AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1260450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260450 MtrunA17_Chr4g0061861 74.797 246 15 1 1 199 4 249 2.02e-125 353
Msa1260450 MtrunA17_Chr4g0062071 71.545 246 20 2 1 199 1 243 1.86e-104 300
Msa1260450 MtrunA17_Chr4g0062041 81.081 37 7 0 153 189 1 37 4.98e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1260450 AT5G35520.1 35.000 240 99 6 1 192 1 231 3.43e-32 116

Find 70 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTGTACTGAAATTAA+TGG 0.168658 8_2:+62482621 Msa1260450:three_prime_UTR
CTCTTGCATTGAGTTGTTTC+AGG 0.169031 8_2:+62481570 Msa1260450:CDS
TGAAATTGGGGAATCAAATT+TGG 0.229151 8_2:+62480307 Msa1260450:five_prime_UTR
TCTCGTAGTCTTTCTCAATT+TGG 0.246763 8_2:+62482673 Msa1260450:intron
ATTAATGGATTTAAATTATT+CGG 0.264266 8_2:+62482636 Msa1260450:three_prime_UTR
CTGTACCTGTAATTGTTTAT+TGG 0.268964 8_2:+62482576 Msa1260450:three_prime_UTR
AAGGATTGACAACAATAAAA+TGG 0.285180 8_2:+62482320 Msa1260450:CDS
TTCCAGCAAGGTGATGAATC+TGG 0.339575 8_2:+62479776 Msa1260450:five_prime_UTR
TGAAGTGCCTGACAAGTTAC+AGG 0.365071 8_2:+62482743 Msa1260450:three_prime_UTR
GACTACCAATAAACAATTAC+AGG 0.370178 8_2:-62482581 None:intergenic
ACCCAGATTCATCACCTTGC+TGG 0.380019 8_2:-62479778 None:intergenic
AATTGTTAGCCGAGTTTACT+AGG 0.381196 8_2:+62482802 Msa1260450:three_prime_UTR
TTTATTGGTAGTCTATTAGT+TGG 0.385190 8_2:+62482591 Msa1260450:three_prime_UTR
AGTGATAAGACCAGTCCAAC+TGG 0.389779 8_2:-62482545 None:intergenic
CAGGAAAAGTGAAAGCAAAA+AGG 0.393871 8_2:+62482301 Msa1260450:CDS
GGAATCAAATTTGGCGGGAA+TGG 0.406021 8_2:+62480316 Msa1260450:exon
TCCTGGTTCTACTTCCGATC+TGG 0.415979 8_2:+62481285 Msa1260450:CDS
AAGAAAGAGGCACATGCATT+AGG 0.422021 8_2:+62480278 Msa1260450:five_prime_UTR
CTCAGAACTTCGAATGAAGA+TGG 0.446442 8_2:+62482248 Msa1260450:CDS
TCCTGTCTCTTCAATCAAGT+TGG 0.449465 8_2:-62482283 None:intergenic
AGTAGCTTCCGCATTGAGCT+TGG 0.467482 8_2:-62480565 None:intergenic
CACAAAAGGTGAATATGTAA+AGG 0.468134 8_2:+62482898 Msa1260450:three_prime_UTR
GTAATGCCTCATTGATGTAC+TGG 0.468420 8_2:-62481531 None:intergenic
TCTTGCATTGAGTTGTTTCA+GGG 0.469644 8_2:+62481571 Msa1260450:CDS
GTCCCACCTAGATCTGTCTC+TGG 0.470999 8_2:-62479857 None:intergenic
GCTAGAAGTTTGTAAGTGAT+AGG 0.472429 8_2:+62482712 Msa1260450:three_prime_UTR
TCCAGCAAGGTGATGAATCT+GGG 0.475952 8_2:+62479777 Msa1260450:five_prime_UTR
ATGAGGGAGGGAAGCAAAGA+AGG 0.479885 8_2:+62479819 Msa1260450:five_prime_UTR
ACAAAAGGTGAATATGTAAA+GGG 0.486942 8_2:+62482899 Msa1260450:three_prime_UTR
TCAAATTTGGCGGGAATGGA+AGG 0.492575 8_2:+62480320 Msa1260450:exon
TTTCATCAACCCAGTTGGAC+TGG 0.493434 8_2:+62482535 Msa1260450:three_prime_UTR
ATGAAACCTGTGTGAGATGT+TGG 0.501041 8_2:-62480596 None:intergenic
TAGCCGAGTTTACTAGGTTG+TGG 0.504832 8_2:+62482808 Msa1260450:three_prime_UTR
GTAAATCCAGAGACAGATCT+AGG 0.507071 8_2:+62479851 Msa1260450:five_prime_UTR
GTATCTCCCTGTAACTTGTC+AGG 0.509059 8_2:-62482750 None:intergenic
ACTTGTCAGGCACTTCACTT+TGG 0.510637 8_2:-62482737 None:intergenic
AGCTTCCGCATTGAGCTTGG+TGG 0.510752 8_2:-62480562 None:intergenic
AATTGGGGAATCAAATTTGG+CGG 0.519703 8_2:+62480310 Msa1260450:five_prime_UTR
TGAAACCTGTGTGAGATGTT+GGG 0.524390 8_2:-62480595 None:intergenic
ATATGTAAAGGGAAAGTTGA+AGG 0.528998 8_2:+62482910 Msa1260450:three_prime_UTR
ATTGGGGAATCAAATTTGGC+GGG 0.534605 8_2:+62480311 Msa1260450:five_prime_UTR
ACTTCGTTGAAGAAGAGTTG+TGG 0.543092 8_2:-62480395 None:intergenic
CATCTTCATTCGAAGTTCTG+AGG 0.545490 8_2:-62482247 None:intergenic
TTCTCAATTTGGTTGTGCAA+AGG 0.550792 8_2:+62482684 Msa1260450:three_prime_UTR
AATGAGAATGATGTTGATCC+TGG 0.559856 8_2:+62481268 Msa1260450:intron
TCCAGATCGGAAGTAGAACC+AGG 0.560356 8_2:-62481286 None:intergenic
AGGGAAGCAAAGAAGGAAGC+TGG 0.561781 8_2:+62479826 Msa1260450:five_prime_UTR
ACCAACTTGATTGAAGAGAC+AGG 0.562272 8_2:+62482282 Msa1260450:CDS
CTGATGATGTTCATGAGGGA+GGG 0.563195 8_2:+62479807 Msa1260450:five_prime_UTR
TCAATTTAATATTTCCAGCA+AGG 0.565314 8_2:+62479764 Msa1260450:five_prime_UTR
TATGTAAAGGGAAAGTTGAA+GGG 0.566373 8_2:+62482911 Msa1260450:three_prime_UTR
AACAGAGGAAGTGAAGAAAG+AGG 0.576758 8_2:+62480265 Msa1260450:intron
TCAGAACTTCGAATGAAGAT+GGG 0.584789 8_2:+62482249 Msa1260450:CDS
CATTAATTTCAGTACAAGAG+AGG 0.588675 8_2:-62482620 None:intergenic
AAGAAATTCATGCATTAGAG+AGG 0.592371 8_2:+62481491 Msa1260450:CDS
TACCACTGATGATGTTCATG+AGG 0.593586 8_2:+62479802 Msa1260450:five_prime_UTR
ACTGATGATGTTCATGAGGG+AGG 0.615308 8_2:+62479806 Msa1260450:five_prime_UTR
CTGCGCATCTAATTCCAGAT+CGG 0.617171 8_2:-62481299 None:intergenic
TCTGCTATATATGCTTCCAA+AGG 0.618473 8_2:+62482348 Msa1260450:CDS
TTACCACAACCTAGTAAACT+CGG 0.619292 8_2:-62482811 None:intergenic
GTGATAAGACCAGTCCAACT+GGG 0.626319 8_2:-62482544 None:intergenic
ACCACTGATGATGTTCATGA+GGG 0.629365 8_2:+62479803 Msa1260450:five_prime_UTR
TAATGCCTCATTGATGTACT+GGG 0.649912 8_2:-62481530 None:intergenic
AGTAGAAATCGAAAGTATCG+AGG 0.666017 8_2:-62480442 None:intergenic
GAAGTGCCTGACAAGTTACA+GGG 0.669427 8_2:+62482744 Msa1260450:three_prime_UTR
TCCCTCATGAACATCATCAG+TGG 0.674652 8_2:-62479804 None:intergenic
CAATGCCCAGTACATCAATG+AGG 0.688342 8_2:+62481525 Msa1260450:CDS
AAGATCCCAACATCTCACAC+AGG 0.719465 8_2:+62480590 Msa1260450:CDS
AGCATCCACCAAGCTCAATG+CGG 0.721723 8_2:+62480557 Msa1260450:CDS
ATGCGCAGCTAAAATCACTG+AGG 0.773979 8_2:+62481314 Msa1260450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTAATTAACTTTAATT+AGG + chr8_2:62480655-62480674 Msa1260450:intron 10.0%
!!! ATTAATGGATTTAAATTATT+CGG + chr8_2:62482636-62482655 Msa1260450:three_prime_UTR 10.0%
!! ATAAGATTACTCTTTAAATT+TGG - chr8_2:62480030-62480049 None:intergenic 15.0%
!! TATTGTATAAAACTTTACAT+AGG + chr8_2:62480076-62480095 Msa1260450:intron 15.0%
!!! AGAATTTATTTCTAAAAAAG+TGG + chr8_2:62480111-62480130 Msa1260450:intron 15.0%
!!! GAATTTATTTCTAAAAAAGT+GGG + chr8_2:62480112-62480131 Msa1260450:intron 15.0%
!!! TTTTTTAATAATCTTCGTAT+GGG - chr8_2:62480229-62480248 None:intergenic 15.0%
!! CTTAAGAAAAAATTATACAA+AGG - chr8_2:62481220-62481239 None:intergenic 15.0%
!!! AATAGTTTTATCACAATATA+GGG - chr8_2:62482839-62482858 None:intergenic 15.0%
!!! AAATAGTTTTATCACAATAT+AGG - chr8_2:62482840-62482859 None:intergenic 15.0%
!! ATTTAACTGAAAGATAATGT+TGG - chr8_2:62479909-62479928 None:intergenic 20.0%
!!! CTTTTTTAATAATCTTCGTA+TGG - chr8_2:62480230-62480249 None:intergenic 20.0%
!! CGAAGATTATTAAAAAAGTT+TGG + chr8_2:62480232-62480251 Msa1260450:intron 20.0%
!! TTATTAAAAAAGTTTGGTTG+TGG + chr8_2:62480238-62480257 Msa1260450:intron 20.0%
!!! AAATTAAATCTTGTTGTTTC+AGG + chr8_2:62480532-62480551 Msa1260450:intron 20.0%
!!! AATTAAATCTTGTTGTTTCA+GGG + chr8_2:62480533-62480552 Msa1260450:intron 20.0%
!! TAAGCAATTTCTTTGAATAA+TGG - chr8_2:62480632-62480651 None:intergenic 20.0%
!! AATTGTATTGTTTGATATCT+TGG - chr8_2:62481066-62481085 None:intergenic 20.0%
!! AAAAATTATACAAAGGAACA+AGG - chr8_2:62481213-62481232 None:intergenic 20.0%
!! AATCAAATGAGAAACAAAAT+AGG - chr8_2:62481417-62481436 None:intergenic 20.0%
!! ATTTCTATTTAATCAATCCT+GGG + chr8_2:62481691-62481710 Msa1260450:intron 20.0%
!! CAAAAGAACATTGAATTATT+TGG + chr8_2:62481794-62481813 Msa1260450:intron 20.0%
!! AACATTGAATTATTTGGATA+CGG + chr8_2:62481800-62481819 Msa1260450:intron 20.0%
!!! TAAGATAAGATTTGTTTGAA+AGG + chr8_2:62481970-62481989 Msa1260450:intron 20.0%
!!! AAGATAAGATTTGTTTGAAA+GGG + chr8_2:62481971-62481990 Msa1260450:intron 20.0%
!! AAATAATGTCAAAAATTTCC+TGG + chr8_2:62482076-62482095 Msa1260450:intron 20.0%
! TCAATTTAATATTTCCAGCA+AGG + chr8_2:62479764-62479783 Msa1260450:five_prime_UTR 25.0%
! ATTAGAGAGTAAATATTAGC+CGG + chr8_2:62480153-62480172 Msa1260450:intron 25.0%
!! ATCTTTTCTAACACATATTC+CGG - chr8_2:62480175-62480194 None:intergenic 25.0%
!!! CATTAGGTTGTTTTGAAATT+GGG + chr8_2:62480294-62480313 Msa1260450:five_prime_UTR 25.0%
!!! ATTAGGTTGTTTTGAAATTG+GGG + chr8_2:62480295-62480314 Msa1260450:five_prime_UTR 25.0%
!! CAATTTCTTTGAATAATGGA+AGG - chr8_2:62480628-62480647 None:intergenic 25.0%
!!! TGATTATTTGTTTCTGTGAA+AGG + chr8_2:62480705-62480724 Msa1260450:intron 25.0%
!!! GATTATTTGTTTCTGTGAAA+GGG + chr8_2:62480706-62480725 Msa1260450:intron 25.0%
! GAAGAGAAAAACAATGATAA+AGG - chr8_2:62480801-62480820 None:intergenic 25.0%
! CCTTTAATCTCGTTAAAAAA+AGG - chr8_2:62480991-62481010 None:intergenic 25.0%
!!! CCTTTTTTTAACGAGATTAA+AGG + chr8_2:62480988-62481007 Msa1260450:intron 25.0%
! TTAAAATGCATGATATGATG+TGG + chr8_2:62481108-62481127 Msa1260450:intron 25.0%
!! GTTTCTCATTTGATTTATCT+TGG + chr8_2:62481422-62481441 Msa1260450:intron 25.0%
! GATTTCTATTTAATCAATCC+TGG + chr8_2:62481690-62481709 Msa1260450:intron 25.0%
! TATAGTTAATGCATACAAAC+AGG - chr8_2:62481733-62481752 None:intergenic 25.0%
!! TATATTACTGACTGTGTAAT+TGG - chr8_2:62481840-62481859 None:intergenic 25.0%
!! AGATAAGATTTGTTTGAAAG+GGG + chr8_2:62481972-62481991 Msa1260450:intron 25.0%
!! GTTTCATTTCTCTTTTACTA+AGG - chr8_2:62482056-62482075 None:intergenic 25.0%
! AAGGATTGACAACAATAAAA+TGG + chr8_2:62482320-62482339 Msa1260450:CDS 25.0%
!! CAACATGAATTATTTTCACA+AGG + chr8_2:62482397-62482416 Msa1260450:intron 25.0%
! AAATGTTCAAATCAGAACTA+TGG - chr8_2:62482430-62482449 None:intergenic 25.0%
!! TTTATTGGTAGTCTATTAGT+TGG + chr8_2:62482591-62482610 Msa1260450:three_prime_UTR 25.0%
!! ATTTCTTTTTACTGCACAAA+AGG + chr8_2:62482884-62482903 Msa1260450:three_prime_UTR 25.0%
! ACAAAAGGTGAATATGTAAA+GGG + chr8_2:62482899-62482918 Msa1260450:three_prime_UTR 25.0%
!!! GCATTAGGTTGTTTTGAAAT+TGG + chr8_2:62480293-62480312 Msa1260450:five_prime_UTR 30.0%
TGAAATTGGGGAATCAAATT+TGG + chr8_2:62480307-62480326 Msa1260450:five_prime_UTR 30.0%
! TCAAAAGGTTCAATCTGTTT+TGG + chr8_2:62480744-62480763 Msa1260450:intron 30.0%
TTCGCTATTCACAAATATTG+CGG + chr8_2:62480873-62480892 Msa1260450:intron 30.0%
ACATAAAAAACCCAGATATG+CGG + chr8_2:62480946-62480965 Msa1260450:intron 30.0%
! TCTCGTTAAAAAAAGGTATG+TGG - chr8_2:62480984-62481003 None:intergenic 30.0%
!!! AGGTTTTAGATAAGAGATGA+AGG - chr8_2:62481193-62481212 None:intergenic 30.0%
! AAGAAAGTGTACTTTCTTTC+AGG + chr8_2:62481237-62481256 Msa1260450:intron 30.0%
! AGAAAGTGTACTTTCTTTCA+GGG + chr8_2:62481238-62481257 Msa1260450:intron 30.0%
AAGAAATTCATGCATTAGAG+AGG + chr8_2:62481491-62481510 Msa1260450:CDS 30.0%
AACGTTAAAACATGAGCAAA+AGG - chr8_2:62481619-62481638 None:intergenic 30.0%
TTTGCAAATAATGAACATCC+AGG + chr8_2:62481883-62481902 Msa1260450:intron 30.0%
! GATAAGATTTGTTTGAAAGG+GGG + chr8_2:62481973-62481992 Msa1260450:intron 30.0%
! AATGATATTCTGGTTACCTT+TGG - chr8_2:62482367-62482386 None:intergenic 30.0%
CAGAAGAATGAATGATATTC+TGG - chr8_2:62482377-62482396 None:intergenic 30.0%
ATTTGAACATTTCTGTATGC+AGG + chr8_2:62482438-62482457 Msa1260450:intron 30.0%
! ATATTTTTCATCAACCCAGT+TGG + chr8_2:62482530-62482549 Msa1260450:three_prime_UTR 30.0%
CTGTACCTGTAATTGTTTAT+TGG + chr8_2:62482576-62482595 Msa1260450:three_prime_UTR 30.0%
GACTACCAATAAACAATTAC+AGG - chr8_2:62482584-62482603 None:intergenic 30.0%
CATTAATTTCAGTACAAGAG+AGG - chr8_2:62482623-62482642 None:intergenic 30.0%
CTCTCTTGTACTGAAATTAA+TGG + chr8_2:62482621-62482640 Msa1260450:three_prime_UTR 30.0%
CACAAAAGGTGAATATGTAA+AGG + chr8_2:62482898-62482917 Msa1260450:three_prime_UTR 30.0%
ATATGTAAAGGGAAAGTTGA+AGG + chr8_2:62482910-62482929 Msa1260450:three_prime_UTR 30.0%
TATGTAAAGGGAAAGTTGAA+GGG + chr8_2:62482911-62482930 Msa1260450:three_prime_UTR 30.0%
AATTGGGGAATCAAATTTGG+CGG + chr8_2:62480310-62480329 Msa1260450:five_prime_UTR 35.0%
!! AGTAGAAATCGAAAGTATCG+AGG - chr8_2:62480445-62480464 None:intergenic 35.0%
AAGAAGAAGAAGAAGCGAAA+GGG - chr8_2:62480481-62480500 None:intergenic 35.0%
AAAGAAGAAGAAGAAGCGAA+AGG - chr8_2:62480482-62480501 None:intergenic 35.0%
! TGTTGATTGCATTCGTCAAA+AGG + chr8_2:62480729-62480748 Msa1260450:intron 35.0%
TTTGGCAATTGAAAACCTTC+GGG - chr8_2:62480823-62480842 None:intergenic 35.0%
GTTTGGCAATTGAAAACCTT+CGG - chr8_2:62480824-62480843 None:intergenic 35.0%
!! GAACATACATTGAGAGTGTT+TGG - chr8_2:62480841-62480860 None:intergenic 35.0%
ACAAATATTGCGGAGAATCA+TGG + chr8_2:62480883-62480902 Msa1260450:intron 35.0%
CTGTAATGCAACTTTGATTG+AGG - chr8_2:62480918-62480937 None:intergenic 35.0%
ACGAGATTAAAGGAAATGCA+TGG + chr8_2:62480998-62481017 Msa1260450:intron 35.0%
ATATCTTGGCAAGTAGTGAT+TGG - chr8_2:62481052-62481071 None:intergenic 35.0%
AATGAGAATGATGTTGATCC+TGG + chr8_2:62481268-62481287 Msa1260450:intron 35.0%
AGTCTTCATTCTGTAGATAG+TGG + chr8_2:62481383-62481402 Msa1260450:intron 35.0%
! TTGGTAATAAATGTGCTGTC+AGG + chr8_2:62481441-62481460 Msa1260450:intron 35.0%
TAATAAATGTGCTGTCAGGT+GGG + chr8_2:62481445-62481464 Msa1260450:intron 35.0%
TAATGCCTCATTGATGTACT+GGG - chr8_2:62481533-62481552 None:intergenic 35.0%
! TCTTGCATTGAGTTGTTTCA+GGG + chr8_2:62481571-62481590 Msa1260450:CDS 35.0%
CATACAAACAGGTACCAAAA+TGG - chr8_2:62481722-62481741 None:intergenic 35.0%
AATCAAAAGACAGACTATCC+TGG - chr8_2:62481904-62481923 None:intergenic 35.0%
AGTATTAACATCCTGACCTA+TGG - chr8_2:62482028-62482047 None:intergenic 35.0%
TCAGAACTTCGAATGAAGAT+GGG + chr8_2:62482249-62482268 Msa1260450:CDS 35.0%
CAGGAAAAGTGAAAGCAAAA+AGG + chr8_2:62482301-62482320 Msa1260450:CDS 35.0%
TCTGCTATATATGCTTCCAA+AGG + chr8_2:62482348-62482367 Msa1260450:CDS 35.0%
TCTCGTAGTCTTTCTCAATT+TGG + chr8_2:62482673-62482692 Msa1260450:intron 35.0%
TTCTCAATTTGGTTGTGCAA+AGG + chr8_2:62482684-62482703 Msa1260450:three_prime_UTR 35.0%
GCTAGAAGTTTGTAAGTGAT+AGG + chr8_2:62482712-62482731 Msa1260450:three_prime_UTR 35.0%
AATTGTTAGCCGAGTTTACT+AGG + chr8_2:62482802-62482821 Msa1260450:three_prime_UTR 35.0%
TTACCACAACCTAGTAAACT+CGG - chr8_2:62482814-62482833 None:intergenic 35.0%
! TACCACTGATGATGTTCATG+AGG + chr8_2:62479802-62479821 Msa1260450:five_prime_UTR 40.0%
! ACCACTGATGATGTTCATGA+GGG + chr8_2:62479803-62479822 Msa1260450:five_prime_UTR 40.0%
GTAAATCCAGAGACAGATCT+AGG + chr8_2:62479851-62479870 Msa1260450:five_prime_UTR 40.0%
TTTATACACTCTCCTACCGT+AGG + chr8_2:62479972-62479991 Msa1260450:intron 40.0%
AATTTGGCAGCATTTACCGT+AGG - chr8_2:62480014-62480033 None:intergenic 40.0%
TTTGGTTGTGGTAGTAACAG+AGG + chr8_2:62480250-62480269 Msa1260450:intron 40.0%
AACAGAGGAAGTGAAGAAAG+AGG + chr8_2:62480265-62480284 Msa1260450:intron 40.0%
! AAGAAAGAGGCACATGCATT+AGG + chr8_2:62480278-62480297 Msa1260450:five_prime_UTR 40.0%
ATTGGGGAATCAAATTTGGC+GGG + chr8_2:62480311-62480330 Msa1260450:five_prime_UTR 40.0%
ACTTCGTTGAAGAAGAGTTG+TGG - chr8_2:62480398-62480417 None:intergenic 40.0%
AGAAGAAGAAGAAGCGAAAG+GGG - chr8_2:62480480-62480499 None:intergenic 40.0%
TGAAACCTGTGTGAGATGTT+GGG - chr8_2:62480598-62480617 None:intergenic 40.0%
ATGAAACCTGTGTGAGATGT+TGG - chr8_2:62480599-62480618 None:intergenic 40.0%
!! CATTGTTTTTCTCTTCCCGA+AGG + chr8_2:62480805-62480824 Msa1260450:intron 40.0%
ACTGAGGAAAAAACTAGCTG+AGG + chr8_2:62481330-62481349 Msa1260450:CDS 40.0%
GTAATAAATGTGCTGTCAGG+TGG + chr8_2:62481444-62481463 Msa1260450:intron 40.0%
AATAAATGTGCTGTCAGGTG+GGG + chr8_2:62481446-62481465 Msa1260450:intron 40.0%
GTAATGCCTCATTGATGTAC+TGG - chr8_2:62481534-62481553 None:intergenic 40.0%
! CTCTTGCATTGAGTTGTTTC+AGG + chr8_2:62481570-62481589 Msa1260450:CDS 40.0%
!! ATACTAGTTCTGAGTAGCAC+AGG - chr8_2:62481655-62481674 None:intergenic 40.0%
! AATCCTGGGATTCTCCATTT+TGG + chr8_2:62481705-62481724 Msa1260450:intron 40.0%
CTGACCTATGGTACCTTTAT+TGG - chr8_2:62482016-62482035 None:intergenic 40.0%
!! CAATAAAGGTACCATAGGTC+AGG + chr8_2:62482014-62482033 Msa1260450:intron 40.0%
CATCTTCATTCGAAGTTCTG+AGG - chr8_2:62482250-62482269 None:intergenic 40.0%
CTCAGAACTTCGAATGAAGA+TGG + chr8_2:62482248-62482267 Msa1260450:CDS 40.0%
ACCAACTTGATTGAAGAGAC+AGG + chr8_2:62482282-62482301 Msa1260450:CDS 40.0%
TCCTGTCTCTTCAATCAAGT+TGG - chr8_2:62482286-62482305 None:intergenic 40.0%
GAAAGACTACGAGATTCTTC+AGG - chr8_2:62482667-62482686 None:intergenic 40.0%
TTCCAGCAAGGTGATGAATC+TGG + chr8_2:62479776-62479795 Msa1260450:five_prime_UTR 45.0%
TCCAGCAAGGTGATGAATCT+GGG + chr8_2:62479777-62479796 Msa1260450:five_prime_UTR 45.0%
TCCCTCATGAACATCATCAG+TGG - chr8_2:62479807-62479826 None:intergenic 45.0%
! ACTGATGATGTTCATGAGGG+AGG + chr8_2:62479806-62479825 Msa1260450:five_prime_UTR 45.0%
! CTGATGATGTTCATGAGGGA+GGG + chr8_2:62479807-62479826 Msa1260450:five_prime_UTR 45.0%
AATCCAGAGACAGATCTAGG+TGG + chr8_2:62479854-62479873 Msa1260450:intron 45.0%
ATCCAGAGACAGATCTAGGT+GGG + chr8_2:62479855-62479874 Msa1260450:intron 45.0%
GGAATCAAATTTGGCGGGAA+TGG + chr8_2:62480316-62480335 Msa1260450:exon 45.0%
TCAAATTTGGCGGGAATGGA+AGG + chr8_2:62480320-62480339 Msa1260450:exon 45.0%
!! AAGCGAAAGGGGTTAGTTAC+TGG - chr8_2:62480469-62480488 None:intergenic 45.0%
AAGATCCCAACATCTCACAC+AGG + chr8_2:62480590-62480609 Msa1260450:CDS 45.0%
TGGATCAAAAGCTCGCTGTT+TGG + chr8_2:62480764-62480783 Msa1260450:intron 45.0%
GGATCAAAAGCTCGCTGTTT+GGG + chr8_2:62480765-62480784 Msa1260450:intron 45.0%
CTGCGCATCTAATTCCAGAT+CGG - chr8_2:62481302-62481321 None:intergenic 45.0%
ATGCGCAGCTAAAATCACTG+AGG + chr8_2:62481314-62481333 Msa1260450:CDS 45.0%
CAATGCCCAGTACATCAATG+AGG + chr8_2:62481525-62481544 Msa1260450:CDS 45.0%
GTACCAAAATGGAGAATCCC+AGG - chr8_2:62481711-62481730 None:intergenic 45.0%
! TTGAAAGGGGGCCAACATTT+TGG + chr8_2:62481985-62482004 Msa1260450:intron 45.0%
! TGAAAGGGGGCCAACATTTT+GGG + chr8_2:62481986-62482005 Msa1260450:intron 45.0%
! TATTGGTGCCCCCAAAATGT+TGG - chr8_2:62481999-62482018 None:intergenic 45.0%
!!! CATTTTGGGGGCACCAATAA+AGG + chr8_2:62482000-62482019 Msa1260450:intron 45.0%
!! GGCACCAATAAAGGTACCAT+AGG + chr8_2:62482009-62482028 Msa1260450:intron 45.0%
AGCTCTGCACTATTGCAAGT+AGG + chr8_2:62482099-62482118 Msa1260450:intron 45.0%
GCAGTTGTCATGATCTCTAC+AGG - chr8_2:62482228-62482247 None:intergenic 45.0%
TTTCATCAACCCAGTTGGAC+TGG + chr8_2:62482535-62482554 Msa1260450:three_prime_UTR 45.0%
GTGATAAGACCAGTCCAACT+GGG - chr8_2:62482547-62482566 None:intergenic 45.0%
AGTGATAAGACCAGTCCAAC+TGG - chr8_2:62482548-62482567 None:intergenic 45.0%
ACTTGTCAGGCACTTCACTT+TGG - chr8_2:62482740-62482759 None:intergenic 45.0%
! TGAAGTGCCTGACAAGTTAC+AGG + chr8_2:62482743-62482762 Msa1260450:three_prime_UTR 45.0%
! GAAGTGCCTGACAAGTTACA+GGG + chr8_2:62482744-62482763 Msa1260450:three_prime_UTR 45.0%
! GTATCTCCCTGTAACTTGTC+AGG - chr8_2:62482753-62482772 None:intergenic 45.0%
TAGCCGAGTTTACTAGGTTG+TGG + chr8_2:62482808-62482827 Msa1260450:three_prime_UTR 45.0%
!! AATTTAATTAACTTTAATTA+GGG + chr8_2:62480656-62480675 Msa1260450:intron 5.0%
ACCCAGATTCATCACCTTGC+TGG - chr8_2:62479781-62479800 None:intergenic 50.0%
ATGAGGGAGGGAAGCAAAGA+AGG + chr8_2:62479819-62479838 Msa1260450:five_prime_UTR 50.0%
AGGGAAGCAAAGAAGGAAGC+TGG + chr8_2:62479826-62479845 Msa1260450:five_prime_UTR 50.0%
AGAGGGGAATAGGTCTCCTA+CGG - chr8_2:62479991-62480010 None:intergenic 50.0%
AGACCTATTCCCCTCTCCTA+CGG + chr8_2:62479995-62480014 Msa1260450:intron 50.0%
TTACCGTAGGAGAGGGGAAT+AGG - chr8_2:62480001-62480020 None:intergenic 50.0%
CAGCATTTACCGTAGGAGAG+GGG - chr8_2:62480007-62480026 None:intergenic 50.0%
GCAGCATTTACCGTAGGAGA+GGG - chr8_2:62480008-62480027 None:intergenic 50.0%
!! GAGCGACGCTGTTTTCGAAT+CGG + chr8_2:62480358-62480377 Msa1260450:CDS 50.0%
AGCATCCACCAAGCTCAATG+CGG + chr8_2:62480557-62480576 Msa1260450:CDS 50.0%
AGTAGCTTCCGCATTGAGCT+TGG - chr8_2:62480568-62480587 None:intergenic 50.0%
GCAATCTAGGCCGCATATCT+GGG - chr8_2:62480959-62480978 None:intergenic 50.0%
CAGATATGCGGCCTAGATTG+CGG + chr8_2:62480958-62480977 Msa1260450:intron 50.0%
AAGGTATGTGGCCGCAATCT+AGG - chr8_2:62480972-62480991 None:intergenic 50.0%
!! TCCTGGTTCTACTTCCGATC+TGG + chr8_2:62481285-62481304 Msa1260450:CDS 50.0%
TCCAGATCGGAAGTAGAACC+AGG - chr8_2:62481289-62481308 None:intergenic 50.0%
! GAAAGGGGGCCAACATTTTG+GGG + chr8_2:62481987-62482006 Msa1260450:intron 50.0%
! AAAGGGGGCCAACATTTTGG+GGG + chr8_2:62481988-62482007 Msa1260450:intron 50.0%
TTGCAATAGTGCAGAGCTCC+AGG - chr8_2:62482097-62482116 None:intergenic 50.0%
GTCCCACCTAGATCTGTCTC+TGG - chr8_2:62479860-62479879 None:intergenic 55.0%
GGGAATAGGTCTCCTACGGT+AGG - chr8_2:62479987-62480006 None:intergenic 55.0%
GGCAGCATTTACCGTAGGAG+AGG - chr8_2:62480009-62480028 None:intergenic 55.0%
AGCTTCCGCATTGAGCTTGG+TGG - chr8_2:62480565-62480584 None:intergenic 55.0%
GCGGAGAATCATGGTCAACG+TGG + chr8_2:62480892-62480911 Msa1260450:intron 55.0%
CGCAATCTAGGCCGCATATC+TGG - chr8_2:62480960-62480979 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 62479742 62482941 62479742 ID=Msa1260450;Name=Msa1260450
chr8_2 mRNA 62479742 62482941 62479742 ID=Msa1260450-mRNA-1;Parent=Msa1260450;Name=Msa1260450-mRNA-1;_AED=0.13;_eAED=0.13;_QI=196|0.87|0.88|1|0.62|0.55|9|375|199
chr8_2 exon 62479742 62479872 62479742 ID=Msa1260450-mRNA-1:exon:19792;Parent=Msa1260450-mRNA-1
chr8_2 exon 62480270 62480468 62480270 ID=Msa1260450-mRNA-1:exon:19793;Parent=Msa1260450-mRNA-1
chr8_2 exon 62480554 62480611 62480554 ID=Msa1260450-mRNA-1:exon:19794;Parent=Msa1260450-mRNA-1
chr8_2 exon 62481274 62481351 62481274 ID=Msa1260450-mRNA-1:exon:19795;Parent=Msa1260450-mRNA-1
chr8_2 exon 62481463 62481592 62481463 ID=Msa1260450-mRNA-1:exon:19796;Parent=Msa1260450-mRNA-1
chr8_2 exon 62482232 62482369 62482232 ID=Msa1260450-mRNA-1:exon:19797;Parent=Msa1260450-mRNA-1
chr8_2 exon 62482460 62482658 62482460 ID=Msa1260450-mRNA-1:exon:19798;Parent=Msa1260450-mRNA-1
chr8_2 exon 62482681 62482847 62482681 ID=Msa1260450-mRNA-1:exon:19799;Parent=Msa1260450-mRNA-1
chr8_2 exon 62482871 62482941 62482871 ID=Msa1260450-mRNA-1:exon:19800;Parent=Msa1260450-mRNA-1
chr8_2 five_prime_UTR 62479742 62479872 62479742 ID=Msa1260450-mRNA-1:five_prime_utr;Parent=Msa1260450-mRNA-1
chr8_2 five_prime_UTR 62480270 62480334 62480270 ID=Msa1260450-mRNA-1:five_prime_utr;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62480335 62480468 62480335 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62480554 62480611 62480554 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62481274 62481351 62481274 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62481463 62481592 62481463 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62482232 62482369 62482232 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 CDS 62482460 62482521 62482460 ID=Msa1260450-mRNA-1:cds;Parent=Msa1260450-mRNA-1
chr8_2 three_prime_UTR 62482522 62482658 62482522 ID=Msa1260450-mRNA-1:three_prime_utr;Parent=Msa1260450-mRNA-1
chr8_2 three_prime_UTR 62482681 62482847 62482681 ID=Msa1260450-mRNA-1:three_prime_utr;Parent=Msa1260450-mRNA-1
chr8_2 three_prime_UTR 62482871 62482941 62482871 ID=Msa1260450-mRNA-1:three_prime_utr;Parent=Msa1260450-mRNA-1
Gene Sequence

>Msa1260450

ATGGAAGGAAGCATGAGCAGAGAGAGCGACGCTGTTTTCGAATCGGTGATGAATCTGAATCCACAACTCTTCTTCAACGAAGTTCTCAACACTGTTGATGATTTCGTCCTCGATACTTTCGATTTCTACTTCCAGGAAGCATCCACCAAGCTCAATGCGGAAGCTACTCAAAGATCCCAACATCTCACACAGAATGATGTTGATCCTGGTTCTACTTCCGATCTGGAATTAGATGCGCAGCTAAAATCACTGAGGAAAAAACTAGCTGAGGTGGGGAAAGAGTCTGAAACGCTGAATCAAGAAATTCATGCATTAGAGAGGCAGTCTTCTCACAATGCCCAGTACATCAATGAGGCATTACAATTATTTGAGCAAAACTCTTGCATTGAGTTGTTTCAGGAGATCATGACAACTGCCTCAGAACTTCGAATGAAGATGGGAAAGCTTAACACCAACTTGATTGAAGAGACAGGAAAAGTGAAAGCAAAAAGGATTGACAACAATAAAATGGACATTTCTGCTATATATGCTTCCAAAGGCGTATCTAATACGAAATTCGAAGATCTTCAAGAATTTGTAACTCTTATGAAGAGCACGTGA
Protein sequence

>Msa1260450

MEGSMSRESDAVFESVMNLNPQLFFNEVLNTVDDFVLDTFDFYFQEASTKLNAEATQRSQHLTQNDVDPGSTSDLELDAQLKSLRKKLAEVGKESETLNQEIHALERQSSHNAQYINEALQLFEQNSCIELFQEIMTTASELRMKMGKLNTNLIEETGKVKAKRIDNNKMDISAIYASKGVSNTKFEDLQEFVTLMKST*