AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1251260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251260 MtrunA17_Chr8g0366381 98.750 80 1 0 1 80 1 80 2.61e-52 157
Msa1251260 MtrunA17_Chr2g0282641 98.718 78 1 0 1 78 1 78 7.46e-51 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1251260 AT1G74340.1 82.500 80 14 0 1 80 1 80 1.63e-38 123
Msa1251260 AT1G74340.2 82.500 80 14 0 1 80 1 80 1.63e-38 123

Find 48 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGAATCCTCATACATTA+AGG 0.174972 8_2:-47067977 None:intergenic
TATTCACTTATTATACTTTC+TGG 0.197235 8_2:+47070586 Msa1251260:CDS
TGCCTCTTATGTATATTTAT+TGG 0.200273 8_2:+47070800 Msa1251260:CDS
ACATTATGCCTTCTTCTTCT+TGG 0.225894 8_2:-47070840 None:intergenic
GCCTCTTATGTATATTTATT+GGG 0.258483 8_2:+47070801 Msa1251260:CDS
AACAGGAATGAGAAAATTAA+AGG 0.283008 8_2:-47071082 None:intergenic
TATTAATATGGCATATTCTT+GGG 0.311096 8_2:-47070750 None:intergenic
TGCTTTGCCCTTAATGTATG+AGG 0.342292 8_2:+47067969 Msa1251260:five_prime_UTR
GTATTAATATGGCATATTCT+TGG 0.347420 8_2:-47070751 None:intergenic
AGCAGAAACAGGTATTAATA+TGG 0.371224 8_2:-47070762 None:intergenic
TCTGAATCCTCATACATTAA+GGG 0.376447 8_2:-47067976 None:intergenic
TACAGCACACAACATGGATT+TGG 0.394541 8_2:+47071044 Msa1251260:three_prime_UTR
GTGCTGTAGATACTACATTA+AGG 0.398103 8_2:-47071029 None:intergenic
TACTTTCTGGGTTATCATCC+TGG 0.412601 8_2:+47070599 Msa1251260:CDS
ATTCACTTATTATACTTTCT+GGG 0.420687 8_2:+47070587 Msa1251260:CDS
ACAGGAATGAGAAAATTAAA+GGG 0.422501 8_2:-47071081 None:intergenic
ACCCAATAAATATACATAAG+AGG 0.435006 8_2:-47070802 None:intergenic
ATGACCCTAGTGTCAGGAAT+TGG 0.461237 8_2:+47070961 Msa1251260:three_prime_UTR
CTCCTTACATGAGGGTGAAA+AGG 0.469962 8_2:+47071194 Msa1251260:three_prime_UTR
AGAGCAACACCAGCAGAAAC+AGG 0.472824 8_2:-47070773 None:intergenic
ATATTAATACCTGTTTCTGC+TGG 0.492249 8_2:+47070764 Msa1251260:CDS
GAATATCGATAAGCTTATAC+AGG 0.500143 8_2:-47070568 None:intergenic
GAACTAGCAGACAGAGCAGT+TGG 0.505593 8_2:+47070534 Msa1251260:CDS
ACAATGTTTCTTACCGTTCA+CGG 0.507063 8_2:-47068100 None:intergenic
TGAGAAAATTAAAGGGGAAA+CGG 0.510727 8_2:-47071074 None:intergenic
AAATGATCATCATCTACAAA+GGG 0.512690 8_2:-47070707 None:intergenic
CTATGTATGACCCTAGTGTC+AGG 0.521657 8_2:+47070955 Msa1251260:three_prime_UTR
GATCTAAATCTCACATATAA+CGG 0.526160 8_2:+47071221 Msa1251260:three_prime_UTR
TGTGTCCGTCTCCTTACATG+AGG 0.551549 8_2:+47071185 Msa1251260:three_prime_UTR
ATTAATATGGCATATTCTTG+GGG 0.556687 8_2:-47070749 None:intergenic
TTATCGTCGGCCGCCGTGAA+CGG 0.560575 8_2:+47068087 Msa1251260:five_prime_UTR
TATGAGGATTCAGAAAACAT+TGG 0.575208 8_2:+47067985 Msa1251260:five_prime_UTR
GCTCAAATCCAAGAAGAAGA+AGG 0.579376 8_2:+47070832 Msa1251260:CDS
CTTTATACGATCGGTTGAGA+AGG 0.582271 8_2:-47068035 None:intergenic
GGTAGCCAATTCCTGACACT+AGG 0.582684 8_2:-47070966 None:intergenic
GAAATGATCATCATCTACAA+AGG 0.583886 8_2:-47070708 None:intergenic
TTCACCCTCATGTAAGGAGA+CGG 0.586185 8_2:-47071190 None:intergenic
AAGTTCGCCGGAAAAGCCAC+CGG 0.601522 8_2:+47068061 Msa1251260:five_prime_UTR
GTGTCCGTCTCCTTACATGA+GGG 0.606376 8_2:+47071186 Msa1251260:three_prime_UTR
AAGCCACCGGTGTTTATCGT+CGG 0.620208 8_2:+47068074 Msa1251260:five_prime_UTR
AGTATCTACAGCACACAACA+TGG 0.623838 8_2:+47071038 Msa1251260:three_prime_UTR
GTAGCCAATTCCTGACACTA+GGG 0.624009 8_2:-47070965 None:intergenic
CAGGAATGAGAAAATTAAAG+GGG 0.639528 8_2:-47071080 None:intergenic
TACATGAGGGTGAAAAGGAG+AGG 0.641050 8_2:+47071199 Msa1251260:three_prime_UTR
AAATTAAAGGGGAAACGGAG+AGG 0.683197 8_2:-47071069 None:intergenic
CGGCCGACGATAAACACCGG+TGG 0.692824 8_2:-47068077 None:intergenic
CGGCGGCCGACGATAAACAC+CGG 0.701996 8_2:-47068080 None:intergenic
ATGTTTCTTACCGTTCACGG+CGG 0.742764 8_2:-47068097 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAAAGTTATCTAT+TGG - chr8_2:47068175-47068194 None:intergenic 10.0%
!! AACTAAATAGTAATATTATA+CGG + chr8_2:47068506-47068525 Msa1251260:intron 10.0%
!!! TTTTAGTGAGAATTTAAAAT+TGG + chr8_2:47068140-47068159 Msa1251260:intron 15.0%
!!! ATTTTGTATTTTTATTTGGA+TGG - chr8_2:47068856-47068875 None:intergenic 15.0%
!! AATAATAAAACAATGTGAAA+AGG + chr8_2:47068878-47068897 Msa1251260:intron 15.0%
!! ATAAAACTAATAGAAAACAA+GGG + chr8_2:47069872-47069891 Msa1251260:intron 15.0%
!!! ATTTGATGTAGATTTTTATA+AGG + chr8_2:47070332-47070351 Msa1251260:intron 15.0%
!!! CATAATACATTTAAAATACT+TGG - chr8_2:47070636-47070655 None:intergenic 15.0%
!!! CAAGTATTTTAAATGTATTA+TGG + chr8_2:47070634-47070653 Msa1251260:intron 15.0%
!! ATTGAATAAACAAAAATGTT+TGG - chr8_2:47071249-47071268 None:intergenic 15.0%
!!! GAGAATTTAAAATTGGTTTA+TGG + chr8_2:47068147-47068166 Msa1251260:intron 20.0%
!!! AGTGGATTCAATTATATTAT+TGG + chr8_2:47068223-47068242 Msa1251260:intron 20.0%
!! AATTCAATGTTTGAGTAATA+TGG + chr8_2:47068262-47068281 Msa1251260:intron 20.0%
!! ATTCAATGTTTGAGTAATAT+GGG + chr8_2:47068263-47068282 Msa1251260:intron 20.0%
!! AAAGAAAAACAGTACAATTA+AGG - chr8_2:47068567-47068586 None:intergenic 20.0%
!!! TGAAAAGGATGATTTTATAA+AGG + chr8_2:47068893-47068912 Msa1251260:intron 20.0%
!!! GAAAAGGATGATTTTATAAA+GGG + chr8_2:47068894-47068913 Msa1251260:intron 20.0%
!! TTATGAACTTATAATGATGT+TGG + chr8_2:47068984-47069003 Msa1251260:intron 20.0%
!! TACATATACTTAACAAAAAC+AGG - chr8_2:47069102-47069121 None:intergenic 20.0%
!! AAAGATAAAAACTAATGTCT+AGG - chr8_2:47069325-47069344 None:intergenic 20.0%
!!! GAAAATTCAAAAAACCATTT+TGG + chr8_2:47069381-47069400 Msa1251260:intron 20.0%
!!! TTACTATGAGGTAATATTTT+AGG - chr8_2:47069805-47069824 None:intergenic 20.0%
!!! TTTTCTATTAGTTTTATCGA+AGG - chr8_2:47069869-47069888 None:intergenic 20.0%
!! GATAAAACTAATAGAAAACA+AGG + chr8_2:47069871-47069890 Msa1251260:intron 20.0%
!! TTCAAAAAATCATTAGAGTT+CGG + chr8_2:47070143-47070162 Msa1251260:intron 20.0%
!! TATTCACTTATTATACTTTC+TGG + chr8_2:47070586-47070605 Msa1251260:CDS 20.0%
!! ATTCACTTATTATACTTTCT+GGG + chr8_2:47070587-47070606 Msa1251260:CDS 20.0%
!! CTTATTAGGAAGTTAAAATA+AGG - chr8_2:47070663-47070682 None:intergenic 20.0%
!! ACAATATCGAAAAACTTATT+AGG - chr8_2:47070677-47070696 None:intergenic 20.0%
!! TATTAATATGGCATATTCTT+GGG - chr8_2:47070753-47070772 None:intergenic 20.0%
!!! ACCATTTTATACATGAAAAA+TGG + chr8_2:47070924-47070943 Msa1251260:three_prime_UTR 20.0%
!!! TCCATTTTTCATGTATAAAA+TGG - chr8_2:47070928-47070947 None:intergenic 20.0%
!!! CTCAATTTTGTTTGTTTATA+AGG + chr8_2:47071137-47071156 Msa1251260:three_prime_UTR 20.0%
!!! TTTGATTTCACTTTAAAAAG+TGG - chr8_2:47071283-47071302 None:intergenic 20.0%
!!! AATTTTAAGTGTTGATGGAT+TGG - chr8_2:47067947-47067966 None:intergenic 25.0%
!!! GAACTTTTTCTTTATACGAT+CGG - chr8_2:47068047-47068066 None:intergenic 25.0%
!! ATTGATTGATCTTTTCCTTT+TGG + chr8_2:47068192-47068211 Msa1251260:intron 25.0%
! GTACAAAATAGTAACATTTC+CGG - chr8_2:47068485-47068504 None:intergenic 25.0%
!!! ATATATTCCTTTCTAGAATC+CGG + chr8_2:47068612-47068631 Msa1251260:intron 25.0%
! ATGGTGGCTATATATAATAT+GGG - chr8_2:47069230-47069249 None:intergenic 25.0%
! AATGGTGGCTATATATAATA+TGG - chr8_2:47069231-47069250 None:intergenic 25.0%
!! GATTTTGTAGTGTACAATAT+TGG - chr8_2:47069906-47069925 None:intergenic 25.0%
!!! CTTGATTTTCTGCTTATTTT+TGG + chr8_2:47069967-47069986 Msa1251260:intron 25.0%
!! AAATTGGTTTCTAAACATGT+TGG - chr8_2:47070057-47070076 None:intergenic 25.0%
! ACCAATTTGACAAATCAATT+TGG + chr8_2:47070069-47070088 Msa1251260:intron 25.0%
!!! TCCAAATTGATTTGTCAAAT+TGG - chr8_2:47070073-47070092 None:intergenic 25.0%
!!! AATGATTTTTTGAATCGTTG+AGG - chr8_2:47070137-47070156 None:intergenic 25.0%
!! GATGTAGATTTTTATAAGGA+AGG + chr8_2:47070336-47070355 Msa1251260:intron 25.0%
!! CAAAATATTGATAGCATACT+CGG - chr8_2:47070420-47070439 None:intergenic 25.0%
! AAAAACGATAGGAATCAAAA+TGG + chr8_2:47070512-47070531 Msa1251260:exon 25.0%
! AAATGATCATCATCTACAAA+GGG - chr8_2:47070710-47070729 None:intergenic 25.0%
! ATTAATATGGCATATTCTTG+GGG - chr8_2:47070752-47070771 None:intergenic 25.0%
! GTATTAATATGGCATATTCT+TGG - chr8_2:47070754-47070773 None:intergenic 25.0%
! TGCCTCTTATGTATATTTAT+TGG + chr8_2:47070800-47070819 Msa1251260:CDS 25.0%
! GCCTCTTATGTATATTTATT+GGG + chr8_2:47070801-47070820 Msa1251260:CDS 25.0%
! ACCCAATAAATATACATAAG+AGG - chr8_2:47070805-47070824 None:intergenic 25.0%
! ACAGGAATGAGAAAATTAAA+GGG - chr8_2:47071084-47071103 None:intergenic 25.0%
! AACAGGAATGAGAAAATTAA+AGG - chr8_2:47071085-47071104 None:intergenic 25.0%
! GATCTAAATCTCACATATAA+CGG + chr8_2:47071221-47071240 Msa1251260:three_prime_UTR 25.0%
!! CACTTTAAAAAGTGGTAATA+AGG - chr8_2:47071275-47071294 None:intergenic 25.0%
!!! GCAGTAATTTTAAGTGTTGA+TGG - chr8_2:47067952-47067971 None:intergenic 30.0%
TCTGAATCCTCATACATTAA+GGG - chr8_2:47067979-47067998 None:intergenic 30.0%
TTCTGAATCCTCATACATTA+AGG - chr8_2:47067980-47067999 None:intergenic 30.0%
TATGAGGATTCAGAAAACAT+TGG + chr8_2:47067985-47068004 Msa1251260:five_prime_UTR 30.0%
GTGATTCGATCTAAAAAAGA+GGG - chr8_2:47068463-47068482 None:intergenic 30.0%
!!! TCTTTTTTAGATCGAATCAC+CGG + chr8_2:47068463-47068482 Msa1251260:intron 30.0%
TACAATTAAGGACGTTATTC+AGG - chr8_2:47068555-47068574 None:intergenic 30.0%
! GGATTCTAGAAAGGAATATA+TGG - chr8_2:47068613-47068632 None:intergenic 30.0%
ACAGGAAAATTCAACATAGA+CGG - chr8_2:47068657-47068676 None:intergenic 30.0%
! CTTCTTTTAACCGAAGAATA+AGG + chr8_2:47068723-47068742 Msa1251260:intron 30.0%
CAACTAAAAGAACTAGATGA+AGG + chr8_2:47068751-47068770 Msa1251260:intron 30.0%
!! TGAGAAAATTTTGATGGAGA+GGG + chr8_2:47068807-47068826 Msa1251260:intron 30.0%
! AGGATGATTTTATAAAGGGT+TGG + chr8_2:47068898-47068917 Msa1251260:intron 30.0%
TTGCATGCTAAGATGATTAA+GGG - chr8_2:47069039-47069058 None:intergenic 30.0%
ATTAGGAAAAGGATGGTTAT+TGG + chr8_2:47069060-47069079 Msa1251260:intron 30.0%
TTAGGAAAAGGATGGTTATT+GGG + chr8_2:47069061-47069080 Msa1251260:intron 30.0%
CTTCTATGTCTTTCTTCAAT+AGG + chr8_2:47069167-47069186 Msa1251260:intron 30.0%
TGGTGGCTATATATAATATG+GGG - chr8_2:47069229-47069248 None:intergenic 30.0%
TTGACATGAATGAACCAAAA+TGG - chr8_2:47069398-47069417 None:intergenic 30.0%
AATATCTGTAGCAAATGAGA+TGG + chr8_2:47069445-47069464 Msa1251260:intron 30.0%
ATGAAGTCAAATTGAGTAGA+AGG - chr8_2:47069602-47069621 None:intergenic 30.0%
TCCTCTTCACAAAAATACAA+AGG + chr8_2:47069829-47069848 Msa1251260:intron 30.0%
!! ACCTTTGTATTTTTGTGAAG+AGG - chr8_2:47069833-47069852 None:intergenic 30.0%
ATGAATCAATTGTCTAGGTT+CGG - chr8_2:47069929-47069948 None:intergenic 30.0%
TTTAAATTCCCAAACAGAAC+TGG - chr8_2:47070110-47070129 None:intergenic 30.0%
TTTCTTTGTTAACTCTGTCA+TGG + chr8_2:47070233-47070252 Msa1251260:intron 30.0%
CATCAAATTGATGAATGTGA+AGG - chr8_2:47070320-47070339 None:intergenic 30.0%
GAATATCGATAAGCTTATAC+AGG - chr8_2:47070571-47070590 None:intergenic 30.0%
GAAATGATCATCATCTACAA+AGG - chr8_2:47070711-47070730 None:intergenic 30.0%
AGCAGAAACAGGTATTAATA+TGG - chr8_2:47070765-47070784 None:intergenic 30.0%
!! ATATTAATACCTGTTTCTGC+TGG + chr8_2:47070764-47070783 Msa1251260:CDS 30.0%
TGAGAAAATTAAAGGGGAAA+CGG - chr8_2:47071077-47071096 None:intergenic 30.0%
CAGGAATGAGAAAATTAAAG+GGG - chr8_2:47071083-47071102 None:intergenic 30.0%
CGTATAAAGAAAAAGTTCGC+CGG + chr8_2:47068049-47068068 Msa1251260:five_prime_UTR 35.0%
ACAATGTTTCTTACCGTTCA+CGG - chr8_2:47068103-47068122 None:intergenic 35.0%
!!! TTCCTTTTGGTTAAGTTGAG+TGG + chr8_2:47068205-47068224 Msa1251260:intron 35.0%
ATCCACTCAACTTAACCAAA+AGG - chr8_2:47068210-47068229 None:intergenic 35.0%
AGTCAAGAATGATTCGAATG+AGG + chr8_2:47068391-47068410 Msa1251260:intron 35.0%
GTCAAGAATGATTCGAATGA+GGG + chr8_2:47068392-47068411 Msa1251260:intron 35.0%
GGTGATTCGATCTAAAAAAG+AGG - chr8_2:47068464-47068483 None:intergenic 35.0%
CGTTATTCAGGACAAAGAAA+AGG - chr8_2:47068543-47068562 None:intergenic 35.0%
AGGAAAATTCAACATAGACG+GGG - chr8_2:47068655-47068674 None:intergenic 35.0%
CAGGAAAATTCAACATAGAC+GGG - chr8_2:47068656-47068675 None:intergenic 35.0%
GAAATTGGGAGTGAATACTT+GGG + chr8_2:47068687-47068706 Msa1251260:intron 35.0%
AAATTGGGAGTGAATACTTG+GGG + chr8_2:47068688-47068707 Msa1251260:intron 35.0%
AGTTGCTACACCTTATTCTT+CGG - chr8_2:47068736-47068755 None:intergenic 35.0%
!! ACGGACTGAGAAAATTTTGA+TGG + chr8_2:47068801-47068820 Msa1251260:intron 35.0%
!! CTGAGAAAATTTTGATGGAG+AGG + chr8_2:47068806-47068825 Msa1251260:intron 35.0%
TGCATGCTAAGATGATTAAG+GGG - chr8_2:47069038-47069057 None:intergenic 35.0%
GTTGCATGCTAAGATGATTA+AGG - chr8_2:47069040-47069059 None:intergenic 35.0%
TCATCTTAGCATGCAACATT+AGG + chr8_2:47069043-47069062 Msa1251260:intron 35.0%
TAGCATGCAACATTAGGAAA+AGG + chr8_2:47069049-47069068 Msa1251260:intron 35.0%
ATGCAACATTAGGAAAAGGA+TGG + chr8_2:47069053-47069072 Msa1251260:intron 35.0%
! ACCATTCACACTAGTTTGAT+GGG + chr8_2:47069245-47069264 Msa1251260:intron 35.0%
ACTTCAGTATAAACTGAAGC+AGG - chr8_2:47069273-47069292 None:intergenic 35.0%
CTAATGTCTAGGTGAAAGAT+TGG - chr8_2:47069314-47069333 None:intergenic 35.0%
GGCTAAGACAATGACAAATT+GGG - chr8_2:47069474-47069493 None:intergenic 35.0%
CAATTTGTCATTGTCTTAGC+CGG + chr8_2:47069473-47069492 Msa1251260:intron 35.0%
ATCACATTATCACTAGCTAC+TGG - chr8_2:47069535-47069554 None:intergenic 35.0%
AATTCCTATCAAGTTCACTC+TGG - chr8_2:47069564-47069583 None:intergenic 35.0%
! ATTTGACTTCATCCAATGCT+TGG + chr8_2:47069610-47069629 Msa1251260:intron 35.0%
GAAGAGGAACTATTACTATG+AGG - chr8_2:47069817-47069836 None:intergenic 35.0%
TGAATCAATTGTCTAGGTTC+GGG - chr8_2:47069928-47069947 None:intergenic 35.0%
AGGGTATGAATCAATTGTCT+AGG - chr8_2:47069934-47069953 None:intergenic 35.0%
! GATTCATACCCTCATTTTCA+TGG + chr8_2:47069942-47069961 Msa1251260:intron 35.0%
ATCAAGCACCATGAAAATGA+GGG - chr8_2:47069953-47069972 None:intergenic 35.0%
AATCAAGCACCATGAAAATG+AGG - chr8_2:47069954-47069973 None:intergenic 35.0%
!!! TATTTTTGGAGCCTGTTAAC+AGG + chr8_2:47069981-47070000 Msa1251260:intron 35.0%
!!! ATTTTTGGAGCCTGTTAACA+GGG + chr8_2:47069982-47070001 Msa1251260:intron 35.0%
TGGTAGAAGTTAAGAACCAA+AGG + chr8_2:47070009-47070028 Msa1251260:intron 35.0%
TCAAAAACTGCTTGATCCTT+TGG - chr8_2:47070028-47070047 None:intergenic 35.0%
TTCAGAAACCAGTTCTGTTT+GGG + chr8_2:47070099-47070118 Msa1251260:intron 35.0%
GTACCAAAAGTGGTTAAGTT+TGG + chr8_2:47070174-47070193 Msa1251260:intron 35.0%
! GAACCAAACTTAACCACTTT+TGG - chr8_2:47070180-47070199 None:intergenic 35.0%
! TAAGTTTGGTTCAACTGAAC+TGG + chr8_2:47070188-47070207 Msa1251260:intron 35.0%
!! TGTGTATTTTTGTTGTCGAC+AGG + chr8_2:47070485-47070504 Msa1251260:intron 35.0%
ACATTATGCCTTCTTCTTCT+TGG - chr8_2:47070843-47070862 None:intergenic 35.0%
GTGCTGTAGATACTACATTA+AGG - chr8_2:47071032-47071051 None:intergenic 35.0%
! GTCTCTATCTCTTTTCTAAC+AGG - chr8_2:47071102-47071121 None:intergenic 35.0%
TGCTTTGCCCTTAATGTATG+AGG + chr8_2:47067969-47067988 Msa1251260:five_prime_UTR 40.0%
CTTTATACGATCGGTTGAGA+AGG - chr8_2:47068038-47068057 None:intergenic 40.0%
! TACTTGTCCGGATTCTAGAA+AGG - chr8_2:47068622-47068641 None:intergenic 40.0%
GGTAAGTTCAGTTACTTGTC+CGG - chr8_2:47068634-47068653 None:intergenic 40.0%
CGAAATTGGGAGTGAATACT+TGG + chr8_2:47068686-47068705 Msa1251260:intron 40.0%
AATACTTGGGGTTGAAGCTA+CGG + chr8_2:47068700-47068719 Msa1251260:intron 40.0%
ACTAGATGAAGGCAAAGAGA+TGG + chr8_2:47068762-47068781 Msa1251260:intron 40.0%
CTAGATGAAGGCAAAGAGAT+GGG + chr8_2:47068763-47068782 Msa1251260:intron 40.0%
CATCAAACTAGTGTGAATGG+TGG - chr8_2:47069246-47069265 None:intergenic 40.0%
! CACCATTCACACTAGTTTGA+TGG + chr8_2:47069244-47069263 Msa1251260:intron 40.0%
! AACTCTTGCCTCGCTTTAAA+TGG + chr8_2:47069355-47069374 Msa1251260:intron 40.0%
! ACTCTTGCCTCGCTTTAAAT+GGG + chr8_2:47069356-47069375 Msa1251260:intron 40.0%
!! TTTTCGACCCATTTAAAGCG+AGG - chr8_2:47069366-47069385 None:intergenic 40.0%
CGGCTAAGACAATGACAAAT+TGG - chr8_2:47069475-47069494 None:intergenic 40.0%
TCAACATTGACGACACAAGT+TGG - chr8_2:47069502-47069521 None:intergenic 40.0%
TGTCGTCAATGTTGATCAGA+CGG + chr8_2:47069507-47069526 Msa1251260:intron 40.0%
AAGTAAGCAAGTCCAAGCAT+TGG - chr8_2:47069625-47069644 None:intergenic 40.0%
! TGCTTGGACTTGCTTACTTT+AGG + chr8_2:47069626-47069645 Msa1251260:intron 40.0%
GCACAAGACAAAGAATCTTG+TGG - chr8_2:47069743-47069762 None:intergenic 40.0%
GATTCTTTGTCTTGTGCAGA+TGG + chr8_2:47069746-47069765 Msa1251260:intron 40.0%
CTACCAATTTCCCTGTTAAC+AGG - chr8_2:47069995-47070014 None:intergenic 40.0%
GTTCAGAAACCAGTTCTGTT+TGG + chr8_2:47070098-47070117 Msa1251260:intron 40.0%
AATCATTAGAGTTCGGTCCA+TGG + chr8_2:47070150-47070169 Msa1251260:intron 40.0%
!! TAACCACTTTTGGTACTCCA+TGG - chr8_2:47070170-47070189 None:intergenic 40.0%
TGCAATGAGCTGAAAGGAAT+TGG - chr8_2:47070376-47070395 None:intergenic 40.0%
AAAGTCTGCAATGAGCTGAA+AGG - chr8_2:47070382-47070401 None:intergenic 40.0%
TACTTTCTGGGTTATCATCC+TGG + chr8_2:47070599-47070618 Msa1251260:CDS 40.0%
TACTTGGCATGTTACTAACC+AGG - chr8_2:47070620-47070639 None:intergenic 40.0%
GCTCAAATCCAAGAAGAAGA+AGG + chr8_2:47070832-47070851 Msa1251260:CDS 40.0%
GCTACAAAAACGAGGTAATG+TGG - chr8_2:47070990-47071009 None:intergenic 40.0%
!! CATTACCTCGTTTTTGTAGC+TGG + chr8_2:47070990-47071009 Msa1251260:three_prime_UTR 40.0%
AGTATCTACAGCACACAACA+TGG + chr8_2:47071038-47071057 Msa1251260:three_prime_UTR 40.0%
TACAGCACACAACATGGATT+TGG + chr8_2:47071044-47071063 Msa1251260:three_prime_UTR 40.0%
AAATTAAAGGGGAAACGGAG+AGG - chr8_2:47071072-47071091 None:intergenic 40.0%
! ATAAACACCGGTGGCTTTTC+CGG - chr8_2:47068071-47068090 None:intergenic 45.0%
ATGTTTCTTACCGTTCACGG+CGG - chr8_2:47068100-47068119 None:intergenic 45.0%
! AATGAGGGAGAACTTTTGCC+CGG + chr8_2:47068407-47068426 Msa1251260:intron 45.0%
TGAAACCTTCCTCCTCAAAC+CGG - chr8_2:47068429-47068448 None:intergenic 45.0%
GGACAAAGAAAAGGATGCAC+AGG - chr8_2:47068534-47068553 None:intergenic 45.0%
CTGTGTCTTGCGTCGAAATT+GGG + chr8_2:47068673-47068692 Msa1251260:intron 45.0%
GGGTGAGTGTGGAAGATAAT+CGG + chr8_2:47068827-47068846 Msa1251260:intron 45.0%
GCCCATCAAACTAGTGTGAA+TGG - chr8_2:47069249-47069268 None:intergenic 45.0%
!! TGACGACACAAGTTGGTTAC+CGG - chr8_2:47069495-47069514 None:intergenic 45.0%
ACCACCAGAGTGAACTTGAT+AGG + chr8_2:47069557-47069576 Msa1251260:intron 45.0%
TCCTATCAAGTTCACTCTGG+TGG - chr8_2:47069561-47069580 None:intergenic 45.0%
GAGCCTGTTAACAGGGAAAT+TGG + chr8_2:47069989-47070008 Msa1251260:intron 45.0%
CGACAGGAAGCAAAAACGAT+AGG + chr8_2:47070501-47070520 Msa1251260:intron 45.0%
CTATGTATGACCCTAGTGTC+AGG + chr8_2:47070955-47070974 Msa1251260:three_prime_UTR 45.0%
ATGACCCTAGTGTCAGGAAT+TGG + chr8_2:47070961-47070980 Msa1251260:three_prime_UTR 45.0%
GTAGCCAATTCCTGACACTA+GGG - chr8_2:47070968-47070987 None:intergenic 45.0%
!! TACCTCGTTTTTGTAGCTGG+CGG + chr8_2:47070993-47071012 Msa1251260:three_prime_UTR 45.0%
TTCACCCTCATGTAAGGAGA+CGG - chr8_2:47071193-47071212 None:intergenic 45.0%
CTCCTTACATGAGGGTGAAA+AGG + chr8_2:47071194-47071213 Msa1251260:three_prime_UTR 45.0%
! CTCCTTTTCACCCTCATGTA+AGG - chr8_2:47071199-47071218 None:intergenic 45.0%
TACATGAGGGTGAAAAGGAG+AGG + chr8_2:47071199-47071218 Msa1251260:three_prime_UTR 45.0%
!!! TTTTATTTTGTATTTTTATT+TGG - chr8_2:47068860-47068879 None:intergenic 5.0%
AAGCCACCGGTGTTTATCGT+CGG + chr8_2:47068074-47068093 Msa1251260:five_prime_UTR 50.0%
!! GAGAACTTTTGCCCGGTTTG+AGG + chr8_2:47068414-47068433 Msa1251260:intron 50.0%
!! AACTTTTGCCCGGTTTGAGG+AGG + chr8_2:47068417-47068436 Msa1251260:intron 50.0%
GAAACCTTCCTCCTCAAACC+GGG - chr8_2:47068428-47068447 None:intergenic 50.0%
CCAATTTCGACGCAAGACAC+AGG - chr8_2:47068675-47068694 None:intergenic 50.0%
CCTGTGTCTTGCGTCGAAAT+TGG + chr8_2:47068672-47068691 Msa1251260:intron 50.0%
! TGGGAGTGTGTGGAAGATGA+CGG + chr8_2:47068782-47068801 Msa1251260:intron 50.0%
TTTGATGGAGAGGGTGAGTG+TGG + chr8_2:47068816-47068835 Msa1251260:intron 50.0%
! GGTCCATGGAGTACCAAAAG+TGG + chr8_2:47070164-47070183 Msa1251260:intron 50.0%
GAACTAGCAGACAGAGCAGT+TGG + chr8_2:47070534-47070553 Msa1251260:CDS 50.0%
AGAGCAACACCAGCAGAAAC+AGG - chr8_2:47070776-47070795 None:intergenic 50.0%
GGTAGCCAATTCCTGACACT+AGG - chr8_2:47070969-47070988 None:intergenic 50.0%
!! ACCTCGTTTTTGTAGCTGGC+GGG + chr8_2:47070994-47071013 Msa1251260:three_prime_UTR 50.0%
TGTGTCCGTCTCCTTACATG+AGG + chr8_2:47071185-47071204 Msa1251260:three_prime_UTR 50.0%
GTGTCCGTCTCCTTACATGA+GGG + chr8_2:47071186-47071205 Msa1251260:three_prime_UTR 50.0%
AAGTTCGCCGGAAAAGCCAC+CGG + chr8_2:47068061-47068080 Msa1251260:five_prime_UTR 55.0%
! TTTGCCCGGTTTGAGGAGGA+AGG + chr8_2:47068421-47068440 Msa1251260:intron 55.0%
! GGCAAAGAGATGGGAGTGTG+TGG + chr8_2:47068772-47068791 Msa1251260:intron 55.0%
!! CCTCGTTTTTGTAGCTGGCG+GGG + chr8_2:47070995-47071014 Msa1251260:three_prime_UTR 55.0%
TTATCGTCGGCCGCCGTGAA+CGG + chr8_2:47068087-47068106 Msa1251260:five_prime_UTR 60.0%
CCCCGCCAGCTACAAAAACG+AGG - chr8_2:47070998-47071017 None:intergenic 60.0%
CGGCCGACGATAAACACCGG+TGG - chr8_2:47068080-47068099 None:intergenic 65.0%
CGGCGGCCGACGATAAACAC+CGG - chr8_2:47068083-47068102 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 47067921 47071316 47067921 ID=Msa1251260;Name=Msa1251260
chr8_2 mRNA 47067921 47071316 47067921 ID=Msa1251260-mRNA-1;Parent=Msa1251260;Name=Msa1251260-mRNA-1;_AED=0.01;_eAED=0.01;_QI=212|1|1|1|0.5|0.66|3|457|80
chr8_2 exon 47067921 47068108 47067921 ID=Msa1251260-mRNA-1:exon:14257;Parent=Msa1251260-mRNA-1
chr8_2 exon 47070507 47070620 47070507 ID=Msa1251260-mRNA-1:exon:14258;Parent=Msa1251260-mRNA-1
chr8_2 exon 47070707 47071316 47070707 ID=Msa1251260-mRNA-1:exon:14259;Parent=Msa1251260-mRNA-1
chr8_2 five_prime_UTR 47067921 47068108 47067921 ID=Msa1251260-mRNA-1:five_prime_utr;Parent=Msa1251260-mRNA-1
chr8_2 five_prime_UTR 47070507 47070530 47070507 ID=Msa1251260-mRNA-1:five_prime_utr;Parent=Msa1251260-mRNA-1
chr8_2 CDS 47070531 47070620 47070531 ID=Msa1251260-mRNA-1:cds;Parent=Msa1251260-mRNA-1
chr8_2 CDS 47070707 47070859 47070707 ID=Msa1251260-mRNA-1:cds;Parent=Msa1251260-mRNA-1
chr8_2 three_prime_UTR 47070860 47071316 47070860 ID=Msa1251260-mRNA-1:three_prime_utr;Parent=Msa1251260-mRNA-1
Gene Sequence

>Msa1251260

ATGGAACTAGCAGACAGAGCAGTTGGATTTCTATTGTCCTGTATAAGCTTATCGATATTCACTTATTATACTTTCTGGGTTATCATCCTGCCCTTTGTAGATGATGATCATTTCGTACACAAGTATTTCTTACCCCAAGAATATGCCATATTAATACCTGTTTCTGCTGGTGTTGCTCTTATTTGCCTCTTATGTATATTTATTGGGTTCGTGATGCTCAAATCCAAGAAGAAGAAGGCATAA
Protein sequence

>Msa1251260

MELADRAVGFLLSCISLSIFTYYTFWVIILPFVDDDHFVHKYFLPQEYAILIPVSAGVALICLLCIFIGFVMLKSKKKKA*