AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1300290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300290 MtrunA17_Chr4g0061861 74.797 246 15 1 1 199 4 249 2.02e-125 353
Msa1300290 MtrunA17_Chr4g0062071 71.545 246 20 2 1 199 1 243 1.86e-104 300
Msa1300290 MtrunA17_Chr4g0062041 81.081 37 7 0 153 189 1 37 4.98e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1300290 AT5G35520.1 35.000 240 99 6 1 192 1 231 3.43e-32 116

Find 70 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTGTACTGAAATTAA+TGG 0.168658 8_3:+61831706 Msa1300290:three_prime_UTR
CTCTTGCATTGAGTTGTTTC+AGG 0.169031 8_3:+61830655 Msa1300290:CDS
TGAAATTGGGGAATCAAATT+TGG 0.229151 8_3:+61829386 Msa1300290:five_prime_UTR
TCTCGTAGTCTTTCTCAATT+TGG 0.246763 8_3:+61831758 Msa1300290:intron
ATTAATGGATTTAAATTATT+CGG 0.264266 8_3:+61831721 Msa1300290:three_prime_UTR
CTGTACCTGTAATTGTTTAT+TGG 0.268964 8_3:+61831661 Msa1300290:three_prime_UTR
AAGGATTGACAACAATAAAA+TGG 0.285180 8_3:+61831405 Msa1300290:CDS
TTCCAGCAAGGTGATGAATC+TGG 0.339575 8_3:+61828856 Msa1300290:five_prime_UTR
TGAAGTGCCTGACAAGTTAC+AGG 0.365071 8_3:+61831828 Msa1300290:three_prime_UTR
GACTACCAATAAACAATTAC+AGG 0.370178 8_3:-61831666 None:intergenic
ACCCAGATTCATCACCTTGC+TGG 0.380019 8_3:-61828858 None:intergenic
AATTGTTAGCCGAGTTTACT+AGG 0.381196 8_3:+61831887 Msa1300290:three_prime_UTR
TTTATTGGTAGTCTATTAGT+TGG 0.385190 8_3:+61831676 Msa1300290:three_prime_UTR
AGTGATAAGACCAGTCCAAC+TGG 0.389779 8_3:-61831630 None:intergenic
CAGGAAAAGTGAAAGCAAAA+AGG 0.393871 8_3:+61831386 Msa1300290:CDS
GGAATCAAATTTGGCGGGAA+TGG 0.406021 8_3:+61829395 Msa1300290:exon
TCCTGGTTCTACTTCCGATC+TGG 0.415979 8_3:+61830370 Msa1300290:CDS
AAGAAAGAGGCACATGCATT+AGG 0.422021 8_3:+61829357 Msa1300290:five_prime_UTR
CTCAGAACTTCGAATGAAGA+TGG 0.446442 8_3:+61831333 Msa1300290:CDS
TCCTGTCTCTTCAATCAAGT+TGG 0.449465 8_3:-61831368 None:intergenic
AGTAGCTTCCGCATTGAGCT+TGG 0.467482 8_3:-61829650 None:intergenic
CACAAAAGGTGAATATGTAA+AGG 0.468134 8_3:+61831983 Msa1300290:three_prime_UTR
GTAATGCCTCATTGATGTAC+TGG 0.468420 8_3:-61830616 None:intergenic
TCTTGCATTGAGTTGTTTCA+GGG 0.469644 8_3:+61830656 Msa1300290:CDS
GTCCCACCTAGATCTGTCTC+TGG 0.470999 8_3:-61828937 None:intergenic
GCTAGAAGTTTGTAAGTGAT+AGG 0.472429 8_3:+61831797 Msa1300290:three_prime_UTR
TCCAGCAAGGTGATGAATCT+GGG 0.475952 8_3:+61828857 Msa1300290:five_prime_UTR
ATGAGGGAGGGAAGCAAAGA+AGG 0.479885 8_3:+61828899 Msa1300290:five_prime_UTR
ACAAAAGGTGAATATGTAAA+GGG 0.486942 8_3:+61831984 Msa1300290:three_prime_UTR
TCAAATTTGGCGGGAATGGA+AGG 0.492575 8_3:+61829399 Msa1300290:exon
TTTCATCAACCCAGTTGGAC+TGG 0.493434 8_3:+61831620 Msa1300290:three_prime_UTR
ATGAAACCTGTGTGAGATGT+TGG 0.501041 8_3:-61829681 None:intergenic
TAGCCGAGTTTACTAGGTTG+TGG 0.504832 8_3:+61831893 Msa1300290:three_prime_UTR
GTAAATCCAGAGACAGATCT+AGG 0.507071 8_3:+61828931 Msa1300290:five_prime_UTR
GTATCTCCCTGTAACTTGTC+AGG 0.509059 8_3:-61831835 None:intergenic
ACTTGTCAGGCACTTCACTT+TGG 0.510637 8_3:-61831822 None:intergenic
AGCTTCCGCATTGAGCTTGG+TGG 0.510752 8_3:-61829647 None:intergenic
AATTGGGGAATCAAATTTGG+CGG 0.519703 8_3:+61829389 Msa1300290:five_prime_UTR
TGAAACCTGTGTGAGATGTT+GGG 0.524390 8_3:-61829680 None:intergenic
ATATGTAAAGGGAAAGTTGA+AGG 0.528998 8_3:+61831995 Msa1300290:three_prime_UTR
ATTGGGGAATCAAATTTGGC+GGG 0.534605 8_3:+61829390 Msa1300290:five_prime_UTR
ACTTCGTTGAAGAAGAGTTG+TGG 0.543092 8_3:-61829474 None:intergenic
CATCTTCATTCGAAGTTCTG+AGG 0.545490 8_3:-61831332 None:intergenic
TTCTCAATTTGGTTGTGCAA+AGG 0.550792 8_3:+61831769 Msa1300290:three_prime_UTR
AATGAGAATGATGTTGATCC+TGG 0.559856 8_3:+61830353 Msa1300290:intron
TCCAGATCGGAAGTAGAACC+AGG 0.560356 8_3:-61830371 None:intergenic
AGGGAAGCAAAGAAGGAAGC+TGG 0.561781 8_3:+61828906 Msa1300290:five_prime_UTR
ACCAACTTGATTGAAGAGAC+AGG 0.562272 8_3:+61831367 Msa1300290:CDS
CTGATGATGTTCATGAGGGA+GGG 0.563195 8_3:+61828887 Msa1300290:five_prime_UTR
TCAATTTAATATTTCCAGCA+AGG 0.565314 8_3:+61828844 Msa1300290:five_prime_UTR
TATGTAAAGGGAAAGTTGAA+GGG 0.566373 8_3:+61831996 Msa1300290:three_prime_UTR
AACAGAGGAAGTGAAGAAAG+AGG 0.576758 8_3:+61829344 Msa1300290:intron
TCAGAACTTCGAATGAAGAT+GGG 0.584789 8_3:+61831334 Msa1300290:CDS
CATTAATTTCAGTACAAGAG+AGG 0.588675 8_3:-61831705 None:intergenic
AAGAAATTCATGCATTAGAG+AGG 0.592371 8_3:+61830576 Msa1300290:CDS
TACCACTGATGATGTTCATG+AGG 0.593586 8_3:+61828882 Msa1300290:five_prime_UTR
ACTGATGATGTTCATGAGGG+AGG 0.615308 8_3:+61828886 Msa1300290:five_prime_UTR
CTGCGCATCTAATTCCAGAT+CGG 0.617171 8_3:-61830384 None:intergenic
TCTGCTATATATGCTTCCAA+AGG 0.618473 8_3:+61831433 Msa1300290:CDS
TTACCACAACCTAGTAAACT+CGG 0.619292 8_3:-61831896 None:intergenic
GTGATAAGACCAGTCCAACT+GGG 0.626319 8_3:-61831629 None:intergenic
ACCACTGATGATGTTCATGA+GGG 0.629365 8_3:+61828883 Msa1300290:five_prime_UTR
TAATGCCTCATTGATGTACT+GGG 0.649912 8_3:-61830615 None:intergenic
AGTAGAAATCGAAAGTATCG+AGG 0.666017 8_3:-61829521 None:intergenic
GAAGTGCCTGACAAGTTACA+GGG 0.669427 8_3:+61831829 Msa1300290:three_prime_UTR
TCCCTCATGAACATCATCAG+TGG 0.674652 8_3:-61828884 None:intergenic
CAATGCCCAGTACATCAATG+AGG 0.688342 8_3:+61830610 Msa1300290:CDS
AAGATCCCAACATCTCACAC+AGG 0.719465 8_3:+61829675 Msa1300290:CDS
AGCATCCACCAAGCTCAATG+CGG 0.721723 8_3:+61829642 Msa1300290:CDS
ATGCGCAGCTAAAATCACTG+AGG 0.773979 8_3:+61830399 Msa1300290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTTAATTAACTTTAATT+AGG + chr8_3:61829740-61829759 Msa1300290:intron 10.0%
!!! ATTAATGGATTTAAATTATT+CGG + chr8_3:61831721-61831740 Msa1300290:three_prime_UTR 10.0%
!! TAATTTATTCATTTAACTGA+AGG - chr8_3:61828999-61829018 None:intergenic 15.0%
!! ATAAGATTACTCTTTAAATT+TGG - chr8_3:61829109-61829128 None:intergenic 15.0%
!! TATTGTATAAAACTTTACAT+AGG + chr8_3:61829155-61829174 Msa1300290:intron 15.0%
!!! AGAATTTATTTCTAAAAAAG+TGG + chr8_3:61829190-61829209 Msa1300290:intron 15.0%
!!! GAATTTATTTCTAAAAAAGT+GGG + chr8_3:61829191-61829210 Msa1300290:intron 15.0%
!!! TTTTTTAATAATCTTCGTAT+GGG - chr8_3:61829308-61829327 None:intergenic 15.0%
!! CTTAAGAAAAAATTATACAA+AGG - chr8_3:61830305-61830324 None:intergenic 15.0%
!!! AATAGTTTTATCACAATATA+GGG - chr8_3:61831924-61831943 None:intergenic 15.0%
!!! AAATAGTTTTATCACAATAT+AGG - chr8_3:61831925-61831944 None:intergenic 15.0%
!!! CTTTTTTAATAATCTTCGTA+TGG - chr8_3:61829309-61829328 None:intergenic 20.0%
!! CGAAGATTATTAAAAAAGTT+TGG + chr8_3:61829311-61829330 Msa1300290:intron 20.0%
!! TTATTAAAAAAGTTTGGTTG+TGG + chr8_3:61829317-61829336 Msa1300290:intron 20.0%
!!! AAATTAAATCTTGTTGTTTC+AGG + chr8_3:61829617-61829636 Msa1300290:intron 20.0%
!!! AATTAAATCTTGTTGTTTCA+GGG + chr8_3:61829618-61829637 Msa1300290:intron 20.0%
!! TAAGCAATTTCTTTGAATAA+TGG - chr8_3:61829717-61829736 None:intergenic 20.0%
!! AATTGTATTGTTTGATATCT+TGG - chr8_3:61830151-61830170 None:intergenic 20.0%
!! AAAAATTATACAAAGGAACA+AGG - chr8_3:61830298-61830317 None:intergenic 20.0%
!! AATCAAATGAGAAACAAAAT+AGG - chr8_3:61830502-61830521 None:intergenic 20.0%
!! ATTTCTATTTAATCAATCCT+GGG + chr8_3:61830776-61830795 Msa1300290:intron 20.0%
!! CAAAAGAACATTGAATTATT+TGG + chr8_3:61830879-61830898 Msa1300290:intron 20.0%
!! AACATTGAATTATTTGGATA+CGG + chr8_3:61830885-61830904 Msa1300290:intron 20.0%
!!! TAAGATAAGATTTGTTTGAA+AGG + chr8_3:61831055-61831074 Msa1300290:intron 20.0%
!!! AAGATAAGATTTGTTTGAAA+GGG + chr8_3:61831056-61831075 Msa1300290:intron 20.0%
!! AAATAATGTCAAAAATTTCC+TGG + chr8_3:61831161-61831180 Msa1300290:intron 20.0%
! TCAATTTAATATTTCCAGCA+AGG + chr8_3:61828844-61828863 Msa1300290:five_prime_UTR 25.0%
! ATTTAACTGAAGGATAATGT+TGG - chr8_3:61828989-61829008 None:intergenic 25.0%
! ATTAGAGAGTAAATATTAGC+CGG + chr8_3:61829232-61829251 Msa1300290:intron 25.0%
!! ATCTTTTCTAACACATATTC+CGG - chr8_3:61829254-61829273 None:intergenic 25.0%
!!! CATTAGGTTGTTTTGAAATT+GGG + chr8_3:61829373-61829392 Msa1300290:five_prime_UTR 25.0%
!!! ATTAGGTTGTTTTGAAATTG+GGG + chr8_3:61829374-61829393 Msa1300290:five_prime_UTR 25.0%
!! CAATTTCTTTGAATAATGGA+AGG - chr8_3:61829713-61829732 None:intergenic 25.0%
!!! TGATTATTTGTTTCTGTGAA+AGG + chr8_3:61829790-61829809 Msa1300290:intron 25.0%
!!! GATTATTTGTTTCTGTGAAA+GGG + chr8_3:61829791-61829810 Msa1300290:intron 25.0%
! GAAGAGAAAAACAATGATAA+AGG - chr8_3:61829886-61829905 None:intergenic 25.0%
! CCTTTAATCTCGTTAAAAAA+AGG - chr8_3:61830076-61830095 None:intergenic 25.0%
!!! CCTTTTTTTAACGAGATTAA+AGG + chr8_3:61830073-61830092 Msa1300290:intron 25.0%
! TTAAAATGCATGATATGATG+TGG + chr8_3:61830193-61830212 Msa1300290:intron 25.0%
!! GTTTCTCATTTGATTTATCT+TGG + chr8_3:61830507-61830526 Msa1300290:intron 25.0%
! GATTTCTATTTAATCAATCC+TGG + chr8_3:61830775-61830794 Msa1300290:intron 25.0%
! TATAGTTAATGCATACAAAC+AGG - chr8_3:61830818-61830837 None:intergenic 25.0%
!! TATATTACTGACTGTGTAAT+TGG - chr8_3:61830925-61830944 None:intergenic 25.0%
!! AGATAAGATTTGTTTGAAAG+GGG + chr8_3:61831057-61831076 Msa1300290:intron 25.0%
!! GTTTCATTTCTCTTTTACTA+AGG - chr8_3:61831141-61831160 None:intergenic 25.0%
! AAGGATTGACAACAATAAAA+TGG + chr8_3:61831405-61831424 Msa1300290:CDS 25.0%
!! CAACATGAATTATTTTCACA+AGG + chr8_3:61831482-61831501 Msa1300290:intron 25.0%
! AAATGTTCAAATCAGAACTA+TGG - chr8_3:61831515-61831534 None:intergenic 25.0%
!! TTTATTGGTAGTCTATTAGT+TGG + chr8_3:61831676-61831695 Msa1300290:three_prime_UTR 25.0%
!! ATTTCTTTTTACTGCACAAA+AGG + chr8_3:61831969-61831988 Msa1300290:three_prime_UTR 25.0%
! ACAAAAGGTGAATATGTAAA+GGG + chr8_3:61831984-61832003 Msa1300290:three_prime_UTR 25.0%
!!! GCATTAGGTTGTTTTGAAAT+TGG + chr8_3:61829372-61829391 Msa1300290:five_prime_UTR 30.0%
TGAAATTGGGGAATCAAATT+TGG + chr8_3:61829386-61829405 Msa1300290:five_prime_UTR 30.0%
! TCAAAAGGTTCAATCTGTTT+TGG + chr8_3:61829829-61829848 Msa1300290:intron 30.0%
TTCGCTATTCACAAATATTG+CGG + chr8_3:61829958-61829977 Msa1300290:intron 30.0%
ACATAAAAAACCCAGATATG+CGG + chr8_3:61830031-61830050 Msa1300290:intron 30.0%
! TCTCGTTAAAAAAAGGTATG+TGG - chr8_3:61830069-61830088 None:intergenic 30.0%
!!! AGGTTTTAGATAAGAGATGA+AGG - chr8_3:61830278-61830297 None:intergenic 30.0%
! AAGAAAGTGTACTTTCTTTC+AGG + chr8_3:61830322-61830341 Msa1300290:intron 30.0%
! AGAAAGTGTACTTTCTTTCA+GGG + chr8_3:61830323-61830342 Msa1300290:intron 30.0%
AAGAAATTCATGCATTAGAG+AGG + chr8_3:61830576-61830595 Msa1300290:CDS 30.0%
AACGTTAAAACATGAGCAAA+AGG - chr8_3:61830704-61830723 None:intergenic 30.0%
TTTGCAAATAATGAACATCC+AGG + chr8_3:61830968-61830987 Msa1300290:intron 30.0%
! GATAAGATTTGTTTGAAAGG+GGG + chr8_3:61831058-61831077 Msa1300290:intron 30.0%
! AATGATATTCTGGTTACCTT+TGG - chr8_3:61831452-61831471 None:intergenic 30.0%
CAGAAGAATGAATGATATTC+TGG - chr8_3:61831462-61831481 None:intergenic 30.0%
ATTTGAACATTTCTGTATGC+AGG + chr8_3:61831523-61831542 Msa1300290:intron 30.0%
! ATATTTTTCATCAACCCAGT+TGG + chr8_3:61831615-61831634 Msa1300290:three_prime_UTR 30.0%
CTGTACCTGTAATTGTTTAT+TGG + chr8_3:61831661-61831680 Msa1300290:three_prime_UTR 30.0%
GACTACCAATAAACAATTAC+AGG - chr8_3:61831669-61831688 None:intergenic 30.0%
CATTAATTTCAGTACAAGAG+AGG - chr8_3:61831708-61831727 None:intergenic 30.0%
CTCTCTTGTACTGAAATTAA+TGG + chr8_3:61831706-61831725 Msa1300290:three_prime_UTR 30.0%
CACAAAAGGTGAATATGTAA+AGG + chr8_3:61831983-61832002 Msa1300290:three_prime_UTR 30.0%
ATATGTAAAGGGAAAGTTGA+AGG + chr8_3:61831995-61832014 Msa1300290:three_prime_UTR 30.0%
TATGTAAAGGGAAAGTTGAA+GGG + chr8_3:61831996-61832015 Msa1300290:three_prime_UTR 30.0%
AATTGGGGAATCAAATTTGG+CGG + chr8_3:61829389-61829408 Msa1300290:five_prime_UTR 35.0%
!! AGTAGAAATCGAAAGTATCG+AGG - chr8_3:61829524-61829543 None:intergenic 35.0%
AAGAAGAAGAAGAAGCGAAA+GGG - chr8_3:61829560-61829579 None:intergenic 35.0%
AAAGAAGAAGAAGAAGCGAA+AGG - chr8_3:61829561-61829580 None:intergenic 35.0%
! TGTTGATTGCATTCGTCAAA+AGG + chr8_3:61829814-61829833 Msa1300290:intron 35.0%
TTTGGCAATTGAAAACCTTC+GGG - chr8_3:61829908-61829927 None:intergenic 35.0%
GTTTGGCAATTGAAAACCTT+CGG - chr8_3:61829909-61829928 None:intergenic 35.0%
!! GAACATACATTGAGAGTGTT+TGG - chr8_3:61829926-61829945 None:intergenic 35.0%
ACAAATATTGCGGAGAATCA+TGG + chr8_3:61829968-61829987 Msa1300290:intron 35.0%
CTGTAATGCAACTTTGATTG+AGG - chr8_3:61830003-61830022 None:intergenic 35.0%
ACGAGATTAAAGGAAATGCA+TGG + chr8_3:61830083-61830102 Msa1300290:intron 35.0%
ATATCTTGGCAAGTAGTGAT+TGG - chr8_3:61830137-61830156 None:intergenic 35.0%
AATGAGAATGATGTTGATCC+TGG + chr8_3:61830353-61830372 Msa1300290:intron 35.0%
AGTCTTCATTCTGTAGATAG+TGG + chr8_3:61830468-61830487 Msa1300290:intron 35.0%
! TTGGTAATAAATGTGCTGTC+AGG + chr8_3:61830526-61830545 Msa1300290:intron 35.0%
TAATAAATGTGCTGTCAGGT+GGG + chr8_3:61830530-61830549 Msa1300290:intron 35.0%
TAATGCCTCATTGATGTACT+GGG - chr8_3:61830618-61830637 None:intergenic 35.0%
! TCTTGCATTGAGTTGTTTCA+GGG + chr8_3:61830656-61830675 Msa1300290:CDS 35.0%
CATACAAACAGGTACCAAAA+TGG - chr8_3:61830807-61830826 None:intergenic 35.0%
AATCAAAAGACAGACTATCC+TGG - chr8_3:61830989-61831008 None:intergenic 35.0%
AGTATTAACATCCTGACCTA+TGG - chr8_3:61831113-61831132 None:intergenic 35.0%
TCAGAACTTCGAATGAAGAT+GGG + chr8_3:61831334-61831353 Msa1300290:CDS 35.0%
CAGGAAAAGTGAAAGCAAAA+AGG + chr8_3:61831386-61831405 Msa1300290:CDS 35.0%
TCTGCTATATATGCTTCCAA+AGG + chr8_3:61831433-61831452 Msa1300290:CDS 35.0%
TCTCGTAGTCTTTCTCAATT+TGG + chr8_3:61831758-61831777 Msa1300290:intron 35.0%
TTCTCAATTTGGTTGTGCAA+AGG + chr8_3:61831769-61831788 Msa1300290:three_prime_UTR 35.0%
GCTAGAAGTTTGTAAGTGAT+AGG + chr8_3:61831797-61831816 Msa1300290:three_prime_UTR 35.0%
AATTGTTAGCCGAGTTTACT+AGG + chr8_3:61831887-61831906 Msa1300290:three_prime_UTR 35.0%
TTACCACAACCTAGTAAACT+CGG - chr8_3:61831899-61831918 None:intergenic 35.0%
! TACCACTGATGATGTTCATG+AGG + chr8_3:61828882-61828901 Msa1300290:five_prime_UTR 40.0%
! ACCACTGATGATGTTCATGA+GGG + chr8_3:61828883-61828902 Msa1300290:five_prime_UTR 40.0%
GTAAATCCAGAGACAGATCT+AGG + chr8_3:61828931-61828950 Msa1300290:five_prime_UTR 40.0%
TTTATACACTCTCCTACCGT+AGG + chr8_3:61829051-61829070 Msa1300290:intron 40.0%
AATTTGGCAGCATTTACCGT+AGG - chr8_3:61829093-61829112 None:intergenic 40.0%
TTTGGTTGTGGTAGTAACAG+AGG + chr8_3:61829329-61829348 Msa1300290:intron 40.0%
AACAGAGGAAGTGAAGAAAG+AGG + chr8_3:61829344-61829363 Msa1300290:intron 40.0%
! AAGAAAGAGGCACATGCATT+AGG + chr8_3:61829357-61829376 Msa1300290:five_prime_UTR 40.0%
ATTGGGGAATCAAATTTGGC+GGG + chr8_3:61829390-61829409 Msa1300290:five_prime_UTR 40.0%
ACTTCGTTGAAGAAGAGTTG+TGG - chr8_3:61829477-61829496 None:intergenic 40.0%
AGAAGAAGAAGAAGCGAAAG+GGG - chr8_3:61829559-61829578 None:intergenic 40.0%
TGAAACCTGTGTGAGATGTT+GGG - chr8_3:61829683-61829702 None:intergenic 40.0%
ATGAAACCTGTGTGAGATGT+TGG - chr8_3:61829684-61829703 None:intergenic 40.0%
!! CATTGTTTTTCTCTTCCCGA+AGG + chr8_3:61829890-61829909 Msa1300290:intron 40.0%
ACTGAGGAAAAAACTAGCTG+AGG + chr8_3:61830415-61830434 Msa1300290:CDS 40.0%
GTAATAAATGTGCTGTCAGG+TGG + chr8_3:61830529-61830548 Msa1300290:intron 40.0%
AATAAATGTGCTGTCAGGTG+GGG + chr8_3:61830531-61830550 Msa1300290:intron 40.0%
GTAATGCCTCATTGATGTAC+TGG - chr8_3:61830619-61830638 None:intergenic 40.0%
! CTCTTGCATTGAGTTGTTTC+AGG + chr8_3:61830655-61830674 Msa1300290:CDS 40.0%
!! ATACTAGTTCTGAGTAGCAC+AGG - chr8_3:61830740-61830759 None:intergenic 40.0%
! AATCCTGGGATTCTCCATTT+TGG + chr8_3:61830790-61830809 Msa1300290:intron 40.0%
CTGACCTATGGTACCTTTAT+TGG - chr8_3:61831101-61831120 None:intergenic 40.0%
!! CAATAAAGGTACCATAGGTC+AGG + chr8_3:61831099-61831118 Msa1300290:intron 40.0%
CATCTTCATTCGAAGTTCTG+AGG - chr8_3:61831335-61831354 None:intergenic 40.0%
CTCAGAACTTCGAATGAAGA+TGG + chr8_3:61831333-61831352 Msa1300290:CDS 40.0%
ACCAACTTGATTGAAGAGAC+AGG + chr8_3:61831367-61831386 Msa1300290:CDS 40.0%
TCCTGTCTCTTCAATCAAGT+TGG - chr8_3:61831371-61831390 None:intergenic 40.0%
GAAAGACTACGAGATTCTTC+AGG - chr8_3:61831752-61831771 None:intergenic 40.0%
TTCCAGCAAGGTGATGAATC+TGG + chr8_3:61828856-61828875 Msa1300290:five_prime_UTR 45.0%
TCCAGCAAGGTGATGAATCT+GGG + chr8_3:61828857-61828876 Msa1300290:five_prime_UTR 45.0%
TCCCTCATGAACATCATCAG+TGG - chr8_3:61828887-61828906 None:intergenic 45.0%
! ACTGATGATGTTCATGAGGG+AGG + chr8_3:61828886-61828905 Msa1300290:five_prime_UTR 45.0%
! CTGATGATGTTCATGAGGGA+GGG + chr8_3:61828887-61828906 Msa1300290:five_prime_UTR 45.0%
AATCCAGAGACAGATCTAGG+TGG + chr8_3:61828934-61828953 Msa1300290:intron 45.0%
ATCCAGAGACAGATCTAGGT+GGG + chr8_3:61828935-61828954 Msa1300290:intron 45.0%
GGAATCAAATTTGGCGGGAA+TGG + chr8_3:61829395-61829414 Msa1300290:exon 45.0%
TCAAATTTGGCGGGAATGGA+AGG + chr8_3:61829399-61829418 Msa1300290:exon 45.0%
!! AAGCGAAAGGGGTTAGTTAC+TGG - chr8_3:61829548-61829567 None:intergenic 45.0%
AAGATCCCAACATCTCACAC+AGG + chr8_3:61829675-61829694 Msa1300290:CDS 45.0%
TGGATCAAAAGCTCGCTGTT+TGG + chr8_3:61829849-61829868 Msa1300290:intron 45.0%
GGATCAAAAGCTCGCTGTTT+GGG + chr8_3:61829850-61829869 Msa1300290:intron 45.0%
CTGCGCATCTAATTCCAGAT+CGG - chr8_3:61830387-61830406 None:intergenic 45.0%
ATGCGCAGCTAAAATCACTG+AGG + chr8_3:61830399-61830418 Msa1300290:CDS 45.0%
CAATGCCCAGTACATCAATG+AGG + chr8_3:61830610-61830629 Msa1300290:CDS 45.0%
GTACCAAAATGGAGAATCCC+AGG - chr8_3:61830796-61830815 None:intergenic 45.0%
! TTGAAAGGGGGCCAACATTT+TGG + chr8_3:61831070-61831089 Msa1300290:intron 45.0%
! TGAAAGGGGGCCAACATTTT+GGG + chr8_3:61831071-61831090 Msa1300290:intron 45.0%
! TATTGGTGCCCCCAAAATGT+TGG - chr8_3:61831084-61831103 None:intergenic 45.0%
!!! CATTTTGGGGGCACCAATAA+AGG + chr8_3:61831085-61831104 Msa1300290:intron 45.0%
!! GGCACCAATAAAGGTACCAT+AGG + chr8_3:61831094-61831113 Msa1300290:intron 45.0%
AGCTCTGCACTATTGCAAGT+AGG + chr8_3:61831184-61831203 Msa1300290:intron 45.0%
GCAGTTGTCATGATCTCTAC+AGG - chr8_3:61831313-61831332 None:intergenic 45.0%
TTTCATCAACCCAGTTGGAC+TGG + chr8_3:61831620-61831639 Msa1300290:three_prime_UTR 45.0%
GTGATAAGACCAGTCCAACT+GGG - chr8_3:61831632-61831651 None:intergenic 45.0%
AGTGATAAGACCAGTCCAAC+TGG - chr8_3:61831633-61831652 None:intergenic 45.0%
ACTTGTCAGGCACTTCACTT+TGG - chr8_3:61831825-61831844 None:intergenic 45.0%
! TGAAGTGCCTGACAAGTTAC+AGG + chr8_3:61831828-61831847 Msa1300290:three_prime_UTR 45.0%
! GAAGTGCCTGACAAGTTACA+GGG + chr8_3:61831829-61831848 Msa1300290:three_prime_UTR 45.0%
! GTATCTCCCTGTAACTTGTC+AGG - chr8_3:61831838-61831857 None:intergenic 45.0%
TAGCCGAGTTTACTAGGTTG+TGG + chr8_3:61831893-61831912 Msa1300290:three_prime_UTR 45.0%
!! AATTTAATTAACTTTAATTA+GGG + chr8_3:61829741-61829760 Msa1300290:intron 5.0%
ACCCAGATTCATCACCTTGC+TGG - chr8_3:61828861-61828880 None:intergenic 50.0%
ATGAGGGAGGGAAGCAAAGA+AGG + chr8_3:61828899-61828918 Msa1300290:five_prime_UTR 50.0%
AGGGAAGCAAAGAAGGAAGC+TGG + chr8_3:61828906-61828925 Msa1300290:five_prime_UTR 50.0%
AGAGGGGAATAGGTCTCCTA+CGG - chr8_3:61829070-61829089 None:intergenic 50.0%
AGACCTATTCCCCTCTCCTA+CGG + chr8_3:61829074-61829093 Msa1300290:intron 50.0%
TTACCGTAGGAGAGGGGAAT+AGG - chr8_3:61829080-61829099 None:intergenic 50.0%
CAGCATTTACCGTAGGAGAG+GGG - chr8_3:61829086-61829105 None:intergenic 50.0%
GCAGCATTTACCGTAGGAGA+GGG - chr8_3:61829087-61829106 None:intergenic 50.0%
!! GAGCGACGCTGTTTTCGAAT+CGG + chr8_3:61829437-61829456 Msa1300290:CDS 50.0%
AGCATCCACCAAGCTCAATG+CGG + chr8_3:61829642-61829661 Msa1300290:CDS 50.0%
AGTAGCTTCCGCATTGAGCT+TGG - chr8_3:61829653-61829672 None:intergenic 50.0%
!! TCCTGGTTCTACTTCCGATC+TGG + chr8_3:61830370-61830389 Msa1300290:CDS 50.0%
TCCAGATCGGAAGTAGAACC+AGG - chr8_3:61830374-61830393 None:intergenic 50.0%
! GAAAGGGGGCCAACATTTTG+GGG + chr8_3:61831072-61831091 Msa1300290:intron 50.0%
! AAAGGGGGCCAACATTTTGG+GGG + chr8_3:61831073-61831092 Msa1300290:intron 50.0%
TTGCAATAGTGCAGAGCTCC+AGG - chr8_3:61831182-61831201 None:intergenic 50.0%
GTCCCACCTAGATCTGTCTC+TGG - chr8_3:61828940-61828959 None:intergenic 55.0%
GGGAATAGGTCTCCTACGGT+AGG - chr8_3:61829066-61829085 None:intergenic 55.0%
GGCAGCATTTACCGTAGGAG+AGG - chr8_3:61829088-61829107 None:intergenic 55.0%
AGCTTCCGCATTGAGCTTGG+TGG - chr8_3:61829650-61829669 None:intergenic 55.0%
GCGGAGAATCATGGTCAACG+TGG + chr8_3:61829977-61829996 Msa1300290:intron 55.0%
GCGATCTAGGCCGCATATCT+GGG - chr8_3:61830044-61830063 None:intergenic 55.0%
CAGATATGCGGCCTAGATCG+CGG + chr8_3:61830043-61830062 Msa1300290:intron 55.0%
AAGGTATGTGGCCGCGATCT+AGG - chr8_3:61830057-61830076 None:intergenic 55.0%
CGCGATCTAGGCCGCATATC+TGG - chr8_3:61830045-61830064 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 61828822 61832026 61828822 ID=Msa1300290;Name=Msa1300290
chr8_3 mRNA 61828822 61832026 61828822 ID=Msa1300290-mRNA-1;Parent=Msa1300290;Name=Msa1300290-mRNA-1;_AED=0.13;_eAED=0.13;_QI=196|0.87|0.88|1|0.62|0.55|9|375|199
chr8_3 exon 61828822 61828952 61828822 ID=Msa1300290-mRNA-1:exon:18282;Parent=Msa1300290-mRNA-1
chr8_3 exon 61829349 61829547 61829349 ID=Msa1300290-mRNA-1:exon:18283;Parent=Msa1300290-mRNA-1
chr8_3 exon 61829639 61829696 61829639 ID=Msa1300290-mRNA-1:exon:18284;Parent=Msa1300290-mRNA-1
chr8_3 exon 61830359 61830436 61830359 ID=Msa1300290-mRNA-1:exon:18285;Parent=Msa1300290-mRNA-1
chr8_3 exon 61830548 61830677 61830548 ID=Msa1300290-mRNA-1:exon:18286;Parent=Msa1300290-mRNA-1
chr8_3 exon 61831317 61831454 61831317 ID=Msa1300290-mRNA-1:exon:18287;Parent=Msa1300290-mRNA-1
chr8_3 exon 61831545 61831743 61831545 ID=Msa1300290-mRNA-1:exon:18288;Parent=Msa1300290-mRNA-1
chr8_3 exon 61831766 61831932 61831766 ID=Msa1300290-mRNA-1:exon:18289;Parent=Msa1300290-mRNA-1
chr8_3 exon 61831956 61832026 61831956 ID=Msa1300290-mRNA-1:exon:18290;Parent=Msa1300290-mRNA-1
chr8_3 five_prime_UTR 61828822 61828952 61828822 ID=Msa1300290-mRNA-1:five_prime_utr;Parent=Msa1300290-mRNA-1
chr8_3 five_prime_UTR 61829349 61829413 61829349 ID=Msa1300290-mRNA-1:five_prime_utr;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61829414 61829547 61829414 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61829639 61829696 61829639 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61830359 61830436 61830359 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61830548 61830677 61830548 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61831317 61831454 61831317 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 CDS 61831545 61831606 61831545 ID=Msa1300290-mRNA-1:cds;Parent=Msa1300290-mRNA-1
chr8_3 three_prime_UTR 61831607 61831743 61831607 ID=Msa1300290-mRNA-1:three_prime_utr;Parent=Msa1300290-mRNA-1
chr8_3 three_prime_UTR 61831766 61831932 61831766 ID=Msa1300290-mRNA-1:three_prime_utr;Parent=Msa1300290-mRNA-1
chr8_3 three_prime_UTR 61831956 61832026 61831956 ID=Msa1300290-mRNA-1:three_prime_utr;Parent=Msa1300290-mRNA-1
Gene Sequence

>Msa1300290

ATGGAAGGAAGCATGAGCAGAGAGAGCGACGCTGTTTTCGAATCGGTGATGAATCTGAATCCACAACTCTTCTTCAACGAAGTTCTCAACACTGTTGATGATTTCGTCCTCGATACTTTCGATTTCTACTTCCAGGAAGCATCCACCAAGCTCAATGCGGAAGCTACTCAAAGATCCCAACATCTCACACAGAATGATGTTGATCCTGGTTCTACTTCCGATCTGGAATTAGATGCGCAGCTAAAATCACTGAGGAAAAAACTAGCTGAGGTGGGGAAAGAGTCTGAAACGCTGAATCAAGAAATTCATGCATTAGAGAGGCAGTCTTCTCACAATGCCCAGTACATCAATGAGGCATTACAATTATTTGAGCAAAACTCTTGCATTGAGTTGTTTCAGGAGATCATGACAACTGCCTCAGAACTTCGAATGAAGATGGGAAAGCTTAACACCAACTTGATTGAAGAGACAGGAAAAGTGAAAGCAAAAAGGATTGACAACAATAAAATGGACATTTCTGCTATATATGCTTCCAAAGGCGTATCTAATACGAAATTCGAAGATCTTCAAGAATTTGTAACTCTTATGAAGAGCACGTGA
Protein sequence

>Msa1300290

MEGSMSRESDAVFESVMNLNPQLFFNEVLNTVDDFVLDTFDFYFQEASTKLNAEATQRSQHLTQNDVDPGSTSDLELDAQLKSLRKKLAEVGKESETLNQEIHALERQSSHNAQYINEALQLFEQNSCIELFQEIMTTASELRMKMGKLNTNLIEETGKVKAKRIDNNKMDISAIYASKGVSNTKFEDLQEFVTLMKST*