AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1302080

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1302080 MtrunA17_Chr4g0065741 90.303 165 12 3 1 164 1 162 8.97e-94 269
Msa1302080 MtrunA17_Chr3g0088511 39.130 92 56 0 72 163 31 122 8.36e-19 77.8
Msa1302080 MtrunA17_Chr1g0198431 44.565 92 50 1 59 150 179 269 2.70e-16 73.9
Msa1302080 MtrunA17_Chr6g0481071 38.272 81 50 0 73 153 95 175 8.74e-16 70.9
Msa1302080 MtrunA17_Chr4g0061451 39.286 84 50 1 67 150 37 119 1.74e-15 71.6
Msa1302080 MtrunA17_Chr4g0031091 33.962 106 61 1 47 152 16 112 3.23e-15 67.8
Msa1302080 MtrunA17_Chr4g0035031 41.558 77 45 0 74 150 9 85 3.39e-15 69.7
Msa1302080 MtrunA17_Chr7g0263001 38.462 104 61 2 49 151 12 113 4.20e-15 68.2
Msa1302080 MtrunA17_Chr7g0219021 40.698 86 47 1 71 152 38 123 5.26e-15 70.1
Msa1302080 MtrunA17_Chr7g0266751 34.524 84 55 0 74 157 33 116 7.14e-15 67.8
Msa1302080 MtrunA17_Chr3g0119311 38.961 77 47 0 73 149 36 112 7.80e-15 67.4
Msa1302080 MtrunA17_Chr3g0121631 38.272 81 49 1 71 151 34 113 6.00e-14 65.5
Msa1302080 MtrunA17_Chr7g0259641 40.230 87 52 0 74 160 274 360 1.21e-13 67.8
Msa1302080 MtrunA17_Chr4g0035051 37.662 77 48 0 74 150 9 85 1.70e-13 63.5
Msa1302080 MtrunA17_Chr4g0035001 37.662 77 48 0 74 150 7 83 5.97e-13 63.2
Msa1302080 MtrunA17_Chr7g0259671 40.230 87 52 0 74 160 280 366 6.76e-13 65.5
Msa1302080 MtrunA17_Chr3g0086861 34.146 82 53 1 69 150 41 121 7.58e-13 65.1
Msa1302080 MtrunA17_Chr4g0050781 37.179 78 49 0 74 151 8 85 1.05e-12 63.2
Msa1302080 MtrunA17_Chr2g0327921 36.585 82 52 0 73 154 7 88 1.54e-12 60.1
Msa1302080 MtrunA17_Chr2g0278661 37.179 78 49 0 74 151 8 85 5.02e-12 62.0
Msa1302080 MtrunA17_Chr4g0042151 35.065 77 50 0 75 151 46 122 5.57e-12 60.8
Msa1302080 MtrunA17_Chr5g0430471 36.782 87 55 0 64 150 30 116 9.76e-12 59.7
Msa1302080 MtrunA17_Chr7g0260761 42.857 77 44 0 74 150 278 354 6.01e-11 59.7
Msa1302080 MtrunA17_Chr3g0080801 37.931 87 54 0 63 149 58 144 9.53e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1302080 AT5G19030.1 53.012 83 39 0 76 158 80 162 1.88e-25 96.3
Msa1302080 AT4G20030.1 43.529 85 48 0 68 152 35 119 6.91e-20 81.3
Msa1302080 AT5G19030.5 53.846 65 30 0 76 140 80 144 4.99e-19 79.0
Msa1302080 AT5G19030.2 54.839 62 28 0 76 137 80 141 8.06e-18 75.9
Msa1302080 AT3G23830.2 43.373 83 47 0 72 154 34 116 1.22e-17 75.1
Msa1302080 AT3G23830.1 43.373 83 47 0 72 154 34 116 1.22e-17 75.1
Msa1302080 AT4G13850.3 41.772 79 46 0 72 150 34 112 4.29e-17 73.9
Msa1302080 AT4G13850.4 41.250 80 47 0 72 151 34 113 4.41e-17 73.6
Msa1302080 AT4G13850.2 41.772 79 46 0 72 150 34 112 5.27e-17 73.9
Msa1302080 AT4G13850.1 41.772 79 46 0 72 150 34 112 5.89e-17 73.9
Msa1302080 AT1G74230.1 40.860 93 52 2 57 149 20 109 3.50e-15 71.6
Msa1302080 AT5G61030.2 31.496 127 71 3 25 151 8 118 1.12e-14 70.1
Msa1302080 AT5G61030.1 31.496 127 71 3 25 151 8 118 1.12e-14 70.1
Msa1302080 AT4G39260.3 37.662 77 48 0 74 150 7 83 1.21e-14 66.2
Msa1302080 AT3G08000.1 40.187 107 55 2 43 149 20 117 1.45e-14 67.4
Msa1302080 AT3G20930.1 45.455 77 42 0 74 150 283 359 4.01e-14 68.9
Msa1302080 AT4G39260.2 37.662 77 48 0 74 150 7 83 4.59e-14 65.5
Msa1302080 AT4G39260.1 37.662 77 48 0 74 150 7 83 1.13e-13 65.5
Msa1302080 AT5G06210.1 32.353 102 69 0 49 150 10 111 2.18e-13 64.3
Msa1302080 AT1G73530.1 34.783 92 60 0 73 164 77 168 8.35e-13 63.5
Msa1302080 AT5G47320.1 41.772 79 46 0 72 150 30 108 8.52e-13 63.9
Msa1302080 AT3G46020.1 35.443 79 51 0 72 150 6 84 1.02e-12 61.2
Msa1302080 AT2G37510.1 33.333 81 54 0 66 146 27 107 1.17e-12 62.4
Msa1302080 AT3G20930.2 42.683 82 42 1 74 150 283 364 6.19e-12 62.8
Msa1302080 AT4G19610.1 36.364 77 49 0 74 150 295 371 7.49e-12 62.8
Msa1302080 AT5G08695.1 37.179 78 49 0 74 151 228 305 7.59e-12 62.8
Msa1302080 AT2G27330.1 35.443 79 51 0 72 150 20 98 8.01e-12 59.3
Msa1302080 AT5G54580.1 32.710 107 61 2 56 151 28 134 8.39e-12 60.1
Msa1302080 AT3G10400.1 34.066 91 58 1 75 163 59 149 1.85e-11 60.8
Msa1302080 AT3G47120.2 35.227 88 57 0 60 147 23 110 4.45e-11 60.1
Msa1302080 AT5G09880.1 35.165 91 58 1 58 147 250 340 4.58e-11 60.5
Msa1302080 AT3G47120.1 35.227 88 57 0 60 147 23 110 8.21e-11 59.7

Find 38 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTACCTTTGATGAAGATTT+TGG 0.183699 8_3:-64420354 None:intergenic
CTCTAGGATTTGCATATATT+TGG 0.190867 8_3:+64421446 Msa1302080:CDS
GATGAAGGAATACTACAATT+TGG 0.244629 8_3:-64420175 None:intergenic
ATGAAGGAATACTACAATTT+GGG 0.280015 8_3:-64420174 None:intergenic
TTCCTTCATCTGTCTGGGTC+TGG 0.287764 8_3:+64420188 Msa1302080:CDS
AATTTCTAGCATTGTGCCTT+GGG 0.295341 8_3:+64421757 Msa1302080:three_prime_UTR
GTAGTATTCCTTCATCTGTC+TGG 0.300936 8_3:+64420182 Msa1302080:CDS
CTATATTGAACAGTGTATTA+AGG 0.340770 8_3:+64421924 Msa1302080:three_prime_UTR
TCCTTCATCTGTCTGGGTCT+GGG 0.350180 8_3:+64420189 Msa1302080:CDS
TCATGTCAGTTAAAATTTGT+AGG 0.382544 8_3:-64421672 None:intergenic
AATCTAATGGCACTTGTTGT+TGG 0.399086 8_3:+64420131 Msa1302080:exon
GAATTTCTAGCATTGTGCCT+TGG 0.403850 8_3:+64421756 Msa1302080:three_prime_UTR
TATGTTACAATTGCAAAGCC+TGG 0.436181 8_3:+64421623 Msa1302080:CDS
TAGTATTCCTTCATCTGTCT+GGG 0.455311 8_3:+64420183 Msa1302080:CDS
GTGTTGTGTTCAAAATCTAA+TGG 0.470167 8_3:+64420118 Msa1302080:exon
CACCTGTCACCGTCAGTGAT+TGG 0.480273 8_3:-64421863 None:intergenic
GACAGAAGATTCAGAGGAGG+TGG 0.482508 8_3:-64420309 None:intergenic
TGCCAATCACTGACGGTGAC+AGG 0.491174 8_3:+64421861 Msa1302080:three_prime_UTR
TCCCAGACCCAGACAGATGA+AGG 0.512288 8_3:-64420190 None:intergenic
TCAGCTGCAAAAGAGATGAA+TGG 0.519432 8_3:+64421493 Msa1302080:CDS
TCAACCAAAATCTTCATCAA+AGG 0.523578 8_3:+64420350 Msa1302080:CDS
GAAATTGAAAGTGAAAGAGT+TGG 0.531902 8_3:-64420248 None:intergenic
CTGTTTGGTGAAGTTACTCA+AGG 0.549668 8_3:+64421254 Msa1302080:CDS
ATTTCTAGCATTGTGCCTTG+GGG 0.561081 8_3:+64421758 Msa1302080:three_prime_UTR
TGCATATATTTGGTTTGTCG+AGG 0.565736 8_3:+64421456 Msa1302080:CDS
GAGGAGGTGGAGCAAAAGCA+TGG 0.569253 8_3:-64420296 None:intergenic
TTTCTAGCATTGTGCCTTGG+GGG 0.582549 8_3:+64421759 Msa1302080:three_prime_UTR
TTTCGTTAAATGCACTTCAG+AGG 0.589643 8_3:-64421222 None:intergenic
TGAAGGAATACTACAATTTG+GGG 0.591870 8_3:-64420173 None:intergenic
GTGGAGCAAAAGCATGGAGA+AGG 0.598651 8_3:-64420290 None:intergenic
TGGAGAAGGTGCATAGCGAG+TGG 0.615709 8_3:-64420276 None:intergenic
CTAAAAGTGCCAATCACTGA+CGG 0.620131 8_3:+64421854 Msa1302080:three_prime_UTR
TGCACTTCAGAGGTTGAGAG+AGG 0.626279 8_3:-64421212 None:intergenic
ACATTCGACAGAAGATTCAG+AGG 0.629820 8_3:-64420315 None:intergenic
TGCAAAAGAGATGAATGGAA+AGG 0.634043 8_3:+64421498 Msa1302080:CDS
AGAAGGTGCATAGCGAGTGG+AGG 0.655325 8_3:-64420273 None:intergenic
GTATTGTATGCTTACTAGAG+TGG 0.660770 8_3:-64421711 None:intergenic
TTCGACAGAAGATTCAGAGG+AGG 0.703143 8_3:-64420312 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGGTAATTATAAATATATTT+TGG + chr8_3:64421274-64421293 Msa1302080:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_3:64421275-64421294 Msa1302080:intron 10.0%
!!! AGGTAATTATAAATATATTT+TGG + chr8_3:64421274-64421293 Msa1302080:intron 10.0%
!!! GGTAATTATAAATATATTTT+GGG + chr8_3:64421275-64421294 Msa1302080:intron 10.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_3:64420577-64420596 Msa1302080:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_3:64420578-64420597 Msa1302080:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_3:64420664-64420683 Msa1302080:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_3:64420996-64421015 Msa1302080:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_3:64421387-64421406 Msa1302080:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_3:64421419-64421438 Msa1302080:CDS 15.0%
!!! ATCAAATTGAATTAGAATTT+TGG + chr8_3:64420577-64420596 Msa1302080:intron 15.0%
!!! TCAAATTGAATTAGAATTTT+GGG + chr8_3:64420578-64420597 Msa1302080:intron 15.0%
!! ATACAACAAGATAAAATAAA+TGG + chr8_3:64420664-64420683 Msa1302080:intron 15.0%
!! AATTATATGAAAAAAGTCAT+TGG + chr8_3:64420996-64421015 Msa1302080:intron 15.0%
!!! TTTTTTATTGCTTTATTTCA+TGG + chr8_3:64421387-64421406 Msa1302080:intron 15.0%
!!! TTTTGATAGATAAAAAAACT+GGG + chr8_3:64421419-64421438 Msa1302080:CDS 15.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_3:64420989-64421008 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_3:64421323-64421342 Msa1302080:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_3:64421418-64421437 Msa1302080:CDS 20.0%
!!! TTTTCATATAATTAGATCGT+AGG - chr8_3:64420989-64421008 None:intergenic 20.0%
!!! TCATATTTGCTTTGAATTAA+TGG + chr8_3:64421323-64421342 Msa1302080:intron 20.0%
!!! CTTTTGATAGATAAAAAAAC+TGG + chr8_3:64421418-64421437 Msa1302080:CDS 20.0%
! ATGAAGGAATACTACAATTT+GGG - chr8_3:64420177-64420196 None:intergenic 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_3:64420702-64420721 Msa1302080:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_3:64420734-64420753 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_3:64420827-64420846 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_3:64420828-64420847 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_3:64420929-64420948 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_3:64421141-64421160 Msa1302080:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_3:64421338-64421357 Msa1302080:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_3:64421675-64421694 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_3:64421924-64421943 Msa1302080:three_prime_UTR 25.0%
! ATGAAGGAATACTACAATTT+GGG - chr8_3:64420177-64420196 None:intergenic 25.0%
! AGATTAATTTCTTGGAACTT+CGG + chr8_3:64420702-64420721 Msa1302080:intron 25.0%
! AATATTTGTTTAGTCTGAAC+TGG - chr8_3:64420734-64420753 None:intergenic 25.0%
!! TTTCAAATGTAAAGTGTTTC+GGG - chr8_3:64420827-64420846 None:intergenic 25.0%
!! CTTTCAAATGTAAAGTGTTT+CGG - chr8_3:64420828-64420847 None:intergenic 25.0%
!! AAATAAGCACGCATAATTTT+CGG - chr8_3:64420929-64420948 None:intergenic 25.0%
! ATAAATTATCCATTTCCACT+TGG + chr8_3:64421141-64421160 Msa1302080:intron 25.0%
!!! ATTAATGGAACTTCAAGTTT+TGG + chr8_3:64421338-64421357 Msa1302080:intron 25.0%
! TCATGTCAGTTAAAATTTGT+AGG - chr8_3:64421675-64421694 None:intergenic 25.0%
! CTATATTGAACAGTGTATTA+AGG + chr8_3:64421924-64421943 Msa1302080:three_prime_UTR 25.0%
GTGTTGTGTTCAAAATCTAA+TGG + chr8_3:64420118-64420137 Msa1302080:exon 30.0%
TGAAGGAATACTACAATTTG+GGG - chr8_3:64420176-64420195 None:intergenic 30.0%
GATGAAGGAATACTACAATT+TGG - chr8_3:64420178-64420197 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_3:64420251-64420270 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_3:64420347-64420366 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_3:64420350-64420369 Msa1302080:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_3:64420357-64420376 None:intergenic 30.0%
! TTATTGATTAAAGGGATAGG+GGG - chr8_3:64420390-64420409 None:intergenic 30.0%
! GTTATTGATTAAAGGGATAG+GGG - chr8_3:64420391-64420410 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_3:64420392-64420411 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_3:64420390-64420409 Msa1302080:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_3:64420391-64420410 Msa1302080:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_3:64420398-64420417 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_3:64420399-64420418 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_3:64420526-64420545 Msa1302080:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_3:64420598-64420617 Msa1302080:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_3:64420637-64420656 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_3:64420699-64420718 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_3:64420703-64420722 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_3:64420826-64420845 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_3:64420893-64420912 Msa1302080:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_3:64420936-64420955 Msa1302080:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_3:64420937-64420956 Msa1302080:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_3:64420940-64420959 Msa1302080:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_3:64420980-64420999 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_3:64420981-64421000 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG - chr8_3:64421054-64421073 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_3:64421068-64421087 Msa1302080:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG + chr8_3:64421289-64421308 Msa1302080:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_3:64421357-64421376 Msa1302080:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_3:64421446-64421465 Msa1302080:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_3:64421557-64421576 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_3:64421590-64421609 Msa1302080:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_3:64421644-64421663 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_3:64421848-64421867 None:intergenic 30.0%
GTGTTGTGTTCAAAATCTAA+TGG + chr8_3:64420118-64420137 Msa1302080:exon 30.0%
TGAAGGAATACTACAATTTG+GGG - chr8_3:64420176-64420195 None:intergenic 30.0%
GATGAAGGAATACTACAATT+TGG - chr8_3:64420178-64420197 None:intergenic 30.0%
GAAATTGAAAGTGAAAGAGT+TGG - chr8_3:64420251-64420270 None:intergenic 30.0%
!!! ATGAAGATTTTGGTTGAAGA+AGG - chr8_3:64420347-64420366 None:intergenic 30.0%
TCAACCAAAATCTTCATCAA+AGG + chr8_3:64420350-64420369 Msa1302080:CDS 30.0%
! GTTACCTTTGATGAAGATTT+TGG - chr8_3:64420357-64420376 None:intergenic 30.0%
! TTATTGATTAAAGGGATAGG+GGG - chr8_3:64420390-64420409 None:intergenic 30.0%
! GTTATTGATTAAAGGGATAG+GGG - chr8_3:64420391-64420410 None:intergenic 30.0%
! CGTTATTGATTAAAGGGATA+GGG - chr8_3:64420392-64420411 None:intergenic 30.0%
CCTATCCCTTTAATCAATAA+CGG + chr8_3:64420390-64420409 Msa1302080:intron 30.0%
CTATCCCTTTAATCAATAAC+GGG + chr8_3:64420391-64420410 Msa1302080:intron 30.0%
! GAAACCCGTTATTGATTAAA+GGG - chr8_3:64420398-64420417 None:intergenic 30.0%
! AGAAACCCGTTATTGATTAA+AGG - chr8_3:64420399-64420418 None:intergenic 30.0%
CATTAGAGAATGAGAATAGA+AGG + chr8_3:64420526-64420545 Msa1302080:intron 30.0%
GGGACAATGATAACAAATTA+AGG + chr8_3:64420598-64420617 Msa1302080:intron 30.0%
ACTAAATAGTAATACCAGAG+AGG - chr8_3:64420637-64420656 None:intergenic 30.0%
TTCCAAGAAATTAATCTGGT+CGG - chr8_3:64420699-64420718 None:intergenic 30.0%
GAAGTTCCAAGAAATTAATC+TGG - chr8_3:64420703-64420722 None:intergenic 30.0%
! TTCAAATGTAAAGTGTTTCG+GGG - chr8_3:64420826-64420845 None:intergenic 30.0%
! ATACAATTTTTATCTGCCTG+TGG + chr8_3:64420893-64420912 Msa1302080:intron 30.0%
TGCGTGCTTATTTAAAAATG+TGG + chr8_3:64420936-64420955 Msa1302080:intron 30.0%
GCGTGCTTATTTAAAAATGT+GGG + chr8_3:64420937-64420956 Msa1302080:intron 30.0%
TGCTTATTTAAAAATGTGGG+CGG + chr8_3:64420940-64420959 Msa1302080:intron 30.0%
AATTAGATCGTAGGTAACAA+GGG - chr8_3:64420980-64420999 None:intergenic 30.0%
TAATTAGATCGTAGGTAACA+AGG - chr8_3:64420981-64421000 None:intergenic 30.0%
!!! AATACAACTGTTTTTGTGTG+TGG - chr8_3:64421054-64421073 None:intergenic 30.0%
TGTATTGCATATATGTAAGC+AGG + chr8_3:64421068-64421087 Msa1302080:intron 30.0%
!! TATTTTGGGTGTTTTCTGAA+AGG + chr8_3:64421289-64421308 Msa1302080:intron 30.0%
TTGGAAATCTTGAGTGTTTA+AGG + chr8_3:64421357-64421376 Msa1302080:intron 30.0%
!! CTCTAGGATTTGCATATATT+TGG + chr8_3:64421446-64421465 Msa1302080:CDS 30.0%
GTCATCAACATTGTATAGTA+TGG - chr8_3:64421557-64421576 None:intergenic 30.0%
!!! TTTGTGGTGCAGTTTTTTTA+TGG + chr8_3:64421590-64421609 Msa1302080:intron 30.0%
!! TTCAAACTTTTTGATGATCC+AGG - chr8_3:64421644-64421663 None:intergenic 30.0%
! ATTGGCACTTTTAGATCAAA+AGG - chr8_3:64421848-64421867 None:intergenic 30.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_3:64420131-64420150 Msa1302080:exon 35.0%
TAGTATTCCTTCATCTGTCT+GGG + chr8_3:64420183-64420202 Msa1302080:CDS 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_3:64420393-64420412 None:intergenic 35.0%
! ACGGGTTTCTTTCAATTTTG+TGG + chr8_3:64420409-64420428 Msa1302080:intron 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_3:64420535-64420554 Msa1302080:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_3:64420694-64420713 Msa1302080:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_3:64421126-64421145 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_3:64421171-64421190 Msa1302080:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG - chr8_3:64421225-64421244 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+TGG + chr8_3:64421239-64421258 Msa1302080:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_3:64421456-64421475 Msa1302080:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_3:64421498-64421517 Msa1302080:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_3:64421623-64421642 Msa1302080:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_3:64421714-64421733 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_3:64421757-64421776 Msa1302080:three_prime_UTR 35.0%
!! AATCTAATGGCACTTGTTGT+TGG + chr8_3:64420131-64420150 Msa1302080:exon 35.0%
TAGTATTCCTTCATCTGTCT+GGG + chr8_3:64420183-64420202 Msa1302080:CDS 35.0%
! CCGTTATTGATTAAAGGGAT+AGG - chr8_3:64420393-64420412 None:intergenic 35.0%
! ACGGGTTTCTTTCAATTTTG+TGG + chr8_3:64420409-64420428 Msa1302080:intron 35.0%
ATGAGAATAGAAGGCTCTTA+CGG + chr8_3:64420535-64420554 Msa1302080:intron 35.0%
TTCCGACCAGATTAATTTCT+TGG + chr8_3:64420694-64420713 Msa1302080:intron 35.0%
! TTTATTACGACAAGCATTGC+AGG - chr8_3:64421126-64421145 None:intergenic 35.0%
TAGTTGTACTGAAGTCTTCA+TGG + chr8_3:64421171-64421190 Msa1302080:intron 35.0%
TTTCGTTAAATGCACTTCAG+AGG - chr8_3:64421225-64421244 None:intergenic 35.0%
!!! ACGAAAGTGTTTTCACTGTT+TGG + chr8_3:64421239-64421258 Msa1302080:CDS 35.0%
!! TGCATATATTTGGTTTGTCG+AGG + chr8_3:64421456-64421475 Msa1302080:CDS 35.0%
TGCAAAAGAGATGAATGGAA+AGG + chr8_3:64421498-64421517 Msa1302080:CDS 35.0%
TATGTTACAATTGCAAAGCC+TGG + chr8_3:64421623-64421642 Msa1302080:CDS 35.0%
GTATTGTATGCTTACTAGAG+TGG - chr8_3:64421714-64421733 None:intergenic 35.0%
!! AATTTCTAGCATTGTGCCTT+GGG + chr8_3:64421757-64421776 Msa1302080:three_prime_UTR 35.0%
GTAGTATTCCTTCATCTGTC+TGG + chr8_3:64420182-64420201 Msa1302080:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_3:64420318-64420337 None:intergenic 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_3:64420822-64420841 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_3:64420850-64420869 Msa1302080:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_3:64421023-64421042 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_3:64421024-64421043 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_3:64421153-64421172 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_3:64421159-64421178 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_3:64421175-64421194 Msa1302080:intron 40.0%
! CTGTTTGGTGAAGTTACTCA+AGG + chr8_3:64421254-64421273 Msa1302080:CDS 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_3:64421430-64421449 Msa1302080:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_3:64421493-64421512 Msa1302080:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_3:64421565-64421584 Msa1302080:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_3:64421594-64421613 Msa1302080:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_3:64421756-64421775 Msa1302080:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_3:64421758-64421777 Msa1302080:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_3:64421854-64421873 Msa1302080:three_prime_UTR 40.0%
GTAGTATTCCTTCATCTGTC+TGG + chr8_3:64420182-64420201 Msa1302080:CDS 40.0%
ACATTCGACAGAAGATTCAG+AGG - chr8_3:64420318-64420337 None:intergenic 40.0%
! AATGTAAAGTGTTTCGGGGT+TGG - chr8_3:64420822-64420841 None:intergenic 40.0%
! AACACAGTTTTTGTGCTGTC+CGG + chr8_3:64420850-64420869 Msa1302080:intron 40.0%
TGATACAAGAGTGGCAGAAT+GGG - chr8_3:64421023-64421042 None:intergenic 40.0%
ATGATACAAGAGTGGCAGAA+TGG - chr8_3:64421024-64421043 None:intergenic 40.0%
TACTTAACGCCAAGTGGAAA+TGG - chr8_3:64421153-64421172 None:intergenic 40.0%
TACAACTACTTAACGCCAAG+TGG - chr8_3:64421159-64421178 None:intergenic 40.0%
TGTACTGAAGTCTTCATGGT+CGG + chr8_3:64421175-64421194 Msa1302080:intron 40.0%
! CTGTTTGGTGAAGTTACTCA+AGG + chr8_3:64421254-64421273 Msa1302080:CDS 40.0%
AAAAAAACTGGGCAGTCTCT+AGG + chr8_3:64421430-64421449 Msa1302080:CDS 40.0%
TCAGCTGCAAAAGAGATGAA+TGG + chr8_3:64421493-64421512 Msa1302080:CDS 40.0%
!! AATGTTGATGACATGTGAGC+TGG + chr8_3:64421565-64421584 Msa1302080:intron 40.0%
!!! TGGTGCAGTTTTTTTATGGC+AGG + chr8_3:64421594-64421613 Msa1302080:intron 40.0%
!! GAATTTCTAGCATTGTGCCT+TGG + chr8_3:64421756-64421775 Msa1302080:three_prime_UTR 40.0%
!! ATTTCTAGCATTGTGCCTTG+GGG + chr8_3:64421758-64421777 Msa1302080:three_prime_UTR 40.0%
!! CTAAAAGTGCCAATCACTGA+CGG + chr8_3:64421854-64421873 Msa1302080:three_prime_UTR 40.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_3:64420315-64420334 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_3:64420790-64420809 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_3:64420791-64420810 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_3:64420912-64420931 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_3:64421032-64421051 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG - chr8_3:64421116-64421135 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_3:64421122-64421141 None:intergenic 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_3:64421574-64421593 Msa1302080:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_3:64421759-64421778 Msa1302080:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_3:64421776-64421795 None:intergenic 45.0%
AATGTGCAAGTGCAGCATTG+GGG - chr8_3:64420072-64420091 None:intergenic 45.0%
GAATGTGCAAGTGCAGCATT+GGG - chr8_3:64420073-64420092 None:intergenic 45.0%
AGAATGTGCAAGTGCAGCAT+TGG - chr8_3:64420074-64420093 None:intergenic 45.0%
TTCGACAGAAGATTCAGAGG+AGG - chr8_3:64420315-64420334 None:intergenic 45.0%
TCAGAACCAACAATGCATGC+GGG - chr8_3:64420790-64420809 None:intergenic 45.0%
CTCAGAACCAACAATGCATG+CGG - chr8_3:64420791-64420810 None:intergenic 45.0%
! TTTCGGAAGTGTACTTCCAC+AGG - chr8_3:64420912-64420931 None:intergenic 45.0%
GATGTGCGATGATACAAGAG+TGG - chr8_3:64421032-64421051 None:intergenic 45.0%
! CAAGCATTGCAGGAAGGAAA+CGG - chr8_3:64421116-64421135 None:intergenic 45.0%
! TTACGACAAGCATTGCAGGA+AGG - chr8_3:64421122-64421141 None:intergenic 45.0%
!!! GACATGTGAGCTGGTTTTTG+TGG + chr8_3:64421574-64421593 Msa1302080:intron 45.0%
! TTTCTAGCATTGTGCCTTGG+GGG + chr8_3:64421759-64421778 Msa1302080:three_prime_UTR 45.0%
TTTACCAAAACGAACCCCCA+AGG - chr8_3:64421776-64421795 None:intergenic 45.0%
TTCCTTCATCTGTCTGGGTC+TGG + chr8_3:64420188-64420207 Msa1302080:CDS 50.0%
TCCTTCATCTGTCTGGGTCT+GGG + chr8_3:64420189-64420208 Msa1302080:CDS 50.0%
! GTGGAGCAAAAGCATGGAGA+AGG - chr8_3:64420293-64420312 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_3:64420312-64420331 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_3:64420789-64420808 Msa1302080:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_3:64420872-64420891 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG - chr8_3:64421215-64421234 None:intergenic 50.0%
TTCCTTCATCTGTCTGGGTC+TGG + chr8_3:64420188-64420207 Msa1302080:CDS 50.0%
TCCTTCATCTGTCTGGGTCT+GGG + chr8_3:64420189-64420208 Msa1302080:CDS 50.0%
! GTGGAGCAAAAGCATGGAGA+AGG - chr8_3:64420293-64420312 None:intergenic 50.0%
GACAGAAGATTCAGAGGAGG+TGG - chr8_3:64420312-64420331 None:intergenic 50.0%
!! CGCATGCATTGTTGGTTCTG+AGG + chr8_3:64420789-64420808 Msa1302080:intron 50.0%
! CTTGTCCAGAAGTGCACATC+CGG - chr8_3:64420872-64420891 None:intergenic 50.0%
TGCACTTCAGAGGTTGAGAG+AGG - chr8_3:64421215-64421234 None:intergenic 50.0%
TCCCAGACCCAGACAGATGA+AGG - chr8_3:64420193-64420212 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_3:64420276-64420295 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_3:64420279-64420298 None:intergenic 55.0%
GAGGAGGTGGAGCAAAAGCA+TGG - chr8_3:64420299-64420318 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_3:64420620-64420639 Msa1302080:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_3:64420785-64420804 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_3:64420786-64420805 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_3:64421185-64421204 Msa1302080:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_3:64421186-64421205 Msa1302080:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_3:64421201-64421220 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_3:64421769-64421788 Msa1302080:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_3:64421861-64421880 Msa1302080:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_3:64421866-64421885 None:intergenic 55.0%
TCCCAGACCCAGACAGATGA+AGG - chr8_3:64420193-64420212 None:intergenic 55.0%
! AGAAGGTGCATAGCGAGTGG+AGG - chr8_3:64420276-64420295 None:intergenic 55.0%
! TGGAGAAGGTGCATAGCGAG+TGG - chr8_3:64420279-64420298 None:intergenic 55.0%
GAGGAGGTGGAGCAAAAGCA+TGG - chr8_3:64420299-64420318 None:intergenic 55.0%
! GTGCTAGCTCTTTGCCTCTC+TGG + chr8_3:64420620-64420639 Msa1302080:intron 55.0%
ACCAACAATGCATGCGGGCA+GGG - chr8_3:64420785-64420804 None:intergenic 55.0%
AACCAACAATGCATGCGGGC+AGG - chr8_3:64420786-64420805 None:intergenic 55.0%
TCTTCATGGTCGGCCAATGC+AGG + chr8_3:64421185-64421204 Msa1302080:intron 55.0%
CTTCATGGTCGGCCAATGCA+GGG + chr8_3:64421186-64421205 Msa1302080:intron 55.0%
TGAGAGAGGAAGCCCTGCAT+TGG - chr8_3:64421201-64421220 None:intergenic 55.0%
! TGTGCCTTGGGGGTTCGTTT+TGG + chr8_3:64421769-64421788 Msa1302080:three_prime_UTR 55.0%
TGCCAATCACTGACGGTGAC+AGG + chr8_3:64421861-64421880 Msa1302080:three_prime_UTR 55.0%
CACCTGTCACCGTCAGTGAT+TGG - chr8_3:64421866-64421885 None:intergenic 55.0%
CAACAATGCATGCGGGCAGG+GGG - chr8_3:64420783-64420802 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_3:64420784-64420803 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_3:64420864-64420883 Msa1302080:intron 60.0%
CAACAATGCATGCGGGCAGG+GGG - chr8_3:64420783-64420802 None:intergenic 60.0%
CCAACAATGCATGCGGGCAG+GGG - chr8_3:64420784-64420803 None:intergenic 60.0%
GCTGTCCGGATGTGCACTTC+TGG + chr8_3:64420864-64420883 Msa1302080:intron 60.0%
ATGCATGCGGGCAGGGGGTT+AGG - chr8_3:64420778-64420797 None:intergenic 65.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_3:64420781-64420800 Msa1302080:intron 65.0%
ATGCATGCGGGCAGGGGGTT+AGG - chr8_3:64420778-64420797 None:intergenic 65.0%
CCCCTGCCCGCATGCATTGT+TGG + chr8_3:64420781-64420800 Msa1302080:intron 65.0%
CATGCGGGCAGGGGGTTAGG+AGG - chr8_3:64420775-64420794 None:intergenic 70.0%
CATGCGGGCAGGGGGTTAGG+AGG - chr8_3:64420775-64420794 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 64420072 64421953 64420072 ID=Msa1302080;Name=Msa1302080
chr8_3 mRNA 64420072 64421953 64420072 ID=Msa1302080-mRNA-1;Parent=Msa1302080;Name=Msa1302080-mRNA-1;_AED=0.03;_eAED=0.03;_QI=65|1|1|1|1|1|4|268|164
chr8_3 exon 64420072 64420371 64420072 ID=Msa1302080-mRNA-1:exon:19426;Parent=Msa1302080-mRNA-1
chr8_3 exon 64421207 64421275 64421207 ID=Msa1302080-mRNA-1:exon:19427;Parent=Msa1302080-mRNA-1
chr8_3 exon 64421413 64421519 64421413 ID=Msa1302080-mRNA-1:exon:19428;Parent=Msa1302080-mRNA-1
chr8_3 exon 64421602 64421953 64421602 ID=Msa1302080-mRNA-1:exon:19429;Parent=Msa1302080-mRNA-1
chr8_3 five_prime_UTR 64420072 64420136 64420072 ID=Msa1302080-mRNA-1:five_prime_utr;Parent=Msa1302080-mRNA-1
chr8_3 CDS 64420137 64420371 64420137 ID=Msa1302080-mRNA-1:cds;Parent=Msa1302080-mRNA-1
chr8_3 CDS 64421207 64421275 64421207 ID=Msa1302080-mRNA-1:cds;Parent=Msa1302080-mRNA-1
chr8_3 CDS 64421413 64421519 64421413 ID=Msa1302080-mRNA-1:cds;Parent=Msa1302080-mRNA-1
chr8_3 CDS 64421602 64421685 64421602 ID=Msa1302080-mRNA-1:cds;Parent=Msa1302080-mRNA-1
chr8_3 three_prime_UTR 64421686 64421953 64421686 ID=Msa1302080-mRNA-1:three_prime_utr;Parent=Msa1302080-mRNA-1
Gene Sequence

>Msa1302080

ATGGCACTTGTTGTTGGTAGTAGTACTATATGCAAACCCCAAATTGTAGTATTCCTTCATCTGTCTGGGTCTGGGAGTTTGAAGAAACAAACAGTTTCGAAAATCACATTTCCAACTCTTTCACTTTCAATTTCTACCTCCACTCGCTATGCACCTTCTCCATGCTTTTGCTCCACCTCCTCTGAATCTTCTGTCGAATGTAATACGCCTTCTTCAACCAAAATCTTCATCAAAGGGCTTCCTCTCTCAACCTCTGAAGTGCATTTAACGAAAGTGTTTTCACTGTTTGGTGAAGTTACTCAAGTCAAGCTTTTGATAGATAAAAAAACTGGGCAGTCTCTAGGATTTGCATATATTTGGTTTGTCGAGGAAGAGTCTGCACAATCAGCTGCAAAAGAGATGAATGGAAAGTTTTTTTATGGCAGGTTTATTTATGTTACAATTGCAAAGCCTGGATCATCAAAAAGTTTGAAGAGAAAAGCCTACAAATTTTAA
Protein sequence

>Msa1302080

MALVVGSSTICKPQIVVFLHLSGSGSLKKQTVSKITFPTLSLSISTSTRYAPSPCFCSTSSESSVECNTPSSTKIFIKGLPLSTSEVHLTKVFSLFGEVTQVKLLIDKKTGQSLGFAYIWFVEEESAQSAAKEMNGKFFYGRFIYVTIAKPGSSKSLKRKAYKF*