AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1291910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291910 MtrunA17_Chr8g0366381 98.750 80 1 0 1 80 1 80 2.61e-52 157
Msa1291910 MtrunA17_Chr2g0282641 98.718 78 1 0 1 78 1 78 7.46e-51 154
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1291910 AT1G74340.1 82.500 80 14 0 1 80 1 80 1.63e-38 123
Msa1291910 AT1G74340.2 82.500 80 14 0 1 80 1 80 1.63e-38 123

Find 48 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGAATCCTCATACATTA+AGG 0.174972 8_3:+47493218 None:intergenic
TATTCACTTATTATACTTTC+TGG 0.197235 8_3:-47490563 Msa1291910:CDS
TGCCTCTTATGTATATTTAT+TGG 0.200273 8_3:-47490349 Msa1291910:CDS
ACATTATGCCTTCTTCTTCT+TGG 0.225894 8_3:+47490309 None:intergenic
AACAGGAATGAGAAAATTAA+AGG 0.253882 8_3:+47490068 None:intergenic
GCCTCTTATGTATATTTATT+GGG 0.258483 8_3:-47490348 Msa1291910:CDS
TATTAATATGGCATATTCTT+GGG 0.311096 8_3:+47490399 None:intergenic
TGCTTTGCCCTTAATGTATG+AGG 0.342292 8_3:-47493226 Msa1291910:five_prime_UTR
GTATTAATATGGCATATTCT+TGG 0.347420 8_3:+47490398 None:intergenic
AGCAGAAACAGGTATTAATA+TGG 0.371224 8_3:+47490387 None:intergenic
TCTGAATCCTCATACATTAA+GGG 0.376447 8_3:+47493219 None:intergenic
TACAGCACACAACATGGATT+TGG 0.394541 8_3:-47490106 Msa1291910:three_prime_UTR
GTGCTGTAGATACTACATTA+AGG 0.398103 8_3:+47490121 None:intergenic
TACTTTCTGGGTTATCATCC+TGG 0.412601 8_3:-47490550 Msa1291910:intron
ATTCACTTATTATACTTTCT+GGG 0.420687 8_3:-47490562 Msa1291910:CDS
TGAGAAAATTAAAGGGAAAA+CGG 0.420864 8_3:+47490076 None:intergenic
ACCCAATAAATATACATAAG+AGG 0.435006 8_3:+47490347 None:intergenic
ATGACCCTAGTGTCAGGAAT+TGG 0.461237 8_3:-47490189 Msa1291910:three_prime_UTR
CTCCTTACATGAGGGTGAAA+AGG 0.469962 8_3:-47489956 Msa1291910:three_prime_UTR
AGAGCAACACCAGCAGAAAC+AGG 0.472824 8_3:+47490376 None:intergenic
ACAGGAATGAGAAAATTAAA+GGG 0.475144 8_3:+47490069 None:intergenic
ATATTAATACCTGTTTCTGC+TGG 0.492249 8_3:-47490385 Msa1291910:CDS
GAATATCGATAAGCTTATAC+AGG 0.500143 8_3:+47490581 None:intergenic
GAACTAGCAGACAGAGCAGT+TGG 0.505593 8_3:-47490615 Msa1291910:CDS
ACAATGTTTCTTACCGTTCA+CGG 0.507063 8_3:+47493091 None:intergenic
AAATGATCATCATCTACAAA+GGG 0.512690 8_3:+47490442 None:intergenic
CTATGTATGACCCTAGTGTC+AGG 0.521657 8_3:-47490195 Msa1291910:three_prime_UTR
GATCTAAATCTCACATATAA+CGG 0.526160 8_3:-47489929 Msa1291910:three_prime_UTR
TGTGTCCGTCTCCTTACATG+AGG 0.551549 8_3:-47489965 Msa1291910:three_prime_UTR
ATTAATATGGCATATTCTTG+GGG 0.556687 8_3:+47490400 None:intergenic
TTATCGTCGGCCGCCGTGAA+CGG 0.560575 8_3:-47493104 Msa1291910:intron
TATGAGGATTCAGAAAACAT+TGG 0.575208 8_3:-47493210 Msa1291910:five_prime_UTR
GCTCAAATCCAAGAAGAAGA+AGG 0.579376 8_3:-47490317 Msa1291910:CDS
GGTAGCCAATTCCTGACACT+AGG 0.582684 8_3:+47490184 None:intergenic
GAAATGATCATCATCTACAA+AGG 0.583886 8_3:+47490441 None:intergenic
TTCACCCTCATGTAAGGAGA+CGG 0.586185 8_3:+47489960 None:intergenic
AAGTTCGCCGGAAAAGCCAC+CGG 0.601522 8_3:-47493130 Msa1291910:five_prime_UTR
GTGTCCGTCTCCTTACATGA+GGG 0.606376 8_3:-47489964 Msa1291910:three_prime_UTR
ATACGATCGATTGAGAAGGT+AGG 0.609429 8_3:+47493160 None:intergenic
AAGCCACCGGTGTTTATCGT+CGG 0.620208 8_3:-47493117 Msa1291910:five_prime_UTR
AGTATCTACAGCACACAACA+TGG 0.623838 8_3:-47490112 Msa1291910:three_prime_UTR
GTAGCCAATTCCTGACACTA+GGG 0.624009 8_3:+47490185 None:intergenic
CTTTATACGATCGATTGAGA+AGG 0.624541 8_3:+47493156 None:intergenic
TACATGAGGGTGAAAAGGAG+AGG 0.641050 8_3:-47489951 Msa1291910:three_prime_UTR
AAATTAAAGGGAAAACGGAG+AGG 0.692364 8_3:+47490081 None:intergenic
CGGCCGACGATAAACACCGG+TGG 0.692824 8_3:+47493114 None:intergenic
CGGCGGCCGACGATAAACAC+CGG 0.701996 8_3:+47493111 None:intergenic
ATGTTTCTTACCGTTCACGG+CGG 0.742764 8_3:+47493094 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATAAAGTTATCTAT+TGG + chr8_3:47490114-47490133 None:intergenic 10.0%
!! AACTAAATAGTAATATTATA+CGG - chr8_3:47490459-47490478 Msa1291910:intron 10.0%
!!! TTTTAGTGAGAATTTAAAAT+TGG - chr8_3:47490079-47490098 Msa1291910:three_prime_UTR 15.0%
!!! ATTTTGTATTTTTATTTGGA+TGG + chr8_3:47490813-47490832 None:intergenic 15.0%
!! AATAATAAAACAATGTGAAA+AGG - chr8_3:47490835-47490854 Msa1291910:intron 15.0%
!! ATAAAACTAATAGAAAACAA+GGG - chr8_3:47491832-47491851 Msa1291910:intron 15.0%
!!! ATTTGATGTAGATTTTTATA+AGG - chr8_3:47492313-47492332 Msa1291910:intron 15.0%
!!! CATAATACATTTAAAATACT+TGG + chr8_3:47492617-47492636 None:intergenic 15.0%
!!! CAAGTATTTTAAATGTATTA+TGG - chr8_3:47492615-47492634 Msa1291910:intron 15.0%
!! ATTGAATAAACAAAAATGTT+TGG + chr8_3:47493229-47493248 None:intergenic 15.0%
!!! GAGAATTTAAAATTGGTTTA+TGG - chr8_3:47490086-47490105 Msa1291910:three_prime_UTR 20.0%
!!! AGTGGATTCAATTATATTAT+TGG - chr8_3:47490162-47490181 Msa1291910:three_prime_UTR 20.0%
!! AATTCAATGTTTGAGTAATA+TGG - chr8_3:47490201-47490220 Msa1291910:three_prime_UTR 20.0%
!! ATTCAATGTTTGAGTAATAT+GGG - chr8_3:47490202-47490221 Msa1291910:three_prime_UTR 20.0%
!! AAAGAAAAACAGTACAATTA+AGG + chr8_3:47490520-47490539 None:intergenic 20.0%
!!! TGAAAAGGATGATTTTATAA+AGG - chr8_3:47490850-47490869 Msa1291910:intron 20.0%
!!! GAAAAGGATGATTTTATAAA+GGG - chr8_3:47490851-47490870 Msa1291910:intron 20.0%
!! TTATGAACTTATAATGATGT+TGG - chr8_3:47490941-47490960 Msa1291910:intron 20.0%
!! TACATATACTTAACAAAAAC+AGG + chr8_3:47491059-47491078 None:intergenic 20.0%
!! AAAGATAAAAACTAATGTCT+AGG + chr8_3:47491285-47491304 None:intergenic 20.0%
!!! GAAAATTCAAAAAACCATTT+TGG - chr8_3:47491341-47491360 Msa1291910:intron 20.0%
!!! TTACTATGAGGTAATATTTT+AGG + chr8_3:47491765-47491784 None:intergenic 20.0%
!!! TTTTCTATTAGTTTTATCGA+AGG + chr8_3:47491829-47491848 None:intergenic 20.0%
!! GATAAAACTAATAGAAAACA+AGG - chr8_3:47491831-47491850 Msa1291910:intron 20.0%
!! TTCAAAAAATCATTAGAGTT+CGG - chr8_3:47492109-47492128 Msa1291910:intron 20.0%
!! TATTCACTTATTATACTTTC+TGG - chr8_3:47492567-47492586 Msa1291910:intron 20.0%
!! ATTCACTTATTATACTTTCT+GGG - chr8_3:47492568-47492587 Msa1291910:intron 20.0%
!! CTTATTAGGAAGTTAAAATA+AGG + chr8_3:47492644-47492663 None:intergenic 20.0%
!! ACAATATCGAAAAACTTATT+AGG + chr8_3:47492658-47492677 None:intergenic 20.0%
!! TATTAATATGGCATATTCTT+GGG + chr8_3:47492734-47492753 None:intergenic 20.0%
!!! TACCATTTTATACATGAAAA+TGG - chr8_3:47492904-47492923 Msa1291910:intron 20.0%
!!! TTCCATTTTCATGTATAAAA+TGG + chr8_3:47492909-47492928 None:intergenic 20.0%
!!! CTCAATTTTGTTTGTTTATA+AGG - chr8_3:47493117-47493136 Msa1291910:five_prime_UTR 20.0%
!!! TTTGATTTCACTTTAAAAAG+TGG + chr8_3:47493263-47493282 None:intergenic 20.0%
!!! CAGTAATTTTAAGTGTTGAT+GGG + chr8_3:47489886-47489905 None:intergenic 25.0%
!! ATTGATTGATCTTTTCCTTT+TGG - chr8_3:47490131-47490150 Msa1291910:three_prime_UTR 25.0%
!!! ATATATTCCTTTCTAGAATC+CGG - chr8_3:47490565-47490584 Msa1291910:CDS 25.0%
! CTATGTCTTTCTTCAATAAT+AGG - chr8_3:47491127-47491146 Msa1291910:intron 25.0%
! ATGGTGGCTATATATAATAT+GGG + chr8_3:47491190-47491209 None:intergenic 25.0%
! AATGGTGGCTATATATAATA+TGG + chr8_3:47491191-47491210 None:intergenic 25.0%
!! GATTTTGTAGTGTACAATAT+TGG + chr8_3:47491866-47491885 None:intergenic 25.0%
!!! CTTGATTTTCTGCTTATTTT+TGG - chr8_3:47491927-47491946 Msa1291910:intron 25.0%
! AATAAGAAGTTAAGAACCAA+TGG - chr8_3:47491975-47491994 Msa1291910:intron 25.0%
!!! AATGATTTTTTGAATCGTTG+AGG + chr8_3:47492103-47492122 None:intergenic 25.0%
!! GATGTAGATTTTTATAAGGA+AGG - chr8_3:47492317-47492336 Msa1291910:intron 25.0%
!! CAAAATATTGATAGCATACT+CGG + chr8_3:47492401-47492420 None:intergenic 25.0%
! AAAAACGATAGGAATCAAAA+TGG - chr8_3:47492493-47492512 Msa1291910:intron 25.0%
! AAATGATCATCATCTACAAA+GGG + chr8_3:47492691-47492710 None:intergenic 25.0%
! ATTAATATGGCATATTCTTG+GGG + chr8_3:47492733-47492752 None:intergenic 25.0%
! GTATTAATATGGCATATTCT+TGG + chr8_3:47492735-47492754 None:intergenic 25.0%
! TGCCTCTTATGTATATTTAT+TGG - chr8_3:47492781-47492800 Msa1291910:intron 25.0%
! GCCTCTTATGTATATTTATT+GGG - chr8_3:47492782-47492801 Msa1291910:intron 25.0%
! ACCCAATAAATATACATAAG+AGG + chr8_3:47492786-47492805 None:intergenic 25.0%
! TGAGAAAATTAAAGGGAAAA+CGG + chr8_3:47493057-47493076 None:intergenic 25.0%
! ACAGGAATGAGAAAATTAAA+GGG + chr8_3:47493064-47493083 None:intergenic 25.0%
! AACAGGAATGAGAAAATTAA+AGG + chr8_3:47493065-47493084 None:intergenic 25.0%
! GATCTAAATCTCACATATAA+CGG - chr8_3:47493201-47493220 Msa1291910:five_prime_UTR 25.0%
!! CACTTTAAAAAGTGGTAATA+AGG + chr8_3:47493255-47493274 None:intergenic 25.0%
!!! AATTTTAAGTGTTGATGGGT+TGG + chr8_3:47489882-47489901 None:intergenic 30.0%
!!! GCAGTAATTTTAAGTGTTGA+TGG + chr8_3:47489887-47489906 None:intergenic 30.0%
TCTGAATCCTCATACATTAA+GGG + chr8_3:47489914-47489933 None:intergenic 30.0%
TTCTGAATCCTCATACATTA+AGG + chr8_3:47489915-47489934 None:intergenic 30.0%
TATGAGGATTCAGAAAACAT+TGG - chr8_3:47489920-47489939 Msa1291910:three_prime_UTR 30.0%
GTGATTCGATCTAAAAAAGA+GGG + chr8_3:47490416-47490435 None:intergenic 30.0%
!!! TCTTTTTTAGATCGAATCAC+CGG - chr8_3:47490416-47490435 Msa1291910:CDS 30.0%
GTACAAAATAGTAGCATTTC+CGG + chr8_3:47490438-47490457 None:intergenic 30.0%
TACAATTAAGGACGTTATTC+AGG + chr8_3:47490508-47490527 None:intergenic 30.0%
! GGATTCTAGAAAGGAATATA+TGG + chr8_3:47490566-47490585 None:intergenic 30.0%
ACAGGAAAATTCAACATAGA+CGG + chr8_3:47490610-47490629 None:intergenic 30.0%
! CTTCTTTTAACCGAAGAATA+TGG - chr8_3:47490676-47490695 Msa1291910:intron 30.0%
CAACTAAAAGAACTAGATGA+AGG - chr8_3:47490708-47490727 Msa1291910:intron 30.0%
!! TGAGAAAATTTTGATGGAGA+GGG - chr8_3:47490764-47490783 Msa1291910:intron 30.0%
! AGGATGATTTTATAAAGGGT+TGG - chr8_3:47490855-47490874 Msa1291910:intron 30.0%
TTGCATGCTAAGATGATTAA+GGG + chr8_3:47490996-47491015 None:intergenic 30.0%
ATTAGGAAAAGGATGGTTAT+TGG - chr8_3:47491017-47491036 Msa1291910:intron 30.0%
TTAGGAAAAGGATGGTTATT+GGG - chr8_3:47491018-47491037 Msa1291910:intron 30.0%
TGGTGGCTATATATAATATG+GGG + chr8_3:47491189-47491208 None:intergenic 30.0%
ACTTCAATATAAACTGAAGC+AGG + chr8_3:47491233-47491252 None:intergenic 30.0%
TTGACATGAATGAACCAAAA+TGG + chr8_3:47491358-47491377 None:intergenic 30.0%
AATATCTGTAGCAAATGAGA+TGG - chr8_3:47491405-47491424 Msa1291910:intron 30.0%
ATGAAGTCAAATTGAGTAGA+AGG + chr8_3:47491562-47491581 None:intergenic 30.0%
TCCTCTTCACAAAAATACAA+AGG - chr8_3:47491789-47491808 Msa1291910:intron 30.0%
!! ACCTTTGTATTTTTGTGAAG+AGG + chr8_3:47491793-47491812 None:intergenic 30.0%
ATGAATCAATTGTCTAGGTT+CGG + chr8_3:47491889-47491908 None:intergenic 30.0%
!!! TATTTTTGGAGCCTATTAAC+AGG - chr8_3:47491941-47491960 Msa1291910:intron 30.0%
!!! ATTTTTGGAGCCTATTAACA+GGG - chr8_3:47491942-47491961 Msa1291910:intron 30.0%
TAACCAATTTCCCTGTTAAT+AGG + chr8_3:47491955-47491974 None:intergenic 30.0%
ACCAGTTTGACAAATCAATT+TGG - chr8_3:47492035-47492054 Msa1291910:intron 30.0%
!! TCCAAATTGATTTGTCAAAC+TGG + chr8_3:47492039-47492058 None:intergenic 30.0%
TTTAAATTCCCAAACAGAAC+TGG + chr8_3:47492076-47492095 None:intergenic 30.0%
GAATATCGATAAGCTTATAC+AGG + chr8_3:47492552-47492571 None:intergenic 30.0%
GAAATGATCATCATCTACAA+AGG + chr8_3:47492692-47492711 None:intergenic 30.0%
AGCAGAAACAGGTATTAATA+TGG + chr8_3:47492746-47492765 None:intergenic 30.0%
!! ATATTAATACCTGTTTCTGC+TGG - chr8_3:47492745-47492764 Msa1291910:intron 30.0%
CTTTATACGATCGATTGAGA+AGG + chr8_3:47489977-47489996 None:intergenic 35.0%
CGTATAAAGAAAAAGTTCGC+CGG - chr8_3:47489988-47490007 Msa1291910:three_prime_UTR 35.0%
ACAATGTTTCTTACCGTTCA+CGG + chr8_3:47490042-47490061 None:intergenic 35.0%
!!! TTCCTTTTGGTTAAGTTGAG+TGG - chr8_3:47490144-47490163 Msa1291910:three_prime_UTR 35.0%
ATCCACTCAACTTAACCAAA+AGG + chr8_3:47490149-47490168 None:intergenic 35.0%
AGTCAAGAATGATTCGAATG+AGG - chr8_3:47490344-47490363 Msa1291910:CDS 35.0%
GTCAAGAATGATTCGAATGA+GGG - chr8_3:47490345-47490364 Msa1291910:CDS 35.0%
GGTGATTCGATCTAAAAAAG+AGG + chr8_3:47490417-47490436 None:intergenic 35.0%
CGTTATTCAGGACAAAGAAA+AGG + chr8_3:47490496-47490515 None:intergenic 35.0%
AGGAAAATTCAACATAGACG+GGG + chr8_3:47490608-47490627 None:intergenic 35.0%
CAGGAAAATTCAACATAGAC+GGG + chr8_3:47490609-47490628 None:intergenic 35.0%
GAAATTGGGAGTGAATACTT+GGG - chr8_3:47490640-47490659 Msa1291910:exon 35.0%
AAATTGGGAGTGAATACTTG+GGG - chr8_3:47490641-47490660 Msa1291910:five_prime_UTR 35.0%
! GCTTGCTAAACCATATTCTT+CGG + chr8_3:47490689-47490708 None:intergenic 35.0%
!! ACGGACTGAGAAAATTTTGA+TGG - chr8_3:47490758-47490777 Msa1291910:intron 35.0%
!! CTGAGAAAATTTTGATGGAG+AGG - chr8_3:47490763-47490782 Msa1291910:intron 35.0%
TGCATGCTAAGATGATTAAG+GGG + chr8_3:47490995-47491014 None:intergenic 35.0%
GTTGCATGCTAAGATGATTA+AGG + chr8_3:47490997-47491016 None:intergenic 35.0%
TCATCTTAGCATGCAACATT+AGG - chr8_3:47491000-47491019 Msa1291910:intron 35.0%
TAGCATGCAACATTAGGAAA+AGG - chr8_3:47491006-47491025 Msa1291910:intron 35.0%
ATGCAACATTAGGAAAAGGA+TGG - chr8_3:47491010-47491029 Msa1291910:intron 35.0%
! ACCATTCACACTAGTTTGAT+GGG - chr8_3:47491205-47491224 Msa1291910:intron 35.0%
CTAATGTCTAGGTGAAAGAT+TGG + chr8_3:47491274-47491293 None:intergenic 35.0%
GCTAAGACAATGACAAATTG+GGG + chr8_3:47491433-47491452 None:intergenic 35.0%
GGCTAAGACAATGACAAATT+GGG + chr8_3:47491434-47491453 None:intergenic 35.0%
CAATTTGTCATTGTCTTAGC+CGG - chr8_3:47491433-47491452 Msa1291910:intron 35.0%
ATCACATTATCACTAGCTAC+TGG + chr8_3:47491495-47491514 None:intergenic 35.0%
AATTCCTATCAAGTTCACTC+TGG + chr8_3:47491524-47491543 None:intergenic 35.0%
! ATTTGACTTCATCCAATGCT+TGG - chr8_3:47491570-47491589 Msa1291910:intron 35.0%
GAAGAGGAACTATTACTATG+AGG + chr8_3:47491777-47491796 None:intergenic 35.0%
TGAATCAATTGTCTAGGTTC+GGG + chr8_3:47491888-47491907 None:intergenic 35.0%
AGGGTATGAATCAATTGTCT+AGG + chr8_3:47491894-47491913 None:intergenic 35.0%
! GATTCATACCCTCATTTTCA+TGG - chr8_3:47491902-47491921 Msa1291910:intron 35.0%
ATCAAGCACCATGAAAATGA+GGG + chr8_3:47491913-47491932 None:intergenic 35.0%
AATCAAGCACCATGAAAATG+AGG + chr8_3:47491914-47491933 None:intergenic 35.0%
TCAAAAACTGCTTGATCCAT+TGG + chr8_3:47491994-47492013 None:intergenic 35.0%
! AAACTGGTTTCTGAACATGT+TGG + chr8_3:47492023-47492042 None:intergenic 35.0%
TTCAGAAACCAGTTCTGTTT+GGG - chr8_3:47492065-47492084 Msa1291910:intron 35.0%
CATCAAATTGATGAATGCGA+AGG + chr8_3:47492301-47492320 None:intergenic 35.0%
!! TGTGTATTTTTGTTGTCGAC+AGG - chr8_3:47492466-47492485 Msa1291910:intron 35.0%
ACATTATGCCTTCTTCTTCT+TGG + chr8_3:47492824-47492843 None:intergenic 35.0%
GTGCTGTAGATACTACATTA+AGG + chr8_3:47493012-47493031 None:intergenic 35.0%
AAATTAAAGGGAAAACGGAG+AGG + chr8_3:47493052-47493071 None:intergenic 35.0%
! GTCTCTATCTCTTTTCTAAC+AGG + chr8_3:47493082-47493101 None:intergenic 35.0%
TGCTTTGCCCTTAATGTATG+AGG - chr8_3:47489904-47489923 Msa1291910:three_prime_UTR 40.0%
ATACGATCGATTGAGAAGGT+AGG + chr8_3:47489973-47489992 None:intergenic 40.0%
! TACTTGTCCGGATTCTAGAA+AGG + chr8_3:47490575-47490594 None:intergenic 40.0%
GGTAAGTTCAGTTACTTGTC+CGG + chr8_3:47490587-47490606 None:intergenic 40.0%
CGAAATTGGGAGTGAATACT+TGG - chr8_3:47490639-47490658 Msa1291910:exon 40.0%
AATACTTGGGGTTGAAGCTA+CGG - chr8_3:47490653-47490672 Msa1291910:intron 40.0%
ACTAGATGAAGGCAAAGAGA+TGG - chr8_3:47490719-47490738 Msa1291910:intron 40.0%
CTAGATGAAGGCAAAGAGAT+GGG - chr8_3:47490720-47490739 Msa1291910:intron 40.0%
CATCAAACTAGTGTGAATGG+TGG + chr8_3:47491206-47491225 None:intergenic 40.0%
! CACCATTCACACTAGTTTGA+TGG - chr8_3:47491204-47491223 Msa1291910:intron 40.0%
! AACTCTTGCCTCGCTTTAAA+TGG - chr8_3:47491315-47491334 Msa1291910:intron 40.0%
! ACTCTTGCCTCGCTTTAAAT+GGG - chr8_3:47491316-47491335 Msa1291910:intron 40.0%
!! TTTTCGACCCATTTAAAGCG+AGG + chr8_3:47491326-47491345 None:intergenic 40.0%
CGGCTAAGACAATGACAAAT+TGG + chr8_3:47491435-47491454 None:intergenic 40.0%
TCAACATTGACGACACAAGT+TGG + chr8_3:47491462-47491481 None:intergenic 40.0%
TGTCGTCAATGTTGATCAGA+CGG - chr8_3:47491467-47491486 Msa1291910:intron 40.0%
AAGTAAGCAAGTCCAAGCAT+TGG + chr8_3:47491585-47491604 None:intergenic 40.0%
! TGCTTGGACTTGCTTACTTT+AGG - chr8_3:47491586-47491605 Msa1291910:intron 40.0%
TCAGTATTCATACAACCCAC+AGG - chr8_3:47491684-47491703 Msa1291910:intron 40.0%
CACAAGACAAAGAATCCTGT+GGG + chr8_3:47491702-47491721 None:intergenic 40.0%
GATTCTTTGTCTTGTGCAGA+TGG - chr8_3:47491706-47491725 Msa1291910:intron 40.0%
GAGCCTATTAACAGGGAAAT+TGG - chr8_3:47491949-47491968 Msa1291910:intron 40.0%
GTTCAGAAACCAGTTCTGTT+TGG - chr8_3:47492064-47492083 Msa1291910:intron 40.0%
AATCATTAGAGTTCGGTCCA+TGG - chr8_3:47492116-47492135 Msa1291910:intron 40.0%
!! TAACCACTTTTGGTACTCCA+TGG + chr8_3:47492136-47492155 None:intergenic 40.0%
GTACCAAAAGTGGTTAAGCT+TGG - chr8_3:47492140-47492159 Msa1291910:intron 40.0%
! GAACCAAGCTTAACCACTTT+TGG + chr8_3:47492146-47492165 None:intergenic 40.0%
GAACCATGAATACTCTGTCA+TGG - chr8_3:47492214-47492233 Msa1291910:intron 40.0%
! AGACCATGACAGAGTATTCA+TGG + chr8_3:47492220-47492239 None:intergenic 40.0%
TGCAATGAGCTGAAAGGAAT+TGG + chr8_3:47492357-47492376 None:intergenic 40.0%
AAAGTCTGCAATGAGCTGAA+AGG + chr8_3:47492363-47492382 None:intergenic 40.0%
TACTTTCTGGGTTATCATCC+TGG - chr8_3:47492580-47492599 Msa1291910:intron 40.0%
TACTTGGCATGTTACTAACC+AGG + chr8_3:47492601-47492620 None:intergenic 40.0%
GCTCAAATCCAAGAAGAAGA+AGG - chr8_3:47492813-47492832 Msa1291910:intron 40.0%
GCTACAAAAACGAGGTAATG+TGG + chr8_3:47492970-47492989 None:intergenic 40.0%
!! CATTACCTCGTTTTTGTAGC+TGG - chr8_3:47492970-47492989 Msa1291910:intron 40.0%
AGTATCTACAGCACACAACA+TGG - chr8_3:47493018-47493037 Msa1291910:intron 40.0%
TACAGCACACAACATGGATT+TGG - chr8_3:47493024-47493043 Msa1291910:intron 40.0%
! ATAAACACCGGTGGCTTTTC+CGG + chr8_3:47490010-47490029 None:intergenic 45.0%
ATGTTTCTTACCGTTCACGG+CGG + chr8_3:47490039-47490058 None:intergenic 45.0%
! AATGAGGGAGAACTTTTGCC+CGG - chr8_3:47490360-47490379 Msa1291910:CDS 45.0%
TGAAACCTTCCTCCTCAAAC+CGG + chr8_3:47490382-47490401 None:intergenic 45.0%
GGACAAAGAAAAGGATGCAC+AGG + chr8_3:47490487-47490506 None:intergenic 45.0%
CTGTGTCTTGCGTCGAAATT+GGG - chr8_3:47490626-47490645 Msa1291910:exon 45.0%
GGGTGAGTGTGGAAGATAAT+CGG - chr8_3:47490784-47490803 Msa1291910:intron 45.0%
GCCCATCAAACTAGTGTGAA+TGG + chr8_3:47491209-47491228 None:intergenic 45.0%
!! TGACGACACAAGTTGGTTAC+CGG + chr8_3:47491455-47491474 None:intergenic 45.0%
ACCACCAGAGTGAACTTGAT+AGG - chr8_3:47491517-47491536 Msa1291910:intron 45.0%
TCCTATCAAGTTCACTCTGG+TGG + chr8_3:47491521-47491540 None:intergenic 45.0%
GCACAAGACAAAGAATCCTG+TGG + chr8_3:47491703-47491722 None:intergenic 45.0%
! TAAGCTTGGTTCGACTGAAC+TGG - chr8_3:47492154-47492173 Msa1291910:intron 45.0%
CGACAGGAAGCAAAAACGAT+AGG - chr8_3:47492482-47492501 Msa1291910:intron 45.0%
CTATGTATGACCCTAGTGTC+AGG - chr8_3:47492935-47492954 Msa1291910:intron 45.0%
ATGACCCTAGTGTCAGGAAT+TGG - chr8_3:47492941-47492960 Msa1291910:intron 45.0%
GTAGCCAATTCCTGACACTA+GGG + chr8_3:47492948-47492967 None:intergenic 45.0%
!! TACCTCGTTTTTGTAGCTGG+CGG - chr8_3:47492973-47492992 Msa1291910:intron 45.0%
TTCACCCTCATGTAAGGAGA+CGG + chr8_3:47493173-47493192 None:intergenic 45.0%
CTCCTTACATGAGGGTGAAA+AGG - chr8_3:47493174-47493193 Msa1291910:five_prime_UTR 45.0%
! CTCCTTTTCACCCTCATGTA+AGG + chr8_3:47493179-47493198 None:intergenic 45.0%
TACATGAGGGTGAAAAGGAG+AGG - chr8_3:47493179-47493198 Msa1291910:five_prime_UTR 45.0%
!!! TTTTATTTTGTATTTTTATT+TGG + chr8_3:47490817-47490836 None:intergenic 5.0%
AAGCCACCGGTGTTTATCGT+CGG - chr8_3:47490013-47490032 Msa1291910:three_prime_UTR 50.0%
!! GAGAACTTTTGCCCGGTTTG+AGG - chr8_3:47490367-47490386 Msa1291910:CDS 50.0%
!! AACTTTTGCCCGGTTTGAGG+AGG - chr8_3:47490370-47490389 Msa1291910:CDS 50.0%
GAAACCTTCCTCCTCAAACC+GGG + chr8_3:47490381-47490400 None:intergenic 50.0%
CCAATTTCGACGCAAGACAC+AGG + chr8_3:47490628-47490647 None:intergenic 50.0%
CCTGTGTCTTGCGTCGAAAT+TGG - chr8_3:47490625-47490644 Msa1291910:exon 50.0%
! TGGGAGTGTGTGGAAGATGA+CGG - chr8_3:47490739-47490758 Msa1291910:intron 50.0%
TTTGATGGAGAGGGTGAGTG+TGG - chr8_3:47490773-47490792 Msa1291910:intron 50.0%
! GGTCCATGGAGTACCAAAAG+TGG - chr8_3:47492130-47492149 Msa1291910:intron 50.0%
GAACTAGCAGACAGAGCAGT+TGG - chr8_3:47492515-47492534 Msa1291910:intron 50.0%
AGAGCAACACCAGCAGAAAC+AGG + chr8_3:47492757-47492776 None:intergenic 50.0%
GGTAGCCAATTCCTGACACT+AGG + chr8_3:47492949-47492968 None:intergenic 50.0%
!! ACCTCGTTTTTGTAGCTGGC+GGG - chr8_3:47492974-47492993 Msa1291910:intron 50.0%
TGTGTCCGTCTCCTTACATG+AGG - chr8_3:47493165-47493184 Msa1291910:five_prime_UTR 50.0%
GTGTCCGTCTCCTTACATGA+GGG - chr8_3:47493166-47493185 Msa1291910:five_prime_UTR 50.0%
AAGTTCGCCGGAAAAGCCAC+CGG - chr8_3:47490000-47490019 Msa1291910:three_prime_UTR 55.0%
! TTTGCCCGGTTTGAGGAGGA+AGG - chr8_3:47490374-47490393 Msa1291910:CDS 55.0%
! GGCAAAGAGATGGGAGTGTG+TGG - chr8_3:47490729-47490748 Msa1291910:intron 55.0%
!! CCTCGTTTTTGTAGCTGGCG+GGG - chr8_3:47492975-47492994 Msa1291910:intron 55.0%
TTATCGTCGGCCGCCGTGAA+CGG - chr8_3:47490026-47490045 Msa1291910:three_prime_UTR 60.0%
CCCCGCCAGCTACAAAAACG+AGG + chr8_3:47492978-47492997 None:intergenic 60.0%
CGGCCGACGATAAACACCGG+TGG + chr8_3:47490019-47490038 None:intergenic 65.0%
CGGCGGCCGACGATAAACAC+CGG + chr8_3:47490022-47490041 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 47489856 47493296 47489856 ID=Msa1291910;Name=Msa1291910
chr8_3 mRNA 47489856 47493296 47489856 ID=Msa1291910-mRNA-1;Parent=Msa1291910;Name=Msa1291910-mRNA-1;_AED=0.01;_eAED=0.01;_QI=216|1|1|1|0.5|1|3|456|80
chr8_3 exon 47489856 47490464 47489856 ID=Msa1291910-mRNA-1:exon:13257;Parent=Msa1291910-mRNA-1
chr8_3 exon 47490551 47490664 47490551 ID=Msa1291910-mRNA-1:exon:13256;Parent=Msa1291910-mRNA-1
chr8_3 exon 47493105 47493296 47493105 ID=Msa1291910-mRNA-1:exon:13255;Parent=Msa1291910-mRNA-1
chr8_3 five_prime_UTR 47493105 47493296 47493105 ID=Msa1291910-mRNA-1:five_prime_utr;Parent=Msa1291910-mRNA-1
chr8_3 five_prime_UTR 47490641 47490664 47490641 ID=Msa1291910-mRNA-1:five_prime_utr;Parent=Msa1291910-mRNA-1
chr8_3 CDS 47490551 47490640 47490551 ID=Msa1291910-mRNA-1:cds;Parent=Msa1291910-mRNA-1
chr8_3 CDS 47490312 47490464 47490312 ID=Msa1291910-mRNA-1:cds;Parent=Msa1291910-mRNA-1
chr8_3 three_prime_UTR 47489856 47490311 47489856 ID=Msa1291910-mRNA-1:three_prime_utr;Parent=Msa1291910-mRNA-1
Gene Sequence

>Msa1291910

ATGGAACTAGCAGACAGAGCAGTTGGATTTCTATTGTCCTGTATAAGCTTATCGATATTCACTTATTATACTTTCTGGGTTATCATCCTGCCCTTTGTAGATGATGATCATTTCGTACACAAGTATTTCTTACCCCAAGAATATGCCATATTAATACCTGTTTCTGCTGGTGTTGCTCTTATTTGCCTCTTATGTATATTTATTGGGTTCGTGATGCTCAAATCCAAGAAGAAGAAGGCATAA
Protein sequence

>Msa1291910

MELADRAVGFLLSCISLSIFTYYTFWVIILPFVDDDHFVHKYFLPQEYAILIPVSAGVALICLLCIFIGFVMLKSKKKKA*