AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1253190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1253190 MtrunA17_Chr8g0369771 93.782 193 12 0 1 193 1 193 5.08e-134 373
Msa1253190 MtrunA17_Chr5g0408221 67.232 177 57 1 5 181 4 179 4.57e-78 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1253190 AT1G31720.2 39.474 152 88 1 27 178 29 176 5.57e-37 127
Msa1253190 AT4G19370.1 35.176 199 96 4 10 182 7 198 2.15e-36 126
Msa1253190 AT1G31720.6 40.411 146 83 1 27 172 29 170 4.47e-36 124
Msa1253190 AT1G31720.4 38.462 156 88 2 27 178 29 180 1.10e-34 121
Msa1253190 AT1G31720.1 37.037 162 88 2 27 178 29 186 5.15e-34 119
Msa1253190 AT1G31720.3 39.333 150 83 2 27 172 29 174 9.83e-34 119
Msa1253190 AT1G31720.7 37.821 156 83 2 27 172 29 180 5.40e-33 117
Msa1253190 AT1G31720.5 39.437 142 82 1 40 181 39 176 7.98e-31 111

Find 54 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACAATTGGTTCTCTAAA+TGG 0.171028 8_2:+50509695 Msa1253190:CDS
GGTGAGTGCTTTCTAGTTAA+AGG 0.216884 8_2:+50509638 Msa1253190:CDS
AGATCATAAAATACACAAAT+TGG 0.276980 8_2:+50509757 Msa1253190:CDS
TTTCCTTCTTTCTTCCTTTG+TGG 0.301602 8_2:-50508799 None:intergenic
AATCCAAATGCTTGGCTTGT+TGG 0.330057 8_2:-50509288 None:intergenic
TCCTATTGAGTCTACCATAT+TGG 0.343800 8_2:-50508761 None:intergenic
TTGCCAACAAGCCAAGCATT+TGG 0.378927 8_2:+50509285 Msa1253190:CDS
AATTGTAACAAGGATCAATA+TGG 0.381978 8_2:-50509681 None:intergenic
ATTATAATTTCTCTCTCCCT+TGG 0.386629 8_2:+50508996 Msa1253190:CDS
ATGATCTTGATGTGCTTGGC+TGG 0.390022 8_2:-50509741 None:intergenic
TATAGGAGGAGGATCTTAGA+TGG 0.392354 8_2:+50509244 Msa1253190:intron
TGCTGAGATAAAGAGGAATA+AGG 0.392430 8_2:+50509046 Msa1253190:CDS
ATGAGCAGAAGACAGCTCTA+TGG 0.398914 8_2:+50509599 Msa1253190:CDS
AAGAAAAGGGGTTTGGAAAG+TGG 0.400919 8_2:+50509820 Msa1253190:three_prime_UTR
ATATTGATCCTTGTTACAAT+TGG 0.401090 8_2:+50509683 Msa1253190:CDS
GCATCTTCTTCAAACCAATA+TGG 0.410593 8_2:+50508747 Msa1253190:five_prime_UTR
ACCAATATGGTAGACTCAAT+AGG 0.453450 8_2:+50508760 Msa1253190:five_prime_UTR
ATGTTTATCAGGCTTAGCTT+TGG 0.454000 8_2:+50509542 Msa1253190:intron
AGAAAAGGGGTTTGGAAAGT+GGG 0.466215 8_2:+50509821 Msa1253190:three_prime_UTR
CAAAGGAAGAAAGAAGGAAA+GGG 0.473670 8_2:+50508802 Msa1253190:five_prime_UTR
TGGATTGAGTATTGCAACAT+TGG 0.477989 8_2:+50509305 Msa1253190:CDS
CAATACTCAATCCAAATGCT+TGG 0.501870 8_2:-50509296 None:intergenic
TTACAAACTCGTGCTTCGCA+CGG 0.506570 8_2:+50509961 Msa1253190:three_prime_UTR
AGACTCAATAGGAAGAAAGT+TGG 0.508562 8_2:+50508771 Msa1253190:five_prime_UTR
TACAAACTCGTGCTTCGCAC+GGG 0.509699 8_2:+50509962 Msa1253190:three_prime_UTR
AGTAGTAGGCATCTTAAACT+TGG 0.515837 8_2:-50509403 None:intergenic
GAGGAGGATCTTAGATGGAA+TGG 0.517161 8_2:+50509249 Msa1253190:CDS
TGAGCAGAAGACAGCTCTAT+GGG 0.525248 8_2:+50509600 Msa1253190:CDS
GCCTCTACAACAACTTGTGG+AGG 0.529266 8_2:-50509877 None:intergenic
GTATAGCTGCTGAGATAAAG+AGG 0.531546 8_2:+50509039 Msa1253190:CDS
AACAGAACCTTTGCAGTAGT+AGG 0.536472 8_2:-50509417 None:intergenic
ACACCACAAAGGAAGAAAGA+AGG 0.537557 8_2:+50508796 Msa1253190:five_prime_UTR
TATGGGAAAGGATGGTTGAA+TGG 0.549765 8_2:+50509617 Msa1253190:CDS
ATCACCGTGTACTTGCATCT+AGG 0.552747 8_2:-50508963 None:intergenic
GACAGCTCTATGGGAAAGGA+TGG 0.557600 8_2:+50509609 Msa1253190:CDS
AGCAGCTATACACAAGATGA+AGG 0.559646 8_2:-50509027 None:intergenic
ACAAAGGAAGAAAGAAGGAA+AGG 0.562436 8_2:+50508801 Msa1253190:five_prime_UTR
GTTAAAGGAGGAACTTACTC+TGG 0.566362 8_2:+50509653 Msa1253190:CDS
TTAGCCTCTACAACAACTTG+TGG 0.567520 8_2:-50509880 None:intergenic
TAAGATGCCTACTACTGCAA+AGG 0.579628 8_2:+50509410 Msa1253190:CDS
CCCTCCACAAGTTGTTGTAG+AGG 0.594872 8_2:+50509876 Msa1253190:three_prime_UTR
CTCTACAACAACTTGTGGAG+GGG 0.597248 8_2:-50509875 None:intergenic
CCTCTACAACAACTTGTGGA+GGG 0.602704 8_2:-50509876 None:intergenic
AGAAGACAGCTCTATGGGAA+AGG 0.604427 8_2:+50509605 Msa1253190:CDS
TATGTCTTGAAACTTACTCA+AGG 0.607437 8_2:-50508864 None:intergenic
GATGAAGGAGATTAAGCCAA+GGG 0.614196 8_2:-50509012 None:intergenic
TCCTCCTAGATGCAAGTACA+CGG 0.621959 8_2:+50508959 Msa1253190:CDS
ACTTGATGCTATGAAGCTGT+TGG 0.629641 8_2:-50508829 None:intergenic
GAGTGCTTTCTAGTTAAAGG+AGG 0.633792 8_2:+50509641 Msa1253190:CDS
ACCGTGTACTTGCATCTAGG+AGG 0.634973 8_2:-50508960 None:intergenic
GGAAGAAAGAAGGAAAGGGA+TGG 0.636143 8_2:+50508806 Msa1253190:five_prime_UTR
AGATGAAGGAGATTAAGCCA+AGG 0.643907 8_2:-50509013 None:intergenic
TTAGAGAACCAATTGTAACA+AGG 0.669588 8_2:-50509691 None:intergenic
GAAAGTTGGAAACACCACAA+AGG 0.697818 8_2:+50508785 Msa1253190:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAATTCTTATTCAAGA+TGG + chr8_2:50509370-50509389 Msa1253190:CDS 15.0%
!! TAAAAAATTCTTATTCAAGA+TGG + chr8_2:50509370-50509389 Msa1253190:CDS 15.0%
!! AAAATAAACAAATCAAGCAT+TGG - chr8_2:50509225-50509244 None:intergenic 20.0%
!!! GCTTGATTTGTTTATTTTAT+AGG + chr8_2:50509227-50509246 Msa1253190:intron 20.0%
!!! TGATTTGTTTATTTTATAGG+AGG + chr8_2:50509230-50509249 Msa1253190:intron 20.0%
!!! TTATCTTTTGCACATATAAT+TGG + chr8_2:50509333-50509352 Msa1253190:CDS 20.0%
!! AATTGTTTGTAATGTTTATC+AGG + chr8_2:50509531-50509550 Msa1253190:intron 20.0%
!! AGATCATAAAATACACAAAT+TGG + chr8_2:50509757-50509776 Msa1253190:CDS 20.0%
!! TGTTAGAATAGAAAAAGAAA+AGG + chr8_2:50509806-50509825 Msa1253190:exon 20.0%
!! GTTAGAATAGAAAAAGAAAA+GGG + chr8_2:50509807-50509826 Msa1253190:exon 20.0%
!! TTAGAATAGAAAAAGAAAAG+GGG + chr8_2:50509808-50509827 Msa1253190:exon 20.0%
!! TTGTAAAAAAATTGTTCCAA+AGG + chr8_2:50509999-50510018 Msa1253190:three_prime_UTR 20.0%
!!! TAATTTACTCATTTTTCCTT+TGG - chr8_2:50510018-50510037 None:intergenic 20.0%
!! AAAATAAACAAATCAAGCAT+TGG - chr8_2:50509225-50509244 None:intergenic 20.0%
!!! GCTTGATTTGTTTATTTTAT+AGG + chr8_2:50509227-50509246 Msa1253190:intron 20.0%
!!! TGATTTGTTTATTTTATAGG+AGG + chr8_2:50509230-50509249 Msa1253190:intron 20.0%
!!! TTATCTTTTGCACATATAAT+TGG + chr8_2:50509333-50509352 Msa1253190:CDS 20.0%
!! AATTGTTTGTAATGTTTATC+AGG + chr8_2:50509531-50509550 Msa1253190:intron 20.0%
!! AGATCATAAAATACACAAAT+TGG + chr8_2:50509757-50509776 Msa1253190:CDS 20.0%
!! TGTTAGAATAGAAAAAGAAA+AGG + chr8_2:50509806-50509825 Msa1253190:exon 20.0%
!! GTTAGAATAGAAAAAGAAAA+GGG + chr8_2:50509807-50509826 Msa1253190:exon 20.0%
!! TTAGAATAGAAAAAGAAAAG+GGG + chr8_2:50509808-50509827 Msa1253190:exon 20.0%
!! TTGTAAAAAAATTGTTCCAA+AGG + chr8_2:50509999-50510018 Msa1253190:three_prime_UTR 20.0%
!!! TAATTTACTCATTTTTCCTT+TGG - chr8_2:50510018-50510037 None:intergenic 20.0%
! CAAAAACAAAAAATCCATCA+TGG + chr8_2:50508929-50508948 Msa1253190:exon 25.0%
! TGAGAAAATCAATCAATTTG+AGG - chr8_2:50509118-50509137 None:intergenic 25.0%
!!! TTTGTTTATTTTATAGGAGG+AGG + chr8_2:50509233-50509252 Msa1253190:intron 25.0%
! AATTGTAACAAGGATCAATA+TGG - chr8_2:50509684-50509703 None:intergenic 25.0%
! ATATTGATCCTTGTTACAAT+TGG + chr8_2:50509683-50509702 Msa1253190:CDS 25.0%
! CAAAAACAAAAAATCCATCA+TGG + chr8_2:50508929-50508948 Msa1253190:exon 25.0%
! TGAGAAAATCAATCAATTTG+AGG - chr8_2:50509118-50509137 None:intergenic 25.0%
!!! TTTGTTTATTTTATAGGAGG+AGG + chr8_2:50509233-50509252 Msa1253190:intron 25.0%
! AATTGTAACAAGGATCAATA+TGG - chr8_2:50509684-50509703 None:intergenic 25.0%
! ATATTGATCCTTGTTACAAT+TGG + chr8_2:50509683-50509702 Msa1253190:CDS 25.0%
TATGTCTTGAAACTTACTCA+AGG - chr8_2:50508867-50508886 None:intergenic 30.0%
AGACTTGTGAAAAAAGTAGA+AGG - chr8_2:50508902-50508921 None:intergenic 30.0%
ATTATAATTTCTCTCTCCCT+TGG + chr8_2:50508996-50509015 Msa1253190:CDS 30.0%
AGATAAAGAGGAATAAGGTT+AGG + chr8_2:50509051-50509070 Msa1253190:intron 30.0%
!!! AGGTTCTGTTTTTGATATCT+TGG + chr8_2:50509430-50509449 Msa1253190:CDS 30.0%
TTAGAGAACCAATTGTAACA+AGG - chr8_2:50509694-50509713 None:intergenic 30.0%
! GTTACAATTGGTTCTCTAAA+TGG + chr8_2:50509695-50509714 Msa1253190:CDS 30.0%
! TTTTATGATCTTGATGTGCT+TGG - chr8_2:50509748-50509767 None:intergenic 30.0%
CTATTCTAACACAAAAAGTG+TGG - chr8_2:50509797-50509816 None:intergenic 30.0%
ATAGAAAAAGAAAAGGGGTT+TGG + chr8_2:50509813-50509832 Msa1253190:three_prime_UTR 30.0%
TATGTCTTGAAACTTACTCA+AGG - chr8_2:50508867-50508886 None:intergenic 30.0%
AGACTTGTGAAAAAAGTAGA+AGG - chr8_2:50508902-50508921 None:intergenic 30.0%
ATTATAATTTCTCTCTCCCT+TGG + chr8_2:50508996-50509015 Msa1253190:CDS 30.0%
AGATAAAGAGGAATAAGGTT+AGG + chr8_2:50509051-50509070 Msa1253190:intron 30.0%
!!! AGGTTCTGTTTTTGATATCT+TGG + chr8_2:50509430-50509449 Msa1253190:CDS 30.0%
TTAGAGAACCAATTGTAACA+AGG - chr8_2:50509694-50509713 None:intergenic 30.0%
! GTTACAATTGGTTCTCTAAA+TGG + chr8_2:50509695-50509714 Msa1253190:CDS 30.0%
! TTTTATGATCTTGATGTGCT+TGG - chr8_2:50509748-50509767 None:intergenic 30.0%
CTATTCTAACACAAAAAGTG+TGG - chr8_2:50509797-50509816 None:intergenic 30.0%
ATAGAAAAAGAAAAGGGGTT+TGG + chr8_2:50509813-50509832 Msa1253190:three_prime_UTR 30.0%
GCATCTTCTTCAAACCAATA+TGG + chr8_2:50508747-50508766 Msa1253190:five_prime_UTR 35.0%
ACCAATATGGTAGACTCAAT+AGG + chr8_2:50508760-50508779 Msa1253190:five_prime_UTR 35.0%
TCCTATTGAGTCTACCATAT+TGG - chr8_2:50508764-50508783 None:intergenic 35.0%
AGACTCAATAGGAAGAAAGT+TGG + chr8_2:50508771-50508790 Msa1253190:five_prime_UTR 35.0%
TTTCCTTCTTTCTTCCTTTG+TGG - chr8_2:50508802-50508821 None:intergenic 35.0%
ACAAAGGAAGAAAGAAGGAA+AGG + chr8_2:50508801-50508820 Msa1253190:five_prime_UTR 35.0%
CAAAGGAAGAAAGAAGGAAA+GGG + chr8_2:50508802-50508821 Msa1253190:five_prime_UTR 35.0%
TGCTGAGATAAAGAGGAATA+AGG + chr8_2:50509046-50509065 Msa1253190:CDS 35.0%
CAATACTCAATCCAAATGCT+TGG - chr8_2:50509299-50509318 None:intergenic 35.0%
! TGGATTGAGTATTGCAACAT+TGG + chr8_2:50509305-50509324 Msa1253190:CDS 35.0%
AGTAGTAGGCATCTTAAACT+TGG - chr8_2:50509406-50509425 None:intergenic 35.0%
ATGTTTATCAGGCTTAGCTT+TGG + chr8_2:50509542-50509561 Msa1253190:intron 35.0%
!! CTCATTTTTGTGTCTCTTAC+TGG - chr8_2:50509916-50509935 None:intergenic 35.0%
GCATCTTCTTCAAACCAATA+TGG + chr8_2:50508747-50508766 Msa1253190:five_prime_UTR 35.0%
ACCAATATGGTAGACTCAAT+AGG + chr8_2:50508760-50508779 Msa1253190:five_prime_UTR 35.0%
TCCTATTGAGTCTACCATAT+TGG - chr8_2:50508764-50508783 None:intergenic 35.0%
AGACTCAATAGGAAGAAAGT+TGG + chr8_2:50508771-50508790 Msa1253190:five_prime_UTR 35.0%
TTTCCTTCTTTCTTCCTTTG+TGG - chr8_2:50508802-50508821 None:intergenic 35.0%
ACAAAGGAAGAAAGAAGGAA+AGG + chr8_2:50508801-50508820 Msa1253190:five_prime_UTR 35.0%
CAAAGGAAGAAAGAAGGAAA+GGG + chr8_2:50508802-50508821 Msa1253190:five_prime_UTR 35.0%
TGCTGAGATAAAGAGGAATA+AGG + chr8_2:50509046-50509065 Msa1253190:CDS 35.0%
CAATACTCAATCCAAATGCT+TGG - chr8_2:50509299-50509318 None:intergenic 35.0%
! TGGATTGAGTATTGCAACAT+TGG + chr8_2:50509305-50509324 Msa1253190:CDS 35.0%
AGTAGTAGGCATCTTAAACT+TGG - chr8_2:50509406-50509425 None:intergenic 35.0%
ATGTTTATCAGGCTTAGCTT+TGG + chr8_2:50509542-50509561 Msa1253190:intron 35.0%
!! CTCATTTTTGTGTCTCTTAC+TGG - chr8_2:50509916-50509935 None:intergenic 35.0%
GAAAGTTGGAAACACCACAA+AGG + chr8_2:50508785-50508804 Msa1253190:five_prime_UTR 40.0%
ACACCACAAAGGAAGAAAGA+AGG + chr8_2:50508796-50508815 Msa1253190:five_prime_UTR 40.0%
ACTTGATGCTATGAAGCTGT+TGG - chr8_2:50508832-50508851 None:intergenic 40.0%
! AGGAGGATGATTTTCCATGA+TGG - chr8_2:50508946-50508965 None:intergenic 40.0%
GATGAAGGAGATTAAGCCAA+GGG - chr8_2:50509015-50509034 None:intergenic 40.0%
AGATGAAGGAGATTAAGCCA+AGG - chr8_2:50509016-50509035 None:intergenic 40.0%
AGCAGCTATACACAAGATGA+AGG - chr8_2:50509030-50509049 None:intergenic 40.0%
GTATAGCTGCTGAGATAAAG+AGG + chr8_2:50509039-50509058 Msa1253190:CDS 40.0%
TATAGGAGGAGGATCTTAGA+TGG + chr8_2:50509244-50509263 Msa1253190:intron 40.0%
!! AATCCAAATGCTTGGCTTGT+TGG - chr8_2:50509291-50509310 None:intergenic 40.0%
TAAGATGCCTACTACTGCAA+AGG + chr8_2:50509410-50509429 Msa1253190:CDS 40.0%
AACAGAACCTTTGCAGTAGT+AGG - chr8_2:50509420-50509439 None:intergenic 40.0%
TATGGGAAAGGATGGTTGAA+TGG + chr8_2:50509617-50509636 Msa1253190:CDS 40.0%
!! GGTGAGTGCTTTCTAGTTAA+AGG + chr8_2:50509638-50509657 Msa1253190:CDS 40.0%
!! GAGTGCTTTCTAGTTAAAGG+AGG + chr8_2:50509641-50509660 Msa1253190:CDS 40.0%
GTTAAAGGAGGAACTTACTC+TGG + chr8_2:50509653-50509672 Msa1253190:CDS 40.0%
AAGAAAAGGGGTTTGGAAAG+TGG + chr8_2:50509820-50509839 Msa1253190:three_prime_UTR 40.0%
AGAAAAGGGGTTTGGAAAGT+GGG + chr8_2:50509821-50509840 Msa1253190:three_prime_UTR 40.0%
TTAGCCTCTACAACAACTTG+TGG - chr8_2:50509883-50509902 None:intergenic 40.0%
GAAAGTTGGAAACACCACAA+AGG + chr8_2:50508785-50508804 Msa1253190:five_prime_UTR 40.0%
ACACCACAAAGGAAGAAAGA+AGG + chr8_2:50508796-50508815 Msa1253190:five_prime_UTR 40.0%
ACTTGATGCTATGAAGCTGT+TGG - chr8_2:50508832-50508851 None:intergenic 40.0%
! AGGAGGATGATTTTCCATGA+TGG - chr8_2:50508946-50508965 None:intergenic 40.0%
GATGAAGGAGATTAAGCCAA+GGG - chr8_2:50509015-50509034 None:intergenic 40.0%
AGATGAAGGAGATTAAGCCA+AGG - chr8_2:50509016-50509035 None:intergenic 40.0%
AGCAGCTATACACAAGATGA+AGG - chr8_2:50509030-50509049 None:intergenic 40.0%
GTATAGCTGCTGAGATAAAG+AGG + chr8_2:50509039-50509058 Msa1253190:CDS 40.0%
TATAGGAGGAGGATCTTAGA+TGG + chr8_2:50509244-50509263 Msa1253190:intron 40.0%
!! AATCCAAATGCTTGGCTTGT+TGG - chr8_2:50509291-50509310 None:intergenic 40.0%
TAAGATGCCTACTACTGCAA+AGG + chr8_2:50509410-50509429 Msa1253190:CDS 40.0%
AACAGAACCTTTGCAGTAGT+AGG - chr8_2:50509420-50509439 None:intergenic 40.0%
TATGGGAAAGGATGGTTGAA+TGG + chr8_2:50509617-50509636 Msa1253190:CDS 40.0%
!! GGTGAGTGCTTTCTAGTTAA+AGG + chr8_2:50509638-50509657 Msa1253190:CDS 40.0%
!! GAGTGCTTTCTAGTTAAAGG+AGG + chr8_2:50509641-50509660 Msa1253190:CDS 40.0%
GTTAAAGGAGGAACTTACTC+TGG + chr8_2:50509653-50509672 Msa1253190:CDS 40.0%
AAGAAAAGGGGTTTGGAAAG+TGG + chr8_2:50509820-50509839 Msa1253190:three_prime_UTR 40.0%
AGAAAAGGGGTTTGGAAAGT+GGG + chr8_2:50509821-50509840 Msa1253190:three_prime_UTR 40.0%
TTAGCCTCTACAACAACTTG+TGG - chr8_2:50509883-50509902 None:intergenic 40.0%
GGAAGAAAGAAGGAAAGGGA+TGG + chr8_2:50508806-50508825 Msa1253190:five_prime_UTR 45.0%
TCCTCCTAGATGCAAGTACA+CGG + chr8_2:50508959-50508978 Msa1253190:CDS 45.0%
ATCACCGTGTACTTGCATCT+AGG - chr8_2:50508966-50508985 None:intergenic 45.0%
!! GTGTTCGGTGTTTGTGATTG+TGG + chr8_2:50509168-50509187 Msa1253190:intron 45.0%
GTGTTTGTGATTGTGGCAGT+AGG + chr8_2:50509175-50509194 Msa1253190:intron 45.0%
GAGGAGGATCTTAGATGGAA+TGG + chr8_2:50509249-50509268 Msa1253190:CDS 45.0%
TTGCCAACAAGCCAAGCATT+TGG + chr8_2:50509285-50509304 Msa1253190:CDS 45.0%
ATGAGCAGAAGACAGCTCTA+TGG + chr8_2:50509599-50509618 Msa1253190:CDS 45.0%
TGAGCAGAAGACAGCTCTAT+GGG + chr8_2:50509600-50509619 Msa1253190:CDS 45.0%
AGAAGACAGCTCTATGGGAA+AGG + chr8_2:50509605-50509624 Msa1253190:CDS 45.0%
ATGATCTTGATGTGCTTGGC+TGG - chr8_2:50509744-50509763 None:intergenic 45.0%
CTCTACAACAACTTGTGGAG+GGG - chr8_2:50509878-50509897 None:intergenic 45.0%
CCTCTACAACAACTTGTGGA+GGG - chr8_2:50509879-50509898 None:intergenic 45.0%
TTACAAACTCGTGCTTCGCA+CGG + chr8_2:50509961-50509980 Msa1253190:three_prime_UTR 45.0%
GGAAGAAAGAAGGAAAGGGA+TGG + chr8_2:50508806-50508825 Msa1253190:five_prime_UTR 45.0%
TCCTCCTAGATGCAAGTACA+CGG + chr8_2:50508959-50508978 Msa1253190:CDS 45.0%
ATCACCGTGTACTTGCATCT+AGG - chr8_2:50508966-50508985 None:intergenic 45.0%
!! GTGTTCGGTGTTTGTGATTG+TGG + chr8_2:50509168-50509187 Msa1253190:intron 45.0%
GTGTTTGTGATTGTGGCAGT+AGG + chr8_2:50509175-50509194 Msa1253190:intron 45.0%
GAGGAGGATCTTAGATGGAA+TGG + chr8_2:50509249-50509268 Msa1253190:CDS 45.0%
TTGCCAACAAGCCAAGCATT+TGG + chr8_2:50509285-50509304 Msa1253190:CDS 45.0%
ATGAGCAGAAGACAGCTCTA+TGG + chr8_2:50509599-50509618 Msa1253190:CDS 45.0%
TGAGCAGAAGACAGCTCTAT+GGG + chr8_2:50509600-50509619 Msa1253190:CDS 45.0%
AGAAGACAGCTCTATGGGAA+AGG + chr8_2:50509605-50509624 Msa1253190:CDS 45.0%
ATGATCTTGATGTGCTTGGC+TGG - chr8_2:50509744-50509763 None:intergenic 45.0%
CTCTACAACAACTTGTGGAG+GGG - chr8_2:50509878-50509897 None:intergenic 45.0%
CCTCTACAACAACTTGTGGA+GGG - chr8_2:50509879-50509898 None:intergenic 45.0%
TTACAAACTCGTGCTTCGCA+CGG + chr8_2:50509961-50509980 Msa1253190:three_prime_UTR 45.0%
ACCGTGTACTTGCATCTAGG+AGG - chr8_2:50508963-50508982 None:intergenic 50.0%
CGACGTATCAACGTCGTGTT+CGG + chr8_2:50509153-50509172 Msa1253190:intron 50.0%
GACAGCTCTATGGGAAAGGA+TGG + chr8_2:50509609-50509628 Msa1253190:CDS 50.0%
CCCTCCACAAGTTGTTGTAG+AGG + chr8_2:50509876-50509895 Msa1253190:three_prime_UTR 50.0%
GCCTCTACAACAACTTGTGG+AGG - chr8_2:50509880-50509899 None:intergenic 50.0%
TACAAACTCGTGCTTCGCAC+GGG + chr8_2:50509962-50509981 Msa1253190:three_prime_UTR 50.0%
ACCGTGTACTTGCATCTAGG+AGG - chr8_2:50508963-50508982 None:intergenic 50.0%
CGACGTATCAACGTCGTGTT+CGG + chr8_2:50509153-50509172 Msa1253190:intron 50.0%
GACAGCTCTATGGGAAAGGA+TGG + chr8_2:50509609-50509628 Msa1253190:CDS 50.0%
CCCTCCACAAGTTGTTGTAG+AGG + chr8_2:50509876-50509895 Msa1253190:three_prime_UTR 50.0%
GCCTCTACAACAACTTGTGG+AGG - chr8_2:50509880-50509899 None:intergenic 50.0%
TACAAACTCGTGCTTCGCAC+GGG + chr8_2:50509962-50509981 Msa1253190:three_prime_UTR 50.0%
CGACGTTGATACGTCGAGAC+TGG - chr8_2:50509149-50509168 None:intergenic 55.0%
CGACGTTGATACGTCGAGAC+TGG - chr8_2:50509149-50509168 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 50508739 50510048 50508739 ID=Msa1253190;Name=Msa1253190
chr8_2 mRNA 50508739 50510048 50508739 ID=Msa1253190-mRNA-1;Parent=Msa1253190;Name=Msa1253190-mRNA-1;_AED=0.05;_eAED=0.05;_QI=209|1|1|1|1|1|3|237|193
chr8_2 exon 50508739 50509067 50508739 ID=Msa1253190-mRNA-1:exon:15378;Parent=Msa1253190-mRNA-1
chr8_2 exon 50509249 50509451 50509249 ID=Msa1253190-mRNA-1:exon:15379;Parent=Msa1253190-mRNA-1
chr8_2 exon 50509553 50510048 50509553 ID=Msa1253190-mRNA-1:exon:15380;Parent=Msa1253190-mRNA-1
chr8_2 five_prime_UTR 50508739 50508947 50508739 ID=Msa1253190-mRNA-1:five_prime_utr;Parent=Msa1253190-mRNA-1
chr8_2 CDS 50508948 50509067 50508948 ID=Msa1253190-mRNA-1:cds;Parent=Msa1253190-mRNA-1
chr8_2 CDS 50509249 50509451 50509249 ID=Msa1253190-mRNA-1:cds;Parent=Msa1253190-mRNA-1
chr8_2 CDS 50509553 50509811 50509553 ID=Msa1253190-mRNA-1:cds;Parent=Msa1253190-mRNA-1
chr8_2 three_prime_UTR 50509812 50510048 50509812 ID=Msa1253190-mRNA-1:three_prime_utr;Parent=Msa1253190-mRNA-1
Gene Sequence

>Msa1253190

ATGGAAAATCATCCTCCTAGATGCAAGTACACGGTGATCTTCTTCTTCATTATAATTTCTCTCTCCCTTGGCTTAATCTCCTTCATCTTGTGTATAGCTGCTGAGATAAAGAGGAATAAGGAGGAGGATCTTAGATGGAATGGAAAGCTATGTTATTTGCCAACAAGCCAAGCATTTGGATTGAGTATTGCAACATTGGTTAGTTTATCTTTTGCACATATAATTGGAAATTATGTGTTACTAAAAAATTCTTATTCAAGATGGAAAAATATCTCCAAGTTTAAGATGCCTACTACTGCAAAGGTTCTGTTTTTGATATCTTGGCTTAGCTTTGGAATTGTAGTTATATTATTGATTGCTGCAACAAGCATGAGCAGAAGACAGCTCTATGGGAAAGGATGGTTGAATGGTGAGTGCTTTCTAGTTAAAGGAGGAACTTACTCTGGCTCAGCCATATTGATCCTTGTTACAATTGGTTCTCTAAATGGTTCAGTTTTTTCAACATTAAAATCCAGCCAAGCACATCAAGATCATAAAATACACAAATTGGATGACGCTGAACAACCACACTTTTTGTGTTAG
Protein sequence

>Msa1253190

MENHPPRCKYTVIFFFIIISLSLGLISFILCIAAEIKRNKEEDLRWNGKLCYLPTSQAFGLSIATLVSLSFAHIIGNYVLLKNSYSRWKNISKFKMPTTAKVLFLISWLSFGIVVILLIAATSMSRRQLYGKGWLNGECFLVKGGTYSGSAILILVTIGSLNGSVFSTLKSSQAHQDHKIHKLDDAEQPHFLC*