AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1271710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271710 MtrunA17_Chr8g0335791 100.000 149 0 0 1 149 1 149 3.10e-108 304
Msa1271710 MtrunA17_Chr4g0057901 64.865 148 51 1 3 149 83 230 1.19e-66 202
Msa1271710 MtrunA17_Chr8g0388131 59.184 147 59 1 3 148 85 231 2.88e-62 191
Msa1271710 MtrunA17_Chr8g0344421 34.074 135 86 1 3 134 34 168 2.50e-24 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1271710 AT4G09320.1 83.673 147 24 0 3 149 2 148 1.81e-91 262
Msa1271710 AT5G63310.1 63.514 148 53 1 3 149 84 231 9.57e-67 202
Msa1271710 AT4G11010.1 59.184 147 59 1 3 148 89 235 2.87e-62 191
Msa1271710 AT4G23900.1 59.864 147 58 1 3 148 88 234 3.32e-62 191
Msa1271710 AT4G23895.3 59.864 147 58 1 3 148 318 464 5.81e-60 192
Msa1271710 AT1G17410.1 35.556 135 84 1 3 134 33 167 1.46e-22 88.6
Msa1271710 AT1G17410.2 35.385 130 81 1 8 134 1 130 2.33e-21 84.3
Msa1271710 AT1G17410.3 34.066 91 60 0 8 98 1 91 3.95e-12 60.1

Find 73 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATCTGAACTTGAAGTTT+TGG 0.248011 8_3:+5162081 Msa1271710:three_prime_UTR
TTCCAGCAAAGGATCAAAAT+AGG 0.255262 8_3:-5161970 None:intergenic
GAAAGCGCCAACAAGGAAAT+TGG 0.280936 8_3:+5161853 Msa1271710:CDS
CCCTGTTGTTGCCATGATTT+GGG 0.284074 8_3:+5161122 Msa1271710:CDS
GCCCTGTTGTTGCCATGATT+TGG 0.311298 8_3:+5161121 Msa1271710:CDS
GCCATCTTGATTTCTCTGTT+TGG 0.335715 8_3:-5159956 None:intergenic
GCAAATTCCCGATCAACTTC+AGG 0.343910 8_3:-5161947 None:intergenic
CCACAAACCAATTTCCTTGT+TGG 0.361994 8_3:-5161860 None:intergenic
TGTGGTGGTGTGAGAGGAAA+GGG 0.366610 8_3:-5159886 None:intergenic
TATCAGCAGGTTTGAGAAGA+AGG 0.368456 8_3:+5160422 Msa1271710:CDS
TCCCAAATCATGGCAACAAC+AGG 0.371495 8_3:-5161123 None:intergenic
GGTGACTTTGCTGTTGAGAT+TGG 0.374163 8_3:+5161225 Msa1271710:CDS
CCGATAAATTAAAATAATAA+TGG 0.378171 8_3:-5162172 None:intergenic
TGAAAGTGAAATTAGGGTTA+AGG 0.384796 8_3:+5159838 Msa1271710:five_prime_UTR
TGCCATGCAGCAGGTCCCTC+AGG 0.393989 8_3:-5161886 None:intergenic
TTTATGAGTGTTTGATGCCT+TGG 0.398099 8_3:+5161994 Msa1271710:three_prime_UTR
AGGCTGCTTTGCCATGCAGC+AGG 0.417205 8_3:-5161895 None:intergenic
TCTCTGTTTGGTATTGAGTT+CGG 0.423596 8_3:-5159944 None:intergenic
TGATGCAGGAACGTTATTCA+TGG 0.430285 8_3:+5161817 Msa1271710:intron
GTTGCTGTTGTTGCTGTGTT+GGG 0.437459 8_3:-5159922 None:intergenic
GACAGATCCCTTAGGTTGGT+TGG 0.438255 8_3:+5162312 Msa1271710:three_prime_UTR
CTCAGATTGAGCAGGGTTTG+TGG 0.440349 8_3:-5161187 None:intergenic
ACAACTGGCAGAAAGATTAT+TGG 0.443417 8_3:+5161162 Msa1271710:CDS
GGGAAGAATGTTGTGACAAC+TGG 0.448668 8_3:+5161147 Msa1271710:CDS
TCCAGCAAAGGATCAAAATA+GGG 0.449640 8_3:-5161969 None:intergenic
GGTTGCTGTTGTTGCTGTGT+TGG 0.450078 8_3:-5159923 None:intergenic
CCAACAAGGAAATTGGTTTG+TGG 0.455431 8_3:+5161860 Msa1271710:CDS
AAGTCACCACGAATAGTTCC+AGG 0.456893 8_3:-5161210 None:intergenic
GTGACTTTGCTGTTGAGATT+GGG 0.461283 8_3:+5161226 Msa1271710:CDS
ATTCTTCCCTTCCCAAATCA+TGG 0.462620 8_3:-5161133 None:intergenic
TGGTGGTGTGAGAGGAAAGG+GGG 0.468982 8_3:-5159884 None:intergenic
AGATAAACCTGAAGTTGATC+GGG 0.475647 8_3:+5161940 Msa1271710:three_prime_UTR
AGTCCTCTTTGAACTCCATC+AGG 0.479734 8_3:-5160004 None:intergenic
GTGTGGTGGTGTGAGAGGAA+AGG 0.488846 8_3:-5159887 None:intergenic
TTGGTAGAGTACATTGTCTC+TGG 0.492742 8_3:+5161099 Msa1271710:CDS
TCTGAGCCTGGAACTATTCG+TGG 0.495457 8_3:+5161204 Msa1271710:CDS
AAGATAAACCTGAAGTTGAT+CGG 0.501734 8_3:+5161939 Msa1271710:exon
TGATAGTTCCAACCAACCTA+AGG 0.504958 8_3:-5162320 None:intergenic
TCTACCAATCCGCTGAAGAA+AGG 0.506686 8_3:-5161084 None:intergenic
AAGAAGGGTTTCTACTTGAA+AGG 0.507643 8_3:+5160438 Msa1271710:CDS
AACCCTGCTCAATCTGAGCC+TGG 0.508261 8_3:+5161192 Msa1271710:CDS
GTTCCAGGCTCAGATTGAGC+AGG 0.508318 8_3:-5161195 None:intergenic
TATAAGTGCAACCACATCAA+TGG 0.514650 8_3:-5162144 None:intergenic
TTTGCATAGACAGATCCCTT+AGG 0.521901 8_3:+5162304 Msa1271710:three_prime_UTR
TCTGCAAAGCCTTTCTTCAG+CGG 0.530736 8_3:+5161075 Msa1271710:CDS
AAAGCCTTTCTTCAGCGGAT+TGG 0.536682 8_3:+5161080 Msa1271710:CDS
ATCAGCAGGTTTGAGAAGAA+GGG 0.548918 8_3:+5160423 Msa1271710:CDS
ATTGGTTTGTGGTTCCCTGA+GGG 0.548939 8_3:+5161871 Msa1271710:CDS
TTCATCATGATCAAGCCTGA+TGG 0.549192 8_3:+5159989 Msa1271710:CDS
CATAGACAGATCCCTTAGGT+TGG 0.549764 8_3:+5162308 Msa1271710:three_prime_UTR
GGCAAAGCAGCCTCCACTCG+TGG 0.563561 8_3:+5161905 Msa1271710:CDS
GGTGGTGTGAGAGGAAAGGG+GGG 0.566114 8_3:-5159883 None:intergenic
TTCCAGGCTCAGATTGAGCA+GGG 0.574819 8_3:-5161194 None:intergenic
GGAGAGAGTGTGATGTGTGG+TGG 0.583086 8_3:-5159901 None:intergenic
CACTCATAAAGTTCCAGCAA+AGG 0.585680 8_3:-5161981 None:intergenic
GCCATGCAGCAGGTCCCTCA+GGG 0.586568 8_3:-5161885 None:intergenic
GATAGTTCCAACCAACCTAA+GGG 0.588934 8_3:-5162319 None:intergenic
AGGTTGGTGAGATTATCAGC+AGG 0.589081 8_3:+5160409 Msa1271710:intron
TTCTGTTGAAAGCGCCAACA+AGG 0.589117 8_3:+5161846 Msa1271710:CDS
GTGATGTGTGGTGGTGTGAG+AGG 0.590503 8_3:-5159892 None:intergenic
GTTGTTGCCATGATTTGGGA+AGG 0.598008 8_3:+5161126 Msa1271710:CDS
ACTTTGCTGTTGAGATTGGG+AGG 0.598896 8_3:+5161229 Msa1271710:CDS
ACTCGTAAATCCACGAGTGG+AGG 0.617859 8_3:-5161915 None:intergenic
CCCAAATCATGGCAACAACA+GGG 0.626477 8_3:-5161122 None:intergenic
GTGGTGGTGTGAGAGGAAAG+GGG 0.627876 8_3:-5159885 None:intergenic
ACCAAACAGAGAAATCAAGA+TGG 0.632343 8_3:+5159955 Msa1271710:exon
TTGGGAGAGAGTGTGATGTG+TGG 0.641202 8_3:-5159904 None:intergenic
TTGTTGCCATGATTTGGGAA+GGG 0.646062 8_3:+5161127 Msa1271710:CDS
AATTGGTTTGTGGTTCCCTG+AGG 0.646541 8_3:+5161870 Msa1271710:CDS
AAGCCTGATGGAGTTCAAAG+AGG 0.651287 8_3:+5160001 Msa1271710:CDS
GATACTTAAAACTTGTGTTG+TGG 0.652801 8_3:+5162206 Msa1271710:three_prime_UTR
TAGACTTAACATCAAAACCA+AGG 0.662366 8_3:-5162011 None:intergenic
TTTACTCGTAAATCCACGAG+TGG 0.672996 8_3:-5161918 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAAAATATTTGAAG+TGG + chr8_3:5160193-5160212 Msa1271710:intron 10.0%
!!! TTTTTTAAAATATTTGAAGT+GGG + chr8_3:5160194-5160213 Msa1271710:intron 10.0%
!!! CCATTATTATTTTAATTTAT+CGG + chr8_3:5162172-5162191 Msa1271710:three_prime_UTR 10.0%
!!! AAAAAAAACGAAATTTTGAT+AGG - chr8_3:5160176-5160195 None:intergenic 15.0%
!!! ATTCTGGATTTTAAAATATT+TGG + chr8_3:5160899-5160918 Msa1271710:intron 15.0%
!!! CAGTTAAAATTTAAAACAAA+TGG - chr8_3:5161308-5161327 None:intergenic 15.0%
!!! TTGTTTTGAAAAAAATATCA+AGG - chr8_3:5161442-5161461 None:intergenic 15.0%
!! CCGATAAATTAAAATAATAA+TGG - chr8_3:5162175-5162194 None:intergenic 15.0%
!! CAGATAATAATAACCAAATA+AGG - chr8_3:5160139-5160158 None:intergenic 20.0%
!! TTTAGCTAAATTTATTTGTG+TGG + chr8_3:5160317-5160336 Msa1271710:intron 20.0%
!!! TTTGATACTTCTAAAATTTC+AGG + chr8_3:5160873-5160892 Msa1271710:intron 20.0%
!! CAAAATAAAAAATGAACGAT+AGG - chr8_3:5162116-5162135 None:intergenic 20.0%
!! GTTTTGTGAAAGTGAAATTA+GGG + chr8_3:5159832-5159851 Msa1271710:five_prime_UTR 25.0%
! GAATTGTGAATCAAAAAAAG+AGG - chr8_3:5160051-5160070 None:intergenic 25.0%
! CGTAATAAATCATCCTTATT+TGG + chr8_3:5160123-5160142 Msa1271710:intron 25.0%
! ACTAAAAGTTAGTATGCTAA+GGG - chr8_3:5160467-5160486 None:intergenic 25.0%
! AACTAAAAGTTAGTATGCTA+AGG - chr8_3:5160468-5160487 None:intergenic 25.0%
! CTTTGAATTTCAAAACAATC+AGG - chr8_3:5160802-5160821 None:intergenic 25.0%
!!! TGTTTTGAAATTCAAAGTTG+AGG + chr8_3:5160805-5160824 Msa1271710:intron 25.0%
! TTGAAAATCAACATTTGGAA+AGG - chr8_3:5160835-5160854 None:intergenic 25.0%
! TACACTTGAAAATCAACATT+TGG - chr8_3:5160840-5160859 None:intergenic 25.0%
!!! CACTTTGAAATTATTTGTGA+TGG + chr8_3:5160969-5160988 Msa1271710:intron 25.0%
!!! AGGTTTGAAATTTTTGAATG+TGG + chr8_3:5161017-5161036 Msa1271710:intron 25.0%
! TAAAATCAAGTCTTAAGAAC+AGG - chr8_3:5161400-5161419 None:intergenic 25.0%
!!! GTTTTGATTGACTTAAAACA+TGG - chr8_3:5161476-5161495 None:intergenic 25.0%
! ATAAAACTTAGATAAGAGCT+TGG + chr8_3:5161536-5161555 Msa1271710:intron 25.0%
!! CAAGTTTTCAAATCAAACAA+TGG - chr8_3:5161678-5161697 None:intergenic 25.0%
!!! CTTGATTTTAAGTTACAGAA+TGG + chr8_3:5161774-5161793 Msa1271710:intron 25.0%
!!! ATTTTAATCACTATTGATGC+AGG + chr8_3:5161803-5161822 Msa1271710:intron 25.0%
!! TTAATCTGAACTTGAAGTTT+TGG + chr8_3:5162081-5162100 Msa1271710:three_prime_UTR 25.0%
!!! ATTTTAATTTATCGGTTGTG+AGG + chr8_3:5162180-5162199 Msa1271710:three_prime_UTR 25.0%
!!! GGTGTTTTGTTGTATAATTT+TGG + chr8_3:5162227-5162246 Msa1271710:three_prime_UTR 25.0%
!! GCAAATGTGTATTCTAAAAA+AGG - chr8_3:5162289-5162308 None:intergenic 25.0%
! CGTTTTGTGAAAGTGAAATT+AGG + chr8_3:5159831-5159850 Msa1271710:five_prime_UTR 30.0%
TGAAAGTGAAATTAGGGTTA+AGG + chr8_3:5159838-5159857 Msa1271710:five_prime_UTR 30.0%
ATGCTTGACTGTTTAGAAAA+TGG + chr8_3:5160254-5160273 Msa1271710:intron 30.0%
TGCTTGACTGTTTAGAAAAT+GGG + chr8_3:5160255-5160274 Msa1271710:intron 30.0%
!! TAGAAAATGGGTTGATTTGT+TGG + chr8_3:5160267-5160286 Msa1271710:intron 30.0%
TTTGTTTGATGCTGAAAGTT+TGG + chr8_3:5160358-5160377 Msa1271710:intron 30.0%
GACTGAAAAATTTGTGTTTC+AGG + chr8_3:5160389-5160408 Msa1271710:intron 30.0%
! GAAAAATTTGTGTTTCAGGT+TGG + chr8_3:5160393-5160412 Msa1271710:intron 30.0%
CTAACAATCACTCACTTATT+GGG - chr8_3:5160716-5160735 None:intergenic 30.0%
TCTAACAATCACTCACTTAT+TGG - chr8_3:5160717-5160736 None:intergenic 30.0%
! CTAAAATTTCAGGTCAATTC+TGG + chr8_3:5160883-5160902 Msa1271710:intron 30.0%
! TGATTGACTTAAAACATGGA+AGG - chr8_3:5161472-5161491 None:intergenic 30.0%
AAGTATCATTGTTAGACATG+AGG - chr8_3:5161498-5161517 None:intergenic 30.0%
TATCCTTACCAATAGCTAAT+AGG + chr8_3:5161559-5161578 Msa1271710:intron 30.0%
AATAAGCACCTATTAGCTAT+TGG - chr8_3:5161570-5161589 None:intergenic 30.0%
AATAGATGATGTTTGATGCT+GGG - chr8_3:5161621-5161640 None:intergenic 30.0%
AAATAGATGATGTTTGATGC+TGG - chr8_3:5161622-5161641 None:intergenic 30.0%
AGCATCAAACATCATCTATT+TGG + chr8_3:5161621-5161640 Msa1271710:intron 30.0%
!! GAGCACTTTTGATTAGTTAA+GGG - chr8_3:5161736-5161755 None:intergenic 30.0%
!! AGAGCACTTTTGATTAGTTA+AGG - chr8_3:5161737-5161756 None:intergenic 30.0%
! AAGATAAACCTGAAGTTGAT+CGG + chr8_3:5161939-5161958 Msa1271710:exon 30.0%
TAGACTTAACATCAAAACCA+AGG - chr8_3:5162014-5162033 None:intergenic 30.0%
GATACTTAAAACTTGTGTTG+TGG + chr8_3:5162206-5162225 Msa1271710:three_prime_UTR 30.0%
!! TGTTGTATAATTTTGGTACG+TGG + chr8_3:5162234-5162253 Msa1271710:three_prime_UTR 30.0%
! TCTCTGTTTGGTATTGAGTT+CGG - chr8_3:5159947-5159966 None:intergenic 35.0%
ACCAAACAGAGAAATCAAGA+TGG + chr8_3:5159955-5159974 Msa1271710:exon 35.0%
! AAAAAAGAGGTGTGAGAGAA+GGG - chr8_3:5160038-5160057 None:intergenic 35.0%
! AAAAAAAGAGGTGTGAGAGA+AGG - chr8_3:5160039-5160058 None:intergenic 35.0%
AAGAAGGGTTTCTACTTGAA+AGG + chr8_3:5160438-5160457 Msa1271710:CDS 35.0%
TGATCAGAGAACAAAACAAC+AGG - chr8_3:5160509-5160528 None:intergenic 35.0%
TGAGATCCATTTCCAAAGTT+TGG + chr8_3:5160675-5160694 Msa1271710:intron 35.0%
GAGATCCATTTCCAAAGTTT+GGG + chr8_3:5160676-5160695 Msa1271710:intron 35.0%
! ACATATCAATTGAGAAGTGC+TGG - chr8_3:5160758-5160777 None:intergenic 35.0%
ACAACTGGCAGAAAGATTAT+TGG + chr8_3:5161162-5161181 Msa1271710:CDS 35.0%
CGTCTAGTATAGTGAATTGT+AGG + chr8_3:5161366-5161385 Msa1271710:intron 35.0%
ATATCAAGGTGACTTACATG+AGG - chr8_3:5161428-5161447 None:intergenic 35.0%
! AGATAAACCTGAAGTTGATC+GGG + chr8_3:5161940-5161959 Msa1271710:three_prime_UTR 35.0%
TCCAGCAAAGGATCAAAATA+GGG - chr8_3:5161972-5161991 None:intergenic 35.0%
TTCCAGCAAAGGATCAAAAT+AGG - chr8_3:5161973-5161992 None:intergenic 35.0%
TTTATGAGTGTTTGATGCCT+TGG + chr8_3:5161994-5162013 Msa1271710:three_prime_UTR 35.0%
!!! CTGAACTTGAAGTTTTGGTT+TGG + chr8_3:5162086-5162105 Msa1271710:three_prime_UTR 35.0%
TATAAGTGCAACCACATCAA+TGG - chr8_3:5162147-5162166 None:intergenic 35.0%
AGGAAAGGGGGGTTTATATA+TGG - chr8_3:5159875-5159894 None:intergenic 40.0%
GCCATCTTGATTTCTCTGTT+TGG - chr8_3:5159959-5159978 None:intergenic 40.0%
TTCATCATGATCAAGCCTGA+TGG + chr8_3:5159989-5160008 Msa1271710:CDS 40.0%
TATCAGCAGGTTTGAGAAGA+AGG + chr8_3:5160422-5160441 Msa1271710:CDS 40.0%
ATCAGCAGGTTTGAGAAGAA+GGG + chr8_3:5160423-5160442 Msa1271710:CDS 40.0%
! TGGATCCCAAACTTTGGAAA+TGG - chr8_3:5160684-5160703 None:intergenic 40.0%
TGGTGAATGATTCTTGAGAC+TGG + chr8_3:5160989-5161008 Msa1271710:intron 40.0%
TTGGTAGAGTACATTGTCTC+TGG + chr8_3:5161099-5161118 Msa1271710:CDS 40.0%
! TTGTTGCCATGATTTGGGAA+GGG + chr8_3:5161127-5161146 Msa1271710:CDS 40.0%
ATTCTTCCCTTCCCAAATCA+TGG - chr8_3:5161136-5161155 None:intergenic 40.0%
!! GTGACTTTGCTGTTGAGATT+GGG + chr8_3:5161226-5161245 Msa1271710:CDS 40.0%
GCACCTATTAGCTATTGGTA+AGG - chr8_3:5161565-5161584 None:intergenic 40.0%
ATTAGTTAAGGGACAGAGCT+TGG - chr8_3:5161725-5161744 None:intergenic 40.0%
TGATGCAGGAACGTTATTCA+TGG + chr8_3:5161817-5161836 Msa1271710:intron 40.0%
CCACAAACCAATTTCCTTGT+TGG - chr8_3:5161863-5161882 None:intergenic 40.0%
!! CCAACAAGGAAATTGGTTTG+TGG + chr8_3:5161860-5161879 Msa1271710:CDS 40.0%
TTTACTCGTAAATCCACGAG+TGG - chr8_3:5161921-5161940 None:intergenic 40.0%
CACTCATAAAGTTCCAGCAA+AGG - chr8_3:5161984-5162003 None:intergenic 40.0%
!! TTGCGTTTTAGCCATTGATG+TGG + chr8_3:5162133-5162152 Msa1271710:three_prime_UTR 40.0%
TTTGCATAGACAGATCCCTT+AGG + chr8_3:5162304-5162323 Msa1271710:three_prime_UTR 40.0%
GATAGTTCCAACCAACCTAA+GGG - chr8_3:5162322-5162341 None:intergenic 40.0%
TGATAGTTCCAACCAACCTA+AGG - chr8_3:5162323-5162342 None:intergenic 40.0%
! GTTGCTGTTGTTGCTGTGTT+GGG - chr8_3:5159925-5159944 None:intergenic 45.0%
AAGCCTGATGGAGTTCAAAG+AGG + chr8_3:5160001-5160020 Msa1271710:CDS 45.0%
AGTCCTCTTTGAACTCCATC+AGG - chr8_3:5160007-5160026 None:intergenic 45.0%
!! AGGTTGGTGAGATTATCAGC+AGG + chr8_3:5160409-5160428 Msa1271710:intron 45.0%
AACTCGTGGATCCCAAACTT+TGG - chr8_3:5160690-5160709 None:intergenic 45.0%
CACTTATTGGGTGAAACTCG+TGG - chr8_3:5160704-5160723 None:intergenic 45.0%
GATTCTTGAGACTGGACTTC+AGG + chr8_3:5160997-5161016 Msa1271710:intron 45.0%
TCTGCAAAGCCTTTCTTCAG+CGG + chr8_3:5161075-5161094 Msa1271710:CDS 45.0%
AAAGCCTTTCTTCAGCGGAT+TGG + chr8_3:5161080-5161099 Msa1271710:CDS 45.0%
TCTACCAATCCGCTGAAGAA+AGG - chr8_3:5161087-5161106 None:intergenic 45.0%
CCCAAATCATGGCAACAACA+GGG - chr8_3:5161125-5161144 None:intergenic 45.0%
CCCTGTTGTTGCCATGATTT+GGG + chr8_3:5161122-5161141 Msa1271710:CDS 45.0%
TCCCAAATCATGGCAACAAC+AGG - chr8_3:5161126-5161145 None:intergenic 45.0%
! GTTGTTGCCATGATTTGGGA+AGG + chr8_3:5161126-5161145 Msa1271710:CDS 45.0%
GGGAAGAATGTTGTGACAAC+TGG + chr8_3:5161147-5161166 Msa1271710:CDS 45.0%
AAGTCACCACGAATAGTTCC+AGG - chr8_3:5161213-5161232 None:intergenic 45.0%
!! GGTGACTTTGCTGTTGAGAT+TGG + chr8_3:5161225-5161244 Msa1271710:CDS 45.0%
!! ACTTTGCTGTTGAGATTGGG+AGG + chr8_3:5161229-5161248 Msa1271710:CDS 45.0%
!! TTCTGTTGAAAGCGCCAACA+AGG + chr8_3:5161846-5161865 Msa1271710:CDS 45.0%
!! GAAAGCGCCAACAAGGAAAT+TGG + chr8_3:5161853-5161872 Msa1271710:CDS 45.0%
!! AATTGGTTTGTGGTTCCCTG+AGG + chr8_3:5161870-5161889 Msa1271710:CDS 45.0%
!! ATTGGTTTGTGGTTCCCTGA+GGG + chr8_3:5161871-5161890 Msa1271710:CDS 45.0%
GCAAATTCCCGATCAACTTC+AGG - chr8_3:5161950-5161969 None:intergenic 45.0%
!!! GCCCTATTTTGATCCTTTGC+TGG + chr8_3:5161968-5161987 Msa1271710:three_prime_UTR 45.0%
CATAGACAGATCCCTTAGGT+TGG + chr8_3:5162308-5162327 Msa1271710:three_prime_UTR 45.0%
! TGTGGTGGTGTGAGAGGAAA+GGG - chr8_3:5159889-5159908 None:intergenic 50.0%
! TTGGGAGAGAGTGTGATGTG+TGG - chr8_3:5159907-5159926 None:intergenic 50.0%
! GGTTGCTGTTGTTGCTGTGT+TGG - chr8_3:5159926-5159945 None:intergenic 50.0%
GCCCTGTTGTTGCCATGATT+TGG + chr8_3:5161121-5161140 Msa1271710:CDS 50.0%
CTCAGATTGAGCAGGGTTTG+TGG - chr8_3:5161190-5161209 None:intergenic 50.0%
TTCCAGGCTCAGATTGAGCA+GGG - chr8_3:5161197-5161216 None:intergenic 50.0%
TCTGAGCCTGGAACTATTCG+TGG + chr8_3:5161204-5161223 Msa1271710:CDS 50.0%
ACTCGTAAATCCACGAGTGG+AGG - chr8_3:5161918-5161937 None:intergenic 50.0%
!! GACAGATCCCTTAGGTTGGT+TGG + chr8_3:5162312-5162331 Msa1271710:three_prime_UTR 50.0%
! TGGTGGTGTGAGAGGAAAGG+GGG - chr8_3:5159887-5159906 None:intergenic 55.0%
! GTGGTGGTGTGAGAGGAAAG+GGG - chr8_3:5159888-5159907 None:intergenic 55.0%
! GTGTGGTGGTGTGAGAGGAA+AGG - chr8_3:5159890-5159909 None:intergenic 55.0%
! GTGATGTGTGGTGGTGTGAG+AGG - chr8_3:5159895-5159914 None:intergenic 55.0%
! GGAGAGAGTGTGATGTGTGG+TGG - chr8_3:5159904-5159923 None:intergenic 55.0%
AACCCTGCTCAATCTGAGCC+TGG + chr8_3:5161192-5161211 Msa1271710:CDS 55.0%
GTTCCAGGCTCAGATTGAGC+AGG - chr8_3:5161198-5161217 None:intergenic 55.0%
GGTGGTGTGAGAGGAAAGGG+GGG - chr8_3:5159886-5159905 None:intergenic 60.0%
! AGGCTGCTTTGCCATGCAGC+AGG - chr8_3:5161898-5161917 None:intergenic 60.0%
TCCCTGAGGGACCTGCTGCA+TGG + chr8_3:5161884-5161903 Msa1271710:CDS 65.0%
GCCATGCAGCAGGTCCCTCA+GGG - chr8_3:5161888-5161907 None:intergenic 65.0%
TGCCATGCAGCAGGTCCCTC+AGG - chr8_3:5161889-5161908 None:intergenic 65.0%
GGCAAAGCAGCCTCCACTCG+TGG + chr8_3:5161905-5161924 Msa1271710:CDS 65.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 5159829 5162378 5159829 ID=Msa1271710;Name=Msa1271710
chr8_3 mRNA 5159829 5162378 5159829 ID=Msa1271710-mRNA-1;Parent=Msa1271710;Name=Msa1271710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=145|1|1|1|1|1|4|439|149
chr8_3 exon 5159829 5160027 5159829 ID=Msa1271710-mRNA-1:exon:1809;Parent=Msa1271710-mRNA-1
chr8_3 exon 5160411 5160459 5160411 ID=Msa1271710-mRNA-1:exon:1810;Parent=Msa1271710-mRNA-1
chr8_3 exon 5161019 5161250 5161019 ID=Msa1271710-mRNA-1:exon:1811;Parent=Msa1271710-mRNA-1
chr8_3 exon 5161825 5162378 5161825 ID=Msa1271710-mRNA-1:exon:1812;Parent=Msa1271710-mRNA-1
chr8_3 five_prime_UTR 5159829 5159973 5159829 ID=Msa1271710-mRNA-1:five_prime_utr;Parent=Msa1271710-mRNA-1
chr8_3 CDS 5159974 5160027 5159974 ID=Msa1271710-mRNA-1:cds;Parent=Msa1271710-mRNA-1
chr8_3 CDS 5160411 5160459 5160411 ID=Msa1271710-mRNA-1:cds;Parent=Msa1271710-mRNA-1
chr8_3 CDS 5161019 5161250 5161019 ID=Msa1271710-mRNA-1:cds;Parent=Msa1271710-mRNA-1
chr8_3 CDS 5161825 5161939 5161825 ID=Msa1271710-mRNA-1:cds;Parent=Msa1271710-mRNA-1
chr8_3 three_prime_UTR 5161940 5162378 5161940 ID=Msa1271710-mRNA-1:three_prime_utr;Parent=Msa1271710-mRNA-1
Gene Sequence

>Msa1271710

ATGGCTGAACAAACTTTCATCATGATCAAGCCTGATGGAGTTCAAAGAGGACTCGTTGGTGAGATTATCAGCAGGTTTGAGAAGAAGGGTTTCTACTTGAAAGGTTTGAAATTTTTGAATGTGGAACGTGCTTTTGCTGAGAAGCACTATGCTGATTTGTCTGCAAAGCCTTTCTTCAGCGGATTGGTAGAGTACATTGTCTCTGGCCCTGTTGTTGCCATGATTTGGGAAGGGAAGAATGTTGTGACAACTGGCAGAAAGATTATTGGTGCCACAAACCCTGCTCAATCTGAGCCTGGAACTATTCGTGGTGACTTTGCTGTTGAGATTGGGAGGAACGTTATTCATGGAAGTGATTCTGTTGAAAGCGCCAACAAGGAAATTGGTTTGTGGTTCCCTGAGGGACCTGCTGCATGGCAAAGCAGCCTCCACTCGTGGATTTACGAGTAA
Protein sequence

>Msa1271710

MAEQTFIMIKPDGVQRGLVGEIISRFEKKGFYLKGLKFLNVERAFAEKHYADLSAKPFFSGLVEYIVSGPVVAMIWEGKNVVTTGRKIIGATNPAQSEPGTIRGDFAVEIGRNVIHGSDSVESANKEIGLWFPEGPAAWQSSLHSWIYE*