chr8_2:42597352-42597371 Msa1248540:intron 25.0%
! AAAATTGTTGAAGAGAATTC+AGG + chr8_2:42597401-42597420 Msa1248540:intron 25.0%
! ATAAATTACATGGATCAACA+TGG - chr8_2:42597433-42597452 None:intergenic 25.0%
! TGATCCATGTAATTTATCTT+TGG + chr8_2:42597436-42597455 Msa1248540:CDS 25.0%
! TTAGCCAAAGATAAATTACA+TGG - chr8_2:42597443-42597462 None:intergenic 25.0%
! CACAATATGAAAAACAATCA+AGG - chr8_2:42597546-42597565 None:intergenic 25.0%
!!! GTGATTTTAATAGTAGTTTG+AGG + chr8_2:42597615-42597634 Msa1248540:CDS 25.0%
!! AGGTTAAATATCAAAGTATC+TGG - chr8_2:42597674-42597693 None:intergenic 25.0%
!! TGTGATAATTTTGCAGATTT+CGG + chr8_2:42597793-42597812 Msa1248540:CDS 25.0%
! GAATTGAAATGTCATATTGA+TGG + chr8_2:42597845-42597864 Msa1248540:CDS 25.0%
TTGAGAAGATGTTGAAAAAG+TGG + chr8_2:42595223-42595242 Msa1248540:five_prime_UTR 30.0%
!! CTTTTTAAGAAGAATTGGTC+AGG - chr8_2:42595603-42595622 None:intergenic 30.0%
AAAGATGGTCAAAAAATGCA+AGG + chr8_2:42595619-42595638 Msa1248540:five_prime_UTR 30.0%
ATCATCATTGATCACTGAAA+TGG - chr8_2:42595694-42595713 None:intergenic 30.0%
GTGGAAATTACTTGATATTG+GGG + chr8_2:42595806-42595825 Msa1248540:five_prime_UTR 30.0%
GACAAATGTCAATGAAAATG+AGG + chr8_2:42595890-42595909 Msa1248540:five_prime_UTR 30.0%
!! ATATATTTTTGCAACAGAGG+AGG + chr8_2:42595950-42595969 Msa1248540:five_prime_UTR 30.0%
ATAAGCATAAACAACTTAGG+AGG - chr8_2:42596029-42596048 None:intergenic 30.0%
AAGGATGATATTCCTATCAA+AGG - chr8_2:42596144-42596163 None:intergenic 30.0%
ATTGAATAGAATAGCAATGG+TGG - chr8_2:42596174-42596193 None:intergenic 30.0%
ACAAAACTCTGAACATCTAT+TGG - chr8_2:42596509-42596528 None:intergenic 30.0%
AATTCTGTCATTGACACAAT+GGG - chr8_2:42596566-42596585 None:intergenic 30.0%
AAATTCTGTCATTGACACAA+TGG - chr8_2:42596567-42596586 None:intergenic 30.0%
AGTATGTAATGATAACTTCG+AGG - chr8_2:42596692-42596711 None:intergenic 30.0%
ACATTGCATATTATATGCAG+AGG - chr8_2:42596965-42596984 None:intergenic 30.0%
CTAGCCAAAGAATATTAAGT+CGG + chr8_2:42597138-42597157 Msa1248540:intron 30.0%
CATTCCGACTTAATATTCTT+TGG - chr8_2:42597145-42597164 None:intergenic 30.0%
CAAAGAATATTAAGTCGGAA+TGG + chr8_2:42597143-42597162 Msa1248540:intron 30.0%
AAATTATGGTTCACCTAACT+TGG - chr8_2:42597328-42597347 None:intergenic 30.0%
CATATTGTGAAGACAGTAAT+TGG + chr8_2:42597557-42597576 Msa1248540:CDS 30.0%
TAGTAGTTTGAGGGATAAAA+AGG + chr8_2:42597625-42597644 Msa1248540:CDS 30.0%
TGTTTGTACTCTCAATCCTT+TGG - chr8_2:42595146-42595165 None:intergenic 35.0%
! TGAGAGTACAAACAATGTGT+TGG + chr8_2:42595152-42595171 Msa1248540:five_prime_UTR 35.0%
AACTCTCACACAATTCTTAG+TGG + chr8_2:42595197-42595216 Msa1248540:five_prime_UTR 35.0%
AATGAGTATCTTGAAGGAAC+AGG + chr8_2:42595372-42595391 Msa1248540:five_prime_UTR 35.0%
TTCTTTGTAGTCATGCTTTG+CGG + chr8_2:42595547-42595566 Msa1248540:five_prime_UTR 35.0%
ATCATTGATCACTGAAATGG+AGG - chr8_2:42595691-42595710 None:intergenic 35.0%
AGCATTGCGATAATGAATAC+CGG - chr8_2:42595721-42595740 None:intergenic 35.0%
! GTCATTCTACCATCTAGTTT+CGG + chr8_2:42595749-42595768 Msa1248540:five_prime_UTR 35.0%
CAATTCAGATTTCAGACCAA+TGG - chr8_2:42595930-42595949 None:intergenic 35.0%
! TAGCAATGGTGGAGTAATTA+AGG - chr8_2:42596163-42596182 None:intergenic 35.0%
AAAGTAGCAATTCCCGTAAA+GGG + chr8_2:42596327-42596346 Msa1248540:intron 35.0%
GAATCTGTTAAACCTTCACA+AGG + chr8_2:42596483-42596502 Msa1248540:intron 35.0%
AGAAAAGCTCAAATGGCATT+TGG + chr8_2:42596606-42596625 Msa1248540:intron 35.0%
CTTTAGCATGTCTTTGCATA+TGG + chr8_2:42596665-42596684 Msa1248540:intron 35.0%
TATAGGATATATTGCTCCAG+AGG + chr8_2:42596758-42596777 Msa1248540:intron 35.0%
! TTTGGTTGGCTGTTTCATAA+TGG + chr8_2:42596795-42596814 Msa1248540:intron 35.0%
AAGGTGAATTTCAAACCACT+GGG - chr8_2:42596901-42596920 None:intergenic 35.0%
AAAGGTGAATTTCAAACCAC+TGG - chr8_2:42596902-42596921 None:intergenic 35.0%
CTCACTTATTCCCCTTAAAA+AGG - chr8_2:42596920-42596939 None:intergenic 35.0%
!! CTTTTTAAGGGGAATAAGTG+AGG + chr8_2:42596918-42596937 Msa1248540:intron 35.0%
! TCATCATCTAAGGTAGTTGT+TGG + chr8_2:42597179-42597198 Msa1248540:intron 35.0%
AAATCCACTAAGCTGATCTT+AGG + chr8_2:42597225-42597244 Msa1248540:intron 35.0%
CTTTGCCCTTTCTCAATTAT+TGG + chr8_2:42597293-42597312 Msa1248540:intron 35.0%
! TTGGAATTTTCTGCCAAGTT+AGG + chr8_2:42597312-42597331 Msa1248540:intron 35.0%
AAGGACAAAAATTGCACAGA+TGG - chr8_2:42597527-42597546 None:intergenic 35.0%
TCACACAAGCATCAAGAAAA+AGG - chr8_2:42597694-42597713 None:intergenic 35.0%
! TTTTTCTTGATGCTTGTGTG+AGG + chr8_2:42597693-42597712 Msa1248540:CDS 35.0%
AGCATTAACTCAATCAGACA+CGG - chr8_2:42597740-42597759 None:intergenic 35.0%
ATTGAGTTAATGCTGCTGAT+GGG + chr8_2:42597746-42597765 Msa1248540:CDS 35.0%
TTGCAGATTTCGGATATGAA+TGG + chr8_2:42597803-42597822 Msa1248540:CDS 35.0%
TGGTTTGAGAGATGAAATGA+TGG + chr8_2:42597823-42597842 Msa1248540:CDS 35.0%
! TAACAAGAGGAGGTGTACTT+TGG - chr8_2:42595284-42595303 None:intergenic 40.0%
AACATTACGGCATGCATCTA+CGG + chr8_2:42595317-42595336 Msa1248540:five_prime_UTR 40.0%
TTGCCGCAAGTTTGATTCAA+TGG + chr8_2:42595521-42595540 Msa1248540:five_prime_UTR 40.0%
TGCCGCAAGTTTGATTCAAT+GGG + chr8_2:42595522-42595541 Msa1248540:five_prime_UTR 40.0%
TGCCCATTGAATCAAACTTG+CGG - chr8_2:42595527-42595546 None:intergenic 40.0%
AGGCATGTGCTCATAAATCA+CGG - chr8_2:42595646-42595665 None:intergenic 40.0%
TGATTTATGAGCACATGCCT+AGG + chr8_2:42595646-42595665 Msa1248540:five_prime_UTR 40.0%
GATTTATGAGCACATGCCTA+GGG + chr8_2:42595647-42595666 Msa1248540:five_prime_UTR 40.0%
ATTTATGAGCACATGCCTAG+GGG + chr8_2:42595648-42595667 Msa1248540:five_prime_UTR 40.0%
! GTGATCAATGATGATGATGC+CGG + chr8_2:42595699-42595718 Msa1248540:five_prime_UTR 40.0%
CTTGACTTTCCGAAACTAGA+TGG - chr8_2:42595761-42595780 None:intergenic 40.0%
GAGGCAGTTAATTCGTCTAT+GGG + chr8_2:42595969-42595988 Msa1248540:exon 40.0%
GTTCGTGCAATTGAGAACAT+TGG - chr8_2:42596003-42596022 None:intergenic 40.0%
GAAAGTAGCAATTCCCGTAA+AGG + chr8_2:42596326-42596345 Msa1248540:intron 40.0%
ATGCCAATGTGTTCATAGGA+TGG + chr8_2:42596352-42596371 Msa1248540:intron 40.0%
CTTCCATCCTATGAACACAT+TGG - chr8_2:42596358-42596377 None:intergenic 40.0%
!! AACATCTATTGGCCTTGTGA+AGG - chr8_2:42596498-42596517 None:intergenic 40.0%
! CTTCTCAGCAATGGTGTTTT+GGG - chr8_2:42596733-42596752 None:intergenic 40.0%
! TCTTCTCAGCAATGGTGTTT+TGG - chr8_2:42596734-42596753 None:intergenic 40.0%
AAAACACCATTGCTGAGAAG+AGG + chr8_2:42596733-42596752 Msa1248540:intron 40.0%
AAACACCATTGCTGAGAAGA+GGG + chr8_2:42596734-42596753 Msa1248540:intron 40.0%
!! TGTTCCGCAGAAATTTTGGT+TGG + chr8_2:42596781-42596800 Msa1248540:intron 40.0%
ACAGCCAACCAAAATTTCTG+CGG - chr8_2:42596788-42596807 None:intergenic 40.0%
! TTTTTCGTCAAGTAACCCAG+TGG + chr8_2:42596883-42596902 Msa1248540:intron 40.0%
TGCAATGTGAGTTAAGCTCA+CGG + chr8_2:42596977-42596996 Msa1248540:intron 40.0%
AACCTAGGTATAACTGTTGC+AGG - chr8_2:42597482-42597501 None:intergenic 40.0%
!! CTAGGTTGATGCCTAACAAA+GGG + chr8_2:42597495-42597514 Msa1248540:CDS 40.0%
GATTGAGTTAATGCTGCTGA+TGG + chr8_2:42597745-42597764 Msa1248540:CDS 40.0%
TTGAGTTAATGCTGCTGATG+GGG + chr8_2:42597747-42597766 Msa1248540:CDS 40.0%
TGTACTCTCAATCCTTTGGC+AGG - chr8_2:42595142-42595161 None:intergenic 45.0%
ATTCCTCCTCAACCTCTATG+CGG - chr8_2:42595251-42595270 None:intergenic 45.0%
GAGTATCTTGAAGGAACAGG+TGG + chr8_2:42595375-42595394 Msa1248540:five_prime_UTR 45.0%
TACTTGATATTGGGGCTGAG+AGG + chr8_2:42595814-42595833 Msa1248540:five_prime_UTR 45.0%
ACTTGATATTGGGGCTGAGA+GGG + chr8_2:42595815-42595834 Msa1248540:five_prime_UTR 45.0%
GGAAACATGCTTCAGACCAT+TGG + chr8_2:42595911-42595930 Msa1248540:five_prime_UTR 45.0%
GGAGGCAGTTAATTCGTCTA+TGG + chr8_2:42595968-42595987 Msa1248540:exon 45.0%
ACATTGGCATTGCCCTTTAC+GGG - chr8_2:42596342-42596361 None:intergenic 45.0%
CACATTGGCATTGCCCTTTA+CGG - chr8_2:42596343-42596362 None:intergenic 45.0%
GGCAATGCCAATGTGTTCAT+AGG + chr8_2:42596348-42596367 Msa1248540:intron 45.0%
CTCAAATGGCATTTGGACCT+TGG + chr8_2:42596613-42596632 Msa1248540:intron 45.0%
TATAGCCCTCTTCTCAGCAA+TGG - chr8_2:42596742-42596761 None:intergenic 45.0%
ATTGCTGAGAAGAGGGCTAT+AGG + chr8_2:42596741-42596760 Msa1248540:intron 45.0%
AAATTTCTGCGGAACACCTC+TGG - chr8_2:42596777-42596796 None:intergenic 45.0%
!! GAGGTGTTCCGCAGAAATTT+TGG + chr8_2:42596777-42596796 Msa1248540:intron 45.0%
AGGGGAATAAGTGAGGTGTA+TGG + chr8_2:42596925-42596944 Msa1248540:intron 45.0%
GGGGAATAAGTGAGGTGTAT+GGG + chr8_2:42596926-42596945 Msa1248540:intron 45.0%
GGGAATAAGTGAGGTGTATG+GGG + chr8_2:42596927-42596946 Msa1248540:intron 45.0%
GCAATGTGAGTTAAGCTCAC+GGG + chr8_2:42596978-42596997 Msa1248540:intron 45.0%
GCTTCCTAAGATCAGCTTAG+TGG - chr8_2:42597232-42597251 None:intergenic 45.0%
GTTGCAGCAGCATACATTAC+TGG + chr8_2:42597256-42597275 Msa1248540:intron 45.0%
GGTATAACTGTTGCAGGAGA+CGG - chr8_2:42597476-42597495 None:intergenic 45.0%
CTCCTGCAACAGTTATACCT+AGG + chr8_2:42597477-42597496 Msa1248540:CDS 45.0%
CCTTTGTTAGGCATCAACCT+AGG - chr8_2:42597497-42597516 None:intergenic 45.0%
!! CCTAGGTTGATGCCTAACAA+AGG + chr8_2:42597494-42597513 Msa1248540:CDS 45.0%
GATGGCACAAACCCTTTGTT+AGG - chr8_2:42597509-42597528 None:intergenic 45.0%
CTGGTATCAAGTCCTGCCAA+AGG + chr8_2:42595127-42595146 Msa1248540:five_prime_UTR 50.0%
GAATCTTCTCCCATACCCCT+AGG - chr8_2:42595666-42595685 None:intergenic 50.0%
TGAGTTAAGCTCACGGGTCA+CGG + chr8_2:42596984-42597003 Msa1248540:intron 50.0%
GAAAAAGTGGCGCCGCATAG+AGG + chr8_2:42595236-42595255 Msa1248540:five_prime_UTR 55.0%
TGAGCACATGCCTAGGGGTA+TGG + chr8_2:42595653-42595672 Msa1248540:five_prime_UTR 55.0%
! GAGCACATGCCTAGGGGTAT+GGG + chr8_2:42595654-42595673 Msa1248540:five_prime_UTR 55.0%
! GAGAGGGTGCGAAAAGGTGT+TGG + chr8_2:42595831-42595850 Msa1248540:five_prime_UTR 55.0%
!! GGTGCGAAAAGGTGTTGGCA+AGG + chr8_2:42595836-42595855 Msa1248540:five_prime_UTR 55.0%
GGTGTATGGGGTTCGAACGT+CGG + chr8_2:42596939-42596958 Msa1248540:intron 55.0%
GGACAGTAGCTGATGCACAC+TGG + chr8_2:42597768-42597787 Msa1248540:CDS 55.0%
GTTAAGCTCACGGGTCACGG+CGG + chr8_2:42596987-42597006 Msa1248540:intron 60.0%
GTGGCGCCGCATAGAGGTTG+AGG + chr8_2:42595242-42595261 Msa1248540:five_prime_UTR 65.0%
GCGCCGCATAGAGGTTGAGG+AGG + chr8_2:42595245-42595264 Msa1248540:five_prime_UTR 65.0%
! GGGGCTGAGAGGGTGCGAAA+AGG + chr8_2:42595825-42595844 Msa1248540:five_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 42595123 42597934 42595123 ID=Msa1248540;Name=Msa1248540
chr8_2 mRNA 42595123 42597934 42595123 ID=Msa1248540-mRNA-1;Parent=Msa1248540;Name=Msa1248540-mRNA-1;_AED=0.50;_eAED=0.50;_QI=864|0|0.5|0.5|1|1|2|0|171
chr8_2 exon 42595123 42595990 42595123 ID=Msa1248540-mRNA-1:exon:12955;Parent=Msa1248540-mRNA-1
chr8_2 exon 42597423 42597934 42597423 ID=Msa1248540-mRNA-1:exon:12956;Parent=Msa1248540-mRNA-1
chr8_2 five_prime_UTR 42595123 42595986 42595123 ID=Msa1248540-mRNA-1:five_prime_utr;Parent=Msa1248540-mRNA-1
chr8_2 CDS 42595987 42595990 42595987 ID=Msa1248540-mRNA-1:cds;Parent=Msa1248540-mRNA-1
chr8_2 CDS 42597423 42597934 42597423 ID=Msa1248540-mRNA-1:cds;Parent=Msa1248540-mRNA-1
Gene Sequence

>Msa1248540

ATGGGTTTGATCCATGTTGATCCATGTAATTTATCTTTGGCTAAAATGCAAATGCCGTCTCCTGCAACAGTTATACCTAGGTTGATGCCTAACAAAGGGTTTGTGCCATCTGTGCAATTTTTGTCCTTGATTGTTTTTCATATTGTGAAGACAGTAATTGGAACATGTCTTGTATCTAATTATTTGTCTCGAGTTTGTGATTTTAATAGTAGTTTGAGGGATAAAAAGGCGCAACATGCAATTGTGTTGACGCCAGATACTTTGATATTTAACCTTTTTCTTGATGCTTGTGTGAGGCTTAAGTTGTGTTTGAAAGTACCGTGTCTGATTGAGTTAATGCTGCTGATGGGGACAGTAGCTGATGCACACTGGATTGTGATAATTTTGCAGATTTCGGATATGAATGGTTTGAGAGATGAAATGATGGAATTGAAATGTCATATTGATGGAGTTTCAACTGCTTATGTTAAGCATTATCGTCAATTTAATGATAGTTTGTTGAGCTTGCACTTTTAA
Protein sequence

>Msa1248540

MGLIHVDPCNLSLAKMQMPSPATVIPRLMPNKGFVPSVQFLSLIVFHIVKTVIGTCLVSNYLSRVCDFNSSLRDKKAQHAIVLTPDTLIFNLFLDACVRLKLCLKVPCLIELMLLMGTVADAHWIVIILQISDMNGLRDEMMELKCHIDGVSTAYVKHYRQFNDSLLSLHF*