AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1195600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1195600 MtrunA17_Chr7g0227381 84.507 71 6 2 1 66 280 350 1.20e-26 99.0
Msa1195600 MtrunA17_Chr8g0364491 83.824 68 8 2 1 66 275 341 3.42e-17 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 66 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCACCTTGAAGAGTAATT+TGG 0.164526 8_1:+43745395 Msa1195600:five_prime_UTR
TGATTATCCGGACGAGATTT+CGG 0.287103 8_1:+43747676 Msa1195600:CDS
TGAGGGCTGAAGAGAGAAAA+AGG 0.293209 8_1:+43745257 Msa1195600:five_prime_UTR
AATCAAAATGATTGTGGGTA+TGG 0.340235 8_1:-43745149 None:intergenic
AATTCCCAAATTACTCTTCA+AGG 0.343203 8_1:-43745400 None:intergenic
TCAACCTCTGCAGCGGCATT+AGG 0.347281 8_1:-43747026 None:intergenic
TTCACCTTGAAGAGTAATTT+GGG 0.360202 8_1:+43745396 Msa1195600:five_prime_UTR
ATCCACCATTGGAGAGATTA+GGG 0.361540 8_1:+43745373 Msa1195600:five_prime_UTR
CTTCTCTCAAGAAACAAATA+AGG 0.370343 8_1:+43745433 Msa1195600:five_prime_UTR
TTCACTTCTTCAATAAATCA+AGG 0.409052 8_1:+43745216 Msa1195600:five_prime_UTR
AATCCACCATTGGAGAGATT+AGG 0.423711 8_1:+43745372 Msa1195600:five_prime_UTR
AAATCCAATGAATGATTATC+CGG 0.442829 8_1:+43747664 Msa1195600:exon
CCATGGTTTATACCTCATCT+TGG 0.452908 8_1:-43745309 None:intergenic
GGAATAACCTCTCTTAGTAA+AGG 0.453029 8_1:-43747005 None:intergenic
TACAATAATCAAAATGATTG+TGG 0.455475 8_1:-43745155 None:intergenic
ACAATAATCAAAATGATTGT+GGG 0.461891 8_1:-43745154 None:intergenic
GAATAACCTCTCTTAGTAAA+GGG 0.463078 8_1:-43747004 None:intergenic
CTCATCTTGTTCTCACTACC+TGG 0.463111 8_1:+43745112 Msa1195600:five_prime_UTR
ATCAAAATGATTGTGGGTAT+GGG 0.479465 8_1:-43745148 None:intergenic
GTTTCACAATCTGAATTCTC+AGG 0.481064 8_1:-43747537 None:intergenic
AATTAGTGAGAGAGATGATG+AGG 0.481100 8_1:+43745239 Msa1195600:five_prime_UTR
TCGTCCGGATAATCATTCAT+TGG 0.491166 8_1:-43747668 None:intergenic
TGTGGGTATGGGGTGAAGGA+TGG 0.492753 8_1:-43745137 None:intergenic
TGATTGTGGGTATGGGGTGA+AGG 0.503876 8_1:-43745141 None:intergenic
TCATCTTCCGAAATCTCGTC+CGG 0.506670 8_1:-43747683 None:intergenic
TGTGAAACTGACGACTCAAA+TGG 0.514225 8_1:+43747552 Msa1195600:five_prime_UTR
GGTTTATACCTCATCTTGGT+CGG 0.519331 8_1:-43745305 None:intergenic
TTCTGATCCATTGTATGATG+AGG 0.519454 8_1:+43747814 Msa1195600:CDS
TTTCCTCCCTTTACTAAGAG+AGG 0.528946 8_1:+43746998 Msa1195600:five_prime_UTR
AAATTTCAGCTCCTCTCCCA+TGG 0.529808 8_1:-43745326 None:intergenic
GAGATTTCGGAAGATGAAGA+AGG 0.532402 8_1:+43747689 Msa1195600:CDS
TTTGATGATTATGAAGGTCA+AGG 0.533767 8_1:+43747839 Msa1195600:CDS
GAATACCCAGATGGCTTCAC+CGG 0.534046 8_1:-43747929 None:intergenic
ATGCAGAGATATCTCCTTGG+AGG 0.535146 8_1:+43746956 Msa1195600:intron
ATGTGGGTAATGAGCATTGA+AGG 0.543289 8_1:+43747898 Msa1195600:three_prime_UTR
ATGGGGTGAAGGATGGTACC+AGG 0.551102 8_1:-43745130 None:intergenic
ATTAGTGAGAGAGATGATGA+GGG 0.556768 8_1:+43745240 Msa1195600:five_prime_UTR
CAAAAGTCTTTGATCCTCCA+AGG 0.567079 8_1:-43746970 None:intergenic
GTGAACCCTAATCTCTCCAA+TGG 0.570926 8_1:-43745378 None:intergenic
GATTATGAAGGTCAAGGTGT+AGG 0.571993 8_1:+43747845 Msa1195600:CDS
CGAATTTATGAATACCCAGA+TGG 0.576058 8_1:-43747938 None:intergenic
AACCCTAATCTCTCCAATGG+TGG 0.586333 8_1:-43745375 None:intergenic
TCAAAATCCTCATCATACAA+TGG 0.590172 8_1:-43747821 None:intergenic
TATTCCTAATGCCGCTGCAG+AGG 0.592055 8_1:+43747022 Msa1195600:five_prime_UTR
TTATGACAATGATGAGGATG+AGG 0.599856 8_1:+43747869 Msa1195600:exon
TGATTGAGAGGTAGAAAGAG+AGG 0.600610 8_1:+43745282 Msa1195600:five_prime_UTR
TGAGGATGAGGATGAAGATG+TGG 0.600853 8_1:+43747881 Msa1195600:three_prime_UTR
AATAGATGAGAATCCACCAT+TGG 0.601690 8_1:+43745362 Msa1195600:five_prime_UTR
TTATGACCTCTACACTGTAA+CGG 0.604149 8_1:+43747358 Msa1195600:five_prime_UTR
CCAAGATGAGGTATAAACCA+TGG 0.609551 8_1:+43745309 Msa1195600:five_prime_UTR
TGAGGTATAAACCATGGGAG+AGG 0.612118 8_1:+43745315 Msa1195600:five_prime_UTR
CAAGATGAGGTATAAACCAT+GGG 0.615549 8_1:+43745310 Msa1195600:five_prime_UTR
TCAAAATGATTGTGGGTATG+GGG 0.616761 8_1:-43745147 None:intergenic
AAGAGAGGCCGACCAAGATG+AGG 0.618165 8_1:+43745297 Msa1195600:five_prime_UTR
AGGTAGTGAGAACAAGATGA+GGG 0.624482 8_1:-43745110 None:intergenic
ATAAGTGCTCATTCCAAACA+AGG 0.626145 8_1:+43747056 Msa1195600:five_prime_UTR
TAACCTCTCTTAGTAAAGGG+AGG 0.626192 8_1:-43747001 None:intergenic
TTAATGCAGAGATATCTCCT+TGG 0.630700 8_1:+43746953 Msa1195600:intron
ATCAGCTTCAACCTCTGCAG+CGG 0.637554 8_1:-43747033 None:intergenic
CAGGTAGTGAGAACAAGATG+AGG 0.637789 8_1:-43745111 None:intergenic
TCTCATCCGTTACAGTGTAG+AGG 0.640074 8_1:-43747364 None:intergenic
GAGGATGAGGATGAAGATGT+GGG 0.642593 8_1:+43747882 Msa1195600:three_prime_UTR
TGGGTAATGAGCATTGAAGG+TGG 0.645185 8_1:+43747901 Msa1195600:three_prime_UTR
TGAACTGTCAGATAATGATG+AGG 0.719056 8_1:+43747757 Msa1195600:CDS
GTAGGCTTATGACAATGATG+AGG 0.735441 8_1:+43747863 Msa1195600:exon
TCAGAAGAAAGCAAGCATAG+CGG 0.746054 8_1:+43747725 Msa1195600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATGTTAACTTAATAA+TGG + chr8_1:43745594-43745613 Msa1195600:intron 10.0%
!! AATATCTTCAATAATAAATA+AGG + chr8_1:43746812-43746831 Msa1195600:intron 10.0%
!! CATATAAATCCAAAAAAATT+AGG - chr8_1:43746728-43746747 None:intergenic 15.0%
!!! CTAATTTTTTTGGATTTATA+TGG + chr8_1:43746726-43746745 Msa1195600:intron 15.0%
!! AAAAAAATAAGTGAAACTTA+AGG - chr8_1:43747135-43747154 None:intergenic 15.0%
!!! TAAATAATTGATGTATTTTC+TGG + chr8_1:43747158-43747177 Msa1195600:intron 15.0%
!!! AAATAATTGATGTATTTTCT+GGG + chr8_1:43747159-43747178 Msa1195600:intron 15.0%
!! ACAATAATCAAAATGATTGT+GGG - chr8_1:43745157-43745176 None:intergenic 20.0%
!! TACAATAATCAAAATGATTG+TGG - chr8_1:43745158-43745177 None:intergenic 20.0%
!!! TTTTTGAAATAAGACATGAA+TGG + chr8_1:43745794-43745813 Msa1195600:intron 20.0%
!! TATTAGTAAGTGAGAATAAT+AGG + chr8_1:43745933-43745952 Msa1195600:intron 20.0%
!!! TTATCTTGACCTAATTTTTT+TGG + chr8_1:43746716-43746735 Msa1195600:intron 20.0%
!!! ATTTTTTTGGATTTATATGG+AGG + chr8_1:43746729-43746748 Msa1195600:intron 20.0%
!!! CAATAAAGACAAACATATTT+TGG + chr8_1:43747183-43747202 Msa1195600:intron 20.0%
!! TTTGTCTATACATGTTTAAT+CGG + chr8_1:43747432-43747451 Msa1195600:intron 20.0%
!!! ATGAAGAAGATTATTTTGAT+GGG + chr8_1:43747514-43747533 Msa1195600:five_prime_UTR 20.0%
!! TTAAGAGTAAAGATAAGAAT+AGG - chr8_1:43747590-43747609 None:intergenic 20.0%
! TTCACTTCTTCAATAAATCA+AGG + chr8_1:43745216-43745235 Msa1195600:five_prime_UTR 25.0%
!!! ATCTTTTAGAGGTTTTAATG+GGG - chr8_1:43745470-43745489 None:intergenic 25.0%
!!! CATCTTTTAGAGGTTTTAAT+GGG - chr8_1:43745471-43745490 None:intergenic 25.0%
!!! ACATCTTTTAGAGGTTTTAA+TGG - chr8_1:43745472-43745491 None:intergenic 25.0%
!! ATAGATCAGTAAGAGTTTTA+AGG + chr8_1:43745571-43745590 Msa1195600:intron 25.0%
! TGTGTGAAATCATGATATTA+TGG + chr8_1:43745655-43745674 Msa1195600:intron 25.0%
! TTTCGACTTAAACTTTAGTT+TGG - chr8_1:43746206-43746225 None:intergenic 25.0%
! ACTAAAGTTTAAGTCGAAAT+TGG + chr8_1:43746207-43746226 Msa1195600:intron 25.0%
! CTAAAGTTTAAGTCGAAATT+GGG + chr8_1:43746208-43746227 Msa1195600:intron 25.0%
! GATTACGAGTCATAAATTTA+GGG - chr8_1:43746302-43746321 None:intergenic 25.0%
! AGCAGAATTCTTAAAATTGT+GGG - chr8_1:43746342-43746361 None:intergenic 25.0%
!!! GCATTTTAGTATTTTCAGTT+GGG - chr8_1:43746390-43746409 None:intergenic 25.0%
! AACTTAAATAACCCATTTGA+TGG + chr8_1:43746502-43746521 Msa1195600:intron 25.0%
! TTGAACTATTGATGAAAAGA+GGG + chr8_1:43746761-43746780 Msa1195600:intron 25.0%
!! TTGTGTTGCTATATAAATGA+GGG + chr8_1:43746887-43746906 Msa1195600:intron 25.0%
!! CAAGATCAGTAGAATTTTAA+TGG - chr8_1:43747103-43747122 None:intergenic 25.0%
! TTGTCTATACATGTTTAATC+GGG + chr8_1:43747433-43747452 Msa1195600:intron 25.0%
!!! GATGAAGAAGATTATTTTGA+TGG + chr8_1:43747513-43747532 Msa1195600:five_prime_UTR 25.0%
! AAATCCAATGAATGATTATC+CGG + chr8_1:43747664-43747683 Msa1195600:exon 25.0%
ATCAAAATGATTGTGGGTAT+GGG - chr8_1:43745151-43745170 None:intergenic 30.0%
AATCAAAATGATTGTGGGTA+TGG - chr8_1:43745152-43745171 None:intergenic 30.0%
! CTTTGCATGTTTTGTCATAT+TGG - chr8_1:43745189-43745208 None:intergenic 30.0%
TTCACCTTGAAGAGTAATTT+GGG + chr8_1:43745396-43745415 Msa1195600:five_prime_UTR 30.0%
AATTCCCAAATTACTCTTCA+AGG - chr8_1:43745403-43745422 None:intergenic 30.0%
CTTCTCTCAAGAAACAAATA+AGG + chr8_1:43745433-43745452 Msa1195600:five_prime_UTR 30.0%
! AATCTAAGCACATCTTTTAG+AGG - chr8_1:43745481-43745500 None:intergenic 30.0%
GAATCAATCACATGCAAATA+GGG - chr8_1:43745538-43745557 None:intergenic 30.0%
AGAATCAATCACATGCAAAT+AGG - chr8_1:43745539-43745558 None:intergenic 30.0%
! TAATTTTGCATGCCTTAATG+TGG + chr8_1:43745695-43745714 Msa1195600:intron 30.0%
ATGGAGTAGATGATTATTCA+AGG + chr8_1:43745813-43745832 Msa1195600:intron 30.0%
TGGAGTAGATGATTATTCAA+GGG + chr8_1:43745814-43745833 Msa1195600:intron 30.0%
TAATAGGATTCATCCTTGTA+AGG + chr8_1:43745949-43745968 Msa1195600:intron 30.0%
! AGTTTTCTGTCTATATACGA+CGG + chr8_1:43745974-43745993 Msa1195600:intron 30.0%
! TTAACCTCTTTTGGTAAGAT+TGG - chr8_1:43746051-43746070 None:intergenic 30.0%
!!! CTCATAGTTTTAACCTCTTT+TGG - chr8_1:43746060-43746079 None:intergenic 30.0%
CAAAAGAGGTTAAAACTATG+AGG + chr8_1:43746058-43746077 Msa1195600:intron 30.0%
ACAAGGAGGTTAAATTTAAG+AGG + chr8_1:43746146-43746165 Msa1195600:intron 30.0%
TATGAAACCCCAAAAATTGT+GGG - chr8_1:43746269-43746288 None:intergenic 30.0%
TTATGAAACCCCAAAAATTG+TGG - chr8_1:43746270-43746289 None:intergenic 30.0%
!!! ACAATTTTTGGGGTTTCATA+AGG + chr8_1:43746269-43746288 Msa1195600:intron 30.0%
CGATTACGAGTCATAAATTT+AGG - chr8_1:43746303-43746322 None:intergenic 30.0%
GCAGAATTCTTAAAATTGTG+GGG - chr8_1:43746341-43746360 None:intergenic 30.0%
GAGCAGAATTCTTAAAATTG+TGG - chr8_1:43746343-43746362 None:intergenic 30.0%
!! GGCATTTTAGTATTTTCAGT+TGG - chr8_1:43746391-43746410 None:intergenic 30.0%
!!! ATGATCATCATCACTTTTTG+AGG + chr8_1:43746478-43746497 Msa1195600:intron 30.0%
GAACAACAACAACAACAAAA+TGG - chr8_1:43746620-43746639 None:intergenic 30.0%
!! TTCTTCTTCAAAAGCATTGT+GGG + chr8_1:43746637-43746656 Msa1195600:intron 30.0%
AGAATGTTTCGTATACACAA+GGG + chr8_1:43746680-43746699 Msa1195600:intron 30.0%
CTTGAACTATTGATGAAAAG+AGG + chr8_1:43746760-43746779 Msa1195600:intron 30.0%
! CTTGTGTTGCTATATAAATG+AGG + chr8_1:43746886-43746905 Msa1195600:intron 30.0%
GAATAACCTCTCTTAGTAAA+GGG - chr8_1:43747007-43747026 None:intergenic 30.0%
CACTAAAAAACAACCTTGTT+TGG - chr8_1:43747072-43747091 None:intergenic 30.0%
TATAGACAAAATACTCACAG+TGG - chr8_1:43747422-43747441 None:intergenic 30.0%
TAATGTAGATCACTATTGAC+TGG + chr8_1:43747607-43747626 Msa1195600:intron 30.0%
TCAAAATCCTCATCATACAA+TGG - chr8_1:43747824-43747843 None:intergenic 30.0%
!! GAGGATTTTGATGATTATGA+AGG + chr8_1:43747833-43747852 Msa1195600:CDS 30.0%
! TTTGATGATTATGAAGGTCA+AGG + chr8_1:43747839-43747858 Msa1195600:CDS 30.0%
TCAAAATGATTGTGGGTATG+GGG - chr8_1:43745150-43745169 None:intergenic 35.0%
AATTAGTGAGAGAGATGATG+AGG + chr8_1:43745239-43745258 Msa1195600:five_prime_UTR 35.0%
ATTAGTGAGAGAGATGATGA+GGG + chr8_1:43745240-43745259 Msa1195600:five_prime_UTR 35.0%
GAGAAAAAGGATTGATTGAG+AGG + chr8_1:43745270-43745289 Msa1195600:five_prime_UTR 35.0%
CAAGATGAGGTATAAACCAT+GGG + chr8_1:43745310-43745329 Msa1195600:five_prime_UTR 35.0%
AATAGATGAGAATCCACCAT+TGG + chr8_1:43745362-43745381 Msa1195600:five_prime_UTR 35.0%
GTTCACCTTGAAGAGTAATT+TGG + chr8_1:43745395-43745414 Msa1195600:five_prime_UTR 35.0%
GTAGATCCTTTATGCATATG+TGG + chr8_1:43745616-43745635 Msa1195600:intron 35.0%
CAAACACCACATATGCATAA+AGG - chr8_1:43745625-43745644 None:intergenic 35.0%
CACAAGCAATAACCACATTA+AGG - chr8_1:43745710-43745729 None:intergenic 35.0%
ATTGACCACCAAGTAAGTAA+TGG + chr8_1:43745745-43745764 Msa1195600:intron 35.0%
TTGACCACCAAGTAAGTAAT+GGG + chr8_1:43745746-43745765 Msa1195600:intron 35.0%
AGATACACCCATTACTTACT+TGG - chr8_1:43745756-43745775 None:intergenic 35.0%
! GTTTTCTGTCTATATACGAC+GGG + chr8_1:43745975-43745994 Msa1195600:intron 35.0%
ATCTCCAATCTTACCAAAAG+AGG + chr8_1:43746044-43746063 Msa1195600:intron 35.0%
!! GGATTGCTATCTGTTTTAGA+AGG - chr8_1:43746247-43746266 None:intergenic 35.0%
! ATAGCAATCCCCACAATTTT+TGG + chr8_1:43746257-43746276 Msa1195600:intron 35.0%
! TAGCAATCCCCACAATTTTT+GGG + chr8_1:43746258-43746277 Msa1195600:intron 35.0%
ATGAAACCCCAAAAATTGTG+GGG - chr8_1:43746268-43746287 None:intergenic 35.0%
AGTTAACTCAGGCAATGAAA+CGG + chr8_1:43746419-43746438 Msa1195600:intron 35.0%
!! AATAATGTTCCTTTGAGGTG+TGG - chr8_1:43746535-43746554 None:intergenic 35.0%
GTGGAAATAATGTTCCTTTG+AGG - chr8_1:43746540-43746559 None:intergenic 35.0%
CATTATTTCCACTACTGAAG+TGG + chr8_1:43746548-43746567 Msa1195600:intron 35.0%
!! GTTCTTCTTCAAAAGCATTG+TGG + chr8_1:43746636-43746655 Msa1195600:intron 35.0%
!! AAAAGCATTGTGGGAAAGAT+GGG + chr8_1:43746646-43746665 Msa1195600:intron 35.0%
GAGAATGTTTCGTATACACA+AGG + chr8_1:43746679-43746698 Msa1195600:intron 35.0%
TTAATGCAGAGATATCTCCT+TGG + chr8_1:43746953-43746972 Msa1195600:intron 35.0%
GGAATAACCTCTCTTAGTAA+AGG - chr8_1:43747008-43747027 None:intergenic 35.0%
ATAAGTGCTCATTCCAAACA+AGG + chr8_1:43747056-43747075 Msa1195600:five_prime_UTR 35.0%
!! AACTGTTTCTATCTGCTACA+TGG + chr8_1:43747293-43747312 Msa1195600:intron 35.0%
TTCTATCTGCTACATGGTAA+TGG + chr8_1:43747299-43747318 Msa1195600:intron 35.0%
TTATGACCTCTACACTGTAA+CGG + chr8_1:43747358-43747377 Msa1195600:five_prime_UTR 35.0%
GTTTCACAATCTGAATTCTC+AGG - chr8_1:43747540-43747559 None:intergenic 35.0%
GTAAAGATAAGAATAGGCAC+AGG - chr8_1:43747584-43747603 None:intergenic 35.0%
TGAACTGTCAGATAATGATG+AGG + chr8_1:43747757-43747776 Msa1195600:CDS 35.0%
TTCTGATCCATTGTATGATG+AGG + chr8_1:43747814-43747833 Msa1195600:CDS 35.0%
TTATGACAATGATGAGGATG+AGG + chr8_1:43747869-43747888 Msa1195600:exon 35.0%
CGAATTTATGAATACCCAGA+TGG - chr8_1:43747941-43747960 None:intergenic 35.0%
AGGTAGTGAGAACAAGATGA+GGG - chr8_1:43745113-43745132 None:intergenic 40.0%
TGATTGAGAGGTAGAAAGAG+AGG + chr8_1:43745282-43745301 Msa1195600:five_prime_UTR 40.0%
GGTTTATACCTCATCTTGGT+CGG - chr8_1:43745308-43745327 None:intergenic 40.0%
CCATGGTTTATACCTCATCT+TGG - chr8_1:43745312-43745331 None:intergenic 40.0%
CCAAGATGAGGTATAAACCA+TGG + chr8_1:43745309-43745328 Msa1195600:five_prime_UTR 40.0%
AATCCACCATTGGAGAGATT+AGG + chr8_1:43745372-43745391 Msa1195600:five_prime_UTR 40.0%
ATCCACCATTGGAGAGATTA+GGG + chr8_1:43745373-43745392 Msa1195600:five_prime_UTR 40.0%
TACACCCATTACTTACTTGG+TGG - chr8_1:43745753-43745772 None:intergenic 40.0%
GACAGAAAACTCACCTTACA+AGG - chr8_1:43745965-43745984 None:intergenic 40.0%
CACGCTTTATGACTTAGACA+AGG + chr8_1:43746129-43746148 Msa1195600:intron 40.0%
GCTTTATGACTTAGACAAGG+AGG + chr8_1:43746132-43746151 Msa1195600:intron 40.0%
! AGCAATCCCCACAATTTTTG+GGG + chr8_1:43746259-43746278 Msa1195600:intron 40.0%
!! AGTGCTACTATTCGTGGTTT+TGG - chr8_1:43746445-43746464 None:intergenic 40.0%
! AACAACAGTGCTACTATTCG+TGG - chr8_1:43746451-43746470 None:intergenic 40.0%
GTGGTGCATTACCATCAAAT+GGG - chr8_1:43746516-43746535 None:intergenic 40.0%
TGTGGTGCATTACCATCAAA+TGG - chr8_1:43746517-43746536 None:intergenic 40.0%
CAACAAAATGGCTGAAGCTT+CGG - chr8_1:43746608-43746627 None:intergenic 40.0%
!! CAAAAGCATTGTGGGAAAGA+TGG + chr8_1:43746645-43746664 Msa1195600:intron 40.0%
!! TGGATTTATATGGAGGTGCT+TGG + chr8_1:43746736-43746755 Msa1195600:intron 40.0%
!! GGATTTATATGGAGGTGCTT+GGG + chr8_1:43746737-43746756 Msa1195600:intron 40.0%
CAAAAGTCTTTGATCCTCCA+AGG - chr8_1:43746973-43746992 None:intergenic 40.0%
TTTCCTCCCTTTACTAAGAG+AGG + chr8_1:43746998-43747017 Msa1195600:five_prime_UTR 40.0%
TAACCTCTCTTAGTAAAGGG+AGG - chr8_1:43747004-43747023 None:intergenic 40.0%
GGTGTATGTGACATATACAG+AGG + chr8_1:43747328-43747347 Msa1195600:intron 40.0%
TGTGAAACTGACGACTCAAA+TGG + chr8_1:43747552-43747571 Msa1195600:five_prime_UTR 40.0%
TCGTCCGGATAATCATTCAT+TGG - chr8_1:43747671-43747690 None:intergenic 40.0%
TGATTATCCGGACGAGATTT+CGG + chr8_1:43747676-43747695 Msa1195600:CDS 40.0%
GAGATTTCGGAAGATGAAGA+AGG + chr8_1:43747689-43747708 Msa1195600:CDS 40.0%
TCAGAAGAAAGCAAGCATAG+CGG + chr8_1:43747725-43747744 Msa1195600:CDS 40.0%
!! GAACTTCGTCGTTTTGCTAA+AGG + chr8_1:43747785-43747804 Msa1195600:CDS 40.0%
GATTATGAAGGTCAAGGTGT+AGG + chr8_1:43747845-43747864 Msa1195600:CDS 40.0%
GTAGGCTTATGACAATGATG+AGG + chr8_1:43747863-43747882 Msa1195600:exon 40.0%
!! ATGTGGGTAATGAGCATTGA+AGG + chr8_1:43747898-43747917 Msa1195600:three_prime_UTR 40.0%
!! AGCATTGAAGGTGGTTTTAC+CGG + chr8_1:43747910-43747929 Msa1195600:three_prime_UTR 40.0%
CAGGTAGTGAGAACAAGATG+AGG - chr8_1:43745114-43745133 None:intergenic 45.0%
CTCATCTTGTTCTCACTACC+TGG + chr8_1:43745112-43745131 Msa1195600:five_prime_UTR 45.0%
TGAGGGCTGAAGAGAGAAAA+AGG + chr8_1:43745257-43745276 Msa1195600:five_prime_UTR 45.0%
TGAGGTATAAACCATGGGAG+AGG + chr8_1:43745315-43745334 Msa1195600:five_prime_UTR 45.0%
AAATTTCAGCTCCTCTCCCA+TGG - chr8_1:43745329-43745348 None:intergenic 45.0%
AACCCTAATCTCTCCAATGG+TGG - chr8_1:43745378-43745397 None:intergenic 45.0%
GTGAACCCTAATCTCTCCAA+TGG - chr8_1:43745381-43745400 None:intergenic 45.0%
TTCTGTCTATATACGACGGG+AGG + chr8_1:43745978-43745997 Msa1195600:intron 45.0%
TCTGTCTATATACGACGGGA+GGG + chr8_1:43745979-43745998 Msa1195600:intron 45.0%
ATGCAGAGATATCTCCTTGG+AGG + chr8_1:43746956-43746975 Msa1195600:intron 45.0%
!! GCTACATGGTAATGGTGCAT+TGG + chr8_1:43747307-43747326 Msa1195600:intron 45.0%
TCTCATCCGTTACAGTGTAG+AGG - chr8_1:43747367-43747386 None:intergenic 45.0%
TCATCTTCCGAAATCTCGTC+CGG - chr8_1:43747686-43747705 None:intergenic 45.0%
TGAGGATGAGGATGAAGATG+TGG + chr8_1:43747881-43747900 Msa1195600:three_prime_UTR 45.0%
GAGGATGAGGATGAAGATGT+GGG + chr8_1:43747882-43747901 Msa1195600:three_prime_UTR 45.0%
!! TGGGTAATGAGCATTGAAGG+TGG + chr8_1:43747901-43747920 Msa1195600:three_prime_UTR 45.0%
! TTTTACCGGTGAAGCCATCT+GGG + chr8_1:43747924-43747943 Msa1195600:three_prime_UTR 45.0%
TGATTGTGGGTATGGGGTGA+AGG - chr8_1:43745144-43745163 None:intergenic 50.0%
CTGTCTATATACGACGGGAG+GGG + chr8_1:43745980-43745999 Msa1195600:intron 50.0%
!! GTAAGCAGGGCACTGTTTAC+AGG + chr8_1:43746012-43746031 Msa1195600:intron 50.0%
TGGGAGCGAGCAATTTCTGT+CGG - chr8_1:43746371-43746390 None:intergenic 50.0%
TGCCTGAGTTAACTCACGCA+GGG - chr8_1:43746413-43746432 None:intergenic 50.0%
TTGCCTGAGTTAACTCACGC+AGG - chr8_1:43746414-43746433 None:intergenic 50.0%
GGTAATGCACCACACCTCAA+AGG + chr8_1:43746523-43746542 Msa1195600:intron 50.0%
!! CTTCAGTGCCACTTCAGTAG+TGG - chr8_1:43746559-43746578 None:intergenic 50.0%
TATTCCTAATGCCGCTGCAG+AGG + chr8_1:43747022-43747041 Msa1195600:five_prime_UTR 50.0%
ATCAGCTTCAACCTCTGCAG+CGG - chr8_1:43747036-43747055 None:intergenic 50.0%
! GTTTTACCGGTGAAGCCATC+TGG + chr8_1:43747923-43747942 Msa1195600:three_prime_UTR 50.0%
GAATACCCAGATGGCTTCAC+CGG - chr8_1:43747932-43747951 None:intergenic 50.0%
ATGGGGTGAAGGATGGTACC+AGG - chr8_1:43745133-43745152 None:intergenic 55.0%
TGTGGGTATGGGGTGAAGGA+TGG - chr8_1:43745140-43745159 None:intergenic 55.0%
AAGAGAGGCCGACCAAGATG+AGG + chr8_1:43745297-43745316 Msa1195600:five_prime_UTR 55.0%
GGGAGCGAGCAATTTCTGTC+GGG - chr8_1:43746370-43746389 None:intergenic 55.0%
GCCTGAGTTAACTCACGCAG+GGG - chr8_1:43746412-43746431 None:intergenic 55.0%
TCAACCTCTGCAGCGGCATT+AGG - chr8_1:43747029-43747048 None:intergenic 55.0%
AGGGGTAAGCGAGGGTAAGC+AGG + chr8_1:43745998-43746017 Msa1195600:intron 60.0%
GGGGTAAGCGAGGGTAAGCA+GGG + chr8_1:43745999-43746018 Msa1195600:intron 60.0%
GCCCCTGCGTGAGTTAACTC+AGG + chr8_1:43746408-43746427 Msa1195600:intron 60.0%
TACGACGGGAGGGGTAAGCG+AGG + chr8_1:43745989-43746008 Msa1195600:intron 65.0%
ACGACGGGAGGGGTAAGCGA+GGG + chr8_1:43745990-43746009 Msa1195600:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 43745091 43747971 43745091 ID=Msa1195600;Name=Msa1195600
chr8_1 mRNA 43745091 43747971 43745091 ID=Msa1195600-mRNA-1;Parent=Msa1195600;Name=Msa1195600-mRNA-1;_AED=0.11;_eAED=0.11;_QI=641|1|1|1|0.75|0.8|5|97|67
chr8_1 exon 43745091 43745454 43745091 ID=Msa1195600-mRNA-1:exon:14127;Parent=Msa1195600-mRNA-1
chr8_1 exon 43746962 43747077 43746962 ID=Msa1195600-mRNA-1:exon:14128;Parent=Msa1195600-mRNA-1
chr8_1 exon 43747348 43747423 43747348 ID=Msa1195600-mRNA-1:exon:14129;Parent=Msa1195600-mRNA-1
chr8_1 exon 43747503 43747573 43747503 ID=Msa1195600-mRNA-1:exon:14130;Parent=Msa1195600-mRNA-1
chr8_1 exon 43747657 43747971 43747657 ID=Msa1195600-mRNA-1:exon:14131;Parent=Msa1195600-mRNA-1
chr8_1 five_prime_UTR 43745091 43745454 43745091 ID=Msa1195600-mRNA-1:five_prime_utr;Parent=Msa1195600-mRNA-1
chr8_1 five_prime_UTR 43746962 43747077 43746962 ID=Msa1195600-mRNA-1:five_prime_utr;Parent=Msa1195600-mRNA-1
chr8_1 five_prime_UTR 43747348 43747423 43747348 ID=Msa1195600-mRNA-1:five_prime_utr;Parent=Msa1195600-mRNA-1
chr8_1 five_prime_UTR 43747503 43747573 43747503 ID=Msa1195600-mRNA-1:five_prime_utr;Parent=Msa1195600-mRNA-1
chr8_1 five_prime_UTR 43747657 43747670 43747657 ID=Msa1195600-mRNA-1:five_prime_utr;Parent=Msa1195600-mRNA-1
chr8_1 CDS 43747671 43747874 43747671 ID=Msa1195600-mRNA-1:cds;Parent=Msa1195600-mRNA-1
chr8_1 three_prime_UTR 43747875 43747971 43747875 ID=Msa1195600-mRNA-1:three_prime_utr;Parent=Msa1195600-mRNA-1
Gene Sequence

>Msa1195600

ATGAATGATTATCCGGACGAGATTTCGGAAGATGAAGAAGGTTCTGAGTGTGAATCAGAAGAAAGCAAGCATAGCGGATCTAGTAATGAACTGTCAGATAATGATGAGGATGACGAACTTCGTCGTTTTGCTAAAGGTTTTACTTCTGATCCATTGTATGATGAGGATTTTGATGATTATGAAGGTCAAGGTGTAGGCTTATGA
Protein sequence

>Msa1195600

MNDYPDEISEDEEGSECESEESKHSGSSNELSDNDEDDELRRFAKGFTSDPLYDEDFDDYEGQGVGL*