AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1262940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262940 MtrunA17_Chr4g0065741 90.303 165 12 3 1 164 1 162 8.97e-94 269
Msa1262940 MtrunA17_Chr3g0088511 39.130 92 56 0 72 163 31 122 8.36e-19 77.8
Msa1262940 MtrunA17_Chr1g0198431 44.565 92 50 1 59 150 179 269 2.70e-16 73.9
Msa1262940 MtrunA17_Chr6g0481071 38.272 81 50 0 73 153 95 175 8.74e-16 70.9
Msa1262940 MtrunA17_Chr4g0061451 39.286 84 50 1 67 150 37 119 1.74e-15 71.6
Msa1262940 MtrunA17_Chr4g0031091 33.962 106 61 1 47 152 16 112 3.23e-15 67.8
Msa1262940 MtrunA17_Chr4g0035031 41.558 77 45 0 74 150 9 85 3.39e-15 69.7
Msa1262940 MtrunA17_Chr7g0263001 38.462 104 61 2 49 151 12 113 4.20e-15 68.2
Msa1262940 MtrunA17_Chr7g0219021 40.698 86 47 1 71 152 38 123 5.26e-15 70.1
Msa1262940 MtrunA17_Chr7g0266751 34.524 84 55 0 74 157 33 116 7.14e-15 67.8
Msa1262940 MtrunA17_Chr3g0119311 38.961 77 47 0 73 149 36 112 7.80e-15 67.4
Msa1262940 MtrunA17_Chr3g0121631 38.272 81 49 1 71 151 34 113 6.00e-14 65.5
Msa1262940 MtrunA17_Chr7g0259641 40.230 87 52 0 74 160 274 360 1.21e-13 67.8
Msa1262940 MtrunA17_Chr4g0035051 37.662 77 48 0 74 150 9 85 1.70e-13 63.5
Msa1262940 MtrunA17_Chr4g0035001 37.662 77 48 0 74 150 7 83 5.97e-13 63.2
Msa1262940 MtrunA17_Chr7g0259671 40.230 87 52 0 74 160 280 366 6.76e-13 65.5
Msa1262940 MtrunA17_Chr3g0086861 34.146 82 53 1 69 150 41 121 7.58e-13 65.1
Msa1262940 MtrunA17_Chr4g0050781 37.179 78 49 0 74 151 8 85 1.05e-12 63.2
Msa1262940 MtrunA17_Chr2g0327921 36.585 82 52 0 73 154 7 88 1.54e-12 60.1
Msa1262940 MtrunA17_Chr2g0278661 37.179 78 49 0 74 151 8 85 5.02e-12 62.0
Msa1262940 MtrunA17_Chr4g0042151 35.065 77 50 0 75 151 46 122 5.57e-12 60.8
Msa1262940 MtrunA17_Chr5g0430471 36.782 87 55 0 64 150 30 116 9.76e-12 59.7
Msa1262940 MtrunA17_Chr7g0260761 42.857 77 44 0 74 150 278 354 6.01e-11 59.7
Msa1262940 MtrunA17_Chr3g0080801 37.931 87 54 0 63 149 58 144 9.53e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1262940 AT5G19030.1 53.012 83 39 0 76 158 80 162 1.88e-25 96.3
Msa1262940 AT4G20030.1 43.529 85 48 0 68 152 35 119 6.91e-20 81.3
Msa1262940 AT5G19030.5 53.846 65 30 0 76 140 80 144 4.99e-19 79.0
Msa1262940 AT5G19030.2 54.839 62 28 0 76 137 80 141 8.06e-18 75.9
Msa1262940 AT3G23830.2 43.373 83 47 0 72 154 34 116 1.22e-17 75.1
Msa1262940 AT3G23830.1 43.373 83 47 0 72 154 34 116 1.22e-17 75.1
Msa1262940 AT4G13850.3 41.772 79 46 0 72 150 34 112 4.29e-17 73.9
Msa1262940 AT4G13850.4 41.250 80 47 0 72 151 34 113 4.41e-17 73.6
Msa1262940 AT4G13850.2 41.772 79 46 0 72 150 34 112 5.27e-17 73.9
Msa1262940 AT4G13850.1 41.772 79 46 0 72 150 34 112 5.89e-17 73.9
Msa1262940 AT1G74230.1 40.860 93 52 2 57 149 20 109 3.50e-15 71.6
Msa1262940 AT5G61030.2 31.496 127 71 3 25 151 8 118 1.12e-14 70.1
Msa1262940 AT5G61030.1 31.496 127 71 3 25 151 8 118 1.12e-14 70.1
Msa1262940 AT4G39260.3 37.662 77 48 0 74 150 7 83 1.21e-14 66.2
Msa1262940 AT3G08000.1 40.187 107 55 2 43 149 20 117 1.45e-14 67.4
Msa1262940 AT3G20930.1 45.455 77 42 0 74 150 283 359 4.01e-14 68.9
Msa1262940 AT4G39260.2 37.662 77 48 0 74 150 7 83 4.59e-14 65.5
Msa1262940 AT4G39260.1 37.662 77 48 0 74 150 7 83 1.13e-13 65.5
Msa1262940 AT5G06210.1 32.353 102 69 0 49 150 10 111 2.18e-13 64.3
Msa1262940 AT1G73530.1 34.783 92 60 0 73 164 77 168 8.35e-13 63.5
Msa1262940 AT5G47320.1 41.772 79 46 0 72 150 30 108 8.52e-13 63.9
Msa1262940 AT3G46020.1 35.443 79 51 0 72 150 6 84 1.02e-12 61.2
Msa1262940 AT2G37510.1 33.333 81 54 0 66 146 27 107 1.17e-12 62.4
Msa1262940 AT3G20930.2 42.683 82 42 1 74 150 283 364 6.19e-12 62.8
Msa1262940 AT4G19610.1 36.364 77 49 0 74 150 295 371 7.49e-12 62.8
Msa1262940 AT5G08695.1 37.179 78 49 0 74 151 228 305 7.59e-12 62.8
Msa1262940 AT2G27330.1 35.443 79 51 0 72 150 20 98 8.01e-12 59.3
Msa1262940 AT5G54580.1 32.710 107 61 2 56 151 28 134 8.39e-12 60.1
Msa1262940 AT3G10400.1 34.066 91 58 1 75 163 59 149 1.85e-11 60.8
Msa1262940 AT3G47120.2 35.227 88 57 0 60 147 23 110 4.45e-11 60.1
Msa1262940 AT5G09880.1 35.165 91 58 1 58 147 250 340 4.58e-11 60.5
Msa1262940 AT3G47120.1 35.227 88 57 0 60 147 23 110 8.21e-11 59.7

Find 39 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACCTTTGATGAAGATTT+TGG 0.183699 8_2:+65935454 None:intergenic
CTCTAGGATTTGCATATATT+TGG 0.190867 8_2:-65934365 Msa1262940:CDS
GATGAAGGAATACTACAATT+TGG 0.244629 8_2:+65935633 None:intergenic
ATGAAGGAATACTACAATTT+GGG 0.280015 8_2:+65935634 None:intergenic
TTCCTTCATCTGTCTGGGTC+TGG 0.287764 8_2:-65935620 Msa1262940:CDS
AATTTCTAGCATTGTGCCTT+GGG 0.295341 8_2:-65934054 Msa1262940:three_prime_UTR
GTAGTATTCCTTCATCTGTC+TGG 0.300936 8_2:-65935626 Msa1262940:CDS
CTATATTGAACAGTGTATTA+AGG 0.347802 8_2:-65933887 Msa1262940:three_prime_UTR
TCCTTCATCTGTCTGGGTCT+GGG 0.350180 8_2:-65935619 Msa1262940:CDS
TCATGTCAGTTAAAATTTGT+AGG 0.382544 8_2:+65934139 None:intergenic
AATCTAATGGCACTTGTTGT+TGG 0.399086 8_2:-65935677 Msa1262940:exon
GAATTTCTAGCATTGTGCCT+TGG 0.403850 8_2:-65934055 Msa1262940:three_prime_UTR
TATGTTACAATTGCAAAGCC+TGG 0.436181 8_2:-65934188 Msa1262940:CDS
CTTAATACACTGTTCAATAT+AGG 0.451311 8_2:+65933888 None:intergenic
TAGTATTCCTTCATCTGTCT+GGG 0.455311 8_2:-65935625 Msa1262940:CDS
GTGTTGTGTTCAAAATCTAA+TGG 0.463347 8_2:-65935690 Msa1262940:exon
CACCTGTCACCGTCAGTGAT+TGG 0.480273 8_2:+65933948 None:intergenic
GACAGAAGATTCAGAGGAGG+TGG 0.482508 8_2:+65935499 None:intergenic
TGCCAATCACTGACGGTGAC+AGG 0.491174 8_2:-65933950 Msa1262940:three_prime_UTR
TCCCAGACCCAGACAGATGA+AGG 0.512288 8_2:+65935618 None:intergenic
TCAGCTGCAAAAGAGATGAA+TGG 0.519432 8_2:-65934318 Msa1262940:CDS
TCAACCAAAATCTTCATCAA+AGG 0.523578 8_2:-65935458 Msa1262940:intron
GAAATTGAAAGTGAAAGAGT+TGG 0.531902 8_2:+65935560 None:intergenic
CTGTTTGGTGAAGTTACTCA+AGG 0.549668 8_2:-65934557 Msa1262940:intron
ATTTCTAGCATTGTGCCTTG+GGG 0.561081 8_2:-65934053 Msa1262940:three_prime_UTR
TGCATATATTTGGTTTGTCG+AGG 0.565736 8_2:-65934355 Msa1262940:CDS
GAGGAGGTGGAGCAAAAGCA+TGG 0.569253 8_2:+65935512 None:intergenic
TTTCTAGCATTGTGCCTTGG+GGG 0.582549 8_2:-65934052 Msa1262940:three_prime_UTR
TTTCGTTAAATGCACTTCAG+AGG 0.589643 8_2:+65934589 None:intergenic
TGAAGGAATACTACAATTTG+GGG 0.591870 8_2:+65935635 None:intergenic
GTGGAGCAAAAGCATGGAGA+AGG 0.598651 8_2:+65935518 None:intergenic
TGGAGAAGGTGCATAGCGAG+TGG 0.615709 8_2:+65935532 None:intergenic
CTAAAAGTGCCAATCACTGA+CGG 0.620131 8_2:-65933957 Msa1262940:three_prime_UTR
TGCACTTCAGAGGTTGAGAG+AGG 0.626279 8_2:+65934599 None:intergenic
ACATTCGACAGAAGATTCAG+AGG 0.629820 8_2:+65935493 None:intergenic
TGCAAAAGAGATGAATGGAA+AGG 0.634043 8_2:-65934313 Msa1262940:intron
AGAAGGTGCATAGCGAGTGG+AGG 0.655325 8_2:+65935535 None:intergenic
GTATTGTATGCTTACTAGAG+TGG 0.660770 8_2:+65934100 None:intergenic
TTCGACAGAAGATTCAGAGG+AGG 0.703143 8_2:+65935496 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGTAATTATAAATATATTT+TGG - chr8_2:65935079-65935098 Msa1262940:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG - chr8_2:65935080-65935099 Msa1262940:intron 10.0%
!!! AGGTAATTATAAATATATTT+TGG - chr8_2:65935079-65935098 Msa1262940:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG - chr8_2:65935080-65935099 Msa1262940:intron 10.0%
!!! ATCAAATTGAATTAGAATTT+TGG - chr8_2:65934383-65934402 Msa1262940:CDS 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG - chr8_2:65934384-65934403 Msa1262940:CDS 15.0%
!! ATACAACAAGATAAAATAAA+TGG - chr8_2:65934470-65934489 Msa1262940:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG - chr8_2:65934801-65934820 Msa1262940:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG - chr8_2:65935192-65935211 Msa1262940:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG - chr8_2:65935224-65935243 Msa1262940:intron 15.0%
!!! ATCAAATTGAATTAGAATTT+TGG - chr8_2:65934383-65934402 Msa1262940:CDS 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG - chr8_2:65934384-65934403 Msa1262940:CDS 15.0%
!! ATACAACAAGATAAAATAAA+TGG - chr8_2:65934470-65934489 Msa1262940:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG - chr8_2:65934801-65934820 Msa1262940:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG - chr8_2:65935192-65935211 Msa1262940:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG - chr8_2:65935224-65935243 Msa1262940:intron 15.0%
!!! TTTTCATATAATTAGATCGT+AGG + chr8_2:65934794-65934813 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG - chr8_2:65935128-65935147 Msa1262940:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG - chr8_2:65935223-65935242 Msa1262940:intron 20.0%
!!! TTTTCATATAATTAGATCGT+AGG + chr8_2:65934794-65934813 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG - chr8_2:65935128-65935147 Msa1262940:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG - chr8_2:65935223-65935242 Msa1262940:intron 20.0%
! ATGAAGGAATACTACAATTT+GGG + chr8_2:65933985-65934004 None:intergenic 25.0%
! AGATTAATTTCTTGGAACTT+CGG - chr8_2:65934508-65934527 Msa1262940:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG + chr8_2:65934540-65934559 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG + chr8_2:65934632-65934651 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG + chr8_2:65934633-65934652 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG + chr8_2:65934734-65934753 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG - chr8_2:65934946-65934965 Msa1262940:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG - chr8_2:65935143-65935162 Msa1262940:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG + chr8_2:65935480-65935499 None:intergenic 25.0%
! CTTAATACACTGTTCAATAT+AGG + chr8_2:65935731-65935750 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG - chr8_2:65935729-65935748 Msa1262940:five_prime_UTR 25.0%
! ATGAAGGAATACTACAATTT+GGG + chr8_2:65933985-65934004 None:intergenic 25.0%
! AGATTAATTTCTTGGAACTT+CGG - chr8_2:65934508-65934527 Msa1262940:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG + chr8_2:65934540-65934559 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG + chr8_2:65934632-65934651 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG + chr8_2:65934633-65934652 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG + chr8_2:65934734-65934753 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG - chr8_2:65934946-65934965 Msa1262940:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG - chr8_2:65935143-65935162 Msa1262940:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG + chr8_2:65935480-65935499 None:intergenic 25.0%
! CTTAATACACTGTTCAATAT+AGG + chr8_2:65935731-65935750 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG - chr8_2:65935729-65935748 Msa1262940:five_prime_UTR 25.0%
GTGTTGTGTTCAAAATCTAA+TGG - chr8_2:65933926-65933945 Msa1262940:three_prime_UTR 30.0%
TGAAGGAATACTACAATTTG+GGG + chr8_2:65933984-65934003 None:intergenic 30.0%
GATGAAGGAATACTACAATT+TGG + chr8_2:65933986-65934005 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG + chr8_2:65934059-65934078 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG + chr8_2:65934155-65934174 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG - chr8_2:65934158-65934177 Msa1262940:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG + chr8_2:65934165-65934184 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG + chr8_2:65934198-65934217 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG - chr8_2:65934196-65934215 Msa1262940:CDS 30.0%
CTATCCCTTTAATCAATAAC+GGG - chr8_2:65934197-65934216 Msa1262940:CDS 30.0%
! GAAACCCGTTATTGATTAAA+GGG + chr8_2:65934204-65934223 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG + chr8_2:65934205-65934224 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG - chr8_2:65934332-65934351 Msa1262940:CDS 30.0%
GGGACAATGATAACAAATTA+AGG - chr8_2:65934404-65934423 Msa1262940:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG + chr8_2:65934443-65934462 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG + chr8_2:65934505-65934524 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG + chr8_2:65934509-65934528 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG + chr8_2:65934631-65934650 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG - chr8_2:65934698-65934717 Msa1262940:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG - chr8_2:65934741-65934760 Msa1262940:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG - chr8_2:65934742-65934761 Msa1262940:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG - chr8_2:65934745-65934764 Msa1262940:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG + chr8_2:65934785-65934804 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG + chr8_2:65934786-65934805 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG + chr8_2:65934859-65934878 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG - chr8_2:65934873-65934892 Msa1262940:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG - chr8_2:65935094-65935113 Msa1262940:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG - chr8_2:65935162-65935181 Msa1262940:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG - chr8_2:65935251-65935270 Msa1262940:intron 30.0%
GTCATCAACATTGTATAGTA+TGG + chr8_2:65935362-65935381 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG - chr8_2:65935395-65935414 Msa1262940:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG + chr8_2:65935449-65935468 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG + chr8_2:65935653-65935672 None:intergenic 30.0%
GTGTTGTGTTCAAAATCTAA+TGG - chr8_2:65933926-65933945 Msa1262940:three_prime_UTR 30.0%
TGAAGGAATACTACAATTTG+GGG + chr8_2:65933984-65934003 None:intergenic 30.0%
GATGAAGGAATACTACAATT+TGG + chr8_2:65933986-65934005 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG + chr8_2:65934059-65934078 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG + chr8_2:65934155-65934174 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG - chr8_2:65934158-65934177 Msa1262940:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG + chr8_2:65934165-65934184 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG + chr8_2:65934198-65934217 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG - chr8_2:65934196-65934215 Msa1262940:CDS 30.0%
CTATCCCTTTAATCAATAAC+GGG - chr8_2:65934197-65934216 Msa1262940:CDS 30.0%
! GAAACCCGTTATTGATTAAA+GGG + chr8_2:65934204-65934223 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG + chr8_2:65934205-65934224 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG - chr8_2:65934332-65934351 Msa1262940:CDS 30.0%
GGGACAATGATAACAAATTA+AGG - chr8_2:65934404-65934423 Msa1262940:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG + chr8_2:65934443-65934462 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG + chr8_2:65934505-65934524 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG + chr8_2:65934509-65934528 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG + chr8_2:65934631-65934650 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG - chr8_2:65934698-65934717 Msa1262940:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG - chr8_2:65934741-65934760 Msa1262940:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG - chr8_2:65934742-65934761 Msa1262940:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG - chr8_2:65934745-65934764 Msa1262940:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG + chr8_2:65934785-65934804 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG + chr8_2:65934786-65934805 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG + chr8_2:65934859-65934878 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG - chr8_2:65934873-65934892 Msa1262940:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG - chr8_2:65935094-65935113 Msa1262940:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG - chr8_2:65935162-65935181 Msa1262940:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG - chr8_2:65935251-65935270 Msa1262940:intron 30.0%
GTCATCAACATTGTATAGTA+TGG + chr8_2:65935362-65935381 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG - chr8_2:65935395-65935414 Msa1262940:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG + chr8_2:65935449-65935468 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG + chr8_2:65935653-65935672 None:intergenic 30.0%
!! AATCTAATGGCACTTGTTGT+TGG - chr8_2:65933939-65933958 Msa1262940:three_prime_UTR 35.0%
TAGTATTCCTTCATCTGTCT+GGG - chr8_2:65933991-65934010 Msa1262940:three_prime_UTR 35.0%
! CCGTTATTGATTAAAGGGAT+AGG + chr8_2:65934199-65934218 None:intergenic 35.0%
! ACGGGTTTCTTTCAATTTTG+TGG - chr8_2:65934215-65934234 Msa1262940:intron 35.0%
ATGAGAATAGAAGGCTCTTA+CGG - chr8_2:65934341-65934360 Msa1262940:CDS 35.0%
TTCCGACCAGATTAATTTCT+TGG - chr8_2:65934500-65934519 Msa1262940:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG + chr8_2:65934931-65934950 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG - chr8_2:65934976-65934995 Msa1262940:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG + chr8_2:65935030-65935049 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+TGG - chr8_2:65935044-65935063 Msa1262940:intron 35.0%
!! TGCATATATTTGGTTTGTCG+AGG - chr8_2:65935261-65935280 Msa1262940:intron 35.0%
TGCAAAAGAGATGAATGGAA+AGG - chr8_2:65935303-65935322 Msa1262940:intron 35.0%
TATGTTACAATTGCAAAGCC+TGG - chr8_2:65935428-65935447 Msa1262940:intron 35.0%
GTATTGTATGCTTACTAGAG+TGG + chr8_2:65935519-65935538 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG - chr8_2:65935562-65935581 Msa1262940:CDS 35.0%
!! AATCTAATGGCACTTGTTGT+TGG - chr8_2:65933939-65933958 Msa1262940:three_prime_UTR 35.0%
TAGTATTCCTTCATCTGTCT+GGG - chr8_2:65933991-65934010 Msa1262940:three_prime_UTR 35.0%
! CCGTTATTGATTAAAGGGAT+AGG + chr8_2:65934199-65934218 None:intergenic 35.0%
! ACGGGTTTCTTTCAATTTTG+TGG - chr8_2:65934215-65934234 Msa1262940:intron 35.0%
ATGAGAATAGAAGGCTCTTA+CGG - chr8_2:65934341-65934360 Msa1262940:CDS 35.0%
TTCCGACCAGATTAATTTCT+TGG - chr8_2:65934500-65934519 Msa1262940:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG + chr8_2:65934931-65934950 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG - chr8_2:65934976-65934995 Msa1262940:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG + chr8_2:65935030-65935049 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+TGG - chr8_2:65935044-65935063 Msa1262940:intron 35.0%
!! TGCATATATTTGGTTTGTCG+AGG - chr8_2:65935261-65935280 Msa1262940:intron 35.0%
TGCAAAAGAGATGAATGGAA+AGG - chr8_2:65935303-65935322 Msa1262940:intron 35.0%
TATGTTACAATTGCAAAGCC+TGG - chr8_2:65935428-65935447 Msa1262940:intron 35.0%
GTATTGTATGCTTACTAGAG+TGG + chr8_2:65935519-65935538 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG - chr8_2:65935562-65935581 Msa1262940:CDS 35.0%
GTAGTATTCCTTCATCTGTC+TGG - chr8_2:65933990-65934009 Msa1262940:three_prime_UTR 40.0%
ACATTCGACAGAAGATTCAG+AGG + chr8_2:65934126-65934145 None:intergenic 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG + chr8_2:65934627-65934646 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG - chr8_2:65934655-65934674 Msa1262940:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG + chr8_2:65934828-65934847 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG + chr8_2:65934829-65934848 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG + chr8_2:65934958-65934977 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG + chr8_2:65934964-65934983 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG - chr8_2:65934980-65934999 Msa1262940:intron 40.0%
! CTGTTTGGTGAAGTTACTCA+AGG - chr8_2:65935059-65935078 Msa1262940:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG - chr8_2:65935235-65935254 Msa1262940:intron 40.0%
TCAGCTGCAAAAGAGATGAA+TGG - chr8_2:65935298-65935317 Msa1262940:intron 40.0%
!! AATGTTGATGACATGTGAGC+TGG - chr8_2:65935370-65935389 Msa1262940:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG - chr8_2:65935399-65935418 Msa1262940:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG - chr8_2:65935561-65935580 Msa1262940:CDS 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG - chr8_2:65935563-65935582 Msa1262940:CDS 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG - chr8_2:65935659-65935678 Msa1262940:CDS 40.0%
AATGTGCAAGTGCAACATTG+GGG + chr8_2:65933880-65933899 None:intergenic 40.0%
GAATGTGCAAGTGCAACATT+GGG + chr8_2:65933881-65933900 None:intergenic 40.0%
AGAATGTGCAAGTGCAACAT+TGG + chr8_2:65933882-65933901 None:intergenic 40.0%
GTAGTATTCCTTCATCTGTC+TGG - chr8_2:65933990-65934009 Msa1262940:three_prime_UTR 40.0%
ACATTCGACAGAAGATTCAG+AGG + chr8_2:65934126-65934145 None:intergenic 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG + chr8_2:65934627-65934646 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG - chr8_2:65934655-65934674 Msa1262940:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG + chr8_2:65934828-65934847 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG + chr8_2:65934829-65934848 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG + chr8_2:65934958-65934977 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG + chr8_2:65934964-65934983 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG - chr8_2:65934980-65934999 Msa1262940:intron 40.0%
! CTGTTTGGTGAAGTTACTCA+AGG - chr8_2:65935059-65935078 Msa1262940:intron 40.0%
AAAAAAACTGGGCAGTCTCT+AGG - chr8_2:65935235-65935254 Msa1262940:intron 40.0%
TCAGCTGCAAAAGAGATGAA+TGG - chr8_2:65935298-65935317 Msa1262940:intron 40.0%
!! AATGTTGATGACATGTGAGC+TGG - chr8_2:65935370-65935389 Msa1262940:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG - chr8_2:65935399-65935418 Msa1262940:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG - chr8_2:65935561-65935580 Msa1262940:CDS 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG - chr8_2:65935563-65935582 Msa1262940:CDS 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG - chr8_2:65935659-65935678 Msa1262940:CDS 40.0%
TTCGACAGAAGATTCAGAGG+AGG + chr8_2:65934123-65934142 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG + chr8_2:65934595-65934614 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG + chr8_2:65934596-65934615 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG + chr8_2:65934717-65934736 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG + chr8_2:65934837-65934856 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG + chr8_2:65934921-65934940 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG + chr8_2:65934927-65934946 None:intergenic 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG - chr8_2:65935379-65935398 Msa1262940:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG - chr8_2:65935564-65935583 Msa1262940:CDS 45.0%
TTTACCAAAACGAACCCCCA+AGG + chr8_2:65935581-65935600 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG + chr8_2:65934123-65934142 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG + chr8_2:65934595-65934614 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG + chr8_2:65934596-65934615 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG + chr8_2:65934717-65934736 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG + chr8_2:65934837-65934856 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG + chr8_2:65934921-65934940 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG + chr8_2:65934927-65934946 None:intergenic 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG - chr8_2:65935379-65935398 Msa1262940:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG - chr8_2:65935564-65935583 Msa1262940:CDS 45.0%
TTTACCAAAACGAACCCCCA+AGG + chr8_2:65935581-65935600 None:intergenic 45.0%
TTCCTTCATCTGTCTGGGTC+TGG - chr8_2:65933996-65934015 Msa1262940:three_prime_UTR 50.0%
TCCTTCATCTGTCTGGGTCT+GGG - chr8_2:65933997-65934016 Msa1262940:three_prime_UTR 50.0%
! GTGGAGCAAAAGCATGGAGA+AGG + chr8_2:65934101-65934120 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG + chr8_2:65934120-65934139 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG - chr8_2:65934594-65934613 Msa1262940:CDS 50.0%
! CTTGTCCAGAAGTGCACATC+CGG + chr8_2:65934677-65934696 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG + chr8_2:65935020-65935039 None:intergenic 50.0%
TTCCTTCATCTGTCTGGGTC+TGG - chr8_2:65933996-65934015 Msa1262940:three_prime_UTR 50.0%
TCCTTCATCTGTCTGGGTCT+GGG - chr8_2:65933997-65934016 Msa1262940:three_prime_UTR 50.0%
! GTGGAGCAAAAGCATGGAGA+AGG + chr8_2:65934101-65934120 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG + chr8_2:65934120-65934139 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG - chr8_2:65934594-65934613 Msa1262940:CDS 50.0%
! CTTGTCCAGAAGTGCACATC+CGG + chr8_2:65934677-65934696 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG + chr8_2:65935020-65935039 None:intergenic 50.0%
TCCCAGACCCAGACAGATGA+AGG + chr8_2:65934001-65934020 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG + chr8_2:65934084-65934103 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG + chr8_2:65934087-65934106 None:intergenic 55.0%
GAGGAGGTGGAGCAAAAGCA+TGG + chr8_2:65934107-65934126 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG - chr8_2:65934426-65934445 Msa1262940:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG + chr8_2:65934590-65934609 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG + chr8_2:65934591-65934610 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG - chr8_2:65934990-65935009 Msa1262940:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG - chr8_2:65934991-65935010 Msa1262940:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG + chr8_2:65935006-65935025 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG - chr8_2:65935574-65935593 Msa1262940:CDS 55.0%
TGCCAATCACTGACGGTGAC+AGG - chr8_2:65935666-65935685 Msa1262940:CDS 55.0%
CACCTGTCACCGTCAGTGAT+TGG + chr8_2:65935671-65935690 None:intergenic 55.0%
TCCCAGACCCAGACAGATGA+AGG + chr8_2:65934001-65934020 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG + chr8_2:65934084-65934103 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG + chr8_2:65934087-65934106 None:intergenic 55.0%
GAGGAGGTGGAGCAAAAGCA+TGG + chr8_2:65934107-65934126 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG - chr8_2:65934426-65934445 Msa1262940:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG + chr8_2:65934590-65934609 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG + chr8_2:65934591-65934610 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG - chr8_2:65934990-65935009 Msa1262940:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG - chr8_2:65934991-65935010 Msa1262940:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG + chr8_2:65935006-65935025 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG - chr8_2:65935574-65935593 Msa1262940:CDS 55.0%
TGCCAATCACTGACGGTGAC+AGG - chr8_2:65935666-65935685 Msa1262940:CDS 55.0%
CACCTGTCACCGTCAGTGAT+TGG + chr8_2:65935671-65935690 None:intergenic 55.0%
AATGCATGCGGGCAGGGGTT+AGG + chr8_2:65934584-65934603 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG + chr8_2:65934589-65934608 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG - chr8_2:65934669-65934688 Msa1262940:intron 60.0%
AATGCATGCGGGCAGGGGTT+AGG + chr8_2:65934584-65934603 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG + chr8_2:65934589-65934608 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG - chr8_2:65934669-65934688 Msa1262940:intron 60.0%
CCCCTGCCCGCATGCATTGT+TGG - chr8_2:65934586-65934605 Msa1262940:CDS 65.0%
CCCCTGCCCGCATGCATTGT+TGG - chr8_2:65934586-65934605 Msa1262940:CDS 65.0%
GCATGCGGGCAGGGGTTAGG+AGG + chr8_2:65934581-65934600 None:intergenic 70.0%
GCATGCGGGCAGGGGTTAGG+AGG + chr8_2:65934581-65934600 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 65933880 65935758 65933880 ID=Msa1262940;Name=Msa1262940
chr8_2 mRNA 65933880 65935758 65933880 ID=Msa1262940-mRNA-1;Parent=Msa1262940;Name=Msa1262940-mRNA-1;_AED=0.03;_eAED=0.03;_QI=65|1|1|1|1|1|4|268|164
chr8_2 exon 65933880 65934231 65933880 ID=Msa1262940-mRNA-1:exon:21479;Parent=Msa1262940-mRNA-1
chr8_2 exon 65934314 65934420 65934314 ID=Msa1262940-mRNA-1:exon:21478;Parent=Msa1262940-mRNA-1
chr8_2 exon 65934558 65934626 65934558 ID=Msa1262940-mRNA-1:exon:21477;Parent=Msa1262940-mRNA-1
chr8_2 exon 65935459 65935758 65935459 ID=Msa1262940-mRNA-1:exon:21476;Parent=Msa1262940-mRNA-1
chr8_2 five_prime_UTR 65935694 65935758 65935694 ID=Msa1262940-mRNA-1:five_prime_utr;Parent=Msa1262940-mRNA-1
chr8_2 CDS 65935459 65935693 65935459 ID=Msa1262940-mRNA-1:cds;Parent=Msa1262940-mRNA-1
chr8_2 CDS 65934558 65934626 65934558 ID=Msa1262940-mRNA-1:cds;Parent=Msa1262940-mRNA-1
chr8_2 CDS 65934314 65934420 65934314 ID=Msa1262940-mRNA-1:cds;Parent=Msa1262940-mRNA-1
chr8_2 CDS 65934148 65934231 65934148 ID=Msa1262940-mRNA-1:cds;Parent=Msa1262940-mRNA-1
chr8_2 three_prime_UTR 65933880 65934147 65933880 ID=Msa1262940-mRNA-1:three_prime_utr;Parent=Msa1262940-mRNA-1
Gene Sequence

>Msa1262940

ATGGCACTTGTTGTTGGTAGTAGTACTATATGCAAACCCCAAATTGTAGTATTCCTTCATCTGTCTGGGTCTGGGAGTTTGAAGAAACAAACAGTTTCGAAAATCACATTTCCAACTCTTTCACTTTCAATTTCTACCTCCACTCGCTATGCACCTTCTCCATGCTTTTGCTCCACCTCCTCTGAATCTTCTGTCGAATGTAATACGCCTTCTTCAACCAAAATCTTCATCAAAGGGCTTCCTCTCTCAACCTCTGAAGTGCATTTAACGAAAGTGTTTTCACTGTTTGGTGAAGTTACTCAAGTCAAGCTTTTGATAGATAAAAAAACTGGGCAGTCTCTAGGATTTGCATATATTTGGTTTGTCGAGGAAGAGTCTGCACAATCAGCTGCAAAAGAGATGAATGGAAAGTTTTTTTATGGCAGGTTTATTTATGTTACAATTGCAAAGCCTGGATCATCAAAAAGTTTGAAGAGAAAAGCCTACAAATTTTAA
Protein sequence

>Msa1262940

MALVVGSSTICKPQIVVFLHLSGSGSLKKQTVSKITFPTLSLSISTSTRYAPSPCFCSTSSESSVECNTPSSTKIFIKGLPLSTSEVHLTKVFSLFGEVTQVKLLIDKKTGQSLGFAYIWFVEEESAQSAAKEMNGKFFYGRFIYVTIAKPGSSKSLKRKAYKF*