AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1248960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1248960 MtrunA17_Chr7g0227381 63.218 87 5 3 1 64 280 362 1.10e-20 82.8
Msa1248960 MtrunA17_Chr8g0364491 59.524 84 13 3 1 65 275 356 1.05e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 65 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCACCTTGAAGAGTAATT+TGG 0.164526 8_2:+43328738 Msa1248960:five_prime_UTR
TGATTATCCGGACGAGATTT+CGG 0.287103 8_2:+43331029 Msa1248960:CDS
TGAGGGCTGAAGAGAGAAAA+AGG 0.293209 8_2:+43328600 Msa1248960:five_prime_UTR
GATGAAGAAGATTATTTGTA+TGG 0.320758 8_2:+43330866 Msa1248960:five_prime_UTR
AATCAAAATGATTGTGGGTA+TGG 0.340235 8_2:-43328492 None:intergenic
AATTCCCAAATTACTCTTCA+AGG 0.343203 8_2:-43328743 None:intergenic
TCAACCTCTGCAGCGGCATT+AGG 0.347281 8_2:-43330379 None:intergenic
TTCACCTTGAAGAGTAATTT+GGG 0.360202 8_2:+43328739 Msa1248960:five_prime_UTR
ATCCACCATTGGAGAGATTA+GGG 0.361540 8_2:+43328716 Msa1248960:five_prime_UTR
CTTCTCTCAAGAAACAAATA+AGG 0.370343 8_2:+43328776 Msa1248960:five_prime_UTR
TTCACTTCTTCAATAAATCA+AGG 0.409052 8_2:+43328559 Msa1248960:five_prime_UTR
AATCCACCATTGGAGAGATT+AGG 0.423711 8_2:+43328715 Msa1248960:five_prime_UTR
AAATCCAATGAATGATTATC+CGG 0.442829 8_2:+43331017 Msa1248960:exon
CCATGGTTTATACCTCATCT+TGG 0.452908 8_2:-43328652 None:intergenic
GGAATAACCTCTCTTAGTAA+AGG 0.453029 8_2:-43330358 None:intergenic
TACAATAATCAAAATGATTG+TGG 0.455475 8_2:-43328498 None:intergenic
ACAATAATCAAAATGATTGT+GGG 0.461891 8_2:-43328497 None:intergenic
GAATAACCTCTCTTAGTAAA+GGG 0.463078 8_2:-43330357 None:intergenic
CTCATCTTGTTCTCACTACC+TGG 0.463111 8_2:+43328455 Msa1248960:five_prime_UTR
GTTTCATAATCTGAATTGTC+AGG 0.463337 8_2:-43330890 None:intergenic
ATCAAAATGATTGTGGGTAT+GGG 0.479465 8_2:-43328491 None:intergenic
AATTAGTGAGAGAGATGATG+AGG 0.481100 8_2:+43328582 Msa1248960:five_prime_UTR
TCGTCCGGATAATCATTCAT+TGG 0.491166 8_2:-43331021 None:intergenic
TGTGGGTATGGGGTGAAGGA+TGG 0.492753 8_2:-43328480 None:intergenic
TGATTGTGGGTATGGGGTGA+AGG 0.503876 8_2:-43328484 None:intergenic
TCATCTTCCGAAATCTCGTC+CGG 0.506670 8_2:-43331036 None:intergenic
CAATGATGAGGATGAGGATG+AGG 0.511269 8_2:+43331237 Msa1248960:CDS
TATGAAACTGACGACTCAAA+TGG 0.514225 8_2:+43330905 Msa1248960:five_prime_UTR
GGTTTATACCTCATCTTGGT+CGG 0.519331 8_2:-43328648 None:intergenic
TTCTGATCCATTGTATGATG+AGG 0.519454 8_2:+43331177 Msa1248960:CDS
TTTCCTCCCTTTACTAAGAG+AGG 0.528946 8_2:+43330351 Msa1248960:five_prime_UTR
ATGAAGAAGATTATTTGTAT+GGG 0.529205 8_2:+43330867 Msa1248960:five_prime_UTR
AAATTTCAGCTCCTCTCCCA+TGG 0.529808 8_2:-43328669 None:intergenic
GAGATTTCGGAAGATGAAGA+AGG 0.532402 8_2:+43331042 Msa1248960:CDS
TTTGATGATTATGAAGGTCA+AGG 0.533767 8_2:+43331202 Msa1248960:CDS
ATGCAGAGATATCTCCTTGG+AGG 0.535146 8_2:+43330309 Msa1248960:intron
ATGTGGGTAATGAGCATTGA+AGG 0.543289 8_2:+43331266 Msa1248960:CDS
ATGGGGTGAAGGATGGTACC+AGG 0.551102 8_2:-43328473 None:intergenic
ATTAGTGAGAGAGATGATGA+GGG 0.556768 8_2:+43328583 Msa1248960:five_prime_UTR
CAAAAGTCTTTGATCCTCCA+AGG 0.567079 8_2:-43330323 None:intergenic
GTGAACCCTAATCTCTCCAA+TGG 0.570926 8_2:-43328721 None:intergenic
GATTATGAAGGTCAAGGTGT+AGG 0.571993 8_2:+43331208 Msa1248960:CDS
TGAGGATGAGGATGAAGATG+TGG 0.584840 8_2:+43331249 Msa1248960:CDS
AACCCTAATCTCTCCAATGG+TGG 0.586333 8_2:-43328718 None:intergenic
CTATGACAATGATGAGGATG+AGG 0.587104 8_2:+43331231 Msa1248960:CDS
TCAAAATCCTCATCATACAA+TGG 0.590172 8_2:-43331184 None:intergenic
TATTCCTAATGCCGCTGCAG+AGG 0.592055 8_2:+43330375 Msa1248960:five_prime_UTR
TGATTGAGAGGTAGAAAGAG+AGG 0.600610 8_2:+43328625 Msa1248960:five_prime_UTR
AATAGATGAGAATCCACCAT+TGG 0.601690 8_2:+43328705 Msa1248960:five_prime_UTR
TTATGACCTCTACACTGTAA+CGG 0.604149 8_2:+43330711 Msa1248960:five_prime_UTR
CCAAGATGAGGTATAAACCA+TGG 0.609551 8_2:+43328652 Msa1248960:five_prime_UTR
TGAGGTATAAACCATGGGAG+AGG 0.612118 8_2:+43328658 Msa1248960:five_prime_UTR
CAAGATGAGGTATAAACCAT+GGG 0.615549 8_2:+43328653 Msa1248960:five_prime_UTR
TCAAAATGATTGTGGGTATG+GGG 0.616761 8_2:-43328490 None:intergenic
AAGAGAGGCCGACCAAGATG+AGG 0.618165 8_2:+43328640 Msa1248960:five_prime_UTR
AGGTAGTGAGAACAAGATGA+GGG 0.624482 8_2:-43328453 None:intergenic
ATAAGTGCTCATTCCAAACA+AGG 0.626145 8_2:+43330409 Msa1248960:five_prime_UTR
TAACCTCTCTTAGTAAAGGG+AGG 0.626192 8_2:-43330354 None:intergenic
GAGGATGAGGATGAAGATGT+GGG 0.629754 8_2:+43331250 Msa1248960:CDS
TTAATGCAGAGATATCTCCT+TGG 0.630700 8_2:+43330306 Msa1248960:intron
ATCAGCTTCAACCTCTGCAG+CGG 0.637554 8_2:-43330386 None:intergenic
CAGGTAGTGAGAACAAGATG+AGG 0.637789 8_2:-43328454 None:intergenic
TCTCATCCGTTACAGTGTAG+AGG 0.640074 8_2:-43330717 None:intergenic
TGTAGGCTATGACAATGATG+AGG 0.727984 8_2:+43331225 Msa1248960:CDS
TCAGAAGAAAGCAAGCATAG+CGG 0.746054 8_2:+43331078 Msa1248960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATGTTAACTTAATAA+TGG + chr8_2:43328937-43328956 Msa1248960:intron 10.0%
!! AATATCTTCAATAATAAATA+AGG + chr8_2:43330165-43330184 Msa1248960:intron 10.0%
!! TAAAGTAGATTATTATCTAA+AGG + chr8_2:43329186-43329205 Msa1248960:intron 15.0%
!! CATATAAATCCAAAAAAATT+AGG - chr8_2:43330081-43330100 None:intergenic 15.0%
!!! CTAATTTTTTTGGATTTATA+TGG + chr8_2:43330079-43330098 Msa1248960:intron 15.0%
!!! TAAATAATTGATGTATTTTC+TGG + chr8_2:43330511-43330530 Msa1248960:intron 15.0%
!!! AAATAATTGATGTATTTTCT+GGG + chr8_2:43330512-43330531 Msa1248960:intron 15.0%
!! ACAATAATCAAAATGATTGT+GGG - chr8_2:43328500-43328519 None:intergenic 20.0%
!! TACAATAATCAAAATGATTG+TGG - chr8_2:43328501-43328520 None:intergenic 20.0%
!!! TTTTTGAAATAAGACATGAA+TGG + chr8_2:43329137-43329156 Msa1248960:intron 20.0%
!! TATTAGTAAGTGAGAATAAT+AGG + chr8_2:43329282-43329301 Msa1248960:intron 20.0%
!! GATTACAAGTCATAAATTTA+GGG - chr8_2:43329652-43329671 None:intergenic 20.0%
!!! TTATCTTGACCTAATTTTTT+TGG + chr8_2:43330069-43330088 Msa1248960:intron 20.0%
!!! ATTTTTTTGGATTTATATGG+AGG + chr8_2:43330082-43330101 Msa1248960:intron 20.0%
!! AAAAAAATAAGCGAAACTTA+AGG - chr8_2:43330488-43330507 None:intergenic 20.0%
!!! CAATAAAGACAAACATATTT+TGG + chr8_2:43330536-43330555 Msa1248960:intron 20.0%
!! TTTGTCTATACATGTTTAAT+CGG + chr8_2:43330785-43330804 Msa1248960:intron 20.0%
!! ATGAAGAAGATTATTTGTAT+GGG + chr8_2:43330867-43330886 Msa1248960:five_prime_UTR 20.0%
!! TTAAGAGTAAAGATAAGAAT+AGG - chr8_2:43330943-43330962 None:intergenic 20.0%
! TTCACTTCTTCAATAAATCA+AGG + chr8_2:43328559-43328578 Msa1248960:five_prime_UTR 25.0%
!!! ATCTTTTAGAGGTTTTAATG+GGG - chr8_2:43328813-43328832 None:intergenic 25.0%
!!! CATCTTTTAGAGGTTTTAAT+GGG - chr8_2:43328814-43328833 None:intergenic 25.0%
!!! ACATCTTTTAGAGGTTTTAA+TGG - chr8_2:43328815-43328834 None:intergenic 25.0%
!! ATAGATCAGTAAGAGTTTTA+AGG + chr8_2:43328914-43328933 Msa1248960:intron 25.0%
! TGTGTGAAATCATGATATTA+TGG + chr8_2:43328998-43329017 Msa1248960:intron 25.0%
! TTTCGACTTAAACTTTAGTT+TGG - chr8_2:43329555-43329574 None:intergenic 25.0%
! ACTAAAGTTTAAGTCGAAAT+TGG + chr8_2:43329556-43329575 Msa1248960:intron 25.0%
! CTAAAGTTTAAGTCGAAATT+GGG + chr8_2:43329557-43329576 Msa1248960:intron 25.0%
! CGATTACAAGTCATAAATTT+AGG - chr8_2:43329653-43329672 None:intergenic 25.0%
! AGCAGAATTCTTAAAATTGT+GGG - chr8_2:43329692-43329711 None:intergenic 25.0%
!!! GCATTTTAGTATTTTCAGTT+GGG - chr8_2:43329740-43329759 None:intergenic 25.0%
! AACTTAAATAACCCATTTGA+TGG + chr8_2:43329855-43329874 Msa1248960:intron 25.0%
! AGAATGTTTCGTATATACAA+GGG + chr8_2:43330033-43330052 Msa1248960:intron 25.0%
! TTGAACTATTGATGAAAAGA+GGG + chr8_2:43330114-43330133 Msa1248960:intron 25.0%
!! TTGTGTTGCTATATAAATGA+GGG + chr8_2:43330240-43330259 Msa1248960:intron 25.0%
! TTGTCTATACATGTTTAATC+GGG + chr8_2:43330786-43330805 Msa1248960:intron 25.0%
! GATGAAGAAGATTATTTGTA+TGG + chr8_2:43330866-43330885 Msa1248960:five_prime_UTR 25.0%
! AAATCCAATGAATGATTATC+CGG + chr8_2:43331017-43331036 Msa1248960:exon 25.0%
ATCAAAATGATTGTGGGTAT+GGG - chr8_2:43328494-43328513 None:intergenic 30.0%
AATCAAAATGATTGTGGGTA+TGG - chr8_2:43328495-43328514 None:intergenic 30.0%
! CTTTGCATGTTTTGTCATAT+TGG - chr8_2:43328532-43328551 None:intergenic 30.0%
TTCACCTTGAAGAGTAATTT+GGG + chr8_2:43328739-43328758 Msa1248960:five_prime_UTR 30.0%
AATTCCCAAATTACTCTTCA+AGG - chr8_2:43328746-43328765 None:intergenic 30.0%
CTTCTCTCAAGAAACAAATA+AGG + chr8_2:43328776-43328795 Msa1248960:five_prime_UTR 30.0%
! AATCTAAGCACATCTTTTAG+AGG - chr8_2:43328824-43328843 None:intergenic 30.0%
GAATCAATCACATGCAAATA+GGG - chr8_2:43328881-43328900 None:intergenic 30.0%
AGAATCAATCACATGCAAAT+AGG - chr8_2:43328882-43328901 None:intergenic 30.0%
! TAATTTTGCATGCCTTAATG+TGG + chr8_2:43329038-43329057 Msa1248960:intron 30.0%
ATGGAGTAGATGATTATTCA+AGG + chr8_2:43329156-43329175 Msa1248960:intron 30.0%
TGGAGTAGATGATTATTCAA+GGG + chr8_2:43329157-43329176 Msa1248960:intron 30.0%
TAATAGGATTCATCCTTGTA+AGG + chr8_2:43329298-43329317 Msa1248960:intron 30.0%
! TTAACCTCTTTTGGTAAGAT+TGG - chr8_2:43329400-43329419 None:intergenic 30.0%
!!! CTCATAGTTTTAACCTCTTT+TGG - chr8_2:43329409-43329428 None:intergenic 30.0%
CAAAAGAGGTTAAAACTATG+AGG + chr8_2:43329407-43329426 Msa1248960:intron 30.0%
ACAAGGAGGTTAAATTTAAG+AGG + chr8_2:43329495-43329514 Msa1248960:intron 30.0%
GCAGAATTCTTAAAATTGTG+GGG - chr8_2:43329691-43329710 None:intergenic 30.0%
GAGCAGAATTCTTAAAATTG+TGG - chr8_2:43329693-43329712 None:intergenic 30.0%
!! GGCATTTTAGTATTTTCAGT+TGG - chr8_2:43329741-43329760 None:intergenic 30.0%
!!! ATGATCATCATCACTTTTTG+AGG + chr8_2:43329831-43329850 Msa1248960:intron 30.0%
GAACAACAACAACAACAAAA+TGG - chr8_2:43329973-43329992 None:intergenic 30.0%
!! TTCTTCTTCAAAAGCATTGT+GGG + chr8_2:43329990-43330009 Msa1248960:intron 30.0%
GAGAATGTTTCGTATATACA+AGG + chr8_2:43330032-43330051 Msa1248960:intron 30.0%
CTTGAACTATTGATGAAAAG+AGG + chr8_2:43330113-43330132 Msa1248960:intron 30.0%
! CTTGTGTTGCTATATAAATG+AGG + chr8_2:43330239-43330258 Msa1248960:intron 30.0%
GAATAACCTCTCTTAGTAAA+GGG - chr8_2:43330360-43330379 None:intergenic 30.0%
CACTAAAAAACAACCTTGTT+TGG - chr8_2:43330425-43330444 None:intergenic 30.0%
!! CAAGATCAGTAGAGTTTTAA+TGG - chr8_2:43330456-43330475 None:intergenic 30.0%
TATAGACAAAATACTCACAG+TGG - chr8_2:43330775-43330794 None:intergenic 30.0%
GTTTCATAATCTGAATTGTC+AGG - chr8_2:43330893-43330912 None:intergenic 30.0%
TAATGTAGATCACTATTGAC+TGG + chr8_2:43330960-43330979 Msa1248960:intron 30.0%
TGAATTGTCAGATAATGATG+AGG + chr8_2:43331110-43331129 Msa1248960:intron 30.0%
TCAAAATCCTCATCATACAA+TGG - chr8_2:43331187-43331206 None:intergenic 30.0%
!! GAGGATTTTGATGATTATGA+AGG + chr8_2:43331196-43331215 Msa1248960:CDS 30.0%
! TTTGATGATTATGAAGGTCA+AGG + chr8_2:43331202-43331221 Msa1248960:CDS 30.0%
TCAAAATGATTGTGGGTATG+GGG - chr8_2:43328493-43328512 None:intergenic 35.0%
AATTAGTGAGAGAGATGATG+AGG + chr8_2:43328582-43328601 Msa1248960:five_prime_UTR 35.0%
ATTAGTGAGAGAGATGATGA+GGG + chr8_2:43328583-43328602 Msa1248960:five_prime_UTR 35.0%
GAGAAAAAGGATTGATTGAG+AGG + chr8_2:43328613-43328632 Msa1248960:five_prime_UTR 35.0%
CAAGATGAGGTATAAACCAT+GGG + chr8_2:43328653-43328672 Msa1248960:five_prime_UTR 35.0%
AATAGATGAGAATCCACCAT+TGG + chr8_2:43328705-43328724 Msa1248960:five_prime_UTR 35.0%
GTTCACCTTGAAGAGTAATT+TGG + chr8_2:43328738-43328757 Msa1248960:five_prime_UTR 35.0%
GTAGATCCTTTATGCATATG+TGG + chr8_2:43328959-43328978 Msa1248960:intron 35.0%
CAAACACCACATATGCATAA+AGG - chr8_2:43328968-43328987 None:intergenic 35.0%
CACAAGCAATAACCACATTA+AGG - chr8_2:43329053-43329072 None:intergenic 35.0%
ATTGACCACCAAGTAAGTAA+TGG + chr8_2:43329088-43329107 Msa1248960:intron 35.0%
TTGACCACCAAGTAAGTAAT+GGG + chr8_2:43329089-43329108 Msa1248960:intron 35.0%
AGATACACCCATTACTTACT+TGG - chr8_2:43329099-43329118 None:intergenic 35.0%
AGTTCTCTGTCTATATACGA+CGG + chr8_2:43329323-43329342 Msa1248960:intron 35.0%
ATCTCCAATCTTACCAAAAG+AGG + chr8_2:43329393-43329412 Msa1248960:intron 35.0%
!! GGATTGCTATCTGTTTTAGA+AGG - chr8_2:43329596-43329615 None:intergenic 35.0%
! ATAGCAATCCCCACAATTTT+TGG + chr8_2:43329606-43329625 Msa1248960:intron 35.0%
! TAGCAATCCCCACAATTTTT+GGG + chr8_2:43329607-43329626 Msa1248960:intron 35.0%
AGTTAACTCAGGCAATGAAA+CGG + chr8_2:43329769-43329788 Msa1248960:intron 35.0%
!! AATAATGTTCCTTTGAGGTG+TGG - chr8_2:43329888-43329907 None:intergenic 35.0%
GTGGAAATAATGTTCCTTTG+AGG - chr8_2:43329893-43329912 None:intergenic 35.0%
CATTATTTCCACTACTGAAG+TGG + chr8_2:43329901-43329920 Msa1248960:intron 35.0%
!! GTTCTTCTTCAAAAGCATTG+TGG + chr8_2:43329989-43330008 Msa1248960:intron 35.0%
!! AAAAGCATTGTGGGAAAGAT+GGG + chr8_2:43329999-43330018 Msa1248960:intron 35.0%
TTAATGCAGAGATATCTCCT+TGG + chr8_2:43330306-43330325 Msa1248960:intron 35.0%
GGAATAACCTCTCTTAGTAA+AGG - chr8_2:43330361-43330380 None:intergenic 35.0%
ATAAGTGCTCATTCCAAACA+AGG + chr8_2:43330409-43330428 Msa1248960:five_prime_UTR 35.0%
!! AACTGTTTCTATCTGCTACA+TGG + chr8_2:43330646-43330665 Msa1248960:intron 35.0%
TTCTATCTGCTACATGGTAA+TGG + chr8_2:43330652-43330671 Msa1248960:intron 35.0%
TTATGACCTCTACACTGTAA+CGG + chr8_2:43330711-43330730 Msa1248960:five_prime_UTR 35.0%
TATGAAACTGACGACTCAAA+TGG + chr8_2:43330905-43330924 Msa1248960:five_prime_UTR 35.0%
GTAAAGATAAGAATAGGCAC+AGG - chr8_2:43330937-43330956 None:intergenic 35.0%
!! TAACTTCGTCGTTTTGCTAA+AGG + chr8_2:43331148-43331167 Msa1248960:intron 35.0%
TTCTGATCCATTGTATGATG+AGG + chr8_2:43331177-43331196 Msa1248960:CDS 35.0%
AGGTAGTGAGAACAAGATGA+GGG - chr8_2:43328456-43328475 None:intergenic 40.0%
TGATTGAGAGGTAGAAAGAG+AGG + chr8_2:43328625-43328644 Msa1248960:five_prime_UTR 40.0%
GGTTTATACCTCATCTTGGT+CGG - chr8_2:43328651-43328670 None:intergenic 40.0%
CCATGGTTTATACCTCATCT+TGG - chr8_2:43328655-43328674 None:intergenic 40.0%
CCAAGATGAGGTATAAACCA+TGG + chr8_2:43328652-43328671 Msa1248960:five_prime_UTR 40.0%
AATCCACCATTGGAGAGATT+AGG + chr8_2:43328715-43328734 Msa1248960:five_prime_UTR 40.0%
ATCCACCATTGGAGAGATTA+GGG + chr8_2:43328716-43328735 Msa1248960:five_prime_UTR 40.0%
TACACCCATTACTTACTTGG+TGG - chr8_2:43329096-43329115 None:intergenic 40.0%
GTTCTCTGTCTATATACGAC+GGG + chr8_2:43329324-43329343 Msa1248960:intron 40.0%
CACGCTTTATGACTTAGACA+AGG + chr8_2:43329478-43329497 Msa1248960:intron 40.0%
GCTTTATGACTTAGACAAGG+AGG + chr8_2:43329481-43329500 Msa1248960:intron 40.0%
! AGCAATCCCCACAATTTTTG+GGG + chr8_2:43329608-43329627 Msa1248960:intron 40.0%
! AACAACAGTGCTACTATTCG+TGG - chr8_2:43329804-43329823 None:intergenic 40.0%
GTGGTGCATTACCATCAAAT+GGG - chr8_2:43329869-43329888 None:intergenic 40.0%
TGTGGTGCATTACCATCAAA+TGG - chr8_2:43329870-43329889 None:intergenic 40.0%
CAACAAAATGGCTGAAGCTT+CGG - chr8_2:43329961-43329980 None:intergenic 40.0%
!! CAAAAGCATTGTGGGAAAGA+TGG + chr8_2:43329998-43330017 Msa1248960:intron 40.0%
!! TGGATTTATATGGAGGTGCT+TGG + chr8_2:43330089-43330108 Msa1248960:intron 40.0%
!! GGATTTATATGGAGGTGCTT+GGG + chr8_2:43330090-43330109 Msa1248960:intron 40.0%
CAAAAGTCTTTGATCCTCCA+AGG - chr8_2:43330326-43330345 None:intergenic 40.0%
TTTCCTCCCTTTACTAAGAG+AGG + chr8_2:43330351-43330370 Msa1248960:five_prime_UTR 40.0%
TAACCTCTCTTAGTAAAGGG+AGG - chr8_2:43330357-43330376 None:intergenic 40.0%
GGTGTATGTGACATATACAG+AGG + chr8_2:43330681-43330700 Msa1248960:intron 40.0%
TCGTCCGGATAATCATTCAT+TGG - chr8_2:43331024-43331043 None:intergenic 40.0%
TGATTATCCGGACGAGATTT+CGG + chr8_2:43331029-43331048 Msa1248960:CDS 40.0%
GAGATTTCGGAAGATGAAGA+AGG + chr8_2:43331042-43331061 Msa1248960:CDS 40.0%
TCAGAAGAAAGCAAGCATAG+CGG + chr8_2:43331078-43331097 Msa1248960:CDS 40.0%
GTCAGATAATGATGAGGATG+TGG + chr8_2:43331116-43331135 Msa1248960:intron 40.0%
GATTATGAAGGTCAAGGTGT+AGG + chr8_2:43331208-43331227 Msa1248960:CDS 40.0%
TGTAGGCTATGACAATGATG+AGG + chr8_2:43331225-43331244 Msa1248960:CDS 40.0%
CTATGACAATGATGAGGATG+AGG + chr8_2:43331231-43331250 Msa1248960:CDS 40.0%
CAGGTAGTGAGAACAAGATG+AGG - chr8_2:43328457-43328476 None:intergenic 45.0%
CTCATCTTGTTCTCACTACC+TGG + chr8_2:43328455-43328474 Msa1248960:five_prime_UTR 45.0%
TGAGGGCTGAAGAGAGAAAA+AGG + chr8_2:43328600-43328619 Msa1248960:five_prime_UTR 45.0%
TGAGGTATAAACCATGGGAG+AGG + chr8_2:43328658-43328677 Msa1248960:five_prime_UTR 45.0%
AAATTTCAGCTCCTCTCCCA+TGG - chr8_2:43328672-43328691 None:intergenic 45.0%
AACCCTAATCTCTCCAATGG+TGG - chr8_2:43328721-43328740 None:intergenic 45.0%
GTGAACCCTAATCTCTCCAA+TGG - chr8_2:43328724-43328743 None:intergenic 45.0%
GACAGAGAACTCACCTTACA+AGG - chr8_2:43329314-43329333 None:intergenic 45.0%
TCTGTCTATATACGACGGGA+GGG + chr8_2:43329328-43329347 Msa1248960:intron 45.0%
!! GCAATCCCCACAATTTTTGG+GGG + chr8_2:43329609-43329628 Msa1248960:intron 45.0%
ATGCCACCCCCAAAAATTGT+GGG - chr8_2:43329618-43329637 None:intergenic 45.0%
TATGCCACCCCCAAAAATTG+TGG - chr8_2:43329619-43329638 None:intergenic 45.0%
!! CAATTTTTGGGGGTGGCATA+AGG + chr8_2:43329619-43329638 Msa1248960:intron 45.0%
! GCTACTATTCGTGGTGGTTT+TGG - chr8_2:43329795-43329814 None:intergenic 45.0%
! AACAGTGCTACTATTCGTGG+TGG - chr8_2:43329801-43329820 None:intergenic 45.0%
ATGCAGAGATATCTCCTTGG+AGG + chr8_2:43330309-43330328 Msa1248960:intron 45.0%
!! GCTACATGGTAATGGTGCAT+TGG + chr8_2:43330660-43330679 Msa1248960:intron 45.0%
TCTCATCCGTTACAGTGTAG+AGG - chr8_2:43330720-43330739 None:intergenic 45.0%
TCATCTTCCGAAATCTCGTC+CGG - chr8_2:43331039-43331058 None:intergenic 45.0%
CAATGATGAGGATGAGGATG+AGG + chr8_2:43331237-43331256 Msa1248960:CDS 45.0%
TGAGGATGAGGATGAAGATG+TGG + chr8_2:43331249-43331268 Msa1248960:CDS 45.0%
GAGGATGAGGATGAAGATGT+GGG + chr8_2:43331250-43331269 Msa1248960:CDS 45.0%
TGATTGTGGGTATGGGGTGA+AGG - chr8_2:43328487-43328506 None:intergenic 50.0%
CTCTGTCTATATACGACGGG+AGG + chr8_2:43329327-43329346 Msa1248960:intron 50.0%
CTGTCTATATACGACGGGAG+GGG + chr8_2:43329329-43329348 Msa1248960:intron 50.0%
!! ATCCCCACAATTTTTGGGGG+TGG + chr8_2:43329612-43329631 Msa1248960:intron 50.0%
TGCCACCCCCAAAAATTGTG+GGG - chr8_2:43329617-43329636 None:intergenic 50.0%
GGGAGCGAGCAATTTCTGTT+GGG - chr8_2:43329720-43329739 None:intergenic 50.0%
TGGGAGCGAGCAATTTCTGT+TGG - chr8_2:43329721-43329740 None:intergenic 50.0%
GGTAATGCACCACACCTCAA+AGG + chr8_2:43329876-43329895 Msa1248960:intron 50.0%
!! CTTCAGTGCCACTTCAGTAG+TGG - chr8_2:43329912-43329931 None:intergenic 50.0%
TATTCCTAATGCCGCTGCAG+AGG + chr8_2:43330375-43330394 Msa1248960:five_prime_UTR 50.0%
ATCAGCTTCAACCTCTGCAG+CGG - chr8_2:43330389-43330408 None:intergenic 50.0%
ATGGGGTGAAGGATGGTACC+AGG - chr8_2:43328476-43328495 None:intergenic 55.0%
TGTGGGTATGGGGTGAAGGA+TGG - chr8_2:43328483-43328502 None:intergenic 55.0%
AAGAGAGGCCGACCAAGATG+AGG + chr8_2:43328640-43328659 Msa1248960:five_prime_UTR 55.0%
TGCCTGAGTTAACTCGCGCA+GGG - chr8_2:43329763-43329782 None:intergenic 55.0%
TTGCCTGAGTTAACTCGCGC+AGG - chr8_2:43329764-43329783 None:intergenic 55.0%
TCAACCTCTGCAGCGGCATT+AGG - chr8_2:43330382-43330401 None:intergenic 55.0%
GGGGTAAGCGAGGGTAAGCA+AGG + chr8_2:43329348-43329367 Msa1248960:intron 60.0%
GCCTGAGTTAACTCGCGCAG+GGG - chr8_2:43329762-43329781 None:intergenic 60.0%
TACGACGGGAGGGGTAAGCG+AGG + chr8_2:43329338-43329357 Msa1248960:intron 65.0%
ACGACGGGAGGGGTAAGCGA+GGG + chr8_2:43329339-43329358 Msa1248960:intron 65.0%
GCCCCTGCGCGAGTTAACTC+AGG + chr8_2:43329758-43329777 Msa1248960:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 43328434 43331285 43328434 ID=Msa1248960;Name=Msa1248960
chr8_2 mRNA 43328434 43331285 43328434 ID=Msa1248960-mRNA-1;Parent=Msa1248960;Name=Msa1248960-mRNA-1;_AED=0.25;_eAED=0.25;_QI=641|0.8|0.66|1|0.8|0.83|6|0|65
chr8_2 exon 43328434 43328797 43328434 ID=Msa1248960-mRNA-1:exon:13201;Parent=Msa1248960-mRNA-1
chr8_2 exon 43330315 43330430 43330315 ID=Msa1248960-mRNA-1:exon:13202;Parent=Msa1248960-mRNA-1
chr8_2 exon 43330701 43330776 43330701 ID=Msa1248960-mRNA-1:exon:13203;Parent=Msa1248960-mRNA-1
chr8_2 exon 43330856 43330926 43330856 ID=Msa1248960-mRNA-1:exon:13204;Parent=Msa1248960-mRNA-1
chr8_2 exon 43331010 43331105 43331010 ID=Msa1248960-mRNA-1:exon:13205;Parent=Msa1248960-mRNA-1
chr8_2 exon 43331170 43331285 43331170 ID=Msa1248960-mRNA-1:exon:13206;Parent=Msa1248960-mRNA-1
chr8_2 five_prime_UTR 43328434 43328797 43328434 ID=Msa1248960-mRNA-1:five_prime_utr;Parent=Msa1248960-mRNA-1
chr8_2 five_prime_UTR 43330315 43330430 43330315 ID=Msa1248960-mRNA-1:five_prime_utr;Parent=Msa1248960-mRNA-1
chr8_2 five_prime_UTR 43330701 43330776 43330701 ID=Msa1248960-mRNA-1:five_prime_utr;Parent=Msa1248960-mRNA-1
chr8_2 five_prime_UTR 43330856 43330926 43330856 ID=Msa1248960-mRNA-1:five_prime_utr;Parent=Msa1248960-mRNA-1
chr8_2 five_prime_UTR 43331010 43331023 43331010 ID=Msa1248960-mRNA-1:five_prime_utr;Parent=Msa1248960-mRNA-1
chr8_2 CDS 43331024 43331105 43331024 ID=Msa1248960-mRNA-1:cds;Parent=Msa1248960-mRNA-1
chr8_2 CDS 43331170 43331285 43331170 ID=Msa1248960-mRNA-1:cds;Parent=Msa1248960-mRNA-1
Gene Sequence

>Msa1248960

ATGAATGATTATCCGGACGAGATTTCGGAAGATGAAGAAGGTTCTGAGTATGAATCAGAAGAAAGCAAGCATAGCGGATCTAGTCTTACTTCTGATCCATTGTATGATGAGGATTTTGATGATTATGAAGGTCAAGGTGTAGGCTATGACAATGATGAGGATGAGGATGAGGATGAAGATGTGGGTAATGAGCATTGA
Protein sequence

>Msa1248960

MNDYPDEISEDEEGSEYESEESKHSGSSLTSDPLYDEDFDDYEGQGVGYDNDEDEDEDEDVGNEH*